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https://www.readbyqxmd.com/read/29774413/alterations-in-histone-acetylation-following-exposure-to-60-co-%C3%AE-rays-and-their-relationship-with-chromosome-damage-in-human-lymphoblastoid-cells
#1
Xue-Lei Tian, Xue Lu, Jiang-Bin Feng, Tian-Jing Cai, Shuang Li, Mei Tian, Qing-Jie Liu
Chromosome damage is related to DNA damage and erroneous repair. It can cause cell dysfunction and ultimately induce carcinogenesis. Histone acetylation is crucial for regulating chromatin structure and DNA damage repair. Ionizing radiation (IR) can alter histone acetylation. However, variations in histone acetylation in response to IR exposure and the relationship between histone acetylation and IR-induced chromosome damage remains unclear. Hence, this study investigated the variation in the total acetylation levels of H3 and H4 in human lymphocytes exposed to 0-2 Gy 60 Co γ-rays...
May 17, 2018: Radiation and Environmental Biophysics
https://www.readbyqxmd.com/read/29624483/analysis-of-radiation-induced-chromosomal-aberrations-on-a-cell-by-cell-basis-after-alpha-particle-microbeam-irradiation-experimental-data-and-simulations
#2
Antonella Testa, Francesca Ballarini, Ulrich Giesen, Octávia Monteiro Gil, Mario P Carante, John Tello, Frank Langner, Hans Rabus, Valentina Palma, Massimo Pinto, Clarice Patrono
There is a continued need for further clarification of various aspects of radiation-induced chromosomal aberration, including its correlation with radiation track structure. As part of the EMRP joint research project, Biologically Weighted Quantities in Radiotherapy (BioQuaRT), we performed experimental and theoretical analyses on chromosomal aberrations in Chinese hamster ovary cells (CHO-K1) exposed to α particles with final energies of 5.5 and 17.8 MeV (absorbed doses: ∼2.3 Gy and ∼1.9 Gy, respectively), which were generated by the microbeam at the Physikalisch-Technische Bundesanstalt (PTB) in Braunschweig, Germany...
April 6, 2018: Radiation Research
https://www.readbyqxmd.com/read/29613823/analysis-of-gene-expression-changes-in-pha-m-stimulated-lymphocytes-unraveling-pha-activity-as-prerequisite-for-dicentric-chromosome-analysis
#3
C Beinke, M Port, R Ullmann, K Gilbertz, M Majewski, M Abend
Dicentric chromosome analysis (DCA) is the gold standard for individual radiation dose assessment. However, DCA is limited by the time-consuming phytohemagglutinin (PHA)-mediated lymphocyte activation. In this study using human peripheral blood lymphocytes, we investigated PHA-associated whole genome gene expression changes to elucidate this process and sought to identify suitable gene targets as a means of meeting our long-term objective of accelerating cell cycle kinetics to reduce DCA culture time. Human peripheral whole blood from three healthy donors was separately cultured in RPMI/FCS/antibiotics with BrdU and PHA-M...
April 3, 2018: Radiation Research
https://www.readbyqxmd.com/read/29589589/chromosome-aberrations-in-workers-occupationally-exposed-to-tritium
#4
E Janet Tawn, Gillian B Curwen, Anthony E Riddell
This paper reports the findings of an historical chromosome analysis for unstable aberrations, undertaken on 34 nuclear workers with monitored exposure to tritium. The mean recorded β-particle dose from tritium was 9.33 mGy (range 0.25-79.71 mGy) and the mean occupational dose from external, mainly γ-ray, irradiation was 1.94 mGy (range 0.00-7.71 mGy). The dicentric frequency of 1.91 ± 0.53 × 10-3 per cell was significantly raised, in comparison with that of 0.61 ± 0.30 × 10-3 per cell for a group of 66 comparable worker controls unexposed to occupational radiation...
