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https://www.readbyqxmd.com/read/28231509/a-single-injection-of-protein-loaded-coacervate-gel-significantly-improves-cardiac-function-post-infarction
#1
H K Awada, D W Long, Z Wang, M P Hwang, K Kim, Y Wang
After myocardial infarction (MI), the heart undergoes fibrotic pathological remodeling instead of repair and regeneration. With multiple pathologies developing after MI, treatment using several proteins is expected to address this range of pathologies more effectively than a single-agent therapy. A factorial design of experiments study guided us to combine three complementary factors in one injection: tissue inhibitor of metalloproteinases-3 (TIMP-3) was embedded in a fibrin gel for signaling in the initial phase of the treatment, while basic fibroblast growth factor (FGF-2) and stromal cell-derived factor 1-alpha (SDF-1α) were embedded in heparin-based coacervates for sustained release and distributed within the same fibrin gel to exert their effects over a longer period...
February 17, 2017: Biomaterials
https://www.readbyqxmd.com/read/28231508/regulating-dynamic-signaling-between-hematopoietic-stem-cells-and-niche-cells-via-a-hydrogel-matrix
#2
Bhushan P Mahadik, Narayanan A K Bharadwaj, Randy H Ewoldt, Brendan A C Harley
Hematopoietic stem cells (HSC) reside in unique bone marrow niches and are influenced by signals from surrounding cells, the extracellular matrix (ECM), ECM-bound or diffusible biomolecules. Here we describe the use of a three-dimensional hydrogel to alter the balance of HSC-generated autocrine feedback and paracrine signals generated by co-cultured niche-associated cells. We report shifts in HSC proliferation rate and fate specification in the presence of lineage positive (Lin(+)) niche cells. Hydrogels promoting autocrine feedback enhanced expansion of early hematopoietic progenitors while paracrine signals from Lin(+) cells increased myeloid differentiation...
February 14, 2017: Biomaterials
https://www.readbyqxmd.com/read/28231262/oleanolic-acid-induces-migration-in-mv1lu-and-mda-mb-231-epithelial-cells-involving-egf-receptor-and-map-kinases-activation
#3
Ángel Bernabé-García, David Armero-Barranco, Sergio Liarte, María Ruzafa-Martínez, Antonio Jesús Ramos-Morcillo, Francisco José Nicolás
During wound healing, skin function is restored by the action of several cell types that undergo differentiation, migration, proliferation and/or apoptosis. These dynamics are tightly regulated by the evolution of the extra cellular matrix (ECM) contents along the process. Pharmacologically active flavonoids have shown to exhibit useful physiological properties interesting in pathological states. Among them, oleanolic acid (OA), a pentacyclic triterpene, shows promising properties over wound healing, as increased cell migration in vitro and improved wound resolution in vivo...
2017: PloS One
https://www.readbyqxmd.com/read/28230777/cryptomphalus-aspersa-mollusc-egg-extract-promotes-regenerative-effects-in-human-dermal-papilla-stem-cells
#4
María Teresa Alameda, Esther Morel, Concepción Parrado, Salvador González, Ángeles Juarranz
The aim of this study was to test, by an in vitro approach, whether a natural extract derived from eggs of the mollusc Cryptomphalus aspersa (e-CAF) that seems to present regenerative properties, can enhance the mobilization of human hair dermal papilla cells (HHDPCs) and play a role on tissue repair and regeneration. We have tested HHDPCs proliferation by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium-bromide (MTT) assay; cell migration by using a wound healing assay, as well as the modulation of the expression of cytoskeletal (F-actin and vimentin) and cell adhesion to the extracellular matrix (ECM) (vinculin and P-FAK) proteins...
February 21, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28229263/exosomes-derived-from-human-amniotic-epithelial-cells-accelerate-wound-healing-and-inhibit-scar-formation
#5
Bin Zhao, Yijie Zhang, Shichao Han, Wei Zhang, Qin Zhou, Hao Guan, Jiaqi Liu, Jihong Shi, Linlin Su, Dahai Hu
Wound healing is a highly orchestrated physiological process consisting of a complex events, and scarless wound healing is highly desired for the development and application in clinical medicine. Recently, we have demonstrated that human amniotic epithelial cells (hAECs) promoted wound healing and inhibited scar formation through a paracrine mechanism. However, exosomes (Exo) are one of the most important paracrine factors. Whether exosomes derived from human amniotic epithelial cells (hAECs-Exo) have positive effects on scarless wound healing have not been reported yet...
