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https://www.readbyqxmd.com/read/29552635/isolation-and-identification-of-proteins-secreted-by-cells-cultured-within-synthetic-hydrogel-based-matrices
#1
Lisa A Sawicki, Leila H Choe, Katherine L Wiley, Kelvin H Lee, April M Kloxin
Cells interact with and remodel their microenvironment, degrading large extracellular matrix (ECM) proteins (e.g., fibronectin, collagens) and secreting new ECM proteins and small soluble factors (e.g., growth factors, cytokines). Synthetic mimics of the ECM have been developed as controlled cell culture platforms for use in both fundamental and applied studies. However, how cells broadly remodel these initially well-defined matrices remains poorly understood and difficult to probe. In this work, we have established methods for widely examining both large and small proteins that are secreted by cells within synthetic matrices...
March 12, 2018: ACS Biomaterials Science & Engineering
https://www.readbyqxmd.com/read/29544382/released-fibroblast-growth-factor18-from-a-collagen-membrane-induces-osteoblastic-activity-involved-with-downregulation-of-mir-133a-and-mir-135a
#2
Kentaro Imamura, Keita Tachi, Tadahiro Takayama, Ryutaro Shohara, Hironori Kasai, Jisen Dai, Seiichi Yamano
We have developed a unique delivery system of growth factors using collagen membranes (CMs) to induce bone regeneration. We hypothesized that fibroblast growth factor18 (FGF-18), a pleiotropic protein that stimulates proliferation in several tissues, can be a good candidate to use our delivery system for bone regeneration. Cell viability, cell proliferation, alkaline phosphatase activity, mineralization, and marker gene expression of osteoblastic differentiation were evaluated after mouse preosteoblasts were cultured with a CM containing FGF-18, a CM containing platelet-derived growth factor, or a CM alone...
January 1, 2018: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/29540697/kaempferol-targeting-on-the-fibroblast-growth-factor-receptor-3-ribosomal-s6-kinase-2-signaling-axis-prevents-the-development-of-rheumatoid-arthritis
#3
Cheol-Jung Lee, Su-Jin Moon, Jeong-Hee Jeong, Sangbae Lee, Mee-Hyun Lee, Sun-Mi Yoo, Hye Suk Lee, Han Chang Kang, Joo Young Lee, Weon Sun Lee, Hee-Jin Lee, Eun-Kyung Kim, Joo-Yeon Jhun, Mi-La Cho, Jun-Ki Min, Yong-Yeon Cho
Rheumatoid arthritis (RA) is a systemic inflammatory disease that mainly affects the synovial joints. Although involvement of the fibroblast growth factor (FGF) signaling pathway has been suggested as an important modulator in RA development, no clear evidence has been provided. In this study, we found that synovial fluid basic FGF (bFGF) concentration was significantly higher in RA than in osteoarthritis (OA) patients. bFGF stimulates proliferation and migration of human fibroblast-like synoviocytes (FLSs) by activation of the bFGF-FGF receptor 3 (FGFR3)-ribosomal S6 kinase 2 (RSK2) signaling axis...
March 14, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29513041/vegf-mediated-angiogenesis-and-vascularization-of-a-fumarate-crosslinked-polycaprolactone-pclf-scaffold
#4
Eric R Wagner, Joshua Parry, Mahrokh Dadsetan, Dalibel Bravo, Scott M Riester, Andre J Van Wijnen, Michael J Yaszemski, Sanjeev Kakar
PURPOSE: Revascularization of natural and synthetic scaffolds is a critical part of the scaffold's incorporation and tissue ingrowth. Our goals were to create a biocompatible polymer scaffold with 3D-printing technology, capable of sustaining vascularization and tissue ingrowth. METHODS: We synthesized biodegradable polycaprolactone fumarate (PCLF) scaffolds to allow tissue ingrowth via large interconnected pores. The scaffolds were prepared with Poly(lactic-co-glycolic acid)(PLGA) microspheres seeded with or without different growth factors including VEGF,FGF-2, and/or BMP-2...
