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Hanna Taipaleenmäki, Nicholas H Farina, Andre J van Wijnen, Janet L Stein, Eric Hesse, Gary S Stein, Jane B Lian
Wnt signaling is implicated in bone formation and activated in breast cancer cells promoting primary and metastatic tumor growth. A compelling question is whether osteogenic miRNAs that increase Wnt activity for bone formation are aberrantly expressed in breast tumor cells to support metastatic bone disease. Here we report that miR-218-5p is highly expressed in bone metastases from breast cancer patients, but is not detected in normal mammary epithelial cells. Furthermore, inhibition of miR-218-5p impaired the growth of bone metastatic MDA-MB-231 cells in the bone microenvironment in vivo...
October 12, 2016: Oncotarget
Pinling Lai, Qiancheng Song, Cheng Yang, Zhen Li, Sichi Liu, Bin Liu, Mangmang Li, Hongwen Deng, Daozhang Cai, Dadi Jin, Anling Liu, Xiaochun Bai
Osteoblast dysfunction is a major cause of age-related bone loss, but the mechanisms underlying changes in osteoblast function with aging are poorly understood. This study demonstrates that osteoblasts in aged mice exhibit markedly impaired adhesion to the bone formation surface and reduced mineralization in vivo and in vitro. Rictor, a specific component of the mechanistic target of rapamycin complex 2 (mTORC2) that controls cytoskeletal organization and cell survival, is downregulated with aging in osteoblasts...
October 13, 2016: Cell Death & Disease
Jianjun Gu, Rong Xu, Yaxing Li, Jianhe Zhang, Shousen Wang
To explore the effects of microRNA-218 (miR-218) on glioma cell lines and the related mechanism. U251 and U87 cells were transfected with negative control, miR-218 mimic or miR-218 inhibitor using lipofectamine 2000. The expressions of mRNA and proteins were detected with qRT-PCR and Western blotting. The cell proliferation, apoptosis, migration and invasion were studied using MTT, flow cytometry, Transwell assay and scratch-wound assay, respectively. The targeting effect of HMGB1 by miR-218 was measured with luciferase reporter assay...
2016: American Journal of Translational Research
Le-Le Wang, Lei Wang, Xiao-Ying Wang, Di Shang, Sheng-Jie Yin, Li-Li Sun, Hong-Bo Ji
The present study aims to investigate the effects of microRNA-218 (miR-218) on the proliferation, migration, invasion, and apoptosis of gastric cancer (GC) cells by targeting LIM and SH3 domain protein 1 (LASP1). The GC cells in the logarithmic phase were selected and divided into five groups: the blank group, negative control (NC) group, miR-218 inhibitors group, miR-218 inhibitors + siLASP1 group, and miR-218 mimics + siLASP1 group. The miR-218 expression in each group was also detected by qRT-PCR. The CCK8 assay, Transwell migration, and invasion assays and flow cytometry were performed to determine the effects of miR-218 on cell proliferation, migration, invasion, and apoptosis of GC cells...
September 30, 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
Yichun Kong, Bei Sun, Quanhong Han, Shuang Han, Yuchuan Wang, Ying Chen
Following the publication of this article, it was brought to our attention that five of the Figures contained in this study were published entirely, or in part, in the following publication, on which several of us were co-authors: Han S, Kong YC, Sun B, Han QH, Chen Y and Wang YC: microRNA-218 inhibits oxygen-induced retinal neovascularization via reducing the expression of roundabout 1. Chin Med J (Engl) 129: 709-715, 2016. While we had intended that these papers offered different research perspectives, we were reminded of the fact that, in submitting the article to International Journal of Molecular Medicine, the work described therein was required to be "original research that has not been published previously, and is not under consideration for publication elsewhere, in whole or in part"...
September 23, 2016: International Journal of Molecular Medicine
Guojun Wang, Yang Fu, Guanghui Liu, Yanwei Ye, Xiefu Zhang
microRNAs (miRNAs) play an important role in carcinogenesis. miR-218 is one of the most known miRNAs and has been demonstrated to inhibit progression in gastric cancer. However, the underlying molecular mechanism is not established. In this study, qRT-PCR and western blot indicated that miR-218 was down-regulated in gastric cancer cell lines SGC7901 and BGC823 compared to that in normal gastric epithelial cells GES-1. MTT and wound scratch assays suggested that overexpression of miR-218 markedly suppressed cell proliferation, migration, and EMT of gastric cancer cells...
September 16, 2016: Oncology Research
Tomohiro Kumamoto, Naohiko Seki, Hiroko Mataki, Keiko Mizuno, Kazuto Kamikawaji, Takuya Samukawa, Keiichi Koshizuka, Yusuke Goto, Hiromasa Inoue
The development of targeted molecular therapies has greatly benefited patients with lung adenocarcinomas. In contrast, these treatments have had little benefit in the management of lung squamous cell carcinoma (lung SCC). Therefore, new treatment options based on current genomic approaches are needed for lung SCC. Aberrant microRNA (miRNA) expression has been shown to promote lung cancer development and aggressiveness. Downregulation of microRNA-218 (miR-218) was frequently observed in our miRNA expression signatures of cancers, and previous studies have shown an antitumor function of miR-218 in several types of cancers...
