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https://www.readbyqxmd.com/read/28229163/-prevalence-of-dog-circovirus-in-healthy-and-diarrhoeic-dogs
#1
Michaela Gentil, Achim D Gruber, Elisabeth Müller
OBJECTIVE: In 2012, a Dog circovirus (DogCV) was discovered in the USA, which was followed by further descriptions of the virus in the USA, Italy and Germany. The present study is the first to examine the prevalence of DogCV in faeces of dogs from Germany and other European countries. MATERIAL AND METHODS: Faecal samples from 184 dogs with diarrhoea and from 82 clinically healthy dogs (control group) were analysed for the presence of DogCV by PCR. Furthermore, the detection of parvovirus, coronavirus, Giardia and Cryptosporidium was performed in all samples...
February 23, 2017: Tierärztliche Praxis. Ausgabe K, Kleintiere/Heimtiere
https://www.readbyqxmd.com/read/28229045/occurrence-of-giardia-cryptosporidium-and-entamoeba-in-wild-rhesus%C3%A2-macaques-macaca-mulatta-living-in-urban-and-semi-rural-north-west-india
#2
John J Debenham, Kristoffer Tysnes, Sandhya Khunger, Lucy J Robertson
Giardia duodenalis, Cryptosporidium spp., and Entamoeba spp. are intestinal protozoa capable of infecting a range of host species, and are important causes of human morbidity and mortality. Understanding their epidemiology is important, both for public health and for the health of the animals they infect. This study investigated the occurrence of these protozoans in rhesus macaques (Macaca mulatta) in India, with the aim of providing preliminary information on the potential for transmission of these pathogens between macaques and humans...
April 2017: International Journal for Parasitology. Parasites and Wildlife
https://www.readbyqxmd.com/read/28229042/an-investigation-of-parasitic-infections-and-review-of-molecular-characterization-of-the-intestinal-protozoa-in-nonhuman-primates-in-china-from-2009-to-2015
#3
Junqiang Li, Haiju Dong, Rongjun Wang, Fuchang Yu, Yayun Wu, Yankai Chang, Chenrong Wang, Meng Qi, Longxian Zhang
Parasites are a well-known threat to nonhuman primate (NHP) populations, and potentially cause zoonotic diseases in humans. In this study, the basic data was provided of the parasites in NHPs and the molecular characterization of the Enterocytozoon bieneusi, Giardia duodenalis, Cryptosporidium spp., and Entamoeba spp. were reviewed, which were found in these samples. A total of 3349 fecal samples were collected from 34 species reared at 17 districts in zoos, farms, free-range, or research laboratories, and examined microscopically...
April 2017: International Journal for Parasitology. Parasites and Wildlife
https://www.readbyqxmd.com/read/28228066/dynamic-protein-s-palmitoylation-mediates-parasite-life-cycle-progression-and-diverse-mechanisms-of-virulence
#4
Robert W B Brown, Aabha I Sharma, David M Engman
Eukaryotic parasites possess complex life cycles and utilize an assortment of molecular mechanisms to overcome physical barriers, suppress and/or bypass the host immune response, including invading host cells where they can replicate in a protected intracellular niche. Protein S-palmitoylation is a dynamic post-translational modification in which the fatty acid palmitate is covalently linked to cysteine residues on proteins by the enzyme palmitoyl acyltransferase (PAT) and can be removed by lysosomal palmitoyl-protein thioesterase (PPT) or cytosolic acyl-protein thioesterase (APT)...
April 2017: Critical Reviews in Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28224033/distribution-and-risk-factors-associated-with-intestinal-parasite-infections-among-children-with-gastrointestinal-disorders
#5
Hamed Kiani, Ali Haghighi, Roya Salehi, Eznollah Azargashb
AIM: Prevalence and risk factors associated with intestinal parasites among children ≤ 12 years old in Nahavand county western Iran, was the objective of this search. BACKGROUND: Intestinal parasites (IPs) are important health problems among most societies. METHODS: This cross sectional study was carried out during 6 months from April to September 2014 in Nahavand County western Iran. Fecal samples were collected from 500 children suffering from gastrointestinal disorders (GIDs) and examined by macroscopy and microscopic (using saline and iodine wet mount, formalin-ether sedimentation, Trichrome and modified Ziehl Neelsen staining) methods...
