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https://www.readbyqxmd.com/read/28387977/ex-vivo-analysis-of-the-contribution-of-fgf10-cells-to-airway-smooth-muscle-cell-formation-during-early-lung-development
#1
Elie El Agha, Vahid Kheirollahi, Alena Moiseenko, Werner Seeger, Saverio Bellusci
BACKGROUND: Airway smooth muscle cells (ASMCs) have been widely studied during embryonic lung development. These cells have been shown to control epithelial bifurcation during branching morphogenesis. Fibroblast growth factor 10-positive (FGF10(+) ) cells, originally residing in the submesothelial mesenchyme, contribute to ASMC formation in the distal lung. The reported work aims at monitoring the response of FGF10(+) progenitors and differentiated ASMCs to growth factor treatment in real time using lineage tracing in the background of an air-liquid interface culture system...
April 7, 2017: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/28235676/the-mek1-inhibitors-uo126-and-pd98059-block-pdgf-ab-induced-phosphorylation-of-threonine-292-in-porcine-smooth-muscle-cells
#2
Thomas Kubin, Ayse Cetinkaya, Markus Schönburg, Andres Beiras-Fernandez, Thomas Walther, Manfred Richter
PDGF-AB and FGF-2 (GFs) induce smooth muscle cell (SMC) proliferation which is indispensible for arteriogenesis. While there is common agreement that GFs stimulate SMC proliferation through phosphorylation (P-) of MEK1/2 at Ser218/222, we previously demonstrated that the MEK inhibitors PD98059 and UO126 did not inhibit P-Ser218/222 as originally proposed but caused strong hyperphosphorylation. Here, we demonstrate that GFs increased phosphorylation of MEK1 at Thr292 while UO126 and PD98059 blocked this phosphorylation...
February 21, 2017: Cytokine
https://www.readbyqxmd.com/read/28209294/sulfated-glycosaminoglycans-and-non-classically-secreted-proteins-basic-fgf-and-epimorphin-coordinately-regulate-tgf-%C3%AE-induced-cell-behaviors-of-human-scar-dermal-fibroblasts
#3
Tomoatsu Horigome, Shinya Takumi, Kota Shirai, Takumi Kido, Natsumi Hagiwara-Chatani, Ayumi Nakashima, Naoki Adachi, Hiroko Yano, Yohei Hirai
BACKGROUND: Upon skin injuries, dermal fibroblasts actively produce transforming growth factor-β (TGF-β), which leads to the formation of α-smooth muscle actin (αSMA)-positive granulation tissues. The hyperplasia or incomplete regression of these tissues subsequently causes scar formation in the skin, where sulfated glycosaminoglycans (GAGs), side chains of unique proteoglycans, are supposed to play important roles. OBJECTIVE: The aim of this study is to clarify the effects of sulfated GAGs on dermal cell behaviors triggered by the TGF-β signaling, along with its possible regulators basic fibroblast growth factor (bFGF) and cell surface epimorphin...
February 3, 2017: Journal of Dermatological Science
https://www.readbyqxmd.com/read/27976512/fgf2-antagonizes-aberrant-tgf%C3%AE-regulation-of-tropomyosin-role-for-posterior-capsule-opacity
#4
Eri Kubo, Shinsuke Shibata, Teppei Shibata, Etsuko Kiyokawa, Hiroshi Sasaki, Dhirendra P Singh
Transforming growth factor (TGF) β2 and fibroblast growth factor (FGF) 2 are involved in regulation of posterior capsule opacification (PCO) and other processes of epithelial-mesenchymal transition (EMT) such as cancer progression, wound healing and tissue fibrosis as well as normal embryonic development. We previously used an in vivo rodent PCO model to show the expression of tropomyosin (Tpm) 1/2 was aberrantly up-regulated in remodelling the actin cytoskeleton during EMT. In this in vitro study, we show the Tpms family of cytoskeleton proteins are involved in regulating and stabilizing actin microfilaments (F-actin) and are induced by TGFβ2 during EMT in lens epithelial cells (LECs)...
