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https://www.readbyqxmd.com/read/29139141/developing-novel-species-specific-dna-markers-for-pcr-based-species-identification-of-the-lactobacillus-sakei-group
#1
Chien-Hsun Huang, Jong-Shian Liou, Lina Huang, Koichi Watanabe
Identification members of the Lactobacillus sakei group (LSG) by common phenotypic and genotypic methods is generally inadequate and time-consuming. The objective of this study was to develop novel species-specific primers based on sequence-characterized amplified region (SCAR) markers using random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) analysis. Three species-specific fragments were gel-purified, cloned, and sequenced after preliminary screening of 80 random primers. Accordingly, three pairs of primers Lcur-F/R, Lgram-F/R, and Lsakei-F/R were designed based on single species-specific bands (281, 278, and 472 bp) that were obtained from L...
November 14, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29122706/mid-to-long-term-outcomes-of-laparoscopic-sleeve-gastrectomy-in-indian-population-3-7-year-results-a-retrospective-cohort-study
#2
Harshit Garg, Sandeep Aggarwal, Mahesh Chandra Misra, Pratyusha Priydarshini, Ashish Swami, Lokesh Kashyap, Richa Jaiswal
INTRODUCTION: Few studies have addressed the mid to long term impact of Laparoscopic Sleeve Gastrectomy (LSG) on weight loss and obesity associated co-morbidities, particularly in Indian population. The aim of this study is to assess the efficacy of LSG in morbid obesity over 3-7 years follow up. MATERIALS AND METHODS: Data of all patients who underwent LSG between January 2008 and March 2015 and completed their at least 1 year follow up till March 2016 was retrospectively reviewed using a prospectively collected database...
November 6, 2017: International Journal of Surgery
https://www.readbyqxmd.com/read/29119692/impact-of-same-day-vs-day-before-pre-operative-lymphoscintigraphy-for-sentinel-lymph-node-biopsy-for-early-breast-cancer-local-australian-experience
#3
Yang Yang Huang, Amelie Maurel, Saud Hamza, Lee Jackson, Zeyad Al-Ogaili
INTRODUCTION: To assess the impact of delayed vs immediate pre-operative lymphoscintigraphy (LSG) for sentinel lymph node biopsy in a single Australian tertiary breast cancer centre. METHODS: Retrospective cohort study analysing patients with breast cancer or DCIS who underwent lumpectomy or mastectomy with pre-operative LSG and intra-operative sentinel lymph node biopsy from January 2015 to June 2016. RESULTS: A total of 182 LSG were performed...
November 9, 2017: Journal of Medical Imaging and Radiation Oncology
https://www.readbyqxmd.com/read/29119336/bariatric-surgery-as-an-efficient-treatment-for-non-alcoholic-fatty-liver-disease-in-a-prospective-study-with-1-year-follow-up-bariscan-study
#4
Felix Nickel, Christian Tapking, Laura Benner, Janina Sollors, Adrian T Billeter, Hannes G Kenngott, Loay Bokhary, Mathias Schmid, Moritz von Frankenberg, Lars Fischer, Sebastian Mueller, Beat P Müller-Stich
BACKGROUND: Bariatric surgery gains attention as a potential treatment for non-alcoholic fatty liver disease (NAFLD). The present study aimed to evaluate improvement of NAFLD after the two most common bariatric procedures with validated non-invasive instruments. MATERIAL AND METHODS: N = 100 patients scheduled for laparoscopic sleeve gastrectomy (LSG) or Roux-en-Y gastric bypass (RYGB) were included. NAFLD was evaluated preoperatively and postoperatively with liver stiffness measurement by transient elastography and laboratory-based fibrosis scores...
November 9, 2017: Obesity Surgery
https://www.readbyqxmd.com/read/29116560/preoperative-and-postoperative-assessments-of-biochemical-parameters-in-patients-with-severe-obesity-undergoing-laparoscopic-sleeve-gastrectomy
#5
Deborah M Gregory, Laurie K Twells, Kendra K Lester, William K Midodzi, Mette Rode Pedersen, David Pace, Chris Smith, Darrell Boone, Edward W Randell, Christopher S Kovacs
BACKGROUND: The study aim was to determine the prevalence of abnormal serum biochemistries associated with micronutrient deficiencies before and after laparoscopic sleeve gastrectomy (LSG). METHODS: Two hundred and one patients had LSG surgery between May 2011 and May 2014. Using a prospective cohort study design, data were collected on ferritin, hemoglobin (Hgb), mean cell volume (MCV), calcium, albumin, 25-hydroxyvitamin D (25-OH-D), PTH, and vitamin B12 with follow-up of 75...
