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https://www.readbyqxmd.com/read/27732873/systematic-analysis-of-known-and-candidate-lysine-demethylases-in-the-regulation-of-myoblast-differentiation
#1
Yoichi Munehira, Ze Yang, Or Gozani
Histone methylation dynamics plays a critical role in cellular programming during development. For example, specific lysine methyltransferases (KMTs) and lysine demethylases (KDMs) have been implicated in the differentiation of mesenchymal stem cells into various cell lineages. However, a systematic and functional analysis for an entire family of KMT or KDM enzymes has not been performed. Here, we test the function of all the known and candidate KDMs in myoblast and osteoblast differentiation using the C2C12 cell differentiation model system...
October 11, 2016: Journal of Molecular Biology
https://www.readbyqxmd.com/read/27688818/the-emerging-role-of-lysine-methyltransferase-setd8-in-human-diseases
#2
Ciro Milite, Alessandra Feoli, Monica Viviano, Donatella Rescigno, Agostino Cianciulli, Amodio Luca Balzano, Antonello Mai, Sabrina Castellano, Gianluca Sbardella
SETD8/SET8/Pr-SET7/KMT5A is the only known lysine methyltransferase (KMT) that monomethylates lysine 20 of histone H4 (H4K20) in vivo. Lysine residues of non-histone proteins including proliferating cell nuclear antigen (PCNA) and p53 are also monomethylated. As a consequence, the methyltransferase activity of the enzyme is implicated in many essential cellular processes including DNA replication, DNA damage response, transcription modulation, and cell cycle regulation. This review aims to provide an overview of the roles of SETD8 in physiological and pathological pathways and to discuss the progress made to date in inhibiting the activity of SETD8 by small molecules, with an emphasis on their discovery, selectivity over other methyltransferases and cellular activity...
2016: Clinical Epigenetics
https://www.readbyqxmd.com/read/27639391/association-of-nutritional-parameters-with-clinical-outcomes-in-patients-with-acute-myeloid-leukemia-undergoing-haematopoietic-stem-cell-transplantation
#3
Annic Baumgartner, Noemi Zueger, Annika Bargetzi, Michael Medinger, Jakob R Passweg, Zeno Stanga, Beat Mueller, Mario Bargetzi, Philipp Schuetz
INTRODUCTION: In acute myeloid leukemia (AML) patients undergoing allogeneic haematopoietic stem cell transplantation (HSCT), there is uncertainty about the extent of influence nutritional parameters have on clinical outcomes. In this study, we investigated the association between initial body mass index (BMI) and weight loss during HSCT on clinical outcomes in a well-characterised cohort of AML patients. METHODS: We analysed data of the Basel stem-cell transplantation registry ('KMT Kohorte') including all patients with AML undergoing first allogeneic HSCT from January 2003 to January 2014...
2016: Annals of Nutrition & Metabolism
https://www.readbyqxmd.com/read/27426629/the-methyltransferase-suv39h1-links-the-sumo-pathway-to-hp1%C3%AE-marking-at-pericentric-heterochromatin
#4
Christèle Maison, Delphine Bailly, Jean-Pierre Quivy, Geneviève Almouzni
The trimethylation of histone H3 on lysine 9 (H3K9me3) - a mark recognized by HP1 that depends on the Suv39h lysine methyltransferases (KMTs) - has provided a basis for the reader/writer model to explain HP1 accumulation at pericentric heterochromatin in mammals. Here, we identify the Suv39h1 paralog, as a unique enhancer of HP1α sumoylation both in vitro and in vivo. The region responsible for promoting HP1α sumoylation (aa1-167) is distinct from the KMT catalytic domain and mediates binding to Ubc9. Tethering the 1-167 domain of Suv39h1 to pericentric heterochromatin, but not mutants unable to bind Ubc9, accelerates the de novo targeting of HP1α to these domains...
2016: Nature Communications
https://www.readbyqxmd.com/read/27407169/protein-lysine-methylation-by-seven-%C3%AE-strand-methyltransferases
#5
REVIEW
Pål Ø Falnes, Magnus E Jakobsson, Erna Davydova, Angela Ho, Jędrzej Małecki
Methylation of biomolecules is a frequent biochemical reaction within the cell, and a plethora of highly specific methyltransferases (MTases) catalyse the transfer of a methyl group from S-adenosylmethionine (AdoMet) to various substrates. The posttranslational methylation of lysine residues, catalysed by numerous lysine (K)-specific protein MTases (KMTs), is a very common and important protein modification, which recently has been subject to intense studies, particularly in the case of histone proteins. The majority of KMTs belong to a class of MTases that share a defining 'SET domain', and these enzymes mostly target lysines in the flexible tails of histones...
