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https://www.readbyqxmd.com/read/28791357/synergistic-effects-of-fibroblast-growth-factor-2-and-bone-morphogenetic-protein-2-on-bone-induction
#1
Rongying Song, Dingding Wang, Rong Zeng, Ju Wang
The present study investigated the synergistic effect of co‑administering fibroblast growth factor‑2 (FGF‑2) and bone morphogenetic protein‑2 (BMP‑2) on osteoblastic differentiation in C2C12 cells and in rats. C2C12 murine myoblast cells represent a well‑accepted in vitro model system to study the ability of BMP‑2 to alter cell lineage from the myogenic to the osteogenic phenotype. The osteoblastic differentiation potency was determined by alkaline phosphatase (ALP) and Alizarin red S staining...
August 7, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28765932/fgf18-inhibits-mc3t3%C3%A2-e1-cell-osteogenic-differentiation-via-the-erk-signaling-pathway
#2
Feng Zhai, Na Song, Jisheng Ma, Weiyue Gong, Haishan Tian, Xiaokun Li, Chao Jiang, Haijun Wang
Fibroblast growth factor (FGF) 18 is a member of the FGF family and serves a key role in skeletal growth and development. The present study investigated the effect of FGF18 on pre‑osteoblast MC3T3-E1 cells and the signaling pathways involved by performing an alkaline phosphatase (ALP) assay and reverse transcription‑quantitative polymerase chain reaction. MC3T3‑E1 cells incubated in a culture medium supplemented with FGF18 exhibited increased viability when compared with the untreated control cells. In addition, ALP activity was decreased in MC3T3‑E1 cells treated with FGF18 plus an osteogenic medium (OM) for 7 and 14 days when compared with untreated and OM‑treated controls...
July 26, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28729349/fgf9-prevents-pleural-fibrosis-induced-by-intra-pleural-adenovirus-injection-in-mice
#3
Aurélien Justet, Audrey Joannes, Valérie Besnard, Joëlle Marchal-Sommé, Madeleine Jaillet, Phillippe Bonniaud, Jean-Michel Sallenave, Brigitte Solhonne, Yves Castier, Pierre Mordant, Hervé Mal, Aurélie Cazes, Raphael Borie, Arnaud A Mailleux, Bruno Crestani
Fibroblast Growth Factor 9 (FGF9) is necessary for fetal lung development and is expressed by epithelium and mesothelium. We evaluated the role of FGF9 overexpression on adenoviral-induced pleural injury in vivo and determined the biological effects of FGF9 on mesothelial cells in vitro. We assessed the expression of FGF9 and FGF receptors by mesothelial cells in both Human and mouse lungs. Intrapleural injection of an adenovirus expressing human FGF9 (AdFGF9) or a control adenovirus (Adcont) was performed...
July 20, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28714570/human-osteoarthritic-chondrons-outnumber-patient-and-joint-matched-chondrocytes-in-hydrogel-culture-future-application-in-autologous-cell-based-oa-cartilage-repair
#4
Miriam Rothdiener, Tatiana Uynuk-Ool, Norbert Südkamp, Matthias Aurich, Alan J Grodzinsky, Bodo Kurz, Bernd Rolauffs
Autologous chondrocyte implantation (ACI) is used in 34-60% for osteoarthritic (OA) cartilage defects, although ACI is neither recommended nor designed for OA. Envisioning a hydrogel-based ACI for OA that uses chondrons instead of classically used chondrocytes, we hypothesized that human OA-chondrons may outperform OA-chondrocytes. We compared patient- and joint surface-matched human OA-chondrons vs. OA-chondrocytes cultured for the first time in a hydrogel, using a self-assembling peptide system. We determined yield, viability, cell numbers, mRNA expression, GAPDH mRNA enzyme activity, collagen II synthesis (CPII) and degradation (C2C), and sGAG...
July 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28701073/the-role-of-rhfgf-2-soaked-polymer-membrane-for-enhancement-of-guided-bone-regeneration
#5
Sang-Hoon Lee, Young-Bum Park, Hong-Seok Moon, June-Sung Shim, Han-Sung Jung, Hyung Jun Kim, Moon-Kyu Chung
The purposes of this study are to confirm the role of Fibroblast Growth Factor-2 (FGF-2) in bone regeneration by adding various concentrations of FGF-2 to the collagen membrane and applying it to the Biphasic Calcium Phosphate (BCP) bone graft site for guided bone regeneration, to explore the potential of collagen membrane as FGF-2 carrier, and to determine the optimum FGF concentration for enhancement of bone regeneration. Four bone defects of 8mm in diameter were created in 18 New Zealand rabbit calvaria...
