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https://www.readbyqxmd.com/read/29342297/an-exact-goodness-of-fit-test-based-on-the-occupancy-problems-to-study-zero-inflation-and-zero-deflation-in-biological-dosimetry-data
#1
Amanda Fernández-Fontelo, Pedro Puig, Elizabeth A Ainsbury, Manuel Higueras
The goal in biological dosimetry is to estimate the dose of radiation that a suspected irradiated individual has received. For that, the analysis of aberrations (most commonly dicentric chromosome aberrations) in scored cells is performed and dose response calibration curves are built. In whole body irradiation (WBI) with X- and gamma-rays, the number of aberrations in samples is properly described by the Poisson distribution, although in partial body irradiation (PBI) the excess of zeros provided by the non-irradiated cells leads, for instance, to the Zero-Inflated Poisson distribution...
January 12, 2018: Radiation Protection Dosimetry
https://www.readbyqxmd.com/read/29319401/age-dependent-differences-in-dna-damage-after-in-vitro-ct-exposure
#2
Maria Gomolka, Ursula Oestreicher, Ute Roessler, Daniel Samaga, David Endesfelder, Peter Lang, Klement Neumaier, Claus Belka, Markus Niemeyer, Marion Kiechle, Uwe Hasbargen, Christoph Hübener, Hans-Joachim Kirlum, Ulrike Kulka, Albert Rosenberger, Linda Walsh, Sarah Baatout, Ausra Kesminiene, Carita Lindholm
PURPOSE: Age dependent radiation sensitivity for DNA damage after in vitro blood exposure by computer tomography (CT) was investigated. MATERIALS AND METHODS: Radiation biomarkers (dicentrics and gammaH2AX) in blood samples of newborns, children under 5 years and adults after sham exposure (0 mGy), low dose (41 mGy) and high dose (978 mGy) in vitro CT exposure were analysed. RESULTS: Significantly higher levels of dicentric induction were found for the single and combined newborns/children group compared to adults, by a factor of 1...
January 10, 2018: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/29294545/-individual-dose-monitoring-and-health-status-of-radiation-workers-in-some-three-a-hospitals-of-zhejiang-province
#3
A F Ye, Z Q Xuan, Q Y Ni, X G Wang, S Y Gao
Objective: To investigate the individual irridiateddose levelandhealth statusofoccupational externalexposureamong radiationworkers inThreeA hospitals ofZhejiangprovince,andprovideevidence for occupationalhealthmanagement. Methods: 367 different typesof radiationworkerswereexperienced thedose monitoring and health examination from January toDecember, 2015, according to the requirements of"SpecificationsofIndividualMonitoring forOccupationalExternalExposure"and"Specifications forOccupational healthsurveillance forradiationworkers"...
October 20, 2017: Chinese Journal of Industrial Hygiene and Occupational Diseases
https://www.readbyqxmd.com/read/29286516/cases-of-the-staff-unaccounted-exposure-during-the-construction-of-the-protective-shell-for-a-new-confinement-at-the-chnpp-site
#4
L K Bezdrobna, L V Tarasenko, T V Tsyganok, V A Kurochkina, V O Sushko, L I Shvayko
OBJECTIVE: cytogenetic control of the staff radiation exposure level during the construction of the protective shell for a new confinement at the ChNPP site. MATERIALS AND METHODS: A cytogenetic examination was carried out for 32 staff persons from the contracting organ izations involved into construction of the protective shell for a new confinement. Additionally, for the goal of com parison, 28 persons who did not have professional contacts with the radiation factors were inspected...
December 2017: Problemy Radiat︠s︡iĭnoï Medyt︠s︡yny Ta Radiobiolohiï
https://www.readbyqxmd.com/read/29215325/dosikit-a-new-portable-immunoassay-for-fast-external-irradiation-biodosimetry
#5
Julie Bensimon Etzol, Marco Valente, Sandrine Altmeyer, Caroline Bettencourt, Samuel Bouvet, Guillaume Cosler, François Desangles, Michel Drouet, Fabrice Entine, Francis Hérodin, Flora Jourquin, Yannick Lecompte, Patrick Martigne, Xavier Michel, Jérôme Pateux, Nicolas Ugolin, Sylvie Chevillard
DosiKit is a new field-radiation biodosimetry immunoassay for rapid triage of individuals exposed to external total-body radiation. Here, we report on the validation of this immunoassay in human blood cell extracts 0.5 h after in vitro exposure to 137Cs gamma rays, using γ-H2AX analysis. First, calibration curves were established for five donors at doses ranging from 0 to 10 Gy and dose rates ranging from ∼0.8 to ∼3 Gy/min. The calibration curves, together with a γ-H2AX peptide scale, enabled the definition of inter-experimental correction factors...