May 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/27825029/the-role-of-fibroblast-growth-factor-2-in-patients-with-uterine-smooth-muscle-tumors-an-immunohistochemical-study
#5
Barbara Bodner-Adler, Klaus Mayerhofer, Klaus Czerwenka, Oliver Kimberger, Heinz Koelbl, Klaus Bodner
OBJECTIVE: Fibroblast growth factor 2 (FGF-2) is considered to be a potent stimulator of angiogenesis and seems therefore to play an important role in the growth of tumors. We compared the immunohistochemical profile of FGF-2 in patients with uterine leiomyomas, smooth muscle tumors of uncertain malignant potential (STUMP) and leiomyosarcoma (LMS). Furthermore, we tried to clarify the prognostic role of FGF-2 in uterine leiomyosarcoma. STUDY DESIGN: FGF-2 expression was investigated by immunohistochemistry from paraffin-embedded tissue in 26 patients with leiomyoma, in 24 cases with STUMP and in 21 patients with LMS...
December 2016: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/27634335/fibroblast-growth-factor-fgf-signaling-regulates-transforming-growth-factor-beta-tgf%C3%AE-dependent-smooth-muscle-cell-phenotype-modulation
#6
Pei-Yu Chen, Lingfeng Qin, Guangxin Li, George Tellides, Michael Simons
Smooth muscle cells (SMCs) in normal blood vessels exist in a highly differentiate state characterized by expression of SMC-specific contractile proteins ("contractile phenotype"). Following blood vessel injury in vivo or when cultured in vitro in the presence of multiple growth factors, SMC undergo a phenotype switch characterized by the loss of contractile markers and appearance of expression of non-muscle proteins ("proliferative phenotype"). While a number of factors have been reported to modulate this process, its regulation remains uncertain...
September 16, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27599364/fibroblast-growth-factor-23-inhibits-osteoblastic-gene-expression-and-induces-osteoprotegerin-in-vascular-smooth-muscle-cells
#7
Takehiro Nakahara, Keiko Kawai-Kowase, Hiroki Matsui, Hiroaki Sunaga, Toshihiro Utsugi, Tatsuya Iso, Masashi Arai, Shouichi Tomono, Masahiko Kurabayashi
BACKGROUND AND AIMS: Elevated fibroblast growth factor 23 (FGF23) levels are associated with cardiovascular mortality in patients with chronic kidney disease. However, both clinical and basic research have demonstrated conflicting evidence regarding the pathophysiological role of FGF23 in vascular calcification. The aim of this study was to determine the role of FGF23 in the osteoblastic gene expression in vascular smooth muscle cells (SMCs). METHODS AND RESULTS: We transduce human aortic SMCs (HASMCs) expressing klotho and FGF receptors with the adenovirus expressing human FGF23 (Ad-FGF23)...
October 2016: Atherosclerosis
https://www.readbyqxmd.com/read/27543985/differential-regulatory-role-of-soluble-klothos-on-cardiac-fibrogenesis-in-hypertension
#8
Xue Liu, Yuanjian Chen, Cody W McCoy, Tieqiang Zhao, Darryl L Quarles, Min Pi, Syamal K Bhattacharya, Gwendalyn King, Yao Sun
BACKGROUND: Soluble Klotho functions as an endocrine factor that plays important roles in a variety of pathophysiological processes. Soluble Klotho contains 130 KDa and 65 KDa isoforms. However, their distinct individual functional heterogeneity remains uncertain. Herein, we investigated the regulatory role of two soluble Klothos on cardiac fibrogenic responses. METHODS AND RESULTS: The effect of soluble Klothos on myofibroblast differentiation, proliferation, and collagen synthesis/degradation were examined in cultured mouse cardiac myofibroblasts...
