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Filipe Cristóvão, Carla Andrea Alonso, Gilberto Igrejas, Margarida Sousa, Vanessa Silva, José Eduardo Pereira, Carmen Lozano, Gerardo Cortés-Cortés, Carmen Torres, Patrícia Poeta
The clonal diversity of extended-spectrum-β-lactamase (ESBL)-producing Escherichia coli isolates from nine different species of wild animals from distinct regions of Portugal and Spain and their content in replicon plasmids were analyzed. Among the initial 53 ESBL-producing E. coli isolates that were studied (from previous studies), 28 were selected, corresponding to different animal origins with distinct ESBL types and pulsed-field gel electrophoresis (PFGE) patterns. These 28 isolates produced different ESBLs ascribed to the following families: CTX-M, SHV and TEM...
March 1, 2017: FEMS Microbiology Letters
Ilias Apostolakos, Eelco Franz, Angela H A M van Hoek, Alice Florijn, Christiaan Veenman, Marianne M Sloet-van Oldruitenborgh-Oosterbaan, Cindy Dierikx, Engeline van Duijkeren
Objectives: To investigate the occurrence and characteristics of ESBL/AmpC-producing Escherichia coli in faecal samples from horses at one equine clinic in the Netherlands. Methods: A total of 91 horses, including residents and patients, were sampled. ESBL/AmpC-producing E. coli were identified by a combination disc diffusion test. Phylogenetic groups and MLST were determined. ESBL/AmpC genes were analysed using PCR and sequencing. Plasmids were characterized by transformation and PCR-based replicon typing...
March 13, 2017: Journal of Antimicrobial Chemotherapy
Brandon Eilertson, Liang Chen, Kalyan D Chavda, Barry N Kreiswirth
Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae have now become a global public health threat. However, the origin of this pandemic and the characterization of pre-2003 blaKPC-harboring plasmids remain unknown. Here we used next-generation sequencing to characterize two KPC-2-producing K. pneumoniae and Kmichiganensis isolates collected from a New York City hospital in 1997. Although identified in two different Klebsiella species, the blaKPC-2 gene was harbored by Tn4401b transposons on two highly similar IncN plasmids...
April 2017: Antimicrobial Agents and Chemotherapy
Yuki Ohno, Akihiro Nakamura, Eriko Hashimoto, Hiroko Matsutani, Noriyuki Abe, Saori Fukuda, Kohno Hisashi, Masaru Komatsu, Fumihiko Nakamura
Recently, carbapenemase-producing Enterobacteriaceae (CPE) have been spreading worldwide and have become a threat in healthcare systems. We investigated the isolation frequency and molecular epidemiological characteristics of CPE isolated from clinical samples collected at a primary care hospital over the four years of 2010-2013 in Japan. CPE were detected in 17 (0.34%) of 4875 isolates by the broth microdilution method, sodium mercaptoacetate inhibition test, and modified Hodge test using meropenem disks. The frequency of CPE isolates was 0...
February 1, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
S Datta, S Mitra, P Chattopadhyay, T Som, S Mukherjee, S Basu
To investigate the mobilizable elements associated with bla NDM-1 in Enterobacteriaceae isolated from septicaemic neonates at a NICU in India, during December, 2008-2011. An attempt was also made to understand whether there was a pattern in the temporal acquisition of bla NDM-1 within the unit. Transferability of carbapenem resistance was tested by conjugation and transformation. Plasmid types and addiction systems were analysed. The genetic background of bla NDM-1 and association with class 1 integron were evaluated by PCR mapping...
February 2017: European Journal of Clinical Microbiology & Infectious Diseases
Jingna An, Ling Guo, Lin Zhou, Yanning Ma, Yanping Luo, Chuanmin Tao, Jiyong Yang
New Delhi metallo-β-lactamase (NDM)-producing Enterobacteriaceae (NPE) shows prevalence in China. Little is known about the mechanisms related to the spread of NPE. Recently, a total of 51 non-duplicated NPE isolates were collected from a tertiary-care hospital in China and analysed for genetic relatedness by PFGE, antimicrobial susceptibility by Etest and sequence type by multilocus sequence typing. S1-PFGE and Southern blot analysis or PCR amplification were used for plasmid profiling. Between 2014 and 2015, 22 Escherichia coli, 10 Klebsiella pneumoniae, 9 Enterobacter cloacae, 2 Enterobacter aerogenes, 3 Providencia rettgeri, 1 Klebsiella oxytoca, 1 Proteus mirabilis, 1 Citrobacter freundii, 1 Citrobacterwerkmanii and 1 Raoultella planticola were identified as NPE...
November 2016: Journal of Medical Microbiology
Wei Feng, Dongsheng Zhou, Qian Wang, Wenbo Luo, Defu Zhang, Qiang Sun, Yigang Tong, Weijun Chen, Fengjun Sun, Peiyuan Xia
A total of 26 blaIMP-4-carrying strains of Pseudomonas aeruginosa and Klebsiella pneumoniae were isolated from 2009 to 2013 in a Chinese teaching hospital, and these strains can be assigned into multiple sequence types or allelic profiles as determined by multilocus sequence typing. Of these strains, P. aeruginosa P378 and K. pneumoniae 1220 harbor the IMP-4-encoding plasmids pP378-IMP and p1220-IMP, respectively, whose complete nucleotide sequences are determined to be genetically closely related to the IncN1-type plasmid pIMP-HZ1...