March 7, 2018: Connective Tissue Research
https://www.readbyqxmd.com/read/29467016/advancing-osteochondral-tissue-engineering-bone-morphogenetic-protein-transforming-growth-factor-and-fibroblast-growth-factor-signaling-drive-ordered-differentiation-of-periosteal-cells-resulting-in-stable-cartilage-and-bone-formation-in-vivo
#5
L F Mendes, H Katagiri, W L Tam, Y C Chai, L Geris, S J Roberts, F P Luyten
BACKGROUND: Chondrogenic mesenchymal stem cells (MSCs) have not yet been used to address the clinical demands of large osteochondral joint surface defects. In this study, self-assembling tissue intermediates (TIs) derived from human periosteum-derived stem/progenitor cells (hPDCs) were generated and validated for stable cartilage formation in vivo using two different animal models. METHODS: hPDCs were aggregated and cultured in the presence of a novel growth factor (GF) cocktail comprising of transforming growth factor (TGF)-β1, bone morphogenetic protein (BMP)2, growth differentiation factor (GDF)5, BMP6, and fibroblast growth factor (FGF)2...
February 21, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29449216/adjustable-delivery-of-pro-angiogenic-fgf-2-by-collagen-alginate-microspheres
#6
Zaheer Ali, Anik Islam, Peter Sherrell, Mark Le-Moine, Georgios Lolas, Konstantinos Syrigos, Mehrdad Rafat, Lasse D Jensen
Therapeutic induction of blood vessel growth (angiogenesis) in ischemic tissues holds great potential for treatment of myocardial infarction and stroke. Achieving sustained angiogenesis and vascular maturation has, however, been highly challenging. Here we demonstrate that collagen:alginate hydrogels containing therapeutic, pro-angiogenic FGF-2, and formulated as microspheres, is a promising and clinically relevant vehicle for therapeutic angiogenesis. By titrating the amount of readily dissolvable and degradable collagen with more slowly degradable alginate in the hydrogel mixture, the degradation rates of the biomaterial controlling the release kinetics of embedded pro-angiogenic FGF-2 can be adjusted...
February 15, 2018: Biology Open
https://www.readbyqxmd.com/read/29448134/enhancing-proliferation-and-optimizing-the-culture-condition-for-human-bone-marrow-stromal-cells-using-hypoxia-and-fibroblast-growth-factor-2
#7
Jung-Seok Lee, Seul Ki Kim, Byung-Joo Jung, Seong-Bok Choi, Eun-Young Choi, Chang-Sung Kim
This study aimed to determine the cellular characteristics and behaviors of human bone marrow stromal cells (hBMSCs) expanded in media in a hypoxic or normoxic condition and with or without fibroblast growth factor-2 (FGF-2) treatment. hBMSCs isolated from the vertebral body and expanded in these four groups were evaluated for cellular proliferation/migration, colony-forming units, cell-surface characterization, in vitro differentiation, in vivo transplantation, and gene expression. Culturing hBMSCs using a particular environmental factor (hypoxia) and with the addition of FGF-2 increased the cellular proliferation rate while enhancing the regenerative potential, modulated the multipotency-related processes (enhanced chondrogenesis-related processes/osteogenesis, but reduced adipogenesis), and increased cellular migration and collagen formation...
