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https://www.readbyqxmd.com/read/29785458/high-resolution-standardization-reduces-delay-due-to-workflow-disruptions-in-laparoscopic-cholecystectomy
#1
Marco von Strauss Und Torney, Sohelia Aghlmandi, Jasmin Zeindler, Debora Nowakowski, Christian A Nebiker, Christoph Kettelhack, Rachel Rosenthal, Raoul A Droeser, Savas D Soysal, Henry Hoffmann, Robert Mechera
BACKGROUND: Optimal resource utilization in high-cost environments like operating theatres is fundamental in today's cost constrained health care systems. Interruptions of the surgical workflow, i.e. microcomplications (MC), lead to prolonged procedure times and higher costs and can be indicative of surgical mistakes. Reducing MC can improve operating room efficiency and prevent intraoperative complications. We, therefore, aimed to evaluate the impact of a high-resolution standardized laparoscopic cholecystectomy protocol (HRSL) on operative time and intraoperative interruptions in a teaching hospital...
May 21, 2018: Surgical Endoscopy
https://www.readbyqxmd.com/read/29785005/nanopore-sequencing-technology-a-new-route-for-the-fast-detection-of-unauthorized-gmo
#2
Marie-Alice Fraiture, Assia Saltykova, Stefan Hoffman, Raf Winand, Dieter Deforce, Kevin Vanneste, Sigrid C J De Keersmaecker, Nancy H C Roosens
In order to strengthen the current genetically modified organism (GMO) detection system for unauthorized GMO, we have recently developed a new workflow based on DNA walking to amplify unknown sequences surrounding a known DNA region. This DNA walking is performed on transgenic elements, commonly found in GMO, that were earlier detected by real-time PCR (qPCR) screening. Previously, we have demonstrated the ability of this approach to detect unauthorized GMO via the identification of unique transgene flanking regions and the unnatural associations of elements from the transgenic cassette...
May 21, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29784986/a-workflow-for-the-integrative-transcriptomic-description-of-molecular-pathology-and-the-suggestion-of-normalizing-compounds-exemplified-by-parkinson-s-disease
#3
Mohamed Hamed, Yvonne Gladbach, Steffen Möller, Sarah Fischer, Mathias Ernst, Stephan Struckmann, Alexander Storch, Georg Fuellen
The volume of molecular observations on human diseases in public databases is continuously increasing at accelerating rates. A bottleneck is their computational integration into a coherent description, from which researchers may derive new well-founded hypotheses. Also, the need to integrate data from different technologies (genetics, coding and regulatory RNA, proteomics) emerged in order to identify biomarkers for early diagnosis and prognosis of complex diseases and therefore facilitating the development of novel treatment approaches...
May 21, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29783911/can-predictive-modeling-identify-head-and-neck-oncology-patients-at-risk-for-readmission
#4
Amy M Manning, Keith A Casper, Kay St Peter, Keith M Wilson, Jonathan R Mark, Ryan M Collar
Objective Unplanned readmission within 30 days is a contributor to health care costs in the United States. The use of predictive modeling during hospitalization to identify patients at risk for readmission offers a novel approach to quality improvement and cost reduction. Study Design Two-phase study including retrospective analysis of prospectively collected data followed by prospective longitudinal study. Setting Tertiary academic medical center. Subjects and Methods Prospectively collected data for patients undergoing surgical treatment for head and neck cancer from January 2013 to January 2015 were used to build predictive models for readmission within 30 days of discharge using logistic regression, classification and regression tree (CART) analysis, and random forests...
May 1, 2018: Otolaryngology—Head and Neck Surgery
https://www.readbyqxmd.com/read/29782891/review-article-a-translational-view-of-cells-secretome-analysis-from-untargeted-proteomic-to-potential-circulating-biomarkers
#5
REVIEW
Sandra I Anjo, Bruno Manadas
The identification of circulating biomarkers capable of being used as routine for diagnosis, prognosis, risk stratification and therapeutic monitoring is still the major aim at clinical proteomics. However, the direct search in blood samples or other clinical relevant biofluids is frequently associated with technical limitations that makes the biomarker discovery process extremely difficult when using these types of samples. In this sense, the use of different approaches, such as the analysis of cells' secretomes, can be an important and promising complementary method for the identification and pre-selection of potential circulating biomarkers...
