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https://www.readbyqxmd.com/read/28928516/enzyme-activity-amino-acid-profiles-and-hydroxymethylfurfural-content-in-ethiopian-monofloral-honey
#1
Abera Belay, Gulelat Desse Haki, Marc Birringer, Hannelore Borck, Young-Chul Lee, Kyung-Tack Kim, Kaleab Baye, Samuel Melaku
The enzymes activity, hydroxymethylfurfural (HMF) and amino acids in honeys are relatively low. However, they play very significant role for honey quality. In this study, enzymes, amino acids and HMF contents of Ethiopian monofloral honeys were investigated. Diastase, invertase and HMF were analyzed based on the Harmonized International Honey Commission method and amino acids using amino acids analyzer (HPLC). Diastase activity ranged from 3.91 ± 0.730 (Schefflera abyssinica) to 13.6 ± 2.30 [Becium grandiflorum (L: Lalibella)]; invertase 36...
August 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28922759/transcriptomic-and-metabolomics-responses-to-elevated-cell-wall-invertase-activity-during-tomato-fruit-set
#2
Lei Ru, Sonia Osorio, Lu Wang, Alisdair R Fernie, John W Patrick, Yong-Ling Ruan
Fruit set is a developmental transition from ovaries to fruitlets that determines yield potential. Cell wall invertase (CWIN) is essential for fruit and seed set, but the underlying molecular basis remains elusive. We addressed this issue by using CWIN-elevated transgenic tomato, focusing on ovaries and fruitlets at 2 d before and after anthesis, respectively. RNAseq analyses revealed that ovaries and fruitlets exhibited remarkable differences in their transcriptomic responses to elevated CWIN activity. Ovaries 2 d before anthesis were far more responsive to elevated CWIN activity compared with the fruitlets...
July 10, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28918636/soluble-protein-and-amino-acid-content-affects-the-foam-quality-of-sparkling-wine
#3
Bruna C Condé, Eloise Bouchard, Julie A Culbert, Kerry L Wilkinson, Sigfredo Fuentes, Kate S Howell
Proteins and amino acids are known to influence the foam characteristics of sparkling wines. However, it is unclear to what extent they promote foam formation and/or stability. This study aimed to investigate the effect of protein content and amino acid composition, measured via the bicinchoninic acid assay and high-performance liquid chromatography respectively, on the foaming properties of 28 sparkling white wines, made by different production methods. Foam volume and stability were determined using a robotic pourer and computer vision algorithms...
September 17, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28902212/switchable-aerobic-anaerobic-multi-substrate-biofuel-cell-operating-on-anodic-and-cathodic-enzymatic-cascade-assemblies
#4
Katharina Herkendell, Ran Tel-Vered, Andreas Stemmer
Enzymatic fuel cells may become more accessible for applications powering portable electronic devices by broadening the range of potentially usable fuels and oxidizers. In this work we demonstrate the operation of an integrated, yet versatile multi-substrate biofuel cell utilizing either glucose, fructose, sucrose or combinations of thereof as biofuels, and molecular oxygen originating from solution phase and/or an internal chemical source, as the oxidizer. In order to achieve this goal we designed an enzymatic cascade-functionalized anode consisting of invertase (INV), mutarotase (MUT), glucose oxidase (GOX), and fructose dehydrogenase (FDH), deposited on top of a mesoporous carbon nanoparticle matrix, in which electron relay molecules had been entrapped...
September 13, 2017: Nanoscale
https://www.readbyqxmd.com/read/28887464/rnaseq-revealed-the-important-gene-pathways-controlling-adaptive-mechanisms-under-waterlogged-stress-in-maize
#5
Kanika Arora, Kusuma Kumari Panda, Shikha Mittal, Mallana Gowdra Mallikarjuna, Atmakuri Ramakrishna Rao, Prasanta Kumar Dash, Nepolean Thirunavukkarasu
Waterlogging causes yield penalty in maize-growing countries of subtropical regions. Transcriptome analysis of the roots of a tolerant inbred HKI1105 using RNA sequencing revealed 21,364 differentially expressed genes (DEGs) under waterlogged stress condition. These 21,364 DEGs are known to regulate important pathways including energy-production, programmed cell death (PCD), aerenchyma formation, and ethylene responsiveness. High up-regulation of invertase (49-fold) and hexokinase (36-fold) in roots explained the ATP requirement in waterlogging condition...