September 19, 2016: Scientific Reports
Vanesa García, Patricia García, Irene Rodríguez, Rosaura Rodicio, M Rosario Rodicio
pUO-SeVR1 is a resistance derivative of pSEV, the virulence plasmid specific of Salmonella enterica serovar Enteritidis. It was first detected in a Spanish isolate involved in gastroenteritis, but closely related plasmids are widespread in highly invasive isolates originating from Africa. According to its nucleotide sequence, pUO-SeVR1 consists of 110,982bp with a GC content of 53.2%. It confers resistance to multiple antimicrobial agents and contains a wealth of mobile genetic elements, including 14 copies of IS26 which have played a major role in the evolution of the plasmid...
September 9, 2016: Infection, Genetics and Evolution
Ya Wang, Wai-U Lo, Raymond Wai-Man Lai, Cindy Wing-Sze Tse, Rodney Allan Lee, Wei-Kwang Luk, Vincent Chi-Chung Cheng, Tak-Lun Que, Kin-Hung Chow, Pak-Leung Ho
OBJECTIVES: To characterize blaIMP-4-carrying plasmids originating from inpatients in Hong Kong. METHODS: Sixteen blaIMP-4-carrying plasmids identified among Enterobacteriaceae (nine Escherichia coli, four Klebsiella pneumoniae, two Citrobacter freundii and one Enterobacter cloacae) recovered from 15 patients were characterized. The isolates, collected during January 2010 to December 2013, were retrospectively investigated by plasmid sequencing, molecular and fitness studies...
January 2017: Journal of Antimicrobial Chemotherapy
Nicole Stoesser, Anna E Sheppard, Gisele Peirano, Robert Sebra, Tarah Lynch, Luke Anson, Andrew Kasarskis, Mary R Motyl, Derrick W Crook, Johann D Pitout
OXA-48-like enzymes have emerged as important extended-spectrum β-lactamases/carbapenemases in Escherichia coli sequence type 131 (ST131). We report the structures of the first fully sequenced blaOXA-163 plasmid and of two other blaOXA-48 plasmids in this lineage. blaOXA-163 was located on a 71-kb IncN plasmid with other resistance genes. blaOXA-48 was present on IncL/M plasmids, genetically similar to other blaOXA-48 plasmid sequences, and consistent with interspecies/interlineage spread. The presence of blaOXA-48-like genes on epidemic plasmids in ST131 is of concern...
November 2016: Antimicrobial Agents and Chemotherapy
Naveen Kumar Devanga Ragupathi, Dhiviya Prabaa Muthuirulandi Sethuvel, Baby Abirami Shankar, Elakkiya Munusamy, Shalini Anandan, Balaji Veeraraghavan
Enteric fever is a major cause of concern in developing countries across the globe. The primary choice of antibiotics remains fluoroquinolones, followed by cephalosporins. Resistance to third-generation cephalosporins is rarely reported in Salmonella enterica serovar Typhi. This study reports the whole genome sequence of an S. Typhi isolate resistant to cefixime [minimum inhibitory concentration (MIC)=512μg/mL] by microbroth dilution. Interestingly, the isolate was negative for the cephalosporin resistance gene blaCTX-M by PCR, which is a known mechanism for higher cephalosporin resistance...
December 2016: Journal of Global Antimicrobial Resistance
Birgit Wolters, Arum Widyasari-Mehta, Robert Kreuzig, Kornelia Smalla
Pig manures are frequently used as fertilizer or co-substrate in biogas plants (BGPs) and typically contain antibiotic residues (ARs), as well as bacteria carrying resistance genes (RGs) and mobile genetic elements (MGEs). A survey of manures from eight pig fattening and six pig breeding farms and digestates from eight BGPs in Lower Saxony, Germany was conducted to evaluate the link between antibiotic usage and ARs to RGs and MGEs present in organic fertilizers. In total, 11 different antibiotics belonging to six substance classes were applied in the farms investigated...
November 2016: Applied Microbiology and Biotechnology
Dongryeoul Bae, Ohgew Kweon, Ashraf A Khan
The objective of this study was to determine antimicrobial resistance and elucidate the resistance mechanism in nontyphoidal Salmonella enterica serovars isolated from food products imported into the United States from 2011 to 2013. Food products contaminated with antimicrobial-resistant nontyphoidal S. enterica were mainly imported from Taiwan, Indonesia, Vietnam, and China. PCR, DNA sequencing, and plasmid analyses were used to characterize antimicrobial resistance determinants. Twentythree of 110 S. enterica isolates were resistant to various antimicrobial classes, including β-lactam, aminoglycoside, phenicol, glycopeptide, sulfonamide, trimethoprim, and/or fluoroquinolone antimicrobial agents...
