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https://www.readbyqxmd.com/read/28211621/changes-in-sugar-content-and-related-enzyme-activities-in-table-grape-vitis-vinifera-l-in-response-to-foliar-selenium-fertilizer
#1
Shuaimeng Zhu, Yinli Liang, Xiaojuan An, Fanchao Kong, Dekai Gao, Hongfei Yin
BACKGROUND: Spraying Se fertilizer is an effective way for Se-enriched fruit production. Sugar content in fruit is the major factor determining berry quality. However, changes in sugar metabolism in response to Se fertilizer are unclear. Hence, this study was conducted to identify the effects of Se fertilizer on sugar metabolism and related enzyme activities of grape berries. Additionally, production of leaves with and without Se fertilizer was also investigated. RESULTS: Acid invertase (AI) activity, total soluble sugar and Se content in berries, photosynthetic rate (Pn) in leaves produced under Se fertilizer treatments were higher than that of control...
February 17, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28204559/suppression-of-extracellular-invertase-inhibitor-gene-expression-improves-seed-weight-in-soybean-glycine-max
#2
Xiaofei Tang, Tao Su, Mei Han, Lai Wei, Weiwei Wang, Zhiyuan Yu, Yongguo Xue, Hongbin Wei, Yejie Du, Steffen Greiner, Thomas Rausch, Lijun Liu
No abstract text is available yet for this article.
January 1, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28194156/integrated-management-strategies-increase-cottonseed-oil-and-protein-production-the-key-role-of-carbohydrate-metabolism
#3
Hongkun Yang, Xinyue Zhang, Binglin Chen, Yali Meng, Youhua Wang, Wenqing Zhao, Zhiguo Zhou
Cottonseed, oil, and protein, as the by-products of cotton production, have the potential to provide commodities to meet the increasing demand of renewable bio-fuels and ruminant feed. An increase in crop yield per unit area requires high-yielding cultivar management with an economic nitrogen (N) rate, an optimal N application schedule, high-yielding plant populations and strong seedlings. Whether the integration of these agronomic practices into a coherent management system can increase the productivity of cotton fiber, embryo oil and protein requires experimental elucidation...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28193587/the-effects-of-rice-straw-biochar-on-indigenous-microbial-community-and-enzymes-activity-in-heavy-metal-contaminated-sediment
#4
Danlian Huang, Linshan Liu, Guangming Zeng, Piao Xu, Chao Huang, Linjing Deng, Rongzhong Wang, Jia Wan
Owning to the potential in carbon sequestration and other environmental benefits, biochar has been widely used for in-situ environmental remediation. Understanding the biological effects of biochar is essential. The goal of this study was to explore the response of indigenous microbes under the stress of different concentrations of biochar. The results showed that biochar could significantly change physicochemical properties, enzymes activity and microbial community composition depending on biochar concentration and incubation time...
January 30, 2017: Chemosphere
https://www.readbyqxmd.com/read/28184245/development-of-a-low-cost-cellulase-production-process-using-trichoderma-reesei-for-brazilian-biorefineries
#5
Simo Ellilä, Lucas Fonseca, Cristiane Uchima, Junio Cota, Gustavo Henrique Goldman, Markku Saloheimo, Vera Sacon, Matti Siika-Aho
BACKGROUND: During the past few years, the first industrial-scale cellulosic ethanol plants have been inaugurated. Although the performance of the commercial cellulase enzymes used in this process has greatly improved over the past decade, cellulases still represent a very significant operational cost. Depending on the region, transport of cellulases from a central production facility to a biorefinery may significantly add to enzyme cost. The aim of the present study was to develop a simple, cost-efficient cellulase production process that could be employed locally at a Brazilian sugarcane biorefinery...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28181575/mycorrhizas-alter-sucrose-and-proline-metabolism-in-trifoliate-orange-exposed-to-drought-stress
#6
Hui-Hui Wu, Ying-Ning Zou, Mohammed Mahabubur Rahman, Qiu-Dan Ni, Qiang-Sheng Wu
Arbuscular mycorrhizal fungi (AMF) can enhance drought tolerance in plants, whereas little is known regarding AMF contribution to sucrose and proline metabolisms under drought stress (DS). In this study, Funneliformis mosseae and Paraglomus occultum were inoculated into trifoliate orange (Poncirus trifoliata) under well watered and DS. Although the 71-days DS notably (P < 0.05) inhibited mycorrhizal colonization, AMF seedlings showed significantly (P < 0.05) higher plant growth performance and leaf relative water content, regardless of soil water status...
