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https://www.readbyqxmd.com/read/29330810/correlation-of-glioma-proliferation-and-hypoxia-by-luciferase-magnetic-resonance-and-positron-emission-tomography-imaging
#1
Michael Karsy, David L Gillespie, Kevin P Horn, Lance D Burrell, Jeffery T Yap, Randy L Jensen
Gliomas are the most common type of primary, malignant brain tumor and significantly impact patients, who have a median survival of ~1 year depending on mutational background. Novel imaging modalities such as luciferase bioluminescence, micro-magnetic resonance imaging (micro-MRI), micro-computerized tomography (micro-CT), and micro-positron emission tomography (micro-PET) have expanded the portfolio of tools available to study this disease. Hypoxia, a key oncogenic driver of glioma and mechanism of resistance, can be studied in vivo by the concomitant use of noninvasive MRI and PET imaging...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29327783/nonsurgical-management-of-human-immunodeficiency-virus-associated-parotid-cysts-a-systematic-review-and-meta-analysis
#2
Akash N Naik, William B Clinkscales, Masanari G Kato, Shaun A Nguyen, M Boyd Gillespie
BACKGROUND: The purpose of this clinical review was to analyze the effectiveness of nonsurgical management options for human immunodeficiency virus (HIV)-associated parotid cysts. METHODS: We conducted systematic and meta-analysis reviews. Primary outcomes were complete or partial responses. RESULTS: Systematic review identified 12 relevant studies. The average rates of complete response for antiretroviral therapy (ART), sclerotherapy, and fine-needle aspiration (FNA) were 52...
January 12, 2018: Head & Neck
https://www.readbyqxmd.com/read/29322466/using-rt-qpcr-for-quantifying-mycobacteria-marinum-from-in%C3%A2-vitro-and-in-vivo-samples
#3
Han Xaio, Stephen H Gillespie
Mycobacterium marinum, the causative agent of fish tuberculosis, is rarely a human pathogen causing a chronic skin infection. It is now wildely used as a model system in animal models, especially in zebra fish model, to study the pathology of tuberculosis and as a means of screening new anti-tuberculosis agent. To facilitate such research, quantifying the viable count of M. marinum bacteria is a crucial step. The main approach used currently is still by counting the number of colony forming units (cfu), a method that has been in place for almost 100 years...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29322461/enhanced-methodologies-for-detecting-phenotypic-resistance-in-mycobacteria
#4
Robert J H Hammond, Vincent O Baron, Sam Lipworth, Stephen H Gillespie
Lipid droplets found in algae and other microscopic organisms have become of interest to many researchers partially because they carry the capacity to produce bio-oil for the mass market. They are of importance in biology and clinical practice because their presence can be a phenotypic marker of an altered metabolism, including reversible resistance to antibiotics, prompting intense research.A useful stain for detecting lipid bodies in the lab is Nile red. It is a dye that exhibits solvatochromism; its absorption band varies in spectral position, shape and intensity with the nature of its solvent environment, it will fluoresce intensely red in polar environment and blue shift with the changing polarity of its solvent...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29322457/detecting-phenotypically-resistant-mycobacterium-tuberculosis-using-wavelength-modulated-raman-spectroscopy
#5
Vincent O Baron, Mingzhou Chen, Simon O Clark, Ann Williams, Kishan Dholakia, Stephen H Gillespie
Raman spectroscopy is a non-destructive and label-free technique. Wavelength modulated Raman (WMR) spectroscopy was applied to investigate Mycobacterium tuberculosis cell state, lipid rich (LR) and lipid poor (LP). Compared to LP cells, LR cells can be up to 40 times more resistant to key antibiotic regimens. Using this methodology single lipid rich (LR) from lipid poor (LP) bacteria can be differentiated with both high sensitivity and specificity. It can also be used to investigate experimentally infected frozen tissue sections where both cell types can be differentiated...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29319593/effects-of-interruptions-on-triage-process-in-emergency-department-a-prospective-observational-study
#6
Kimberly D Johnson, Gordon L Gillespie, Kimberly Vance
For 130 million people seeking emergency treatment in the United States, incomplete or inaccurate triage examination can result in delays, which could compromise patient outcomes. The purpose of this study was to identify triage interruptions and determine how interruptions affect the triage process. A significant difference was seen in triage duration between interrupted and uninterrupted interviews. Understanding the impact of interruptions on patient outcomes will allow nurses and other health care providers to develop interventions to mitigate the impact...