April 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29425341/fibroblast-growth-factor-23-is-induced-by-an-activated-renin-angiotensin-aldosterone-system-in-cardiac-myocytes-and-promotes-the-pro-fibrotic-crosstalk-between-cardiac-myocytes-and-fibroblasts
#8
Maren Leifheit-Nestler, Felix Kirchhoff, Julia Nespor, Beatrice Richter, Birga Soetje, Michael Klintschar, Joerg Heineke, Dieter Haffner
Background: Fibroblast growth factor 23 (FGF23) is discussed as a new biomarker of cardiac hypertrophy and mortality in patients with and without chronic kidney disease (CKD). We previously demonstrated that FGF23 is expressed by cardiac myocytes, enhanced in CKD and induces cardiac hypertrophy via activation of FGF receptor 4 independent of its co-receptor klotho. The impact of FGF23 on cardiac fibrosis is largely unknown. Methods: By conducting a retrospective case-control study including myocardial autopsy samples from 24 patients with end-stage CKD and in vitro studies in cardiac fibroblasts and myocytes, we investigated the pro-fibrotic properties of FGF23...
February 7, 2018: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/29405371/disclosing-the-physiology-of-dental-pulp-for-vital-pulp-therapy
#9
REVIEW
W L O da Rosa, E Piva, A F Silva
The discovery that dentine is a reservoir of bioactive molecules that can be recruited on demand has attracted efforts to develop new protocols and materials for vital pulp therapy (VPT). The non-collagenous proteins (NCPs) present in the dentine extracellular matrix (ECM) include growth factors (TGF-β1, BMP-7, FGF-2, IGF-1 and -2, NGF, and GDNF), extracellular matrix molecules (DSP, DPP, BSP, DMP-1, and DSPP), and both anti- and pro-inflammatory chemokines and cytokines (TNF-α, IL-1, IL-6, and IL-10). Molecules such as DSP and DPP are mainly expressed by odontoblasts, and they are cleaved products from dentine sialophosphoprotein (DSPP)...
February 6, 2018: International Endodontic Journal
https://www.readbyqxmd.com/read/29344510/ablation-of-fgfr2-in-fibroblasts-ameliorates-kidney-fibrosis-after-ischemia-reperfusion-injury-in-mice
#10
Zhuo Xu, Chunsun Dai
Background: Fibroblast growth factors (FGFs) are heparin-binding proteins involved in a variety of biological processes. However, the role and mechanisms of FGF/FGFR2 signaling in fibroblast activation and kidney fibrosis need further investigation. Methods: In this study, a mouse model with fibroblast-specific FGFR2 gene disruption was generated. The knockouts were born normal and no kidney dysfunction or histological abnormality was found within 2 months after birth...
December 2017: Kidney Diseases
https://www.readbyqxmd.com/read/29341894/protective-effects-of-antioxidin-rl-from-odorrana-livida-against-ultraviolet-b-irradiated-skin-photoaging
#11
Di Qin, Wen-Hui Lee, Zhiqin Gao, Weifen Zhang, Meiyu Peng, Tongyi Sun, Yuanyuan Gao
The unavoidable daily exposure of the skin to ultraviolet (UV) B radiation is proven to have deleterious effects. The action mechanism of antioxidin-RL, an antioxidant peptide purified from skin secretions of frog Odorrana livida with amino acid sequence of AMRLTYNRPCIYAT, is well characterized by NMR titration and mutation based on ABTS+ scavenging activities. In order to explore the protective effects of antioxidin-RL against UVB-irradiated skin photoaging, cell uptake assay was used to detect the location of antioxidin-RL molecules serving various biological functions in the cells...
January 13, 2018: Peptides
https://www.readbyqxmd.com/read/29313523/controlled-release-of-fgf-2-and-bmp-2-in-tissue-engineered-periosteum-promotes-bone-repair-in-rats
#12
Jie Yin, Sujun Qiu, Benchao Shi, Xiaolong Xu, Yunfei Zhao, Junhuai Gao, Shengli Zhao, Shaoxiong Min
The aim of this study was to prepare chitosan-collagen (CS/COL) scaffolds that could release fibroblast growth factor-2 (FGF-2) and bone morphogenetic protein 2 (BMP-2), and to study the effect of this scaffold on bone repair. By improving the double emulsion/solvent evaporation technique, BMP-2 was encapsulated in poly(lactic acid)-poly(ethylene glycol)-poly(lactic acid) (PELA) microcapsules, to the surface of which FGF-2 was attached. The CS/COL scaffold carrying the microcapsules was prepared by freeze-drying...
