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https://www.readbyqxmd.com/read/28731585/bacterial-invertases-occurrence-production-biochemical-characterization-and-significance-of-transfructosylation
#1
REVIEW
Lynette Lincoln, Sunil S More
Invertase or β-D-fructofuranoside fructohydrolase (EC 3.2.1.26) was one of the foremost enzyme biocatalysts and established the primary concepts of most enzyme-kinetic principles. Invertases are glycoside hydrolases and occur mostly in microorganisms. Among microbial strains, for many decades yeast species have been extensively researched for invertase production, characterization, and applications in industries. Besides, limited literature is available on invertases from bacterial strains. The enzymic and molecular biological reports from bacterial invertases are scarce...
July 21, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28717209/non-target-effects-on-soil-microbial-parameters-of-the-synthetic-pesticide-carbendazim-with-the-biopesticides-cantharidin-and-norcantharidin
#2
Hainan Shao, Yalin Zhang
Considering the fact that biopesticides are increasingly used to replace synthetic pesticides in pest control, it is necessary to assess their ecotoxicity and especially their non-target effects on soil microorganisms, which is largely unknown. In this study, the effects of the synthetic pesticide carbendazim and the biopesticides (cantharidin and norcantharidin) on soil microbial parameters in a silt loam soil were evaluated. By using commercial formulations at the recommended and higher rates, both cantharidin and norcantharidin induced adverse effects on soil invertase, phosphatase activities and fungal gene structure, but these changes were transient...
July 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28694802/the-response-regulator-slr1588-regulates-spsa-but-is-not-crucial-for-salt-acclimation-of-synechocystis-sp-pcc-6803
#3
Kuo Song, Martin Hagemann, Xiaoming Tan, Xuefeng Lu
Cyanobacterial sucrose biosynthesis is stimulated under salt stress, which could be used for biotechnological sugar production. It has been shown that the response regulator Slr1588 negatively regulates the spsA gene encoding sucrose-phosphate synthase and mutation of the slr1588 gene also affected the salt tolerance of Synechocystis (Chen et al., 2014). The latter finding is contrary to earlier observations (Hagemann et al., 1997b). Here, we observed that ectopic expression of slr1588 did not restore the salt tolerance of the slr1588 mutant, making the essential function of this response regulator for salt tolerance questionable...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28690616/comparative-transcriptome-analysis-reveals-critical-function-of-sucrose-metabolism-related-enzymes-in-starch-accumulation-in-the-storage-root-of-sweet-potato
#4
Kai Zhang, Zhengdan Wu, Daobin Tang, Kai Luo, Huixiang Lu, Yingying Liu, Jie Dong, Xin Wang, Changwen Lv, Jichun Wang, Kun Lu
The starch properties of the storage root (SR) affect the quality of sweet potato (Ipomoea batatas (L.) Lam.). Although numerous studies have analyzed the accumulation and properties of starch in sweet potato SRs, the transcriptomic variation associated with starch properties in SR has not been quantified. In this study, we measured the starch and sugar contents and analyzed the transcriptome profiles of SRs harvested from sweet potatoes with high, medium, and extremely low starch contents, at five developmental stages [65, 80, 95, 110, and 125 days after transplanting (DAP)]...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28671003/effective-antigen-delivery-via-dual-entrapment-in-erythrocytes-and-autologous-plasma-beads
#5
Munazza Tamkeen Fatima, Ejaj Ahmad, Mehboob Hoque
Fibrin-based polymeric systems have now emerged as efficient carriers of drugs, growth factors, genes, and cells. Earlier, we have reported fibrin based plasma beads (PB), prepared from clotted whole plasma, as an efficient system for the controlled release of entrapped therapeutics. In the present study, we investigate the dual entrapment in erythrocytes and PB, as potential particulate antigen delivery system in rabbit and mice, with yeast invertase as the model antigen. Preparations used for immunization include- invertase entrapped in erythrocytes, the same further entrapped in PB, and crosslinked with glutaraldehyde...