May 18, 2018: Biochimie
https://www.readbyqxmd.com/read/29782620/genomic-big-data-hitting-the-storage-bottleneck
#6
Louis Papageorgiou, Picasi Eleni, Sofia Raftopoulou, Meropi Mantaiou, Vasileios Megalooikonomou, Dimitrios Vlachakis
During the last decades, there is a vast data explosion in bioinformatics. Big data centres are trying to face this data crisis, reaching high storage capacity levels. Although several scientific giants examine how to handle the enormous pile of information in their cupboards, the problem remains unsolved. On a daily basis, there is a massive quantity of permanent loss of extensive information due to infrastructure and storage space problems. The motivation for sequencing has fallen behind. Sometimes, the time that is spent to solve storage space problems is longer than the one dedicated to collect and analyse data...
2018: EMBnet.journal
https://www.readbyqxmd.com/read/29782615/feasibility-study-applying-a-parametric-model-as-the-design-generator-for-3d-printed-orthosis-for-fracture-immobilization
#7
Jianyou Li, Hiroya Tanaka
Background: Applying 3D printing technology for the fabrication of custom-made orthoses provides significant advantages, including increased ventilation and lighter weights. Currently, the design of such orthoses is most often performed in the CAD environment, but creating the orthosis model is a time-consuming process that requires significant CAD experience. This skill gap limits clinicians from applying this technology in fracture treatment. The purpose of this study is to develop a parametric model as the design generator for 3D-printed orthoses for an inexperienced CAD user and to evaluate its feasibility and ease of use via a training and design exercise...
2018: 3D printing in medicine
https://www.readbyqxmd.com/read/29782582/surfaces-from-the-visual-past-recovering-high-resolution-terrain-data-from-historic-aerial-imagery-for-multitemporal-landscape-analysis
#8
Christopher Sevara, Geert Verhoeven, Michael Doneus, Erich Draganits
Historic aerial images are invaluable sources of aid to archaeological research. Often collected with large-format photogrammetric quality cameras, these images are potential archives of multidimensional data that can be used to recover information about historic landscapes that have been lost to modern development. However, a lack of camera information for many historic images coupled with physical degradation of their media has often made it difficult to compute geometrically rigorous 3D content from such imagery...
2018: Journal of archaeological method and theory
https://www.readbyqxmd.com/read/29782418/compliant-workflow-design-tracking-procedures-performed
#9
Cathy Thomas Hess
No abstract text is available yet for this article.
June 2018: Advances in Skin & Wound Care
https://www.readbyqxmd.com/read/29782151/a-computational-workflow-for-the-automated-generation-of-models-of-genetic-designs
#10
Göksel Mısırlı, Tramy Nguyen, James Alastair McLaughlin, Prashant Vaidyanathan, Timothy Jones, Douglas Densmore, Chris J Myers, Anil Wipat
Computational models are essential to engineer predictable biological systems and to scale up this process for complex systems. Computational modelling often requires expert knowledge and data to build models. Clearly, manual creation of models is not scalable for large designs. Despite several automated model construction approaches, computational methodologies to bridge knowledge in design repositories and the process of creating computational models has still not been established. This paper describes a workflow for automatic generation of computational models of genetic circuits from data stored in design repositories using existing standards...
May 21, 2018: ACS Synthetic Biology
https://www.readbyqxmd.com/read/29781988/automated-slide-scanning-and-segmentation-in-fluorescently-labeled-tissues-using-a-widefield-high-content-analysis-system
#11
Candice C Poon, Vincent Ebacher, Katherine Liu, Voon Wee Yong, John James Patrick Kelly
Automated slide scanning and segmentation of fluorescently-labeled tissues is the most efficient way to analyze whole slides or large tissue sections. Unfortunately, many researchers spend large amounts of time and resources developing and optimizing workflows that are only relevant to their own experiments. In this article, we describe a protocol that can be used by those with access to a widefield high-content analysis system (WHCAS) to image any slide-mounted tissue, with options for customization within pre-built modules found in the associated software...
