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Fgf mmp

Ariel B Rydeen, Joshua S Waxman
Although retinoic acid (RA) teratogenicity has been investigated for decades, the mechanisms underlying RA-induced outflow tract (OFT) malformations are not understood. Here, we show zebrafish embryos deficient for Cyp26a1 and Cyp26c1 enzymes, which promote RA degradation, have OFT defects resulting from two mechanisms: first, a failure of second heart field (SHF) progenitors to join the OFT, instead contributing to the pharyngeal arch arteries (PAAs), and second, a loss of first heart field (FHF) ventricular cardiomyocytes due to disrupted cell polarity and extrusion from the heart tube...
November 2016: PLoS Biology
Ali Said Durmus, Mehmet Tuzcu, Oguzhan Ozdemir, Cemal Orhan, Nurhan Sahin, Ibrahim Hanifi Ozercan, James Richard Komorowski, Shakir Ali, Kazim Sahin
Arginine silicate inositol (ASI) complex is a composition of arginine, silicon, and inositol that has been shown to have beneficial effects on vascular health. This study reports the effects of an ASI ointment on wound healing in rats. A full-thickness excision wound was created by using a disposable 5 mm diameter skin punch biopsy tool. In this placebo-controlled study, the treatment group's wound areas were covered by 4 or 10 % ASI ointments twice a day for 5, 10, or 15 days. The rats were sacrificed either 5, 10, or 15 days after the wounds were created, and biopsy samples were taken for biochemical and histopathological analysis...
October 14, 2016: Biological Trace Element Research
Myung Jin Ban, Jae Hong Park, Jae Wook Kim, Ki Nam Park, Jae Yong Lee, Hee Kyung Kim, Seung Won Lee
Objectives: This study assessed the regenerative efficacy of basic fibroblast growth factor (FGF) in a rabbit model of chronic vocal fold scarring and then confirmed its utility and safety in a prospective trial of patients with this condition. Methods: FGF was injected three times, at 1-week intervals, into a chronic vocal fold scar created in a rabbit model. After 1 month, mRNA level of procollagen I, hyaluronic acid synthetase 2 (HAS 2), and matrix metalloproteinase 2 (MMP 2) were analyzed by real-time polymerase chain reaction...
September 27, 2016: Clinical and Experimental Otorhinolaryngology
Changyue Xue, Jiamin Xie, Dan Zhao, Shiyu Lin, Tengfei Zhou, Sirong Shi, Xiaoru Shao, Yunfeng Lin, Bofeng Zhu, Xiaoxiao Cai
OBJECTIVES: The aim of the study was to investigate the role of the JAK/STAT3 signalling pathway in angiogenesis. MATERIALS AND METHODS: The model established in vitro, involved a 3D collagen gel being implanted with endothelial cells (ECs) from red fluorescent protein-labelled mice, and adipose-derived stromal cells (ASCs) from green fluorescent protein-labelled mice. Phenomena of angiogenesis, after treatment by the inhibitor and the activator of JAK/STAT3 pathway respectively, were observed using confocal laser scanning microscopy...
February 2017: Cell Proliferation
Yohei Kobayashi, Yoshitomo Saita, Hirofumi Nishio, Hiroshi Ikeda, Yuji Takazawa, Masashi Nagao, Tomoiku Takaku, Norio Komatsu, Kazuo Kaneko
BACKGROUND: Platelet-rich plasma (PRP) therapy has become an increasingly popular treatment for orthopaedics and sports-related injuries, and various clinically available PRP preparation methods exist. However, the differences in PRP quality among numerous preparation methods remain unclear. Specifically, the benefit of including leukocytes in the PRP product remains controversial, and few studies have been conducted to evaluate the effects of the interaction between platelets and leukocytes on the growth factor concentrations...