December 7, 2017: Radiation Research
https://www.readbyqxmd.com/read/29196303/whole-chromosome-loss-and-associated-breakage-fusion-bridge-cycles-transform-mouse-tetraploid-cells
#6
Rozario Thomas, Daniel Henry Marks, Yvette Chin, Robert Benezra
Whole chromosome gains or losses (aneuploidy) are a hallmark of ~70% of human tumors. Modeling the consequences of aneuploidy has relied on perturbing spindle assembly checkpoint (SAC) components, but interpretations of these experiments are clouded by the multiple functions of these proteins. Here, we used a Cre recombinase-mediated chromosome loss strategy to individually delete mouse chromosomes 9, 10, 12, or 14 in tetraploid immortalized murine embryonic fibroblasts. This methodology also involves the generation of a dicentric chromosome intermediate, which subsequently undergoes a series of breakage-fusion-bridge (BFB) cycles...
December 1, 2017: EMBO Journal
https://www.readbyqxmd.com/read/29192617/the-effects-of-low-frequency-noise-on-rats-evidence-of-chromosomal-aberrations-in-the-bone-marrow-cells-and-the-release-of-low-molecular-weight-dna-in-the-blood-plasma
#7
Irina N Vasilyeva, Vladimir G Bespalov, Alexander L Semenov, Denis A Baranenko, Valery N Zinkin
OBJECTIVES: Evaluation of the effect of low-frequency noise (LFN) on the frequency of chromosomal aberrations in the bone marrow cells and on the content of low-molecular-weight DNA (lmwDNA) in the blood plasma of rats. MATERIALS AND METHODS: A total of 96 male Wistar rats were exposed to either single (17 min session) or multiple (17 min session repeated five times a week for 13 weeks) LFN, with the maximum range below 250 Hz and the sound pressure levels (SPLs) at 120 and 150 dB, respectively...
March 2017: Noise & Health
https://www.readbyqxmd.com/read/29093755/unique-amplification-of-bcr-abl1-gene-fusion-in-a-case-of-t-cell-acute-lymphoblastic-leukemia
#8
Rima Koka, Najeebah A Bade, Edward A Sausville, Yi Ning, Ying Zou
Background: ABL1 gene translocations can be seen in precursor T-acute lymphoblastic leukemia (T-ALL). The typical translocation partner is the NUP214 gene. BCR-ABL translocations are relatively rare in this entity. Furthermore, while there have been unique patterns of amplification noted among the NUP214-ABL fusion genes, there have been few such reports among cases with BCR-ABL fusion genes. Case presentation: Here we report a unique case of a 44-year old patient with T-ALL in which the blasts demonstrated a derivative chromosome 9 involving a 9;22 translocation and a dicentric Philadelphia chromosome 22 with a homogeneously staining region at the interface of the 9;22 translocation, leading to BCR-ABL1 gene amplification...
2017: Molecular Cytogenetics
https://www.readbyqxmd.com/read/29080427/a-comparison-of-estimates-of-doses-to-radiotherapy-patients-obtained-with-the-dicentric-chromosome-analysis-and-the-%C3%AE-h2ax-assay-relevance-to-radiation-triage
#9
Bhavani Manivannan, Thayalan Kuppusamy, Srinivasan Venkatesan, Venkatachalam Perumal
The γ-H2AX assay was investigated as an alternative to the time-consuming dicentric chromosome assay (DCA). Radiation doses to 25 radiotherapy patients were estimated in parallel by DCA and the γ-H2AX assay. The γ-H2AX assay yielded doses in line with the calculated equivalent whole body doses in 92% of the patients, whereas the success rate of DCA was only 76%. The result shows that the γ-H2AX assay can be effectively used as a rapid and more precise alternative to DCA.
October 16, 2017: Applied Radiation and Isotopes
https://www.readbyqxmd.com/read/29040682/dose-response-curves-for-analyzing-of-dicentric-chromosomes-and-chromosome-translocations-following-doses-of-1000-mgy-or-less-based-on-irradiated-peripheral-blood-samples-from-five-healthy-individuals
#10
Y Abe, Mitsuaki A Yoshida, Kurumi Fujioka, Yumiko Kurosu, Risa Ujiie, Aki Yanagi, Naohiro Tsuyama, Tomisato Miura, Toshiya Inaba, Kenji Kamiya, Akira Sakai
In terms of biological dosimetry at the time of radiation exposure, the dicentric chromosome (Dic) assay (DCA) is the gold standard for assessing for the acute phase and chromosome translocation (Tr) analysis is the gold standard for assessing the chronic phase. It is desirable to have individual dose-response curves (DRCs) for each laboratory because the analysis criteria differ between laboratories. We constructed the DRCs for radiation dose estimation (with three methods) using peripheral blood (PB) samples from five healthy individuals...