October 2016: American Journal of Hypertension
https://www.readbyqxmd.com/read/27470512/fibroblast-growth-factor-12-is-a-novel-regulator-of-vascular-smooth-muscle-cell-plasticity-and-fate
#9
Sun-Hwa Song, Kyungjong Kim, Eun-Kyung Jo, Young-Wook Kim, Jin-Sook Kwon, Sun Sik Bae, Jong-Hyuk Sung, Sang Gyu Park, Jee Taek Kim, Wonhee Suh
OBJECTIVE: Vascular smooth muscle cells (VSMCs) modulate their phenotype between synthetic and contractile states in response to environmental changes; this modulation plays a crucial role in the pathogenesis of restenosis and atherosclerosis. Here, we identified fibroblast growth factor 12 (FGF12) as a novel key regulator of the VSMC phenotype switch. APPROACH AND RESULTS: Using murine models and human specimens, we found that FGF12 was highly expressed in contractile VSMCs of normal vessel walls but was downregulated in synthetic VSMCs from injured and atherosclerotic vessels...
September 2016: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/27429124/the-role-of-cytokines-tgf-beta1-and-fgf-1-in-the-expression-of-characteristic-markers-of-rat-liver-myofibroblasts-cultured-in-three-dimensional-collagen-gel
#10
COMPARATIVE STUDY
E Peterová, A Mrkvicová, L Podmolíková, M Řezáčová, J Kanta
Rat liver myofibroblasts (MFB) are the key cells involved in the deposition of extracellular matrix in fibrotic liver. They were isolated by repeated passaging of non-parenchymal cell fraction and cultured in 3-dimensional (3D) collagen gel mimicking tissue. The transfer of MFB from plastic dishes to collagen resulted in the change in their shape from large and spread to slender with long extensions. The expression of transforming growth factor-beta1 (TGF-beta1) and of MFB markers, alpha-smooth muscle actin (alpha-SMA) and cellular fibronectin (EDA-FN), on protein level was significantly decreased in collagen gel...
November 8, 2016: Physiological Research
https://www.readbyqxmd.com/read/27189169/smooth-muscle-fgf-tgf%C3%AE-cross-talk-regulates-atherosclerosis-progression
#11
Pei-Yu Chen, Lingfeng Qin, Guangxin Li, George Tellides, Michael Simons
The conversion of vascular smooth muscle cells (SMCs) from contractile to proliferative phenotype is thought to play an important role in atherosclerosis. However, the contribution of this process to plaque growth has never been fully defined. In this study, we show that activation of SMC TGFβ signaling, achieved by suppression of SMC fibroblast growth factor (FGF) signaling input, induces their conversion to a contractile phenotype and dramatically reduces atherosclerotic plaque size. The FGF/TGFβ signaling cross talk was observed in vitro and in vivo In vitro, inhibition of FGF signaling increased TGFβ activity, thereby promoting smooth muscle differentiation and decreasing proliferation...
July 2016: EMBO Molecular Medicine
https://www.readbyqxmd.com/read/27123814/tracheal-smooth-muscle-cells-stimulated-by-stem-cell-factor-c-kit-coordinate-the-production-of-transforming-growth-factor-%C3%AE-1-and-fibroblast-growth-factor-2-mediated-by-chemokine-c-c-motif-ligand-3
#12
Luis Cezar Farias de Oliveira, Taís Marolato Danilucci, Antonio Hernandes Chaves-Neto, Ana Paula Campanelli, Tereza Cristina Cardoso da Silva, Sandra Helena Penha Oliveira
The aim of this study was to evaluate the mechanism involved in the stem cell factor (SCF)-induced production of fibroblast growth factor-2 (FGF-2), transforming growth factor-β1 (TGF-β1), and chemokine (C-C motif) ligand 3 (CCL3) in tracheal smooth muscle cells (tSMCs) and the signaling pathway involved in the process. tSMC primary cultures were stimulated with SCF and evaluated at 24 h. Cells treated with specific antibodies did not show any immunolabeling for cytokeratin or fibroblast activation protein, but were positive for α-smooth muscle actin, indicating the purity of the primary cell line...