September 2016: Journal of Orthopaedic Science: Official Journal of the Japanese Orthopaedic Association
S L K Bowers, P R Norden, G E Davis
During capillary network formation, ECs establish interconnecting tubes with defined lumens that reside within vascular guidance tunnels (physical spaces generated during EC tubulogenesis). Pericytes are recruited to EC tubes within these tunnels and capillary basement membrane deposition occurs to facilitate tube maturation. Here, we discuss molecular mechanisms controlling EC tubulogenesis demonstrating the involvement of integrins, MT1-MMP, extracellular matrix, Cdc42, Rac1, Rac2, k-Ras, Rap1b, and key downstream effectors including Pak2, Pak4, IQGAP1, MRCKβ, and Rasip1...
2016: Advances in Pharmacology
Heather L Menden, Sheng Xia, Sherry M Mabry, Angels Navarro, Michael F Nyp, Venkatesh Sampath
In premature infants, sepsis is associated with alveolar simplification manifesting as bronchopulmonary dysplasia. The redox-dependent mechanisms underlying sepsis-induced inflammation and alveolar remodeling in the immature lung remain unclear. We developed a neonatal mouse model of sepsis-induced lung injury to investigate whether nicotinamide adenine dinucleotide phosphate oxidase 2 (NOX2) regulates Toll-like receptor (TLR)-mediated inflammation and alveolar remodeling. Six-day-old NOX2(+/+) and NOX2(-/-) mice were injected with intraperitoneal LPS to induce sepsis...
December 2016: American Journal of Respiratory Cell and Molecular Biology
Jing Li, Miaosha Luo, Yan Wang, Boxin Shang, Lei Dong
The inhibition of cyclooxygenase (COX)-2 has been reported to suppress growth and induce apoptosis in human pancreatic cancer cells. Nevertheless, the precise biological mechanism of how celecoxib, a selective COX-2 inhibitor, regulates the growth and invasion of pancreatic tumors is not completely understood. It has been shown that fibroblast growth factor-2 (FGF-2) and its receptor levels correlate with the inhibition of cancer cell proliferation, migration and invasion in pancreatic ductal adenocarcinoma (PDAC)...
September 2016: Oncology Reports
E Peterová, A Mrkvicová, L Podmolíková, M Řezáčová, J Kanta
Rat liver myofibroblasts (MFB) are the key cells involved in the deposition of extracellular matrix in fibrotic liver. They were isolated by repeated passaging of non-parenchymal cell fraction and cultured in 3-dimensional (3D) collagen gel mimicking tissue. The transfer of MFB from plastic dishes to collagen resulted in the change in their shape from large and spread to slender with long extensions. The expression of transforming growth factor-beta1 (TGF-beta1) and of MFB markers, alpha-smooth muscle actin (alpha-SMA) and cellular fibronectin (EDA-FN), on protein level was significantly decreased in collagen gel...
November 8, 2016: Physiological Research
Dawei Zhang, Yongjun Huang, Zongwen Huang, Rongkai Zhang, Honggang Wang, Dong Huang
BACKGROUND Osteoclast formation is closely related to the immune system. FTY720, a new immunosuppressive agent, has some functions in immune regulation. Its main active ingredients become FTY-720P in vivo by phosphorylation modification. The objective of this study was to determine the effects of FTY-720 with various concentrations on osteoclasts in vitro. MATERIAL AND METHODS RAW264.7 cells and bone marrow-derived mononuclear phagocytes (BMMs) were treated with RANKL to obtain osteoclasts in vitro. To investigate the role of FTY-720 in osteoclast formation, trap enzyme staining was performed and the number of osteoclasts was counted...
June 26, 2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Ke Li, Baiyong Shen, Xi Cheng, Ding Ma, Xiaoqian Jing, Xinyu Liu, Weiping Yang, Chenghong Peng, Weihua Qiu
Fibroblast growth factor receptor 3 (FGFR3) plays important roles in cell proliferation, differentiation, and angiogenesis. FGFR3 is abnormally upregulated in hepatocellular carcinoma (HCC), where it correlates positively with clinicopathologic index, HCC differentiation, and advanced nuclear grade. In this study, we describe an aberrantly spliced transcript of FGFR3, termed FGFR3Δ7-9, was identified as a high frequency even in HCC. FGFR3Δ7-9 lacks exons encoding the immunoglobulin-like III domain and promoted the proliferation, migration, and metastasis of HCC cells both in vitro and in vivo Coimmunoprecipation and surface plasmon resonance assays demonstrated that the binding affinity of the aberrant FGFR3Δ7-9 receptor to FGFs was significantly higher than wild-type FGFR3IIIc Furthermore, FGFR3Δ7-9 could be self-activated by homodimerization and autophosphorylation even in the absence of ligand...
