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https://www.readbyqxmd.com/read/28416853/changes-in-soluble-sugar-metabolism-in-loquat-fruit-during-different-cold-storage
#1
Yingying Wei, Feng Xu, Xingfeng Shao
To investigate soluble sugar metabolism and its relationship to chilling injury (CI), loquat fruits were stored at 0 and 5 °C for 5 weeks. CI symptoms were monitored during storage, and soluble sugar content and related enzyme activities were measured. Enzymes activities and substrate levels in the ascorbate-glutathione (AsA-GSH) cycle were also determined. Fruit at 0 °C had lower sucrose levels and higher levels of glucose and fructose than fruit maintained at 5 °C, and also manifested less severe CI symptoms...
April 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28415033/physiological-investigation-of-c4-phosphoenolpyruvate-carboxylase-introduced-rice-line-shows-that-sucrose-metabolism-is-involved-in-the-improved-drought-tolerance
#2
Chen Zhang, Xia Li, Yafei He, Jinfei Zhang, Ting Yan, Xiaolong Liu
We compared the drought tolerance of wild-type (WT) and transgenic rice plants (PC) over-expressing the maize C4PEPC gene, which encodes phosphoenolpyruvate carboxylase (PEPC, EC 4.1.1.31) gene, and evaluated the roles of saccharide and sugar-related enzymes in the drought response. Pot-grown seedlings were subjected to real drought conditions outdoors, and the yield components were compared between PC and untransformed wild-type (WT) plants. The stable yield from PC plants was associated with higher net photosynthetic rate under the real drought treatment...
March 30, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28411320/addition-of-straw-from-hyperaccumulator-plants-to-cadmium-contaminated-soil-increases-cadmium-uptake-by-loquat-seedlings
#3
Lijin Lin, Ming'an Liao, Xiulan Lv, Dong Liang, Hui Xia, Jin Wang, Xun Wang
The straw from three different cadmium (Cd) hyperaccumulators (Galinsoga parviflora, Youngia erythrocarpa, and Solanum photeinocarpum) was added to Cd-contaminated soil, and its effects on plant growth and Cd accumulation in loquat seedlings were evaluated. Straw from each of G. parviflora, Y. erythrocarpa, and S. photeinocarpum was added to Cd-contaminated soil before planting seedlings of two varieties of loquat (Dawuxing and Chuanzao). Addition of straw from G. parviflora and S. photeinocarpum increased the root and shoot biomasses of both loquat varieties, compared with that in the control...
May 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28403831/the-role-of-tre6p-and-snrk1-in-maize-early-kernel-development-and-events-leading-to-stress-induced-kernel-abortion
#4
Samuel W Bledsoe, Clémence Henry, Cara A Griffiths, Matthew J Paul, Regina Feil, John E Lunn, Mark Stitt, L Mark Lagrimini
BACKGROUND: Drought stress during flowering is a major contributor to yield loss in maize. Genetic and biotechnological improvement in yield sustainability requires an understanding of the mechanisms underpinning yield loss. Sucrose starvation has been proposed as the cause for kernel abortion; however, potential targets for genetic improvement have not been identified. Field and greenhouse drought studies with maize are expensive and it can be difficult to reproduce results; therefore, an in vitro kernel culture method is presented as a proxy for drought stress occurring at the time of flowering in maize (3 days after pollination)...
April 12, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28371560/differential-scanning-calorimetry-and-fluorimetry-measurements-of-monoclonal-antibodies-and-reference-proteins-effect-of-scanning-rate-and-dye-selection
#5
Brian E Lang, Kenneth D Cole
Differential scanning calorimetry (DSC) and differential scanning fluorimetry (DSF) were used to measure the transition temperatures of four proteins: RNase A, invertase, rituximab, and the NISTmAb (NIST Reference Material, RM 8671). The proteins were combined with several different fluorescent dyes for the DSF measurements. This study compares the results of DSC and DSF measurements of transition temperatures with different types of proteins, dye combinations, and thermal scan rates. As protein unfolding is often influenced by kinetic effects, we measured the transition temperatures of the proteins using DSC over a range of temperature scan rates and compared them to the data obtained from DSF over comparable temperature scan rates...
