keyword
MENU ▼
Read by QxMD icon Read
search

Pull down assay

keyword
https://www.readbyqxmd.com/read/29785832/lectin-microarray-combined-with-mass-spectrometry-identifies-haptoglobin-related-protein-hpr-as-a-potential-serologic-biomarker-for-separating-non-bacterial-pneumonia-from-bacterial-pneumonia-in-childhood
#1
Lin Yang, Zhou Yang, Lei Cheng, Juan Cheng, Li Cheng, Yangyang Sun, Wenting Li, Kai Song, Weichun Huang, Yong Yin, Shengce Tao, Qinghua Zhang
PURPOSE: Etiological diagnosis of pediatric patients with community-acquired pneumonia is difficult. For therapy, one of the major problems is the difficulty in separating bacterial pneumonia which would benefit from antibiotics from non-bacterial pneumonia. Therefore, we sought to identify potential biomarkers for distinguishing non-bacterial pneumonia from bacterial pneumonia. EXPERIMENTAL DESIGN: Lectin microarray containing 91 lectins was used to screen serums from pediatric patients with pneumonia...
May 22, 2018: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/29782545/rosmarinic-acid-is-a-novel-inhibitor-for-hepatitis-b-virus-replication-targeting-viral-epsilon-rna-polymerase-interaction
#2
Yuta Tsukamoto, Sotaro Ikeda, Koji Uwai, Riho Taguchi, Kazuaki Chayama, Takemasa Sakaguchi, Ryo Narita, Wan-Ling Yao, Fumihiko Takeuchi, Yukie Otakaki, Koichi Watashi, Takaji Wakita, Hiroki Kato, Takashi Fujita
Current therapeutics for hepatitis B virus (HBV) patients such as nucleoside analogs (NAs) are effective; however, new antiviral drugs against HBV are still desired. Since the interaction between the epsilon (ε) sequence of HBV pregenomic RNA and viral polymerase (Pol) is a key step in the HBV replication cycle, we aimed to identify small compounds for its inhibition, and established a pull-down assay system for the detection of ε-RNA-binding-Pol. Screening showed that 5 out of 3,965 compounds inhibited ε-Pol binding, and we identified rosmarinic acid, which exhibited specificity, as a potential antiviral agent...
2018: PloS One
https://www.readbyqxmd.com/read/29775758/lncrna-prncr1-regulates-osteogenic-differentiation-in-osteolysis-after-hip-replacement-by-targeting-mir-211-5p
#3
Zong-Ming Gong, Zhen-Yu Tang, Xiao-Liang Sun
BACKGROUND: Osteogenic differentiation and osteolysis after hip replacement are both associated with bone metabolism. Interaction between lncRNA PRNCR1 and miR-211-5p was analyzed to illuminate their roles in osteogenic differentiation and osteolysis. METHODS: The expression of PRNCR1, miR-211-5p and C-X-C chemokine receptor-4 (CXCR4) protein in tissues and mesenchymal stem cells (MSCs) were determined by qRT-PCR and Western blot, separately. The osteogenic differentiation was assessed by ALP activity detection and ARS staining...
May 15, 2018: Gene
https://www.readbyqxmd.com/read/29774077/long-noncoding-rna-mir503hg-a-prognostic-indicator-inhibits-tumor-metastasis-by-regulating-the-hnrnpa2b1-nf-%C3%AE%C2%BAb-pathway-in-hepatocellular-carcinoma
#4
Hui Wang, Linhui Liang, Qiongzhu Dong, Lin Huan, Jia He, Botai Li, Chen Yang, Haojie Jin, Lin Wei, Chengtao Yu, Fangyu Zhao, Jinjun Li, Ming Yao, Wenxin Qin, Lunxiu Qin, Xianghuo He
Long noncoding RNAs (lncRNAs) have been associated with hepatocellular carcinoma (HCC), but the underlying molecular mechanisms of their specific association with hepatocarcinogenesis have not been fully explored. Methods: miR503HG was identified by microarray and validated by real-time PCR. Survival analysis was evaluated using the Kaplan-Meier method and assessed using the log-rank test. In vitro and in vivo assays were preformed to explore the biological effects of miR503HG in HCC cells. The interaction of miR503HG with HNRNPA2B1 was identified by RNA pull-down and RNA immunoprecipitation...
2018: Theranostics
https://www.readbyqxmd.com/read/29773901/ets-1-promoter-associated-noncoding-rna-regulates-the-nono-erg-ets-1-axis-to-drive-gastric-cancer-progression
#5
Dan Li, Yajun Chen, Hong Mei, Wanju Jiao, Huajie Song, Lin Ye, Erhu Fang, Xiaojing Wang, Feng Yang, Kai Huang, Liduan Zheng, Qiangsong Tong
Emerging studies have indicated the essential functions of long noncoding RNAs (lncRNAs) during cancer progression. However, whether lncRNAs contribute to the upregulation of v-ets erythroblastosis virus E26 oncogene homolog 1 (Ets-1), an established oncogenic protein facilitating tumor invasion and metastasis, in gastric cancer remains elusive. Herein, we identified Ets-1 promoter-associated noncoding RNA (pancEts-1) as a novel lncRNA associated with the gastric cancer progression via mining of publicly available datasets and rapid amplification of cDNA ends...
