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Muscle energy technique

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https://www.readbyqxmd.com/read/28721834/the-interaction-between-nutrition-and-exercise-for-promoting-health-and-performance
#1
Oliver C Witard, Derek Ball
The theme of The Nutrition Society Spring Conference 2017 was on the interaction between nutrition and exercise for promoting healthy ageing, maintaining cognitive function and improving the metabolic health of the population. The importance of this theme is highlighted by the public health issues surrounding obesity, diabetes and the age-related loss of skeletal muscle mass (sarcopenia). The opening symposium provided a historical perspective of both invasive and non-invasive methodologies for measuring exercise energetics and energy balance...
July 19, 2017: Proceedings of the Nutrition Society
https://www.readbyqxmd.com/read/28683062/use-of-luciferase-probes-to-measure-atp-in-living-cells-and-animals
#2
Giampaolo Morciano, Alba Clara Sarti, Saverio Marchi, Sonia Missiroli, Simonetta Falzoni, Lizzia Raffaghello, Vito Pistoia, Carlotta Giorgi, Francesco Di Virgilio, Paolo Pinton
ATP, the energy exchange factor that connects anabolism and catabolism, is required for major reactions and processes that occur in living cells, such as muscle contraction, phosphorylation and active transport. ATP is also the key molecule in extracellular purinergic signaling mechanisms, with an established crucial role in inflammation and several additional disease conditions. Here, we describe detailed protocols to measure the ATP concentration in isolated living cells and animals using luminescence techniques based on targeted luciferase probes...
August 2017: Nature Protocols
https://www.readbyqxmd.com/read/28620318/extraction-protocols-for-individual-zebrafish-s-ventricle-myosin-and-skeletal-muscle-actin-for-in-vitro-motility-assays
#3
Lisa-Mareike Scheid, Cornelia Weber, Nasrin Bopp, Matias Mosqueira, Rainer H A Fink
The in vitro motility assay (IVMA) is a technique that enables the measurement of the interaction between actin and myosin providing a relatively simple model to understand the mechanical muscle function. For actin-myosin IVMA, myosin is immobilized in a measurement chamber, where it converts chemical energy provided by ATP hydrolysis into mechanical energy. The result is the movement of fluorescently labeled actin filaments that can be recorded microscopically and analyzed quantitatively. Resulting sliding speeds and patterns help to characterize the underlying actin-myosin interaction that can be affected by different factors such as mutations or active compounds...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28611675/cardiac-autonomic-responses-during-exercise-and-post-exercise-recovery-using-heart-rate-variability-and-systolic-time-intervals-a-review
#4
REVIEW
Scott Michael, Kenneth S Graham, Glen M Davis
Cardiac parasympathetic activity may be non-invasively investigated using heart rate variability (HRV), although HRV is not widely accepted to reflect sympathetic activity. Instead, cardiac sympathetic activity may be investigated using systolic time intervals (STI), such as the pre-ejection period. Although these autonomic indices are typically measured during rest, the "reactivity hypothesis" suggests that investigating responses to a stressor (e.g., exercise) may be a valuable monitoring approach in clinical and high-performance settings...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28559721/non-pharmacological-therapies-for-primary-open-angle-glaucoma-a-quasi-experimental-pilot-study
#5
Rahul Pandey, Asir John Samuel, Vencita Priyanka Aranha, Anamika Pandey, Kanimozhi Narkeesh
Purpose: One of the major causes of blindness is Primary open angle glaucoma (POAG) and it has only surgical treatment and lifelong use of medication. Hence many side effects arise. To overcome this, the drugless approach is in practice but the importance of Muscle Energy Technique (MET) and Myofacial (MFR) Release is not explored. Hence, our objective was to determine the effectiveness of MET and MFR on POAG. Methods: A total of 12 patients with POAG were recruited from the tertiary care teaching hospital through criteria based convenience sampling for the study...
