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https://www.readbyqxmd.com/read/28338012/prevalence-of-st1193-clone-and-inci1-st16-plasmid-in-e-coli-isolates-carrying-blactx-m-55-gene-from-urinary-tract-infections-patients-in-china
#1
Liang Xia, Yang Liu, Shu Xia, Timothy Kudinha, Shu-Nian Xiao, Nan-Shan Zhong, Guo-Sheng Ren, Chao Zhuo
To study molecular epidemiology of CTX-M-55-carrying Escherichia coli isolates from urinary tract infections (UTIs) in China. 111 blaCTX-M-55-positive E.coli isolates from UTIs patients in China were studied. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were used to analyze the homologies among the strains. Conjugation experiments, S1nuclease PFGE and PCR analysis were performed to characterize plasmids harboring blaCTX-M-55 and their genetic environment. 111 isolates were clustered into 86 individual pulsotypes and three clusters by PFGE...
March 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28333298/occurrence-and-molecular-characteristics-of-esbl-ampc-producing-escherichia-coli-in-faecal-samples-from-horses-in-an-equine-clinic
#2
Ilias Apostolakos, Eelco Franz, Angela H A M van Hoek, Alice Florijn, Christiaan Veenman, Marianne M Sloet-van Oldruitenborgh-Oosterbaan, Cindy Dierikx, Engeline van Duijkeren
Objectives: To investigate the occurrence and characteristics of ESBL/AmpC-producing Escherichia coli in faecal samples from horses at one equine clinic in the Netherlands. Methods: A total of 91 horses, including residents and patients, were sampled. ESBL/AmpC-producing E. coli were identified by a combination disc diffusion test. Phylogenetic groups and MLST were determined. ESBL/AmpC genes were analysed using PCR and sequencing. Plasmids were characterized by transformation and PCR-based replicon typing...
March 13, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28333184/analysis-of-blashv-12-carrying-escherichia-coli-clones-and-plasmids-from-human-animal-and-food-sources
#3
Carla Andrea Alonso, Geovana Brenner Michael, Jun Li, Sergio Somalo, Carmen Simón, Yang Wang, Heike Kaspar, Kristina Kadlec, Carmen Torres, Stefan Schwarz
Objectives: This study aimed at characterizing 23 Escherichia coli isolates from various sources and their respective bla SHV-12 -carrying plasmids and sequencing one of these plasmids completely. Methods: Isolates were typed by XbaI-PFGE, MLST and PCR-based phylotyping. Transformed bla SHV-12 -carrying plasmids were examined by replicon typing, S1-nuclease, conjugation, EcoRI-HindIII-BamHI digests and plasmid MLST. Co-located resistance genes and integrons as well as the bla SHV-12 genetic environment were analysed by PCR and sequencing...
February 17, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28276133/plasmid-mediated-resistance-to-cephalosporins-and-quinolones-in-escherichia-coli-from-american-crows-in-the-usa
#4
Ivana Jamborova, Monika Dolejska, Ludek Zurek, Andrea K Townsend, Anne B Clark, Julie C Ellis, Ivo Papousek, Alois Cizek, Ivan Literak
American crow (Corvus brachyrhynchos) feces were tested for Escherichia coli with plasmid-mediated quinolone resistance (PMQR), extended-spectrum beta-lactamases (ESBL) and AmpC beta-lactamases. A total of 590 fecal samples were collected at four roosting sites in the USA and cultivated on selective media. Pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) were performed to assess clonality. Transferability of resistance genes was studied using conjugation and transformation bioassays...
March 9, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28243225/prevalence-and-molecular-characteristics-of-extended-spectrum-%C3%AE-lactamase-genes-in-escherichia-coli-isolated-from-diarrheic-patients-in-china
#5
Li Bai, Lili Wang, Xiaorong Yang, Juan Wang, Xin Gan, Wei Wang, Jin Xu, Qian Chen, Ruiting Lan, Séamus Fanning, Fengqin Li
Background: The emergence and spread of antimicrobial resistance has become a major global public health concern. A component element of this is the spread of the plasmid-encoded extended-spectrum b-lactamase (ESBL) genes, conferring resistance to third-generation cephalosporins. The purpose of this study was to investigate the molecular characteristics of ESBL-encoding genes identified in Escherichia coli cultured from diarrheic patients in China from 2013 to 2014. Materials and Methods: A total of 51 E. coli were confirmed as ESBL producers by double-disk synergy testing of 912 E...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28208218/antibiotic-resistance-in-escherichia-coli-in-husbandry-animals-the-african-perspective
#6
REVIEW
Carla Andrea Alonso, Myriam Zarazaga, Rym Ben Sallem, Ahlem Jouini, Karim Ben Slama, Carmen Torres
In the last years different surveillances have been published in Africa, especially in Northern countries, regarding antimicrobial resistance among husbandry animals. Information is still scarce, but the available data shows a worrying picture. Although the highest resistance rates have been described against tetracycline, penicillins and sulphonamides, prevalence of plasmid mediated quinolone resistance genes and extended-spectrum β-lactamase (ESBL) are being increasingly reported. Among ESBLs, the CTX-M-1 group was dominant in most African surveys, being CTX-M-15 the main variant both in animals and humans, except in Tunisia where CTX-M-1 was more frequently detected among E...