May 18, 2018: Oncogene
https://www.readbyqxmd.com/read/29772439/long-noncoding-rna-meg3-inhibits-proliferation-of-chronic-myeloid-leukemia-cells-by-sponging-microrna21
#6
Ziye Li, Lin Yang, Xiaojun Liu, Ziyuan Nie, Jianmin Luo
The long noncoding RNA (lnc) maternally expressed 3 (MEG3) is downregulated in many types of cancers. However, the relationship between lncRNA MEG3, microRNA-21 (miR-21) and chronic myeloid leukemia (CML) blast crisis is unknown. This study examined bone marrow samples from 40 CML patients and 10 healthy donors. Proliferation and apoptosis assays, real-time polymerase chain reaction (PCR), bisulfite sequencing PCR, Western blotting, luciferase assay, RNA pull-down, RNA immunoprecipitation (RIP), co-immunoprecipitation (CoIP) and Chromatin immunoprecipitation (ChIP) were performed...
May 14, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29769336/cytoplasmic-re-localization-and-colocalization-with-viroplasms-of-host-cell-proteins-and-their-role-in-rotavirus-infection
#7
Poonam Dhillon, Varsha N Tandra, Sandip G Chorghade, Nima D Namsa, Lipika Sahoo, C Durga Rao
Rotavirus replicates in the cytoplasm of infected cells in unique virus-induced cytoplasmic inclusion bodies called viroplasms (VM), which are nucleated by two essential viral non-structural proteins NSP2 and NSP5. However, the precise composition of the VM, intracellular localization of host proteins during virus infection, their association with VM or role in rotavirus growth remained largely unexplored. Mass-spectrometry analyses revealed the presence of several host heterogeneous nuclear ribonucleoproteins (hnRNPs) and AU-rich element-binding proteins (ARE-BPs) and cytoplasmic proteins from uninfected MA104 cell extracts in the pull-down (PD) complexes of purified viroplasmic proteins NSP2 and NSP5...
May 16, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29767363/identification-of-transcriptional-regulators-that-bind-to-long-noncoding-rnas-by-rna-pull-down-and-rna-immunoprecipitation
#8
Xiangbo Ruan, Ping Li, Haiming Cao
Long noncoding RNAs (lncRNAs) are RNA transcripts that are at least 200 nucleotides long and lack coding potential, and they have been demonstrated to be involved in a wide range of biological processes. Many lncRNAs, especially those enriched in nucleus, have been found to regulate gene expression at transcriptional level. Regulation of gene transcription by lncRNAs are mainly mediated by transcriptional regulators (TRs) which interact with lncRNAs. LncRNAs can either enhance or suppress TR's activity, which depends on different mechanism and cellular context...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29760716/metabolomic-fingerprints-of-individual-algal-cells-using-the-single-probe-mass-spectrometry-technique
#9
Mei Sun, Zhibo Yang, Boris Wawrik
Traditional approaches for the assessment of physiological responses of microbes in the environment rely on bulk filtration techniques that obscure differences among populations as well as among individual cells. Here, were report on the development on a novel micro-scale sampling device, referred to as the "Single-probe," which allows direct extraction of metabolites from living, individual phytoplankton cells for mass spectrometry (MS) analysis. The Single-probe is composed of dual-bore quartz tubing which is pulled using a laser pipette puller and fused to a silica capillary and a nano-ESI...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29760557/uca1-promotes-cell-proliferation-and-invasion-and-inhibits-apoptosis-through-regulation-of-the-mir129-sox4-pathway-in-renal-cell-carcinoma
#10
Qian Liu, Yang Li, Weiling Lv, Guangwei Zhang, Xin Tian, Xiaodong Li, Hepeng Cheng, Chaoyang Zhu
Background: Renal cell carcinoma (RCC) is the most common cancer in kidney malignancies. UCA1 has been identified as an oncogenic lncRNA in multiple cancers, including RCC. However, the underlying molecular mechanism of UCA1 involved in RCC progression is far from being addressed. Methods: Reverse-transcription quantitative polymerase chain reaction (RT-qPCR) assays were used to measure expressions of UCA1, miR129, and SOX4 mRNA. Western blot assays were employed to detect SOX4 protein expression...