July 15, 2016: Cancer Research
Junzhou Tang, Nan Su, Siru Zhou, Yangli Xie, Junlan Huang, Xuan Wen, Zuqiang Wang, Quan Wang, Wei Xu, Xiaolan Du, Hangang Chen, Lin Chen
OBJECTIVE: Fibroblast growth factor (FGF) signaling is involved in articular cartilage homeostasis. This study was undertaken to investigate the role and mechanisms of FGF receptor 3 (FGFR-3) in the pathogenesis of osteoarthritis (OA) caused by surgery and aging in mice. METHODS: FGFR-3 was conditionally deleted or activated in articular chondrocytes in adult mice subjected to surgical destabilization of the medial meniscus (DMM). A mouse model of human achondroplasia was also used to assess the role of FGFR-3 in age-associated spontaneous OA...
October 2016: Arthritis & Rheumatology
Chien-Kuo Han, Yun-Chen Tien, Dennis Jine-Yuan Hsieh, Tsung-Jung Ho, Chao-Hung Lai, Yu-Lan Yeh, Cecilia Hsuan Day, Chia-Yao Shen, Hsi-Hsien Hsu, Jing-Ying Lin, Chih-Yang Huang
Severe and potentially fatal hypotension and cardiac contractile dysfunction are common symptoms in patients with sepsis. LPS was previously found to dramatically upregulate expression of fibrosis-related factors FGF-2, uPA, MMP-2, and MMP-9 in primary cardiac fibroblasts. MMPs are capable of denaturing and degrading fibrillar collagens and other components of the extracellular matrix (ECM). Studies have shown that dysregulation of expression of MMPs is associated with development of myocardial extracellular matrix remodeling and cardiac fibrosis, which contribute to progression of heart failure...
April 20, 2016: Environmental Toxicology
Björn Hegner, Theres Schaub, Rusan Catar, Angelika Kusch, Philine Wagner, Kirill Essin, Claudia Lange, Gabriela Riemekasten, Duska Dragun
INTRODUCTION: Obliterative vasculopathy and fibrosis are hallmarks of systemic sclerosis (SSc), a severe systemic autoimmune disease. Bone marrow-derived mesenchymal stromal cells (MSCs) from SSc patients may harbor disease-specific abnormalities. We hypothesized disturbed vascular smooth muscle cell (VSMC) differentiation with increased propensity towards myofibroblast differentiation in response to SSc-microenvironment defining growth factors and determined responsible mechanisms. METHODS: We studied responses of multipotent MSCs from SSc-patients (SSc-MSCs) and healthy controls (H-MSCs) to long-term exposure to CTGF, b-FGF, PDGF-BB or TGF-β1...
2016: PloS One
T G Shrihari, V Vasudevan, V Manjunath, D Devaraju
Periodontal disease caused chiefly by bacteria is characterized by inflammation, bacteremia, and a strong immune response. It is based on evidence that a continuous long-term exposure to oral bacteremia and bacterial toxins induces inflammatory immune response after immune evasion releases growth factors such as FGF, EGF, TGF-Beta, free radicals such as ROS and NOS, cytokines such as TNFAlfa, IL-1 Beta, IL-6; and matrix metalloproteinase such as MMP-9. Immature myeloid cells such as macrophages, dendritic cells and granulocytes involved in chronic inflammation and tumor progression through immunosuppressive activity against innate and adaptive immunity by factors such as iNOS, Arginase1 and ROS, activate major transcriptional factors such as NF-KB and STAT3 that could contribute to genetic instability, uncontrolled cell proliferation, angiogenesis, resistance to apoptosis, epithelial to mesenchymal transition, immunosuppression, invasion and metastasis...