November 8, 2017: Obesity Surgery
https://www.readbyqxmd.com/read/29115953/laparoscopic-sleeve-gastrectomy-improves-body-composition-and-alleviates-insulin-resistance-in-obesity-related-acanthosis-nigricans
#6
Yi Zhang, Cuiling Zhu, Xin Wen, Xingchun Wang, Liang Li, Sharvan Rampersad, Liesheng Lu, Donglei Zhou, Chunhua Qian, Ran Cui, Manna Zhang, Peng Yang, Shen Qu, Le Bu
BACKGROUND: Acanthosis nigricans (AN) has a close relationship with obesity. It is believed that obesity and AN have the common pathophysiological basis such as hyperinsulinism. This study is aimed to observe the effect of laparoscopic sleeve gastrectomy (LSG) on body composition and insulin resistance in Chinese obese patients with acanthosis nigricans. METHODS: A total of 37 obese patients who underwent LSG in our hospital were selected for analysis. They were divided into simple obesity (OB n = 14) and obesity with acanthosis nigricans (AN n = 23) group respectively...
November 7, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/29110650/group-based-developmental-bmi-trajectories-polycystic-ovary-syndrome-and-gestational-diabetes-a-community-based-longitudinal-study
#7
Nadira Sultana Kakoly, Arul Earnest, Lisa J Moran, Helena J Teede, Anju E Joham
BACKGROUND: Obesity is common in young women, increasing insulin resistance (IR) and worsening pregnancy complications, including gestational diabetes (GDM). Women with polycystic ovary syndrome (PCOS) are commonly obese, which aggravates the severity of PCOS clinical expression. Relationships between these common insulin-resistant conditions, however, remain unclear. METHODS: We conducted a secondary analysis of the Australian Longitudinal Study on Women's Health (ALSWH) database, including data from 8009 women aged 18-36 years across six surveys...
November 6, 2017: BMC Medicine
https://www.readbyqxmd.com/read/29110192/endoscopic-sleeve-gastroplasty-laparoscopic-sleeve-gastrectomy-and-laparoscopic-band-for-weight-loss-how-do-they-compare
#8
Aleksey A Novikov, Cheguevara Afaneh, Monica Saumoy, Viviana Parra, Alpana Shukla, Gregory F Dakin, Alfons Pomp, Enad Dawod, Shawn Shah, Louis J Aronne, Reem Z Sharaiha
BACKGROUND: Endoscopic sleeve gastroplasty (ESG) is a novel endobariatric procedure. Initial studies demonstrated an association of ESG with weight loss and improvement of obesity-related comorbidities. Our aim was to compare ESG to laparoscopic sleeve gastrectomy (LSG) and laparoscopic adjustable gastric banding (LAGB). METHODS: We included 278 obese (BMI > 30) patients who underwent ESG (n = 91), LSG (n = 120), or LAGB (n = 67) at our tertiary care academic center...
November 6, 2017: Journal of Gastrointestinal Surgery: Official Journal of the Society for Surgery of the Alimentary Tract
https://www.readbyqxmd.com/read/29099314/outcomes-in-super-obese-patients-undergoing-laparoscopic-sleeve-gastrectomy
#9
Vitish Singla, Sandeep Aggarwal, Harshit Garg, Lokesh Kashyap, Dilip R Shende, Samagra Agarwal
BACKGROUND: Super obese patients remain a challenge for management because of large liver size resulting in decreased work space and associated comorbidities. OBJECTIVES: To study outcomes in super obese patients undergoing Laparoscopic sleeve gastrectomy (LSG). METHODS: Retrospective data of 123 patients undergoing LSG from January 2008 to March 2015 were analyzed prospectively. RESULTS: Mean age and body mass index (BMI) of 123 patients (± 2 standard deviation [SD]) were 39...
November 3, 2017: Journal of Laparoendoscopic & Advanced Surgical Techniques. Part A
https://www.readbyqxmd.com/read/29095195/safety-of-revision-sleeve-gastrectomy-compared-to-roux-y-gastric-bypass-after-failed-gastric-banding-analysis-of-the-mbsaqip
#10
Michał R Janik, Tomasz G Rogula, Rami R Mustafa, Adel Alhaj Saleh, Leena Khaitan
OBJECTIVE: The aim of this study was to assess the safety of revisional surgery to laparoscopic sleeve gastrectomy (LSG) compared to laparoscopic Roux-Y gastric bypass (LRYGB) after failed laparoscopic adjustable gastric banding (LAGB). BACKGROUND: The number of reoperations after failed gastric banding rapidly increased in the United States during the last several years. A common approach is band removal with conversion to another weight loss procedure such as gastric bypass or sleeve gastrectomy in a single procedure...
