keyword
MENU ▼
Read by QxMD icon Read
search

Tet proteins

keyword
https://www.readbyqxmd.com/read/29908567/construction-of-an-improved-aspergillus-niger-platform-for-enhanced-glucoamylase-secretion
#1
Markus R M Fiedler, Lars Barthel, Christin Kubisch, Corrado Nai, Vera Meyer
BACKGROUND: The lifestyle of filamentous fungi depends on the secretion of hydrolytic enzymes into the surrounding medium, which degrade polymeric substances into monomers that are then taken up to sustain metabolism. This feature has been exploited in biotechnology to establish platform strains with high secretory capacity including Aspergillus niger. The accepted paradigm is that proteins become mainly secreted at the tips of fungal hyphae. However, it is still a matter of debate if the amount of growing hyphal tips in filamentous fungi correlates with an increase in secretion, with previous studies showing either a positive or no correlation...
June 16, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29905846/5-formylcytosine-mediated-dna-protein-cross-links-block-dna-replication-and-induce-mutations-in-human-cells
#2
Shaofei Ji, Iwen Fu, Spandana Naldiga, Hongzhao Shao, Ashis K Basu, Suse Broyde, Natalia Y Tretyakova
5-Formylcytosine (5fC) is an epigenetic DNA modification introduced via TET protein-mediated oxidation of 5-methyl-dC. We recently reported that 5fC form reversible DNA-protein conjugates (DPCs) with histone proteins in living cells (Ji et al. (2017) Angew. Chem. Int. Ed., 56:14130-14134). We now examined the effects of 5fC mediated DPCs on DNA replication. Synthetic DNA duplexes containing site-specific DPCs between 5fC and lysine-containing proteins and peptides were subjected to primer extension experiments in the presence of human translesion synthesis DNA polymerases η and κ...
June 14, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29898922/epigenetic-alterations-mediate-ipsc-normalization-of-dna-repair-expression-and-tnr-stability-in-huntington-s-disease
#3
Peter A Mollica, Martina Zamponi, John A Reid, Deepak K Sharma, Alyson E White, Roy C Ogle, Robert D Bruno, Patrick C Sachs
Huntington's disease (HD) is a rare autosomal dominant neurodegenerative disorder caused by a cytosine-adenine-guanine (CAG) trinucleotide repeat (TNR) expansion within the HTT gene. The mechanisms underlying HD-associated cellular dysfunction during pluripotency and neurodevelopment, are poorly understood. Here we tested the hypothesis that hypomethylation during cellular reprogramming leads to up-regulation of DNA repair genes and stabilization of TNRs in HD cells. We sought to determine how the HD TNR region is affected by global epigenetic changes through cellular reprogramming and early neurodifferentiation...
June 13, 2018: Journal of Cell Science
https://www.readbyqxmd.com/read/29884649/impaired-dna-demethylation-of-c-ebp-sites-causes-premature-aging
#4
Andrea Schäfer, Bernadette Mekker, Medhavi Mallick, Viviana Vastolo, Emil Karaulanov, Dominik Sebastian, Carina von der Lippen, Bernd Epe, Damien J Downes, Carola Scholz, Christof Niehrs
Changes in DNA methylation are among the best-documented epigenetic alterations accompanying organismal aging. However, whether and how altered DNA methylation is causally involved in aging have remained elusive. GADD45α (growth arrest and DNA damage protein 45A) and ING1 (inhibitor of growth family member 1) are adapter proteins for site-specific demethylation by TET (ten-eleven translocation) methylcytosine dioxygenases. Here we show that Gadd45a/Ing1 double-knockout mice display segmental progeria and phenocopy impaired energy homeostasis and lipodystrophy characteristic of Cebp ( CCAAT/enhancer-binding protein ) mutants...
June 8, 2018: Genes & Development
https://www.readbyqxmd.com/read/29879840/ultrasensitive-photoelectrochemical-biosensor-based-on-dna-tetrahedron-as-nanocarrier-for-efficient-immobilization-of-cdte-qds-methylene-blue-as-signal-probe-with-near-zero-background-noise
#5
Mengjie Li, Chuan Xiong, Ying-Ning Zheng, Wen-Bin Liang, Ruo Yuan, Yaqin Chai
Usually, photoelectrochemical (PEC) assays were devoted to the direct modification of photoactive materials on sensing interface, thereby producing high initial signal and unneglected background noise, which could further result in low sensitivity and restricted detection limit during the detection of targets. In this work, a PEC biosensor with near-zero background noise was established for ultrasensitive microRNA-141 (miRNA-141) detection based on DNA tetrahedron (TET) as nanocarrier for efficient immobilization of CdTe quantum dots (QDs)-Methylene Blue (MB) (TET-QDs-MB complex) as signal probe...