March 28, 2018: Journal of Radiological Protection: Official Journal of the Society for Radiological Protection
https://www.readbyqxmd.com/read/29574397/high-frequency-of-dicentric-chromosomes-detected-by-multi-centromeric-fish-in-patients-with-acute-myeloid-leukemia-and-complex-karyotype
#5
Iveta Sarova, Jana Brezinova, Zuzana Zemanova, Sarka Ransdorfova, Karla Svobodova, Silvia Izakova, Lenka Pavlistova, Libuse Lizcova, Adela Berkova, Karolina Skipalova, Lucie Hodanova, Cyril Salek, Anna Jonasova, Kyra Michalova
Dicentric chromosomes (DCs) are considered markers of cancer in various malignancies. However, they can be overlooked when conventional analysis or multicolor fluorescence in situ hybridization (mFISH) is used to detect complex karyotypes. We analyzed the karyotypes of 114 patients with acute myeloid leukemia (AML) and complex karyotypes and verified the presence of monosomies by FISH using multi-centromeric probes. Monosomy was detected in 63% of patients by G-banding/mFISH and confirmed in 55% of patients by centromeric FISH...
March 19, 2018: Leukemia Research
https://www.readbyqxmd.com/read/29564828/genes-proteins-and-biological-pathways-preventing-chromothripsis
#6
Martin Poot
The highly complex structural genome variations chromothripsis, chromoanasynthesis, and chromoplexy are subsumed under the term chromoanagenesis, which means chromosome rebirth. Precipitated by numerous DNA double-strand breaks, they differ in number of and distances between breakpoints, associated copy number variations, order and orientation of segments, and flanking sequences at joining points. Results from patients with the autosomal dominant cancer susceptibility disorder Li-Fraumeni syndrome implicated somatic TP53 mutations in chromothripsis...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29555061/frequency-of-chromosome-aberrations-among-adult-male-individuals-from-high-and-normal-level-natural-radiation-areas-of-kerala-in-the-southwest-coast-of-india
#7
Karuppasamy C V, Ramachandran E N, Anil Kumar V, Vivek Kumar P R, Koya P K M, Jaikrishan G, Birajalaxmi Das
Chromosome aberration analysis was carried out in peripheral blood lymphocytes of adult male individuals from normal level natural radiation areas (NLNRA, ≤1.5 mGy/year, N = 27) and high level natural radiation areas (HLNRA, >1.5mGy/year, N = 70) of Kerala coast in southwest India. The mean age of individuals from NLNRA and HLNRA was 40.9 ± 9.4 and 43.7 ± 12.4 years, respectively, with an overall mean of 42.9 ± 11.6 (range: 18-80). Whole-blood cultures were set up and about 260 metaphases were scored per individual...
April 2018: Mutation Research
https://www.readbyqxmd.com/read/29494834/proximity-effects-in-chromosome-aberration-induction-dependence-on-radiation-quality-cell-type-and-dose
#8
John James Tello Cajiao, Mario Pietro Carante, Mario Antonio Bernal Rodriguez, Francesca Ballarini
It is widely accepted that, in chromosome-aberration induction, the (mis-)rejoining probability of two chromosome fragments depends on their initial distance, r. However, several aspects of these "proximity effects" need to be clarified, also considering that they can vary with radiation quality, cell type and dose. A previous work performed by the BIANCA (BIophysical ANalysis of Cell death and chromosome Aberrations) biophysical model has suggested that, in human lymphocytes and fibroblasts exposed to low-LET radiation, an exponential function of the form exp(-r/r0 ), which is consistent with free-end (confined) diffusion, describes proximity effects better than a Gaussian function...
