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https://www.readbyqxmd.com/read/28945007/nonmineralized-and-mineralized-collagen-scaffolds-induce-differential-osteogenic-signaling-pathways-in-human-mesenchymal-stem-cells
#1
Qi Zhou, Xiaoyan Ren, David Bischoff, Daniel W Weisgerber, Dean T Yamaguchi, Timothy A Miller, Brendan A C Harley, Justine C Lee
The instructive capabilities of extracellular matrix components in progenitor cell differentiation have recently generated significant interest in the development of bioinspired materials for regenerative applications. Previously, a correlation was described between the osteogenic capabilities of nanoparticulate mineralized collagen glycosaminoglycan scaffolds (MC-GAG) and an autogenous activation of small mothers against decapentaplegic ( Smad1/5) in the canonical bone morphogenetic protein receptor (BMPR) pathway with a diminished extracellular signal regulated kinase 1/2 (ERK1/2) activation when compared to nonmineralized collagen glycosaminoglycan scaffolds (Col-GAG)...
September 25, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28944964/wnt-%C3%AE-catenin-and-erk-pathway-activation-a-possible-mechanism-of-photobiomodulation-therapy-with-light-emitting-diodes-that-regulate-the-proliferation-of-human-outer-root-sheath-cells
#2
Jung E Kim, Young J Woo, Ki M Sohn, Kwan H Jeong, Hoon Kang
BACKGROUND: Outer root sheath cells (ORSCs) play important roles in maintaining hair follicle structure and provide support for the bulge area. The hair growth promoting effects of photobiomodulation therapy (PBMT) have been reported, but the mechanisms for this in human ORCs (hORSCs) have rarely been studied. OBJECTIVE: The aim of this study was to investigate the effect of various wavelengths of light-emitting diode (LED) irradiation on human ORSCs (hORSCs). METHODS: LED irradiation effects on hORSC proliferation and migration were examined with MTT assay, BrdU incorporation assay and migration assays...
September 25, 2017: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/28944919/proprotein-convertase-subtilisin-kexin-type-6-promotes-in%C3%A2-vitro-proliferation-migration-and-inflammatory-cytokine-secretion-of-synovial-fibroblast%C3%A2-like-cells-from-rheumatoid-arthritis-via-nuclear%C3%A2-%C3%AE%C2%BAb-signal-transducer-and-activator-of-transcription-3-and
#3
Huiyu Jiang, Lin Wang, Feifei Wang, Jihong Pan
Our previous studies demonstrated that expression of proprotein convertase subtilisin/kexin type 6 (PCSK6) is greatly enhanced in rheumatoid arthritis fibroblast‑like synoviocytes (RASFs), and that PCSK6 inhibition decreases cell proliferation, migration and invasion. The present study aimed to investigate the functional role of PCSK6 in the hyperplasia of RASFs. Cultured RASFs from RA patients were stimulated with recombinant human (rh)PCSK6. Subsequent changes in proliferation, invasion, migration and the secretion of inflammatory cytokines were measured in vitro using MTT, wound healing and Transwell assays, and ELISA...
September 25, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944886/sulforaphane-induces-p53%C3%A2-deficient-sw480-cell-apoptosis-via-the-ros%C3%A2-mapk-signaling-pathway
#4
Hai Lan, Hongyin Yuan, Congyao Lin
Sulforaphane (SFN) has been revealed to inhibit the growth and induce apoptosis of cancer cells. However, the detailed anticancer effects of SFN on p53‑deficient colon cancer cells has yet to be clearly elucidated. The present study employed p53‑deficient SW480 cells to establish an SFN‑induced in vitro model of apoptosis. The critical events leading to apoptosis were then evaluated in SFN‑treated p53‑deficient SW480 cells, by performing an MTT assay, flow cytometry, western blotting and ELISA. The results demonstrated that SFN at concentrations of 5, 10, 15 and 20 µM induced mitochondria‑associated cell apoptosis, which was further confirmed by disruption of the mitochondrial membrane potential, an increase in the Bax/Bcl‑2 ratio, as well as activation of caspase‑3, ‑7 and ‑9...