April 3, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28359854/optimizing-culture-conditions-for-production-of-intra-and-extracellular-inulinase-and-invertase-from-aspergillus-niger-atcc-20611-by-response-surface-methodology-rsm
#6
Mojdeh Dinarvand, Malahat Rezaee, Majid Foroughi
The aim of this study was obtain a model that maximizes growth and production of inulinase and invertase by Aspergillus niger ATCC 20611, employing response surface methodology (RSM). The RSM with a five-variable and three-level central composite design (CCD) was employed to optimize the medium composition. Results showed that the experimental data could be appropriately fitted into a second-order polynomial model with a coefficient of determination (R(2)) more than 0.90 for all responses. This model adequately explained the data variation and represented the actual relationships between the parameters and responses...
February 10, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28349670/metabolic-engineering-to-expand-the-substrate-spectrum-of-pseudomonas-putida-toward-sucrose
#7
Hannes Löwe, Lukas Schmauder, Karina Hobmeier, Andreas Kremling, Katharina Pflüger-Grau
Sucrose is an important disaccharide used as a substrate in many industrial applications. It is a major component of molasses, a cheap by-product of the sugar industry. Unfortunately, not all industrially relevant organisms, among them Pseudomonas putida, are capable of metabolizing sucrose. We chose a metabolic engineering approach to circumvent this blockage and equip P. putida with the activities necessary to consume sucrose. Therefore, we constructed a pair of broad-host range mini-transposons (pSST - sucrose splitting transposon), carrying either cscA, encoding an invertase able to split sucrose into glucose and fructose, or additionally cscB, encoding a sucrose permease...
March 27, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28336973/microbial-community-composition-is-related-to-soil-biological-and-chemical-properties-and-bacterial-wilt-outbreak
#8
Rui Wang, Hongchun Zhang, Liguang Sun, Gaofu Qi, Shu Chen, Xiuyun Zhao
Soil microbes play important roles in plant growth and health. Little is known about the differences of soil microbes between healthy and bacterial wilt infected soils with Ralstonia solanacearum. By Illumina-MiSeq sequencing of 16S rRNA and 18S rRNA gene amplicons, we found the soil microbial composition and diversity were distinct between healthy and bacterial wilt infected soils. Soil microbial community varied at different plant growth stages due to changes of root exudates composition and soil pH. Healthy soils exhibited higher microbial diversity than the bacterial wilt infected soils...
March 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28330196/cloning-and-expression-of-saccharomyces-cerevisiae-suc2-gene-in-yeast-platform-and-characterization-of-recombinant-enzyme-biochemical-properties
#9
Nooshin Mohandesi, Seyed Omid Ranaei Siadat, Kamahldin Haghbeen, Ardeshir Hesampour
Invertase (EC.3.2.1.26) catalyzes the hydrolysis of sucrose to an equimolar mixture of D-glucose and D-fructose which is of interest for various industrial applications. In this research, Saccharomyces cerevisiae invertase gene (SUC2) was optimized based on Pichia pastoris codon preference. The synthetic gene was introduced into the methylotrophic yeast Pichia pastoris under the control of the inducible AOX1 promoter. High level of the extracellular recombinant invertase (R-inv) production was achieved via methanol induction for 4 days and purified by His-Tag affinity chromatography which appeared to be a mixture of glycosylated proteins with various sizes of 85-95 kDa on SDS-PAGE...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28324683/thermal-stress-impacts-reproductive-development-and-grain-yield-in-rice
#10
REVIEW
Muhammad Shakeel Arshad, Muhammad Farooq, Folkard Asch, Jagadish S V Krishna, P V Vara Prasad, Kadambot H M Siddique
Rice is highly sensitive to temperature stress (cold and heat), particularly during the reproductive and grain-filling stages. In this review, we discuss the effects of low- and high-temperature sensitivity in rice at various reproductive stages (from meiosis to grain development) and propose strategies for improving the tolerance of rice to terminal thermal stress. Cold stress impacts reproductive development through (i) delayed heading, due to its effect on anther respiration, which increases sucrose accumulation, protein denaturation and asparagine levels, and decreases proline accumulation, (ii) pollen sterility owing to tapetal hypertrophy and related nutrient imbalances, (iii) reduced activity of cell wall bound invertase in the tapetum of rice anthers, (iv) impaired fertilization due to inhibited anther dehiscence, stigma receptivity and ability of the pollen tube to germinate through the style towards the ovary, and (v) floret sterility, which increases grain abortion, restricts grain size, and thus reduces grain yield...