October 31, 2017: Annals of Surgery
https://www.readbyqxmd.com/read/29083335/laparoscopic-subtotal-gastrectomy-for-the-treatment-of-advanced-gastric-cancer-a-comparison-with-open-procedure-at-the-beginning-of-the-learning-curve
#11
Federico Marchesi, Giuseppina De Sario, Stefano Cecchini, Francesca Tartamella, Matteo Riccò, Andrea Romboli
BACKGROUND: In the last decades, after some initial concern, laparoscopic subtotal gastrectomy (LSG) is gaining popularity also for the treatment of advanced gastric cancer (AGC). The aim of this study is to compare a single surgeon initial experience on LSG and open subtotal gastrectomy in terms of surgical safety and radicality, postoperative recovery and midterm oncological outcomes. METHODS: a case control study was conducted matching the first 13 LSG for AGC with 13 open procedures performed by the same surgeon...
October 23, 2017: Acta Bio-medica: Atenei Parmensis
https://www.readbyqxmd.com/read/29081506/opposite-changes-in-meteorin-like-and-oncostatin-m-levels-are-associated-with-metabolic-improvements-after-bariatric-surgery
#12
S Pellitero, I Piquer-Garcia, G Ferrer-Curriu, R Puig, E Martínez, P Moreno, J Tarascó, J Balibrea, C Lerin, M Puig-Domingo, F Villarroya, A Planavila, D Sánchez-Infantes
Bariatric surgery is currently the most effective therapy for type 2 diabetes. However, the mechanisms underlying its beneficial effects remain elusive. Here, we studied the effects of bariatric surgery on circulating meteorin-like (Metrnl) and oncostatin m (OSM) levels, two hormones intimately linked to energy homeostasis. Metrnl and OSM levels were assessed at baseline, 6, and 12 months after laparoscopic sleeve gastrectomy (LSG) in 25 patients with obesity, as well as in 33 normal-weight controls. At baseline, patients with obesity showed lower Metrnl and higher OSM levels compared to controls...
October 30, 2017: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/29080042/a-retrospective-2-year-follow-up-of-late-complications-treated-surgically-and-endoscopically-after-laparoscopic-roux-en-y-gastric-bypass-lrygb-and-laparoscopic-sleeve-gastrectomy-lsg-for-morbid-obesity
#13
Mervi Javanainen, Anne Penttilä, Harri Mustonen, Anne Juuti, Tom Scheinin, Marja Leivonen
BACKGROUND: The laparoscopic Roux-en-Y gastric bypass (LRYGB) has been the gold standard for bariatric surgery, but recently, the laparoscopic sleeve gastrectomy (LSG) has gained popularity. At present, limited data is available on the long-term complications of these two types of surgery. The aim of this retrospective study was to compare the 2-year data about late (more than 30 days after surgery) complications that were treated surgically or endoscopically after LRYGB and LSG operations in a large hospital area with a single patient database...
October 28, 2017: Obesity Surgery
https://www.readbyqxmd.com/read/29067941/acute-pancreatitis-as-an-unusual-early-post-operative-complication-following-laparoscopic-sleeve-gastrectomy
#14
Ankush Sarwal, Rajesh Khullar, Anil Sharma, Vandana Soni, Manish Baijal, Pradeep Chowbey
Laparoscopic sleeve gastrectomy (LSG) at present one of the most commonly performed surgical treatments for morbid obesity worldwide. There are some complications regarding this procedure in the literature. This report presents a patient who developed acute pancreatitis immediate post-LSG. Patient was referred to our institute on 10th post-operative day with a complaint of fever, nausea, abdominal pain and leucocytosis. A diagnostic laparoscopy showed pancreatitis. Post-operatively, the patient was managed on treatment line of acute pancreatitis and recovered well...
October 23, 2017: Journal of Minimal Access Surgery
https://www.readbyqxmd.com/read/29058236/postoperative-care-and-functional-recovery-after-laparoscopic-sleeve-gastrectomy-vs-laparoscopic-roux-en-y-gastric-bypass-among-patients-under-eras-protocol
#15
Piotr Major, Tomasz Stefura, Piotr Małczak, Michał Wysocki, Jan Witowski, Jan Kulawik, Mateusz Wierdak, Magdalena Pisarska, Michał Pędziwiatr, Andrzej Budzyński
BACKGROUND: The most commonly performed bariatric procedures are laparoscopic sleeve gastrectomy (LSG) and laparoscopic Roux-en-Y gastric bypass (LRYGB). There are major differences between LSG and LRYGB during postoperative period. Optimization of the postoperative care may be achieved by using enhanced recovery after surgery (ERAS) protocol, which allows earlier functional recovery. PURPOSE: The aim was to assess differences in the course of postoperative care conducted in accordance with ERAS protocol among patients after LSG and LRYGB...