January 9, 2018: Biomedical Materials
https://www.readbyqxmd.com/read/29215029/mtorc1-signaling-is-a-critical-regulator-of-postnatal-tendon-development
#13
Joohyun Lim, Elda Munivez, Ming-Ming Jiang, I-Wen Song, Francis Gannon, Douglas R Keene, Ronen Schweitzer, Brendan H Lee, Kyu Sang Joeng
Tendons transmit contractile forces between musculoskeletal tissues. Whereas the biomechanical properties of tendons have been studied extensively, the molecular mechanisms regulating postnatal tendon development are not well understood. Here we examine the role of mTORC1 signaling in postnatal tendon development using mouse genetic approaches. Loss of mTORC1 signaling by removal of Raptor in tendons caused severe tendon defects postnatally, including decreased tendon thickness, indicating that mTORC1 is necessary for postnatal tendon development...
December 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29214350/co-culture-of-human-fibroblasts-and-borrelia-burgdorferi-enhances-collagen-and-growth-factor-mrna
#14
Elisabeth Aberer, Milana Surtov-Pudar, Daniel Wilfinger, Alexander Deutsch, Gerd Leitinger, Helmut Schaider
Skin fibrosis has been reported in Borrelia burgdorferi infection in Europe, but has been questioned by several authors. The objective of the present study was to examine the interaction of skin fibroblasts with B. burgdorferi sensu stricto B31 (BB) and B. afzelii (BA) in vitro by electron microscopy. We also determined the expression of collagen type I, TGF-β, FGF-1, calreticulin (CALR), decorin (DCN), and PDGF-α at the mRNA level in Borrelia/fibroblast co-cultures. Intact Borrelia attach to and transmigrate fibroblasts, and undergo cystic transformation outside the fibroblasts...
March 2018: Archives of Dermatological Research
https://www.readbyqxmd.com/read/28976339/effects-of-keratinocytes-differentiated-from-embryonic-and-adipogenic-stem-cells-on-wound-healing-in-a-diabetic-mouse-model
#15
Şükrü Kasap, Ali Barutçu, Hasan Güç, Şamil Yazgan, Müjde Kıvanç, H Seda Vatansever
OBJECTIVE: The current study aims to assess the molecular effects of keratinocytes derived from embryonic and adipose-derived stem cells (ADSCs) on wound healing in mice with diabetes mellitus. MATERIALS AND METHODS: Sixty BALB/c mice were randomly allocated into 6 groups of 10. Following diabetes mellitus induction by intraperitoneal injection of streptozocin, wounds were created and covered with gauze dipped in various solutions: isotonic saline, carrier and transfer medium-engineered dermal template, keratinocytes derived from embryonic stem cells (ESCs), keratinocytes differentiated from ADSCs, or ADSC medium alone...
November 2017: Wounds: a Compendium of Clinical Research and Practice
https://www.readbyqxmd.com/read/28946792/-calvarial-bone-regeneration-is-enhanced-by-sequential-delivery-of-fgf-2-and-bmp-2-from-layer-by-layer-coatings-with-a-biomimetic-calcium-phosphate-barrier-layer
#16
Gloria Gronowicz, Emily Jacobs, Tao Peng, Li Zhu, Marja Hurley, Liisa T Kuhn
A drug delivery coating for synthetic bone grafts has been developed to provide sequential delivery of multiple osteoinductive factors to better mimic aspects of the natural regenerative process. The coating is composed of a biomimetic calcium phosphate (bCaP) layer that is applied to a synthetic bone graft and then covered with a poly-l-Lysine/poly-l-Glutamic acid polyelectrolyte multilayer (PEM) film. Bone morphogenetic protein-2 (BMP-2) was applied before the coating process directly on the synthetic bone graft and then, bCaP-PEM was deposited followed by adsorption of fibroblast growth factor-2 (FGF-2) into the PEM layer...
