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https://www.readbyqxmd.com/read/29421359/emergence-of-plasmid-mediated-colistin-resistance-in-cmy-2-producing-escherichia-coli-of-lineage-st2197-in-a-tunisian-poultry-farm
#1
Elaa Maamar, Carla Andrea Alonso, Zaineb Hamzaoui, Nouha Dakhli, Mohamed Salah Abbassi, Sana Ferjani, Mabrouka Saidani, Ilhem Boutiba-Ben Boubaker, Carmen Torres
Our study aimed to investigate colistin resistance and the mechanisms involved in a collection of 35 extended-spectrum beta-lactamase (ESBL) and 13 CMY-2-producing E. coli strains which were previously recovered from chicken gut microbiota in Tunisia, as well as to determine the genetic location of mcr genes. Forty-eight ESBL and CMY-2-producing E. coli strains were obtained from 137 fecal samples of healthy chickens during 2013. These strains were tested for colistin resistance by the broth microdilution method, and screened for mcr-1 and mcr-2 genes by PCR...
January 29, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29360838/characterization-of-plasmids-harboring-blactx-m-and-blacmy-genes-in-e-coli-from-french-broilers
#2
Fabrice Touzain, Laetitia Le Devendec, Claire de Boisséson, Sandrine Baron, Eric Jouy, Agnès Perrin-Guyomard, Yannick Blanchard, Isabelle Kempf
Resistance to extended-spectrum cephalosporins (ESC) is a global health issue. The aim of this study was to analyze and compare plasmids coding for resistance to ESC isolated from 16 avian commensal and 17 avian pathogenic Escherichia coli (APEC) strains obtained respectively at slaughterhouse or from diseased broilers in 2010-2012. Plasmid DNA was used to transform E. coli DH5alpha, and the resistances of the transformants were determined. The sequences of the ESC-resistance plasmids prepared from transformants were obtained by Illumina (33 plasmids) or PacBio (1 plasmid)...
2018: PloS One
https://www.readbyqxmd.com/read/29340602/emergence-of-blactx-m-55-associated-with-fosa-rmtb-and-mcr-gene-variants-in-escherichia-coli-from-various-animal-species-in-france
#3
Agnese Lupo, Estelle Saras, Jean-Yves Madec, Marisa Haenni
Objectives: In Asian countries, blaCTX-M-55 is the second most common ESBL-encoding gene. blaCTX-M-55 frequently co-localizes with fosA and rmtB genes on epidemic plasmids, which remain sporadic outside Asia. During 2010-13, we investigated CTX-M-55-producing Escherichia coli isolates and their co-resistance to fosfomycin, aminoglycosides, fluoroquinolones and colistin as part of a global survey of ESBLs in animals in France. Methods: blaCTX-M-55, fosA, rmtB and plasmidic quinolone and colistin resistance genes were characterized by PCR, sequencing and hybridization experiments...
January 10, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29305512/molecular-epidemiology-of-dairy-cattle-associated-escherichia-coli-carrying-blactx-m-genes-in-washington-state
#4
Josephine A Afema, Sara Ahmed, Thomas E Besser, Lisa P Jones, William M Sischo, Margaret A Davis
An increase in prevalence of commensal Escherichia coli carrying blaCTX-M genes among dairy cattle was observed between 2008 and 2012 in Washington State. To study the molecular epidemiology of this change, we selected 126 blaCTX-M-positive and 126 blaCTX-M-negative isolates for multi-locus sequence typing (MLST) and antibiotic resistance phenotyping from E. coli obtained during a previous study. For 99 isolates we also determined the blaCTX-M alleles using PCR and sequencing and identified the replicon types of blaCTX-M carrying plasmids...
January 5, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29284053/esbl-producing-escherichia-coli-in-swedish-gulls-a-case-of-environmental-pollution-from-humans
#5
Clara Atterby, Stefan Börjesson, Sofia Ny, Josef D Järhult, Sara Byfors, Jonas Bonnedahl
ESBL-producing bacteria are present in wildlife and the environment might serve as a resistance reservoir. Wild gulls have been described as frequent carriers of ESBL-producing E. coli strains with genotypic characteristics similar to strains found in humans. Therefore, potential dissemination of antibiotic resistance genes and bacteria between the human population and wildlife need to be further investigated. Occurrence and characterization of ESBL-producing E. coli in Swedish wild gulls were assessed and compared to isolates from humans, livestock and surface water collected in the same country and similar time-period...