March 16, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28292708/dioscorea-alata-tuber-proteome-analysis-shows-over-thirty-dioscorin-isoforms-and-novel-tuber-proteins
#11
Shruti Sharma, Ravi Gupta, Renu Deswal
In Dioscorea, dioscorin (31 kDa) is the major storage protein constituting 85% of the total tuber proteins. An integrated proteomic and biochemical approach was used to understand the physiological role of dioscorin in the two contrasting growth stages (germinating and mature tuber). HPLC analysis showed 3 fold reduction in mannitol and 12.88 and 1.24 fold increase in sucrose and maltose in the germinating tuber. A 1.8 and 3 fold increase in sucrose phosphate synthase and mannitol dehydrogenase activity respectively was observed in the germinating tuber while a 2 fold higher invertase probably lowers the sucrose accumulation in the mature tuber...
March 6, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28279167/novel-snp-markers-in-invge-and-sssi-genes-are-associated-with-natural-variation-of-sugar-contents-and-frying-color-in-solanum-tuberosum-group-phureja
#12
Diana Duarte-Delgado, Deissy Juyó, Christiane Gebhardt, Felipe Sarmiento, Teresa Mosquera-Vásquez
BACKGROUND: Potato frying color is an agronomic trait influenced by the sugar content of tubers. The candidate gene approach was employed to elucidate the molecular basis of this trait in Solanum tuberosum Group Phureja, which is mainly diploid and represents an important genetic resource for potato breeding. The objective of this research was to identify novel genetic variants related with frying quality in loci with key functions in carbohydrate metabolism, with the purpose of discovering genetic variability useful in breeding programs...
March 9, 2017: BMC Genetics
https://www.readbyqxmd.com/read/28268040/long-term-liquid-storage-and-reproductive-evaluation-of-an-innovative-boar-semen-extender-formula12-%C3%A2-containing-a-non-reducing-disaccharide-and-an-enzymatic-agent
#13
Carla Bresciani, Annalisa Bianchera, Ruggero Bettini, Annamaria Buschini, Laura Marchi, Clotilde Silvia Cabassi, Alberto Sabbioni, Federico Righi, Claudio Mazzoni, Enrico Parmigiani
There are no reports of saccharolytic enzymes being used in the preparation of formulations for animal semen extenders. In the present study, the use of an innovative semen extender (Formula12(®)) in the long-term liquid storage of boar semen at 17°C was evaluated. The formulation included use of a disaccharide (sucrose) as the energy source precursor coupled to an enzymatic agent (invertase). The innovative extender was evaluated and compared in vitro to a commercial extender (Vitasem LD(®)) for the following variables: Total Motility (TM), Forward Progressive Motility (FPM), sperm morphology, membrane integrity, acrosome integrity, and chromatin instability...
February 24, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28247243/grape-hexokinases-are-involved-in-the-expression-regulation-of-sucrose-synthase-and-cell-wall-invertase-encoding-genes-by-glucose-and-aba
#14
Xiu-Qin Wang, Li-Li Zheng, Hao Lin, Fei Yu, Li-Hui Sun, Li-Mei Li
Hexokinase (HXK, EC 2.7.1.1) is a multifunctional protein that both is involved in catalyzing the first step of glycolysis and plays an important role in sugar signaling. However, the supporting genetic evidence on hexokinases (CsHXKs) from grape (Vitis vinifera L. cv. Cabernet Sauvignon) berries has been lacking. Here, to investigate the role of CsHXK isoforms as glucose (Glc) and abscisic acid (ABA) sensors, we cloned two hexokinase isozymes, CsHXK1 and CsHXK2 with highly conserved genomic structure of nine exons and eight introns...
