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https://www.readbyqxmd.com/read/29620958/sequence-analysis-of-inca-c-and-inci1-plasmids-isolated-from-multidrug-resistant-salmonella-newport-using-single-molecule-real-time-sequencing
#1
Guojie Cao, Marc Allard, Maria Hoffmann, Tim Muruvanda, Yan Luo, Justin Payne, Kevin Meng, Shaohua Zhao, Patrick McDermott, Eric Brown, Jianghong Meng
Multidrug-resistant (MDR) plasmids play an important role in disseminating antimicrobial resistance genes. To elucidate the antimicrobial resistance gene compositions in A/C incompatibility complex (IncA/C) plasmids carried by animal-derived MDR Salmonella Newport, and to investigate the spread mechanism of IncA/C plasmids, this study characterizes the complete nucleotide sequences of IncA/C plasmids by comparative analysis. Complete nucleotide sequencing of plasmids and chromosomes of six MDR Salmonella Newport strains was performed using PacBio RSII...
April 5, 2018: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/29616895/ceftriaxone-resistant-salmonella-typhi-carries-an-inci1-st31-plasmid-encoding-ctx-m-15
#2
Bilal Djeghout, Senjuti Saha, Mohammad Saiful Islam Sajib, Arif Mohammad Tanmoy, Maksuda Islam, Gemma L Kay, Gemma C Langridge, Hubert P Endtz, John Wain, Samir K Saha
PURPOSE: Ceftriaxone is the drug of choice for typhoid fever and the emergence of resistant Salmonella Typhi raises major concerns for treatment. There are an increasing number of sporadic reports of ceftriaxone-resistant S. Typhi and limiting the risk of treatment failure in the patient and outbreaks in the community must be prioritized. This study describes the use of whole genome sequencing to guide outbreak identification and case management. METHODOLOGY: An isolate of ceftriaxone-resistant S...
April 4, 2018: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29609544/detection-of-ctx-m-15-harboring-escherichia-coli-isolated-from-wild-birds-in-tunisia
#3
Houssem Ben Yahia, Rym Ben Sallem, Ghassan Tayh, Naouel Klibi, Insaf Ben Amor, Haythem Gharsa, Abdellatif Boudabbous, Karim Ben Slama
BACKGROUND: The spreading of antibiotic resistant bacteria is becoming nowadays an alarming threat to human and animal health. There is increasing evidence showing that wild birds could significantly contribute to the transmission and spreading of drug-resistant bacteria. However, data for antimicrobial resistance in wild birds remain scarce, especially throughout Africa. The aims of this investigation were to analyze the prevalence of ESBL-producing E. coli in faecal samples of wild birds in Tunisia and to characterize the recovered isolates...
April 2, 2018: BMC Microbiology
https://www.readbyqxmd.com/read/29605531/high-prevalence-of-ceftriaxone-resistance-among-invasive-salmonella-enterica-serotype-choleraesuis-isolates-in-thailand-the-emergence-and-increase-of-ctx-m-55-in-ciprofloxacin-resistant-s-choleraesuis-isolates
#4
Sirirat Luk-In, Tanittha Chatsuwan, Chaiwat Pulsrikarn, Aroon Bangtrakulnonth, Ubolrat Rirerm, Wanla Kulwichit
S. Choleraesuis is a highly invasive zoonotic pathogen that causes a serious systemic infection in humans. The emergence and increase of resistance to ceftriaxone and ciprofloxacin among S. Choleraesuis has become a serious therapeutic problem. The present study demonstrated high frequency of antimicrobial resistance in Salmonella Choleraesuis among 414 nontyphoidal Salmonella isolates from bacteremic patients in Thailand. High rates of ceftriaxone (58.3%) and ciprofloxacin (19.6%) resistances were observed in S...