June 8, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29875879/characteristic-profiles-of-dna-epigenetic-modifications-in-colon-cancer-and-its-predisposing-conditions-benign-adenomas-and-inflammatory-bowel-disease
#6
Tomasz Dziaman, Daniel Gackowski, Jolanta Guz, Kinga Linowiecka, Magdalena Bodnar, Marta Starczak, Ewelina Zarakowska, Martyna Modrzejewska, Anna Szpila, Justyna Szpotan, Maciej Gawronski, Anna Labejszo, Ariel Liebert, Zbigniew Banaszkiewicz, Maria Klopocka, Marek Foksinski, Andrzej Marszalek, Ryszard Olinski
Background: Active demethylation of 5-methyl-2'-deoxycytidine (5-mdC) in DNA occurs by oxidation to 5-(hydroxymethyl)-2'-deoxycytidine (5-hmdC) and further oxidation to 5-formyl-2'-deoxycytidine (5-fdC) and 5-carboxy-2'-deoxycytidine (5-cadC), and is carried out by enzymes of the ten-eleven translocation family (TETs 1, 2, 3). Decreased level of epigenetic DNA modifications in cancer tissue may be a consequence of reduced activity/expression of TET proteins. To determine the role of epigenetic DNA modifications in colon cancer development, we analyzed their levels in normal colon and various colonic pathologies...
2018: Clinical Epigenetics
https://www.readbyqxmd.com/read/29866801/characterization-of-a-glycyl-specific-tet-aminopeptidase-complex-from-pyrococcus-horikoshii
#7
Hind Basbous, Alexandre Appolaire, Eric Girard, Bruno Franzetti
The TET peptidases are large self-compartmentalized complexes that form dodecameric particles. These members of the M42 family metallopeptidases are widely distributed in prokaryotes. Three different versions of TET complexes with different substrate specificities were found to co-exist in the cytosol of the hyperthermophilic archaeon Pyrococcus horikoshii In the present work, we have identified a novel type of TET complex that we named PhTET4. The recombinant PhTET4 enzyme was found to self-assemble as a tetrahedral edifice similar to other TET complexes...
June 4, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29860590/inducible-promoters-and-functional-genomic-approaches-for-the-genetic-engineering-of-filamentous-fungi
#8
REVIEW
Janina Kluge, Dominik Terfehr, Ulrich Kück
In industry, filamentous fungi have a prominent position as producers of economically relevant primary or secondary metabolites. Particularly, the advent of genetic engineering of filamentous fungi has led to a growing number of molecular tools to adopt filamentous fungi for biotechnical applications. Here, we summarize recent developments in fungal biology, where fungal host systems were genetically manipulated for optimal industrial applications. Firstly, available inducible promoter systems depending on carbon sources are mentioned together with various adaptations of the Tet-Off and Tet-On systems for use in different industrial fungal host systems...
June 2, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29850538/correlation-between-the-expression-of-pd-l1-and-clinicopathological-features-in-patients-with-thymic-epithelial-tumors
#9
Yanmei Chen, Yuping Zhang, Xiaoling Chai, Jianfang Gao, Guorong Chen, Weifen Zhang, Yunxiang Zhang
The incidence of thymic epithelial tumors (TETs) in the Chinese population was much higher than that in the North American population. In clinical treatment, the prognosis of benign tumors after surgical resection was significantly better than that of malignant tumors. Currently, the commonly used clinical indicators for TET staging included Masaoka staging and WHO (2015) pathological criteria; however, the distinction between the benign and malignant tumors and diagnosis is yet to be explored. The current study demonstrated that the expression of PD-L1 in tumor cells was correlated with the degree of TET malignancy...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29849955/association-of-tet3-epigenetic-inactivation-with-head-and-neck-cancer
#10
Kiyoshi Misawa, Atsushi Imai, Daiki Mochizuki, Masato Mima, Shiori Endo, Yuki Misawa, Takeharu Kanazawa, Hiroyuki Mineta
The aim of this study was to clarify the epigenetic regulation of ten eleven translocation protein (TET) family genes, which can provide insights into the mechanisms of tumorigenesis and the risk of disease recurrence in head and neck squamous cell carcinoma (HNSCC). We generated methylation profiles of TET1 , TET2 and TET3 genes in tumor samples obtained from 233 patients with HNSCC; these included 57 hypopharynx, 44 larynx, 69 oral cavity, and 63 oropharynx tumor samples. The mRNA expression and promoter DNA methylation of TET family genes were examined via quantitative RT-PCR and methylation-specific PCR, respectively...