February 22, 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28228548/contact-guidance-requires-spatial-control-of-leading-edge-protrusion
#6
G R Ramirez-San Juan, P W Oakes, M L Gardel
In vivo, geometric cues from the extra-cellular matrix (ECM) are critical for the regulation of cell shape, adhesion and migration. During contact guidance, the fibrillar architecture of the ECM promotes an elongated cell shape and migration along the fibrils. The subcellular mechanisms by which cells sense ECM geometry and translate it into changes in shape and migration direction, are not understood. Here, we pattern linear fibronectin features to mimic fibrillar ECM and elucidate the mechanisms of contact guidance...
February 22, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28227439/three-dimensional-graphene-scaffold-for-cardiac-tissue-engineering-and-in-situ-electrical-recording
#7
S K Ameri, P K Singh, R D'Angelo, W Stoppel, L Black, S R Sonkusale, S K Ameri, P K Singh, R D'Angelo, W Stoppel, L Black, S R Sonkusale, L Black, P K Singh, W Stoppel, S R Sonkusale, R D'Angelo, S K Ameri
In this paper, we present a three-dimensional graphene foam made of few layers of CVD grown graphene as a scaffold for growing cardiac cells and recording their electrical activity. Our results show that graphene foam not only provides an excellent extra-cellular matrix (ECM) for the culture of such electrogenic cells but also enables recording of its extracellular electrical activity in-situ. Recording is possible due to graphene's excellent conductivity. In this paper, we present our results on the fabrication of the graphene scaffold and initial studies on the culture of cardiac cell lines such as HL-1 and recording of their real-time electrical activity...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28226817/effective-connectivity-matrix-for-neural-ensembles
#8
Qi She, Winnie K Y So, Rosa H M Chan, Qi She, Winnie K Y So, Rosa H M Chan, Rosa H M Chan, Qi She, Winnie K Y So
In this paper, we present an efficient framework to study the directional interactions within the multiple-input multiple-output (MIMO) biological neural network from spiketrain data. We used an efficient generalized linear model (GLM) with Laguerre basis functions to model a MIMO neural system, and developed an Effective Connectivity Matrix (ECM) to visualize excitatory and inhibitory connections within the neural network. A new causality representation was developed based on system dynamics. Statistical test was applied to identify the significance of the measured causality...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28225063/targeted-inhibition-of-focal-adhesion-kinase-attenuates-cardiac-fibrosis-and-preserves-heart-function-in-adverse-cardiac-remodeling
#9
Jie Zhang, Guangpu Fan, Hui Zhao, Zhiwei Wang, Fei Li, Peide Zhang, Jing Zhang, Xu Wang, Wei Wang
Cardiac fibrosis in post-myocardial infarction (MI), seen in both infarcted and non-infarcted myocardium, is beneficial to the recovery of heart function. But progressively pathological fibrosis impairs ventricular function and leads to poor prognosis. FAK has recently received attention as a potential mediator of fibrosis, our previous study reported that pharmacological inhibition of FAK can attenuate cardiac fibrosis in post MI models. However, the long-term effects on cardiac function and adverse cardiac remodelling were not clearly investigated...
February 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28224898/non-covalently-crosslinked-chitosan-nanofibrous-mats-prepared-by-electrospinning-as-substrates-for-soft-tissue-regeneration
#10
Chiara Tonda-Turo, Francesca Ruini, Martina Ramella, Francesca Boccafoschi, Piergiorgio Gentile, Emilia Gioffredi, Giuseppe Falvo D'Urso Labate, Gianluca Ciardelli
Chitosan (CS) membranes obtained by electrospinning are potentially ideal substrates for soft tissue engineering as they combine the excellent biological properties of CS with the extracellular matrix (ECM)-like structure of nanofibrous mats. However, the high amount of acid solvents required to spun CS solutions interferes with the biocompatibility of CS fibres. To overcome this limitation, novel CS based solutions were investigated in this work. Low amount of acidic acid (0.5M) was used and dibasic sodium phosphate (DSP) was introduced as ionic crosslinker to improve nanofibres water stability and to neutralize the acidic pH of electrospun membranes after fibres soaking in biological fluids...