March 23, 2018: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/29563908/occurrence-of-bla-ndm-variants-among-enterobacteriaceae-from-a-neonatal-intensive-care-unit-in-a-northern-india-hospital
#5
Nayeem Ahmad, Shamsi Khalid, Syed M Ali, Asad U Khan
Carbapenem-resistance among enterobacteriaceae has become a global health concern. The objective of this study was to understand NDM producing enterobacteriaceae and their genetic basis of resistance, spreading in neonatal intensive care unit. Carbapenem resistant NDM producing enterobacteriaceae isolates were recovered from rectal swab and blood sample of infants admitted in NICU. These were determined by using Carba-NP test. All isolates were identified using BD PhoenixTM-100 and MICs were determined by broth microdilution method...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29549276/impact-of-human-associated-escherichia-coli-clonal-groups-in-antarctic-pinnipeds-presence-of-st73-st95-st141-and-st131
#6
Azucena Mora, Francisco Javier García-Peña, María Pilar Alonso, Susana Pedraza-Diaz, Luis Miguel Ortega-Mora, Daniel Garcia-Parraga, Cecilia López, Susana Viso, Ghizlane Dahbi, Juan Marzoa, Martin J Sergeant, Vanesa García, Jorge Blanco
There is growing concern about the spreading of human microorganisms in relatively untouched ecosystems such as the Antarctic region. For this reason, three pinniped species (Leptonychotes weddellii, Mirounga leonina and Arctocephalus gazella) from the west coast of the Antartic Peninsula were analysed for the presence of Escherichia spp. with the recovery of 158 E. coli and three E. albertii isolates. From those, 23 harboured different eae variants (α1, β1, β2, ε1, θ1, κ, ο), including a bfpA-positive isolate (O49:H10-A-ST206, eae-k) classified as typical enteropathogenic E...
March 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29519532/high-diversity-of-genes-and-plasmids-encoding-resistance-to-third-generation-cephalosporins-and-quinolones-in-clinical-escherichia-coli-from-commercial-poultry-flocks-in-italy
#7
Giulia Niero, Valeria Bortolaia, Michele Vanni, Luigi Intorre, Luca Guardabassi, Alessandra Piccirillo
The aim was to investigate occurrence and diversity of plasmid-mediated resistance to third-generation cephalosporins (3GC) and quinolones in clinical Escherichia coli from 200 industrial poultry farms across Italy. E. coli was isolated from colibacillosis lesions in turkeys (n = 109), broilers (n = 98) and layers (n = 22) between 2008 and 2012. 3GC-resistant isolates were screened for extended-spectrum and AmpC β-lactamase (ESBL/AmpC), while all isolates were tested for plasmid-mediated quinolone resistance (PMQR) genes...
March 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/29519517/longitudinal-study-of-escherichia-coli-plasmid-resistance-to-extended-spectrum-cephalosporins-in-free-range-broilers
#8
Sandrine Baron, Laetitia Le Devendec, Fabrice Touzain, Eric Jouy, Pierrick Lucas, Claire de Boisséson, Emeline Larvor, Isabelle Kempf
Resistance to extended-spectrum cephalosporins (ESCs) is mostly borne by conjugative plasmids. The aim of the present study was to evaluate the characteristics and diversity of ESC resistance plasmids in Escherichia coli from different free-range broiler flocks in France, and their persistence in flocks during rearing. Two hatcheries were selected. Faecal samples from 11 flocks were collected from before their arrival on the broiler production farm up to their slaughter at the end of the rearing period. A selection of 25 E...
March 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/29506073/host-specific-differences-in-the-contribution-of-an-esbl-inci1-plasmid-to-intestinal-colonization-by-escherichia-coli-o104-h4
#9
M Giles, S A Cawthraw, M AbuOun, C M Thomas, D Munera, M K Waldor, R M La Ragione, J M Ritchie
Objectives: To assess stability and contribution of a large ESBL-encoding IncI1 plasmid to intestinal colonization by Escherichia coli O104:H4 in two different mammalian hosts. Methods: Specific-pathogen-free 3-4-day-old New Zealand White rabbits and conventionally reared 6-week-old weaned lambs were orally infected with WT E. coli O104:H4 or the ESBL-plasmid-cured derivative, and the recovery of bacteria in intestinal homogenates and faeces monitored over time...
February 28, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29505913/occurrence-of-bla-cmy-42-through-inci1-plasmid-within-multidrug-resistant-escherichia-coli-from-a-tertiary-referral-hospital-of-india
#10
Birson Ingti, Pranjit Saikia, Deepjyoti Paul, Anand Prakash Maurya, Debadatta Dhar Chanda, Atanu Chakravarty, Chandrayee Deshamukhya, Amitabha Bhattacharjee
OBJECTIVES: Plasmids of different replicon types are believed to be associated with the carriage and transmission of antibiotic resistance genes. Thus present study was undertaken to check the association of CIT with particular plasmid types and to identify the E. coli strains involve in the maintenance of this determinant within the plasmid. METHODS: Screening of AmpC β-lactamase phenotypically by Modified Three Dimensional Extract Method followed by Antimicrobial Susceptibility and MIC determination...
