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https://www.readbyqxmd.com/read/28719779/cement-casting-to-optimize-reconstruction-of-chronic-osteochondral-lesions-of-the-talus
#1
Jorge Pulgar, Mario Escudero, Giovanni Carcuro, Adam Schiff, Manuel Pellegrini
Few surgical techniques have been described for reconstruction in massive osteochondral lesions of the talus, and there is limited evidence of techniques for accurately reproducing native talar anatomy with bone auto/allograft techniques. In this article, we present a novel technique, which is highly reproducible, using bone cement to restore the congruence and anatomy of the ankle joint. LEVELS OF EVIDENCE: Level V: Technical tip.
August 2017: Foot & Ankle Specialist
https://www.readbyqxmd.com/read/28719214/tunable-liquid-resistant-tip-enhanced-raman-spectroscopy-probes-towards-label-free-nano-resolved-imaging-of-biological-systems
#2
Jacob D Scherger, Mark David Foster
Tip enhanced Raman spectroscopy (TERS) has been established as a powerful, non-invasive technique for chemical identification at the nano-scale. However, difficulties, including the degradation of probes, limit its use in liquid systems. Here TERS probes for studies in aqueous environments have been demonstrated using Titanium Nitride coatings with an alumina protective layer. The probes show enhancement in signal intensity as high as 380% in liquid measurements and the probe resonance can be tuned by varying deposition conditions to optimize performance for different laser sources and types of samples...
July 18, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28719211/real-space-demonstration-of-induced-crystalline-3d-nanostructuration-of-organic-layers
#3
Markos Paradinas, Ana Pérez-Rodríguez, Esther Barrena, Carmen Ocal
The controlled 3D nanostructuration of molecular layers of the semiconducting molecules C22H14 (pentacene) and N, N'-dioctyl-3, 4, 9, 10-perylenetetracarboxylicdiimide (PTCDI-C8) is addressed. A tip-assisted method based using atomic force microscopy (AFM) is developed for removing the organic material and relocating it in up to five layers thick nanostructures. Moreover, unconventional molecular scale imaging combining diverse friction force microscopy modes reveals the stacking sequence of the piled layers...
July 18, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28719182/atomic-white-out-enabling-atomic-circuitry-through-mechanically-induced-bonding-of-single-hydrogen-atoms-to-a-silicon-surface
#4
Taleana Rochelle Huff, Hatem Labidi, Mohammad Rashidi, Mohammad Koleini, Roshan Achal, Mark Henry Salomons, Robert A Wolkow
We report the mechanically induced formation of a silicon-hydrogen covalent bond and its application in engineering nanoelectronic devices. We show that using the tip of a non-contact atomic force microscope (NC-AFM), a single hydrogen atom could be vertically manipulated. When applying a localized electronic excitation, a single hydrogen atom is desorbed from the hydrogen passivated surface and can be transferred to the tip apex as evidenced from a unique signature in frequency shift curves. In the absence of tunnel electrons and electric field in the scanning probe microscope junction at 0 V, the hydrogen atom at the tip apex is brought very close to a silicon dangling bond, inducing the mechanical formation of a silicon-hydrogen covalent bond and the passivation of the dangling bond...
July 18, 2017: ACS Nano
https://www.readbyqxmd.com/read/28718454/high-aspect-ratio-sharp-nanotip-for-nanocantilever-integration-at-cmos-compatible-temperature
#5
P Wang, A Michael, C Y Kwok
In this paper, we demonstrate a novel low temperature nanofabrication approach that enables the formation of ultra-sharp high aspect ratio (HAR) and high density nanotip structures and their integration onto nanoscale cantilever beams. The nanotip structure consists of a nanoscale thermally evaporated Cr Spindt tip on top of an amorphous silicon rod. An apex radius of the tip, as small as 2.5 nm, has been achieved, and is significantly smaller than any other Spindt tips reported so far. 100 nm wide tips with aspect ratio of more than 50 and tip density of more than 5 × 10(9) tips cm(-2) have been fabricated...
