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Micropropagation orchid

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https://www.readbyqxmd.com/read/27866249/investigation-of-endophytic-bacterial-community-in-supposedly-axenic-cultures-of-pineapple-and-orchids-with-evidence-on-abundant-intracellular-bacteria
#1
Natalia Pimentel Esposito-Polesi, Monita Fiori de Abreu-Tarazi, Cristina Vieira de Almeida, Siu Mui Tsai, Marcílio de Almeida
Asepsis, defined as the absence of microbial contamination, is one of the most important requirements of plant micropropagation. In long-term micropropagated cultures, there may occasionally occur scattered microorganism growth in the culture medium. These microorganisms are common plant components and are known as latent endophytes. Thus, the aim of this research was to investigate the presence of endophytic bacteria in asymptomatic pineapple and orchid microplants, which were cultivated in three laboratories for 1 year...
November 19, 2016: Current Microbiology
https://www.readbyqxmd.com/read/27108306/protocols-for-in-vitro-mass-multiplication-and-analysis-of-medicinally-important-phenolics-of-a-salep-orchid-satyrium-nepalense-d-don-salam-mishri
#2
Shashi B Babbar, Deepak K Singh
Satyrium nepalense is a rare and threatened medicinal orchid, populations of which in its native habitats are dwindling because of indiscriminate collections and habitat destruction, thus necessitating the development of methods for its in situ and ex situ conservation. Because of non-endospermous nature of the seeds and the immature embryos at seed dispersal stage, orchids cannot be seed-propagated as other plants. Micropropagation, using plant tissue culture techniques, offers an effective method for the multiplication of orchids...
2016: Methods in Molecular Biology
https://www.readbyqxmd.com/read/26046143/dendrobium-micropropagation-a-review
#3
REVIEW
Jaime A Teixeira da Silva, Jean Carlos Cardoso, Judit Dobránszki, Songjun Zeng
Dendrobium is one of the largest and most important (ornamentally and medicinally) orchid genera. Tissue culture is now an established method for the effective propagation of members of this genus. This review provides a detailed overview of the Dendrobium micropropagation literature. Through a chronological analysis, aspects such as explant, basal medium, plant growth regulators, culture conditions and final organogenic outcome are chronicled in detail. This review will allow Dendrobium specialists to use the information that has been documented to establish, more efficiently, protocols for their own germplasm and to improve in vitro culture conditions based on the optimized parameters detailed in this review...
May 2015: Plant Cell Reports
https://www.readbyqxmd.com/read/25693491/dendrobium-micropropagation-a-review
#4
Jaime A Teixeira da Silva, Jean Carlos Cardoso, Judit Dobránszki, Songjun Zeng
Dendrobium is one of the largest and most important (ornamentally and medicinally) orchid genera. Tissue culture is now an established method for the effective propagation of members of this genus. This review provides a detailed overview of the Dendrobium micropropagation literature. Through a chronological analysis, aspects such as explant, basal medium, plant growth regulators, culture conditions and final organogenic outcome are chronicled in detail. This review will allow Dendrobium specialists to use the information that has been documented to establish, more efficiently, protocols for their own germplasm and to improve in vitro culture conditions based on the optimized parameters detailed in this review...
February 19, 2015: Plant Cell Reports
https://www.readbyqxmd.com/read/25606433/genetic-stability-and-phytochemical-analysis-of-the-in-vitro-regenerated-plants-of-dendrobium-nobile-lindl-an-endangered-medicinal-orchid
#5
Paromik Bhattacharyya, Suman Kumaria, Reemavareen Diengdoh, Pramod Tandon
An efficient genetically stable regeneration protocol with increased phytochemical production has been established for Dendrobium nobile, a highly prized orchid for its economic and medicinal importance. Protocorm like bodies (PLBs) were induced from the pseudostem segments using thidiazuron (TDZ; 1.5 mg/l), by-passing the conventional auxin-cytokinin complement approach for plant regeneration. Although, PLB induction was observed at higher concentrations of TDZ, plantlet regeneration from those PLBs was affected adversely...
December 2014: Meta Gene
https://www.readbyqxmd.com/read/24136821/orchid-protocorm-like-bodies-are-somatic-embryos
#6
Yung-I Lee, Shan-Te Hsu, Edward C Yeung
PREMISE OF THE STUDY: Protocorm-like bodies (PLBs) of orchids are important in orchid micropropagation and outwardly resemble somatic embryos in form and development. To determine whether PLBs are truly embryogenetic, we compared PLBs with somatic embryos and zygotic embryos to determine whether they had similar surface molecules and whether hydroxyproline-rich glycoprotein (HRGP) inhibitors similarly alter their growth. METHODS: Embryogenic calluses (ECs), zygotic embryos, and protocorms were collected for histological and histochemical studies with light microscopy...