April 2017: Saudi Journal of Ophthalmology: Official Journal of the Saudi Ophthalmological Society
https://www.readbyqxmd.com/read/28555757/using-urinary-parameters-to-estimate-seasonal-variation-in-the-physical-condition-of-female-white-faced-capuchin-monkeys-cebus-capucinus-imitator
#6
Mackenzie L Bergstrom, Melissa Emery Thompson, Amanda D Melin, Linda M Fedigan
OBJECTIVES: The physical condition of females depends on access to resources, which vary over space and time. Assessing variation in physical condition can help identify factors affecting reproductive success, but noninvasive measurement is difficult in wild animals. Creatinine concentration relative to the specific gravity (i.e., density) of urine has promise for noninvasively quantifying the relative muscle mass (RMM) of wild primates. We verified the relationship between these urinary parameters for wild white-faced capuchin monkeys, and assessed temporal changes in the RMM of females across groups and between periods of high and low resource abundance...
May 27, 2017: American Journal of Physical Anthropology
https://www.readbyqxmd.com/read/28503503/measurement-methods-for-physical-activity-and-energy-expenditure-a-review
#7
REVIEW
Didace Ndahimana, Eun-Kyung Kim
Physical activity is defined as any bodily movement produced by skeletal muscles that results in energy expenditure. The benefits of physical activity for health maintenance have been well documented, especially in the prevention and management of chronic diseases. Therefore, accurate measurement of physical activity and energy expenditure is essential both for epidemiological studies and in the clinical context. Given the large number of available methods, it is important to have an understanding of each, especially when one needs to choose a technique to use...
April 2017: Clinical Nutrition Research
https://www.readbyqxmd.com/read/28494371/improving-surface-emg-burst-detection-in-infrahyoid-muscles-during-swallowing-using-digital-filters-and-discrete-wavelet-analysis
#8
Sebastian Restrepo-Agudelo, Sebastian Roldan-Vasco, Lina Ramirez-Arbelaez, Santiago Cadavid-Arboleda, Estefania Perez-Giraldo, Andres Orozco-Duque
The visual inspection is a widely used method for evaluating the surface electromyographic signal (sEMG) during deglutition, a process highly dependent of the examiners expertise. It is desirable to have a less subjective and automated technique to improve the onset detection in swallowing related muscles, which have a low signal-to-noise ratio. In this work, we acquired sEMG measured in infrahyoid muscles with high baseline noise of ten healthy adults during water swallowing tasks. Two methods were applied to find the combination of cutoff frequencies that achieve the most accurate onset detection: discrete wavelet decomposition based method and fixed steps variations of low and high cutoff frequencies of a digital bandpass filter...
August 2017: Journal of Electromyography and Kinesiology
https://www.readbyqxmd.com/read/28489897/analysis-of-statistical-and-standard-algorithms-for-detecting-muscle-onset-with-surface-electromyography
#9
Matthew S Tenan, Andrew J Tweedell, Courtney A Haynes
The timing of muscle activity is a commonly applied analytic method to understand how the nervous system controls movement. This study systematically evaluates six classes of standard and statistical algorithms to determine muscle onset in both experimental surface electromyography (EMG) and simulated EMG with a known onset time. Eighteen participants had EMG collected from the biceps brachii and vastus lateralis while performing a biceps curl or knee extension, respectively. Three established methods and three statistical methods for EMG onset were evaluated...
2017: PloS One
https://www.readbyqxmd.com/read/28481993/inherited-eye-related-disorders-due-to-mitochondrial-dysfunction
#10
Patrick Yu-Wai-Man, Nancy J Newman
Genetic disorders due to mitochondrial dysfunction are not uncommon and the majority of these patients will have eye-related manifestations, including visual loss from optic nerve and retinal disease, visual field loss from retrochiasmal visual pathway damage, and ptosis and ocular dysmotility from extraocular muscle involvement. Defects in both the nuclear and mitochondrial genomes cause mitochondrial dysfunction via several mechanisms, including impaired mitochondrial energy production, oxidative stress, mitochondrial DNA instability, abnormalities in the regulation of mitochondrial dynamics and mitochondrial quality control, and disturbed cellular interorganellar communication...