2018: OncoTargets and Therapy
https://www.readbyqxmd.com/read/29757292/desthiobiotin-streptavidin-affinity-mediated-purification-of-rna-interacting-proteins-in-mesothelioma-cells
#11
Jelena Kresoja-Rakic, Emanuela Felley-Bosco
The in vitro RNA-pulldown is still largely used in the first steps of protocols aimed at identifying RNA-binding proteins that recognize specific RNA structures and motifs. In this RNA-pulldown protocol, commercially synthesized RNA probes are labeled with a modified form of biotin, desthiobiotin, at the 3' terminus of the RNA strand, which reversibly binds to streptavidin and thus allows elution of proteins under more physiological conditions. The RNA-desthiobiotin is immobilized through interaction with streptavidin on magnetic beads, which are used to pull down proteins that specifically interact with the RNA of interest...
April 25, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29752342/lncrna-prncr1-regulates-osteogenic-differentiation-in-osteolysis-after-hip-replacement-by-targeting-mir-211-5p
#12
Zong-Ming Gong, Zhen-Yu Tang, Xiao-Liang Sun
Background Osteogenic differentiation and osteolysis after hip replacement are both associated with bone metabolism. Interaction between the long non-coding RNA (lncRNA) prostate cancer non-coding RNA 1 (PRNCR1) and miR-211-5p was analyzed to illuminate their roles in osteogenic differentiation and osteolysis. Methods The expression of PRNCR1, miR-211-5p and C-X-C chemokine receptor-4 (CXCR4) protein in tissues and mesenchymal stem cells (MSCs) were determined by qRT-PCR and western blot, separately. The osteogenic differentiation was assessed with Alkaline phosphatase (ALP) activity detection and ARS staining...
May 11, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/29751054/%C3%AE-elemene-enhances-gap-43-expression-and-neurite-outgrowth-by-inhibiting-rhoa-kinase-activation-in-rats-with-spinal-cord-injury
#13
Jingyu Wang, Heyangzi Li, Ying Yao, Yucheng Ren, Jiangtao Lin, Jue Hu, Mingzhi Zheng, Xinghui Song, Tengfei Zhao, Ying-Ying Chen, Yueliang Shen, Yong-Jian Zhu, Lin-Lin Wang
RhoA signalling pathway inhibitors such as Y27632 (a ROCK inhibitor) have recently been applied as treatments for spinal cord injury (SCI) because they promote neurite outgrowth and axonal regeneration in neurons. β-elemene, a compound that is extracted from a natural plant (Curcuma zedoary), influences the expression level of RhoA protein. Whether it can promote neurite outgrowth in motor neurons or enhance locomotor recovery in SCI remains unclear. Here, we initially demonstrated that β-elemene promotes neurite outgrowth of ventral spinal cord 4...
May 8, 2018: Neuroscience
https://www.readbyqxmd.com/read/29742155/the-hsv-1-mechanisms-of-cell-to-cell-spread-and-fusion-are-critically-dependent-on-host-ptp1b
#14
Jillian C Carmichael, Hiroki Yokota, Rebecca C Craven, Anthony Schmitt, John W Wills
All herpesviruses have mechanisms for passing through cell junctions, which exclude neutralizing antibodies and offer a clear path to neighboring, uninfected cells. In the case of herpes simplex virus type 1 (HSV-1), direct cell-to-cell transmission takes place between epithelial cells and sensory neurons, where latency is established. The spreading mechanism is poorly understood, but mutations in four different HSV-1 genes can dysregulate it, causing neighboring cells to fuse to produce syncytia. Because the host proteins involved are largely unknown (other than the virus entry receptor), we were intrigued by an earlier discovery that cells infected with wild-type HSV-1 will form syncytia when treated with salubrinal...
May 9, 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29739365/histone-methyltransferase-setdb1-promotes-cells-proliferation-and-migration-by-interacting-withtiam1-in-hepatocellular-carcinoma
#15
Yuqin Zhang, Jing Huang, Qisheng Li, Keli Chen, Yonghao Liang, Zetao Zhan, Feng Ye, Wen Ni, Longhua Chen, Yi Ding
BACKGROUND: SETDB1 is a histone H3K9 methyltransferase, which plays a significant role in the occurrence and progression of tumors. Previous studies have confirmed that T-lymphom invasion and metastasis gene (Tiam1) is a protein associated with the metastasis of hepatocellular carcinoma (HCC); however, we have not yet been successful in elucidating the specific mechanism of HCC. METHODS: Yeast two-hybrid test was conducted to screen proteins that interacted with Tiam1 gene...