July 12, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28696234/role-of-daptomycin-on-wound-healing-in-a-mrsa-burn-infected-animal-model
#6
Oriana Simonetti, Guendalina Lucarini, Fiorenza Orlando, Elisa Pierpaoli, Roberto Ghiselli, Mauro Provinciali, Pamela Castelli, Mario Guerrieri, Roberto Di Primio, Annamaria Offidani, Andrea Giacometti, Oscar Cirioni
Prolonged hospitalization and antibiotic therapy are risk factors for the development of methicillin-resistant S. aureus (MRSA) infections in thermal burn patients. We used a rat model to study the in vivo efficacy of daptomycin in the treatment of burn wound infections by S. aureus and we evaluated the wound healing process through morphological and immunohistochemical analysis. A copper bar heated in boiling water was applied on paraspinal site of each rat resulting in two full thickness burns. A small gauze was placed over each burn and inoculated with 5x10(7) cfu of S...
July 10, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28695797/effects-of-630-nm-red-and-460-nm-blue-light-emitting-diode-irradiation-on-healing-of-the-skin-wound-in-japanese-big-ear-white-rabbit
#7
Yanhong Li, Jigang Zhang, Yanfeng Xu, Yunlin Han, Binbin Jiang, Lan Huang, Hua Zhu, Yuhuan Xu, Weiling Yang, Chuan Qin
Objective To observe the effects of 630 nm red light and 460 nm blue light emitting diode irradiation on the healing of skin wounds in Japanese big-ear white rabbits. Methods The skin wound model was established with 8 Japanese big-ear white rabbits. Three parts of vulnus in each rabbit were used:two parts of vulnus were irradiated vertically by red and blue LED light,respectively(15 min/time),and the distance between lights and wounds was 15 cm;the 3(rd) part of the wound was used as a control. On the 21(st) day of the wounds exposure to light,the number of healing wounds and the percentage of healing area were recorded and the treatment effect of these two light sources was compared...
June 20, 2017: Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae
https://www.readbyqxmd.com/read/28682114/effectiveness-of-topical-application-of-ostrich-oil-on-the-healing-of-staphylococcus-aureus-and-pseudomonas-aeruginosa-infected-wounds
#8
Mohammad Reza Farahpour, Mahsa Vahid, Ahmad Oryan
BACKGROUND/AIMS: Management of infected wounds is one of the major challenges that surgeons and nurses face. Several antimicrobial agents have been used, but the toxicity, drug resistance, and their effect on the healing process remain a matter of concern. The present study was designed to analyze the accelerative impact of topical application of ostrich oil on infected wounds in a mouse model. MATERIALS AND METHODS: 72 BALB/c mice were divided into four main groups of control-sham, mupirocin, and two treatment groups receiving 2% and 4% (w/w) concentrations of ostrich oil, topically...
July 6, 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/28669805/in-vivo-diabetic-wound-healing-potential-of-nanobiocomposites-containing-bamboo-cellulose-nanocrystals-impregnated-with-silver-nanoparticles
#9
Rubbel Singla, Sourabh Soni, Vikram Patial, Pankaj Markand Kulurkar, Avnesh Kumari, Mahesh S, Yogendra S Padwad, Sudesh Kumar Yadav
In diabetes, hyperglycemic state immensely hinders the wound healing. Here, nanobiocomposites (NCs) developed by impregnation of in situ prepared silver nanoparticles in the matrix of bamboo cellulose nanocrystals were investigated for their ability to hasten the progress of healing events in streptozotocin induced diabetic mice model. Wounds treated with topically applied NCs (hydrogels) showed full recovery (98-100%) within 18days post wounding in contrast to the various control groups where incomplete healing (88-92%) was noticed...
June 29, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28556971/low-level-laser-therapy-780%C3%A2-nm-combined-with-collagen-sponge-scaffold-promotes-repair-of-rat-cranial-critical-size-defects-and-increases-tgf-%C3%AE-fgf-2-opg-rank-and-osteocalcin-expression
#10
Lana Sarita de Souza de Oliveira, Aurigena Antunes de Araújo, Raimundo Fernandes de Araújo Júnior, Carlos Augusto Galvão Barboza, Boniek Castillo Dutra Borges, José Sandro Pereira da Silva
The aim of this study was to evaluate the effect of collagen sponge scaffold (CSS) implantation associated with low-level laser therapy (LLLT) on repairing bone defects. A single 5-mm cranial defect was surgically created in forty Wistar rats, which then received one of the following four interventions (n = 10 per group): no treatment (G0); bone defect implanted with collagen sponge scaffold (CSS) alone (G1); defect treated with low-level laser therapy (LLLT) (wavelength 780 nm; total energy density 120 J/cm(2) ; power 50 mW) alone (G2); and CSS associated with LLLT treatment (G3)...
