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Jichen Bao, Mingtao Huang, Dina Petranovic, Jens Nielsen
The yeast Saccharomyces cerevisiae is widely used as a cell factory to produce recombinant proteins. However, S. cerevisiae naturally secretes only a few proteins, such as invertase and the mating alpha factor, and its secretory capacity is limited. It has been reported that engineering protein anterograde trafficking from the endoplasmic reticulum to the Golgi apparatus by the moderate overexpression of SEC16 could increase recombinant protein secretion in S. cerevisiae. In this study, the retrograde trafficking in a strain with moderate overexpression of SEC16 was engineered by overexpression of ADP-ribosylation factor GTP activating proteins, Gcs1p and Glo3p, which are involved in the process of COPI-coated vesicle formation...
March 12, 2018: AMB Express
Uche Godfrey Okeke, Deniz Akdemir, Ismail Rabbi, Peter Kulakow, Jean-Luc Jannink
The HarvestPlus program for cassava ( Crantz) fortifies cassava with β-carotene by breeding for carotene-rich tubers (yellow cassava). However, a negative correlation between yellowness and dry matter (DM) content has been identified. We investigated the genetic control of DM in white and yellow cassava. We used regional heritability mapping (RHM) to associate DM with genomic segments in both subpopulations. Significant segments were subjected to candidate gene analysis and candidates were validated with prediction accuracies...
March 2018: Plant Genome
Krystyna Ciarkowska
Effect of tourism, especially skiing activities, and urbanization on chemical and biochemical properties of soils in touristy town-Zakopane-was investigated. The concentration of heavy metals, nutrients, soil organic matter (SOM), dehydrogenase (DHA), invertase (IA) and urease (Ure) activities in soils from the town centre and out of the town centre was compared with the respective values of adjacent soils in protected areas (TNP). In order to evaluate a degree of contamination and risks of degradation enrichment factor (EF), ecological risk index (RI), Nemerov Pollution Index (PINemerov ) as well as enzyme activity index (EAI) were calculated...
March 4, 2018: Environmental Science and Pollution Research International
Lingxian Ye, Guangying Zhao, Wenchao Dou
A sensitive Point-of-Care Testing (POCT) with Au-Pt bimetallic nanoparticles (Au@Pt) functionalized silica nanoparticle (SiO2 NPs) and Fe3 O4 magnetic nanoparticles (Fe3 O4 NPs) was designed for the quantitative detection of Escherichia coli O157:H7 (E. coli O157:H7). The poly-(4-styrenesulfonic acid-co-maleic acid) (PSSMA) as a negatively charged polyelectrolyte can be easily coated on surface of the amino group modified SiO2 NPs via electrostatic force. PSSMA is also a good stabilizer for water-soluble bimetallic nanostructures...
May 15, 2018: Talanta
Chengrong Nie, Xing Yang, Nabeel Khan Niazi, Xiaoya Xu, Yuhui Wen, Jörg Rinklebe, Yong Sik Ok, Song Xu, Hailong Wang
In the current study, we conducted a field experiment using the test plant, Brassica chinesis L. (pak choi), to investigate the effect of sugarcane bagasse-derived biochar on the bioavailability of cadmium (Cd), copper (Cu) and lead (Pb), and the health of soil microbiota in a contaminated soil. Biochar application significantly (P < 0.05) increased pak choi yield. Bioavailability of heavy metals to plant shoots and roots decreased with increasing biochar application rates (at 0, 1.5, 2.25 and 3.0 t ha-1 )...
February 22, 2018: Chemosphere
Qianmin Jia, Muhammad Kamran, Shahzad Ali, Lefeng Sun, Peng Zhang, Xiaolong Ren, Zhikuan Jia
Background: In the arid and semi-arid areas of northern China, overexploitation of fertilizers and extensive irrigation with brackish groundwater have led to soil degradation and large areas of farmland have been abandoned. In order to improve the soil quality of abandoned farmland and make reasonable use of brackish groundwater, we conducted field trials in 2013 and 2014. Methods: In our study, we used three fertilization modes (CF, chemical fertilizer; OM, organic manure and chemical fertilizer; NF, no fertilizer) and three deficit irrigation levels (I0 : 0 mm; I75 : 75 mm; I150 : 150 mm)...
2018: PeerJ
Haoran Huang, Guangying Zhao, Wenchao Dou
Here we innovate a portable and quantitative immunochromatographic assay (ICA) with a personal glucose meter (PGM) as readout for the detection of Escherichia coli O157:H7 (E. coli O157:H7). The carboxyl group coated Fe3 O4 nanoparticles (MNPs) were synthesized by a one pot method and used as carriers of invertase and monoclonal antibody against E. coli O157:H7. Initially, the invertase and antibody double functionalized MNPs (Invertase-MNPs-IgG) conjugates were prepared and used as label probe in this assay system...