June 2016: Journal of Interferon & Cytokine Research
https://www.readbyqxmd.com/read/27054717/intrinsic-deregulation-of-vascular-smooth-muscle-and-myofibroblast-differentiation-in-mesenchymal-stromal-cells-from-patients-with-systemic-sclerosis
#13
Björn Hegner, Theres Schaub, Rusan Catar, Angelika Kusch, Philine Wagner, Kirill Essin, Claudia Lange, Gabriela Riemekasten, Duska Dragun
INTRODUCTION: Obliterative vasculopathy and fibrosis are hallmarks of systemic sclerosis (SSc), a severe systemic autoimmune disease. Bone marrow-derived mesenchymal stromal cells (MSCs) from SSc patients may harbor disease-specific abnormalities. We hypothesized disturbed vascular smooth muscle cell (VSMC) differentiation with increased propensity towards myofibroblast differentiation in response to SSc-microenvironment defining growth factors and determined responsible mechanisms. METHODS: We studied responses of multipotent MSCs from SSc-patients (SSc-MSCs) and healthy controls (H-MSCs) to long-term exposure to CTGF, b-FGF, PDGF-BB or TGF-β1...
2016: PloS One
https://www.readbyqxmd.com/read/26875593/vein-graft-thrombi-a-niche-for-smooth-muscle-cell-colonization-a-hypothesis-to-explain-the-asymmetry-of-intimal-hyperplasia
#14
I Blaas, K Heinz, P Würtinger, A Türkcan, C Tepeköylü, M Grimm, C Doppler, K Danzl, B Messner, D Bernhard
UNLABELLED: Essentials Vein graft failure is the most frequent late onset complication of coronary artery bypass grafting. Cuff technique-based interposition mouse model including new anticoagulation regime was conducted. Early vein graft thrombi may serve as a niche for smooth muscle cell colonization. The focal character of early thrombi may form the basis for the asymmetry of intimal hyperplasia. SUMMARY: Background Autologous saphenous veins are widely used in coronary artery bypass grafting; however, 10 years after surgery, 40% of grafts are completely occluded, and another 30% show reduced blood flow...
May 2016: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/26860892/ponatinib-attenuates-experimental-pulmonary-arterial-hypertension-by-modulating-wnt-signaling-and-vasohibin-2-vasohibin-1
#15
Zechun Kang, Yunxia Ji, Guanghua Zhang, Yubei Qu, Liang Zhang, Wanglin Jiang
AIMS: An abnormal ratio of vasohibin-2/vasohibin-1 may be involved in the abnormal angiogenesis and vascular remodeling during pulmonary arterial hypertension (PAH). MAIN METHODS: To evaluate the pharmacological actions of Ponatinib (AP) in experimental model of PAH, the effects of AP on TGF-β1-mediated endothelial-mesenchymal transition (EndoMT) in human pulmonary microvascular endothelial cells (HPMEC), and the hypoxic human pulmonary artery smooth muscle cells (HPASMC) proliferation and HPMEC in vitro, and on bleomycin (BLM)-induced PAH in vivo were investigated...
March 1, 2016: Life Sciences
https://www.readbyqxmd.com/read/26828864/comparative-systems-genetics-view-of-endometriosis-and-uterine-leiomyoma-two-sides-of-the-same-coin
#16
COMPARATIVE STUDY
Vladislav S Baranov, Tatyana E Ivaschenko, Maria I Yarmolinskaya
Endometriosis (EM) and uterine leiomyoma (UL) are two most frequent benign tumors of monoclonal origin affecting about 30% of all women in their reproductive age. Modern molecular technologies have made a tremendous impact in understanding both disorders. Here is the first comparative analysis of molecular mechanisms underlying development of EM and UL as it looks from the platform of systems genetics. Similarities and differences of EM and UL at their incipient stages are enlightened with special emphasis on their gene networks, gene expression, and epigenetic regulation, of pathologic development...