May 11, 2018: Oncotarget
https://www.readbyqxmd.com/read/29849932/gga-mir-130b-3p-inhibits-msb1-cell-proliferation-migration-invasion-and-its-downregulation-in-md-tumor-is-attributed-to-hypermethylation
#11
Chunfang Zhao, Xin Li, Bo Han, Lujiang Qu, Changjun Liu, Jiuzhou Song, Ling Lian, Ning Yang
Marek's disease is an oncogenic and lymphoproliferative disease of chickens caused by Marek's disease virus. Hypermethylation or hypomethylation of CpG islands in gene promoter region are involved in the initiation and progression of carcinogenesis. In this study, we analyzed differential methylation levels of upstream region of gga-miR-130b-3p gene between Marek's disease virus-infected tumorous and non-infected spleens. Around the upstream 1 kb of gga-miR-130b-3p gene, two amplicons were designed that covered 616 bp...
May 11, 2018: Oncotarget
https://www.readbyqxmd.com/read/29785070/analysis-of-antibiotics-resistant-genes-in-different-strains-of-staphylococcus-aureus
#12
Benson Otarigho, Mofolusho O Falade
The control of Staphylococcus aureus infection is being hampered by methicillin and other resistant strains. The identification of the unique antibiotic resistant genes from the genomes of various strains of S. aureus is of interest. We analyzed 11 S. aureus genomes sequences for Antibiotics Resistance Genes (ARGs) using CARD 2017 platform. We identified 32 ARGs across 11 S. aureus strains. Tet(38), norB, lmrB, mepA and mepR were present across genomes except for S. aureus strain UTSW MRSA 55. The mepA and mepR were found across 11 different genomes...
2018: Bioinformation
https://www.readbyqxmd.com/read/29773648/ten-eleven-translocation-2-demethylates-the-mmp9-promoter-and-its-down-regulation-in-preeclampsia-impairs-trophoblast-migration-and-invasion
#13
Xiaoliang Li, Chunlian Wu, Ying Shen, Ke Wang, Li Tang, Mi Zhou, Ming Yang, Tianying Pan, Xinghui Liu, Wenming Xu
Preeclampsia is the most common clinical disorder in pregnancy and might result from disordered uterine environments caused by epigenetic modifications, including deregulation of DNA methylation/demethylation. Recent research has indicated that 5-hydroxymethylcytosine (5hmC), a DNA base derived from 5-methylcytosine (5mC) via oxidation by ten-eleven translocation (TET) enzymes, is involved in DNA methylation-related plasticity. Here, we report that TET2 expression and 5hmC abundance are significantly altered in the placentas from preeclampsia patients...
May 17, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29772845/doxycycline-impairs-mitochondrial-function-and-protects-human-glioma-cells-from-hypoxia-induced-cell-death-implications-of-using-tet-inducible-systems
#14
Anna-Luisa Luger, Benedikt Sauer, Nadja I Lorenz, Anna L Engel, Yannick Braun, Martin Voss, Patrick N Harter, Joachim P Steinbach, Michael W Ronellenfitsch
Inducible gene expression is an important tool in molecular biology research to study protein function. Most frequently, the antibiotic doxycycline is used for regulation of so-called tetracycline (Tet)-inducible systems. In contrast to stable gene overexpression, these systems allow investigation of acute and reversible effects of cellular protein induction. Recent reports have already called for caution when using Tet-inducible systems as the employed antibiotics can disturb mitochondrial function and alter cellular metabolism by interfering with mitochondrial translation...
May 17, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29765910/a-promising-ifn-deficient-system-to-manufacture-ifn-sensitive-influenza-vaccine-virus
#15
Can Chen, Wenhui Fan, Jing Li, Weinan Zheng, Shuang Zhang, Limin Yang, Di Liu, Wenjun Liu, Lei Sun
Interferon (IFN)-sensitive and replication-incompetent influenza viruses are likely to be the alternatives to inactivated and attenuated virus vaccines. Some IFN-sensitive influenza vaccine candidates with modified non-structural protein 1 (NS1) are highly attenuated in IFN-competent hosts but induce robust antiviral immune responses. However, little research has been done on the manufacturability of these IFN-sensitive vaccine viruses. Here, RIG-I-knockout 293T cells were used to package the IFN-sensitive influenza A/WSN/33 (H1N1) virus expressing the mutant NS1 R38A/K41A...
