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https://www.readbyqxmd.com/read/28087758/method-for-the-determination-of-carbonyl-compounds-in-e-cigarette-aerosols
#1
Jason W Flora, Celeste T Wilkinson, James W Wilkinson, Peter J Lipowicz, James A Skapars, Adam Anderson, John H Miller
Low levels of thermal degradation products such as carbonyls (formaldehyde, acetaldehyde, acrolein, crotonaldehyde) have been reported in e-cigarette aerosols. The collection and analysis of e-cigarette aerosol carbonyls are often adapted from methods developed for tobacco cigarette smoke. These methodologies are often not sensitive enough to detect low carbonyl levels in e-cigarette aerosols. One objective of this work was to develop and validate a rapid, selective and sensitive ultra-performance liquid chromatography with mass spectrometry method optimized for analysis of carbonyls in e-cigarette aerosols...
February 2017: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/28087244/practice-makes-perfect-correlations-between-prior-experience-in-high-level-athletics-and-robotic-surgical-performance-do-not-persist-after-task-repetition
#2
Kevin Shee, Fady M Ghali, Elias S Hyams
OBJECTIVE: Robotic surgical skill development is central to training in urology as well as in other surgical disciplines. Here, we describe a pilot study assessing the relationships between robotic surgery simulator performance and 3 categories of activities, namely, videogames, musical instruments, and athletics. DESIGN: A questionnaire was administered to preclinical medical students for general demographic information and prior experiences in surgery, videogames, musical instruments, and athletics...
January 10, 2017: Journal of Surgical Education
https://www.readbyqxmd.com/read/28086746/mviaeval-a-web-tool-for-comprehensively-evaluating-the-performance-of-a-new-missing-value-imputation-algorithm
#3
Wei-Sheng Wu, Meng-Jhun Jhou
BACKGROUND: Missing value imputation is important for microarray data analyses because microarray data with missing values would significantly degrade the performance of the downstream analyses. Although many microarray missing value imputation algorithms have been developed, an objective and comprehensive performance comparison framework is still lacking. To solve this problem, we previously proposed a framework which can perform a comprehensive performance comparison of different existing algorithms...
January 13, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28081502/quantitative-determinations-using-portable-raman-spectroscopy
#4
Chelliah V Navin, Chaitanya Tondepu, Roxana Toth, Latevi S Lawson, Jason D Rodriguez
A portable Raman spectrometer was used to develop chemometric models to determine percent (%) drug release and potency for 500mg ciprofloxacin HCl tablets. Parallel dissolution and chromatographic experiments were conducted alongside Raman experiments to assess and compare the performance and capabilities of portable Raman instruments in determining critical drug attributes. All batches tested passed the 30min dissolution specification and the Raman model for drug release was able to essentially reproduce the dissolution profiles obtained by ultraviolet spectroscopy at 276nm for all five batches of the 500mg ciprofloxacin tablets...
December 30, 2016: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28081498/enhancing-analysis-throughput-sensitivity-and-specificity-in-lc-esi-ms-ms-assay-of-plasma-25-hydroxyvitamin-d3-by-derivatization-with-triplex-4-4-dimethylaminophenyl-1-2-4-triazoline-3-5-dione-daptad-isotopologues
#5
Shoujiro Ogawa, Hiroki Kittaka, Akiho Nakata, Kenji Komatsu, Takahiro Sugiura, Mamoru Satoh, Fumio Nomura, Tatsuya Higashi
The plasma/serum concentration of 25-hydroxyvitamin D3 [25(OH)D3] is a diagnostic index for vitamin D deficiency/insufficiency, which is associated with a wide range of diseases, such as rickets, cancer and diabetes. We have reported that the derivatization with 4-(4-dimethylaminophenyl)-1,2,4-triazoline-3,5-dione (DAPTAD) works well in the liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS) assay of the serum/plasma 25(OH)D3 for enhancing the sensitivity and the separation from a potent interfering metabolite, 3-epi-25-hydroxyvitamin D3 [3-epi-25(OH)D3]...
November 17, 2016: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28079215/selective-releasing-affected-lubricant-mechanism-of-a-self-assembled-mos2-mo-s-c-nanoperiod-multilayer-film-sliding-in-diverse-atmospheres
#6
J Xu, T F He, L Q Chai, L Qiao, X Q Zhang, P Wang, W M Liu
A self-assembled MoS2/Mo-S-C multilayer film prepared by r.f. co-sputtering of MoS2 and graphite targets was tribotested in diverse sliding atmospheres, and the lubricant mechanism and its correlations to the selective releasing behavior of non-lubricant component were analyzed in detail. Based on the analysis of composition and structural transformation of transferred materials by Raman and HRTEM characterizations, selective releasing behavior according to the sliding atmospheres were found to be particularly apparent in vacuum and dry inert atmospheres...