February 16, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28137797/a-whole-genome-sequencing-approach-to-study-cefoxitin-resistant-salmonella-enterica-serovar-heidelberg-from-various-sources
#7
Romaine Edirmanasinghe, Rita Finley, E Jane Parmley, Brent P Avery, Carolee Carson, Sadjia Bekal, George Golding, Michael R Mulvey
This study characterized cefoxitin-resistant and susceptible Salmonella enterica serovar Heidelberg from human, abattoir poultry, and retail poultry to assess the molecular relationships of isolates from these sources in Québec in 2012. Isolates were collected as part of the Canadian Integrated Program for Antimicrobial Resistance Surveillance (CIPARS). All isolates were subjected to antimicrobial susceptibility testing, polymerase chain reaction (PCR) for CMY-2, pulsed-field gel electrophoresis (PFGE), and whole genome sequencing (WGS)...
January 30, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28125687/high-heterogeneity-of-escherichia-coli-sequence-types-harbouring-esbl-ampc-genes-on-inci1-plasmids-in-the-colombian-poultry-chain
#8
Luis Ricardo Castellanos, Pilar Donado-Godoy, Maribel León, Viviana Clavijo, Alejandra Arevalo, Johan F Bernal, Arjen J Timmerman, Dik J Mevius, Jaap A Wagenaar, Joost Hordijk
BACKGROUND: Escherichia coli producing ESBL/AmpC enzymes are unwanted in animal production chains as they may pose a risk to human and animal health. Molecular characterization of plasmids and strains carrying genes that encode these enzymes is essential to understand their local and global spread. OBJECTIVES: To investigate the diversity of genes, plasmids and strains in ESBL/AmpC-producing E. coli from the Colombian poultry chain isolated within the Colombian Integrated Program for Antimicrobial Resistance Surveillance (Coipars)...
2017: PloS One
https://www.readbyqxmd.com/read/27938421/high-prevalence-of-fosfomycin-resistance-gene-fosa3-in-bla-ctx-m-harbouring-escherichia-coli-from-urine-in-a-chinese-tertiary-hospital-during-2010-2014
#9
X-L Cao, H Shen, Y-Y Xu, X-J Xu, Z-F Zhang, L Cheng, J-H Chen, Y Arakawa
Fosfomycin has become a therapeutic option in urinary tract infections. We identified 57 fosfomycin-resistant Escherichia coli from 465 urine-derived extended-spectrum β-lactamase (ESBL)-producing isolates from a Chinese hospital during 2010-2014. Of the 57 fosfomycin-resistant isolates, 51 (89·5%) carried fosA3, and one carried fosA1. Divergent pulsed-field gel electrophoresis profiles and multi-locus sequence typing results revealed high clonal diversity in the fosA3-positive isolates. Conjugation experiments showed that the fosA3 genes from 50 isolates were transferable, with IncFII or IncI1 being the most prevalent types of plasmids...
December 12, 2016: Epidemiology and Infection
https://www.readbyqxmd.com/read/27828730/characterization-of-resistance-genes-and-plasmids-from-outbreaks-and-illness-clusters-caused-by-salmonella-resistant-to-ceftriaxone-in-the-united-states-2011-2012
#10
Jason P Folster, Julian E Grass, Amelia Bicknese, Julia Taylor, Cindy R Friedman, Jean M Whichard
Salmonella is an important cause of foodborne illness; however, quickly identifying the source of these infections can be difficult, and source identification is a crucial step in preventing additional illnesses. Although most infections are self-limited, invasive salmonellosis may require antimicrobial treatment. Ceftriaxone, an extended-spectrum cephalosporin, is commonly used for treatment of salmonellosis. Previous studies have identified a correlation between the food animal/retail meat source of ceftriaxone-resistant Salmonella and the type of resistance gene and plasmid it carries...