September 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944877/sparcl1-suppresses-cell-migration-and-invasion-in-renal-cell-carcinoma
#5
Hui Ye, Wei-Gang Wang, Jun Cao, Xi-Chun Hu
Previous studies have shown that the human SPARC‑like 1 (SPARCL1) is crucial for human cancer migration and invasion. In the present study, the expression, biological function and possible molecular regulatory mechanisms of SPARCL1 were investigated in human renal cell carcinoma (RCC). The protein expression of SPARCL1 in cells was evaluated using western blot analysis and immunohistochemical staining in the tissue microarray. The effects of SPARCL1 on the biological behaviors of RCC cells were assessed using in vitro assays...
September 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944873/coxsackieviruses-b3-infection-of-myocardial-microvascular-endothelial-cells-activates-fractalkine-via-the-erk1-2-signaling-pathway
#6
Jili Wen, Congxin Huang
Infections by pathogens may lead to cardiovascular diseases, including acute/chronic myocarditis. (Coxsackieviruses B3) CVB3 is considered to be the most common causative agent in m‑yocarditis, which can lead to dilated cardiomyopathy. The present study aimed to investigate the mechanism of CVB3‑infected myocardial microvascular endothelial cells. The CVB3 infection was detected by 50% tissue culture infective dose (TCID50). The role of fractalkine (FKN) in the infection was detected using western blotting and RNA interference...
September 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944867/succinate-promotes-skeletal-muscle-protein-synthesis-via-erk1-2-signaling-pathway
#7
Yexian Yuan, Yaqiong Xu, Jingren Xu, Bingqing Liang, Xingcai Cai, Canjun Zhu, Lina Wang, Songbo Wang, Xiaotong Zhu, Ping Gao, Xiuqi Wang, Yongliang Zhang, Qingyan Jiang, Gang Shu
It is well known that endurance training is effective to attenuate skeletal muscle atrophy. Succinate is a typical TCA metabolite, of which exercise could dramatically increase the content. The present study aimed to investigate the effect of succinate on protein synthesis in skeletal muscle, and try to delineate the underlying mechanism. The in vitro study revealed that succinate dose‑dependently increased protein synthesis in C2C12 myotube along with the enhancement of phosphorylation levels of AKT Serine/Threonine Kinase 1(Akt), mammalian target of rapamycin, S6, eukaryotic translation initiation factor 4E, 4E binding protein 1 and forkhead box O (FoxO) 3a...
September 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944860/polyaniline-promotes-peripheral-nerve-regeneration-by-enhancement-of-the-brain%C3%A2-derived-neurotrophic-factor-and-ciliary-neurotrophic-factor-expression-and-activation-of-the-erk1-2-mapk-signaling-pathway
#8
Lin Fan, Yan Xiong, Zhen Fu, Dingfeng Xu, Lei Wang, Yong Chen, Haoyang Xia, Na Peng, Shaojun Ye, Yanfeng Wang, Lina Zhang, Qifa Ye
A previous study has demonstrated a progression in the nerve regeneration by polyaniline/cellulose (PANI/RC), although the underlying mechanism was not elucidated. In the present study, regenerated nerves were investigated, using histological techniques, functional assays and western blot analysis. The triceps surae muscle weight ratio percentages of the sham, regenerated cellulose (RC) and the PANI/RC groups were 38.88±4.76 and 76.32±7.11%, respectively. The thickness of the myelin sheath for the aforementioned groups were as follows: 1...