October 23, 2017: Obesity Surgery
https://www.readbyqxmd.com/read/29051034/pre-operative-and-post-operative-changes-in-crp-and-other-biomarkers-sensitive-to-inflammatory-status-in-patients-with-severe-obesity-undergoing-laparoscopic-sleeve-gastrectomy
#16
Edward W Randell, Laurie K Twells, Deborah M Gregory, Kendra K Lester, Noriko Daneshtalab, Carla Dillon, David Pace, Chris Smith, Darrell Boone
INTRODUCTION: C-reactive protein (CRP) is often elevated in patients living with severe obesity (BMI≥35kg/m(2)). However, there is limited information on how CRP, and other inflammation responsive biomarkers, change in response to weight loss following laparoscopic sleeve gastrectomy (LSG). We studied how CRP, ferritin and albumin change following LSG surgery in relation to obesity, metabolic syndrome (MetS) ATPIII risk components and diabetes mellitus (DM). METHODS: Laboratory parameters (including CRP) were examined in 197 patients prior to LSG, and at 6, 12, 18 and 24months...
October 16, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/29043549/prevalence-and-predictors-of-gastroesophageal-reflux-disease-after-laparoscopic-sleeve-gastrectomy
#17
Saad Althuwaini, Fahad Bamehriz, Abdullah Aldohayan, Waleed Alshammari, Saleh Alhaidar, Mazen Alotaibi, Abdullah Alanazi, Hossam Alsahabi, Majid Abdularahman Almadi
BACKGROUND AND AIMS: Laparoscopic sleeve gastrectomy (LSG) might be associated with a new onset or worsening of gastroesophageal reflux disease (GERD). We aim to evaluate the prevalence of post-LSG GERD symptoms and its predictors. METHODS: We included patients who underwent primary LSG at a university hospital from 2009 to 2015. We used the GERD-Health-Related Quality of Life (GERD-HRQL) questionnaire and included questions regarding regurgitation to evaluate symptoms before and after LSG; each item was scored from 1 to 5 based on the symptom severity...
October 18, 2017: Obesity Surgery
https://www.readbyqxmd.com/read/29043548/comparison-of-short-term-effectiveness-and-postoperative-complications-laparoscopic-gastric-plication-vs-laparoscopic-sleeve-gastrectomy
#18
Mohammad Talebpour, Donya Sadid, Atieh Talebpour, Amirsina Sharifi, Farzad Vaghef Davari
INTRODUCTION: Bariatric surgeries are the only effective long-term treatment in obese patients. The innovation of laparoscopic gastric plication (LGP) raised some questions about its effectiveness compared to traditionally used techniques such as laparoscopic sleeve gastrectomy (LSG). We tried to answer some of these questions. MATERIALS AND METHODS: We investigated 70 patients in a randomized clinical trial (IRCT2013123012294N5) from 2012 to 2015. Thirty-five patients were randomly assigned to each LSG or LGP group, using sealed envelope method...
October 17, 2017: Obesity Surgery
https://www.readbyqxmd.com/read/29029355/over-sewing-of-staple-line-in-laparoscopic-sleeve-gastrectomy-initial-experience-of-a-comparative-study
#19
Giorgio Lisi, Roberto Rossini, Irene Gentile, Giacomo Ruffo
INTRODUCTION: The main drawback of laparoscopic sleeve gastrectomy (LSG) is the severity of postoperative complications. Staple line reinforcement (SLR) is strongly advocated. Recently, over-sewing has been proposed as a cost-effective and helpful method for reinforcing the staple line. The purpose of this study was to report our initial experience with LSG comparing over-sewing over the entire staple line with the upper-third staple line. MATERIALS AND METHODS: All obese patients seen at Negrar Sacro Cuore, Don Calabria Hospital were entered into our prospective database and were retrospectively evaluated...
October 12, 2017: Surgical Technology International
https://www.readbyqxmd.com/read/29019565/sleeve-gastrectomy-and-fundoplication-as-a-single-procedure-in-patients-with-obesity-and-gastroesophageal-reflux
#20
Juan Pablo Lasnibat, Italo Braghetto, Luis Gutierrez, Felipe Sanchez
BACKGROUND: Bariatric surgery in Chile has seen an exponential increase in recent years, especially in sleeve gastrectomy. Its use is currently discussed in patients suffering from gastroesophageal reflux disease. Different options have been considered for the management of these patients but up to now laparoscopic Roux-en-Y gastric bypass seems to be the best option. Sleeve gastrectomy plus concomitant fundoplication or hiatal hernia repair also has been suggested in patients having reflux or small hiatal hernia...
July 2017: Arquivos Brasileiros de Cirurgia Digestiva: ABCD, Brazilian Archives of Digestive Surgery
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