July 3, 2017: Journal of Drug Targeting
https://www.readbyqxmd.com/read/28658609/postharvest-changes-in-lin5-down-regulated-plants-suggest-a-role-for-sugar-deficiency-in-cuticle-metabolism-during-ripening
#6
José G Vallarino, Trevor H Yeats, Eugenia Maximova, Jocelyn K Rose, Alisdair R Fernie, Sonia Osorio
The cell wall invertase gene (LIN5) was reported to be a key enzyme influencing sugar uptake of tomato (Solanum lycopersicum) fruit. It was additionally revealed to be a key regulator of total soluble solids content in fruit as well as for reproductive development, being mainly involved in flower development, early fruit and seed development but also in ripening. Here, we demonstrate that silencing of the LIN5 gene promotes changes affecting fruit cuticle development which has a direct effect on postharvest properties...
June 25, 2017: Phytochemistry
https://www.readbyqxmd.com/read/28651190/temporal-dynamics-of-the-compositions-and-activities-of-soil-microbial-communities-post-application-of-the-insecticide-chlorantraniliprole-in-paddy-soils
#7
Meng Wu, Jia Liu, Weitao Li, Ming Liu, Chunyu Jiang, Zhongpei Li
Chlorantraniliprole (CAP) is a newly developed insecticide widely used in rice fields in China. There has been few studies evaluating the toxicological effects of CAP on soil-associated microbes. An 85-day microcosm experiment was performed to reveal the dissipation dynamics of CAP in three types of paddy soils in subtropical China. The effects of CAP on microbial activities (microbial biomass carbon-MBC, basal soil respiration-BSR, microbial metabolic quotient-qCO2, acid phosphatase and sucrose invertase activities) in the soils were periodically evaluated...
June 23, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28645264/molecular-cloning-structure-phylogeny-and-expression-analysis-of-the-invertase-gene-family-in-sugarcane
#8
Liming Wang, Yuexia Zheng, Shihui Ding, Qing Zhang, Youqiang Chen, Jisen Zhang
BACKGROUND: Invertases (INVs) are key enzymes regulating sucrose metabolism and are here revealed to be involved in responses to environmental stress in plants. To date, individual members of the invertase gene family and their expression patterns are unknown in sugarcane due to its complex genome despite their significance in sucrose metabolism. RESULTS: In this study, based on comparative genomics, eleven cDNA and twelve DNA sequences belonging to 14 non-redundant members of the invertase gene family were successfully cloned from sugarcane...
June 23, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28637447/differential-gene-expression-at-different-stages-of-mesocarp-development-in-high-and-low-yielding-oil-palm
#9
Yick Ching Wong, Huey Fang Teh, Katharina Mebus, Tony Eng Keong Ooi, Qi Bin Kwong, Ka Loo Koo, Chuang Kee Ong, Sean Mayes, Fook Tim Chew, David R Appleton, Harikrishna Kulaveerasingam
BACKGROUND: The oil yield trait of oil palm is expected to involve multiple genes, environmental influences and interactions. Many of the underlying mechanisms that contribute to oil yield are still poorly understood. In this study, we used a microarray approach to study the gene expression profiles of mesocarp tissue at different developmental stages, comparing genetically related high- and low- oil yielding palms to identify genes that contributed to the higher oil-yielding palm and might contribute to the wider genetic improvement of oil palm breeding populations...
June 21, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28634826/tai-vacuolar-invertase-orthologs-the-interspecific-variability-in-tomato-plants-solanum-section-lycopersicon
#10
M A Slugina, A V Shchennikova, E Z Kochieva
Understanding the genetic mechanisms underlying carbohydrate metabolism can promote the development of biotechnological advances in fruit plants. The flesh tomato fruit represents an ideal system for examining the role of sucrose cleavage enzymes in fruit development, and wild tomato species differing in storage sugars serve as an excellent research material for this purpose. Plant vacuolar invertase is a key enzyme of sucrose metabolism in the sink organs. In the present study, we identified complete gene sequences encoding the TAI vacuolar invertase in 11 wild and one cultivated tomato accessions of the Solanum section Lycopersicon...