March 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/27826018/analysis-of-pcerc7-a-small-antibiotic-resistance-plasmid-from-a-commensal-st131-escherichia-coli-defines-a-diverse-group-of-plasmids-that-include-various-segments-adjacent-to-a-multimer-resolution-site-and-encode-the-same-nika-relaxase-accessory-protein-enabling
#11
Robert A Moran, Ruth M Hall
The ampicillin resistance plasmid pCERC7, carrying transposon Tn2 with an IS4 insertion, was detected in the draft genome of a commensal Escherichia coli isolate. The genome data also revealed that this isolate belongs to ST131, clade B. pCERC7 is 9712bp comprised of a 3319bp backbone, Tn2::IS4 (6388bp) and 5bp of target site duplication, and was present at a copy number of 40. pCERC7 is related to several plasmids composed of only the backbone, or the backbone with the Tn2 insertion in the same position. These plasmids have been found previously in Escherichia coli or Salmonella enterica recovered in several different countries from as early as the 1970s...
November 5, 2016: Plasmid
https://www.readbyqxmd.com/read/27821452/ctx-m-190-a-novel-%C3%AE-lactamase-resistant-to-tazobactam-and-sulbactam-identified-in-an-escherichia-coli-clinical-isolate
#12
Zhen Shen, Baixing Ding, Yingmin Bi, Shi Wu, Su Xu, Xiaogang Xu, Qinglan Guo, Minggui Wang
A novel β-lactamase, CTX-M-190, derived from CTX-M-55 by a single substitution of Ser for Thr at position 133 (Ser133Thr), was identified in a natural Escherichia coli clinical isolate. CTX-M-190 exhibited potent hydrolytic activity against cefotaxime, with a kcat/Km ratio of 14.5 μM(-1) s(-1), and was highly resistant to inhibition by the β-lactamase inhibitors tazobactam and sulbactam, whose 50% inhibitory concentrations were 77- and 55-fold higher, respectively, for CTX-M-190 than for CTX-M-55. blaCTX-M-190 was located within the genetic platform ISEcp1-blaCTX-M-orf477, which was harbored by a 70-kb IncI1 plasmid...
January 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27795239/draft-genome-sequence-of-an-escherichia-coli-strain-isolated-from-a-gallus-gallus-broiler-producing-the-novel-ctx-m-166-variant
#13
Vera Manageiro, Lurdes Clemente, Sílvia Duarte, Luís Vieira, Manuela Caniça
We report here the draft genome sequence of the CTX-M-166-harboring O6:H16 sequence type 48 (ST48)-fimH34 Escherichia coli strain recovered from a Gallus gallus broiler. Sequence analyses revealed the presence of an IncI1/ST103-ISEcp1-blaCTX-M-166-orf477 plasmid region and of diverse antibiotic resistance and virulence-acquired genes.
October 6, 2016: Genome Announcements
https://www.readbyqxmd.com/read/27773543/high-virulence-cmy-2-and-ctx-m-2-producing-avian-pathogenic-escherichia-coli-strains-isolated-from-commercial-turkeys
#14
Ketrin Cristina da Silva, Marcos Paulo Vieira Cunha, Louise Cerdeira, Maria Gabriela Xavier de Oliveira, Mirela Caroline Vilela de Oliveira, Cleise Ribeiro Gomes, Nilton Lincopan, Terezinha Knöbl, Andrea Micke Moreno
This study reports the high-virulence phylogenetic backgrounds of CMY-2- and CTX-M-2-producing avian pathogenic Escherichia coli strains isolated from turkeys sent to slaughter and condemned by airsacculitis in Brazil. Among 300 air sac samples, seven E. coli strains produced plasmid-mediated CMY-2-type AmpC, of which three carried also the blaCTX-M-2 Extended Spectrum Beta-Lactamase encoding gene. Interestingly, the transfer of the blaCMY-2 gene was positive for three E. coli strains, being associated with the presence of IncI1 plasmids...