February 15, 2018: Biosensors & Bioelectronics
Vanda R Reis, Ana Paula G Bassi, Bianca C Cerri, Amanda R Almeida, Isis G B Carvalho, Reinaldo G Bastos, Sandra R Ceccato-Antonini
Even though contamination by bacteria and wild yeasts are frequently observed during fuel ethanol fermentation, our knowledge regarding the effects of both contaminants together is very limited, especially considering that the must composition can vary from exclusively sugarcane juice to a mixture of molasses and juice, affecting the microbial development. Here we studied the effects of the feedstock (sugarcane juice and molasses) and the co-culture of Lactobacillus fermentum and a wild Saccharomyces cerevisiae strain (rough colony and pseudohyphae) in single and multiple-batch fermentation trials with an industrial strain of S...
February 16, 2018: AMB Express
Jinheng Zhu, Jiyan Qi, Yongjun Fang, Xiaohu Xiao, Jiuhui Li, Jixian Lan, Chaorong Tang
Sucrose-metabolizing enzymes in plant leaves have hitherto been investigated mainly in temperate plants, and rarely conducted in tandem with gene expression and sugar analysis. Here, we investigated the sugar content, gene expression, and the activity of sucrose-metabolizing enzymes in the leaves of Hevea brasiliensis , a tropical tree widely cultivated for natural rubber. Sucrose, fructose and glucose were the major sugars detected in Hevea leaves at four developmental stages (I to IV), with starch and quebrachitol as minor saccharides...
2018: Frontiers in Plant Science
Bongeka Zuma, Mason B Dana, Dongfang Wang
Proper seed development requires coordinated growth among the three genetically distinct components, the embryo, the endosperm, and the seed coat. In Arabidopsis, embryo growth rate accelerates after endosperm cellularization, which requires a chromatin-remodeling complex, the FIS2-Polycomb Repressive Complex 2 (PRC2). After cellularization, the endosperm ceases to grow and is eventually absorbed by the embryo. This sequential growth pattern displayed by the endosperm and the embryo suggests a possibility that the supply of sugar might be shifted from the endosperm to the embryo upon endosperm cellularization...
2018: Frontiers in Plant Science
Frederico Mendonça Bahia, Gabriela Carneiro de Almeida, Lorena Pereira de Andrade, Christiane Gonçalves Campos, Lúcio Rezende Queiroz, Rayane Luzia Vieira da Silva, Patrícia Verardi Abdelnur, José Raimundo Corrêa, Maurizio Bettiga, Nádia Skorupa Parachin
Biosurfactants are biological tensioactive agents that can be used in the cosmetic and food industries. Rhamnolipids are glycolipid biosurfactants naturally produced by Pseudomonas aeruginosa and are composed of one or two rhamnose molecules linked to beta-hydroxy fatty acid chains. These compounds are green alternatives to petrochemical surfactants, but their large-scale production is still in its infancy, hindered due to pathogenicity of natural producer, high substrate and purification costs and low yields and productivities...
February 13, 2018: Scientific Reports
Renjie Li, Zhiqiang Hou, Hui Zou, Yongtao Wang, Xiaojun Liao
The activity, structure and morphology of mango soluble acid invertase (SAI) were investigated after high pressure processing (HPP) combined with mild temperature at 50-600MPa and 40-50°C. The activity of mango SAI was efficiently reduced by HPP at 50MPa/45 and 50°C, or 600MPa/40, 45 and 50°C, while it was increased by 10-30% after HPP at 50-200MPa/40°C. Significant antagonistic effect of pressure and temperature on the activity of SAI was observed at 50-400MPa/50°C. The secondary structure of SAI was not influenced by HPP...
March 2018: Food Research International
Lynette Lincoln, Sunil S More
This study focused on the purification and characterization of an extracellular β-d-fructofuranosidase or invertase from Aspergillus sojae JU12. The protein was purified by size exclusion chromatography with 5.41 fold and 10.87% recovery. The apparent molecular mass of the enzyme was estimated to be ~ 35 kDa using SDS-PAGE and confirmed by deconvoluted mass spectrometry. The fungal β-d-fructofuranosidase was suggested to be a monomer by native PAGE and zymography, and was found to be a glycoprotein possessing 68...