2016: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/26828526/role-of-emt-in-metastasis-and-therapy-resistance
#17
REVIEW
Bethany N Smith, Neil A Bhowmick
Epithelial-mesenchymal transition (EMT) is a complex molecular program that regulates changes in cell morphology and function during embryogenesis and tissue development. EMT also contributes to tumor progression and metastasis. Cells undergoing EMT expand out of and degrade the surrounding microenvironment to subsequently migrate from the primary site. The mesenchymal phenotype observed in fibroblasts is specifically important based on the expression of smooth muscle actin (α-SMA), fibroblast growth factor (FGF), fibroblast-specific protein-1 (FSP1), and collagen to enhance EMT...
2016: Journal of Clinical Medicine
https://www.readbyqxmd.com/read/26682010/fibroblast-growth-factor-9-activates-c-kit-progenitor-cells-and-enhances-angiogenesis-in-the-infarcted-diabetic-heart
#18
Dinender Singla, Jing Wang
We hypothesized that fibroblast growth factor-9 (FGF-9) would enhance angiogenesis via activating c-kit positive stem cells in the infarcted nondiabetic and diabetic heart. In brief, animals were divided into three groups: Sham, MI, and MI+FGF-9. Two weeks following MI or sham surgery, our data suggest that treatment with FGF-9 significantly diminished vascular apoptosis compared to the MI group in both C57BL/6 and db/db mice (p < 0.05). Additionally, the number of c-kit(+ve)/SM α-actin(+ve) cells and c-kit(+ve)/CD31(+ve) cells were greatly enhanced in the MI+FGF-9 groups relative to the MI suggesting FGF-9 enhances c-Kit cell activation and their differentiation into vascular smooth muscle cells and endothelial cells, respectively (p < 0...
2016: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/26592322/fibroblast-growth-factor-21-attenuates-hepatic-fibrogenesis-through-tgf-%C3%AE-smad2-3-and-nf-%C3%AE%C2%BAb-signaling-pathways
#19
Pengfei Xu, Yingjie Zhang, Yunye Liu, Qingyan Yuan, Liying Song, Mingyao Liu, Zhihang Liu, Yongbi Yang, Junyan Li, Deshan Li, Guiping Ren
Fibroblast growth factor 21 (FGF-21) is a secreted protein, which has anti-diabetic and lipocaic effects, but its ability to protect against hepatic fibrosis has not been studied. In this study, we investigated the ability of FGF-21 to attenuate dimethylnitrosamine (DMN)-induced hepatic fibrogenesis in mice and the mechanism of its action. Hepatic fibrosis was induced by injection of DMN, FGF-21 was administered to the mice once daily in association with DMN injection till the end of the experiment. Histopathological examination, tissue 4-hydroxyproline content and expressions of smooth muscle α-actin (α-SMA) and collagen I were measured to assess hepatic fibrosis...
January 1, 2016: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/26535780/inhibition-of-fgfr-signaling-with-pd173074-ameliorates-monocrotaline-induced-pulmonary-arterial-hypertension-and-rescues-bmpr-ii-expression
#20
Yaguo Zheng, Hong Ma, Enci Hu, Zhiwei Huang, Xiaoling Cheng, Changming Xiong
BACKGROUND: Numerous studies have demonstrated that fibroblast growth factor-2 (FGF-2) signaling may play a pivotal role in the development of pulmonary arterial hypertension (PAH). Excessive endothelial FGF-2 contributes to smooth muscle hyperplasia and disease progression. PD173074 is a potent FGF receptor 1 (FGFR-1) inhibitor that displays high activity and selectivity. The aim of this study was to investigate the effects of PD173074 on monocrotaline-induced PAH. We also evaluated whether FGFR-1 inhibition could attenuate bone morphogenetic protein type II receptor (BMPR-II) downregulation in the monocrotaline model...
November 2015: Journal of Cardiovascular Pharmacology
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