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Quigley glaucoma

Elizabeth C Kimball, Mary E Pease, Matthew R Steinhart, Ericka N Oglesby, Ian Pitha, Cathy Nguyen, Harry A Quigley
We developed an explant model of the mouse eye and optic nerve that facilitates the study of retinal ganglion cell axons and mitochondria in the living optic nerve head (ONH) in an ex vivo environment. Two transgenic mouse strains were used, one expressing yellow fluorescent protein in selected axons and a second strain expressing cyan fluorescent protein in all mitochondria. We viewed an explanted mouse eye and optic nerve by laser scanning microscopy at and behind the ONH, the site of glaucoma injury. Explants from previously untreated mice were studied with the intraocular pressure (IOP) set artificially at normal or elevated levels for several hours...
April 14, 2017: Experimental Eye Research
Dan E Midgett, Mary E Pease, Joan L Jefferys, Mohak Patel, Christian Franck, Harry A Quigley, Thao D Nguyen
The objective of this study was to measure the pressure-induced deformation response of the human lamina cribrosa (LC) and analyze for variations with age and anatomical region. The posterior scleral cup of 8 eyes from 6 human donors was mounted onto a custom inflation chamber. A laser-scanning microscope was used for second harmonic generation (SHG) imaging of the collagen structure in the posterior volume of the LC at pressures from 5mmHg to 45mmHg. The SHG volumes were analyzed by the Fast-Fourier Iterative Digital Volume Correlation (DVC) algorithm for the three dimensional (3D) displacement field...
January 17, 2017: Acta Biomaterialia
Leonard A Levin, Megan E Crowe, Harry A Quigley
Within the field of glaucoma research, neuroprotection is defined as slowing the functional loss in glaucoma by a mechanism independent of lowering of intraocular pressure. There is currently a great potential for research surrounding neuroprotection as it relates to glaucoma. Anatomical targets for neuroprotection should focus on upstream rather than downstream factors, and could include any part of the retinal ganglion cell, the glia, especially astrocytes or Muller cells, and vasculature. The great number of anatomical targets is exceeded only by the number of possible biochemical pathways and potential treatments...
December 9, 2016: Experimental Eye Research
Yong Park, Dorette Ellis, Brett Mueller, Dorota Stankowska, Thomas Yorio
Recently, in a poll by Research America, a significant number of individuals placed losing their eyesight as having the greatest impact on their lives more so than other conditions, such as limb loss or memory loss. When they were also asked to rank which is the worst disease that could happen to them, blindness was ranked first by African-Americans and second by Caucasians, Hispanics, and Asians. Therefore, understanding the mechanisms of disease progression in the eye is extremely important if we want to make a difference in people's lives...
October 12, 2016: Handbook of Experimental Pharmacology
Nazlee Zebardast, Srinivasan Kavitha, Palaniswamy Krishnamurthy, David S Friedman, Monisha E Nongpiur, Tin Aung, Harry A Quigley, Pradeep Y Ramulu, Rengaraj Venkatesh
PURPOSE: To compare anterior segment optical coherence tomography (ASOCT) angle morphology before and after laser peripheral iridotomy (LPI) in a cohort of South Indian subjects with primary angle-closure suspect (PACS) or primary angle-closure/primary angle-closure glaucoma (PAC/PACG) and to examine baseline parameters associated with angle widening. DESIGN: Prospective observational study. PARTICIPANTS: A total of 244 subjects aged ≥30 years with PACS or PAC/PACG in at least 1 eye...
December 2016: Ophthalmology
Osamah J Saeedi, Luke Y Chang, Karun S Arora, Henry D Jampel, Harry A Quigley
We report a case of a suspected Sturge-Weber syndrome variant diagnosed at the age of 58 with the help of enhanced depth imaging spectral-domain ocular coherence tomography (EDI-SDOCT). A 58-year-old female with unilateral glaucoma was suspected to have choroidal vascular lesion, conjunctival angioma, and no facial port-wine stain who presented to the clinic with bleb dysesthesia many years after trabeculectomy. EDI-SDOCT was performed and revealed markedly increased choroidal thickness in the affected eye...