February 2018: 3 Biotech
M Govarthanan, R Mythili, T Selvankumar, S Kamala-Kannan, H Kim
In the present study, Helianthus annuus grown in arsenic- (As) and lead- (Pb) contaminated soil were treated with plant-growth promoting fungi Trichoderma sp. MG isolated from decayed wood and assessed for their phytoremediation efficiency. The isolate MG exhibited a high tolerance to As (650mg/L) and Pb (500mg/L), and could remove > 70% of metals in aqueous solution with an initial concentration of 100mg/L each. In addition, the isolate MG was screened for plant-growth-promoting factors such as siderophores, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, indole acetic acid (IAA) synthesis, and phosphate solubilisation...
January 23, 2018: Ecotoxicology and Environmental Safety
Xiaotong Guo, Xiaoguang Duan, Yongzhen Wu, Jieshan Cheng, Juan Zhang, Hongxia Zhang, Bei Li
Cell wall invertase plays important roles in the grain filling of crop plants. However, its functions in the improvement of grain nutrients are not investigated. In this work, the stable expression of cell wall invertase encoding genes from different plant species, and the contents of total starch, protein, amino acid, nitrogen, lipid and phosphorus were examined in transgenic maize plants. High expression of cell wall invertase gene conferred enhanced invertase activity and sugar content on transgenic plants, leading to increased grain yield and improved grain nutrients...
February 2, 2018: Journal of Agricultural and Food Chemistry
T A Woyengo, R Patterson, C L Levesque
Cold-pressed camelina cake (CPCC) is a fibrous co-product of camelina seed pressing and available for livestock feeding. However, information is lacking on the effect of supplementing fiber-degrading enzymes to CPCC-based diets on nutrient utilization by pigs. Experiment 1 determined the effect of multi-enzyme supplementation on standardized ileal digestibility (SID) of AA and NE value of CPCC for pigs. Six ileal-cannulated barrows (average initial BW = 36 kg) were fed 5 diets in 5 × 5 Latin square design with 1 added column to give 6 replicates per diet...
January 27, 2018: Journal of Animal Science
Si Shen, Li Zhang, Xiao-Gui Liang, Xue Zhao, Shan Lin, Ling-Hua Qu, Yun-Peng Liu, Zhen Gao, Yong-Ling Ruan, Shun-Li Zhou
Selective seed abortion is a survival strategy adopted by many species that sacrifices some seeds to allow the remaining ones to set. While evolutionally a successful approach, it causes huge losses to crop yield. A pollination time gap (PTG) was suggested to be related to the position-related grain abortion. To test this hypothesis, we developed a novel approach to alter the natural pattern of maize (Zea mays L.) pollination and to examine the impact of PTGs on kernel growth and the underlying physiological basis...
January 20, 2018: Journal of Experimental Botany
Shamitha Rao Morey, Tatsuro Hirose, Yoichi Hashida, Akio Miyao, Hirohiko Hirochika, Ryu Ohsugi, Junko Yamagishi, Naohiro Aoki
BACKGROUND: Rice is a major crop feeding the majority of the global population, and increasing its sink strength is one of the modes to alleviate the declining availability of food for the rapidly growing world population. We demonstrate a role for an important rice vacuolar invertase isoform, OsINV3, in sink strength determination. RESULTS: OsINV3 mutants showed shorter panicles with lighter and smaller grains, owing to a smaller cell size on the outer and inner surfaces of the palea and lemma as observed by scanning electron microscopy...
January 17, 2018: Rice
Yafei Shen, Ruimei Cheng, Wenfa Xiao, Shao Yang, Yan Guo, Na Wang, Lixiong Zeng, Lei Lei, Xiaorong Wang
The effects of forest management on carbon (C) sequestration are poorly understood, particularly in the Three Gorges Reservoir area. We aimed to identify the effects of forest management on C sequestration in Pinus massoniana plantations. An intact control forest (CK), a site undergoing regular shrub cutting with the simultaneous removal of residues (SC), a site under low-intensity thinning (LIT), and a site under high-intensity thinning (HIT) were compared for soil labile organic carbon (LOC), related enzyme activities, and soil characteristics...
January 12, 2018: Scientific Reports
S A Sheshadri, M J Nishanth, N Harita, P Brindha, S Bindu
Plant secondary metabolites are widely used in human disease treatment; though primary metabolism provides precursors for secondary metabolism, not much has been studied to unravel the link connecting both the processes. Most common form of gene regulation interconnecting diverse metabolism occurs at the transcriptional and/or posttranscriptional level mediated by regulatory cis-elements. The present study aims at understanding the common cis-elements network connecting the major primary metabolic enzyme, cell wall invertase (CWIN) and secondary metabolism genes in Nicotiana benthamiana (N...
November 13, 2017: Computational Biology and Chemistry
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