September 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944838/effect-of-antioxidant-of-bamboo-leaves-on-gene-expression-associated-with-mouse-embryonic-fibroblast-reproduction-and-embryonic-development
#9
Feng Yu, Xiaowei Qian, Zhanghui Zeng, Xiaoli Zhao, Rong Hou, Zhihe Zhang, Hongwu Bian, Ning Han, Junhui Wang, Muyuan Zhu
Antioxidant of bamboo leaves (AOB) was certified to be a natural antioxidant by the Chinese Ministry of Health in 2003. However, the effects of AOB on animal reproductive and developmental functions remain unclear. The present study aimed to investigate the effects of different concentrations of AOB on mouse embryonic fibroblast (MEF) cells, and to examine the underlying molecular mechanism through which AOB affects the proliferation and apoptosis of MEFs. MEFs prepared from individual embryos were treated with various dosages of AOB...
September 18, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28943410/loss-of-reelin-protects-mice-against-arterial-thrombosis-by-impairing-integrin-activation-and-thrombus-formation-under-high-shear-conditions
#10
Nina Sarah Gowert, Irena Krüger, Meike Klier, Lili Donner, Friederike Kipkeew, Michael Gliem, Nicholas J Bradshaw, David Lutz, Sabrina Köber, Harald Langer, Sebastian Jander, Kerstin Jurk, Michael Frotscher, Carsten Korth, Hans H Bock, Margitta Elvers
Reelin is a secreted glycoprotein and essential for brain development and plasticity. Recent studies provide evidence that Reelin modifies platelet actin cytoskeletal dynamics. In this study we sought to dissect the contribution of Reelin in arterial thrombus formation. Here we analyzed the impact of Reelin in arterial thrombosis ex vivo and in vivo using Reelin deficient (reeler) and wildtype mice. We found that Reelin is secreted upon platelet activation and mediates signaling via glycoprotein (GP)Ib, the amyloid precursor protein (APP) and apolipoprotein E receptor 2 (ApoER2) to induce activation of Akt, extracellular signal-regulated kinase (Erk), SYK and Phospholipase Cγ2...
September 21, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28943105/a549-cells-as-a-model-to-study-endogenous-lpa1-receptor-signaling-and-regulation
#11
Gabriel Carmona-Rosas, Marco A Alfonzo-Méndez, David A Hernández-Espinosa, M Teresa Romero-Ávila, J Adolfo García-Sáinz
Lysophosphatidic acid (LPA) modulates the function of many organs, including the lung. A549 is a lung carcinoma-derived cell line, frequently used as a model for type II pneumocytes. Here we show that these cells expressed messenger RNA coding for LPA1-3 receptors with the following order of abundance: LPA1 > LPA2 > LPA3 and that LPA was able to increase intracellular calcium, extracellular signal-regulated kinases 1/2 phosphorylation, and cell contraction. These effects were blocked by Ki16425, an antagonist selective for LPA1 and LPA3 receptors, and by the LPA1-selective antagonist, AM095...
September 21, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28942351/sphingosine-1-phosphate-and-cancer
#12
REVIEW
Nigel J Pyne, Ashref El Buri, David R Adams, Susan Pyne
The bioactive lipid, sphingosine 1-phosphate (S1P) is produced by phosphorylation of sphingosine and this is catalysed by two sphingosine kinase isoforms (SK1 and SK2). Here we discuss structural functional aspects of SK1 (which is a dimeric quaternary enzyme) that relate to coordinated coupling of membrane association with phosphorylation of Ser225 in the 'so-called' R-loop, catalytic activity and protein-protein interactions (e.g. TRAF2, PP2A and Gq). S1P formed by SK1 at the plasma-membrane is released from cells via S1P transporters to act on S1P receptors to promote tumorigenesis...
September 15, 2017: Advances in Biological Regulation
https://www.readbyqxmd.com/read/28942144/microgravity-influences-maintenance-of-the-human-muscle-stem-progenitor-cell-pool
#13
Tohru Hosoyama, Shunji Ichida, Masatsugu Kanno, Reiichi Ishihara, Toshikatsu Hatashima, Koji Ueno, Kimikazu Hamano
Microgravity induces skeletal muscle atrophy; however, the underlying mechanism is not clarified. In particular, the influence of microgravity on human skeletal muscle stem/progenitor cells (SMPCs) is not well understood. In this study, we used induced pluripotent stem cell-derived human SMPCs to investigate the effect of microgravity on maintenance of the stem/progenitor cell pool. Human SMPCs were induced by free-floating spherical aggregation culture, and derivatized-SMPC spheres were maintained in a microgravity condition (10(-3) G) for 2 weeks using a clinostat rotation system...