August 11, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28718309/intra-articular-fracture-of-the-base-of-the-first-metacarpal-bone-treatment-through-a-volar-approach
#6
Violeta Levy, Marcelo Mazzola, Martín Gonzalez
BACKGROUND: Management of Bennett fractures has been controversial. Early reports supported closed reduction and casting with or without percutaneous pinning. Later, open reduction and internal fixation was advocated. The purpose of this article is to assess the surgical treatment using a direct volar approach. METHODS: Between March 2008 and December 2014, 21 patients with intra-articular first metacarpal fractures displaced more than 1 mm were operated on using a direct volar approach...
January 1, 2017: Hand: Official Journal of the American Association for Hand Surgery
https://www.readbyqxmd.com/read/28717707/misdiagnosis-of-cancer-as-tuberculosis-in-low-to-middle-income-countries-a-tip-of-the-iceberg
#7
Abdul Hannan
No abstract text is available yet for this article.
August 2016: Journal of Global Oncology
https://www.readbyqxmd.com/read/28717634/molar-distalization-with-2k-appliance-one-year-follow-up
#8
Tulika Tripathi, Priyank Rai, Navneet Singh
Correction of class II molars in growing patients with acceptable facial profile can be performed by distalization of maxillary first molars. However, in patients where compliance is difficult intraoral means of molar distalization is required. This case report describes the use and effectiveness of a novel 2K appliance in an 11-year-old female having an orthognathic profile, skeletal Class I relation, and Angle's Class II division 1 malocclusion with crowding of 8 mm and 3 mm in the maxillary and mandibular arches, respectively...
July 2017: Journal of Orthodontic Science
https://www.readbyqxmd.com/read/28717570/chip-on-the-tip-compact-flexible-endoscopic-epifluorescence-video-microscope-for-in-vivo-imaging-in-medicine-and-biomedical-research
#9
Gregor Matz, Bernhard Messerschmidt, Werner Göbel, Severin Filser, Christian S Betz, Matthias Kirsch, Ortrud Uckermann, Marcel Kunze, Sven Flämig, André Ehrhardt, Klaus-Martin Irion, Mareike Haack, Mario M Dorostkar, Jochen Herms, Herbert Gross
We demonstrate a 60 mg light video-endomicroscope with a cylindrical shape of the rigid tip of only 1.6 mm diameter and 6.7 mm length. A novel implementation method of the illumination unit in the endomicroscope is presented. It allows for the illumination of the biological sample with fiber-coupled LED light at 455 nm and the imaging of the red-shifted fluorescence light above 500 nm in epi-direction. A large numerical aperture of 0.7 leads to a sub-cellular resolution and yields to high-contrast images within a field of view of 160 μm...
July 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28717555/adaptive-nonparametric-kinematic-modeling-of-concentric-tube-robots
#10
Georgios Fagogenis, Christos Bergeles, Pierre E Dupont
Concentric tube robots comprise telescopic precurved elastic tubes. The robot's tip and shape are controlled via relative tube motions, i.e. tube rotations and translations. Non-linear interactions between the tubes, e.g. friction and torsion, as well as uncertainty in the physical properties of the tubes themselves, e.g. the Young's modulus, curvature, or stiffness, hinder accurate kinematic modelling. In this paper, we present a machine-learning-based methodology for kinematic modelling of concentric tube robots and in situ model adaptation...
October 2016: Proceedings of the ... IEEE/RSJ International Conference on Intelligent Robots and Systems
https://www.readbyqxmd.com/read/28716699/bridge-to-success-a-better-method-of-cryoablation-for-atrioventricular-nodal-reentrant-tachycardia-in-children
#11
Charitha D Reddy, Scott R Ceresnak, Kara S Motonaga, Kishor Avasarala, Christine Feller, Anthony Trela, Debra Hanisch, Anne M Dubin
BACKGROUND: Cryoablation for AVNRT is associated with higher recurrence rates than radiofrequency ablation(RF). Junctional tachycardia marks procedural success with RF, but no such indicator exists for cryoablation. OBJECTIVE: To determine the impact of voltage mapping plus longer ablation lesions on mid-term success of cryoablation for children with AVNRT. METHODS: A single-center retrospective analysis was performed of pediatric patients with AVNRT who underwent cryoablation from 2011-2015...
