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https://www.readbyqxmd.com/read/29355554/dual-inhibition-of-hy023016-based-on-binding-properties-of-platelet-membrane-receptor-subunit-glycoprotein-ib%C3%AE-and-thrombin-exosites
#1
Qiu-Fang Chen, Shuang Cui, Hui-Liang Shen, Xiang Chen, Yun-Zhan Li, Qian Wu, Yun-Gen Xu, Guo-Qing Gong
Thrombin has long been suggested as a desirable antithrombotic target, but anti-thrombin therapy without anti-platelet thereby has never achieved the ideal effect. HY023016 is a novel compound, in our previous study, it exerted better anti-thrombotic than dabigatran etexilate. The present study aims to illustrate the excess anti-thrombotic molecular mechanisms of HY023016 through thrombin anion exosites and the platelet membrane receptor subunit glycoprotein Ibα (GPIbα). HY023016 strongly inhibited the conversion of fibrinogen to fibrous may via blocking thrombin exosite I...
January 17, 2018: European Journal of Pharmacology
https://www.readbyqxmd.com/read/29352288/phospholipid-scramblase-1-interacts-with-influenza-a-virus-np-impairing-its-nuclear-import-and-thereby-suppressing-virus-replication
#2
Weiyu Luo, Jie Zhang, Libin Liang, Guangwen Wang, Qibing Li, Pengyang Zhu, Yuan Zhou, Junping Li, Yuhui Zhao, Nan Sun, Shanyu Huang, Chenchen Zhou, Yu Chang, Pengfei Cui, Pucheng Chen, Yongping Jiang, Guohua Deng, Zhigao Bu, Chengjun Li, Li Jiang, Hualan Chen
Transcription and replication of the influenza A virus (IAV) genome occur in the nucleus of infected cells and are carried out by the viral ribonucleoprotein complex (vRNP). As a major component of the vRNP complex, the viral nucleoprotein (NP) mediates the nuclear import of the vRNP complex via its nuclear localization signals (NLSs). Clearly, an effective way for the host to antagonize IAV infection would be by targeting vRNP nuclear import. Here, we identified phospholipid scramblase 1 (PLSCR1) as a binding partner of NP by using a yeast two-hybrid (Y2H) screen...
January 19, 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29351796/the-jun-mir-22-hur-regulatory-axis-contributes-to-tumourigenesis-in-colorectal-cancer
#3
Yanqing Liu, Xiaorui Chen, Rongjie Cheng, Fei Yang, Mengchao Yu, Chen Wang, Shufang Cui, Yeting Hong, Hongwei Liang, Minghui Liu, Chihao Zhao, Meng Ding, Wu Sun, Zhijian Liu, Feng Sun, Chenyu Zhang, Zhen Zhou, Xiaohong Jiang, Xi Chen
BACKGROUND: Colorectal cancer (CRC) is a severe health problem worldwide. Clarifying the mechanisms for the deregulation of oncogenes and tumour suppressors in CRC is vital for its diagnosis, treatment, prognosis and prevention. Hu antigen R (HuR), which is highly upregulated in CRC, functions as a pivotal oncogene to promote CRC progression. However, the underlying cause of its dysregulation is poorly understood. METHODS: In CRC tissue sample pairs, HuR protein levels were measured by Western blot and immunohistochemical (IHC) staining, respectively...
January 19, 2018: Molecular Cancer
https://www.readbyqxmd.com/read/29350287/long-non-coding-rna-h19-promotes-glucose-metabolism-and-cell-growth-in-malignant-melanoma-via-mir-106a-5p-e2f3-axis
#4
Wenkang Luan, Zhou Zhou, Xin Ni, Yun Xia, Jinlong Wang, Yulan Yan, Bin Xu
PURPOSE: lncRNA H19 has been considered as an oncogenic lncRNA in many human tumours. In the present study, we identify the role and molecular mechanism of lncRNA H19 in melanoma. METHOD: QRT-PCR was used to detect the expression of lncRNA H19 and E2F3 was detected in melanoma tissues. Cell counting kit-8 (CCK8), representative metabolites analysis was used to explore the biological function of lncRNA H19, miR-106a-5p and E2F3 in melanoma cells. Bioinformatics, luciferase reporter assays, MS2-RIP and RNA pull-down assay was used to demonstrate the molecular mechanism of lncRNA H19 in melanoma...