June 20, 2017: Molecular Genetics and Genomics: MGG
https://www.readbyqxmd.com/read/28621722/comparative-analysis-of-genome-wide-dna-methylation-profiles-for-the-genic-male-sterile-cabbage-line-01-20s-and-its-maintainer-line
#11
Fengqing Han, Xiaoli Zhang, Xing Liu, Henan Su, Congcong Kong, Zhiyuan Fang, Limei Yang, Mu Zhuang, Yangyong Zhang, Yumei Liu, Zhansheng Li, Honghao Lv
Methylation modifications play an important role in multiple biological processes. Several studies have reported altered methylation patterns in male sterile plants such as rice and wheat, but little is known about the global methylation profiles and their possible roles in the cabbage (Brassicaoleracea) male sterile line. In this study, single-base-resolution bisulfite sequencing (BS-Seq) was adopted to identify the pattern and degree of cytosine methylation in the male sterile line 01-20S and its near-isogenic fertile line 01-20F...
June 16, 2017: Genes
https://www.readbyqxmd.com/read/28597466/suppression-of-the-vacuolar-invertase-gene-delays-senescent-sweetening-in-chipping-potatoes
#12
Amy E Wiberley-Bradford, Paul C Bethke
BACKGROUND: Potato chip processors require potato tubers that meet quality specifications for fried chip color, and color depends largely upon tuber sugar contents. At later times in storage, potatoes accumulate sucrose, glucose, and fructose. This developmental process, senescent sweetening, manifests as a blush of color near the center of the fried chip, becomes more severe with time, and limits the storage period. Vacuolar invertase (VInv) converts sucrose to glucose and fructose and is hypothesized to play a role in senescent sweetening...
June 9, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28596758/a-functional-metagenomic-analysis-of-tetracycline-resistance-in-cheese-bacteria
#13
Ana B Flórez, Lucía Vázquez, Baltasar Mayo
Metagenomic techniques have been successfully used to monitor antibiotic resistance genes in environmental, animal and human ecosystems. However, despite the claim that the food chain plays a key role in the spread of antibiotic resistance, metagenomic analysis has scarcely been used to investigate food systems. The present work reports a functional metagenomic analysis of the prevalence and evolution of tetracycline resistance determinants in a raw-milk, blue-veined cheese during manufacturing and ripening...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28591204/feeding-toxicity-and-impact-of-imidacloprid-formulation-and-mixtures-with-six-representative-pesticides-at-residue-concentrations-on-honey-bee-physiology-apis-mellifera
#14
Yu Cheng Zhu, Jianxiu Yao, John Adamczyk, Randall Luttrell
Imidacloprid is the most widely used insecticide in agriculture. In this study, we used feeding methods to simulate in-hive exposures of formulated imidacloprid (Advise® 2FL) alone and mixtures with six representative pesticides for different classes. Advise, fed at 4.3 mg/L (equal to maximal residue detection of 912 ppb active ingredient [a.i.] in pollen) induced 36% mortality and 56% feeding suppression after 2-week feeding. Treatments with individual Bracket (acephate), Karate (λ-cyhalothrin), Vydate (oxamyl), Domark (tetraconazole), and Roundup (glyphosate) at residue level had a mortality range of 1...
2017: PloS One
https://www.readbyqxmd.com/read/28544894/regulation-of-maize-kernel-weight-and-carbohydrate-metabolism-by-abscisic-acid-applied-at-the-early-and-middle-post-pollination-stages-in-vitro
#15
Li Zhang, Xu-Hui Li, Zhen Gao, Si Shen, Xiao-Gui Liang, Xue Zhao, Shan Lin, Shun-Li Zhou
Abscisic acid (ABA) accumulates in plants under drought stress, but views on the role of ABA in kernel formation and abortion are not unified. The response of the developing maize kernel to exogenous ABA was investigated by excising kernels from cob sections at four days after pollination and culturing in vitro with different concentrations of ABA (0, 5, 10, 100μM). When ABA was applied at the early post-pollination stage (EPPS), significant weight loss was observed at high ABA concentration (100μM), which could be attributed to jointly affected sink capacity and activity...