January 12, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28079019/an-efficient-targeted-nuclease-strategy-for-high-resolution-mapping-of-dna-binding-sites
#7
Peter J Skene, Steven Henikoff
We describe Cleavage Under Targets and Release Using Nuclease (CUT&RUN), a chromatin profiling strategy in which antibody-targeted controlled cleavage by micrococcal nuclease releases specific protein-DNA complexes into the supernatant for paired-end DNA sequencing. Unlike Chromatin Immunoprecipitation (ChIP), which fragments and solubilizes total chromatin, CUT&RUN is performed in situ, allowing for both quantitative high-resolution chromatin mapping and probing of the local chromatin environment. When applied to yeast and human nuclei, CUT&RUN yielded precise transcription factor profiles while avoiding cross-linking and solubilization issues...
January 12, 2017: ELife
https://www.readbyqxmd.com/read/28077910/the-first-mediterranean-seminar-on-science-writing-editing-and-publishing-sarajevo-december-2-3-2016
#8
REVIEW
Izet Masic, Doncho Donev, Osman Sinanovic, Miro Jakovljevic, Enver Zerem, Dejan B Milosevic, Srecko Gajovic, Armen Yuri Gasparyan, Sekib Sokolovic, Nermin Salkic, Selma Uzunovic, Silva Dobric, Floreta Kurti, Edin Begic, Admir Kurtcehajic
The First Mediterranean Seminar on Science Writing, Editing & Publishing (SWEP 2016) was held in Sarajevo, Bosnia & Herzegovina from 2nd to 3rd December 2016. It was organized by Academy of Medical Sciences of Bosnia and Herzegovina, running concurrent sessions as part of its Annual Meeting titled " "Days of AMNuBiH - Theory and Practice in Science Communication and Scientometrics". Hotel Bosnia in the city centre was the chosen venue. On the first day, nineteen presentations on various issues of science writing and publication ethics were delivered by speakers from Croatia, Serbia, Macedonia, Albania, Bosnia & Herzegovina and the UK (Asim Kurjak, Milivoj Boranić, Doncho Donev, Osman Sinanović, Miro Jakovljević, Enver Zerem, Dejan Milošević, Silva Dobrić, Srećko Gajović, Izet Mašić, Armen Yuri Gasparyan, Šekib Sokolović, Nermin Salkić, Selma Uzunović, Admir Kurtčehajić, Edin Begić and Floreta Kurti)...
December 2016: Acta Informatica Medica: AIM
https://www.readbyqxmd.com/read/28077751/performance-of-health-workers-using-an-electronic-algorithm-for-the-management-of-childhood-illness-in-tanzania-a-pilot-implementation-study
#9
Clotilde Rambaud-Althaus, Amani Shao, Josephine Samaka, Ndeniria Swai, Seneca Perri, Judith Kahama-Maro, Marc Mitchell, Valérie D'Acremont, Blaise Genton
In low-resource settings, where qualified health workers (HWs) are scarce and childhood mortality high, rational antimicrobial prescription for childhood illnesses is a challenge. To assess whether smartphones running guidelines, as compared with paper support, improve consultation process and rational use of medicines for children, a pilot cluster-randomized controlled study was conducted in Tanzania. Nine primary health-care facilities (HFs) were randomized into three arms: 1) paper algorithm, 2) electronic algorithm on a smartphone, and 3) control...
January 11, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28076690/effect-of-intermittent-aeration-strategies-on-treatment-performance-and-microbial-community-of-an-ifas-reactor-treating-municipal-wastewater
#10
Nitin Kumar Singh, Akansha Bhatia, Absar Ahmad Kazmi
This study investigated the effect of various intermittent aeration (IA) cycles, characterized by the ratio of non-aeration to aeration time, on organics and nutrient removal, and microbial communities in an integrated fixed film activated sludge (IFAS) reactor treating municipal wastewater. Average effluent BOD, COD, TSS, TN and TP values were noted as 20, 50, 30, 12 and 1.5 mgL(-1), respectively in continuous aeration mode. A total of four operational conditions (run 1, continuous aeration; run 2, 150/30 min aeration on/off time; run 3, 120/60 min aeration on/off time; run 4, 90/60 min aeration on/off time) were investigated in IFAS reactor assessment...