January 9, 2018: Journal of Nursing Care Quality
https://www.readbyqxmd.com/read/29315497/health-care-disparities-among-octogenarians-and-nonagenarians-with-stage-iii-lung-cancer
#7
Richard J Cassidy, Xinyan Zhang, Jeffrey M Switchenko, Pretesh R Patel, Joseph W Shelton, Sibo Tian, Ronica H Nanda, Conor E Steuer, Rathi N Pillai, Taofeek K Owonikoko, Suresh S Ramalingam, Felix G Fernandez, Seth D Force, Theresa W Gillespie, Walter J Curran, Kristin A Higgins
BACKGROUND: To the authors' knowledge, the practice patterns for patients aged more than 80 years with stage III non-small cell lung cancer (NSCLC) is not well known. The purpose of the current study was to investigate factors predictive of and the impact on overall survival (OS) after concurrent chemoradiation (CRT) among patients aged ≥80 years with American Joint Committee on Cancer stage III NSCLC in the National Cancer Data Base (NCDB). METHODS: In the NCDB, patients aged ≥80 years who were diagnosed with stage III NSCLC from 2004 to 2013 with complete treatment records were identified...
January 8, 2018: Cancer
https://www.readbyqxmd.com/read/29305766/a-novel-lips-assay-for-insulin-autoantibodies
#8
Daniela Liberati, Rebecca C Wyatt, Cristina Brigatti, Ilaria Marzinotto, Maurizio Ferrari, Elena Bazzigaluppi, Emanuele Bosi, Ben T Gillard, Kathleen M Gillespie, Frans Gorus, Ilse Weets, Eric Balti, Lorenzo Piemonti, Peter Achenbach, Alistair J K Williams, Vito Lampasona
AIMS: Insulin autoantibodies (IAA) are often the first marker of autoimmunity detected in children in the preclinical phase of type 1 diabetes (T1D). Currently, the vast majority of laboratories adopt the radiobinding micro-assay (RBA) for measuring IAA. Our aim was to replace RBA with a novel non-radioactive IAA Luciferase Immuno Precipitation System (LIPS) assay with improved performance. METHODS: We developed (pro)insulin antigens with alternative placements of a NanoLuc™ luciferase reporter (NLuc)...
January 5, 2018: Acta Diabetologica
https://www.readbyqxmd.com/read/29304124/the-effect-of-dna-degradation-bias-in-passive-sampling-devices-on-metabarcoding-studies-of-arthropod-communities-and-their-associated-microbiota
#9
Henrik Krehenwinkel, Marisa Fong, Susan Kennedy, Edward Greg Huang, Suzuki Noriyuki, Luis Cayetano, Rosemary Gillespie
PCR amplification bias is a well-known problem in metagenomic analysis of arthropod communities. In contrast, variation of DNA degradation rates is a largely neglected source of bias. Differential degradation of DNA molecules could cause underrepresentation of taxa in a community sequencing sample. Arthropods are often collected by passive sampling devices, like malaise traps. Specimens in such a trap are exposed to varying periods of suboptimal storage and possibly different rates of DNA degradation. Degradation bias could thus be a significant issue, skewing diversity estimates...
2018: PloS One
https://www.readbyqxmd.com/read/29302612/an-application-of-extended-normalisation-process-theory-in-a-randomised-controlled-trial-of-a-complex-social-intervention-process-evaluation-of-the-strengthening-families-programme-10-14-in-wales-uk
#10
Jeremy Segrott, Simon Murphy, Heather Rothwell, Jonathan Scourfield, David Foxcroft, David Gillespie, Jo Holliday, Kerenza Hood, Claire Hurlow, Sarah Morgan-Trimmer, Ceri Phillips, Hayley Reed, Zoe Roberts, Laurence Moore
Purpose: Process evaluations generate important data on the extent to which interventions are delivered as intended. However, the tendency to focus only on assessment of pre-specified structural aspects of fidelity has been criticised for paying insufficient attention to implementation processes and how intervention-context interactions influence programme delivery. This paper reports findings from a process evaluation nested within a randomised controlled trial of the Strengthening Families Programme 10-14 (SFP 10-14) in Wales, UK...