May 10, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28538658/seed-coating-increases-seed-moisture-uptake-and-restricts-embryonic-oxygen-availability-in-germinating-cereal-seeds
#16
Linda Gorim, Folkard Asch
Seed coating is a technology to improve germination and homogenize stand establishment. Although coating often results in lower germination rates, seeds that do germinate grow more vigorously and show strongly reduced respiratory losses during reserve mobilization. We hypothesize that the higher mobilization efficiency is due to a shift in the enzymatic cleavage of sucrose from invertase to sucrose synthase in the embryonic tissue caused by a reduced oxygen availability induced by oversaturation with water caused by the coating during early germination...
May 24, 2017: Biology
https://www.readbyqxmd.com/read/28526983/low-source-sink-ratio-reduces-reserve-starch-in-grapevine-woody-canes-and-modulates-sugar-transport-and-metabolism-at-transcriptional-and-enzyme-activity-levels
#17
Angélica Silva, Henrique Noronha, Zhanwu Dai, Serge Delrot, Hernâni Gerós
Severe leaf removal decreases storage starch and sucrose in grapevine cv. Cabernet Sauvignon fruiting cuttings and modulates the activity of key enzymes and the expression of sugar transporter genes. Leaf removal is an agricultural practice that has been shown to modify vineyard efficiency and grape and wine composition. In this study, we took advantage of the ability to precisely control the number of leaves to fruits in Cabernet Sauvignon fruiting cuttings to study the effect of source-sink ratios (2 (2L), 6 (6L) and 12 (12) leaves per cluster) on starch metabolism and accumulation...
May 19, 2017: Planta
https://www.readbyqxmd.com/read/28501191/tlc-uv-hyphenated-with-maldi-tofms-for-the-screening-of-invertase-substrates-in-plant-extracts
#18
Justine Ferey, David Da Silva, Pierre Lafite, Richard Daniellou, Benoît Maunit
In this study, thin-layer chromatography (TLC) hyphenated with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) was developed for the screening of invertase substrates in complex matrices. BfrA, a specific β-D-fructofuranosidase from Leishmania major, was chosen as a model enzyme to screen biological activity in plant extracts due to its capacity to hydrolyze specific carbohydrates. TLC was considered to be a reliable technique for screening substrates (bioactive molecules) in plant extracts due to its quantitative capabilities whereas MALDI-TOFMS was particularly useful for rapid identification...
August 1, 2017: Talanta
https://www.readbyqxmd.com/read/28480172/high-sucrolytic-activity-by-invertase-immobilized-onto-magnetic-diatomaceous-earth-nanoparticles
#19
Mariana P Cabrera, Caio R D Assis, David F M Neri, Claudete F Pereira, Fernando Soria, Luiz B Carvalho
Invertase immobilized on magnetic diatomaceous earth nanoparticles (mDE-APTES-invertase) with high sucrolytic activity was obtained by an easy and low-cost method. An experimental design was carried out to investigate the best immobilization conditions and it allowed obtaining an immobilized derivative with a residual specific activity equal to 92.5%. Then, a second experimental design selected the mDE-APTES-invertase with higher specific activity in relation to other derivatives reported in the literature (2...
March 2017: Biotechnology Reports
https://www.readbyqxmd.com/read/28467462/synergistic-toxicity-and-physiological-impact-of-imidacloprid-alone-and-binary-mixtures-with-seven-representative-pesticides-on-honey-bee-apis-mellifera
#20
Yu Cheng Zhu, Jianxiu Yao, John Adamczyk, Randall Luttrell
Imidacloprid is the most widely used insecticide in the world. In this study, we used spraying methods to simulate field exposures of bees to formulated imidacloprid (Advise® 2FL) alone and binary mixtures with seven pesticides from different classes. Synergistic toxicity was detected from mixtures of Advise (58.6 mg a.i./L imidacloprid)+Domark (512.5 mg a.i. /L tetraconazole), Advise+Transform (58.5 mg a.i./L sulfoxaflor), and Advise+Vydate (68 mg a.i./L oxamyl), and mortality was significantly increased by 20%, 15%, and 26% respectively...
2017: PloS One
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