September 13, 2016: International Journal of Oncology
Fujiao Duan, Kaijuan Wang, Liping Dai, Xia Zhao, Yajing Feng, Chunhua Song, Shuli Cui, Chengzeng Wang
BACKGROUND: Mounting evidence showed that microRNAs may be useful as prognostic biomarkers of cancer. Therefore, we summarize the predictive role of microRNA-218 (miR-218) for survival in patients with various cancers. METHODS: We performed a systematic literature review and assessed the quality of included studies based on Meta-analysis of Observational Studies in Epidemiology group (MOOSE). Hazard ratios (HRs) with corresponding 95% confidence intervals (CIs) were calculated to assess the correlation between miR-218 expression and prognosis of different cancers...
September 2016: Medicine (Baltimore)
Weiwei Luo, Xiao Liang, Songqian Huang, Xiaojuan Cao
Vascular endothelial growth factor A (VEGFA) is the most studied and the best characterized member of the VEGF family and is a key regulator of angiogenesis via its ability to affect the proliferation, migration, and differentiation of endothelial cells. In this study, the full-length cDNAs encoding VEGFAa and VEGFAb from pond loach, Misgurnus anguillicaudatus, were isolated. The VEGFAa is constituted by an open reading frame (ORF) of 570bp encoding for a peptide of 189 amino acid residues, a 639bp 5'-untranslated region (UTR) and a 2383bp 3' UTR...
August 8, 2016: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Katharina Ilm, Steffen Fuchs, Giridhar Mudduluru, Ulrike Stein
Metastasis is a multistep molecular network process, which is lethal for more than 90% of the cancer patients. Understanding the regulatory functions of metastasis-inducing molecules is in high demand for improved therapeutic cancer approaches. Thus, we studied the post-transcriptional regulation of the crucial carcinogenic and metastasis-mediating molecule metastasis associated in colon cancer 1 (MACC1). In silico analysis revealed MACC1 as a potential target of miR-218, a tumor suppressor miRNA. Expression of these two molecules inversely correlated in colorectal cancer (CRC) cell lines...
July 23, 2016: Oncotarget
Griet Conickx, Pieter Mestdagh, Francisco Avila Cobos, Fien M Verhamme, Tania Maes, Bart M Vanaudenaerde, Leen J M Seys, Lies Lahousse, Richard Y Kim, Alan C Hsu, Peter A Wark, Philip M Hansbro, Guy F Joos, Jo Vandesompele, Ken R Bracke, Guy G Brusselle
RATIONALE: Since aberrant expression of microRNAs (miRNAs) can have a detrimental role in disease pathogenesis, we aimed to identify dysregulated miRNAs in lung tissue of patients with COPD. METHODS: We performed miRNA and mRNA profiling - using high throughput stem-loop RT-qPCR and mRNA microarray, respectively - on lung tissue of 30 patients (screening cohort) encompassing 8 never smokers, 10 smokers without airflow limitation and 12 smokers with COPD. Differential expression of microRNA-218-5p (miR-218-5p) was validated by RT-qPCR in an independent cohort of 71 patients, an in vivo murine model of COPD, and primary human bronchial epithelial cells (HBECs)...
July 13, 2016: American Journal of Respiratory and Critical Care Medicine
X X Wang1, S J Ge2, X L Wang2, L X Jiang1, M F Sheng2, J J Ma2
The aim of the present study was to investigate the clinical significance of microRNA-218 (miR-218) in gastric cancer. We enrolled 112 patients having undergone surgery for gastric cancer between May 2008 and June 2014. Expression of miR-218 was determined by real-time quantitative reverse transcription-polymerase chain reaction. Survival curves were plotted using the Kaplan-Meier method and compared by the log-rank test. We found that miR-218 expression was significantly downregulated in gastric cancer tissues compared to adjacent normal tissues (P < 0...
2016: Genetics and Molecular Research: GMR
Jianming Wang, Yulin Ying, Shumei Bo, Guangjun Li, Fei Yuan
The present study assessed the expression profiles of numerous microRNAs (miRs; miR-141, miR-187, miR-206, miR-218, miR-335 and miR-204) in 25 pairs of renal cell carcinoma (RCC) tissue samples and adjacent non-cancerous tissue. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis revealed that miR‑218 was significantly downregulated in RCC tissue samples. Next, web‑based algorithms were used to identify B‑cell lymphoma (BCL)9 as a possible target of miR‑218, which was confirmed by a subsequent luciferase assay...
August 2016: Molecular Medicine Reports
Zhaojing Jiang, Qiancheng Song, Rong Zeng, Jing Li, Jingyu Li, Xiaochun Lin, Xing Chen, Jiren Zhang, Yanfang Zheng
Repeated infection with high-risk HPV is a major cause for the development and metastasis of human cervical cancer, even though the mechanism of the metastasis is still not completely understood. Here, we reported that miR-218 (microRNA-218) was downregulated in cervical cancer tissues, especially in metastatic cancer tissues. We found that miR-218 expression was associated with clinicopathological characteristics of patients with cervical cancer. MiR-218 overexpression inhibited Epithelial-Mesenchymal Transition (EMT), migration and invasiveness of cervical cancer cells in vitro...