May 3, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29781496/a-universal-platform-for-selection-and-high-resolution-phenotypic-screening-of-bacterial-mutants-using-the-nanowell-slide
#12
H Antypas, M Veses-Garcia, E Weibull, H Andersson-Svahn, A Richter-Dahlfors
The Petri dish and microtiter plate are the golden standard for selection and screening of bacteria in microbiological research. To improve on the limited resolution and throughput of these methods, we developed a universal, user-friendly platform for selection and high-resolution phenotypic screening based on the nanowell slide. This miniaturized platform has an optimal ratio between throughput and assay complexity, holding 672 nanowells of 500 nl each. As monoclonality is essential in bacterial genetics, we used FACS to inoculate each nanowell with a single bacterium in 15 min...
May 21, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29780843/adjustable-versus-fixed-loop-devices-for-femoral-fixation-in-acl-reconstruction-an-in-vitro-full-construct-biomechanical-study-of-surgical-technique-based-tibial-fixation-and-graft-preparation
#13
Patrick A Smith, Marina Piepenbrink, Shelby K Smith, Samuel Bachmaier, Asheesh Bedi, Coen A Wijdicks
Background: Femoral suspensory fixation for anterior cruciate ligament (ACL) reconstruction has evolved from fixed- to adjustable-loop devices. However, there are still controversies regarding undesired lengthening of adjustable-loop devices. Hypothesis: Adjustable-loop fixation will achieve similar elongation to that of fixed-loop devices, and intraoperative preconditioning will reduce initial elongation for adjustable-loop constructs. Study Design: Controlled laboratory study...
April 2018: Orthopaedic Journal of Sports Medicine
https://www.readbyqxmd.com/read/29780386/automated-processing-and-evaluation-of-anti-nuclear-antibody-indirect-immunofluorescence-testing
#14
Vincent Ricchiuti, Joseph Adams, Donna J Hardy, Alexander Katayev, James K Fleming
Indirect immunofluorescence (IIF) is considered by the American College of Rheumatology (ACR) and the international consensus on ANA patterns (ICAP) the gold standard for the screening of anti-nuclear antibodies (ANA). As conventional IIF is labor intensive, time-consuming, subjective, and poorly standardized, there have been ongoing efforts to improve the standardization of reagents and to develop automated platforms for assay incubation, microscopy, and evaluation. In this study, the workflow and performance characteristics of a fully automated ANA IIF system (Sprinter XL, EUROPattern Suite, IFA 40: HEp-20-10 cells) were compared to a manual approach using visual microscopy with a filter device for single-well titration and to technologist reading...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29780384/discrimination-between-human-leukocyte-antigen-class-i-bound-and-co-purified-hiv-derived-peptides-in-immunopeptidomics-workflows
#15
Thomas Partridge, Annalisa Nicastri, Anna E Kliszczak, Louis-Marie Yindom, Benedikt M Kessler, Nicola Ternette, Persephone Borrow
Elucidation of novel peptides presented by human leukocyte antigen (HLA) class I alleles by immunopeptidomics constitutes a powerful approach that can inform the rational design of CD8+ T cell inducing vaccines to control infection with pathogens such as human immunodeficiency virus type 1 (HIV-1) or to combat tumors. Recent advances in the sensitivity of liquid chromatography tandem mass spectrometry instrumentation have facilitated the discovery of thousands of natural HLA-restricted peptides in a single measurement...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29779911/piloting-medication-histories-in-a-pediatric-postanesthesia-care-unit
#16
Nathan Lake, Humaira Nawer, Deborah Wagner
PURPOSE: Develop a medication history process for pediatric postanesthesia care unit (PACU) patients to identify discrepancies between home and inpatient medications and prevent medication errors. DESIGN: Pilot an evidence-based practice change to perform PACU medication histories. METHODS: Inpatients or surgical admissions to general care units at a pediatric tertiary care 348-bed hospital ages 2-18 years were included. Parents/guardians were asked about their child's prescription and over-the-counter medications, allergies, and adherence...