March 2, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29483121/characterization-of-the-complete-nucleotide-sequences-of-imp-4-encoding-plasmids-belonging-to-diverse-inc-families-recovered-from-enterobacteriaceae-of-wildlife-origin
#11
Monika Dolejska, Costas C Papagiannitsis, Matej Medvecky, Lenka Davidova-Gerzova, Adam Valcek
The complete nucleotide sequences of six IMP-4-encoding plasmids recovered from Enterobacteriaceae of wildlife origin were characterized. Sequencing data showed that plasmids of different incompatibility groups (IncM, IncI1, IncF, and nontypeable [including an IncX5_2 and two pPrY2001-like]) carried the bla IMP-4 -carrying integrons, In809 or In1460. Most of the plasmids carried mph (A) region, and chrA -like, aac(3)-IId and bla TEM-1b genes. Finally, plasmid analysis revealed the involvement of two different, IS 26 - and Tn 1696 -associated, mechanisms in the mobilization of IMP-4-encoding integrons...
February 26, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29421359/emergence-of-plasmid-mediated-colistin-resistance-in-cmy-2-producing-escherichia-coli-of-lineage-st2197-in-a-tunisian-poultry-farm
#12
Elaa Maamar, Carla Andrea Alonso, Zaineb Hamzaoui, Nouha Dakhli, Mohamed Salah Abbassi, Sana Ferjani, Mabrouka Saidani, Ilhem Boutiba-Ben Boubaker, Carmen Torres
Our study aimed to investigate colistin resistance and the mechanisms involved in a collection of 35 extended-spectrum beta-lactamase (ESBL) and 13 CMY-2-producing E. coli strains which were previously recovered from chicken gut microbiota in Tunisia, as well as to determine the genetic location of mcr genes. Forty-eight ESBL and CMY-2-producing E. coli strains were obtained from 137 fecal samples of healthy chickens during 2013. These strains were tested for colistin resistance by the broth microdilution method, and screened for mcr-1 and mcr-2 genes by PCR...
January 29, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29360838/characterization-of-plasmids-harboring-blactx-m-and-blacmy-genes-in-e-coli-from-french-broilers
#13
Fabrice Touzain, Laetitia Le Devendec, Claire de Boisséson, Sandrine Baron, Eric Jouy, Agnès Perrin-Guyomard, Yannick Blanchard, Isabelle Kempf
Resistance to extended-spectrum cephalosporins (ESC) is a global health issue. The aim of this study was to analyze and compare plasmids coding for resistance to ESC isolated from 16 avian commensal and 17 avian pathogenic Escherichia coli (APEC) strains obtained respectively at slaughterhouse or from diseased broilers in 2010-2012. Plasmid DNA was used to transform E. coli DH5alpha, and the resistances of the transformants were determined. The sequences of the ESC-resistance plasmids prepared from transformants were obtained by Illumina (33 plasmids) or PacBio (1 plasmid)...
2018: PloS One
https://www.readbyqxmd.com/read/29340602/emergence-of-blactx-m-55-associated-with-fosa-rmtb-and-mcr-gene-variants-in-escherichia-coli-from-various-animal-species-in-france
#14
Agnese Lupo, Estelle Saras, Jean-Yves Madec, Marisa Haenni
Objectives: In Asian countries, blaCTX-M-55 is the second most common ESBL-encoding gene. blaCTX-M-55 frequently co-localizes with fosA and rmtB genes on epidemic plasmids, which remain sporadic outside Asia. During 2010-13, we investigated CTX-M-55-producing Escherichia coli isolates and their co-resistance to fosfomycin, aminoglycosides, fluoroquinolones and colistin as part of a global survey of ESBLs in animals in France. Methods: blaCTX-M-55, fosA, rmtB and plasmidic quinolone and colistin resistance genes were characterized by PCR, sequencing and hybridization experiments...
April 1, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29305512/molecular-epidemiology-of-dairy-cattle-associated-escherichia-coli-carrying-bla-ctx-m-genes-in-washington-state
#15
Josephine A Afema, Sara Ahmed, Thomas E Besser, Lisa P Jones, William M Sischo, Margaret A Davis
An increase in the prevalence of commensal Escherichia coli carrying bla CTX-M genes among dairy cattle was observed between 2008 and 2012 in Washington State. To study the molecular epidemiology of this change, we selected 126 bla CTX-M -positive and 126 bla CTX-M -negative isolates for determinations of the multilocus sequence types (MLSTs) and antibiotic resistance phenotypes from E. coli obtained during a previous study. For 99 isolates, we also determined the bla CTX-M alleles using PCR and sequencing and identified the replicon types of bla CTX-M -carrying plasmids...