May 8, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/28455271/evaluation-of-ablation-catheter-technology-comparison-between-thigh-preparation-model-and-an-in%C3%A2-vivo-beating-heart
#11
Eran Leshem, Cory M Tschabrunn, Fernando M Contreras-Valdes, Israel Zilberman, Elad Anter
BACKGROUND: An in vivo animal thigh model is the standard technique for evaluation of ablation catheter technologies, including efficacy and safety of ablation. However, the biophysics of ablation in a thigh model may not be similar to a beating heart. OBJECTIVE: The purpose of this study was to compare efficacy and safety of ablation between a thigh preparation model and a beating heart. METHODS: In 7 swine, radiofrequency ablation using a 3...
April 26, 2017: Heart Rhythm: the Official Journal of the Heart Rhythm Society
https://www.readbyqxmd.com/read/28437606/transfer-study-of-silver-nanoparticles-in-poultry-production
#12
Federica Gallocchio, Giancarlo Biancotto, Veronica Cibin, Carmen Losasso, Simone Belluco, Ruud Peters, Greet van Bemmel, Claudia Cascio, Stefan Weigel, Peter Tromp, Federica Gobbo, Salvatore Catania, Antonia Ricci
Silver nanoparticles (AgNPs) are of interest due to their antimicrobial activity and are seen as potential candidates to replace antibiotics in animal husbandry. A few studies have focused on this new application, but they lack any considerations about residual accumulation of AgNPs in edible animal tissues and animal products. In this research, a 22 day in vivo study was carried out by oral administration of 20 nm spherical PVP coated AgNPs to hens. Six doses of approximately 1 mg kg(-1) of AgNPs-PVP each were administered to animals throughout the experimentation...
May 10, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28412225/enhancing-tissue-permeability-with-mri-guided-preclinical-focused-ultrasound-system-in-rabbit-muscle-from-normal-tissue-to-vx2-tumor
#13
Yao Sun, Xiaobing Xiong, Darpan Pandya, Youngkyoo Jung, Akiva Mintz, Satoru Hayasaka, Thaddeus J Wadas, King C P Li
High Intensity Focused Ultrasound (HIFU) is an emerging noninvasive, nonionizing physical energy based modality to ablate solid tumors with high power, or increase local permeability in tissues/tumors in pulsed mode with relatively low power. Compared with traditional ablative HIFU, nondestructive pulsed HIFU (pHIFU) is present in the majority of novel applications recently developed for enhancing the delivery of drugs and genes. Previous studies have demonstrated the capability of pHIFU to change tissue local permeability for enhanced drug delivery in both mouse tumors and mouse muscle...
June 28, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28395773/improving-data-quality-and-preserving-hcd-generated-reporter-ions-with-ethcd-for-isobaric-tag-based-quantitative-proteomics-and-proteome-wide-ptm-studies
#14
Qing Yu, Xudong Shi, Yu Feng, K Craig Kent, Lingjun Li
Mass spectrometry (MS)-based isobaric labeling has undergone rapid development in recent years due to its capability for high throughput quantitation. Apart from its originally designed use with collision-induced dissociation (CID) and higher-energy collisional dissociation (HCD), isobaric tagging technique could also work with electron-transfer dissociation (ETD), which provides complementarity to CID and is preferred in sequencing peptides with post-translational modifications (PTMs). However, ETD suffers from long reaction time, reduced duty cycle and bias against peptides with lower charge states...
May 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28362420/isolation-of-intact-mitochondria-from-skeletal-muscle-by-differential-centrifugation-for-high-resolution-respirometry-measurements
#15
Siamak Djafarzadeh, Stephan Mathias Jakob
Mitochondria are involved in cellular energy metabolism and use oxygen to produce energy in the form of adenosine triphosphate (ATP). Differential centrifugation at low- and high-speed is commonly used to isolate mitochondria from tissues and cultured cells. Crude mitochondrial fractions obtained by differential centrifugation are used for respirometry measurements. The differential centrifugation technique is based on the separation of organelles according to their size and sedimentation velocity. The isolation of mitochondria is performed immediately after tissue harvesting...