May 18, 2018: Journal of Perianesthesia Nursing: Official Journal of the American Society of PeriAnesthesia Nurses
https://www.readbyqxmd.com/read/29779722/the-impact-of-using-an-intravenous-workflow-management-system-ivwms-on-cost-and-patient-safety
#17
Alex C Lin, Yihong Deng, Hilal Thaibah, John Hingl, Jonathan Penm, Marianne F Ivey, Mark Thomas
PURPOSE: The aim of this study was to determine the financial costs associated with wasted and missing doses before and after the implementation of an intravenous workflow management system (IVWMS) and to quantify the number and the rate of detected intravenous (IV) preparation errors. METHOD: A retrospective analysis of the sample hospital information system database was conducted using three months of data before and after the implementation of an IVWMS System (DoseEdge® ) which uses barcode scanning and photographic technologies to track and verify each step of the preparation process...
July 2018: International Journal of Medical Informatics
https://www.readbyqxmd.com/read/29779657/collection-culturing-and-genome-analyses-of-tropical-marine-filamentous-benthic-cyanobacteria
#18
Nathan A Moss, Tiago Leao, Evgenia Glukhov, Lena Gerwick, William H Gerwick
Decreasing sequencing costs has sparked widespread investigation of the use of microbial genomics to accelerate the discovery and development of natural products for therapeutic uses. Tropical marine filamentous cyanobacteria have historically produced many structurally novel natural products, and therefore present an excellent opportunity for the systematic discovery of new metabolites via the information derived from genomics and molecular genetics. Adequate knowledge transfer and institutional know-how are important to maintain the capability for studying filamentous cyanobacteria due to their unusual microbial morphology and characteristics...
2018: Methods in Enzymology
https://www.readbyqxmd.com/read/29779398/handoff-tool-enabling-standardized-transitions-between-the-emergency-department-and-the-hospitalist-inpatient-service-at-a-major-cancer-center
#19
Carmen E Gonzalez, Norman Brito-Dellan, Srinivas R Banala, David Rubio, Mohamed Ait Aiss, Terry W Rice, Karen Chen, Diane C Bodurka, Carmelita P Escalante
Communication failures during patient handoff can lead to serious errors. A quality improvement team created a standardized handoff tool/process (DE-PASS: Decisive problem requiring admission, Evaluation time, Patient summary, Acute issues/action list, Situation unfinished/awareness, Signed out to) for admitting patients from the emergency department (ED) to the hospitalist inpatient service of a tertiary cancer center. DE-PASS mirrors the institution's ED workflow, stratifies patients as stable/urgent/emergent, and establishes requirements for verbal and email communications between providers...
May 1, 2018: American Journal of Medical Quality: the Official Journal of the American College of Medical Quality
https://www.readbyqxmd.com/read/29778526/rt-qpcr-analysis-of-human-melanoma-progression-related-genes-a-novel-workflow-for-selection-and-validation-of-candidate-reference-genes
#20
Marcelina E Janik, Sabina Szwed, Paweł Grzmil, Radosław Kaczmarek, Marcin Czerwiński, Dorota Hoja-Łukowicz
The objective of this study was to identify a normalizer or combination of normalizers for quantitative evaluation of the expression of a target gene of interest during melanoma progression. Adult melanocytes, uveal primary melanoma cells and cutaneous primary and metastatic melanoma cells were used to construct a panel of 14 experimental models reflecting cancer promotion and progression. Hypoxanthine phosphoribosyltransferase 1 (HPRT1), glucuronidase beta (GUSB), ribosomal protein S23 (RPS23), phosphoglycerate kinase 1 (PGK1) and small nuclear ribonucleoprotein polypeptide A (SRNPA) were chosen as candidate housekeeping genes...
May 17, 2018: International Journal of Biochemistry & Cell Biology
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