July 2016: Middle East African Journal of Ophthalmology
Jie Fu, Fengying Sun, Wenhua Liu, Yanfei Liu, Manasee Gedam, Qi Hu, Colleen Fridley, Harry A Quigley, Justin Hanes, Ian Pitha
Topical medications that inhibit the enzyme carbonic anhydrase (CAI) are widely used to lower intraocular pressure in glaucoma; however, their clinical efficacy is limited by the requirement for multiple-daily dosing, as well as side effects such as blurred vision and discomfort on drop instillation. We developed a biodegradable polymer microparticle formulation of the CAI dorzolamide that produces sustained lowering of intraocular pressure after subconjunctival injection. Dorzolamide was ion paired with sodium dodecyl sulfate (SDS) and sodium oleate (SO) with 0...
September 6, 2016: Molecular Pharmaceutics
Ericka N Oglesby, Gülgün Tezel, Elizabeth Cone-Kimball, Matthew R Steinhart, Joan Jefferys, Mary E Pease, Harry A Quigley
PURPOSE: To study the detailed cellular and molecular changes in the mouse sclera subjected to experimental glaucoma. METHODS: Three strains of mice underwent experimental bead-injection glaucoma and were euthanized at 3 days and 1, 3, and 6 weeks. Scleral protein expression was analyzed with liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) using (16)O/(18)O labeling for quantification in 1- and 6-week tissues. Sclera protein samples were also analyzed with immunoblotting with specific antibodies to selected proteins...
2016: Molecular Vision
Alison Flatau, Francisco Solano, Sana Idrees, Joan L Jefferys, Peter Volpe, Christopher Damion, Harry A Quigley
IMPORTANCE: Eyes of patients with glaucoma may be damaged during sleep. OBJECTIVE: To measure strains in glaucoma eyes and control eyes produced by mechanical force or deformation of the eye from contact when one side of the face rests against a pillow. DESIGN, SETTING, AND PARTICIPANTS: This study took place in a clinic-based setting among 22 patients with glaucoma and 11 age-matched controls. The research was conducted at Wilmer Eye Institute between February 4, 2014, and December 2, 2014...
April 2016: JAMA Ophthalmology
Francisco Solano-Moncada, Malgorzata Dymerska, Joan L Jefferys, Harry A Quigley
PURPOSE: To determine how often glaucoma specialists set a target intraocular pressure (IOP) and how they respond when the target is not achieved. METHODS: We reviewed 250 randomly selected charts of glaucoma patients seen by 5 glaucoma specialists to identify whether a target IOP was specifically set and to detail the plan of action when the target was exceeded. RESULTS: A target IOP was present for at least 1 eye in 66% of patients (165/250)...
April 2016: Journal of Glaucoma
Harry A Quigley, Ian F Pitha, Derek S Welsbie, Cathy Nguyen, Matthew R Steinhart, Thao D Nguyen, Mary Ellen Pease, Ericka N Oglesby, Cynthia A Berlinicke, Katherine L Mitchell, Jessica Kim, Joan J Jefferys, Elizabeth C Kimball
PURPOSE: To determine if oral losartan treatment decreases the retinal ganglion cell (RGC) death caused by experimental intraocular pressure (IOP) elevation in mice. METHODS: We produced IOP increase in CD1 mice and performed unilateral optic nerve crush. Mice received oral losartan, spironolactone, enalapril, or no drug to test effects of inhibiting angiotensin receptors. IOP was monitored by Tonolab, and blood pressure was monitored by tail cuff device. RGC loss was measured in masked axon counts and RGC bodies by β-tubulin labeling...
2015: PloS One
Baptiste Coudrillier, Jacek K Pijanka, Joan L Jefferys, Adhiraj Goel, Harry A Quigley, Craig Boote, Thao D Nguyen
OBJECTIVE: The biomechanical behavior of the sclera determines the level of mechanical insult from intraocular pressure to the axons and tissues of the optic nerve head, as is of interest in glaucoma. In this study, we measure the collagen fiber structure and the strain response, and estimate the material properties of glaucomatous and normal human donor scleras. METHODS: Twenty-two posterior scleras from normal and diagnosed glaucoma donors were obtained from an eyebank...
2015: PloS One
Nazlee Zebardast, Jason F Solus, Harry A Quigley, Divya Srikumaran, Pradeep Y Ramulu
BACKGROUND: Previous studies suggest there are large variations in adherence of ophthalmologists with the American Academy of Ophthalmology's Preferred Practice Patterns (PPPs). The purpose of this study was to compare rates of compliance with glaucoma care guidelines between resident and glaucoma faculty physicians at a single institution. METHODS: Charts of resident continuity clinic or glaucoma faculty patients with primary open angle glaucoma (POAG), ocular hypertension (OHTN), or suspicion of glaucoma were reviewed during the 2005-6 academic year...