April 2018: DNA Repair
https://www.readbyqxmd.com/read/29494324/correlation-of-radiation-dose-estimates-by-dic-with-the-metrepol-hematological-classes-of-disease-severity
#9
M Port, B Pieper, H D Dörr, A Hübsch, M Majewski, M Abend
The degree of severity of hematologic acute radiation syndrome (HARS) may vary across the range of radiation doses, such that dose alone may be a less reliable predictor of clinical course. We sought to elucidate the relationship between absorbed dose and risk of clinically relevant HARS in humans. We used the database SEARCH (System for Evaluation and Archiving of Radiation Accidents based on Case Histories), which contains the histories of radiation accident victims. From 153 cases we extracted data on dose estimates using the dicentric assay to measure individual biological dosimetry...
May 2018: Radiation Research
https://www.readbyqxmd.com/read/29486464/chromosome-rearrangements-caused-by-double-monosomy-in-wheat-barley-group-7-substitution-lines
#10
Tatiana V Danilova, Bernd Friebe, Bikram S Gill, Jesse Poland, Eric Jackson
Interspecific or introgressive hybridization is one of the driving forces in plant speciation, producing allopolyploids or diploids with rearranged genomes. The process of karyotype reshaping following homoploid interspecific hybridization has not been studied experimentally. Interspecific hybridization is widely used in plant breeding to increase genetic diversity and introgress new traits. Numerous introgression stocks were developed for hexaploid wheat Triticum aestivum L. (2n = 6x = 42, genome AABBDD). Double monosomic lines, containing one alien chromosome from the tertiary gene pool of wheat and one homoeologous wheat chromosome, represent a simplified model for studying chromosome rearrangements caused by interspecific hybridization...
2018: Cytogenetic and Genome Research
https://www.readbyqxmd.com/read/29474504/the-first-in-vivo-multiparametric-comparison-of-different-radiation-exposure-biomarkers-in-human-blood
#11
Ales Tichy, Sylwia Kabacik, Grainne O'Brien, Jaroslav Pejchal, Zuzana Sinkorova, Adela Kmochova, Igor Sirak, Andrea Malkova, Caterina Gomila Beltran, Juan Ramon Gonzalez, Jakub Grepl, Matthaeus Majewski, Elizabeth Ainsbury, Lenka Zarybnicka, Jana Vachelova, Alzbeta Zavrelova, Marie Davidkova, Marketa Markova Stastna, Michael Abend, Eileen Pernot, Elisabeth Cardis, Christophe Badie
The increasing risk of acute large-scale radiological/nuclear exposures of population underlines the necessity of developing new, rapid and high throughput biodosimetric tools for estimation of received dose and initial triage. We aimed to compare the induction and persistence of different radiation exposure biomarkers in human peripheral blood in vivo. Blood samples of patients with indicated radiotherapy (RT) undergoing partial body irradiation (PBI) were obtained soon before the first treatment and then after 24 h, 48 h, and 5 weeks; i...
2018: PloS One
https://www.readbyqxmd.com/read/29397502/effects-of-spirulina-platensis-on-dna-damage-and-chromosomal-aberration-against-cadmium-chloride-induced-genotoxicity-in-rats
#12
Fayza M Aly, Ahmed M Kotb, Seddik Hammad
Todays, bioactive compounds extracted from Spirulina platensis have been intensively studied for their therapeutical values. Therefore, in the present study, we aimed to evaluate the effects of S. platensis extract on DNA damage and chromosomal aberrations induced by cadmium in rats. Four groups of male albino rats (n = 7 rats) were used. The first group served as a control group and received distilled water. The second group was exposed intraperitoneally to cadmium chloride (CdCl2 ) (3.5 mg/kg body weight dissolved in 2 ml distilled water)...