August 2016: Journal of Food Protection
Juan Wang, James F Gibbons, Kathleen McGrath, Li Bai, Fengqin Li, Finola C Leonard, Roger Stephan, Séamus Fanning
OBJECTIVES: To characterize ESBL-encoding Escherichia coli cultured from pigs and their plasmids carrying these genes following conjugation into recipient strains. METHODS: Six ESBL-producing E. coli were recovered from faecal samples taken from pigs along with a further isolate from the environment of a farrowing house on three pig farms in Ireland. These isolates were characterized by phylogenetic grouping, MLST and ESBL genotype analyses. Conjugation experiments were carried out in broth mating assays...
November 2016: Journal of Antimicrobial Chemotherapy
Carla Rodrigues, Jan Bavlovič, Elisabete Machado, José Amorim, Luísa Peixe, Ângela Novais
KPC-3-producing bacteria are endemic in many countries but only recently became apparent their wide distribution in different Portuguese hospitals. The aim of this study is to characterize genetic backgrounds associated with bla KPC-3 among Klebsiella pneumoniae isolates recently identified on non-hospitalized patients in Portugal. Twenty KPC-producing K. pneumoniae identified between October 2014 and November 2015 in three different community laboratories were characterized. Isolates were mainly from patients from long-term care facilities (n = 11) or nursing homes (n = 6), most of them (75%) previously hospitalized in different Portuguese hospitals...
2016: Frontiers in Microbiology
Jia Du, Bin Li, Jianming Cao, Qing Wu, Huale Chen, Yuanbo Hou, En Zhang, Tieli Zhou
The emergence and dissemination of NDM-1 (New Delhi metallo-β-lactamase-1)-producing Enterobacteriaceae have resulted in a worldwide public health risk. This study described a high incidence and endemic spread of NDM-1-producing extensively drug-resistant Escherichia coli in a teaching hospital in Zhejiang province, China. We recovered six nonduplicated NDM-1-producing E. coli isolates from May 2014 to August 2014 with positive modified Hodge test and EDTA synergistic test. These isolates were highly resistant to β-lactam antimicrobials, aminoglycosides, and quinolones...
April 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
Amanda K Siqueira, Geovana B Michael, Daniela F Domingos, Mirtis M G Ferraz, Márcio G Ribeiro, Stefan Schwarz, Domingos S Leite
Escherichia coli is one of the major pathogens causing urinary tract infections (UTIs) and pyometra in dogs. The aims of this study were to investigate canine E. coli isolates for the presence of class 1 and 2 integrons by PCR/sequencing and to characterize these isolates and their integron-carrying plasmids. Isolates were characterized by phylotyping, XbaI-macrorestriction analysis and plasmid transfer experiments. Plasmids were analyzed by S1 nuclease-PFGE, replicon typing, conjugation and restriction analysis...
October 15, 2016: Veterinary Microbiology
Daniela Jones-Dias, Vera Manageiro, Rafael Graça, Daniel A Sampaio, Teresa Albuquerque, Patrícia Themudo, Luís Vieira, Eugénia Ferreira, Lurdes Clemente, Manuela Caniça
Salmonella enterica and Escherichia coli can inhabit humans and animals from multiple origins. These bacteria are often associated with gastroenteritis in animals, being a frequent cause of resistant zoonotic infections. In fact, bacteria from animals can be transmitted to humans through the food chain and direct contact. In this study, we aimed to assess the antibiotic susceptibility of a collection of S. enterica and E. coli recovered from animals of different sources, performing a genomic comparison of the plasmid-mediated quinolone resistance (PMQR)-producing isolates detected...
2016: Frontiers in Microbiology
Jing Xia, Jian Sun, Ke Cheng, Liang Li, Liang-Xing Fang, Meng-Ting Zou, Xiao-Ping Liao, Ya-Hong Liu
A total of 963 non-duplicate Escherichia coli strains isolated from food-producing animals between 2002 and 2012 were screened for the presence of the 16S rRNA methyltransferase genes. Among the positive isolates, resistance determinants to extended spectrum β-lactamases, plasmid-mediated quinolone resistance genes as well as floR and fosA/A3/C2 were detected using PCR analysis. These isolates were further subjected to antimicrobial susceptibility testing, molecular typing, PCR-based plasmid replicon typing and plasmid analysis...
May 30, 2016: Veterinary Microbiology
Joana Mourão, Sara Marçal, Paula Ramos, Joana Campos, Jorge Machado, Luísa Peixe, Carla Novais, Patrícia Antunes
OBJECTIVES: Factors driving the expansion of particular MDR Salmonella serotypes/clones are not completely understood. We assessed if emergent MDR Salmonella serotypes/clones were more enriched in metal tolerance genes (e.g. to Cu/Ag) than other less frequent ones, as an additional feature to survive in environments contaminated with metals. METHODS: Metal (Cu pco/Ag,Cu sil/Hg mer/As ars/Te ter) tolerance genes screening (PCR/sequencing), MICs of CuSO4/AgNO3 (aerobiosis/anaerobiosis), genetic element characterization (S1/I-CeuI PFGE) and conjugation assays were performed in a well-characterized Salmonella collection (n = 275 isolates; 2000-14; 49 serotypes/clones)...
August 2016: Journal of Antimicrobial Chemotherapy
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