January 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27746775/inchi2-plasmids-are-predominant-in-antibiotic-resistant-salmonella-isolates
#15
Wenyao Chen, Tingzi Fang, Xiujuan Zhou, Daofeng Zhang, Xianming Shi, Chunlei Shi
The wide usage of antibiotics contributes to the increase in the prevalence of antibiotic-resistant Salmonella. Plasmids play a critical role in horizontal transfer of antibiotic resistance markers in Salmonella. This study aimed to screen and characterize plasmid profiles responsible for antibiotic resistance in Salmonella and ultimately to clarify the molecular mechanism of transferable plasmid-mediated antibiotic resistance. A total of 226 Salmonella isolates were examined for antimicrobial susceptibility by a disk diffusion method...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27671066/whole-genome-sequencing-identifies-in-vivo-acquisition-of-a-blactx-m-27-carrying-incfii-transmissible-plasmid-as-the-cause-of-ceftriaxone-treatment-failure-for-an-invasive-salmonella-enterica-serovar-typhimurium-infection
#16
Bruce McCollister, Cassandra V Kotter, Daniel N Frank, Taylor Washburn, Michael G Jobling
We report a case of ceftriaxone treatment failure for bacteremia caused by Salmonella enterica subsp. enterica serovar Typhimurium, due to the in vivo acquisition of a blaCTX-M-27-encoding IncFII group transmissible plasmid. The original β-lactamase-susceptible isolate ST882S was replaced by the resistant isolate ST931R during ceftriaxone treatment. After relapse, treatment was changed to ciprofloxacin, and the patient recovered. Isolate ST931R could transfer resistance to Escherichia coli at 37°C. We used whole-genome sequencing of ST882S and ST931R, the E...
December 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27566885/detection-of-plasmid-borne-extended-spectrum-%C3%AE-lactamase-esbl-genes-in-escherichia-coli-isolates-from-bovine-mastitis
#17
Christin Freitag, Geovana Brenner Michael, Kristina Kadlec, Melanie Hassel, Stefan Schwarz
Extended-spectrum β-lactamase (ESBL)-producing isolates have been increasingly reported during recent years. The aims of this study were to characterize ESBL-producing Escherichia coli from bovine mastitis as well as their ESBL gene-carrying plasmids. A culture collection of E. coli isolated from bovine quarter milk samples (2009-2013), was screened for ESBL production using ESBL selective agar plates. Putative ESBL producers (n=16) were investigated by phenotypic confirmatory tests and were characterized by the detection/sequencing of ESBL genes, XbaI macrorestriction analysis, multilocus sequence typing (MLST), phylotyping and antimicrobial susceptibility testing...
August 16, 2016: Veterinary Microbiology
https://www.readbyqxmd.com/read/27550353/sequence-type-48-escherichia-coli-carrying-the-blactx-m-1-inci1-st3-plasmid-in-drinking-water-in-france
#18
Jean-Yves Madec, Marisa Haenni, Cécile Ponsin, Nicolas Kieffer, Emmanuelle Rion, Benoit Gassilloud
Drinking water has rarely been recognized as a source of antimicrobial resistance for humans, and only in low-income countries. Here, a sequence type 48 Escherichia coli isolate carrying the blaCTX-M-1 IncI1/ST3 plasmid was recovered from drinking water in France. This plasmid was similar to other blaCTX-M-1 IncI1/ST3 plasmids found previously in animals and humans. Our findings highlight the possible human transfer of extended-spectrum β-lactamase (ESBL) genes through drinking water in high-income countries...
October 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27497122/isolation-and-characterization-of-antimicrobial-resistant-nontyphoidal-salmonella-enterica-serovars-from-imported-food-products
#19
Dongryeoul Bae, Ohgew Kweon, Ashraf A Khan
The objective of this study was to determine antimicrobial resistance and elucidate the resistance mechanism in nontyphoidal Salmonella enterica serovars isolated from food products imported into the United States from 2011 to 2013. Food products contaminated with antimicrobial-resistant nontyphoidal S. enterica were mainly imported from Taiwan, Indonesia, Vietnam, and China. PCR, DNA sequencing, and plasmid analyses were used to characterize antimicrobial resistance determinants. Twentythree of 110 S. enterica isolates were resistant to various antimicrobial classes, including β-lactam, aminoglycoside, phenicol, glycopeptide, sulfonamide, trimethoprim, and/or fluoroquinolone antimicrobial agents...
August 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/27494914/molecular-characterization-of-blaesbl-producing-escherichia-coli-cultured-from-pig-farms-in-ireland
#20
Juan Wang, James F Gibbons, Kathleen McGrath, Li Bai, Fengqin Li, Finola C Leonard, Roger Stephan, Séamus Fanning
OBJECTIVES: To characterize ESBL-encoding Escherichia coli cultured from pigs and their plasmids carrying these genes following conjugation into recipient strains. METHODS: Six ESBL-producing E. coli were recovered from faecal samples taken from pigs along with a further isolate from the environment of a farrowing house on three pig farms in Ireland. These isolates were characterized by phylogenetic grouping, MLST and ESBL genotype analyses. Conjugation experiments were carried out in broth mating assays...
November 2016: Journal of Antimicrobial Chemotherapy
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