November 2013: American Journal of Botany
https://www.readbyqxmd.com/read/21390606/clonal-propagation-of-orchids
#7
D Jones, B Tisserat
This chapter will deal with methods of clonal propagation for members of the two major morphological groups of orchids. The first group, sympodials, includes such genera as Cymbidium, Cattleya, Dendrobium, and Oncidium. They are characterized by a multi-branchingrhizome that can supply an abundance of axillary shoots for use as explants. They were among the first orchids to be successfully propagated, and techniques for their in vitro initiation (i.e., establishment) and subsequent proliferation are well established (1-8)...
1990: Methods in Molecular Biology
https://www.readbyqxmd.com/read/21207276/micropropagation-of-phalaenopsis-orchids-via-protocorms-and-protocorm-like-bodies
#8
Kee Yoeup Paek, Eun Joo Hahn, So Young Park
Phalaenopsis orchids have high economic value in the floriculture industry. Hybridization or cross-pollination in the breeding program have proven to be very reliable techniques for the production of a wide range of successful cultivars with attractive combinations of spray length, bud number, flower color and type, fragrance, seasonality, and compactness. In vitro propagation makes it possible to clonally mass propagate hybrids of commercial value and conserved species. However, in vitro culture technologies are still a challenge because of the slow growth of plantlets, low multiplication rate, poor rooting, and somaclonal variation...
2011: Methods in Molecular Biology
https://www.readbyqxmd.com/read/20190397/analysis-of-genetic-stability-through-intersimple-sequence-repeats-molecular-markers-in-micropropagated-plantlets-of-anoectochilus-formosanus-hayata-a-medicinal-plant
#9
Fusheng Zhang, Yali Lv, Hailing Dong, Shunxing Guo
Anoectochilus formosanus HAYATA, commonly known as "Jewel Orchids," which has been used as Chinese folk medicines, is being subjected to a huge crisis, whose wild resources have gradually become more and more scarce. Hence, micropropagation protocol by axillary branching established for A. formosanus was employed for large-scale commercial production. In this study, due to the somaclonal variation and on the basis of some virtue of intersimple sequence repeats (ISSR) analysis, we firstly utilized the ISSR primers to investigate the genetic stability of A...
2010: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/18786626/the-role-of-activated-charcoal-in-plant-tissue-culture
#10
REVIEW
T Dennis Thomas
Activated charcoal has a very fine network of pores with large inner surface area on which many substances can be adsorbed. Activated charcoal is often used in tissue culture to improve cell growth and development. It plays a critical role in micropropagation, orchid seed germination, somatic embryogenesis, anther culture, synthetic seed production, protoplast culture, rooting, stem elongation, bulb formation etc. The promotary effects of AC on morphogenesis may be mainly due to its irreversible adsorption of inhibitory compounds in the culture medium and substancially decreasing the toxic metabolites, phenolic exudation and brown exudate accumulation...
November 2008: Biotechnology Advances
https://www.readbyqxmd.com/read/16117854/transcription-analysis-of-peloric-mutants-of-phalaenopsis-orchids-derived-from-tissue-culture
#11
Ya Huei Chen, Yi Jung Tsai, Jian Zhi Huang, Fure Chyi Chen
Tissue culture has been widely used for mass propagation of Phalaenopsis. However, somaclonal variation occurred during micropropagation process posed a severe problem by affecting product quality. In this study, wild type and peloric flower buds of Phalaenopsis hybrids derived from flower stalk nodal culture were used for cDNA-RAPD and cDNA suppression subtractive hybridization analyses in order to study their genetic difference in terms of expressed sequence tags. A total of 209 ESTs from normal flower buds and 230 from mutants were sequenced...
August 2005: Cell Research
https://www.readbyqxmd.com/read/15341672/systemic-endopolyploidy-in-spathoglottis-plicata-orchidaceae-development
#12
Maocheng Yang, Chiang Shiong Loh
BACKGROUND: Endopolyploidy is developmentally regulated. Presence of endopolyploidy as a result of endoreduplication has been characterized in insects, mammals and plants. The family Orchidaceae is the largest among the flowering plants. Many of the members of the orchid family are commercially micropropagated. Very little has been done to characterize the ploidy variation in different tissues of the orchid plants during development. RESULTS: The DNA contents and ploidy level of nuclei extracted from various tissues of a tropical terrestrial orchid Spathoglottis plicata were examined by flow cytometry...
September 1, 2004: BMC Cell Biology
https://www.readbyqxmd.com/read/11218831/micropropagation-of-terrestrial-orchids-anoectochilus-sikkimensis-and-anoectochilus-regalis
#13
A Gangaprasad, P G Latha, S Seeni
Two horticulturally important jewel orchids of the genus Anoectochilus were successfully micropropagated. Isolated nodes of A. sikkimensis collected from Sikkim in Eastern Himalayas and subsequently reared under nursery conditions and A. regalis collected from Western Ghats in Southern India were cultured for 12 weeks on Woody Plant Medium (WPM) to produce a maximum of 4.8 and 5.6 callus--free axillary shoots respectively at 95 and 98% efficiency. During reculture of the explants from in vitro raised shoots under the same conditions, the total number of shoots obtained from the nodes (21...
February 2000: Indian Journal of Experimental Biology
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