April 2017: International Journal of Experimental Pathology
https://www.readbyqxmd.com/read/28536636/a-simple-and-effective-protocol-for-fast-isolation-of-human-tenon-s-fibroblasts-from-a-single-trabeculectomy-biopsy-a-comparison-of-cell-behaviour-in-different-culture-media
#11
Agata Przekora, Tomasz Zarnowski, Grazyna Ginalska
BACKGROUND: Human Tenon's fibroblasts (HTFs) play a crucial role in wound healing. They cause postoperative scarring of the filtering bleb and are thus responsible for trabeculectomy failure. This study aimed to find an effective and fast protocol for HTF isolation from trabeculectomy biopsies. The protocol was compared with the commonly recommended HTF isolation procedure, which uses Dulbecco's modified Eagle's medium (DMEM). We used Eagle's minimum essential medium (EMEM) enriched with fibroblast growth factor (FGF), which selectively promoted the proliferation of HTF cells...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/28487375/pulmonary-fibrosis-requires-cell-autonomous-mesenchymal-fibroblast-growth-factor-fgf-signaling
#12
Robert D Guzy, Ling Li, Craig Smith, Samuel J Dorry, Hyun Young Koo, Lin Chen, David M Ornitz
Idiopathic pulmonary fibrosis (IPF) is characterized by progressive pulmonary scarring, decline in lung function, and often results in death within 3-5 five years after diagnosis. Fibroblast growth factor (FGF) signaling has been implicated in the pathogenesis of IPF; however, the mechanism through which FGF signaling contributes to pulmonary fibrosis remains unclear. We hypothesized that FGF receptor (FGFR) signaling in fibroblasts is required for the fibrotic response to bleomycin. To test this, mice with mesenchyme-specific tamoxifen-inducible inactivation of FGF receptors 1, 2, and 3 (Col1α2-CreER; TCKO mice) were lineage labeled and administered intratracheal bleomycin...
June 23, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28471382/decrease-of-mir-195-promotes-chondrocytes-proliferation-and-maintenance-of-chondrogenic-phenotype-via-targeting-fgf-18-pathway
#13
Yong Wang, Tao Yang, Yadong Liu, Wei Zhao, Zhen Zhang, Ming Lu, Weiguo Zhang
Slow growth and rapid loss of chondrogenic phenotypes are the major problems affecting chronic cartilage lesions. The role of microRNA-195 (miR-195) and its detailed working mechanism in the fore-mentioned process remains unknown. Fibroblastic growth factor 18 (FGF-18) plays a key role in cartilage homeostasis; whether miR-195 could regulate FGF-18 and its downstream signal pathway in chondrocyte proliferation and maintenance of chondrogenic phenotypes still remains unclear. The present research shows elevated miR-195 but depressed FGF-18 expressed in joint fluid specimens of 20 patients with chronic cartilage lesions and in CH1M and CH3M chondrocytes when compared with that in joint fluid specimens without cartilage lesions and in CH1W and CH2W chondrocytes, respectively...
May 4, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28470859/zirconium-oxide-and-niobium-oxide-used-as-radiopacifiers-in-a-calcium-silicate-based-material-stimulate-fibroblast-proliferation-and-collagen-formation
#14
G F Silva, J M Guerreiro-Tanomaru, T S da Fonseca, M I B Bernardi, E Sasso-Cerri, M Tanomaru-Filho, P S Cerri
AIM: To evaluate the influence of the addition of microparticulate (micro) and nanoparticulate (nano) zirconium oxide (ZrO2 ) and niobium pentoxide (Nb2 O5 ) to a calcium silicate-based cement (CS) on the subcutaneous healing process in rats compared with MTA Angelus™. METHODOLOGY: In each rat, two polyethylene tubes filled with the following materials: (i) MTA; (ii) CS + ZrO2 micro; (iii) CS + ZrO2 nano; (iv) CS + Nb2 O5 micro or (v) CS + Nb2 O5 nano were implanted subcutaneously; empty polyethylene tubes were used in the Control group...
May 4, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/28457186/heparin-augmentation-enhances-bioactive-properties-of-acellular-extracellular-matrix-scaffold
#15
Daniel Park, Holly Mewhort, Guoqi Teng, Darrell Belke, Jeannine Turnbull, Daniyil Svystonyuk, David Guzzardi, Sean Kang, Paul W M Fedak
Extracellular matrix (ECM) maintains a reservoir of bioactive growth factors and matricellular proteins that provide bioinductive effects on local cells that influence phenotype and behaviors. Bioactive acellular ECM scaffolds can be used therapeutically to stimulate adaptive tissue repair. Fibroblast growth factor-2 (FGF-2) attenuates transforming growth factor-β1 (TGF-β1) mediated cardiac fibrosis. Heparin glycosaminoglycan can influence FGF-2 bioactivity and could be leveraged to enhance tissue engineering strategies...