September 20, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28942113/novel-serms-based-on-3-aryl-4-aryloxy-2h-chromen-2-one-skeleton-a-possible-way-to-dual-er%C3%AE-vegfr-2-ligands-for-treatment-of-breast-cancer
#14
Guoshun Luo, Xinyu Li, Guoqing Zhang, Chengzhe Wu, Zhengpu Tang, Linyi Liu, Qidong You, Hua Xiang
There is considerable interest in developing new SERMs as multifunctional agents in women's health. Development of dual selective estrogen receptor modulators/VEGFR-2 inhibitors (SERMs/V-2I) has been an attractive strategy for the discovery of new breast cancer therapeutic agents. Our previous efforts led to the preparation of a series of 3-aryl-4-anilino-2H-chromen-2-ones endowed with potent estrogen receptor binding affinity and anti-proliferative efficacy. In this study, various structurally related 3-aryl-4-anilino/aryloxy-2H-chromen-2-one analogues were rationally designed, synthesized and evaluated as a new chemo-type of dual ERα and VEGFR-2 inhibitors...
September 14, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28941043/cocr-enriched-medium-modulates-integrin-based-downstream-signaling-and-requires-a-set-of-inflammatory-genes-reprograming-in-vitro
#15
Célio J C Fernandes, Fabio Bezerra, Maiara das D do Carmo, Georgia Feltran, Mariana C Rossi, Rodrigo A da Silva, Pedro de M Padilha, Willian F Zambuzzi
Significant health concerns have been raised by the high levels of Cr and Co ions into whole blood as resulted of corrosion process released from biomedical implants, but very little is known about their biological behavior in governing cell metabolism. Thus, we prompted to address this issue by exploring the effects of CoCr enriched medium on both fibroblast and pre-osteoblast (pre-Ob) cells. Firstly, we showed there is a significant difference in Co and Cr releasing dependent on engineered surface, it being even more released in dual acid-etching treating surface (named w/DAE) than the machined surfaces (named wo/DAE)...
September 22, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28940173/liquiritigenin-ameliorates-memory-and-cognitive-impairment-through-cholinergic-and-bdnf-pathways-in-the-mouse-hippocampus
#16
Yong-Hyun Ko, Seung-Hwan Kwon, Seok-Yong Lee, Choon-Gon Jang
Liquiritigenin (LQ), a flavonoid extracted from the radix of Glycyrrhiza, has anti-inflammatory and neuroprotective properties. In this study, we evaluated the cognitive enhancing effects of LQ on learning and memory impairments induced by scopolamine (0.5 mg/kg, i.p.), a muscarinic antagonist, using the Y-maze, passive avoidance, and novel object recognition tests. A single administration of LQ significantly improved scopolamine-induced cognitive impairments in these behavioral tests. In addition, LQ dramatically inhibited acetylcholinesterase and thiobarbituric acid reactive substance activities in the hippocampus of scopolamine-induced mice in a dose-dependent manner...