January 19, 2018: Journal of Cancer Research and Clinical Oncology
https://www.readbyqxmd.com/read/29348174/oligomer-formation-and-g-quadruplex-binding-by-purified-murine-rif1-protein-a-key-organizer-of-higher-order-chromatin-architecture
#5
Kenji Moriyama, Naoko Yoshizawa-Sugata, Hisao Masai
Rap1-interacting protein 1(Rif1) regulates telomere length in budding yeast. We previously reported that, in metazoans and fission yeast, Rif1 also plays pivotal roles in controlling genome-wide DNA replication timing. We proposed that Rif1 may assemble chromatin compartments that contain specific replication-timing domains by promoting chromatin loop formation. Rif1 also is involved in DNA lesion repair, restart after replication fork collapse, anti-apoptosis activities, replicative senescence, and transcriptional regulation...
January 18, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29343573/porcine-mx1-protein-inhibits-classical-swine-fever-virus-replication-by-targeting-nonstructural-protein-ns5b
#6
Jing Zhou, Jing Chen, Xiao-Min Zhang, Zhi-Can Gao, Chun-Chun Liu, Yun-Na Zhang, Jin-Xiu Hou, Zhao-Yao Li, Lin Kan, Wen-Liang Li, Bin Zhou
Mx proteins are interferon-induced GTPases that have broad antiviral activity against a wide range of RNA and DNA viruses; they belong to the dynamin superfamily of large GTPases. In this study, we confirmed the anti-CSFV activity of porcine Mx1 in vitro and showed that porcine Mx2 (poMx2), human MxA (huMxA), and mouse Mx1 (mmMx1) also have anti-CSFV activity in vitro. siRNA experiments revealed that depletion of endogenous poMx1 or poMx2 enhanced CSFV replication, suggesting that porcine Mx proteins are responsible for the antiviral activity of interferon-α (IFNα) against CSFV infection...
January 17, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29338713/lncrna-dancr-involved-osteolysis-after-total-hip-arthroplasty-by-regulating-foxo1-expression-to-inhibit-osteoblast-differentiation
#7
Zhenyu Tang, Zongming Gong, Xiaoliang Sun
BACKGROUND: Aseptic loosening of artificial hip joint is a major complication affecting the long-term use of the artificial hip joint, and is the main cause of joint replacement failure. However, the mechanism of aseptic loosening of THR has not yet cleared. The aim of this study was to investigate the underlying mechanism of DANCR in osteoblast differentiation (OD). METHODS: We detected the expressions of DANCR and FOXO1 in clinical samples and mesenchymal stem cells (MSCs) by qRT-PCR and western blotting...
January 16, 2018: Journal of Biomedical Science
https://www.readbyqxmd.com/read/29335257/staphylococcal-superantigens-use-lama2-as-a-coreceptor-to-activate-t-cells
#8
Zhigang Li, Joseph J Zeppa, Mark A Hancock, John K McCormick, Terence M Doherty, Geoffrey N Hendy, Joaquín Madrenas
Canonical Ag-dependent TCR signaling relies on activation of the src-family tyrosine kinase LCK. However, staphylococcal superantigens can trigger TCR signaling by activating an alternative pathway that is independent of LCK and utilizes a Gα11-containing G protein-coupled receptor (GPCR) leading to PLCβ activation. The molecules linking the superantigen to GPCR signaling are unknown. Using the ligand-receptor capture technology LRC-TriCEPS, we identified LAMA2, the α2 subunit of the extracellular matrix protein laminin, as the coreceptor for staphylococcal superantigens...
January 15, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29333942/six1-and-dach1-influence-the-proliferation-and-apoptosis-of-hepatocellular-carcinoma-through-regulating-p53
#9
Qi Cheng, Deng Ning, Jin Chen, Xue Li, Xiaoping Chen, Li Jiang
ABSTRACTS This research aimed to explore effects of SIX1 and DACH1 on hepatocellular carcinoma (HCC) cell proliferation, apoptosis and cell cycle. Fifty paired hepatocellular carcinoma tissues were screened for differentially expressed genes. SIX1 and DACH1 expressions were subjected to qRT-PCR and western blot in tumor tissues and cells. The knockdown efficiency of siRNAs and transfection efficiency of cDNAs and siRNAs were validated by qRT-PCR and western blot as well. Then colony formation assay and flow cytometry were applied to observe cell proliferation, cell apoptosis and cell cycle changes...
January 15, 2018: Cancer Biology & Therapy
https://www.readbyqxmd.com/read/29333768/-effects-of-geranylgeranyltransferase-%C3%A2-gene-silencing-by-rna-interference-on-the-migration-and-invasion-of-tongue-carcinoma
#10
Chen Zhenggang, Wang Shuren, Li Jinghua, Han Jinhong, Wang Qimin, Tong Lei, Liu Wenjun, Yang Fang, Guo Qingyuan, Guo Dawei, Wang Ying
OBJECTIVE: RNA interference was used to silence geranylgeranyltransferase Ⅰ(GGTase-Ⅰ) in vitro and to study the effect of GGTase-Ⅰ on the migration and invasion of tongue squamous cancer cells. METHODS: Three small interfering RNAs (siRNA) were designed according to the GGTase-Ⅰ sequence by Genebank and were transfected into tongue squamous cancer cells Cal-27 to knock down GGTase-Ⅰ expression. The tested cells were divided into three groups, as follows: the RNA-interfered groups (GGTase-Ⅰ siRNA1, GGTase-Ⅰ siRNA 2, GGTase-Ⅰ siRNA 3), a negative control group (disrupted by random sequence NC-siRNA), and a blank control group...