February 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28179056/effects-of-nutrient-load-on-microbial-activities-within-a-seagrass-dominated-ecosystem-implications-of-changes-in-seagrass-blue-carbon
#7
Songlin Liu, Zhijian Jiang, Yunchao Wu, Jingping Zhang, Iman Arbi, Feng Ye, Xiaoping Huang, Peter Ian Macreadie
Nutrient loading is a leading cause of global seagrass decline, triggering shifts from seagrass- to macroalgal-dominance. Within seagrass meadows of Xincun Bay (South China Sea), we found that nutrient loading (due to fish farming) increased sediment microbial biomass and extracellular enzyme activity associated with carbon cycling (polyphenol oxidase, invertase and cellulase), with a corresponding decrease in percent sediment organic carbon (SOC), suggesting that nutrients primed microorganism and stimulated SOC remineralization...
February 5, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28155177/cytoplasmic-expression-of-a-thermostable-invertase-from-thermotoga-maritima-in-lactococcus-lactis
#8
Han Bin Pek, Pei Yu Lim, Chengcheng Liu, Dong-Yup Lee, Xuezhi Bi, Fong Tian Wong, Dave Siak-Wei Ow
OBJECTIVES: To evaluate the secretory and cytoplasmic expression of a thermostable Thermogata maritima invertase in Lactococcus lactis. RESULTS: The thermostable invertase from T. maritima was cloned with and without the USP45 secretory peptide into the pNZ8148 vector for nisin-inducible expression in L. lactis. The introduction of an USP45 secretion peptide at the N-terminal of the enzyme led to a loss of protein solubility. Computational homology modeling and hydrophobicity studies indicated that the USP45 peptide exposes a stretch of hydrophobic amino acids on the protein surface resulting in lower solubility...
February 2, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28131340/sucrose-phloem-unloading-follows-an-apoplastic-pathway-with-high-sucrose-synthase-in-actinidia-fruit
#9
Cheng Chen, Yulin Yuan, Chen Zhang, Huixia Li, Fengwang Ma, Mingjun Li
Phloem unloading plays a pivotal role in photoassimilate partitioning and the accumulation of sugars in sink organs, e.g. fruit. Here, we investigated the pathway of sucrose unloading in kiwifruit (Actinidia deliciasa cv. Qinmei) using a combination of electron microscopy, transport of the phloem-mobile symplastic tracer carboxyfluorescein and enzyme activity and gene expression assays. Our structural investigation revealed that the sieve element-companion cell complex of bundles feeding the fruit flesh was symplastically isolated from its surrounding parenchyma cells throughout fruit development, whereas numerous plasmodesmata were present between the phloem parenchyma cells...
February 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28101284/exploring-catalase-and-invertase-activity-using-sodium-alginate-encapsulated-yeast-yeast-spheres
#10
Pamela J Bryer
No abstract text is available yet for this article.
December 2016: Journal of Microbiology & Biology Education: JMBE
https://www.readbyqxmd.com/read/28063146/pretreatment-optimization-of-the-biomass-of-microcystis-aeruginosa-for-efficient-bioethanol-production
#11
Muhammad Imran Khan, Moon Geon Lee, Jin Hyuk Shin, Jong Deog Kim
Microalgae are considered to be the future promising sources of biofuels and bio products. The algal carbohydrates can be fermented to bioethanol after pretreatment process. Efficient pretreatment of the biomass is one of the major requirements for commercialization of the algal based biofuels. In present study the microalga, M. aeruginsa was used for pretreatment optimization and bioethanol production. Treatment of algal biomass with CaO before acid and/or enzymatic hydrolysis enhanced the degradation of algal cells...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28046184/nutritive-value-of-extruded-or-multi-enzyme-supplemented-cold-pressed-soybean-cake-for-pigs
#12
T A Woyengo, R Patterson, C L Levesque
The objectives were to determine the standardized ileal digestibility (SID) of AA and NE value of cold-pressed soybean cake (CP-SBC), and the effect of extrusion or adding multi-enzyme to CP-SBC diet for growing pigs. Eight ileal-cannulated pigs (initial BW = 79.7 ± 3.97 kg) were fed 4 diets in a replicated 4 × 4 Latin square design to give 8 replicates per diet. Diets included a cornstarch-based diet with CP-SBC, extruded CP-SBC, and SBC plus multi-enzyme (1,200 U of xylanase, 150 U of glucanase, 500 U of cellulase, 60 U of mannanase, 700 U of invertase, 5,000 U of protease, and 12,000 U of amylase/kilogram of diet; Superzyme-CS, 0...