July 15, 2016: Biochemical Journal
https://www.readbyqxmd.com/read/27146893/set7-mediated-gli3-methylation-plays-a-positive-role-in-the-activation-of-sonic-hedgehog-pathway-in-mammals
#6
Lin Fu, Hailong Wu, Steven Y Cheng, Daming Gao, Lei Zhang, Yun Zhao
Hedgehog signaling plays very important roles in development and cancers. Vertebrates have three transcriptional factors, Gli1, Gli2 and Gli3. Among them, Gli3 is a very special transcriptional factor which closely resembles Cubitus interruptus (Ci, in Drosophila) structurally and functionally as a 'double agent' for Shh target gene expression. Here we show that Gli3 full-length, but not the truncated form, can be methylated at K436 and K595. This methylation is specifically catalyzed by Set7, a lysine methyltransferase (KMT)...
2016: ELife
https://www.readbyqxmd.com/read/27060979/field-fertility-of-liquid-stored-and-cryopreserved-flow-cytometrically-sex-sorted-stallion-sperm
#7
Z Gibb, C G Grupen, W M C Maxwell, L H A Morris
REASON FOR PERFORMING STUDY: The fertility of sex-sorted, cryopreserved stallion sperm must be improved for the sex-sorting technology to be applied commercially. OBJECTIVES: Optimise the conditions used to liquid store stallion sperm prior to sex-sorting, and assess the fertility of sperm following sex-sorting and cryopreservation. STUDY DESIGN: Both in vitro experiment and randomised controlled trial in healthy, client-owned mares. METHODS: Stallion ejaculates (n = 9) were diluted in either a skimmed milk (KMT) or BSA (I-BSA) based media to 25 × 10(6) sperm/mL directly (+SP25) or washed to remove seminal plasma and diluted to 25 or 111 × 10(6) sperm/mL (-SP25 and -SP111)...
April 8, 2016: Equine Veterinary Journal
https://www.readbyqxmd.com/read/26929405/the-mettl20-homologue-from-agrobacterium-tumefaciens-is-a-dual-specificity-protein-lysine-methyltransferase-that-targets-ribosomal-protein-l7-l12-and-the-%C3%AE-subunit-of-electron-transfer-flavoprotein-etf%C3%AE
#8
Jędrzej Małecki, Helge-André Dahl, Anders Moen, Erna Davydova, Pål Ø Falnes
Human METTL20 is a mitochondrial, lysine-specific methyltransferase that methylates the β-subunit of electron transfer flavoprotein (ETFβ). Interestingly, putative METTL20 orthologues are found in a subset of α-proteobacteria, including Agrobacterium tumefaciens Using an activity-based approach, we identified in bacterial extracts two substrates of recombinant METTL20 from A. tumefaciens (AtMETTL20), namely ETFβ and the ribosomal protein RpL7/L12. We show that AtMETTL20, analogous to the human enzyme, methylates ETFβ on Lys-193 and Lys-196 both in vitro and in vivo ETF plays a key role in mediating electron transfer from various dehydrogenases, and we found that its electron transferring ability was diminished by AtMETTL20-mediated methylation of ETFβ...
April 29, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/26806951/traceless-synthesis-of-asymmetrically-modified-bivalent-nucleosomes
#9
Carolin C Lechner, Ninad D Agashe, Beat Fierz
Nucleosomes carry extensive post-translational modifications (PTMs), which results in complex modification patterns that are involved in epigenetic signaling. Although two copies of each histone coexist in a nucleosome, they may not carry the same PTMs and are often differently modified (asymmetric). In bivalent domains, a chromatin signature prevalent in embryonic stem cells (ESCs), namely H3 methylated at lysine 4 (H3K4me3), coexists with H3K27me3 in asymmetric nucleosomes. We report a general, modular, and traceless method for producing asymmetrically modified nucleosomes...
February 18, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/26718112/the-association-between-shallow-vestibular-depth-and-peri-implant-parameters-a-retrospective-6-years-longitudinal-study
#10
Michal Halperin-Sternfeld, Hadar Zigdon-Giladi, Eli E Machtei
AIM: The aim of this study was to retrospectively evaluate the association between shallow vestibular depth (VD) and peri-implant parameters. MATERIAL AND METHODS: Peri-implant parameters were evaluated in 61 periodontal patients under regular supportive periodontal therapy. Clinical parameters included gingival index (GI), plaque index (PI), bleeding on probing (BOP), peri-implant pocket depths (PPD), mucosal recession (MR), relative attachment level (RAL), width and thickness of keratinized mucosa (KMW, KMT) and VD...