2015: BMC Ophthalmology
Baptiste Coudrillier, Jacek Pijanka, Joan Jefferys, Thomas Sorensen, Harry A Quigley, Craig Boote, Thao D Nguyen
The effects of diabetes on the collagen structure and material properties of the sclera are unknown but may be important to elucidate whether diabetes is a risk factor for major ocular diseases such as glaucoma. This study provides a quantitative assessment of the changes in scleral stiffness and collagen fiber alignment associated with diabetes. Posterior scleral shells from five diabetic donors and seven non-diabetic donors were pressurized to 30 mm Hg. Three-dimensional surface displacements were calculated during inflation testing using digital image correlation (DIC)...
July 2015: Journal of Biomechanical Engineering
Jacek K Pijanka, Martin T Spang, Thomas Sorensen, Jun Liu, Thao D Nguyen, Harry A Quigley, Craig Boote
PURPOSE: The collagen structure of the human peripapillary sclera plays a significant role in determining optic nerve head (ONH) biomechanics, and is therefore of interest in the study of glaucoma. The aim of the current work was to map the anisotropic collagen structure of the normal human peripapillary sclera as a function of tissue depth. METHODS: Wide-angle x-ray scattering was used to quantify collagen fibril orientation at 0.5 mm intervals across six 150 μm-thick serial sections through the peripapillary sclera of eight normal European-derived human eyes...
2015: PloS One
Barbara J Murienne, Joan L Jefferys, Harry A Quigley, Thao D Nguyen
Pathological changes in scleral glycosaminoglycan (GAG) content and in scleral mechanical properties have been observed in eyes with glaucoma and myopia. The purpose of this study is to investigate the effect of GAG removal on the scleral mechanical properties to better understand the impact of GAG content variations in the pathophysiology of glaucoma and myopia. We measured how the removal of sulphated GAG (s-GAG) affected the hydration, thickness and mechanical properties of the posterior sclera in enucleated eyes of 6-9 month-old pigs...
January 2015: Acta Biomaterialia
Elizabeth C Kimball, Cathy Nguyen, Matthew R Steinhart, Thao D Nguyen, Mary E Pease, Ericka N Oglesby, Brian C Oveson, Harry A Quigley
The purpose of this study was to assess the effect of a scleral cross-linking agent on susceptibility to glaucoma damage in a mouse model.CD1 mice underwent 3 subconjunctival injections of 0.5 M glyceraldehyde (GA) in 1 week, then had elevated intraocular pressure (IOP) induced by bead injection. Degree of cross-linking was measured by enzyme-linked immunosorbent assay (ELISA), scleral permeability was measured by fluorescence recovery after photobleaching (FRAP), and the mechanical effects of GA exposure were measured by inflation testing...
November 2014: Experimental Eye Research
Jacek K Pijanka, Elizabeth C Kimball, Mary E Pease, Ahmed Abass, Thomas Sorensen, Thao D Nguyen, Harry A Quigley, Craig Boote
PURPOSE: The organization of scleral collagen helps to determine the eye's biomechanical response to intraocular pressure (IOP), and may therefore be important in glaucoma. This study provides a quantitative assessment of changes in scleral collagen fibril organization in bead-induced murine experimental glaucoma. METHODS: Wide-angle X-ray scattering was used to study the effect of bead-induced glaucoma on posterior scleral collagen organization in one eye of 12 CD1 mice, with untreated fellow eyes serving as controls...
October 2014: Investigative Ophthalmology & Visual Science
Harry A Quigley
No abstract text is available yet for this article.
February 2015: Annals of Behavioral Medicine: a Publication of the Society of Behavioral Medicine
Yih-Chung Tham, Xiang Li, Tien Y Wong, Harry A Quigley, Tin Aung, Ching-Yu Cheng
PURPOSE: Glaucoma is the leading cause of global irreversible blindness. Present estimates of global glaucoma prevalence are not up-to-date and focused mainly on European ancestry populations. We systematically examined the global prevalence of primary open-angle glaucoma (POAG) and primary angle-closure glaucoma (PACG), and projected the number of affected people in 2020 and 2040. DESIGN: Systematic review and meta-analysis. PARTICIPANTS: Data from 50 population-based studies (3770 POAG cases among 140,496 examined individuals and 786 PACG cases among 112 398 examined individuals)...
November 2014: Ophthalmology
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