2018: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29765857/dna-methylation-and-adult-neurogenesis
#16
REVIEW
Emily M Jobe, Xinyu Zhao
The role of DNA methylation in brain development is an intense area of research because the brain has particularly high levels of CpG and mutations in many of the proteins involved in the establishment, maintenance, interpretation, and removal of DNA methylation impact brain development and/or function. These include DNA methyltransferase (DNMT), Ten-Eleven Translocation (TET), and Methyl-CpG binding proteins (MBPs). Recent advances in sequencing breadth and depth as well the detection of different forms of methylation have greatly expanded our understanding of the diversity of DNA methylation in the brain...
November 9, 2017: Brain Plasticity
https://www.readbyqxmd.com/read/29757674/eicosapentaenoic-acid-induces-dna-demethylation-in-carcinoma-cells-through-a-tet1-dependent-mechanism
#17
Veronica Ceccarelli, Virginia Valentini, Simona Ronchetti, Lorenza Cannarile, Monia Billi, Carlo Riccardi, Laura Ottini, Vincenzo Nicola Talesa, Francesco Grignani, Alba Vecchini
In cancer cells, global genomic hypomethylation is found together with localized hypermethylation of CpG islands within the promoters and regulatory regions of silenced tumor suppressor genes. Demethylating agents may reverse hypermethylation, thus promoting gene re-expression. Unfortunately, demethylating strategies are not efficient in solid tumor cells. DNA demethylation is mediated by ten-eleven translocation enzymes (TETs). They sequentially convert 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), which is associated with active transcription; 5-formylcytosine; and finally, 5-carboxylcytosine...
May 14, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29751537/-mcpip3-as-a-potential-metastasis-suppressor-gene-in-human-colorectal-cancer
#18
Fat-Moon Suk, Chi-Ching Chang, Ren-Jye Lin, Shyr-Yi Lin, Ya-Ting Chen, Yu-Chih Liang
Monocyte chemotactic protein induced protein 3 (MCPIP3) belongs to the Cys⁻Cys⁻Cys⁻His (CCCH)-zinc finger protein family and contains a highly conserved CCCH-zinc finger domain and a Nedd4-BP1 YacP nuclease (NYN) domain. Previous studies showed that MCPIP3 inhibits the expression of proinflammatory genes, such as vascular cell adhesion molecule ( VCAM )-1, in human endothelial cells, but the roles and functions of MCPIP3 in cancer cells are still unknown. In human colorectal cancer specimens, we found that the messenger RNA expression of MCPIP3 was significantly downregulated in cancer tissues compared to adjacent normal tissues (18/25; average fold change of 8...
May 3, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29743892/epigenetic-regulations-in-neural-stem-cells-and-neurological-diseases
#19
REVIEW
Hang Zhou, Bin Wang, Hao Sun, Xingshun Xu, Yongxiang Wang
Among the regulatory mechanisms of the renewal and differentiation of neural stem cells, recent evidences support that epigenetic modifications such as DNA methylation, histone modification, and noncoding RNAs play critical roles in the regulation on the proliferation and differentiation of neural stem cells. In this review, we discussed recent advances of DNA modifications on the regulative mechanisms of neural stem cells. Among these epigenetic modifications, DNA 5-hydroxymethylcytosine (5hmC) modification is emerging as an important modulator on the proliferation and differentiation of neural stem cells...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29733872/experimental-and-integrative-analyses-identify-an-ets1-network-downstream-bcr-abl-in-chronic-myeloid-leukemia-cml
#20
Christophe Desterke, Maud Voldoire, Marie-Laure Bonnet, Nathalie Sorel, Sarah Pagliaro, Hind Rahban, Annelise Bennaceur Griscelli, Emilie Cayssials, Jean-Claude Chomel, Ali G Turhan
The BCR-ABL oncogene, hallmark of chronic myeloid leukemia (CML), has been shown to activate several signaling pathways in leukemic cells. The natural history of this disease has been radically modified by tyrosine kinase inhibitors (TKIs). However, resistance to several lines of TKI therapies and progression to blast crisis (BC) remain significant concerns. In order to identify novel signaling pathways induced by BCR-ABL, we performed a transcriptome analysis in BCR-ABL-expressing UT-7 cell line. More than 2000 genes differentially expressed between BCR-ABL-expressing and parental UT-7 cells were identified, and ETS1 was found to be the most up-regulated...
May 4, 2018: Experimental Hematology
keyword
keyword
20316
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"