July 14, 2017: Heart Rhythm: the Official Journal of the Heart Rhythm Society
https://www.readbyqxmd.com/read/28716177/septal-extension-graft-in-closed-revision-rhinoplasty-a-simplified-technique
#12
Alberto Scattolin, Pier-Francesco Galzignato, Fabrizio Longari, Luca D'Ascanio
BACKGROUND: The septal extension graft (SEG) is widely used in secondary rhinoplasty for correction of a short nose with loss of tip support and projection, columellar retraction, and hyper-rotated tip secondary to an overresection of the caudal septum. Although SEG precise fixation can be relatively easy in the external approach, the difficulties of SEG placement in "closed" rhinoplasty can be discouraging. OBJECTIVE: To describe an easy surgical technique for SEG placement in endonasal revision rhinoplasty and to analyze the aesthetic results of the procedure...
July 1, 2017: American Journal of Rhinology & Allergy
https://www.readbyqxmd.com/read/28716035/systematic-evaluation-of-characteristics-of-the-membrane-based-fed-batch-shake-flask
#13
P Philip, K Meier, D Kern, J Goldmanns, F Stockmeier, C Bähr, J Büchs
BACKGROUND: The initial part of process development involves extensive screening programs to identify optimal biological systems and cultivation conditions. For a successful scale-up, the operation mode on screening and production scale must be as close as possible. To enable screening under fed-batch conditions, the membrane-based fed-batch shake flask was developed. It is a shake flask mounted with a central feed reservoir with an integrated rotating membrane tip for a controlled substrate release...
July 17, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28715856/nitrogen-deposition-changes-ectomycorrhizal-communities-in-swiss-beech-forests
#14
L C de Witte, N P Rosenstock, S van der Linde, S Braun
Atmospheric pollution has implications for the health and diversity of temperate forests covering large parts of central Europe. Long-term elevated anthropogenic deposition of nitrogen (N) is driving forest ecosystems from the limitation by N to other nutrients and is found to affect tree health and ectomycorrhizal fungi (EMF), which most trees depend on for nutrient uptake. However, the consequence of EMF community changes for trees remains unclear. Therefore, we investigated changes in EMF communities on root tips and in soil of beech forests along a N deposition gradient ranging between 16 and 33kgNha(-1)a(-1), where high N deposition was found to negatively affect tree growth and nutrient levels...
July 11, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28715647/the-role-of-netrin-1-in-angiogenesis-and-diabetic-retinopathy-a-promising-therapeutic-strategy
#15
Wenyi Wu, Hetian Lei, Junhui Shen, Luosheng Tang
Netrin-1 is an axon guidance cue and is necessary for neural and vascular development. It is involved in regulating axon guidance for attraction or repulsion, and it has a dual function in endothelial tip cell migration during angiogenesis. Netrin-1 has been shown to play an important role in angiogenesis, cancer progression, and inflammatory disease. Here we review the role of netrin-1 in retinal and angiogenesis development and the associated signaling pathways in diabetic retinopathy. The currently available data suggest that netrin-1 is a promising target for the development of anti-angiogenesis drugs...