December 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/28032636/cytosolic-invertase-contributes-to-the-supply-of-substrate-for-cellulose-biosynthesis-in-developing-wood
#13
Umut Rende, Wei Wang, Madhavi Latha Gandla, Leif J Jönsson, Totte Niittylä
Carbon for cellulose biosynthesis is derived from sucrose. Cellulose is synthesized from uridine 5'-diphosphoglucose (UDP-glucose), but the enzyme(s) responsible for the initial sucrose cleavage and the source of UDP-glucose for cellulose biosynthesis in developing wood have not been defined. We investigated the role of CYTOSOLIC INVERTASEs (CINs) during wood formation in hybrid aspen (Populus tremula × tremuloides) and characterized transgenic lines with reduced CIN activity during secondary cell wall biosynthesis...
December 29, 2016: New Phytologist
https://www.readbyqxmd.com/read/28013473/pollution-in-the-urban-soils-of-lianyungang-china-evaluated-using-a-pollution-index-mobility-of-heavy-metals-and-enzymatic-activities
#14
Yu Li, Hong-Guan Li, Fu-Cheng Liu
Soil samples from 16 urban sites in Lianyungang, China were collected and analyzed. A pollution index was used to assess the potential ecological risk of heavy metals and a sequential extraction procedure was used to evaluate the relative distribution of Cu, Zn, Pb, Cd, Cr, and As in exchangeable, carbonate, Fe/Mn oxide, organic/sulfide, and residual fractions. The mobility of heavy metals and urease (URE) activity, alkaline phosphatase (ALP) activity, and invertase (INV) activity of soils was determined. The results showed that the average concentrations of Cu, Zn, Pb, Cd, Cr, and As in Lianyungang soils were much higher than those in the coastal city soil background values of Jiangsu and China...
January 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28012288/peach-leaf-curl-disease-shifts-sugar-metabolism-in-severely-infected-leaves-from-source-to-sink
#15
Stefano Moscatello, Simona Proietti, Roberto Buonaurio, Franco Famiani, Vittorio Raggi, Robert P Walker, Alberto Battistelli
Peach leaf curl is a disease that affects the leaves of peach trees, and in severe cases all of the leaf can be similarly affected. This study investigated some effects of this disease on the metabolism of peach leaves in which all parts of the leaf were infected. These diseased leaves contained very little chlorophyll and performed little or no photosynthesis. Compared to uninfected leaves, diseased leaves possessed higher contents of fructose and especially glucose, but lowered contents of sucrose, sorbitol and especially starch...