March 2016: Journal of Clinical Periodontology
https://www.readbyqxmd.com/read/26647926/a-randomized-comparative-study-of-two-techniques-to-optimize-the-root-coverage-using-a-porcine-collagen-matrix
#11
Danilo Maeda Reino, Luciana Prado Maia, Patrícia Garani Fernandes, Sergio Luis Scombatti de Souza, Mario Taba Junior, Daniela Bazan Palioto, Marcio Fermandes de Moraes Grisi, Arthur Belém Novaes Jr
The aim of this randomized controlled clinical study was to compare the extended flap technique (EFT) with the coronally advanced flap technique (CAF) using a porcine collagen matrix (PCM) for root coverage. Twenty patients with two bilateral gingival recessions, Miller class I or II on non-molar teeth were treated with CAF+PCM (control group) or EFT+PCM (test group). Clinical measurements of probing pocket depth (PPD), clinical attachment level (CAL), recession height (RH), keratinized tissue height (KTH), keratinized mucosa thickness (KMT) were determined at baseline, 3 and 6 months post-surgery...
October 2015: Brazilian Dental Journal
https://www.readbyqxmd.com/read/26492085/novel-function-of-lysine-methyltransferase-g9a-in-the-regulation-of-sox2-protein-stability
#12
Jae-Young Lee, Se-Hwan Lee, Sun-Hee Heo, Kwang-Soo Kim, Changhoon Kim, Dae-Kwan Kim, Jeong-Jae Ko, Kyung-Soon Park
G9a is a lysine methyltransferase (KMTase) for histone H3 lysine 9 that plays critical roles in a number of biological processes. Emerging evidence suggests that aberrant expression of G9a contributes to tumor metastasis and maintenance of a malignant phenotype in cancer by inducing epigenetic silencing of tumor suppressor genes. Here, we show that G9a regulates Sox2 protein stability in breast cancer cells. When G9a lysine methyltransferase activity was chemically inhibited in the ER(+) breast cancer cell line MCF7, Sox2 protein levels were decreased...
2015: PloS One
https://www.readbyqxmd.com/read/26378239/mitotic-protein-cspp1-interacts-with-cenp-h-protein-to-coordinate-accurate-chromosome-oscillation-in-mitosis
#13
Lijuan Zhu, Zhikai Wang, Wenwen Wang, Chunli Wang, Shasha Hua, Zeqi Su, Larry Brako, Minerva Garcia-Barrio, Mingliang Ye, Xuan Wei, Hanfa Zou, Xia Ding, Lifang Liu, Xing Liu, Xuebiao Yao
Mitotic chromosome segregation is orchestrated by the dynamic interaction of spindle microtubules with the kinetochores. During chromosome alignment, kinetochore-bound microtubules undergo dynamic cycles between growth and shrinkage, leading to an oscillatory movement of chromosomes along the spindle axis. Although kinetochore protein CENP-H serves as a molecular control of kinetochore-microtubule dynamics, the mechanistic link between CENP-H and kinetochore microtubules (kMT) has remained less characterized...
November 6, 2015: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/26317544/kmt-set7-9-affects-genotoxic-stress-response-via-the-mdm2-axis
#14
Larissa Lezina, Vasilisa Aksenova, Olga Fedorova, Daria Malikova, Oleg Shuvalov, Alexey V Antonov, Dmitri Tentler, Alexander V Garabadgiu, Gerry Melino, Nikolai A Barlev
Genotoxic stress inflicted by anti-cancer drugs causes DNA breaks and genome instability. DNA double strand breaks induced by irradiation or pharmacological inhibition of Topoisomerase II activate ATM (ataxia-telangiectasia-mutated) kinase signalling pathway that in turn triggers cell cycle arrest and DNA repair. ATM-dependent gamma-phosphorylation of histone H2Ax and other histone modifications, including ubiquitnylation, promote exchange of histones and recruitment of DNA damage response (DDR) and repair proteins...
September 22, 2015: Oncotarget
https://www.readbyqxmd.com/read/26247364/calmodulin-methyltransferase-is-required-for-growth-muscle-strength-somatosensory-development-and-brain-function
#15
Sitvanit Haziza, Roberta Magnani, Dima Lan, Omer Keinan, Ann Saada, Eli Hershkovitz, Nurit Yanay, Yoram Cohen, Yoram Nevo, Robert L Houtz, Val C Sheffield, Hava Golan, Ruti Parvari
Calmodulin lysine methyl transferase (CaM KMT) is ubiquitously expressed and highly conserved from plants to vertebrates. CaM is frequently trimethylated at Lys-115, however, the role of CaM methylation in vertebrates has not been studied. CaM KMT was found to be homozygously deleted in the 2P21 deletion syndrome that includes 4 genes. These patients present with cystinuria, severe intellectual disabilities, hypotonia, mitochondrial disease and facial dysmorphism. Two siblings with deletion of three of the genes included in the 2P21 deletion syndrome presented with cystinuria, hypotonia, a mild/moderate mental retardation and a respiratory chain complex IV deficiency...