March 15, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29284053/esbl-producing-escherichia-coli-in-swedish-gulls-a-case-of-environmental-pollution-from-humans
#16
Clara Atterby, Stefan Börjesson, Sofia Ny, Josef D Järhult, Sara Byfors, Jonas Bonnedahl
ESBL-producing bacteria are present in wildlife and the environment might serve as a resistance reservoir. Wild gulls have been described as frequent carriers of ESBL-producing E. coli strains with genotypic characteristics similar to strains found in humans. Therefore, potential dissemination of antibiotic resistance genes and bacteria between the human population and wildlife need to be further investigated. Occurrence and characterization of ESBL-producing E. coli in Swedish wild gulls were assessed and compared to isolates from humans, livestock and surface water collected in the same country and similar time-period...
2017: PloS One
https://www.readbyqxmd.com/read/29277529/comparative-genomics-of-rmtb-carrying-inci1-st136-plasmids-in-avian-escherichia-coli-isolates-from-chickens-in-china
#17
Li Yuan, Jian-Hua Liu, Xiang-Dang Du, Zhi-Yong Zong, Min Chen, Gong-Zheng Hu, Yu-Shan Pan
Eight rmtB-carrying avian Escherichia coli strains from a farm in China were characterised in our previous study, but little is known about the backbones and entire multiresistance regions (MRRs) of these plasmids. Here, three rmtB-carrying IncI1 ST136 plasmids were analysed by whole-plasmid sequencing and were compared. These plasmids were composed of an 83 470-bp IncI1 backbone carrying genes responsible for plasmid replication, transfer, maintenance and stability functions, as well as a 17 330-bp MRR for pEC006 and pEC007, and a 34 626-bp MRR for pEC008...
April 2018: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29238335/treatment-with-cefotaxime-affects-expression-of-conjugation-associated-proteins-and-conjugation-transfer-frequency-of-an-inci1-plasmid-in-escherichia-coli
#18
Thea S B Møller, Gang Liu, Anders Boysen, Line E Thomsen, Freja L Lüthje, Sisse Mortensen, Jakob Møller-Jensen, John E Olsen
Horizontal gene transfer (HGT) is the major mechanism responsible for spread of antibiotic resistance. Antibiotic treatment has been suggested to promote HGT, either by directly affecting the conjugation process itself or by selecting for conjugations subsequent to DNA transfer. However, recent research suggests that the effect of antibiotic treatment on plasmid conjugation frequencies, and hence the spread of resistance plasmids, may have been overestimated. We addressed the question by quantifying transfer proteins and conjugation frequencies of a bla CTX-M-1 encoding IncI1 resistance plasmid in Escherichia coli MG1655 in the presence and absence of therapeutically relevant concentrations of cefotaxime (CTX)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29227186/diversity-of-ctx-m-extended-spectrum-%C3%AE-lactamases-in-escherichia-coli-isolates-from-retail-raw-ground-beef-first-report-of-ctx-m-24-and-ctx-m-32-in-algeria
#19
Nesrine Rebbah, Yamina Messai, Pierre Châtre, Marisa Haenni, Jean Yves Madec, Rabah Bakour
The aim of this study was to investigate the prevalence and molecular features of extended-spectrum cephalosporin resistance in Escherichia coli isolates contaminating ground beef at retail in Algeria. Of 371 ground beef samples, 27.5% were found to contain cefotaxime-resistant E. coli isolates distributed into A (24.5%), B1 (60.8%), and D (14.7%) phylogroups. A rate of 88.2% of isolates had a multidrug-resistance phenotype. All strains were producers of CTX-M type extended-spectrum β-lactamases (ESBLs): CTX-M-1, CTX-M-3, CTX-M-14, CTX-M-15, CTX-M-24, or CTX-M-32...
December 11, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29185858/antimicrobial-resistance-in-escherichia-coli-isolated-from-wild-animals-in-poland
#20
Dariusz Wasyl, Magdalena Zając, Anna Lalak, Magdalena Skarżyńska, Ilona Samcik, Renata Kwit, Artur Jabłoński, Łukasz Bocian, Grzegorz Woźniakowski, Andrzej Hoszowski, Krzysztof Szulowski
Antimicrobial resistance was tested in Escherichia coli isolated from feces (n = 660) of red deer, roe deer, fallow deer, European bison, and wild boar shot in regional forests in Poland during two winter hunting seasons. Indicator E. coli (n = 542) was resistant against 11 of 14 tested compounds, mostly sulfamethoxazole, streptomycin, ampicillin, trimethoprim, and tetracycline (1.3-6.6% range). No significant differences were observed between boar and ruminant isolates. Most of deer and bison isolates showed no resistance...
November 29, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
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