December 2017: SSM—Population Health
https://www.readbyqxmd.com/read/29301685/the-role-of-temperature-in-egg-development-of-three-aquatic-insects-lestagella-penicillata-ephemeroptera-aphanicercella-scutata-plecoptera-chimarra-ambulans-trichoptera-from-south-africa
#11
Vere Ross-Gillespie, Mike D Picker, Helen F Dallas, Jenny A Day
No abstract text is available yet for this article.
January 2018: Journal of Thermal Biology
https://www.readbyqxmd.com/read/29301519/iterative-co-creation-for-improved-hand-hygiene-and-aseptic-techniques-in-the-operating-room-experiences-from-the-safe-hands-study
#12
Annette Erichsen Andersson, Maria Frödin, Lisen Dellenborg, Lars Wallin, Jesper Hök, Brigid M Gillespie, Ewa Wikström
BACKGROUND: Hand hygiene and aseptic techniques are essential preventives in combating hospital-acquired infections. However, implementation of these strategies in the operating room remains suboptimal. There is a paucity of intervention studies providing detailed information on effective methods for change. This study aimed to evaluate the process of implementing a theory-driven knowledge translation program for improved use of hand hygiene and aseptic techniques in the operating room...
January 4, 2018: BMC Health Services Research
https://www.readbyqxmd.com/read/29299394/using-herbarium-derived-dnas-to-assemble-a-large-scale-dna-barcode-library-for-the-vascular-plants-of-canada
#13
Maria L Kuzmina, Thomas W A Braukmann, Aron J Fazekas, Sean W Graham, Stephanie L Dewaard, Anuar Rodrigues, Bruce A Bennett, Timothy A Dickinson, Jeffery M Saarela, Paul M Catling, Steven G Newmaster, Diana M Percy, Erin Fenneman, Aurélien Lauron-Moreau, Bruce Ford, Lynn Gillespie, Ragupathy Subramanyam, Jeannette Whitton, Linda Jennings, Deborah Metsger, Connor P Warne, Allison Brown, Elizabeth Sears, Jeremy R Dewaard, Evgeny V Zakharov, Paul D N Hebert
Premise of the study: Constructing complete, accurate plant DNA barcode reference libraries can be logistically challenging for large-scale floras. Here we demonstrate the promise and challenges of using herbarium collections for building a DNA barcode reference library for the vascular plant flora of Canada. Methods: Our study examined 20,816 specimens representing 5076 of 5190 vascular plant species in Canada (98%). For 98% of the specimens, at least one of the DNA barcode regions was recovered from the plastid loci rbcL and matK and from the nuclear ITS2 region...
December 2017: Applications in Plant Sciences
https://www.readbyqxmd.com/read/29299360/prognostic-relevance-of-human-papillomavirus-infection-in-anal-squamous-cell-carcinoma-analysis-of-the-national-cancer-data-base
#14
Jaymin Jhaveri, Lael Rayfield, Yuan Liu, Mudit Chowdhary, Richard J Cassidy, Nicholas A Madden, Daniel G Tanenbaum, Theresa W Gillespie, Pretesh R Patel, Kirtesh R Patel, Jerome C Landry
Background: To examine the prognostic relevance of human papillomavirus (HPV) infection for anal squamous cell carcinoma (ASCC) patients treated with chemoradiation (CRT) in the National Cancer Data Base (NCDB). Methods: The 2014 NCDB was queried for non-metastatic, histologically confirmed, ASCC patients diagnosed between 2004 and 2013. Patients were required to have HPV status documented in order to be eligible. Patients were then stratified into two groups: HPV+ and HPV-...
