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https://www.readbyqxmd.com/read/29040510/genomic-fragmentation-and-extrachromosomal-telomeric-repeats-impact-assessment-of-telomere-length-in-human-spermatozoa-quantitative-experiments-and-systematic-review
#1
P Kurjanowicz, S Moskovtsev, C Librach
STUDY QUESTION: Can differences in DNA isolation alter assessment of sperm telomere length (spTL) and do they account for conflicting results in the literature on spTL and male fertility? SUMMARY ANSWER: DNA isolation methods preferentially include or exclude short, extrachromosomal (EC) telomere-specific sequences that alter spTL measurements, and are responsible for a proportion of the disparity observed between investigations. WHAT IS KNOWN ALREADY: The relationship between spTL and male fertility has become an active area of research...
September 29, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29040234/preventing-wedge-deformities-when-treating-intertrochanteric-femur-fractures-with-intramedullary-devices-a-technical-tip
#2
Bennet A Butler, Ryan S Selley, Hobie D Summers, Michael D Stover
Intertrochanteric femur fractures are common injuries associated with significant patient morbidity and mortality and high societal costs. Although intramedullary devices have shown promise for treating a wide range of intertrochanteric femur fractures, improper technique can lead to malalignment, fracture displacement and/or iatrogenic fractures. In particular, a "wedge effect" has been described, in which the passage of conical reamers and the intramedullary nail itself results in distraction of intertrochanteric fragments with lateralization of the shaft from the femoral neck and varus malalignment...
October 7, 2017: Journal of Orthopaedic Trauma
https://www.readbyqxmd.com/read/29036435/the-wuschel-related-homeobox-5a-ptowox5a-is-involved-in-adventitious-root-development-in-poplar
#3
Jianbo Li, Jin Zhang, Huixia Jia, Bobin Liu, Pei Sun, Jianjun Hu, Lijuan Wang, Mengzhu Lu
Adventitious rooting is an essential step in vegetative propagation. Currently, the mechanism that regulates adventitious root (AR) development in woody plants is poorly understood. This work demonstrates that Populus tomentosa WUSCHEL-related homeobox 5a (PtoWOX5a) transcription factor is involved in AR development in poplar. PtoWOX5a was specifically expressed in the AR tip and lateral root tip during AR and lateral root regeneration from the stem segment. Phenotypic complementation experiments indicated that the PtoWOX5a can functionally complement AtWOX5 in quiescent center (QC) cells...
September 26, 2017: Tree Physiology
https://www.readbyqxmd.com/read/29036409/structure-of-an-innexin-gap-junction-channel-and-cryo-em-sample-preparation
#4
Atsunori Oshima
Gap junction channels are essential for mediating intercellular communication in most multicellular organisms. Two gene families encode gap junction channels, innexin and connexin. Although the sequence similarity between these two families based on bioinformatics is not conclusively determined, the gap junction channels encoded by these two gene families are structurally and functionally analogous. We recently reported an atomic structure of an invertebrate innexin gap junction channel using single-particle cryo-electron microscopy...
September 21, 2017: Microscopy
https://www.readbyqxmd.com/read/29036091/large-amplitude-tip-tilt-estimation-by-geometric-diversity-for-multiple-aperture-telescopes
#5
S Vievard, F Cassaing, L M Mugnier
A novel method nicknamed ELASTIC is proposed for the alignment of multiple-aperture telescopes, in particular segmented telescopes. It only needs the acquisition of two diversity images of an unresolved source, and is based on the computation of a modified, frequency-shifted, cross-spectrum. It provides a polychromatic large-range tip/tilt estimation with the existing hardware and an inexpensive noniterative unsupervised algorithm. Its performance is studied and optimized by means of simulations. They show that with 5000 photoelectrons/subaperture/frame and 1024×1024 pixel images, residues are within the capture range of interferometric phasing algorithms such as phase diversity...