May 8, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29738871/identification-of-litopenaeus-vannamei-bip-as-a-novel-cellular-attachment-protein-for-white-spot-syndrome-virus-by-using-a-biotinylation-based-affinity-chromatography-method
#16
Zengzhi Yuan, Meng Chen, Jingting Wang, Zhuoyu Li, Xuyun Geng, Jinsheng Sun
White spot syndrome virus (WSSV) is a dangerous threat to shrimp farming that also attacks a wide range of crustaceans. Knowledge of the surface protein-protein interactions between the pathogen and host is very crucial to unraveling the molecular pathogenesis mechanisms of WSSV. In this study, LvBiP (Litopenaeus vannamei immunoglobulin heavy-chain-binding protein) was identified as a novel WSSV binding protein of L. vannamei by a biotinylation based affinity chromatography method. By using pull-down and ELISA assays, the binding of recombinant LvBiP to WSSV was proved to be specific and ATP- dependent...
May 5, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29730556/new-peptide-my1340-revert-the-inhibition-effect-of-vegf-on-dendritic-cells-differentiation-and-maturation-via-blocking-vegf-nrp-1-axis-and-inhibit-tumor-growth-in-vivo
#17
Zheng Mo, Fei Yu, Su Han, Songhua Yang, Liangliang Wu, Peng Li, Shunchang Jiao
The development and clinical application of immunostimulatory therapy provides us a new and exciting strategy in cancer treatment of which the agents act on crucial receptors. Given the fact that Neuropilin-1(NRP-1) is essential for vascular endothelial growth factor (VEGF) to inhibit LPS-dependent maturation of dendritic cells (DCs), it may present a potentially meaningful target in cancer immunotherapy. To explore this hypothesis, we synthesized a novel polypeptide called MY1340 consist of 32 amino acids with the aim of targeting VEGF-NRP-1 axis...
May 3, 2018: International Immunopharmacology
https://www.readbyqxmd.com/read/29729190/baicalein-targets-gtpase-mediated-autophagy-to-eliminate-liver-tumor-initiating-stem-cell-like-cells-resistant-to-mtorc1-inhibition
#18
Raymond Wu, Ramachandran Murali, Yasuaki Kabe, Samuel W French, Yi-Ming Chiang, Siyu Liu, Linda Sher, Clay C Wang, Stan Louie, Hidekazu Tsukamoto
Drug resistance is a major problem in the treatment of liver cancer. mTORC1 inhibitors have been tested for the treatment of liver cancer based on hyperactive mTOR in this malignancy. However, their clinical trials showed poor outcome due likely to their ability to upregulate CD133 and promote chemoresistance. CD133+ tumor initiating stem cell-like cells (TICs) isolated from mouse and human liver tumors are chemoresistant, and identification of an approach to abrogate this resistance is desired. In search for a compound which rescinds TIC's resistance to mTORC1 inhibition and improves chemotherapy, we identified baicalein (BC) which selectively chemosensitizes TICs and the human HCC cell line Huh7 cells but not mouse and human primary hepatocytes...
May 5, 2018: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/29729126/the-tog-protein-stu2-xmap215-interacts-covalently-and-non-covalently-with-sumo
#19
Matt Greenlee, Annabel Alonso, Maliha Rahman, Nida Meednu, Kayla Davis, Victoria Tabb, River Cook, Rita K Miller
Stu2p is the yeast member of the XMAP215/Dis1/ch-TOG family of microtubule-associated proteins that promote microtubule polymerization. However, the factors that regulate its activity are not clearly understood. Here we report that Stu2p in the budding yeast Saccharomyces cerevisiae interacts with SUMO by covalent and non-covalent mechanisms. Stu2p interacted by two-hybrid analysis with the yeast SUMO Smt3p, its E2 Ubc9p, and the E3 Nfi1p. A region of Stu2p containing the dimerization domain was both necessary and sufficient for interaction with SUMO and Ubc9p...
May 5, 2018: Cytoskeleton
https://www.readbyqxmd.com/read/29726904/rna-binding-protein-trinucleotide-repeat-containing-6a-regulates-the-formation-of-circular-rna-0006916-with-important-functions-in-lung-cancer-cells
#20
Xin Dai, Nan Zhang, Ying Cheng, Ti Yang, Yingnan Chen, Zhenzhong Liu, Zhishan Wang, Chengfeng Yang, Yiguo Jiang
Circular RNAs (circRNAs) are widespread and diverse endogenous RNAs distinct from traditional linear RNAs, which may regulate gene expression in eukaryotes. However, the function of human circRNAs, including their potential role in lung cancer, remains largely unknown. We screened the circRNA circ0006916, which was evidently down-regulated in 16HBE-T cells (anti-benzopyrene-trans-7, 8-dihydrodiol-9, 10-epoxide-transformed human bronchial epithelial cells), and in A549 and H460 cell lines. Silencing of circ0006916, but not its parental gene homer scaffolding protein 1 (HOMER1), promoted cell proliferation via speeding up the cell cycle process rather than by inhibiting apoptosis; conversely, overexpression of circ0006916 had the opposite effect...
May 3, 2018: Carcinogenesis
keyword
keyword
20373
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"