December 2017: Journal of Gastrointestinal Oncology
https://www.readbyqxmd.com/read/29297015/what-exactly-is-an-snf-ist
#15
Suzanne M Gillespie, Cari R Levy, Paul R Katz
No abstract text is available yet for this article.
January 1, 2018: JAMA Internal Medicine
https://www.readbyqxmd.com/read/29286686/chemical-continuous-time-random-walks
#16
Tomás Aquino, Marco Dentz
Kinetic Monte Carlo methods such as the Gillespie algorithm model chemical reactions as random walks in particle number space. The interreaction times are exponentially distributed under the assumption that the system is well mixed. We introduce an arbitrary interreaction time distribution, which may account for the impact of incomplete mixing on chemical reactions, and in general stochastic reaction delay, which may represent the impact of extrinsic noise. This process defines an inhomogeneous continuous time random walk in particle number space, from which we derive a generalized chemical master equation...
December 8, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/29280599/surgical-counting-a-perioperative-nurse-s-perspective
#17
Vicky Warwick, Brigid Gillespie
The Australian College of Perioperative Nurses (ACORN) have developed a set of standards to guide clinical practice within the perioperative setting. The ACORN standard 'Management of accountable items used during surgery and procedures' (ACORN 2016) details a process for perioperative nurses to follow when undertaking a surgical count.
May 2017: Australian Nursing & Midwifery Journal
https://www.readbyqxmd.com/read/29279094/entamoeba-histolytica-infection-in-wild-lemurs-associated-with-proximity-to-humans
#18
Leo J Ragazzo, Sarah Zohdy, Mamitiana Velonabison, James Herrera, Patricia C Wright, Thomas R Gillespie
Amoebiasis, caused by Entamoeba histolytica, affects 50 million people worldwide, and results in 100,000 deaths annually. It is particularly prevalent in developing nations where poverty and poor sanitation contribute to contamination of food and water. E. histolytica is also a zoonotic protozoan parasite with the potential to infect non-human primates. Lemurs, primates endemic to Madagascar, are the most threatened mammalian group in the world due to habitat loss. As forests disappear, humans and lemurs come into more frequent contact, and the potential for E...
January 15, 2018: Veterinary Parasitology
https://www.readbyqxmd.com/read/29261166/ancient-dna-resolves-the-history-of-tetragnatha-araneae-tetragnathidae-spiders-on-rapa-nui
#19
Darko D Cotoras, Gemma G R Murray, Joshua Kapp, Rosemary G Gillespie, Charles Griswold, W Brian Simison, Richard E Green, Beth Shapiro
Rapa Nui is one of the most remote islands in the world. As a young island, its biota is a consequence of both natural dispersals over the last ~1 million years and recent human introductions. It therefore provides an opportunity to study a unique community assemblage. Here, we extract DNA from museum-preserved and newly field-collected spiders from the genus Tetragnatha to explore their history on Rapa Nui. Using an optimized protocol to recover ancient DNA from museum-preserved spiders, we sequence and assemble partial mitochondrial genomes from nine Tetragnatha species, two of which were found on Rapa Nui, and estimate the evolutionary relationships between these and other Tetragnatha species...
December 20, 2017: Genes
https://www.readbyqxmd.com/read/29247210/estimating-and-mitigating-amplification-bias-in-qualitative-and-quantitative-arthropod-metabarcoding
#20
Henrik Krehenwinkel, Madeline Wolf, Jun Ying Lim, Andrew J Rominger, Warren B Simison, Rosemary G Gillespie
Amplicon based metabarcoding promises rapid and cost-efficient analyses of species composition. However, it is disputed whether abundance estimates can be derived from metabarcoding due to taxon specific PCR amplification biases. PCR-free approaches have been suggested to mitigate this problem, but come with considerable increases in workload and cost. Here, we analyze multilocus datasets of diverse arthropod communities, to evaluate whether amplification bias can be countered by (1) targeting loci with highly degenerate primers or conserved priming sites, (2) increasing PCR template concentration, (3) reducing PCR cycle number or (4) avoiding locus specific amplification by directly sequencing genomic DNA...
December 15, 2017: Scientific Reports
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