2017: PloS One
https://www.readbyqxmd.com/read/29277529/comparative-genomics-of-the-rmtb-carrying-inci1-st136-plasmids-in-avian-escherichia-coli-isolates-from-chickens-in-china
#6
Li Yuan, Jian-Hua Liu, Xiang-Dang Du, Zhi-Yong Zong, Min Chen, Gong-Zheng Hu, Yu-Shan Pan
Eight rmtB-carrying avian Escherichia coli strains from a farm in China were characterized in our previous study, but little is known about the backbones and entire multiresistance regions (MRRs) of these plasmids. Here, three rmtB-carrying IncI1 ST136 plasmids were analyzed by whole-plasmid sequencing and compared. These plasmids were composed of an 83,470-bp IncI1 backbone carrying genes responsible for plasmid replication, transfer, maintenance, and stability functions, and a 17,330-bp MRR for pEC006 and pEC007, and a 34,626-bp MRR for pEC008...
December 22, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29238335/treatment-with-cefotaxime-affects-expression-of-conjugation-associated-proteins-and-conjugation-transfer-frequency-of-an-inci1-plasmid-in-escherichia-coli
#7
Thea S B Møller, Gang Liu, Anders Boysen, Line E Thomsen, Freja L Lüthje, Sisse Mortensen, Jakob Møller-Jensen, John E Olsen
Horizontal gene transfer (HGT) is the major mechanism responsible for spread of antibiotic resistance. Antibiotic treatment has been suggested to promote HGT, either by directly affecting the conjugation process itself or by selecting for conjugations subsequent to DNA transfer. However, recent research suggests that the effect of antibiotic treatment on plasmid conjugation frequencies, and hence the spread of resistance plasmids, may have been overestimated. We addressed the question by quantifying transfer proteins and conjugation frequencies of a blaCTX-M-1 encoding IncI1 resistance plasmid in Escherichia coli MG1655 in the presence and absence of therapeutically relevant concentrations of cefotaxime (CTX)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29227186/diversity-of-ctx-m-extended-spectrum-%C3%AE-lactamases-in-escherichia-coli-isolates-from-retail-raw-ground-beef-first-report-of-ctx-m-24-and-ctx-m-32-in-algeria
#8
Nesrine Rebbah, Yamina Messai, Pierre Châtre, Marisa Haenni, Jean Yves Madec, Rabah Bakour
The aim of this study was to investigate the prevalence and molecular features of extended-spectrum cephalosporin resistance in Escherichia coli isolates contaminating ground beef at retail in Algeria. Of 371 ground beef samples, 27.5% were found to contain cefotaxime-resistant E. coli isolates distributed into A (24.5%), B1 (60.8%), and D (14.7%) phylogroups. A rate of 88.2% of isolates had a multidrug-resistance phenotype. All strains were producers of CTX-M type extended-spectrum β-lactamases (ESBLs): CTX-M-1, CTX-M-3, CTX-M-14, CTX-M-15, CTX-M-24, or CTX-M-32...
December 11, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29185858/antimicrobial-resistance-in-escherichia-coli-isolated-from-wild-animals-in-poland
#9
Dariusz Wasyl, Magdalena Zając, Anna Lalak, Magdalena Skarżyńska, Ilona Samcik, Renata Kwit, Artur Jabłoński, Łukasz Bocian, Grzegorz Woźniakowski, Andrzej Hoszowski, Krzysztof Szulowski
Antimicrobial resistance was tested in Escherichia coli isolated from feces (n = 660) of red deer, roe deer, fallow deer, European bison, and wild boar shot in regional forests in Poland during two winter hunting seasons. Indicator E. coli (n = 542) was resistant against 11 of 14 tested compounds, mostly sulfamethoxazole, streptomycin, ampicillin, trimethoprim, and tetracycline (1.3-6.6% range). No significant differences were observed between boar and ruminant isolates. Most of deer and bison isolates showed no resistance...