January 2016: Gulf Journal of Oncology
Tao Liang, Yong-Fu Ma, Jian Chu, Dao-Xi Wang, Yang Liu
OBJECTIVE: To study the effect of lentivirus-mediated integrin αVβ3-shRNA on tumor growth of mice with lung cancer xenograft. METHODS: Lung cancer tissue, paracancer tissue and normal tissue were collected and integrin αVβ3 expression was detected; BALB/c nude mice were selected, divided into integrin αVβ3 knockdown group (KD group) and negative control group (NC group), and inoculated with cells stably infected by integrin αVβ3-shRNA lentivirus and cells stably infected by negative control-shRNA lentivirus, respectively, the growth of tumor tissue was continuously observed, and the number of apoptosis cells as well as the expression of angiogenesis, apoptosis and invasion genes in tumor tissue were detected...
February 2016: Asian Pacific Journal of Tropical Medicine
Justinas Grasys, Bong-Sung Kim, Norbert Pallua
BACKGROUND: Although fat grafting has emerged as a major force in plastic, reconstructive, and aesthetic surgery, some questions regarding its reliability and regenerative potential remain unanswered. OBJECTIVES: The authors examined the influence of three anatomic areas on various lipoaspirate properties to identify the most appropriate harvest site for fat-grafting procedures. METHODS: Lipoaspirates from 25 healthy patients were harvested from the abdomen, inner thigh, and knee...
July 2016: Aesthetic Surgery Journal
Y H Roh, W Kim, K U Park, J H Oh
OBJECTIVES: This study was conducted to evaluate the cytokine-release kinetics of platelet-rich plasma (PRP) according to different activation protocols. METHODS: Two manual preparation procedures (single-spin (SS) at 900 g for five minutes; double-spin (DS) at 900 g for five minutes and then 1500 g for 15 minutes) were performed for each of 14 healthy subjects. Both preparations were tested for platelet activation by one of three activation protocols: no activation, activation with calcium (Ca) only, or calcium with a low dose (50 IU per 1 ml PRP) of thrombin...
February 2016: Bone & Joint Research
Hyunghee Lee, Jeonghyun Choi, Soon Shik Shin, Michung Yoon
ETHNOPHARMACOLOGICAL RELEVANCE: Korean red ginseng (ginseng, Panax ginseng C.A. Meyer) is a famous traditional drug used in Korea for the treatment and prevention of obesity, type 2 diabetes, cancer, and liver and cardiovascular diseases. Menopause is strongly associated with many of the aforementioned metabolic diseases and increased visceral obesity. The aims of this study were to investigate whether ginseng inhibits obesity and related disorders in ovariectomized (OVX) C57BL/6J mice, which is a mouse model of postmenopausal women, and to determine the mechanism of action involved in this process...
February 3, 2016: Journal of Ethnopharmacology
Maurício Moreira da Silva, Wagner Eduardo Matheus, Patrick Vianna Garcia, Rafael Mamprim Stopiglia, Athanase Billis, Ubirajara Ferreira, Wagner José Fávaro
INTRODUCTION AND OBJECTIVES: Reactive Stroma (RStr) is observed in many human cancers and is related to carcinogenesis. The objectives of the present study were to stablish a relationship of the RStr microenvironment with prostate cancer (Pca) through a morphological and molecular characterization, and to identify a possible relationship between RStr with worse prognosis factors and occurrence of malignant prostatic stem cells. MATERIALS AND METHODS: Forty prostatic samples were selected from men with Pca diagnosis submitted to radical prostatectomy; they were divided in two groups: Group-1 (n=20): samples without reactive stroma; Group-2 (n=20): samples of PCa with intense stroma reaction...
September 2015: International Braz J Urol: Official Journal of the Brazilian Society of Urology
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