September 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28860154/etiolated-stem-branching-is-a-result-of-systemic-signaling-associated-with-sucrose-level
#6
Bolaji Babajide Salam, Siva Kumar Malka, Xiaobiao Zhu, Huiling Gong, Carmit Ziv, Paula Teper-Bamnolker, Naomi Ori, Jiming Jiang, Dani Eshel
The potato tuber is a swollen stem. Sprouts growing from the tuber nodes represent loss of apical dominance and branching. Long cold storage induces loss of tuber apical dominance and results in secondary branching. Here we show that a similar branching pattern can be induced by short heat treatment of the tubers. Detached sprouts were induced to branch by the heat treatment only when attached to a parenchyma cylinder. Grafting experiments showed that the scion branches only when grafted onto heat- or cold- treated tuber parenchyma, suggesting that the branching signal is transmitted systemically from the bud-base parenchyma to the grafted stem...
August 31, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28822946/differential-expression-of-vacuolar-and-defective-cell-wall-invertase-genes-in-roots-and-seeds-of-metalliferous-and-non-metalliferous-populations-of-rumex-dentatus-under-copper-stress
#7
Zhong-Rui Xu, Shen-Wen Cai, Wu-Xing Huang, Rong-Xiang Liu, Zhi-Ting Xiong
Acid invertase activities in roots and young seeds of a metalliferous population (MP) of Rumex dentatus were previously observed to be significantly higher than those of a non-metalliferous population (NMP) under Cu stress. To date, no acid invertase gene has been cloned from R. dentatus. Here, we isolated four full-length cDNAs from the two populations of R. dentatus, presumably encoding cell wall (RdnCIN1 and RdmCIN1 from the NMP and MP, respectively) and vacuolar invertases (RdnVIN1 and RdmVIN1 from the NMP and MP, respectively)...
August 17, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28816436/integrated-distance-based-origami-paper-analytical-device-for-one-step-visualized-analysis
#8
Tian Tian, Yuan An, Yiping Wu, Yanling Song, Zhi Zhu, Chaoyong Yang
An integrated distance-based origami paper analytical device (ID-oPAD) is developed for simple, user friendly and visual detection of targets of interest. The platform enables complete integration of target recognition, signal amplification, and visual signal output based on aptamer/invertase-functionalized sepharose beads, cascaded enzymatic reactions, and a 3D microfluidic paper-based analytical device with distance-based readout, respectively. The invertase-DNA conjugate is released upon target addition, after which it permeates through the cellulose and flows down into the bottom detection zone, whereas sepharose beads with larger size are excluded and stay in the upper zone...
September 13, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28810218/effect-of-cold-adapted-microbial-agent-inoculation-on-enzyme-activities-during-composting-start-up-at-low-temperature
#9
Qinghong Sun, Di Wu, Zhechao Zhang, Yue Zhao, Xinyu Xie, Junqiu Wu, Qian Lu, Zimin Wei
In order to put forward a method to promote composting start-up at low ambient temperature, the cold-adapted microbial agent (CAMA) was inoculated in chicken manure (CM), and compared the enzymes activities, including urease, proteases, β-glucosidase and invertase, with no CAMA group (CK). In this study, the temperature of CM reached 50°C in 53h, but it in CK was only around 30°C during the composting process. Moreover, the enzymes exhibited higher activity in CM than CK, indicating the effectiveness of CAMA...
November 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28798491/phytochromobilin-deficiency-impairs-sugar-metabolism-through-the-regulation-of-cytokinin-and-auxin-signaling-in-tomato-fruits
#10
Ricardo Ernesto Bianchetti, Aline Bertinatto Cruz, Bruna Soares Oliveira, Diego Demarco, Eduardo Purgatto, Lázaro Eustáquio Pereira Peres, Magdalena Rossi, Luciano Freschi
Phytochomes and plant hormones have been emerging as important regulators of fleshy fruit biology and quality traits; however, the relevance of phytochrome-hormonal signaling crosstalk in controlling fruit development and metabolism remains elusive. Here, we show that the deficiency in phytochrome chromophore phytochromobilin (PΦB) biosynthesis inhibits sugar accumulation in tomato (Solanum lycopersicum) fruits by transcriptionally downregulating sink- and starch biosynthesis-related enzymes, such as cell-wall invertases, sucrose transporters and ADP-glucose pyrophosphorylases...