September 22, 2017: Archives of Pharmacal Research
https://www.readbyqxmd.com/read/28939985/l1cam-drives-oncogenicity-in-esophageal-squamous-cell-carcinoma-by-stimulation-of-ezrin-transcription
#17
Jin-Cheng Guo, Yang-Min Xie, Li-Qiang Ran, Hui-Hui Cao, Chun Sun, Jian-Yi Wu, Zhi-Yong Wu, Lian-Di Liao, Wei-Jiang Zhao, Wang-Kai Fang, En-Min Li, Li-Yan Xu, Melitta Schachner, Jian-Jun Xie
L1 cell adhesion molecule (L1CAM) is highly expressed in various types of human cancers, displaying yet unknown molecular mechanisms underlying their oncogenic potential. Here, we found that L1CAM expression was significantly increased in esophageal squamous cell carcinoma (ESCC; n = 157) lesions compared with non-cancerous tissues. High tumorous L1CAM expression significantly correlated with reduced overall survival. Experimentally, L1CAM knockdown led to decreased cell growth, migration, and invasiveness in vitro, whereas overexpression of L1CAM showed the opposite effect...
September 22, 2017: Journal of Molecular Medicine: Official Organ of the "Gesellschaft Deutscher Naturforscher und Ärzte"
https://www.readbyqxmd.com/read/28939558/targeting-the-mapk-signaling-pathway-in-cancer-promising-preclinical-activity-with-the-novel-selective-erk1-2-inhibitor-bvd-523-ulixertinib
#18
Ursula A Germann, Brinley F Furey, William Markland, Russell R Hoover, Alex M Aronov, Jeffrey J Roix, Micheal Hale, Diane M Boucher, David A Sorrell, Gabriel Martinez-Botella, Matthew Fitzgibbon, Paul Shapiro, Michael J Wick, Ramin Samadani, Kathryn Meshaw, Anna Groover, Gary DeCrescenzo, Mark Namchuk, Caroline M Emery, Saurabh Saha, Dean J Welsch
Aberrant activation of signaling through the RAS-RAF-MEK-ERK (MAPK) pathway is implicated in numerous cancers, making it an attractive therapeutic target. Although BRAF- and MEK-targeted combination therapy has demonstrated significant benefit beyond single-agent options, the majority of patients develop resistance and disease progression after approximately 12 months. Reactivation of ERK signaling is a common driver of resistance in this setting. Here we report the discovery of BVD-523 (ulixertinib), a novel, reversible, ATP-competitive ERK1/2 inhibitor with high potency and ERK1/2 selectivity...
September 22, 2017: Molecular Cancer Therapeutics
https://www.readbyqxmd.com/read/28939254/inhibition-of-mek-erk1-2-map-kinase-signalling-pathway-reduces-rabies-virus-induced-pathologies-in-mouse-model
#19
Venkataravanappa Manjunatha, Karam Pal Singh, Mani Saminathan, Rajendra Singh, Nayakwadi Shivasharanappa, Channakeshava Sokke Umeshappa, Kuldeep Dhama, Gundallahalli Bayyappa Manjunathareddy
The extracellular signal-regulated kinase (ERK) pathway has been shown to regulate pathogenesis of many viral infections, but its role during rabies virus (RV) infection in vivo is not clear. In the present study, we investigated the potential role of MEK-ERK1/2 signalling pathway in the pathogenesis of rabies in mouse model and its regulatory effects on pro-inflammatory cytokines and other mediators of immunity, and kinetics of immune cells. Mice were infected with 25 LD50 of challenge virus standard (CVS) strain of RV by intracerebral (i...
September 19, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28939105/protein-kinase-c-eta-regulates-mcl-1-level-via-erk1
#20
Deepanwita Pal, Alakananda Basu
Protein kinase C (PKC)-eta (PKCη) is a member of the novel category of PKC family. It is overexpressed in breast cancer and was shown to inhibit apoptosis and contribute to chemoresistance. Since the anti-apoptotic Bcl-2 family protein myeloid cell leukemia-1 (Mcl-1) plays an important role in breast cancer cell survival and chemoresistance, we investigated if PKCη regulates Mcl-1 level. Silencing of PKCη decreased Mcl-1 in several breast cancer cells, including MCF-7 and T47D cells. PKCη depletion had no effect on MCL1 mRNA but the decrease in Mcl-1 by PKCη knockdown was blocked by proteasomal inhibitors, such as MG132 and lactacystin...
September 19, 2017: Cellular Signalling
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