December 1, 2017: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/29332792/silver-ions-enhanced-auncs-fluorescence-as-a-turn-off-nanoprobe-for-ultrasensitive-detection-of-iodide
#11
Wenli Hou, Yuan Chen, Qiujun Lu, Meiling Liu, Youyu Zhang, Shouzhuo Yao
Fluorescence nanoprobes are frequently employed to construct sensitive biosensors via turn-on and turn-off strategy. In this paper, a novel strategy for ultrasensitive detection of iodide was firstly constructed based on Ag+ regulated photoluminescence enhancement of gold nanoclusters (AuNCs) as a turn-off nanoplatform. In the presence of Ag+, the fluorescence (FL) intensity of AuNCs can be enhanced obviously. When adding iodide ions (I-) in the Ag+-AuNCs, Ag+ can be pulled down from AuNCs and results in quenching of the fluorescent effectively owing to the combination between Ag+ and I-...
April 1, 2018: Talanta
https://www.readbyqxmd.com/read/29329424/brassinosteroids-regulate-pavement-cell-growth-by-mediating-bin2-induced-microtubule-stabilization
#12
Xiaolei Liu, Qin Yang, Yuan Wang, Linhai Wang, Ying Fu, Xuelu Wang
Brassinosteroids (BRs), a group of plant steroid hormones, play important roles in regulating plant development. The cytoskeleton also affects key developmental processes and a deficiency in BR biosynthesis or signaling leads to abnormal phenotypes similar to those of microtubule-defective mutants. However, how BRs regulate microtubule and cell morphology remains unknown. Here, using liquid chromatography-tandem mass spectrometry, we identified tubulin proteins that interact with Arabidopsis BRASSINOSTEROID INSENSITIVE2 (BIN2), a negative regulator of BR responses in plants...
January 10, 2018: Journal of Experimental Botany
https://www.readbyqxmd.com/read/29328525/a-role-of-sialic-acid-containing-glycans-of-matrix-metalloproteinase-9-mmp-9-in-the-interaction-between-mmp-9-and-staphylococcal-superantigen-like-protein-5-ssl5
#13
Chisato Kurisaka, Teruaki Oku, Saotomo Itoh, Tsutomu Tsuji
The staphylococcal superantigen-like proteins (SSLs) exhibit no superantigenic activity but have recently been considered to act as immune suppressors. It was previously reported that SSL5 bound to P-selectin glycoprotein ligand-1 (PSGL-1) and matrix metalloproteinase (MMP)-9, leading to inhibition of leukocyte adhesion and invasion. These interactions were suggested to depend on sialic acid-containing glycans of MMP-9, but the roles of sialic acids in the interaction between SSL5 and MMP-9 are still controversial...
January 12, 2018: Microbiology and Immunology
https://www.readbyqxmd.com/read/29328469/human-cytomegalovirus-ul141-protein-interacts-with-celf5-and-affects-viral-dna-replication
#14
Fei Zou, Zhi-Tao Lu, Shuang Wang, Si Wu, Ying-Ying Wu, Zheng-Rong Sun
Human cytomegalovirus (HCMV) infection is the primary viral cause of congenital abnormalities and mental retardation in newborns. The HCMV UL141‑encoded glycoprotein has been previously revealed to inhibit the cell‑surface expression of cluster of differentiation (CD)155, CD122, tumor necrosis factor‑related apoptosis‑inducing ligand death (TRAIL)‑receptor 1 (R1) and TRAIL‑receptor 2 (R2), thus protecting virally‑infected cells by allowing them to escape natural killer cell‑mediated cytotoxicity...
January 10, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29319165/long-noncoding-rna-neat1-regulate-papillary-thyroid-cancer-progression-by-modulating-mir-129-5p-klk7-expression
#15
Hong Zhang, Yuechang Cai, Li Zheng, Zhanlei Zhang, Xiaofeng Lin, Ningyi Jiang
PURPOSE: Considering the dilemma in papillary thyroid cancer treatment, this study intended to find solution in molecular respect. By probing into lncRNA-NEAT1/miR-129-5p/ KLK7 interaction, this study would provide new targets for future treatment. METHODS: Microarray analysis and R language package were applied to select possible lncRNA and miRNA. Luciferase reporter assay and RNA pull-down test were employed in the detection of target relationship between lncRNA and miRNA...
