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https://www.readbyqxmd.com/read/29166741/-the-study-of-expression-and-prognostic-value-of-cd123-in-acute-myeloid-leukemia-bone-marrow-blasts
#1
W Q Yue, G S Tang, M Liu, H Cheng, J Ding, T Wang, J M Wang, X X Hu, W P Zhang, L Chen, J M Yang
Objective: To study the expression of CD123 in bone marrow (BM) blasts of acute myeloid leukemia (AML) patients to explore the relationship between CD123 expression and therapeutic response and prognosis. Methods: This study retrospectively analyzed expression and distribution of CD123 in BM blasts in 137 cases of newly diagnosed AML (excluded M(3)) , CD123 detected by flow cytometry≥20% was defined as positive, including 84 CD123(+) AML and 53 CD123(-) AML, efficacy and prognosis were compared between the two groups...
October 14, 2017: Zhonghua Xue Ye Xue za Zhi, Zhonghua Xueyexue Zazhi
https://www.readbyqxmd.com/read/29166740/-factors-associated-with-early-treatment-response-in-adults-with-acute-myeloid-leukemia
#2
X Ren, T Zhao, J Wang, H H Zhu, H Jiang, J S Jia, S M Yang, B Jiang, D B Wang, X J Huang, Q Jiang
Objective: To explore the factors influencing early treatment responses in adult with de novo acute myeloid leukemia (AML) . Methods: Data of consecutive newly-diagnosed AML (non-acute promyelocytic leukemia) adults were analyzed retrospectively. To assess the impact of clinical characteristics at diagnosis and induction regimen on achieving morphologic leukemia-free state (MLFS) , blood counts and minimal residual leukemia (MRD, positive MRD defined as RQ-PCR WT1 mRNA ≥0.6% and/or any level of abnormal blast population detected by flow cytometry) at the time of achieving MLFS...
October 14, 2017: Zhonghua Xue Ye Xue za Zhi, Zhonghua Xueyexue Zazhi
https://www.readbyqxmd.com/read/29166739/-study-of-the-effects-and-mechanism-of-all-trans-retinoic-acid-on-leukemic-cell-line-u937-cells-with-npm1-mutation
#3
S W Qiu, Y L Wan, Y J Jia, Q Rao, M Wang, J X Wang
Objective: To investigate the effect and mechanism of all-trans retinoic acid (ATRA) on leukemic cell line U937 cells with NPM1 mutation. Methods: Human acute myeloid leukemia cell line U937 was explored, NPM1 mutated (A type) plasmids were transfected into U937 to form stable clones A1 and A2, which were identified by Western blot and Co-immunoprecipitation. The cell proliferation was measured by methylthiazolyl tetrazolium bromide (MTT) ; cell cycle and cell apoptosis were explored by flow cytometric; cell colony formation was measured by microscope count, the molecular pathways related to cell proliferation were measured by Western blot...
October 14, 2017: Zhonghua Xue Ye Xue za Zhi, Zhonghua Xueyexue Zazhi
https://www.readbyqxmd.com/read/29166713/-advances-in-the-research-of-molecular-mechanism-of-negative-pressure-wound-therapy-in-improving-wound-healing
#4
Y Liu, D H Hu
Recently, negative pressure wound therapy (NPWT) is a rising technology to improve wound healing. In clinical application, it benefits fast debridement and wound close, limits infection, and promotes wound healing. It is an effective therapy for all kinds of acute or chronic wound. Currently, researches demonstrate that NPWT promotes angiogenesis, granulation tissue growth, and extracellular matrix remodeling through regulating the signaling of anti-inflammatory cytokines, mechanicalreceptor and chemoreceptor, which is related to several growth factors and inflammatory factors...
November 20, 2017: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/29166661/ultraconserved-elements-uces-resolve-the-phylogeny-of-australasian-smurf-weevils
#5
Matthew H Van Dam, Athena W Lam, Katayo Sagata, Bradley Gewa, Raymond Laufa, Michael Balke, Brant C Faircloth, Alexander Riedel
Weevils (Curculionoidea) comprise one of the most diverse groups of organisms on earth. There is hardly a vascular plant or plant part without its own species of weevil feeding on it and weevil species diversity is greater than the number of fishes, birds, reptiles, amphibians and mammals combined. Here, we employ ultraconserved elements (UCEs) designed for beetles and a novel partitioning strategy of loci to help resolve phylogenetic relationships within the radiation of Australasian smurf-weevils (Eupholini)...