April 29, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28452589/lysyl-oxidase-propeptide-promotes-adipogenesis-through-inhibition-of-fgf-2-signaling
#16
John D Griner, Carl J Rogers, Mei-Jun Zhu, Min Du
Lysyl oxidase (LOX) catalyzes the oxidative deamination of lysine residues in collagen and elastin, key components of connective tissue. LOX is synthesized as an inactive 50 kD pre-proenzyme, and secreted to the extracellular matrix where it is cleaved into an active 32 kD LOX, and an 18kD free propeptide (LOX-PP), purportedly an inhibitor of fibroblast growth factor-2 (FGF-2) signaling. Given that adipocytes are distributed inside the connective tissue, it is likely that LOX-PP has an important regulatory role in adipogenesis, which has not been studied...
January 2, 2017: Adipocyte
https://www.readbyqxmd.com/read/28377320/do-il-3-gm-csf-effect-on-the-myofibroblastic-differentiation-of-human-adipose-derived-stromal-cells
#17
Jae-Sun Lee, Son-Seung Tae, Deok-Yeol Kim, Seung-Kyu Han, Woo-Kyung Kim, Eun-Sang Dhong
BACKGROUND: Capsular contracture is an incurable complication after silicone-based implant surgery. Myofibroblast is the predominant cell in the contracted capsule. We hypothesized that human adipose derive stromal cells (hASCs) together with fibroblast may show a similar phenotypic characteristics of myofibroblast after the treatment of inflammatory cytokines in vitro. MATERIALS AND METHODS: Interleukin 3 (IL-3) and granulocyte macrophage colony stimulating factor (GM-CSF) were treated in the culture of hASCs and HDFs...
April 2, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28339804/intra-cardiac-and-peripheral-levels-of-biochemical-markers-of-fibrosis-in-patients-undergoing-catheter-ablation-for-atrial-fibrillation
#18
Gordon A Begg, Rashed Karim, Tobias Oesterlein, Lee N Graham, Andrew J Hogarth, Stephen P Page, Christopher B Pepper, Kawal Rhode, Gregory Y H Lip, Arun V Holden, Sven Plein, Muzahir H Tayebjee
Aims: Measurement of circulating biomarkers of fibrosis may have a role in selecting patients and treatment strategy for catheter ablation. Pro-collagen type III N-terminal pro-peptide (PIIINP), C-telopeptide of type I collagen (ICTP), fibroblast growth factor 23 (FGF-23), and galectin 3 (gal-3) have all been suggested as possible biomarkers for this indication, but studies assessing whether peripheral levels reflect intra-cardiac levels are scarce. Methods and results: We studied 93 patients undergoing ablation for paroxysmal atrial fibrillation (AF) (n = 63) or non-paroxysmal AF (n = 30)...
February 27, 2017: Europace: European Pacing, Arrhythmias, and Cardiac Electrophysiology
https://www.readbyqxmd.com/read/28252047/inhibition-of-proliferation-and-epithelial-mesenchymal-transition-in-retinal-pigment-epithelial-cells-by-heavy-chain-hyaluronan-pentraxin-3
#19
Hua He, Ajay E Kuriyan, Chen-Wei Su, Megha Mahabole, Yuan Zhang, Ying-Ting Zhu, Harry W Flynn, Jean-Marie Parel, Scheffer C G Tseng
Proliferative vitreoretinopathy (PVR) is mediated by proliferation and epithelial mesenchymal transition (EMT) of retinal pigment epithelium (RPE). Because heavy chain-hyaluronic acid/pentraxin 3 (HC-HA/PTX3) purified from human amniotic membrane exerts anti-inflammatory and anti-scarring actions, we hypothesized that HC-HA/PTX3 could inhibit these PVR-related processes in vitro. In this study, we first optimized an ARPE-19 cell culture model to mimic PVR by defining cell density, growth factors, and cultivation time...
March 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28101973/heparin-cross-linked-collagen-sponge-scaffolds-improve-functional-regeneration-of-rat-tracheal-epithelium
#20
Ryosuke Nakamura, Akiko Tani, Susumu Yoshie, Masakazu Ikeda, Ikuo Wada, Akihiro Hazama, Yukio Nomoto, Yasuhiro Tada, Tatsuo Nakamura, Koichi Omori
Tracheal epithelial cells maintain airway homeostasis by mediating mucociliary clearance. Following tracheal reconstruction, timely epithelial regeneration is required to prevent respiratory compromise and infectious diseases. To achieve rapid tracheal epithelial regeneration, a heparin cross-linked collagen sponge containing fibroblast growth factor-2 (FGF-2) was prepared as a graft for tracheal reconstruction. The heparin cross-linked sponge exhibited a high FGF-2 retaining capacity, and tracheal epithelial and mesenchymal cells cultured in this sponge containing FGF-2 showed high proliferative capacities...
January 19, 2017: Journal of Tissue Engineering and Regenerative Medicine
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