August 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28744865/a-unique-invertase-is-important-for-sugar-absorption-of-an-obligate-biotrophic-pathogen-during-infection
#11
Qing Chang, Jie Liu, Xiaohong Lin, Shoujun Hu, Yang Yang, Dan Li, Liyang Chen, Baoyu Huai, Lili Huang, Ralf T Voegele, Zhensheng Kang
An increased invertase activity in infected plant tissue has been observed in many plant-pathogen interactions. However, the origin of this increased invertase activity (plant and/or pathogen) is still under debate. In addition, the role of pathogen invertases in the infection process is also unclear. We identified and cloned a gene with homology to invertases from Puccinia striiformis f. sp. tritici (Pst). Transcript levels of PsINV were analyzed by quantitative reverse transcription PCR in both compatible and incompatible Pst-wheat interactions ...
September 2017: New Phytologist
https://www.readbyqxmd.com/read/28740313/characterization-of-portuguese-honey-from-castelo-branco-region-according-to-their-pollen-spectrum-physicochemical-characteristics-and-mineral-contents
#12
Luís R Silva, Adriana Sousa, Marcos Taveira
The aim of this work was to evaluate the quality of 16 honey samples from Castelo Branco region (Portugal). Twelve are monofloral from Calluna vulgaris, Erica, Lavandula, Echium and Campanula. The mean values obtained for physicochemical parameters were: 3.82 pH; 16.80% moisture; 81.6°Brix; 0.21% ash; 357.6 μS cm(-1) electrical conductivity; 33.7 meq/kg free acidity; 5.7 meq/kg lactonic acidity; 39.3 meq/kg total acidity; 9.11 mg/kg HMF; 21.3 IN invertase and 9.0° Gothe for diastase activities. The results indicate a good quality, adequate processing, good maturity and freshness of honey...
July 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28731585/bacterial-invertases-occurrence-production-biochemical-characterization-and-significance-of-transfructosylation
#13
REVIEW
Lynette Lincoln, Sunil S More
Invertase or β-D-fructofuranoside fructohydrolase (EC 3.2.1.26) was one of the foremost enzyme biocatalysts and established the primary concepts of most enzyme-kinetic principles. Invertases are glycoside hydrolases and occur mostly in microorganisms. Among microbial strains, for many decades yeast species have been extensively researched for invertase production, characterization, and applications in industries. Besides, limited literature is available on invertases from bacterial strains. The enzymic and molecular biological reports from bacterial invertases are scarce...
July 21, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28717209/non-target-effects-on-soil-microbial-parameters-of-the-synthetic-pesticide-carbendazim-with-the-biopesticides-cantharidin-and-norcantharidin
#14
Hainan Shao, Yalin Zhang
Considering the fact that biopesticides are increasingly used to replace synthetic pesticides in pest control, it is necessary to assess their ecotoxicity and especially their non-target effects on soil microorganisms, which is largely unknown. In this study, the effects of the synthetic pesticide carbendazim and the biopesticides (cantharidin and norcantharidin) on soil microbial parameters in a silt loam soil were evaluated. By using commercial formulations at the recommended and higher rates, both cantharidin and norcantharidin induced adverse effects on soil invertase, phosphatase activities and fungal gene structure, but these changes were transient...