April 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28224047/identification-of-extracellular-matrix-components-and-biological-factors-in-micronized-dehydrated-human-amnion-chorion-membrane
#11
Jennifer Lei, Lauren B Priddy, Jeremy J Lim, Michelle Massee, Thomas J Koob
Objective: The use of bioactive extracellular matrix (ECM) grafts such as amniotic membranes is an attractive treatment option for enhancing wound repair. In this study, the concentrations, activity, and distribution of matrix components, growth factors, proteases, and inhibitors were evaluated in PURION(®) Processed, micronized, dehydrated human amnion/chorion membrane (dHACM; MiMedx Group, Inc.). Approach: ECM components in dHACM tissue were assessed by using immunohistochemical staining, and growth factors, cytokines, proteases, and inhibitors were quantified by using single and multiplex ELISAs...
February 1, 2017: Advances in Wound Care
https://www.readbyqxmd.com/read/28222740/dibutyryl-camp-attenuates-pulmonary-fibrosis-by-blocking-myofibroblast-differentiation-via-pka-creb-cbp-signaling-in-rats-with-silicosis
#12
Yan Liu, Hong Xu, Yucong Geng, Dingjie Xu, Lijuan Zhang, Yi Yang, Zhongqiu Wei, Bonan Zhang, Shifeng Li, Xuemin Gao, Ruimin Wang, Xianghong Zhang, Darrell Brann, Fang Yang
BACKGROUND: Myofibroblasts play a major role in the synthesis of extracellular matrix (ECM) and the stimulation of these cells is thought to play an important role in the development of silicosis. The present study was undertaken to investigate the anti-fibrotic effects of dibutyryl-cAMP (db-cAMP) on rats induced by silica. METHODS: A HOPE MED 8050 exposure control apparatus was used to create the silicosis model. Rats were randomly divided into 4 groups: 1)controls for 16 w; 2)silicosis for 16 w; 3)db-cAMP pre-treatment; 4) db-cAMP post-treatment...
February 21, 2017: Respiratory Research
https://www.readbyqxmd.com/read/28221776/the-horizon-of-materiobiology-a-perspective-on-material-guided-cell-behaviors-and-tissue-engineering
#13
Yulin Li, Yin Xiao, Changsheng Liu
Although the biological functions of cell and tissue can be regulated by biochemical factors (e.g., growth factors, hormones), the biophysical effects of materials on the regulation of biological activity are receiving more attention. In this Review, we systematically summarize the recent progress on how biomaterials with controllable properties (e.g., compositional/degradable dynamics, mechanical properties, 2D topography, and 3D geometry) can regulate cell behaviors (e.g., cell adhesion, spreading, proliferation, cell alignment, and the differentiation or self-maintenance of stem cells) and tissue/organ functions...
February 21, 2017: Chemical Reviews
https://www.readbyqxmd.com/read/28221164/positive-and-negative-cues-for-modulating-neurite-dynamics-and-receptor-expression
#14
Melissa Wrobel, Harini Gayathari Sundararaghavan
Many current peripheral nerve repair strategies focus on delivering positive, growth promoting cues (e.g. extracellular matrix, ECM) while eliminating negative, growth inhibiting cues (e.g. chondroitin sulfate proteoglycans, CSPGs) at the injury site. We hypothesized that recapitulating the positive and negative cues of the peripheral nerve injury microenvironment would improve regeneration. First, we tested the effects of a characteristic CSPG, Chondroitin Sulfate A (CSA) on neurite dynamics of dissociated chick embryo dorsal root ganglion (DRG) neurons using time lapse video microscopy...
February 21, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28220831/the-distribution-of-the-apparent-diffusion-coefficient-as-an-indicator-of-the-response-to-chemotherapeutics-in-ovarian-tumour-xenografts
#15
Monique C Tourell, Ali Shokoohmand, Marietta Landgraf, Nina P Holzapfel, Patrina S P Poh, Daniela Loessner, Konstantin I Momot
Diffusion-weighted magnetic resonance imaging (DW-MRI) was used to evaluate the effects of single-agent and combination treatment regimens in a spheroid-based animal model of ovarian cancer. Ovarian tumour xenografts grown in non-obese diabetic/severe-combined-immunodeficiency (NOD/SCID) mice were treated with carboplatin or paclitaxel, or combination carboplatin/paclitaxel chemotherapy regimens. After 4 weeks of treatment, tumours were extracted and underwent DW-MRI, mechanical testing, immunohistochemical and gene expression analyses...