January 10, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29317724/rfx1-and-rfx3-transcription-factors-interact-with-the-d-sequence-of-adeno-associated-virus-inverted-terminal-repeat-and-regulate-aav-transduction
#16
Laura Julien, Julie Chassagne, Cécile Peccate, Stéphanie Lorain, France Piétri-Rouxel, Olivier Danos, Sofia Benkhelifa-Ziyyat
Adeno-associated virus (AAV) transduction efficiency depends on the way in which cellular proteins process viral genomes in the nucleus. In this study, we have investigated the binding of nuclear proteins to the double stranded D (dsD) sequence of the AAV inverted terminal repeat (ITRs) by electromobility shift assay. We present here several lines of evidence that transcription factors belonging to the RFX protein family bind specifically and selectively to AAV2 and AAV1 dsD sequences. Using supershift experiments, we characterize complexes containing RFX1 homodimers and RFX1/RFX3 heterodimers...
January 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29317594/polymorphism-in-tmem132d-regulates-expression-and-anxiety-related-behavior-through-binding-of-rna-polymerase-ii-complex
#17
Roshan R Naik, Sergey V Sotnikov, Rebekka P Diepold, Stella Iurato, Patrick O Markt, Andrea Bultmann, Nadine Brehm, Tobias Mattheus, Beat Lutz, Angelika Erhardt, Elisabeth B Binder, Ulrike Schmidt, Florian Holsboer, Rainer Landgraf, Ludwig Czibere
TMEM132D is a candidate gene, where risk genotypes have been associated with anxiety severity along with higher mRNA expression in the frontal cortex of panic disorder patients. Concurrently, in a high (HAB) and low (LAB) trait anxiety mouse model, Tmem132d was found to show increased expression in the anterior cingulate cortex (aCC) of HAB as compared to LAB mice. To understand the molecular underpinnings underlying the differential expression, we sequenced the gene and found two single-nucleotide polymorphisms (SNPs) in the promoter differing between both lines which could explain the observed mRNA expression profiles using gene reporter assays...
January 10, 2018: Translational Psychiatry
https://www.readbyqxmd.com/read/29312625/long-non-coding-rna-hoxa11-as-promotes-the-proliferation-hcc-cells-by-epigenetically-silencing-dusp5
#18
Bin Liu, Jing Li, Xiaoling Liu, Min Zheng, Ye Yang, Qian Lyu, Li Jin
Hepatocellular carcinoma has been identified as the fifth most common cancer in men and the ninth in women worldwide. Despite many efforts have been made in recent years, the overall survival rate of patients with hepatocellular carcinoma still remain unsatisfied. Therefore, exploring the mechanisms underlying the progression of hepatocellular carcinoma is essential for developing novel treatments to improve patient prognosis. HOXA11-AS, transcribed from the opposite strand of the protein-coding gene HOXA11, has been identified to be associated with the malignant characteristics of several cancers...
December 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/29312607/androgen-enhances-the-activity-of-ets-1-and-promotes-the-proliferation-of-hcc-cells
#19
Hui Ren, Bo Ren, Jiabin Zhang, Xiaofeng Zhang, Lixin Li, Lingzhan Meng, Zhijie Li, Jia Li, Yinjie Gao, Xuemei Ma
The expression of androgen receptor (AR) has been detected in hepatocellular cancer (HCC). However, there is no universal model detailing AR's function and mechanism in HCC. This study's results show that treatment with dihydrotestosterone (DHT), an endogenous androgen, promoted HCC cells' proliferation and up-regulated the transcription factor activity of ETS-1 (E26 transformation specific sequence 1), which mediates the migration and invasion of cancer cells via protein-protein interaction between AR and ETS-1...
December 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/29305511/a-cytoplasmic-antiholin-is-embedded-in-frame-with-the-holin-in-a-lactobacillus-fermentum-bacteriophage
#20
Tingting Guo, Yongping Xin, Chenchen Zhang, Jian Kong
In double-stranded DNA bacteriophages, infection cycles are ended by host cell lysis through the action of phage-encoded endolysins and holins. The precise timing of lysis is regulated by the holin inhibitors, named antiholins. Sequence analysis has revealed that holins with a single transmembrane domain (TMD) are prevalent in Lactobacillus bacteriophages. A temperate bacteriophage of Lbfermentum, ϕPYB5, has a two-component lysis cassette containing endolysin Lyb5 and holin Hyb5. The hyb5 gene is 465-bp long, encoding 154 amino acid residues with an N-terminal TMD and a large cytoplasmic C-terminal domain...
January 5, 2018: Applied and Environmental Microbiology
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