November 29, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29116070/-the-importance-of-c-terminal-aspartic-acid-residue-d141-to-the-antirestriction-activity-of-the-ardb-r64-protein
#10
A A Kudryavtseva, M S Osetrova, V Ya Livinyuk, I V Manukhov, G B Zavilgelsky
Antirestriction proteins of the ArdB/KlcA family are specific inhibitors of restriction (endonuclease) activity of type-I restriction/modification enzymes. The effect of conserved amino acid residues on the antirestriction activity of the ArdB protein encoded by the transmissible R64 (IncI1) plasmid has been investigated. An analysis of the amino acid sequences of ArdB homologues demonstrated the presence of four groups of conserved residues ((1) R16, E32, and W51; (2) Y46 and G48; (3) S81, D83 and E132, and (4) N77, L(I)140, and D141) on the surface of the protein globule...
September 2017: Molekuliarnaia Biologiia
https://www.readbyqxmd.com/read/29102123/convergence-of-plasmid-architectures-drives-emergence-of-multi-drug-resistance-in-a-clonally-diverse-escherichia-coli-population-from-a-veterinary-clinical-care-setting
#11
Sam Wagner, Nadejda Lupolova, David L Gally, Sally A Argyle
The purpose of this study was to determine the plasmid architecture and context of resistance genes in multi-drug resistant (MDR) Escherichia coli strains isolated from urinary tract infections in dogs. Illumina and single-molecule real-time (SMRT) sequencing were applied to assemble the complete genomes of E. coli strains associated with clinical urinary tract infections, which were either phenotypically MDR or drug susceptible. This revealed that multiple distinct families of plasmids were associated with building an MDR phenotype...
November 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28963220/draft-genome-sequences-of-salmonella-enterica-isolates-containing-incompatibility-group-i1-plasmids-from-swine-poultry-and-human-sources
#12
Pravin R Kaldhone, Bijay K Khajanchi, Jing Han, Rajesh Nayak, Steven C Ricke, Steven L Foley
The draft genome sequences of eight Salmonella enterica isolates from various sources were evaluated for the influence of incompatibility group I1 (IncI1) plasmids on virulence. Strains SE142, SE143, SE144, and SE146 originated from swine, SE36N and SE89N from poultry-related sources, and SE991 and SE1148 from human patients.
September 28, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28939468/complete-nucleotide-sequence-of-psklx3330-an-inci1-plasmid-carrying-blactx-m-55-isolated-from-community-onset-escherichia-coli-infection
#13
LETTER
Beiwen Zheng, Jing Zhang, Xiawei Jiang, Hong Cheng, Jinru Ji, Yonghong Xiao, Lanjuan Li
No abstract text is available yet for this article.
September 19, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28919197/imported-poultry-meat-as-a-source-of-extended-spectrum-cephalosporin-resistant-cmy-2-producing-salmonella-heidelberg-and-s-minnesota-in-european-union-2014-2015
#14
Joana Campos, Joana Mourão, Leonor Silveira, Margarida Saraiva, Cristina Belo Correia, Ana Paula Maçãs, Luísa Peixe, Patrícia Antunes
Extended-spectrum cephalosporin (ESC)-resistant Salmonella has been described at low level in EU, nevertheless the increasing importation of poultry meat could be an important source of epidemic strains carrying ESC-resistant genes. We evaluated ESC resistance and characterized genetic platforms as well as the clonal relatedness of Salmonella isolates from poultry meat products imported into Portugal. All Salmonella isolates recovered from samples of fresh meat destined to be imported into the EU in the scope of Portuguese official border control between 2014 and 2015 were studied...
September 14, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28887202/hospital-sewage-water-a-reservoir-for-variants-of-new-delhi-metallo-%C3%AE-lactamase-blandm-and-esbl-producing-enterobacteriaceae
#15
Shadab Parvez, Asad U Khan
New Delhi metallo-β-lactamase (NDM)-producing Enterobacteriaceae has become a threat to public health. Hospital sewage is generally unexplored, having a potential for harbouring bacteria causing healthcare-associated infections. Hence, we initiated this study to monitor NDM-producing Enterobacteriaceae in hospital sewage water. We detected 32 isolates with blaNDM variants, 17 Escherichia coli, 8 Citrobacter freundii, 4 Shigella boydii, 2 Citrobacter braakii, and 1 Citrobacter farmeri in hospital sewage water, showing resistance to all antibiotics except colistin...
