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https://www.readbyqxmd.com/read/28241459/the-effect-of-gender-and-menstrual-phase-on-serum-creatine-kinase-activity-and-muscle-soreness-following-downhill-running
#1
Tanja Oosthuyse, Andrew N Bosch
Serum creatine kinase (CK) activity reflects muscle membrane disruption. Oestrogen has antioxidant and membrane stabilising properties, yet no study has compared the CK and muscle soreness (DOMS) response to unaccustomed exercise between genders when all menstrual phases are represented in women. Fifteen eumenorrhoeic women (early follicular, EF (n = 5); late follicular, LF (n = 5); mid-luteal, ML (n = 5) phase) and six men performed 20 min of downhill running (-10% gradient) at 9 km/h. Serum CK activity and visual analogue scale rating of perceived muscle soreness were measured before, immediately, 24-h, 48-h and 72-h after exercise...
February 23, 2017: Antioxidants (Basel, Switzerland)
https://www.readbyqxmd.com/read/28240715/influence-of-environmental-conditions-on-performance-and-heart-rate-responses-to-the-30-15-incremental-fitness-test-in-rugby-union-athletes
#2
Thomas W Jones, Alex O W Natera, Jacob Jennings, Aiden J Oakley
The purpose of this study was to examine the differences in performance and heart rate responses between a high heat outdoor condition (34.0°C, 64.1% humidity) and a temperate indoor condition (22.0°C, 50.0% humidity) during the 30-15 intermittent fitness test (30-15IFT). Eight highly trained Rugby Union players (28.1 ± 1.5 years, 181.4 ± 8.8 cm, 88.4 ± 13.3kg) completed the 30-15IFT in two different temperature conditions. Dependant variables recorded and analysed included; final running speed of the 30-15IFT, heart rate (HR) at rest (HR rest), maximum HR (Max HR), HR recovery (HRR), average HR (HR ave) and sub-maximal HR corresponding to 25%, 50% and 75% of final test speed (HR 25%, HR 50% and HR 75%) and HR at 13 km·h (HR 13 km·h)...
February 23, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/28240711/a-self-paced-team-sport-match-simulation-results-in-reductions-in-voluntary-activation-and-modifications-to-biological-perceptual-and-performance-measures-at-half-time-and-for-up-to-96-hours-post-match
#3
Paul Tofari, Justin Kemp, Stuart Cormack
Assessing responses to soccer match-play is limited by match variability or unrealistic simulations. To address this, the biological, perceptual, and performance response were assessed using a self-paced, simulated soccer match protocol using a non-motorized treadmill. Twelve male team-sport athletes performed the 90-min simulation. Match activity; quadriceps twitch interpolation [voluntary activation (%VA) and potentiated twitch (POT)]; biochemical markers; strength and power performance; rating of perceived exertion (RPE) and self-report wellness were collected pre-, half-time, post-, and 2, 24, 48, 72 and 96-h post-match...
February 23, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/28240709/changes-in-vertical-and-joint-stiffness-in-runners-with-advancing-age
#4
Douglas W Powell, D S Blaise Williams
BACKGROUND: Age-related changes in the neuromuscular system underlie reduced performance and injury, but may be mitigated through regular physical activity. It was hypothesized that older (OLD) compared to young (YOUNG) adults would exhibit greater vertical and joint stiffness when running at 3.35 m/s. METHODS: Nine YOUNG and ten OLD runners performed over ground running trials while three-dimensional biomechanics were recorded. Ankle and knee joint angles, moments and stiffness values were compared between YOUNG & OLD...
February 23, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/28240238/a-fast-and-efficient-algorithm-for-mining-top-k-nodes-in-complex-networks
#5
Dong Liu, Yun Jing, Jing Zhao, Wenjun Wang, Guojie Song
One of the key problems in social network analysis is influence maximization, which has great significance both in theory and practical applications. Given a complex network and a positive integer k, and asks the k nodes to trigger the largest expected number of the remaining nodes. Many mature algorithms are mainly divided into propagation-based algorithms and topology- based algorithms. The propagation-based algorithms are based on optimization of influence spread process, so the influence spread of them significantly outperforms the topology-based algorithms...