April 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29378067/associations-of-polymorphisms-in-the-cytokine-genes-il1%C3%AE-rs16944-il6-rs1800795-il12b-rs3212227-and-growth-factor-vegfa-rs2010963-with-anthracosilicosis-in-coal-miners-in-russia-and-related-genotoxic-effects
#13
Valentin P Volobaev, Aleksey V Larionov, Ekaterina E Kalyuzhnaya, Ekaterina S Serdyukova, Svetlana Yakovleva, Vladimir G Druzhinin, Olga O Babich, Elena G Hill, Victor A Semenihin, Nikolay I Panev, Varvara I Minina, Saravana Devi Sivanesan, Pravin Naoghare, Juliana da Silva, Gustavo R M Barcelos, Alexander Y Prosekov
Anthracosilicosis (AS), a prevalent form of pneumoconiosis among coal miners, results from the accumulation of carbon and silica in the lungs from inhaled coal dust. This study investigated genotoxic effects and certain cytokine genes polymorphic variants in Russian coal miners with АS. Peripheral leukocytes were sampled from 129 patients with AS confirmed by X-ray and tissue biopsy and from 164 asymptomatic coal miners. Four single-nucleotide polymorphisms were genotyped in the extracted DNA samples: IL1β T-511C (rs16944), IL6 C-174G (rs1800795), IL12b A1188C (rs3212227) and VEGFA C634G (rs2010963)...
April 13, 2018: Mutagenesis
https://www.readbyqxmd.com/read/29363380/twenty-years-of-fish-based-translocation-analysis-for-retrospective-ionizing-radiation-biodosimetry
#14
Eric Grégoire, Laurence Roy, Valérie Buard, Martine Delbos, Valérie Durand, Cécile Martin-Bodiot, Pascale Voisin, Irène Sorokine-Durm, Aurélie Vaurijoux, Philippe Voisin, Céline Baldeyron, Joan Francesc Barquinero
PURPOSE: The fluorescent in situ hybridization (FISH) technique, which easily detects reciprocal translocations, is currently used to estimate doses in retrospective biological dosimetry, after suspected accidental overexposure to ionizing radiation (IR). This study of 42 cases aimed to verify the appropriateness of this assay for radiation dose reconstruction, compared to the dicentric assay, and to evaluate other limitations. MATERIAL AND METHODS: We labeled chromosomes 2, 4, and 12 by 3-color FISH painting to detect translocations on lymphocytes of patients with suspected past IR overexposure...
March 2018: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/29342297/an-exact-goodness-of-fit-test-based-on-the-occupancy-problems-to-study-zero-inflation-and-zero-deflation-in-biological-dosimetry-data
#15
Amanda Fernández-Fontelo, Pedro Puig, Elizabeth A Ainsbury, Manuel Higueras
The goal in biological dosimetry is to estimate the dose of radiation that a suspected irradiated individual has received. For that, the analysis of aberrations (most commonly dicentric chromosome aberrations) in scored cells is performed and dose response calibration curves are built. In whole body irradiation (WBI) with X- and gamma-rays, the number of aberrations in samples is properly described by the Poisson distribution, although in partial body irradiation (PBI) the excess of zeros provided by the non-irradiated cells leads, for instance, to the Zero-Inflated Poisson distribution...
January 12, 2018: Radiation Protection Dosimetry
https://www.readbyqxmd.com/read/29319401/age-dependent-differences-in-dna-damage-after-in-vitro-ct-exposure
#16
Maria Gomolka, Ursula Oestreicher, Ute Rößler, Daniel Samaga, David Endesfelder, Peter Lang, Klement Neumaier, Claus Belka, Markus Niemeyer, Marion Kiechle, Uwe Hasbargen, Christoph Hübener, Hans-Joachim Kirlum, Ulrike Kulka, Albert Rosenberger, Linda Walsh, Sarah Baatout, Ausrele Kesminiene, Carita Lindholm
PURPOSE: Age dependent radiation sensitivity for DNA damage after in vitro blood exposure by computer tomography (CT) was investigated. MATERIALS AND METHODS: Radiation biomarkers (dicentrics and gammaH2AX) in blood samples of newborns, children under five years and adults after sham exposure (0 mGy), low-dose (41 mGy) and high-dose (978 mGy) in vitro CT exposure were analyzed. RESULTS: Significantly higher levels of dicentric induction were found for the single and combined newborns/children group compared to adults, by a factor of 1...