December 2016: Gastroenterology and Hepatology From Bed to Bench
https://www.readbyqxmd.com/read/28224032/prevalence-of-intestinal-parasites-in-referred-individuals-to-the-medical-centers-of-tonekabon-city-mazandaran-province
#6
Samira Shahdoust, Maryam Niyyati, Ali Haghighi, Eznoallah Azargashb, Mohammad Reza Khataminejad
AIM: The aim of the present study was to determine the prevalence of intestinal parasites and their relation with socio-demographic data in referred individuals to the medical centers in Tonekabon, Mazandaran province, 2015. BACKGROUND: Due to the climatic and ecological conditions in Mazandaran province, determination of the status of intestinal parasites among referred individuals to the medical centers of Tonekabon city can help researchers and healthcare services to prevent and/or control of parasitic infection in this region...
December 2016: Gastroenterology and Hepatology From Bed to Bench
https://www.readbyqxmd.com/read/28222400/antiprotozoal-antimycobacterial-and-anti-inflammatory-evaluation-of-cnidoscolus-chayamansa-mc-vaugh-extract-and-the-isolated-compounds
#7
Mariana Z Pérez-González, Gabriel A Gutiérrez-Rebolledo, Lilián Yépez-Mulia, Irma S Rojas-Tomé, Julieta Luna-Herrera, María A Jiménez-Arellanes
Cnidoscolus chayamansa is a medicinal and edible plant known as Chaya, is commonly used as an anti-inflammatory, antiprotozoal, antibacterial agent and as a remedy for respiratory illness, gastrointestinal disorders, and vaginal infections related with the inflammation process. In this paper, we describe the plant's phytochemical analysis and biological activities (antimycobacterial, antibacterial, antiprotozoal, and anti-inflammatory properties) of the CHCl3:MeOH (1:1) leaves extract and isolated compounds, as well as the acute and sub-acute toxic effects...
February 18, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28222192/blastocystis-subtyping-and-its-association-with-intestinal-parasites-in-children-from-different-geographical-regions-of-colombia
#8
Juan David Ramírez, Carolina Flórez, Mario Olivera, María Consuelo Bernal, Julio Cesar Giraldo
Blastocystis is a common enteric protist colonizing probably more than 1 billion people with a large variety of non-human hosts. Remarkable genetic diversity has been observed, leading to the subdivision of the genus into multiple subtypes (ST), some of which are exclusively found in non-human hosts. The aim of this study was to determine the distribution of Blastocystis STs/18S alleles in symptomatic (abdominal pain, anal pruritus, diarrhea, headache, nauseas and/or vomit) and asymptomatic children from nine geographical regions of Colombia...
2017: PloS One
https://www.readbyqxmd.com/read/28219994/coinfection-with-hymenolepis-nana-hymenolepis-diminuta-giardia-intestinalis-and-human-immunodeficiency-virus-a-case-report-with-complex-immunologic-interactions
#9
Gladymar Pérez-Chacón, Leonor A Pocaterra, Elsy Rojas, Aurora Hernán, Juan Carlos Jiménez, Luz Núñez
We describe the case of a 43-year-old human immunodeficiency virus-infected man receiving combined antiretroviral therapy and coinfected with Hymenolepis nana, Hymenolepis diminuta, and Giardia intestinalis, presenting as chronic diarrhea and critical weight loss. Immunological aspects of these interactions are reviewed.
February 20, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28219812/detection-and-molecular-diversity-of-giardia-duodenalis-and-cryptosporidium-spp-in-sheltered-dogs-and-cats-in-northern-spain
#10
Horacio Gil, Lourdes Cano, Aida de Lucio, Begoña Bailo, Marta Hernández de Mingo, Guillermo A Cardona, José A Fernández-Basterra, Juan Aramburu-Aguirre, Nuria López-Molina, David Carmena
Domestic dogs and cats may act as natural reservoirs of a large number of zoonotic pathogens, including the enteric parasites Giardia duodenalis and Cryptosporidium spp., the most relevant protozoan species causing gastrointestinal disease worldwide. A cross-sectional epidemiological study aiming to assess the prevalence and molecular diversity of G. duodenalis and Cryptosporidium spp. was conducted in an animal rescue centre in the province of Álava (Northern Spain). A total of 194 and 65 faecal dropping samples from individual dogs and cats, respectively, were collected between November 2013 and June 2016...