February 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28239998/effects-of-colostrum-serum-on-the-serotonergic-system-in-the-dorsal-raphe-nuclei-of-exercised-rats
#6
Seo JInhee, Kim Tae-Woon, Kim Chang-Ju
Purpose: The central fatigue hypothesis suggests that exhaustion, or the maximum level of exercise, induces excessive stress and increases serotonin concentrations in the brain, which in turn decreases central nervous system (CNS) function and induces fatigue. Our aim was to determine the effects of colostrum serum on the serotonergic system in the dorsal raphe nuclei during exhaustive exercise. Methods: Animals were randomly divided into five groups: control, exercise, exercise and treatment with 50, 100, and 200 mg/kg of colostrum serum...
February 24, 2017: Journal of Exercise Nutrition & Biochemistry
https://www.readbyqxmd.com/read/28239609/application-of-taxonomic-modeling-to-microbiota-data-mining-for-detection-of-helminth-infection-in-global-populations
#7
Mahbaneh Eshaghzadeh Torbati, Makedonka Mitreva, Vanathi Gopalakrishnan
Human microbiome data from genomic sequencing technologies is fast accumulating, giving us insights into bacterial taxa that contribute to health and disease. The predictive modeling of such microbiota count data for the classification of human infection from parasitic worms, such as helminths, can help in the detection and management across global populations. Real-world datasets of microbiome experiments are typically sparse, containing hundreds of measurements for bacterial species, of which only a few are detected in the bio-specimens that are analyzed...
December 2016: Data (Basel)
https://www.readbyqxmd.com/read/28239359/variations-in-hypoxia-impairs-muscle-oxygenation-and-performance-during-simulated-team-sport-running
#8
Alice J Sweeting, François Billaut, Matthew C Varley, Ramón F Rodriguez, William G Hopkins, Robert J Aughey
Purpose: To quantify the effect of acute hypoxia on muscle oxygenation and power during simulated team-sport running. Methods: Seven individuals performed repeated and single sprint efforts, embedded in a simulated team-sport running protocol, on a non-motorized treadmill in normoxia (sea-level), and acute normobaric hypoxia (simulated altitudes of 2,000 and 3,000 m). Mean and peak power was quantified during all sprints and repeated sprints. Mean total work, heart rate, blood oxygen saturation, and quadriceps muscle deoxyhaemoglobin concentration (assessed via near-infrared spectroscopy) were measured over the entire protocol...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28239346/parallel-steps-large-scale-stochastic-spatial-reaction-diffusion-simulation-with-high-performance-computers
#9
Weiliang Chen, Erik De Schutter
Stochastic, spatial reaction-diffusion simulations have been widely used in systems biology and computational neuroscience. However, the increasing scale and complexity of models and morphologies have exceeded the capacity of any serial implementation. This led to the development of parallel solutions that benefit from the boost in performance of modern supercomputers. In this paper, we describe an MPI-based, parallel operator-splitting implementation for stochastic spatial reaction-diffusion simulations with irregular tetrahedral meshes...
2017: Frontiers in Neuroinformatics
https://www.readbyqxmd.com/read/28237685/evaluation-of-a-new-handheld-point-of-care-blood-gas-analyser-using-100-equine-blood-samples
#10
David Bardell, Eleanor West, J Mark Senior
OBJECTIVES: To determine whether the Enterprise point-of-care blood analysis system (EPOC) produces results in agreement with two other blood gas analysers in regular clinical use (i-STAT and Radiometer ABL77) and to investigate the precision of the new machine when used with equine whole blood. STUDY DESIGN: Prospective, randomized, non-blinded, comparative laboratory analyser study. ANIMALS: Horses admitted to a university teaching hospital requiring arterial or venous blood gas analysis as part of their routine clinical management...