January 11, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28075184/elucidation-of-formulation-and-delivery-device-related-effects-on-in-vitro-performance-of-nasal-spray-with-implication-to-rational-product-specification-identification
#11
Jernej Grmaš, Katarina Stare, Dane Božič, Rade Injac, Rok Dreu
BACKGROUND: The aim of this work is to use an experimental design approach to identify and study influential formulation and delivery device properties, which can be controlled by final product manufacturer, to establish design space, within which desired in vitro performance can be reached. METHODS: Combining three factors, viscosity of suspension, nozzle orifice diameter (OD), and shot weight (SW), at three levels resulted in D-optimal experimental design with 20 runs...
January 11, 2017: Journal of Aerosol Medicine and Pulmonary Drug Delivery
https://www.readbyqxmd.com/read/28073073/development-and-validation-of-a-liquid-chromatography-ms-ms-method-for-simultaneous-quantification-of-tenofovir-and-efavirenz-in-biological-tissues-and-fluids
#12
Luisa Barreiros, Cassilda Cunha-Reis, Eduarda M P Silva, Joana R B Carvalho, José das Neves, Bruno Sarmento, Marcela A Segundo
Millions of people worldwide live with human immunodeficiency virus (HIV) infection thus justifying the continuous search for new prevention and treatment strategies, including topical microbicide products combining antiretroviral drugs (ARVs) such as tenofovir (TFV) and efavirenz (EFV). Therefore, the aim of this work was to develop and validate a high performance liquid chromatography method coupled to triple quadrupole-tandem mass spectrometry (HPLC-MS/MS) for the quantification of TFV and EFV in biological matrices (mouse vaginal tissue, vaginal lavage and blood plasma)...
December 27, 2016: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28072524/common-hits-approach-combining-pharmacophore-modeling-and-molecular-dynamics-simulations
#13
Marcus Wieder, Arthur Garon, Ugo Perricone, Stefan Boresch, Thomas Seidel, Anna Maria Almerico, Thierry Langer
We present a new approach that incorporates flexibility based on extensive MD simulations of protein-ligand complexes into structure based pharmacophore modeling and virtual screening. The approach uses the multiple coordinate sets saved during the MD simulations and generates for each frame a pharmacophore model. Pharmacophore models with the same pharmacophore features are pooled. In this way the high number of pharmacophore models that results from the MD simulation is reduced to only a few hundred representative pharmacophore models...
January 10, 2017: Journal of Chemical Information and Modeling
https://www.readbyqxmd.com/read/28070602/acupuncture-in-posttonsillectomy-pain-a%C3%A2-prospective-double-blinded-randomized-controlled-trial
#14
J Dingemann, B Plewig, I Baumann, P K Plinkert, S Sertel
BACKGROUND: Postoperative swallowing pain is one of the most unpleasant after-effects of tonsillectomy. During recent years, the demand for alternatives to drug-based pain therapy has continued to grow, although the topic has received little research attention until now. MATERIALS AND METHODS: A total of 46 patients were randomized into verum acupuncture, control acupuncture, and drug-based treatment groups. All patients received nonsteroidal antirheumatic drugs (NSAIDs)...
January 9, 2017: HNO
https://www.readbyqxmd.com/read/28070089/development-of-a-one-run-real-time-pcr-detection-system-for-pathogens-associated-with-bovine-respiratory-disease-complex
#15
Mai Kishimoto, Shinobu Tsuchiaka, Sayed Samim Rahpaya, Ayako Hasebe, Keiko Otsu, Satoshi Sugimura, Suguru Kobayashi, Natsumi Komatsu, Makoto Nagai, Tsutomu Omatsu, Yuki Naoi, Kaori Sano, Sachiko Okazaki-Terashima, Mami Oba, Yukie Katayama, Reiichiro Sato, Tetsuo Asai, Tetsuya Mizutani
Bovine respiratory disease complex (BRDC) is frequently found in cattle worldwide. The etiology of BRDC is complicated by infections with multiple pathogens, making identification of the causal pathogen difficult. Here, we developed a detection system by applying TaqMan real-time PCR (Dembo respiratory-PCR) to screen a broad range of microbes associated with BRDC in a single run. We selected 16 bovine respiratory pathogens (bovine viral diarrhea virus, bovine coronavirus, bovine parainfluenza virus 3, bovine respiratory syncytial virus, influenza D virus, bovine rhinitis A virus, bovine rhinitis B virus, bovine herpesvirus 1, bovine adenovirus 3, bovine adenovirus 7, Mannheimia haemolytica, Pasteurella multocida, Histophilus somni, Trueperella pyogenes, Mycoplasma bovis and Ureaplasma diversum) as detection targets and designed novel specific primer-probe sets for nine of them...