February 17, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28216899/antiprotozoal-constituents-from-annona-cherimola-miller-a-plant-used-in-mexican-traditional-medicine-for-the-treatment-of-diarrhea-and-dysentery
#11
Fernando Calzada, Jose Correa-Basurto, Elizabeth Barbosa, David Mendez-Luna, Lilian Yepez-Mulia
BACKGROUND: Annona cherimola Miller (Annonaceae) is a medicinal plant frequently recommended in Mexican traditional medicine for the treatment of gastrointestinal disorders such as diarrhea and dysentery. OBJECTIVE: This work was undertaken to obtain information that support the traditional use of A. cherimola, on pharmacological basis using in vitro and computational experiments. MATERIAL AND METHODS: Bioassay-guided fractionation of the ethanol extract of the leaves of A...
January 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28215875/occurrence-and-molecular-genotyping-of-giardia-duodenalis-and-cryptosporidium-spp-in-wild-mesocarnivores-in-spain
#12
Marta Mateo, Marta Hernández de Mingo, Aida de Lucio, Lucía Morales, Ana Balseiro, Alberto Espí, Marta Barral, José Francisco Lima Barbero, Miguel Ángel Habela, José L Fernández-García, Rafael Calero Bernal, Pamela C Köster, Guillermo A Cardona, David Carmena
There is a surprisingly scarce amount of epidemiological and molecular data on the prevalence, frequency, and diversity of the intestinal protozoan parasites Giardia duodenalis and Cryptosporidium spp. in wildlife in general and mesocarnivore species in particular. Consequently, the extent of the cyst/oocyst environmental contamination attributable to these wild host species and their potential implications for public veterinary health remain largely unknown. In this molecular epidemiological survey a total of 193 individual faecal samples from badgers (Meles meles, n=70), ferrets (Mustela putorius furo, n=2), genets (Genetta genetta, n=6), Iberian lynxes (Lynx pardinus, n=6), beech martens (Martes foina, n=8), mongooses (Herpestes ichneumon, n=2), otters (Lutra lutra, n=2), polecats (Mustela putorius, n=2), red foxes (Vulpes vulpes, n=87), wildcats (Felis silvestris, n=2), and wolves (Canis lupus, n=6) were obtained from road-killed, hunted, and accidentally found carcasses, and from camera-trap surveys or animals entering rescue shelters, during the period December 2003-April 2016...
February 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28214721/waterborne-transmission-of-protozoan-parasites-review-of-worldwide-outbreaks-an-update-2011-2016
#13
REVIEW
Artemis Efstratiou, Jerry E Ongerth, Panagiotis Karanis
This review provides a comprehensive update of worldwide waterborne parasitic protozoan outbreaks that occurred with reports published since previous reviews largely between January 2011 and December 2016. At least 381 outbreaks attributed to waterborne transmission of parasitic protozoa were documented during this time period. The nearly half (49%) of reports occurred in New Zealand, 41% of the outbreaks in North America and 9% in Europe. The most common etiological agent was Cryptosporidium spp., reported in 63% (239) of the outbreaks, while Giardia spp...
January 25, 2017: Water Research
https://www.readbyqxmd.com/read/28209552/detection-of-giardia-duodenalis-assemblage-e-infections-at-the-tibetan-plateau-area-yaks-are-suitable-hosts
#14
Wang Guang-Hua, Wang Ge-Ping, Li Xiu-Ping, Ma Li-Qing, Gabriele Karanis, Eleni Christodoulou-Vafeiadou, Panagiotis Karanis
The prevalence of Giardia duodenalis (G. duodenalis) assemblages in yaks is poorly known. The present study examined 297 fecal samples from weaned yak, 4 to 7 months of age, from 3 different farms, in Tibetan Plateau Area (TPA) of the Qinghai Province in Western China. The prevalence of infection was determined by light and immunofluorescence microscopy, and nested-PCR. PCR was performed for the small subunit ribosomal RNA (SSU) amplified 16 positive for G. duodenalis products. The prevalence of Giardia species was 5...
February 13, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28204556/determinants-and-impact-of-giardia-infection-in-the-first-2-years-of-life-in-the-mal-ed-birth-cohort
#15
Elizabeth T Rogawski, Luther A Bartelt, James A Platts-Mills, Jessica C Seidman, Amidou Samie, Alexandre Havt, Sudhir Babji, Dixner Rengifo Trigoso, Shahida Qureshi, Sadia Shakoor, Rashidul Haque, Estomih Mduma, Samita Bajracharya, S M Abdul Gaffar, Aldo A M Lima, Gagandeep Kang, Margaret N Kosek, Tahmeed Ahmed, Erling Svensen, Carl Mason, Zulfiqar A Bhutta, Dennis R Lang, Michael Gottlieb, Richard L Guerrant, Eric R Houpt, Pascal O Bessong
No abstract text is available yet for this article.