February 22, 2017: Veterinary Anaesthesia and Analgesia
https://www.readbyqxmd.com/read/28237422/determination-of-pegfilgrastim-aggregates-by-size-exclusion-high-performance-liquid-chromatography-on-a-methacrylate-based-column
#11
Majid Shahbazi, Elnaz Tamaskany Zahedy, Shiva Kiumarsi, Mojtaba Hadi Soltanabad, Saleh Shahbazi Azar, Hossein Amini
A size-exclusion high-performance liquid chromatographic method using a methacrylate-based column was developed, validated and implemented for the determination of pegfilgrastim aggregates. The samples were directly injected into a TSKgel G4000PWXL column (7.5 mm × 300 mm, 10 μm, <500 A°) with a mobile phase of 100 mM phosphate, pH 2.5. Detection was made at 215 nm and analyses were run at a flow-rate of 0.6 ml/min at 10 °C. Vortex-mixing of samples produced oligomers, however, very high molecular weight aggregates were formed at high temperatures...
February 22, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28237269/catalytic-ozonation-of-paracetamol-using-commercial-and-pt-supported-nanocomposites-of-al2o3-the-impact-of-ultrasound
#12
Asu Ziylan-Yavaş, Nilsun H Ince
The study is the assessment of commercial γ-Al2O3 and its sonolytically modified nanocomposite in catalytic ozonation of paracetamol (PCT), which is an emerging water contaminant and a highly reactive compound with ozone. The results showed that commercial alumina was ineffective regardless of the solution pH, due to the low affinity of the catalyst surface for PCT and the high reactivity of the solute with molecular ozone. The modified catalyst, which was synthesized by decoration of the original surface with nanoparticles of platinum provided considerable improvement in the performance of the catalyst, particularly in mineralization of the target compound...
February 16, 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28237187/comparative-assessment-of-knee-joint-models-used-in-multi-body-kinematics-optimisation-for-soft-tissue-artefact-compensation
#13
Vincent Richard, Aurelio Cappozzo, Raphaël Dumas
Estimating joint kinematics from skin-marker trajectories recorded using stereophotogrammetry is complicated by soft tissue artefact (STA), an inexorable source of error. One solution is to use a bone pose estimator based on multi-body kinematics optimisation (MKO) embedding joint constraints to compensate for STA. However, there is some debate over the effectiveness of this method. The present study aimed to quantitatively assess the degree of agreement between reference (i.e., artefact-free) knee joint kinematics and the same kinematics estimated using MKO embedding six different knee joint models...
January 31, 2017: Journal of Biomechanics
https://www.readbyqxmd.com/read/28236626/selecting-multi-rule-quality-control-procedures-based-on-patient-risk
#14
Hassan Bayat
BACKGROUND: Traditionally, statistical quality control (SQC) planning is aimed at preventing the error rate from exceeding a pre-defined acceptable rate (Westgard JO. Basic QC Practices, 4th ed. Westgard QC, 2016). A pivotal characteristic for planning a QC procedure with the traditional approach is the probability of rejecting an analytical run that contains critical size errors (Pedc). Multi-rule QC procedures, with fully documented power curves, are important tools for SQC. In addition, it has been recommended (Parvin CA, Gronowski AM...
February 25, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28236460/phenolic-profiling-of-rooibos-using-off-line-comprehensive-normal-phase-countercurrent-chromatography%C3%A3-reversed-phase-liquid-chromatography
#15
Nico A Walters, André de Villiers, Elizabeth Joubert, Dalene de Beer
Limited performance of one dimensional chromatographic methods provides the incentive for the development of multidimensional chromatographic techniques for the analysis of complex mixtures. Rooibos (Aspalathus linearis) represents such a complex sample, containing a range of phenolic compounds that cannot be separated and identified using a single chromatographic technique within a reasonable run time. By implementing NP high performance countercurrent chromatography (NP-HPCCC) in the first dimension ((1)D) and RP ultra-high pressure LC (RP-UHPLC) in the second dimension ((2)D), a highly orthogonal (∼80%) off-line comprehensive two-dimensional separation of rooibos phenolic compounds was achieved in a total analysis time of 17h...