February 28, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28242903/uv-c-treatment-on-physiological-response-of-potato-solanum-tuberosum-l-during-low-temperature-storage
#15
Qiong Lin, Yajing Xie, Wei Liu, Jie Zhang, Shuzhen Cheng, Xinfang Xie, Wenqiang Guan, Zhidong Wang
The storage of potato tuber (Solanum tuberosum L.) at low temperatures minimizes sprouting and disease but can cause cold-induced sweetening (CIS), which leads to the production of the cancerogenic substance acrylamide during the frying processing. The aim of this research was to investigate the effects of UV-C treatment on CIS in cold stored potato tuber. 'Atlantic' potatoes were treated with UV-C for an hour and then stored at 4 °C up to 28 days. The UV-C treatment significantly prevented the increase of malondialdehyde content (an indicator of the prevention of oxidative injury) in potato cells during storage...
January 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28211621/changes-in-sugar-content-and-related-enzyme-activities-in-table-grape-vitis-vinifera-l-in-response-to-foliar-selenium-fertilizer
#16
Shuaimeng Zhu, Yinli Liang, Xiaojuan An, Fanchao Kong, Dekai Gao, Hongfei Yin
BACKGROUND: Spraying Se fertilizer is an effective way for Se-enriched fruit production. Sugar content in fruit is the major factor determining berry quality. However, changes in sugar metabolism in response to Se fertilizer are unclear. Hence, this study was conducted to identify the effects of Se fertilizer on sugar metabolism and related enzyme activities of grape berries. Additionally, production of leaves with and without Se fertilizer was also investigated. RESULTS: Acid invertase (AI) activity, total soluble sugar and Se content in berries, photosynthetic rate (Pn) in leaves produced under Se fertilizer treatments were higher than that of control...
February 17, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28204559/suppression-of-extracellular-invertase-inhibitor-gene-expression-improves-seed-weight-in-soybean-glycine-max
#17
Xiaofei Tang, Tao Su, Mei Han, Lai Wei, Weiwei Wang, Zhiyuan Yu, Yongguo Xue, Hongbin Wei, Yejie Du, Steffen Greiner, Thomas Rausch, Lijun Liu
No abstract text is available yet for this article.
January 1, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28194156/integrated-management-strategies-increase-cottonseed-oil-and-protein-production-the-key-role-of-carbohydrate-metabolism
#18
Hongkun Yang, Xinyue Zhang, Binglin Chen, Yali Meng, Youhua Wang, Wenqing Zhao, Zhiguo Zhou
Cottonseed, oil, and protein, as the by-products of cotton production, have the potential to provide commodities to meet the increasing demand of renewable bio-fuels and ruminant feed. An increase in crop yield per unit area requires high-yielding cultivar management with an economic nitrogen (N) rate, an optimal N application schedule, high-yielding plant populations and strong seedlings. Whether the integration of these agronomic practices into a coherent management system can increase the productivity of cotton fiber, embryo oil and protein requires experimental elucidation...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28193587/the-effects-of-rice-straw-biochar-on-indigenous-microbial-community-and-enzymes-activity-in-heavy-metal-contaminated-sediment
#19
Danlian Huang, Linshan Liu, Guangming Zeng, Piao Xu, Chao Huang, Linjing Deng, Rongzhong Wang, Jia Wan
Owning to the potential in carbon sequestration and other environmental benefits, biochar has been widely used for in-situ environmental remediation. Understanding the biological effects of biochar is essential. The goal of this study was to explore the response of indigenous microbes under the stress of different concentrations of biochar. The results showed that biochar could significantly change physicochemical properties, enzymes activity and microbial community composition depending on biochar concentration and incubation time...
May 2017: Chemosphere
https://www.readbyqxmd.com/read/28184245/development-of-a-low-cost-cellulase-production-process-using-trichoderma-reesei-for-brazilian-biorefineries
#20
Simo Ellilä, Lucas Fonseca, Cristiane Uchima, Junio Cota, Gustavo Henrique Goldman, Markku Saloheimo, Vera Sacon, Matti Siika-Aho
BACKGROUND: During the past few years, the first industrial-scale cellulosic ethanol plants have been inaugurated. Although the performance of the commercial cellulase enzymes used in this process has greatly improved over the past decade, cellulases still represent a very significant operational cost. Depending on the region, transport of cellulases from a central production facility to a biorefinery may significantly add to enzyme cost. The aim of the present study was to develop a simple, cost-efficient cellulase production process that could be employed locally at a Brazilian sugarcane biorefinery...
2017: Biotechnology for Biofuels
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