2017: PloS One
https://www.readbyqxmd.com/read/29166625/ambient-surface-mass-spectrometry-ion-mobility-spectrometry-of-intact-proteins
#6
REVIEW
Rian L Griffiths, Klaudia I Kocurek, Helen J Cooper
Ambient surface mass spectrometry encompasses a broad range of sampling and ionization techniques. To date, only a small subset of these, based on liquid microjunction extraction, have proven suitable for intact protein analysis from thin tissue sections. Liquid extraction surface analysis shows particular promise for this application. Recently, a range of ion mobility spectrometry approaches have been coupled with ambient mass spectrometry. Improvements in signal-to-noise ratios, decreased chemical noise and separation of molecular classes have been described for the analysis of various biological substrates...
November 18, 2017: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/29166615/olfactory-experience-and-developmental-stage-dependent-control-of-cpeb4-regulates-c-fos-mrna-translation-for-granule-cell-survival
#7
Ching-San Tseng, Hsu-Wen Chao, Hsien-Sung Huang, Yi-Shuian Huang
Mammalian olfactory bulbs (OBs) require continuous replenishment of interneurons (mainly granule cells [GCs]) to support local circuits throughout life. Two spatiotemporally distinct waves of postnatal neurogenesis contribute to expanding and maintaining the GC pool. Although neonate-born GCs have a higher survival rate than adult-born GCs, the molecular mechanism underlying this survival remains unclear. Here, we find that cytoplasmic polyadenylation element-binding protein 4 (CPEB4) acts as a survival factor exclusively for early postnatal GCs...
November 21, 2017: Cell Reports
https://www.readbyqxmd.com/read/29166614/critical-modulation-of-hematopoietic-lineage-fate-by-hepatic-leukemia-factor
#8
Martin Wahlestedt, Vasileios Ladopoulos, Isabel Hidalgo, Manuel Sanchez Castillo, Rebecca Hannah, Petter Säwén, Haixia Wan, Monika Dudenhöffer-Pfeifer, Mattias Magnusson, Gudmundur L Norddahl, Berthold Göttgens, David Bryder
A gradual restriction in lineage potential of multipotent stem/progenitor cells is a hallmark of adult hematopoiesis, but the underlying molecular events governing these processes remain incompletely understood. Here, we identified robust expression of the leukemia-associated transcription factor hepatic leukemia factor (Hlf) in normal multipotent hematopoietic progenitors, which was rapidly downregulated upon differentiation. Interference with its normal downregulation revealed Hlf as a strong negative regulator of lymphoid development, while remaining compatible with myeloid fates...
November 21, 2017: Cell Reports
https://www.readbyqxmd.com/read/29166612/impediment-of-replication-forks-by-long-non-coding-rna-provokes-chromosomal-rearrangements-by-error-prone-restart
#9
Takaaki Watanabe, Michael Marotta, Ryusuke Suzuki, Scott J Diede, Stephen J Tapscott, Atsushi Niida, Xiongfong Chen, Lila Mouakkad, Anna Kondratova, Armando E Giuliano, Sandra Orsulic, Hisashi Tanaka
Naturally stalled replication forks are considered to cause structurally abnormal chromosomes in tumor cells. However, underlying mechanisms remain speculative, as capturing naturally stalled forks has been a challenge. Here, we captured naturally stalled forks in tumor cells and delineated molecular processes underlying the structural evolution of circular mini-chromosomes (double-minute chromosomes; DMs). Replication forks stalled on the DM by the co-directional collision with the transcription machinery for long non-coding RNA...
November 21, 2017: Cell Reports
https://www.readbyqxmd.com/read/29166608/s-nitrosylation-of-pink1-attenuates-pink1-parkin-dependent-mitophagy-in-hipsc-based-parkinson-s-disease-models
#10
Chang-Ki Oh, Abdullah Sultan, Joseph Platzer, Nima Dolatabadi, Frank Soldner, Daniel B McClatchy, Jolene K Diedrich, John R Yates, Rajesh Ambasudhan, Tomohiro Nakamura, Rudolf Jaenisch, Stuart A Lipton
Mutations in PARK6 (PINK1) and PARK2 (Parkin) are linked to rare familial cases of Parkinson's disease (PD). Mutations in these genes result in pathological dysregulation of mitophagy, contributing to neurodegeneration. Here, we report that environmental factors causing a specific posttranslational modification on PINK1 can mimic these genetic mutations. We describe a molecular mechanism for impairment of mitophagy via formation of S-nitrosylated PINK1 (SNO-PINK1). Mitochondrial insults simulating age- or environmental-related stress lead to increased SNO-PINK1, inhibiting its kinase activity...