September 5, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28873943/novel-sequence-types-of-extended-spectrum-and-acquired-ampc-beta-lactamase-producing-escherichia-coli-and-escherichia-clade-v-isolated-from-wild-mammals
#16
Carla Andrea Alonso, Leticia Alcalá, Carmen Simón, Carmen Torres
The closer contact with wildlife due to the growing human population and the destruction of natural habitats emphasizes the need of gaining insight into the role of animals as source of antimicrobial resistance. Here, we aim at characterizing the antimicrobial resistance genes and phylogenetic distribution of commensal Escherichia coli from 62 wild mammals. Isolates exhibiting resistance to ≥1 antibiotic were detected in 25.8% of the animals and 6.4% carried an extended-spectrum beta-lactamase (ESBL)/AmpC-producing E...
August 1, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/28802274/horizontal-dissemination-of-antimicrobial-resistance-determinants-in-multiple-salmonella-serotypes-following-isolation-from-the-commercial-swine-operation-environment-after-manure-application
#17
Suchawan Pornsukarom, Siddhartha Thakur
The aim of this study was to characterize the plasmids carrying antimicrobial resistance (AMR) determinants in multiple Salmonella serotypes recovered from the commercial swine farm environment after manure application on land. Manure and soil samples were collected on day 0 before and after manure application on six farms in North Carolina, and sequential soil samples were recollected on days 7, 14, and 21 from the same plots. All environmental samples were processed for Salmonella, and their plasmid contents were further characterized...
October 15, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28799283/selection-and-transfer-of-an-inci1-tet-a-plasmid-of-escherichia-coli-in-an-ex%C3%A2-vivo-model-of-the-porcine-caecum-at-doxycycline-concentrations-caused-by-crosscontaminated-feed
#18
L E J Peeters, T De Mulder, E Van Coillie, J Huygens, A Smet, E Daeseleire, J Dewulf, H Imberechts, P Butaye, F Haesebrouck, S Croubels, M Heyndrickx, G Rasschaert
AIMS: The aim of this study was to investigate the effect of subtherapeutic intestinal doxycycline (DOX) concentrations (4 and 1 mg l(-1) ), caused by cross-contamination of feed, on the enrichment of a DOX-resistant commensal Escherichia coli and its resistance plasmid in an ex vivo model of the porcine caecum. METHODS AND RESULTS: A DOX-resistant, tet(A)-carrying, porcine commensal E. coli strain (EC 682) was cultivated for 6 days in the porcine caecum model under different conditions (0, 1 and 4 mg l(-1) DOX)...
August 11, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28728058/high-prevalence-of-esbls-in-retail-chicken-meat-despite-reduced-use-of-antimicrobials-in-chicken-production-france
#19
Tiago Casella, Mara Correa Lelles Nogueira, Estelle Saras, Marisa Haenni, Jean-Yves Madec
Extended-spectrum cephalosporins (ESCs) are critically important antibiotics for humans and their use in animals poses a potential threat for public health. Chicken represents an increasing part of the human diet and has also been regarded as a source of ESC-resistant Enterobacteriaceae because of the worldwide off-label use of ceftiofur, a broad-spectrum cephalosporin. Thus, numerous studies pointed out chicken as a reservoir of ESBL/pAmpC genes, plasmids and/or clones at risk for humans. In France, late 2011, strong political pressure led to a drastic reduction of ceftiofur use and all other antibiotics in chicken production...
July 10, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28689895/plasmid-mediated-transfer-of-ctx-m-55-extended-spectrum-beta-lactamase-among-different-strains-of-salmonella-and-shigella-spp-in-the-republic-of-korea
#20
Jin Seok Kim, Soojin Kim, Jungsun Park, Eunkyung Shin, Young-Sun Yun, Deog-Yong Lee, Hyo-Sun Kwak, Won Keun Seong, Gyung Tae Chung, Junyoung Kim
We screened 10 CTX-M-55-producing Shigella and Salmonella isolates from a national surveillance in Korea. The blaCTX-M-55 was located on the IncI1 (n=5), IncA/C (n=4) and IncZ (n=1) plasmids, downstream of ISEcp1, IS26-ISEcp1 and ISEcp1-IS5 sequences, respectively. These results indicate that CTX-M-55 has disseminated to other bacteria by lateral plasmid transfer.
March 30, 2017: Diagnostic Microbiology and Infectious Disease
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