July 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28694802/the-response-regulator-slr1588-regulates-spsa-but-is-not-crucial-for-salt-acclimation-of-synechocystis-sp-pcc-6803
#15
Kuo Song, Martin Hagemann, Xiaoming Tan, Xuefeng Lu
Cyanobacterial sucrose biosynthesis is stimulated under salt stress, which could be used for biotechnological sugar production. It has been shown that the response regulator Slr1588 negatively regulates the spsA gene encoding sucrose-phosphate synthase and mutation of the slr1588 gene also affected the salt tolerance of Synechocystis (Chen et al., 2014). The latter finding is contrary to earlier observations (Hagemann et al., 1997b). Here, we observed that ectopic expression of slr1588 did not restore the salt tolerance of the slr1588 mutant, making the essential function of this response regulator for salt tolerance questionable...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28690616/comparative-transcriptome-analysis-reveals-critical-function-of-sucrose-metabolism-related-enzymes-in-starch-accumulation-in-the-storage-root-of-sweet-potato
#16
Kai Zhang, Zhengdan Wu, Daobin Tang, Kai Luo, Huixiang Lu, Yingying Liu, Jie Dong, Xin Wang, Changwen Lv, Jichun Wang, Kun Lu
The starch properties of the storage root (SR) affect the quality of sweet potato (Ipomoea batatas (L.) Lam.). Although numerous studies have analyzed the accumulation and properties of starch in sweet potato SRs, the transcriptomic variation associated with starch properties in SR has not been quantified. In this study, we measured the starch and sugar contents and analyzed the transcriptome profiles of SRs harvested from sweet potatoes with high, medium, and extremely low starch contents, at five developmental stages [65, 80, 95, 110, and 125 days after transplanting (DAP)]...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28671003/effective-antigen-delivery-via-dual-entrapment-in-erythrocytes-and-autologous-plasma-beads
#17
Munazza Tamkeen Fatima, Ejaj Ahmad, Mehboob Hoque
Fibrin-based polymeric systems have now emerged as efficient carriers of drugs, growth factors, genes, and cells. Earlier, we have reported fibrin based plasma beads (PB), prepared from clotted whole plasma, as an efficient system for the controlled release of entrapped therapeutics. In the present study, we investigate the dual entrapment in erythrocytes and PB, as potential particulate antigen delivery system in rabbit and mice, with yeast invertase as the model antigen. Preparations used for immunization include- invertase entrapped in erythrocytes, the same further entrapped in PB, and crosslinked with glutaraldehyde...
July 3, 2017: Journal of Drug Targeting
https://www.readbyqxmd.com/read/28658609/postharvest-changes-in-lin5-down-regulated-plants-suggest-a-role-for-sugar-deficiency-in-cuticle-metabolism-during-ripening
#18
José G Vallarino, Trevor H Yeats, Eugenia Maximova, Jocelyn K Rose, Alisdair R Fernie, Sonia Osorio
The cell wall invertase gene (LIN5) was reported to be a key enzyme influencing sugar uptake of tomato (Solanum lycopersicum) fruit. It was additionally revealed to be a key regulator of total soluble solids content in fruit as well as for reproductive development, being mainly involved in flower development, early fruit and seed development but also in ripening. Here, we demonstrate that silencing of the LIN5 gene promotes changes affecting fruit cuticle development which has a direct effect on postharvest properties...
October 2017: Phytochemistry
https://www.readbyqxmd.com/read/28651190/temporal-dynamics-of-the-compositions-and-activities-of-soil-microbial-communities-post-application-of-the-insecticide-chlorantraniliprole-in-paddy-soils
#19
Meng Wu, Jia Liu, Weitao Li, Ming Liu, Chunyu Jiang, Zhongpei Li
Chlorantraniliprole (CAP) is a newly developed insecticide widely used in rice fields in China. There has been few studies evaluating the toxicological effects of CAP on soil-associated microbes. An 85-day microcosm experiment was performed to reveal the dissipation dynamics of CAP in three types of paddy soils in subtropical China. The effects of CAP on microbial activities (microbial biomass carbon-MBC, basal soil respiration-BSR, microbial metabolic quotient-qCO2, acid phosphatase and sucrose invertase activities) in the soils were periodically evaluated...
June 23, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28645264/molecular-cloning-structure-phylogeny-and-expression-analysis-of-the-invertase-gene-family-in-sugarcane
#20
Liming Wang, Yuexia Zheng, Shihui Ding, Qing Zhang, Youqiang Chen, Jisen Zhang
BACKGROUND: Invertases (INVs) are key enzymes regulating sucrose metabolism and are here revealed to be involved in responses to environmental stress in plants. To date, individual members of the invertase gene family and their expression patterns are unknown in sugarcane due to its complex genome despite their significance in sucrose metabolism. RESULTS: In this study, based on comparative genomics, eleven cDNA and twelve DNA sequences belonging to 14 non-redundant members of the invertase gene family were successfully cloned from sugarcane...
June 23, 2017: BMC Plant Biology
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