August 2015: PLoS Genetics
https://www.readbyqxmd.com/read/26230995/moset1-histone-h3k4-methyltransferase-in-magnaporthe-oryzae-regulates-global-gene-expression-during-infection-related-morphogenesis
#16
Kieu Thi Minh Pham, Yoshihiro Inoue, Ba Van Vu, Hanh Hieu Nguyen, Toru Nakayashiki, Ken-Ichi Ikeda, Hitoshi Nakayashiki
Here we report the genetic analyses of histone lysine methyltransferase (KMT) genes in the phytopathogenic fungus Magnaporthe oryzae. Eight putative M. oryzae KMT genes were targeted for gene disruption by homologous recombination. Phenotypic assays revealed that the eight KMTs were involved in various infection processes at varying degrees. Moset1 disruptants (Δmoset1) impaired in histone H3 lysine 4 methylation (H3K4me) showed the most severe defects in infection-related morphogenesis, including conidiation and appressorium formation...
July 2015: PLoS Genetics
https://www.readbyqxmd.com/read/26171447/comparative-study-of-two-surgical-techniques-for-root-coverage-of-large-recessions-in-heavy-smokers
#17
COMPARATIVE STUDY
Danilo M Reino, Luciana P Maia, Arthur B Novaes, Sérgio L S Souza
Reduced root coverage due to diminished periodontal vascularity can be expected in heavy smokers. The aim of this study was to evaluate the root coverage obtained for large gingival recessions in heavy smokers using two different surgical techniques. Twenty heavy smokers were selected. Each patient had large, bilateral Miller class I or II gingival recessions (Control Group (CG): 3.30 ± 1.29; Test Group (TG): 3.45 ± 0.80) on nonmolar teeth. Clinical measurements of probing pocket depth (PPD), clinical attachment level (CAL), recession height (RH), keratinized mucosa height (KMH), and keratinized mucosa thickness (KMT) were determined at baseline and after 12 months...
2015: International Journal of Esthetic Dentistry
https://www.readbyqxmd.com/read/26115316/saccharomyces-cerevisiae-eukaryotic-elongation-factor-1a-eef1a-is-methylated-at-lys-390-by-a-mettl21-like-methyltransferase
#18
Magnus E Jakobsson, Erna Davydova, Jędrzej Małecki, Anders Moen, Pål Ø Falnes
The human methyltransferases (MTases) METTL21A and VCP-KMT (METTL21D) were recently shown to methylate single lysine residues in Hsp70 proteins and in VCP, respectively. The yet uncharacterized MTase encoded by the YNL024C gene in Saccharomyces cerevisiae shows high sequence similarity to METTL21A and VCP-KMT, as well as to their uncharacterized paralogues METTL21B and METTL21C. Despite being most similar to METTL21A, the Ynl024c protein does not methylate yeast Hsp70 proteins, which were found to be unmethylated on the relevant lysine residue...
2015: PloS One
https://www.readbyqxmd.com/read/25922909/the-prerc-protein-orca-organizes-heterochromatin-by-assembling-histone-h3-lysine-9-methyltransferases-on-chromatin
#19
Sumanprava Giri, Vasudha Aggarwal, Julien Pontis, Zhen Shen, Arindam Chakraborty, Abid Khan, Craig Mizzen, Kannanganattu V Prasanth, Slimane Ait-Si-Ali, Taekjip Ha, Supriya G Prasanth
Heterochromatic domains are enriched with repressive histone marks, including histone H3 lysine 9 methylation, written by lysine methyltransferases (KMTs). The pre-replication complex protein, origin recognition complex-associated (ORCA/LRWD1), preferentially localizes to heterochromatic regions in post-replicated cells. Its role in heterochromatin organization remained elusive. ORCA recognizes methylated H3K9 marks and interacts with repressive KMTs, including G9a/GLP and Suv39H1 in a chromatin context-dependent manner...
April 29, 2015: ELife
https://www.readbyqxmd.com/read/25843229/a-role-for-kmt1c-in-monocyte-to-dendritic-cell-differentiation-epigenetic-regulation-of-monocyte-differentiation
#20
Rutger J Wierda, Marieke Goedhart, Marja C J A van Eggermond, Alice F Muggen, Xanne M Miggelbrink, Sacha B Geutskens, Erik van Zwet, Geert W Haasnoot, Peter J van den Elsen
Monocytes play a key role in immune system function. Chromatin remodeling is crucial for various differentiation and gene regulation processes and is rather well studied in T cells. However, for monocytes not much is known regarding how the epigenetic machinery influences the differentiation into various effector cell types. In the work presented here, we explore the epigenetic underpinnings of monocyte differentiation. By transcriptional profiling we show that transcription of lysine methyltransferases (KMTs) and in particular KMT1c is markedly up regulated after differentiation of monocytes into immature dendritic cells (iDCs)...
June 2015: Human Immunology
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