August 1, 2017: Journal of the Optical Society of America. A, Optics, Image Science, and Vision
https://www.readbyqxmd.com/read/29036006/blood-perfusion-in-human-eyelid-skin-flaps-examined-by-laser-speckle-contrast-imaging-importance-of-flap-length-and-the-use-of-diathermy
#6
Cu Dinh Nguyen, Jenny Hult, Rafi Sheikh, Kajsa Tenland, Ulf Dahlstrand, Sandra Lindstedt, Malin Malmsjö
PURPOSE: It is well known that blood perfusion is important for the survival of skin flaps. As no study has been conducted to investigate how the blood perfusion in human eyelid skin flaps is affected by the flap length and diathermy, the present study was carried out to investigate these in patients. METHODS: Fifteen upper eyelids were dissected as part of a blepharoplastic procedure, releasing a 30-mm long piece of skin, while allowing the 5 mm wide distal part of the skin to remain attached, to mimic a skin flap (hereafter called a "skin flap")...
October 11, 2017: Ophthalmic Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/29035538/resolving-the-spatial-structures-of-bound-hole-states-in-black-phosphorus
#7
Zhizhan Qiu, Hanyan Fang, Alexandra Carvalho, Aleksandr S Rodin, Yanpeng Liu, Sherman Jun Rong Tan, Mykola Telychko, Pin Lv, Jie Su, Yewu Wang, Antonio H Castro Neto, Jiong Lu
Understanding the local electronic properties of individual defects and dopants in black phosphorus (BP) is of great importance for both fundamental research and technological applications. Here, we employ low-temperature scanning tunnelling microscope (LT-STM) to probe the local electronic structures of single acceptors in BP. We demonstrate that the charge state of individual acceptors can be reversibly switched by controlling the tip-induced band bending. In addition, acceptor-related resonance features in the tunnelling spectra can be attributed to the formation of Rydberg-like bound hole states...
October 16, 2017: Nano Letters
https://www.readbyqxmd.com/read/29035500/reliable-multi-state-data-storage-with-low-power-consumption-by-selective-oxidation-of-pyramid-structured-resistive-memory
#8
Youngjin Kim, Hanhyeong Choi, Hyun S Park, Moon Sung Kang, Keun-Young Shin, Sang-Soo Lee, Jong Hyuk Park
Multilevel data storage using resistive random access memory (RRAM) has attracted significant attention for addressing the challenges associated with the rapid advances in information technologies. However, it is still difficult to secure reliable multilevel resistive switching of RRAM due to the stochastic and multiple formation of conductive filaments (CFs). Herein, we demonstrate that a single CF, derived from selective oxidation by a structured Cu active electrode, can solve the reliability issue. High-quality pyramidal Cu electrodes with a sharp tip are prepared via the template-stripping method...
October 16, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29035348/air-coupled-ultrasonic-receivers-with-high-electromechanical-coupling-pmn-32-pt-strip-like-piezoelectric-elements
#9
Rymantas J Kazys, Reimondas Sliteris, Justina Sestoke
For improvement of the efficiency of air-coupled ultrasonic transducers PMN-32%PT piezoelectric crystals which possess very high piezoelectric properties may be used. The electromechanical coupling factor of such crystals for all main vibration modes such as the thickness extension and transverse extension modes is more than 0.9. Operation of ultrasonic transducers with such piezoelectric elements in transmitting and receiving modes is rather different. Therefore, for transmission and reception of ultrasonic signals, separate piezoelectric elements with different dimensions must be used...
October 16, 2017: Sensors
https://www.readbyqxmd.com/read/29035193/renal-outcome-in-iga-nephropathy-according-to-oxford-classification-and-ultrastructural-analysis-in-a-brazilian-center%C3%A2
#10
Giovana Mariani, Leandro L L Freitas, Ricardo L Zollner, Maria Almerinda V F Ribeiro Alves
AIMS: Correlate clinical and histologic features with renal outcome in patients with biopsy-proven IgA nephropathy (IgAN). MATERIALS AND METHODS: Retrospective analysis of records and renal tissue of IgAN patients. Histology was revised according to MEST score of Oxford classification. Focal segmental glomerulosclerosis (FSGS) features were assessed by light microscopy. Electron microscopy review searched for podocyte effacement. RESULTS: 67 patients were included, 56...