March 2018: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/29294545/-individual-dose-monitoring-and-health-status-of-radiation-workers-in-some-three-a-hospitals-of-zhejiang-province
#17
A F Ye, Z Q Xuan, Q Y Ni, X G Wang, S Y Gao
Objective: To investigate the individual irridiateddose levelandhealth statusofoccupational externalexposureamong radiationworkers inThreeA hospitals ofZhejiangprovince,andprovideevidence for occupationalhealthmanagement. Methods: 367 different typesof radiationworkerswereexperienced thedose monitoring and health examination from January toDecember, 2015, according to the requirements of"SpecificationsofIndividualMonitoring forOccupationalExternalExposure"and"Specifications forOccupational healthsurveillance forradiationworkers"...
October 20, 2017: Chinese Journal of Industrial Hygiene and Occupational Diseases
https://www.readbyqxmd.com/read/29286516/cases-of-the-staff-unaccounted-exposure-during-the-construction-of-the-protective-shell-for-a-new-confinement-at-the-chnpp-site
#18
L K Bezdrobna, L V Tarasenko, T V Tsyganok, V A Kurochkina, V O Sushko, L I Shvayko
OBJECTIVE: cytogenetic control of the staff radiation exposure level during the construction of the protective shell for a new confinement at the ChNPP site. MATERIALS AND METHODS: A cytogenetic examination was carried out for 32 staff persons from the contracting organ izations involved into construction of the protective shell for a new confinement. Additionally, for the goal of com parison, 28 persons who did not have professional contacts with the radiation factors were inspected...
December 2017: Problemy Radiat︠s︡iĭnoï Medyt︠s︡yny Ta Radiobiolohiï
https://www.readbyqxmd.com/read/29215325/dosikit-a-new-portable-immunoassay-for-fast-external-irradiation-biodosimetry
#19
Julie Bensimon Etzol, Marco Valente, Sandrine Altmeyer, Caroline Bettencourt, Samuel Bouvet, Guillaume Cosler, François Desangles, Michel Drouet, Fabrice Entine, Francis Hérodin, Flora Jourquin, Yannick Lecompte, Patrick Martigne, Xavier Michel, Jérôme Pateux, Nicolas Ugolin, Sylvie Chevillard
DosiKit is a new field-radiation biodosimetry immunoassay for rapid triage of individuals exposed to external total-body radiation. Here, we report on the validation of this immunoassay in human blood cell extracts 0.5 h after in vitro exposure to137 Cs gamma rays, using γ-H2AX analysis. First, calibration curves were established for five donors at doses ranging from 0 to 10 Gy and dose rates ranging from ∼0.8 to ∼3 Gy/min. The calibration curves, together with a γ-H2AX peptide scale, enabled the definition of inter-experimental correction factors...
December 7, 2017: Radiation Research
https://www.readbyqxmd.com/read/29196303/whole-chromosome-loss-and-associated-breakage-fusion-bridge-cycles-transform-mouse-tetraploid-cells
#20
Rozario Thomas, Daniel Henry Marks, Yvette Chin, Robert Benezra
Whole chromosome gains or losses (aneuploidy) are a hallmark of ~70% of human tumors. Modeling the consequences of aneuploidy has relied on perturbing spindle assembly checkpoint (SAC) components, but interpretations of these experiments are clouded by the multiple functions of these proteins. Here, we used a Cre recombinase-mediated chromosome loss strategy to individually delete mouse chromosomes 9, 10, 12, or 14 in tetraploid immortalized murine embryonic fibroblasts. This methodology also involves the generation of a dicentric chromosome intermediate, which subsequently undergoes a series of breakage-fusion-bridge (BFB) cycles...
January 17, 2018: EMBO Journal
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