February 15, 2017: Journal of the Pediatric Infectious Diseases Society
https://www.readbyqxmd.com/read/28197763/development-of-t-m-shift-genotyping-method-for-detection-of-cat-derived-giardia-lamblia
#16
Weida Pan, Yeqi Fu, Auwalu Yusuf Abdullahi, Mingwei Wang, Xianli Shi, Fang Yang, Xingang Yu, Xinxin Yan, Pan Zhang, Jianxiong Hang, Guoqing Li
To develop T m -shift genotyping method for detection of cat-derived Giardia lamblia, two sets of primers with two GC-rich tails of unequal length attached to their 5'-end were designed according to two SNPs (BG434 and BG170) of β-giardin (bg) gene, and specific PCR products were identified by inspection of a melting curve on real-time PCR thermocycler. A series of experiments on the stability, sensitivity, and accuracy of T m -shift method was tested, and clinical samples were also detected. The results showed that two sets of primers based on SNP could distinguish accurately between assemblages A and F...
February 15, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28192081/cryptosporidium-oocysts-and-giardia-cysts-a-practical-and-sensitive-method-for-counting-and-manipulating-small-numbers
#17
Jerry E Ongerth, Frhat M A Saaed
A rapid and inexpensive method is described for accurate and reproducible counting and manipulating small numbers of Cryptosporidium oocysts and Giardia cysts. From a suspension of oocysts or cysts at concentration from 1000 to 5000/mL (1-5/μL), replicate 5μL droplets are micro pipetted onto the edge of a microscope slide. Unstained oocysts or cysts in each droplet can be counted in a few minutes and replicated for statistical strength. The concentration of the suspension can then be verified by pipetting desired volumes containing approximately desired numbers onto confined 13mm membranes for IFA staining and counting with replication as desired...
February 10, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28182670/killing-of-diverse-eye-pathogens-acanthamoeba-spp-fusarium-solani-and-chlamydia-trachomatis-with-alcohols
#18
Yousuf Aqeel, Raquel Rodriguez, Aparajita Chatterjee, Robin R Ingalls, John Samuelson
BACKGROUND: Blindness is caused by eye pathogens that include a free-living protist (Acanthamoeba castellanii, A. byersi, and/or other Acanthamoeba spp.), a fungus (Fusarium solani), and a bacterium (Chlamydia trachomatis). Hand-eye contact is likely a contributor to the spread of these pathogens, and so hand washing with soap and water or alcohol-based hand sanitizers (when water is not available) might reduce their transmission. Recently we showed that ethanol and isopropanol in concentrations present in hand sanitizers kill walled cysts of Giardia and Entamoeba, causes of diarrhea and dysentery, respectively...
February 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28182579/synthesis-and-biological-evaluation-of-newer-1-3-4-oxadiazoles-incorporated-with-benzothiazepine-and-benzodiazepine-moieties
#19
Patel Navin, Patel Sarvil, Purohit Amit, Patel Divyesh, Rajani Dhansukh, Rosa Moo-Puc, Gildardo Rivera
A series of thiazepines and diazepines having 1,3,4-oxadiazole moiety were synthesized, and they were analyzed for their in vitro antimicrobial activity against several bacteria (Staphylococcus aureus, Staphylococcus pyogenes, Escherichia coli, and Pseudomonas aeruginosa) and fungi (Candida albicans, Aspergillus niger, and Aspergillus Clavatus) and protozoa (Entamoeba histolytica, Giardia lamblia, Trypanosoma cruzi and Leishmania mexicana). Few of the selected compounds were tested for their antitubercular activity...
February 9, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28177963/cytology-preparations-of-formalin-fixative-aid-detection-of-giardia-in-duodenal-biopsy-samples
#20
Nicole C Panarelli, Nariman Gobara, Rana S Hoda, Michael Chaump, Jose Jessurun, Rhonda K Yantiss
Giardiasis is the most common intestinal parasitic infection in the United States. The organism elicits no, or minimal, inflammatory changes in duodenal biopsy samples, so it can be easily overlooked. We performed this study to determine whether Giardia could be isolated from the formalin fixative of biopsy samples, and to evaluate the value of fluid analysis in the assessment for potential infection. We prospectively evaluated duodenal biopsy samples from 92 patients with a clinical suspicion of giardiasis or symptoms compatible with that diagnosis (ie, diarrhea, bloating, or abdominal pain) Biopsy samples were routinely processed and stained with hematoxylin and eosin...
February 7, 2017: American Journal of Surgical Pathology
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