October 16, 2017: Clinical Nephrology
https://www.readbyqxmd.com/read/29035061/thz-nanofocusing-with-cantilevered-thz-resonant-antenna-tips
#11
Stefan Mastel, Mark Lundeberg, Pablo Alonso-Gonzalez, Yuanda Gao, Kenji Watanabe, Takashi Taniguchi, James C Hone, Frank H L Koppens, Alexey Yu Nikitin, Rainer Hillenbrand
We developed THz-resonant scanning probe tips, yielding strongly enhanced and nanoscale confined THz near fields at their tip apex. The tips with length in the order of the THz wavelength (λ = 96.5 µm) were fabricated by focused ion beam (FIB) machining and attached to standard atomic force microscopy (AFM) cantilevers. Measurements of the near-field intensity at the very tip apex (25 nm radius) as a function of tip length - via graphene-based (thermoelectric) near-field detection - reveal their first and second order geometrical antenna resonances for tip length of 33 and 78 µm, respectively...
October 16, 2017: Nano Letters
https://www.readbyqxmd.com/read/29034393/centrifugal-micropipette-tip-with-pressure-signal-readout-for-portable-quantitative-detection-of-myoglobin
#12
Yanling Song, Yuan An, Weizhi Liu, Wanfu Hou, Xingrui Li, Bingqian Lin, Zhi Zhu, Shengxiang Ge, Huang-Hao Yang, Chaoyong Yang
This paper describes a centrifugal micropipette-tip method for ELISA sample processing combined with a pressure meter for portable quantitative detection of acute myocardial infarction (AMI) biomarker myoglobin (Myo). The method enables sensitive and reliable quantification of Myo in 35 minutes. With the advantages of simplicity, speed, user friendliness, low cost, and small sample consumption, centrifugal micropipette-tip ELISA shows great potential for quantitative POC diagnostics for AMI.
October 16, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/29033615/clinical-economic-and-humanistic-burden-of-needlestick-injuries-in-healthcare-workers
#13
REVIEW
Catherine E Cooke, Jennifer M Stephens
INTRODUCTION: Needlestick injuries (NSIs) from a contaminated needle put healthcare workers (HCWs) at risk of becoming infected with a blood-borne virus and suffering serious short- and long-term medical consequences. Hypodermic injections using disposable syringes and needles are the most frequent cause of NSIs. OBJECTIVE: To perform a systematic literature review on NSI and active safety-engineered devices for hypodermic injection. METHODS: MEDLINE, EMBASE, and COCHRANE databases were searched for studies that evaluated the clinical, economic, or humanistic outcomes of NSI or active safety-engineered devices...
2017: Medical Devices: Evidence and Research
https://www.readbyqxmd.com/read/29033528/in-vitro-performance-of-diagnodent-laser-fluorescence-device-for-dental-calculus-detection-on-human-tooth-root-surfaces
#14
Thomas E Rams, Abdulaziz Y Alwaqyan
OBJECTIVE: This study assessed the reproducibility of a red diode laser device, and its capability to detect dental calculus in vitro on human tooth root surfaces. MATERIAL AND METHODS: On each of 50 extracted teeth, a calculus-positive and calculus-free root surface was evaluated by two independent examiners with a low-power indium gallium arsenide phosphide diode laser (DIAGNOdent) fitted with a periodontal probe-like sapphire tip and emitting visible red light at 655 nm wavelength...
October 2017: Saudi Dental Journal
https://www.readbyqxmd.com/read/29033081/a-cone-beam-computed-tomographic-study-of-apical-surgery-related-morphological-characteristics-of-the-distolingual-root-in-3-rooted-mandibular-first-molars-in-a-chinese-population
#15
Xiao Zhang, Ning Xu, Hanguo Wang, Qing Yu
INTRODUCTION: Apical surgery on the separate distolingual (DL) root of a 3-rooted mandibular first molar is thought to be difficult because DL roots are always severely curved, small, and shorter than distobuccal (DB) roots, and they are located far from the buccal cortical bone. The purpose of this study was to use cone-beam computed tomographic images to investigate the apical surgery-related morphological characteristics of DL roots in a Chinese population. METHODS: The screening process identified 83 subjects with 128 mandibular first molars with separate DL roots...