October 13, 2017: Journal of Radiation Research
https://www.readbyqxmd.com/read/29032586/cohesion-and-centromere-activity-are-required-for-histone-h3-phosphorylation-in-maize
#11
Yang Liu, Handong Su, Yalin Liu, Jing Zhang, Qianhua Dong, James A Birchler, Fangpu Han
Haspin-mediated histone H3-threonine 3 phosphorylation (H3T3ph) promotes proper deposition of Aurora B at the inner centromere to ensure faithful chromosome segregation in metazoans. However, the function of H3T3ph remains relatively unexplored in plants. Here, we show that in maize (Zea mays L.) mitotic cells, H3T3ph is concentrated at pericentromeric and centromeric regions. Additional weak H3T3ph signals occur between cohered sister chromatids at prometaphase. Immunostaining on dicentric chromosomes reveals that an inactive centromere cannot maintain H3T3ph at metaphase, indicating that a functional centromere is required for H3T3 phosphorylation...
October 15, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29026522/accurate-cytogenetic-biodosimetry-through-automated-dicentric-chromosome-curation-and-metaphase-cell-selection
#12
Jin Liu, Yanxin Li, Ruth Wilkins, Farrah Flegal, Joan H M Knoll, Peter K Rogan
Accurate digital image analysis of abnormal microscopic structures relies on high quality images and on minimizing the rates of false positive (FP) and negative objects in images. Cytogenetic biodosimetry detects dicentric chromosomes (DCs) that arise from exposure to ionizing radiation, and determines radiation dose received based on DC frequency. Improvements in automated DC recognition increase the accuracy of dose estimates by reclassifying FP DCs as monocentric chromosomes or chromosome fragments. We also present image segmentation methods to rank high quality digital metaphase images and eliminate suboptimal metaphase cells...
2017: F1000Research
https://www.readbyqxmd.com/read/28967092/dicentric-translocation-9-12-in-acute-lymphoblastic-leukemia-a-chromosomal-abnormality-with-an-excellent-prognosis
#13
Laura Illade, Victoria Fioravantti, Maitane Andion, Carmen Hernandez-Marques, Luis Madero, Alvaro Lassaletta
PURPOSE: Recurrent chromosomal abnormalities present in the leukemic cells of pediatric patients with acute lymphoblastic leukemia (ALL) often allow us to classify patients according to their prognosis, in order to establish further treatment. The dicentric translocation (9;12) consists of the rearrangement of the short arms of chromosomes 9 and 12 generating a dicentric chromosome (9;12). Patients with this alteration present a very good response to treatment and an excellent prognosis...
September 19, 2017: Tumori
https://www.readbyqxmd.com/read/28952879/exploring-the-link-between-radiation-exposure-and-multifocal-basal-cell-carcinomas-in-a-former-chernobyl-clean-up-worker-by-combining-different-molecular-biological-techniques
#14
Benjamin V Becker, Claus Richter, Reinhard Ullmann, Christina Beinke, Matthäus Majewski, Viktoria Exner, Guido Weisel, Michael Abend, Matthias Port
Thirty years after the Chernobyl nuclear power plant accident we report on a patient who was a clean-up worker, who subsequently developed multiple cutaneous basal cell carcinomas (BCCs). We used several methods to assess the biological long-term effects related to low-dose external and internal radiation exposure. Specifically, because BCC risk may be increased with ionizing radiation exposure, we endeavored to determine whether the multifocal BCCs were related to the patient's past clean-up work. We assessed cytogenetic changes using peripheral blood, and internal incorporation was measured with a whole-body counter...
November 2017: Radiation Research
https://www.readbyqxmd.com/read/28892030/expedited-radiation-biodosimetry-by-automated-dicentric-chromosome-identification-adci-and-dose-estimation
#15
Ben Shirley, Yanxin Li, Joan H M Knoll, Peter K Rogan
Biological radiation dose can be estimated from dicentric chromosome frequencies in metaphase cells. Performing these cytogenetic dicentric chromosome assays is traditionally a manual, labor-intensive process not well suited to handle the volume of samples which may require examination in the wake of a mass casualty event. Automated Dicentric Chromosome Identifier and Dose Estimator (ADCI) software automates this process by examining sets of metaphase images using machine learning-based image processing techniques...