December 1, 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/27994611/linking-expression-of-fructan-active-enzymes-cell-wall-invertases-and-sucrose-transporters-with-fructan-profiles-in-growing-taproot-of-chicory-cichorium-intybus-impact-of-hormonal-and-environmental-cues
#16
Hongbin Wei, Anja Bausewein, Heike Steininger, Tao Su, Hongbo Zhao, Karsten Harms, Steffen Greiner, Thomas Rausch
In chicory taproot, the inulin-type fructans serve as carbohydrate reserve. Inulin metabolism is mediated by fructan active enzymes (FAZYs): sucrose:sucrose 1-fructosyltransferase (1-SST; fructan synthesis), fructan:fructan-1-fructosyltransferase (1-FFT; fructan synthesis and degradation), and fructan 1-exohydrolases (1-FEH1/2a/2b; fructan degradation). In developing taproot, fructan synthesis is affected by source-to-sink sucrose transport and sink unloading. In the present study, expression of FAZYs, sucrose transporter and CWI isoforms, vacuolar invertase and sucrose synthase was determined in leaf blade, petiole and taproot of young chicory plants (taproot diameter: 2 cm) and compared with taproot fructan profiles for the following scenarios: (i) N-starvation, (ii) abscisic acid (ABA) treatment, (iii) ethylene treatment (via 1-aminoyclopropane-1-carboxylic acid [ACC]), and (iv) cold treatment...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27979113/improvement-stability-and-performance-of-invertase-via-immobilization-on-to-silanized-and-polymer-brush-grafted-magnetic-nanoparticles
#17
Gulay Bayramoglu, Tugce Doz, V Cengiz Ozalp, M Yakup Arica
In this study, magnetic nanoparticles (Fe3O4) were modified sequentially with silica (Fe3O4@SiO2), glycidyl methacrylate (GMA) by surface initiated atom transfer radical polymerization (SI-ATRP) and hexamethylene diamine (as a spacer arm). The p(GMA) grafted and SA modified form (i.e., Fe3O4@SiO2@pGMA-SA-3) was used for covalent immobilization of invertase (EC 3.2.1.26). The amount of immobilized enzyme on Fe3O4@SiO2@p(GMA) and Fe3O4@SiO2@p(GMA)-SA-3 was 36.1±0.9 and 33.4±1.3mg/g, respectively. The Km and Vmax values of immobilized invertase were found to be 39...
April 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27965631/long-term-application-of-bioorganic-fertilizers-improved-soil-biochemical-properties-and-microbial-communities-of-an-apple-orchard-soil
#18
Lei Wang, Fang Yang, Yaoyao E, Jun Yuan, Waseem Raza, Qiwei Huang, Qirong Shen
Soil biochemical properties and microbial communities are usually considered as important indicators of soil health because of their association with plant nutrition. In this study, we investigated the impact of long-term application of bioorganic fertilizer (BOF) on soil biochemical properties and microbial communities in the apple orchard soil of the Loess Plateau. The experiment included three treatments: (1) control without fertilization (CK); (2) chemical fertilizer application (CF); and (3) bioorganic fertilizer application (BOF)...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27927993/suppression-of-extracellular-invertase-inhibitor-gene-expression-improves-seed-weight-in-soybean-glycine-max
#19
Xiaofei Tang, Tao Su, Mei Han, Lai Wei, Weiwei Wang, Zhiyuan Yu, Yongguo Xue, Hongbin Wei, Yejie Du, Steffen Greiner, Thomas Rausch, Lijun Liu
Cell wall invertase (CWI) and vacuolar invertase (VI) play multiple functions in plant growth. As well as depending on transcriptional and post-transcriptional regulation, there is growing evidence that CWI and VI are also subject to post-translational control by small inhibitory proteins. Despite the significance of this, genes encoding inhibitors, their molecular and biochemical properties, and their potential roles in regulating seed production have not been well documented in soybean (Glycine max). In this study, two invertase inhibitor isoforms, GmCIF1 and GmC/VIF2, were characterized to possess inhibitory activities in vitro via heterologous expression...
December 7, 2016: Journal of Experimental Botany
https://www.readbyqxmd.com/read/27923989/metabolic-control-of-tobacco-pollination-by-sugars-and-invertases
#20
Marc Goetz, Anne Guivarc'h, Jörg Hirsche, Martin Andreas Bauerfeind, María-Cruz González, Tae Kyung Hyun, Seung Hee Eom, Dominique Chriqui, Thomas Engelke, Dominik K Großkinsky, Thomas Roitsch
Pollination in flowering plants is initiated by germination of pollen grains on stigmas followed by fast growth of pollen tubes representing highly energy-consuming processes. The symplastic isolation of pollen grains and tubes requires import of sucrose available in the apoplast. We show that the functional coupling of sucrose cleavage by invertases and uptake of the released hexoses by monosaccharide transporters are critical for pollination in tobacco. Transcript profiling, in situ hybridization and immunolocalization of extracellular invertases and two monosaccharide transporters in vitro and in vivo support the functional coupling in supplying carbohydrates for pollen germination and tube growth evidenced by spatiotemporally coordinated expression...
December 6, 2016: Plant Physiology
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