October 12, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/29032217/preoperative-prediction-of-the-necessity-for-anterior-clinoidectomy-during-microsurgical-clipping-of-ruptured-posterior-communicating-artery-aneurysms
#16
Tomoya Kamide, Jan-Karl Burkhardt, Halima Tabani, Michael Safaee, Michael T Lawton
BACKGROUND: Although most posterior communicating artery (PCoA) aneurysms can be clipped easily with excellent results, some require anterior clinoidectomy for safe and complete clipping. OBJECTIVE: To review our microsurgical series of ruptured PCoA aneurysms and identify the preoperative predictors for anterior clinoidectomy during microsurgical clipping for PCoA aneurysms METHODS: Results from microsurgical clipping of 104 patients with ruptured PCoA aneurysms were retrospectively reviewed...
October 11, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/29031522/video-based-surgical-learning-improving-trainee-education-and-preparation-for-surgery
#17
Paulo Mota, Nuno Carvalho, Emanuel Carvalho-Dias, Manuel João Costa, Jorge Correia-Pinto, Estevão Lima
BACKGROUND: Since the end of the XIX century, teaching of surgery has remained practically unaltered until now. With the dawn of video-assisted laparoscopy, surgery has faced new technical and learning challenges. Due to technological advances, from Internet access to portable electronic devices, the use of online resources is part of the educational armamentarium. In this respect, videos have already proven to be effective and useful, however the best way to benefit from these tools is still not clearly defined...
October 11, 2017: Journal of Surgical Education
https://www.readbyqxmd.com/read/29031174/dynamic-right-ventricular-outflow-obstruction-a-rare-cause-of-hypotension-during-anestesia-induction
#18
Maria Enrica Antoniucci, Christian Colizzi, Gabriella Arlotta, Maria Calabrese, Michele Corrado, Sergio Guarneri, Lorenzo Martinelli, Andrea Scapigliati, Roberto Zamparelli, Franco Cavaliere
INTRODUCTION: Dynamic obstruction of right ventricle outflow tract (RVOTO) is a rare condition that may acutely cause severe heart failure. It has been reported in some hypertrophic cardiomyopathies, after lung transplantation, and in some cases of hemodynamic instability after cardiopulmonary bypass. PRESENTATION OF CASE: We report the case of a 71-year-old man who developed severe hypotension during the induction of general anesthesia for surgical coronary revascularization...
October 5, 2017: International Journal of Surgery Case Reports
https://www.readbyqxmd.com/read/29030926/sternal-malformations-and-anesthetic-management
#19
REVIEW
John H Nichols, Viviane G Nasr
Shamberger and Welch classify sternal malformations into four types: thoracic ectopia cordis, cervical ectopia cordis, thoraco-abdominal ectopia cordis, and cleft sternum. Cleft sternum is the most common subset, with a reported incidence of 1 in 50,000 to 100,000 live births, representing 0.15% of all anterior chest wall malformations. Acostello et al further classify cleft sternum into complete or partial (superior, medium, inferior) with a simple superior partial cleft sternum being by far the most common with an orthotopic heart, intact pericardium, and normal skin coverage...
November 2017: Paediatric Anaesthesia
https://www.readbyqxmd.com/read/29030850/functional-analysis-of-micrornas-in-neurogenesis-during-mouse-cortical-development
#20
Wei Zhang, Xiaoxia Zeng, Li Zeng
The advantage of using in utero electroporation is that it can study the gene function during neurodevelopment in vivo. Using functional analysis of a microRNA (miRNA) gene as an example, this protocol describes a set of techniques that are crucial for the success of neurogenesis studies, including mice time mating, plasmid preparation, utero electroporation following miRNA injection into mice embryonic brain ventricle, labeling of proliferating cells with EDU (ethynyldeoxyuridine), cryosectioning, immunofluorescence staining, and confocal microscopic analysis...
2018: Methods in Molecular Biology
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