May 2017: Discovery Medicine
https://www.readbyqxmd.com/read/28715542/simultaneous-analysis-of-elastic-and-non-specific-adhesive-properties-of-thin-sample-and-biological-cell-considering-bottom-substrate-effect
#16
Viswanath Managuli, Sitikantha Roy
A new asymptotically correct contact model has been developed for conical tip based AFM nanoindentation. This new model provides both elastic and non-specific adhesion properties of cells and soft gels by taking sample thickness at the point of indentation and its depth of indentation into consideration. The bottom substrate effect is the most common source of error in the study of "AFM force maps" of the cellular sample. The present model incorporates an asymptotically correct correction term as a function of depth of indentation to eliminate the substrate effect in the analysis...
July 17, 2017: Journal of Biomechanical Engineering
https://www.readbyqxmd.com/read/28715393/generation-and-long-term-maintenance-of-nerve-free-hydra
#17
Cassidy M Tran, Sharon Fu, Trevor Rowe, Eva-Maria S Collins
The interstitial cell lineage of Hydra includes multipotent stem cells, and their derivatives: gland cells, nematocytes, germ cells, and nerve cells. The interstitial cells can be eliminated through two consecutive treatments with colchicine, a plant-derived toxin that kills dividing cells, thus erasing the potential for renewal of the differentiated cells that are derived from the interstitial stem cells. This allows for the generation of Hydra that lack nerve cells. A nerve-free polyp cannot open its mouth to feed, egest, or regulate osmotic pressure...
July 7, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28715390/mammalian-cell-encapsulation-in-alginate-beads-using-a-simple-stirred-vessel
#18
Corinne A Hoesli, Roger L J Kiang, Kamini Raghuram, René G Pedroza, Karen E Markwick, Antonio M R Colantuoni, James M Piret
Cell encapsulation in alginate beads has been used for immobilized cell culture in vitro as well as for immunoisolation in vivo. Pancreatic islet encapsulation has been studied extensively as a means to increase islet survival in allogeneic or xenogeneic transplants. Alginate encapsulation is commonly achieved by nozzle extrusion and external gelation. Using this method, cell-containing alginate droplets formed at the tip of nozzles fall into a solution containing divalent cations that cause ionotropic alginate gelation as they diffuse into the droplets...
June 29, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28714969/endothelial-notch-signalling-limits-angiogenesis-via-control-of-artery-formation
#19
Sana S Hasan, Roman Tsaryk, Martin Lange, Laura Wisniewski, John C Moore, Nathan D Lawson, Karolina Wojciechowska, Hans Schnittler, Arndt F Siekmann
Angiogenic sprouting needs to be tightly controlled. It has been suggested that the Notch ligand dll4 expressed in leading tip cells restricts angiogenesis by activating Notch signalling in trailing stalk cells. Here, we show using live imaging in zebrafish that activation of Notch signalling is rather required in tip cells. Notch activation initially triggers expression of the chemokine receptor cxcr4a. This allows for proper tip cell migration and connection to the pre-existing arterial circulation, ultimately establishing functional arterial-venous blood flow patterns...
July 17, 2017: Nature Cell Biology
https://www.readbyqxmd.com/read/28714968/dll4-and-notch-signalling-couples-sprouting-angiogenesis-and-artery-formation
#20
Mara E Pitulescu, Inga Schmidt, Benedetto Daniele Giaimo, Tobiah Antoine, Frank Berkenfeld, Francesca Ferrante, Hongryeol Park, Manuel Ehling, Daniel Biljes, Susana F Rocha, Urs H Langen, Martin Stehling, Takashi Nagasawa, Napoleone Ferrara, Tilman Borggrefe, Ralf H Adams
Endothelial sprouting and proliferation are tightly coordinated processes mediating the formation of new blood vessels during physiological and pathological angiogenesis. Endothelial tip cells lead sprouts and are thought to suppress tip-like behaviour in adjacent stalk endothelial cells by activating Notch. Here, we show with genetic experiments in postnatal mice that the level of active Notch signalling is more important than the direct Dll4-mediated cell-cell communication between endothelial cells. We identify endothelial expression of VEGF-A and of the chemokine receptor CXCR4 as key processes controlling Notch-dependent vessel growth...
July 17, 2017: Nature Cell Biology
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