February 14, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28236327/analytical-evaluation-of-the-adams-%C3%A2-a1c-ha8180t-analyzer-for-the-measurement-of-hba1c
#16
Eloísa Urrechaga
BACKGROUND: The ADAMS(™) A1c HA-8180T (ARKRAY, Inc., Kyoto, Japan) is a high pressure liquid chromatography system designed for separation and quantitation of HbA1c, while detecting hemoglobin (Hb) variants and HbA2 , with an analysis time of just 3.5 min. The aim was to evaluate the analytical performance of this analyzer for routine HbA1c quantitation. METHODS: Trueness, imprecision, carry over, linearity, the effect of anemia and correlation with ADAMS(™) A1c HA-8180V (in Variant mode) were assessed according to guidelines of the Clinical and Laboratory Standards Institute (CLSI) and manufacturer...
February 25, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28236240/binding-specificity-profiles-from-computational-peptide-screening
#17
Stefan Wallin
The computational peptide screening method is a Monte Carlo-based procedure to systematically characterize the specificity of a peptide-binding site. The method is based on a generalized-ensemble algorithm in which the peptide sequence has become a dynamic variable, i.e., molecular simulations with ordinary conformational moves are enhanced with a type of "mutational" move such that proper statistics are achieved for multiple sequences in a single run. The peptide screening method has two main steps. In the first, reference simulations of the unbound state are performed and used to parametrize a linear model of the unbound state free energy, determined by requiring that the marginal distribution of peptide sequences is approximately flat...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28236233/application-of-the-attract-coarse-grained-docking-and-atomistic-refinement-for-predicting-peptide-protein-interactions
#18
Christina Schindler, Martin Zacharias
Peptide-protein interactions are abundant in the cell and form an important part of the interactome. Large-scale modeling of peptide-protein complexes requires a fully blind approach; i.e., simultaneously predicting the peptide-binding site and the peptide conformation to high accuracy. Here, we present one of the first fully blind peptide-protein docking protocols, pepATTRACT. It combines a coarse-grained ensemble docking search of the entire protein surface with two stages of atomistic flexible refinement...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28236183/validation-of-a-google-street-view-based-neighborhood-disorder-observational-scale
#19
Miriam Marco, Enrique Gracia, Manuel Martín-Fernández, Antonio López-Quílez
Recently, there has been a growing interest in developing new tools to measure neighborhood features using the benefits of emerging technologies. This study aimed to assess the psychometric properties of a neighborhood disorder observational scale using Google Street View (GSV). Two groups of raters conducted virtual audits of neighborhood disorder on all census block groups (N = 92) in a district of the city of Valencia (Spain). Four different analyses were conducted to validate the instrument. First, inter-rater reliability was assessed through intraclass correlation coefficients, indicating moderated levels of agreement among raters...
February 24, 2017: Journal of Urban Health: Bulletin of the New York Academy of Medicine
https://www.readbyqxmd.com/read/28235857/combined-long-term-caffeine-intake-and-exercise-inhibits-the-development-of-diabetic-nephropathy-in-oletf-rats
#20
Masato Suzuki, Daisuke Shindo, Ryuichiro Suzuki, Yoshiaki Shirataki, Hidefumi Waki
This study was performed to examine the effects of long-term caffeine-intake, with and without exercise, on the progression of diabetic nephropathy (DN) in an obese diabetic rat model. Thirty-two male Otsuka Long Evans Tokushima Fatty (OLETF) rats were assigned to sedentary (OLETF-Sed), exercise (OLETF-Ex), caffeine-intake (OLETF-Caf), and combined (OLETF-Caf & Ex) groups. Caffeine-intake groups were fed rat chow containing caffeine (90.7 ± 4.7 mg/kg/day). The OLETF-Ex and OLETF-Caf & Ex groups were able to run voluntarily at any time using a rotatory wheel...
February 23, 2017: Journal of Applied Physiology
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