Ivana Beatrice Mânica da Cruz, Nathália Cardoso de Afonso Bonotto, Bárbara Osmarin Turra, Cibele Ferreira Teixeira, Verônica Farina Azzolin, Ednea Aguiar Maia Ribeiro, Jacqueline Da Costa Escobar Piccoli, Fernanda Barbisan
Rotenone (Ro), causes superoxide imbalance by inhibiting complex I of the mitochondrial electron transport chain, being able to serve as a model for functional skin aging by inducing cytofunctional changes in dermal fibroblasts prior to proliferative senescence. To test this hypothesis, we conducted an initial protocol to select a concentration of Ro (0.5, 1, 1.5, 2, 2.5, and 3 μM) that would induce the highest levels of the aging marker beta-galactosidase (β-gal) in human dermal HFF-1 fibroblasts after 72 h of culture, as well as a moderate increase in apoptosis and partial G1 arrestment...
June 30, 2023: Toxicology in Vitro: An International Journal Published in Association with BIBRA