February 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28220141/adhesive-peptide-sequences-regulate-valve-interstitial-cell-adhesion-phenotype-and-extracellular-matrix-deposition
#16
Yan Wu, K Jane Grande-Allen, Jennifer L West
Knowledge of how extracellular matrix (ECM) binding impacts valve interstitial cells (VICs) is critical not only to better understanding the etiology of valvular diseases but also to constructing living valve substitutes that can grow and remodel. Use of ECM-mimicking adhesive peptides with specific affinity to different receptors provides insights into adhesion-mediated cell signaling and downstream outcomes. Expression of adhesion receptors by VICs was assessed by flow cytometry and used to guide the choice of peptides studied...
December 2016: Cellular and Molecular Bioengineering
https://www.readbyqxmd.com/read/28219742/the-use-of-nanoscaffolds-and-dendrimers-in-tissue-engineering
#17
REVIEW
Bapi Gorain, Muktika Tekade, Prashant Kesharwani, Arun K Iyer, Kiran Kalia, Rakesh Kumar Tekade
To avoid tissue rejection during organ transplantation, research has focused on the use of tissue engineering to regenerate required tissues or organs for patients. The biomedical applications of hyperbranched, multivalent, structurally uniform, biocompatible dendrimers in tissue engineering include the mimicking of natural extracellular matrices (ECMs) in the 3D microenvironment. Dendrimers are unimolecular architects that can incorporate a variety of biological and/or chemical substances in a 3D architecture to actively support the scaffold microenvironment during cell growth...
February 17, 2017: Drug Discovery Today
https://www.readbyqxmd.com/read/28218403/unveiling-gene-trait-relationship-by-cross-platform-meta-analysis-on-chinese-hamster-ovary-cell-transcriptome
#18
Liang Zhao, Hsu-Yuan Fu, Ravali Raju, Nandita Vishwanathan, Wei-Shou Hu
In the past few years, transcriptome analysis has been increasingly employed to better understand the physiology of Chinese hamster ovary (CHO) cells at a global level. As more transcriptome data accumulated, meta-analysis on data sets collected from various sources can potentially provide better insights on common properties of those cells. Here, we performed meta-analysis on transcriptome data of different CHO cell lines obtained using NimbleGen or Affymetrix microarray platforms. Hierarchical clustering, non-negative matrix factorization (NMF) analysis, and principal component analysis (PCA) accordantly showed the samples were clustered into two groups: one consists of adherent cells in serum-containing medium, and the other suspension cells in serum-free medium...
February 20, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28216298/cell-instructive-starpeg-heparin-collagen-composite-matrices
#19
Marcus Binner, Laura J Bray, Jens Friedrichs, Uwe Freudenberg, Mikhail V Tsurkan, Carsten Werner
: Polymer hydrogels can be readily modulated with regard to their physical properties and functionalized to recapitulate molecular cues of the extracellular matrix (ECM). However, they remain structurally different from the hierarchical supramolecular assemblies of natural ECM. Accordingly, we herein report a set of hydrogel composite materials made from starPEG-peptide conjugates, maleimide-functionalized heparin and collagen type I that combine semisynthetic and ECM-derived components...
February 16, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28215881/effects-of-treatment-of-periparturient-dairy-cows-with-recombinant-bovine-somatotropin-on-health-and-productive-and-reproductive-parameters
#20
P R B Silva, H F Soares, W D Braz, G D Bombardelli, J A Clapper, D H Keisler, R C Chebel
The objectives of the current experiment were to evaluate the effects of treating periparturient dairy cows with recombinant bovine somatotropin (rbST) on incidence of postpartum diseases and performance. Holstein (HO) and Jersey (JS) cows from 2 herds were enrolled in the experiment at 253 ± 3 d of gestation and assigned to the control (n = 432) and rbST125 (n = 437) treatments. Cows in the rbST125 treatment received 125 mg of rbST, weekly, from -21 to 21 d relative to calving. Blood sampled weekly, from -21 to 21 d relative to calving, from a subsample of cows was used to determine the concentrations of growth hormone (GH, HO = 106) and insulin-like growth factor 1 (IGF-1, HO = 147 and JS = 49)...
February 16, 2017: Journal of Dairy Science
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