September 4, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28863396/proximity-effects-in-chromosome-aberration-induction-by-low-let-ionizing-radiation
#16
John James Tello Cajiao, Mario Pietro Carante, Mario Antonio Bernal Rodriguez, Francesca Ballarini
Although chromosome aberrations are known to derive from distance-dependent mis-rejoining of chromosome fragments, evaluating whether a certain model describes such "proximity effects" better than another one is complicated by the fact that different approaches have often been tested under different conditions. Herein, a biophysical model ("BIANCA", i.e. BIophysical ANalysis of Cell death and chromosome Aberrations) was upgraded, implementing explicit chromosome-arm domains and two new models for the dependence of the rejoining probability on the fragment initial distance, r...
October 2017: DNA Repair
https://www.readbyqxmd.com/read/28843160/comparison-of-dicentric-dose-response-curves-of-6mv-linac-x-rays-and-60-co-%C3%AE-rays-for-biodosimetry-application
#17
A Arul Anantha Kumar, D Bakkiam, Swetha Sonwani, R Seenisamy, K Sivasubramanian, B Venkatraman
In vitro Dicentric (DC) dose response curves of 6MV X-rays (3Gy/min) and (60)Co γ-rays (0.63Gy/min) were generated and compared (intra-laboratory) to understand their similarities and differences. Human peripheral blood samples exposed to ten different doses (0, 0.05, 0.1, 0.25, 0.5, 1, 2, 3, 4 and 5Gy) of 6MV X-rays and (60)Co γ-rays were subjected to Dicentric Chromosome Assay (DCA) and dicentrics, excess acentric fragments (AF) and chromatid breaks (CB) were scored. Totally about 31,553 metaphase spreads were scored for the purpose...
November 2017: Applied Radiation and Isotopes
https://www.readbyqxmd.com/read/28840240/centromere-silencing-mechanisms
#18
Shannon M McNulty, Beth A Sullivan
Centromere function is essential for genome stability and chromosome inheritance. Typically, each chromosome has a single locus that consistently serves as the site of centromere formation and kinetochore assembly. Decades of research have defined the DNA sequence and protein components of functional centromeres, and the interdependencies of specific protein complexes for proper centromere assembly. Less is known about how centromeres are disassembled or functionally silenced. Centromere silencing, or inactivation, is particularly relevant in the cases of dicentric chromosomes that occur via genome rearrangements that place two centromeres on the same chromosome...
2017: Progress in Molecular and Subcellular Biology
https://www.readbyqxmd.com/read/28836280/molecular-characterisation-of-a-case-of-dicentric-y-presented-as-nonobstructive-azoospermia-with-testicular-early-maturation-arrest
#19
P Kumar, M Jain, A K Kalsi, A Halder
The dicentric Y chromosome is the most common cytogenetically visible structural abnormality of Y chromosome. The sites of break and fusion of dicentric Y are variable, but break and fusion at Yq12 (proximal to the pseudoautosomal region 2/PAR 2) is very rare. Dicentric Y chromosome is unstable during cell division and likely to generate chromosomal mosaicism. Here, we report a case of infertile male with nonmosaic 46,XY where chromosome Y was dicentric with break and fusion at Yq12 (proximal to PAR 2). Clinical presentation of the case was nonobstructive azoospermia due to early maturation arrest at the primary spermatocyte stage...
August 24, 2017: Andrologia
https://www.readbyqxmd.com/read/28607452/transmission-of-induced-chromosomal-aberrations-through-successive-mitotic-divisions-in-human-lymphocytes-after-in-vitro-and-%C3%AF-in%C3%AF-vivo-radiation
#20
Akram Kaddour, Bruno Colicchio, Diane Buron, Elie El Maalouf, Eric Laplagne, Claire Borie, Michelle Ricoul, Aude Lenain, William M Hempel, Luc Morat, Mustafa Al Jawhari, Corina Cuceu, Leonhard Heidingsfelder, Eric Jeandidier, Georges Deschênes, Alain Dieterlen, Michèle El May, Theodore Girinsky, Annelise Bennaceur-Griscelli, Patrice Carde, Laure Sabatier, Radhia M'kacher
The mechanisms behind the transmission of chromosomal aberrations (CA) remain unclear, despite a large body of work and major technological advances in chromosome identification. We reevaluated the transmission of CA to second- and third-division cells by telomere and centromere (TC) staining followed by M-FISH. We scored CA in lymphocytes of healthy donors after in vitro irradiation and those of cancer patients treated by radiation therapy more than 12 years before. Our data demonstrate, for the first time, that dicentric chromosomes (DCs) decreased by approximately 50% per division...
June 12, 2017: Scientific Reports
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