November 21, 2017: Cell Reports
https://www.readbyqxmd.com/read/29166573/interaction-of-hsp90-with-phospholipid-model-membranes
#11
Muhan Zhang, Daoying Wang, Pengpeng Li, Chong Sun, Rong Xu, Zhiming Geng, Weimin Xu, Zhaoqi Dai
Heat shock protein 90 (Hsp90) is an essential molecular chaperone with versatile functions in cell homeostatic control under both normal and stress conditions. Hsp90 has been found to be expressed on the cell surface, but the mechanism of Hsp90 association to the membrane remains obscure. In this study, the direct interaction of Hsp90 and phospholipid vesicles was characterized, and the role of Hsp90 on membrane physical state was explored. Using surface plasmon resonance (SPR), we observed a strong interaction between Hsp90 and different compositions of lipid...
November 19, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29166560/analytical-challenges-and-recent-advances-in-mass-spectrometry-based-lipidomics
#12
Yepy Hardi Rustam, Gavin Edmund Reid
Although no internationally accepted consensus definition exists, lipids can be broadly defined as "fatty acids and their derivatives, and substances related biosynthetically or functionally to these compounds". Lipids play critical roles as (i) the major structural components of biological membranes, and as functional and regulatory components of membrane protein signaling, (ii) bioactive intra- and inter-cellular signaling molecules, and (iii) energy storage molecules for the maintenance of energy homeostasis...
November 22, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29166558/understanding-the-cellular-uptake-of-ph-responsive-zwitterionic-gold-nanoparticles-a-computer-simulation-study
#13
Xuebo Quan, Daohui Zhao, Libo Li, Jian Zhou
Surface functionalization of nanoparticles (NPs) with stealth polymers (e.g., hydrophilic and zwitterionic polymers) has become a common strategy to resist non-specific protein adsorption recently. Understanding the role of surface decoration on NP-biomembrane interactions is of great significance to promote the application of NPs in biomedical fields. Herein, using coarse-grained molecular dynamics (CGMD) simulations, we investigate the interactions between stealth polymer-coated gold nanoparticles (AuNPs) and lipid membranes...
November 22, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29166557/impact-of-crosslinker-valency-on-gold-nanoparticle-aggregate-formation-and-cellular-uptake
#14
Alice Liu, Jacob M Berlin
Synthesis of spherical, biocompatible nanoparticle aggregates using a small molecular crosslinker is a simple and flexible approach for the controlled assembly of gold nanoparticles. This strategy can be extended to a variety of crosslinkers, making it possible to the test the effect of crosslinker properties on aggregate formation and physicochemical properties. Here we synthesized aggregates using a series of structurally homologous crosslinkers with differing valencies. These aggregates have the same size, morphology, surface charge, surface coating, and stability in salt, media, and low pH conditions, but they differ in their stability to cyanide etching and uptake by cells...
November 22, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29166542/identification-of-cytochrome-p450-cyp-isoforms-involved-in-the-metabolism-of-artocarpin-and-assessment-of-its-drug-drug-interaction-ddi
#15
Wei Qu, Xuezheng Liu
Artocarpin isolated from an agricultural plant Artocarpus communis has shows anti-inflammation and anticancer activities. In this study, we utilized recombinant human UDP-glucuronosyltransferasesupersomes (UGTs) and human liver microsomes (HLMs) to explore its inhibitory effect on UGTs and cytochrome p450 enzymes(CYPs). Chemical inhibition studies and screening assays with recombinant human CYPs were used to identify if CYP isoform is involved in artocarpin metabolism. Artocarpin showed strong inhibition against UGT1A3, UGT1A6, UGT1A7, UGT1A8, UGT1A9, UGT1A10, UGT2B7, CYP2C8 and CYP3A4...