January 8, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28069690/development-of-solid-phase-extraction-and-hplc-method-for-simultaneous-estimation-of-ilaprazole-and-glimepiride-in-rat-plasma-application-to-pharmacokinetic-studies
#16
A P Dewani, A S Tripathi, P G Shelke, R L Bakal, D S Mohale, A V Chandewar
A novel, simple and mass spectrometry (MS) compatible high-performance liquid chromatography (HPLC) method is reported for the simultaneous estimation of ilaprazole (ILA) and glimepiride (GLM) in rat plasma. The bio-analytical procedure involves extraction of ILA, GLM and internal standard (IS) from rat plasma with a solid-phase extraction (SPE) process. The chromatographic analysis was performed on Waters-600 system using an isocratic mobile phase comprising methanol:water (80:20 % v/v) with pH of water modified to three using formic acid at a flow rate of 1...
January 8, 2017: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/28068681/hypoxia-and-hyperoxia-affect-serum-angiogenic-regulators-in-t2dm-men-during-cycling
#17
C Brinkmann, A Metten, P Scriba, C V M Tagarakis, P Wahl, J Latsch, K Brixius, W Bloch
Exercise-induced transient increases in pro-angiogenic regulators can promote angiogenesis.This pilot study aims to analyze the potential of exercise to positively affect angiogenic regulators in patients with type 2 diabetes mellitus (T2DM), who often exhibit abnormal angiogenesis, under different environmental conditions. 9 overweight/obese men with uncomplicated T2DM (8 took anti-diabetic drugs) performed submaximal cycling for 40 min in normoxia (≈21 vol%O2), hypoxia (≈14 vol%O2) and during alternating hypoxia/hyperoxia (≈14 vol%O2/≈30 vol%O2, 5-min intervals) (3×3 crossover design)...
January 9, 2017: International Journal of Sports Medicine
https://www.readbyqxmd.com/read/28068561/classifying-the-variability-in-impact-and-active-peak-vertical-ground-reaction-forces-during-running-using-dfa-and-arfima-models
#18
Samantha L Winter, John H Challis
The vertical ground reaction force (VGRF) during rear-foot striking running typically exhibits peaks referred to as the impact peak and the active peak; their timings and magnitudes have been implicated in injury. Identifying the structure of time-series can provide insight into associated control processes. The purpose here was to detect long-range correlations associated with the time from first contact to impact peak (TIP) and active peak (TAP); and the magnitudes of impact (IPM) and active peaks (APM) using a Detrended Fluctuation Analysis, and Auto-Regressive Fractionally Integrated Moving Average models...
January 6, 2017: Human Movement Science
https://www.readbyqxmd.com/read/28068401/advantages-of-synthetic-noise-and-machine-learning-for-analyzing-radioecological-data-sets
#19
Igor Shuryak
The ecological effects of accidental or malicious radioactive contamination are insufficiently understood because of the hazards and difficulties associated with conducting studies in radioactively-polluted areas. Data sets from severely contaminated locations can therefore be small. Moreover, many potentially important factors, such as soil concentrations of toxic chemicals, pH, and temperature, can be correlated with radiation levels and with each other. In such situations, commonly-used statistical techniques like generalized linear models (GLMs) may not be able to provide useful information about how radiation and/or these other variables affect the outcome (e...
2017: PloS One
https://www.readbyqxmd.com/read/28067762/ecologically-valid-carbohydrate-intake-during-soccer-specific-exercise-does-not-affect-running-performance-in-a-fed-state
#20
Mark P Funnell, Nick R Dykes, Elliot J Owen, Stephen A Mears, Ian Rollo, Lewis J James
This study assessed the effect of carbohydrate intake on self-selected soccer-specific running performance. Sixteen male soccer players (age 23 ± 4 years; body mass 76.9 ± 7.2 kg; predicted VO2max = 54.2 ± 2.9 mL∙kg(-1)∙min(-1); soccer experience 13 ± 4 years) completed a progressive multistage fitness test, familiarisation trial and two experimental trials, involving a modified version of the Loughborough Intermittent Shuttle Test (LIST) to simulate a soccer match in a fed state. Subjects completed six 15 min blocks (two halves of 45 min) of intermittent shuttle running, with a 15-min half-time...
January 5, 2017: Nutrients
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