March 8, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28338413/human-tissue-engineered-skeletal-muscle-myobundles-to-measure-oxygen-uptake-and-assess-mitochondrial-toxicity
#16
Brittany N J Davis, Jeffrey W Santoso, Michaela J Walker, Cindy S Cheng, Timothy R Koves, William E Kraus, George A Truskey
Mitochondrial dysfunction is responsible for the toxicity of a number of drugs. Current isolated mitochondria or cellular monoculture mitochondrial respiration measurement systems lack physiological relevance. Using a tissue engineering rather than cell- or mitochondria-based approach enables a more physiologically relevant detection of drug-induced mitochondrial impairment. To probe oxygen consumption and mitochondrial health, we assayed the bioenergetic profile of engineered three-dimensional human skeletal muscle myobundles derived from primary myoblasts...
April 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28325000/mechanomyography-signals-in-spastic-muscle-and-the-correlation-with-the-modified-ashworth-scale
#17
E L Santos, M C Santos, E Krueger, G N Nogueira-Neto, P Nohama
The modified Ashworth scale (MAS) is the most widely used measurement technique to assess levels of spasticity. In MAS, the evaluator graduates spasticity considering his/her subjective analysis of the muscular endurance during passive stretching. Therefore, it is a subjective scale. Mechanomyography (MMG) allows registering the vibrations generated by muscle contraction and stretching events that propagate through the tissue until the surface of the skin. With this in mind, this study aimed to investigate possible correlations between MMG signal and muscle spasticity levels determined by MAS...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28293194/quantification-of-internal-stress-strain-fields-in-human-tendon-unraveling-the-mechanisms-that-underlie-regional-tendon-adaptations-and-mal-adaptations-to-mechanical-loading-and-the-effectiveness-of-therapeutic-eccentric-exercise
#18
REVIEW
Constantinos N Maganaris, Panagiotis Chatzistergos, Neil D Reeves, Marco V Narici
By virtue of their anatomical location between muscles and bones, tendons make it possible to transform contractile force to joint rotation and locomotion. However, tendons do not behave as rigid links, but exhibit viscoelastic tensile properties, thereby affecting the length and contractile force in the in-series muscle, but also storing and releasing elastic stain energy as some tendons are stretched and recoiled in a cyclic manner during locomotion. In the late 90s, advancements were made in the application of ultrasound scanning that allowed quantifying the tensile deformability and mechanical properties of human tendons in vivo...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28272024/multidomain-proteins-under-force
#19
Jessica Valle-Orero, Jaime Andrés Rivas-Pardo, Ionel Popa
Advancements in single-molecule force spectroscopy techniques such as atomic force microscopy and magnetic tweezers allow investigation of how domain folding under force can play a physiological role. Combining these techniques with protein engineering and HaloTag covalent attachment, we investigate similarities and differences between four model proteins: I10 and I91-two immunoglobulin-like domains from the muscle protein titin, and two α + β fold proteins-ubiquitin and protein L. These proteins show a different mechanical response and have unique extensions under force...
April 28, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28227340/mechanomyography-signals-in-spastic-muscle-and-the-correlation-with-the-modified-ashworth-scale
#20
E L Santos, M C Santos, E Krueger, G N Nogueira-Neto, P Nohama, E L Santos, M C Santos, E Krueger, G N Nogueira-Neto, P Nohama, G N Nogueira-Neto, M C Santos, E Krueger, E L Santos, P Nohama
The modified Ashworth scale (MAS) is the most widely used measurement technique to assess levels of spasticity. In MAS, the evaluator graduates spasticity considering his/her subjective analysis of the muscular endurance during passive stretching. Therefore, it is a subjective scale. Mechanomyography (MMG) allows registering the vibrations generated by muscle contraction and stretching events that propagate through the tissue until the surface of the skin. With this in mind, this study aimed to investigate possible correlations between MMG signal and muscle spasticity levels determined by MAS...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
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