November 22, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/29166538/antimicrobial-photodynamic-therapy-associated-with-conventional-endodontic-treatment-a-clinical-and-molecular-microbiological-study
#16
Caroline C da Silva, Sérgio P Chaves Júnior, Gabriela L D Pereira, Karla B F C da Fontes, Lívia A A Antunes, Helvécio C C Póvoa, Leonardo S Antunes, Natalia L P Póvoa Iorio
This study evaluated antimicrobial photodynamic therapy (aPDT) as an adjunct to endodontic treatment. Ten uniradicular teeth (control group (CG)= 4 (2 and test group (TG)= 6) with primary endodontic infections, from both genders, between 17 and 65 years old, were analyzed. Microbiological samples were collected before and after chemical-mechanical instrumentation (CMI), after aPDT (for the TG), and after the removal of the temporary restorations (second session). In TG, the aPDT was performed with 100 μg/mL methylene blue and irradiated with low power laser (InGaAIP, 660 nm; 100 mW; 40 sec) with a fiber-coupled optical laser...
November 22, 2017: Photochemistry and Photobiology
https://www.readbyqxmd.com/read/29166494/sporotrichosis-an-update-on-epidemiology-etiopathogenesis-laboratory-and-clinical-therapeutics
#17
Rosane Orofino-Costa, Priscila Marques de Macedo, Anderson Messias Rodrigues, Andréa Reis Bernardes-Engemann
In the late 90's there was a change in both the route of transmission and the people at risk for sporotrichosis. This zoonotic cat-man alternative transmission route elicited changes in strategies to control the epidemic. There was a progressive increase in the number of cases involving especially children and the elderly. In addition to becoming hyperendemic, uncommon clinical pictures like immunoreactive clinical presentations or severe systemic cases have emerged. New species were identified and classified through molecular tools using more virulent clinical isolates, like S...
September 2017: Anais Brasileiros de Dermatologia
https://www.readbyqxmd.com/read/29166427/myxobolus-marajoensis-sp-n-myxosporea-myxobolidae-parasite-of-the-freshwater-catfish-rhamdia-quelen-from-the-brazilian-amazon-region
#18
Jacqueline Abrunhosa, José Ledamir Sindeaux-Neto, Ândrea Kely Dos Santos, Igor Hamoy, Edilson Matos
This study provides morphological and molecular data of a new parasite species found in the muscle layer of the intestinal tract of the South American silver catfish, Rhamdia quelen from Marajó Island region (Pará State, Brazil), an important fishery resource with recognized potential for fish farming. The morphology of these parasites was reanalyzed and phylogenetic analyses were run on their 18S rDNA gene sequences. The spores were morphologically distinct from those of other Myxobolus species described previously...
November 17, 2017: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
https://www.readbyqxmd.com/read/29166413/comprehensive-molecular-profiling-of-718-multiple-myelomas-reveals-significant-differences-in-mutation-frequencies-between-african-and-european-descent-cases
#19
Zarko Manojlovic, Austin Christofferson, Winnie S Liang, Jessica Aldrich, Megan Washington, Shukmei Wong, Daniel Rohrer, Scott Jewell, Rick A Kittles, Mary Derome, Daniel Auclair, David Wesley Craig, Jonathan Keats, John D Carpten
Multiple Myeloma (MM) is a plasma cell malignancy with significantly greater incidence and mortality rates among African Americans (AA) compared to Caucasians (CA). The overall goal of this study is to elucidate differences in molecular alterations in MM as a function of self-reported race and genetic ancestry. Our study utilized somatic whole exome, RNA-sequencing, and correlated clinical data from 718 MM patients from the Multiple Myeloma Research Foundation CoMMpass study Interim Analysis 9. Somatic mutational analyses based upon self-reported race corrected for ancestry revealed significant differences in mutation frequency between groups...
November 2017: PLoS Genetics
https://www.readbyqxmd.com/read/29166412/mir-195-inhibits-macrophages-pro-inflammatory-profile-and-impacts-the-crosstalk-with-smooth-muscle-cells
#20
Joao Paulo Bras, Andreia Machado Silva, George A Calin, Mario Adolfo Barbosa, Susana Gomes Santos, Maria Ines Almeida
Macrophages are a main component of atherosclerotic plaques. Recent studies suggest that pro-inflammatory M1 macrophages are pro-atherogenic while M2 macrophages promote plaque stability. Moreover, toll-like receptor signalling pathways are implicated in atherosclerotic plaque formation, evolution and regression. We propose microRNAs as key regulators of these processes. In this context, our goal is to promote inflammation resolution using miR-195 to reduce M1-like macrophage polarization and to evaluate the molecular mechanisms underlying such effect, as well as to explore the functional consequences for smooth muscle cell recruitment...
2017: PloS One
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