June 6, 2016: Oncotarget
Mayuko Kato, Akira Kurozumi, Yusuke Goto, Ryosuke Matsushita, Atsushi Okato, Rika Nishikawa, Ichiro Fukumoto, Keiichi Koshizuka, Tomohiko Ichikawa, Naohiko Seki
Our recent studies of microRNA (miRNA) expression signatures of prostate cancer (PCa) showed that six miRNAs (specifically, miR-26a, miR-26b, miR-29a, miR-29b, miR-29c and miR-218) were markedly reduced in cancer tissues. Moreover, ectopic expression of these miRNAs suppressed PCa cell aggressiveness, indicating that these miRNAs acted in concert to regulate genes that promoted metastasis. Genome-wide gene expression analysis and in silico database analysis identified a total of 35 candidate genes that promoted metastasis and were targeted by these 6 miRNAs...
June 9, 2016: Journal of Human Genetics
Jing-Jing Liu, Cui-Mei Zhao, Zhi-Gang Li, Yu-Mei Wang, Wei Miao, Xiu-Juan Wu, Wen-Jing Wang, Chang Liu, Duo Wang, Kang Wang, Li Li, Lu-Ying Peng
MicroRNAs (miRNAs) have been identified as key players in cardiomyocyte hypertrophy, which is associated with significant risks of heart failure. However, many microRNAs are still not recognized for their functions in pathophysiological processes. In this study, we evaluated effects of miR-218 in cardiomyocyte hypertrophy using both in vitro and in vivo models. We found that miR-218 was evidently downregulated in a transverse aortic constriction (TAC) mouse model. Overexpression of miR-218 is sufficient to reduce hypertrophy, whereas the suppression of miR-218 aggravates hypertrophy in primary cardiomyocytes induced by isoprenaline (ISO)...
2016: International Journal of Molecular Sciences
Fei Tian, Rui Li, Zhenzhu Chen, Yanting Shen, Jiafeng Lu, Xueying Xie, Qinyu Ge
Lung cancer is the leading cause of cancer deaths. Non-small-cell lung cancer (NSCLC) is the major type of lung cancer. The aim of this study was to characterize the expression profiles of miRNAs in adenocarcinoma (AC), one major subtype of NSCLC. In this study, the miRNAs were detected in normal, adjacent, and tumor tissues by next-generation sequencing. Then the expression levels of differential miRNAs were quantified by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). In the results, 259, 401, and 389 miRNAs were detected in tumor, adjacent, and normal tissues of pooled AC samples, respectively...
2016: BioMed Research International
Lu Lu, Hui Xu, Fei Luo, Xinlu Liu, Xiaolin Lu, Qianlei Yang, Junchao Xue, Chao Chen, Le Shi, Qizhan Liu
Cigarette smoking is the strongest risk factor for the development of lung cancer, the leading cause of cancer-related deaths. However, the molecular mechanisms leading to lung cancer are largely unknown. A long-noncoding RNA (lncRNA), CCAT1, regarded as cancer-associated, has been investigated extensively. Moreover, the molecular mechanisms of lncRNAs in regulation of microRNAs (miRNAs) induced by cigarette smoke remain unclear. In the present investigation, cigarette smoke extract (CSE) caused an altered cell cycle and increased CCAT1 levels and decreased miR-218 levels in human bronchial epithelial (HBE) cells...
August 1, 2016: Toxicology and Applied Pharmacology
Qinbo Yang, Jingang Cui, Peiwei Wang, Xiaoye Du, Wenjian Wang, Teng Zhang, Yu Chen
Myocardial fibrosis is the endpoint pathology common to many cardiovascular disorders. We have previously shown that apocynin (APO), a naturally occurring NADPH oxidase inhibitor, significantly prevents the development of isoproterenol (ISO)-induced myocardial injury and fibrogenesis. The current study investigated the changes in microRNAs (miRNAs) and their potential implication in the cardioprotective effects of APO. Integrative analyses of whole-genome miRNA and gene expression profiles were first performed, revealing that altered expression of miRNAs likely contributed to dysregulated expression of genes associated with multiple interconnected fibrogenic signaling pathways...
August 5, 2016: European Journal of Pharmacology
Shouhua Zhang, Zhengdong Hong, Qiang Li, Jun Lei, Hui Huang, Queling Liu
OBJECTIVE: To investigate the effect of microRNA-218 on the viability, apoptosis and invasion of renal cell carcinoma cells under hypoxia by targeted regulation of expression of chemokine receptor 7 (CXCR7). METHODS: The expression of miR-218 in renal cell carcinoma cell lines under normal and hypoxia conditions, as well in normal renal tubular epithelial cells (HK2) was measured using RT-PCR. MiR-218 mimic and NC were transfected into renal cell carcinoma cell line ACHN using Lipofectamine™ 2000...
May 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
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