December 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28940204/adipose-derived-mesenchymal-stromal-cells-prevented-rat-vocal-fold-scarring
#17
Tsuyoshi Morisaki, Yo Kishimoto, Ichiro Tateya, Yoshitaka Kawai, Ryo Suzuki, Takuya Tsuji, Nao Hiwatashi, Tatsuo Nakamura, Koichi Omori, Hiroya Kitano, Hiromi Takeuchi, Shigeru Hirano
OBJECTIVES/HYPOTHESIS: This study aimed to reveal the effects of adipose-derived mesenchymal stromal cells (ASCs) on prevention of vocal fold scarring by investigating how the immediate ASCs transplantation into the injured rat vocal fold affect the levels of gene transcription and translation. STUDY DESIGN: Prospective animal experiments with controls. METHODS: ASCs harvested from green fluorescent protein transgenic rat (ASCs group) or saline (sham group) were injected into the thyroarytenoid muscle of Sprague-Dawley rats immediately after stripping the vocal fold...
January 2018: Laryngoscope
https://www.readbyqxmd.com/read/28939209/calcium-and-bone-metabolism-indices
#18
Lu Song
Calcium and inorganic phosphate are of critical importance for many body functions, thus the regulations of their plasma concentrations are tightly controlled by the concerted actions of reabsorption/excretion in the kidney, absorption in the intestines, and exchange from bone, the major reservoir for calcium and phosphate in the body. Parathyroid hormone (PTH) and 1,25-dihydroxyvitamin D (1,25(OH)2D) control calcium homeostasis, whereas PTH, 1,25(OH)2D, and bone-derived fibroblast growth factor 23 (FGF 23) control phosphate homeostasis...
2017: Advances in Clinical Chemistry
https://www.readbyqxmd.com/read/28922541/effects-of-collagen-peptides-intake-on-skin-ageing-and-platelet-release-in-chronologically-aged-mice-revealed-by-cytokine-array-analysis
#19
Hongdong Song, Ling Zhang, Yongkang Luo, Siqi Zhang, Bo Li
Action mechanisms underlying various biological activities of collagen peptides (CPs) remained to be elucidated. Cytokines may play an important role in mediating these health benefits of CPs. This study aimed to systemically examine the cytokines in skin and blood regulated by CPs intake. Thirteen-month-old female Kunming mice were administered with CPs for 2 months (0 or 400 mg/kg bodyweight/day). The cytokines in skin and plasma were analysed using a 53-cytokine array and corresponding ELISA kits. In skin, CPs intake significantly down-regulated placenta growth factor (PIGF-2), insulin-like growth factor (IGF)-binding protein (IGFBP) -2 and IGFBP-3, and up-regulated platelet factor 4 (PF4), serpin E1 and transforming growth factor (TGF)-β1 ...
January 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28895052/the-effects-of-periostin-in-a-rat-model-of-isoproterenol-mediated-cardiotoxicity
#20
Mahmut Sözmen, Alparslan K Devrim, Yonca B Kabak, Tuba Devrim, Mert Sudagidan
Periostin is an extracellular matrix protein from fasciclin family, and it plays an important role in the cell adhesion, migration, and growth of the organism. Periostin prevents apoptosis while stimulating cardiomyocytes. The present study was designed to investigate cardioprotective effects of the recombinant murine periostin peptide administration in a rat model of isoproterenol (ISO)-induced myocardial injury. The experiment was performed on 84 adult male Sprague Dawley rats in 4 groups (n = 21): control group (1), periostin-treated group (2), ISO-treated group (3), and ISO + periostin-treated group (4)...
September 11, 2017: Cardiovascular Toxicology
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