keyword
MENU ▼
Read by QxMD icon Read
search

Tat

keyword
https://www.readbyqxmd.com/read/28550120/hiv-1-decreases-nrf2-are-activity-and-phagocytic-function-in-alveolar-macrophages
#1
Bashar S Staitieh, Lingmei Ding, Wendy A Neveu, Paul Spearman, David M Guidot, Xian Fan
Respiratory complications occur frequently in individuals living with human immunodeficiency-1 virus (HIV) infection, and there is evidence that HIV-related oxidative stress impairs alveolar macrophage immune function. We hypothesized that nuclear factor (erythroid-derived 2)-like 2 (Nrf2), a master transcription factor that activates the antioxidant response element (ARE) and regulates antioxidant defenses, has an important role in alveolar macrophage (AMs) immune dysfunction in individuals with HIV infections...
May 26, 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/28550068/age-specific-trends-of-atrial-fibrillation-related-ischaemic-stroke-and-transient-ischaemic-attack-anticoagulant-use-and-risk-factor-profile-in-chinese-population-a-15-year-study
#2
Yannie Soo, Nathan Chan, Kam Tat Leung, Xiang-Yan Chen, Vincent Mok, Lawrence Wong, Thomas Leung
BACKGROUND: Prevalence of atrial fibrillation (AF) is increasing globally, and the fivefold increase in stroke risk constitutes significant healthcare burden. AIMS: We aim to evaluate the trends of AF-related stroke and transient ischaemic attack (AF-stroke/TIA), prior anticoagulant use and their risk factors in different age groups in Chinese population. METHODS: Data were retrieved from the stroke registry at Prince of Wales Hospital. We compared the data at 5-year intervals over a 15-year period (years 1999, 2004, 2009 and 2014)...
May 26, 2017: Journal of Neurology, Neurosurgery, and Psychiatry
https://www.readbyqxmd.com/read/28549840/phosphatidylinositol-4-5-bisphosphate-mediated-pathophysiological-effect-of-hiv-1-tat-protein
#3
REVIEW
Bruno Beaumelle, Petra Tóth, Olfat A Malak, Christophe Chopard, Gildas Loussouarn, Nicolas Vitale
Human immunodeficiency virus (HIV)-infected cells actively release the transcriptional activator (Tat) viral protein that is required for efficient HIV gene transcription. Extracellular Tat is able to enter uninfected cells. We recently reported that internalized Tat escapes endosomes to reach the cytosol and is then recruited to the plasma membrane by phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). As a consequence, Tat strongly impairs different critical cellular functions in several cell types. Here we will review recent evidences showing that Tat, by affecting the interaction of key cellular effectors with PtdIns(4,5)P2, blocks exocytosis from neuroendocrine cells, perturbs the synaptic vesicle exo-endocytosis cycle, prevents efficient phagocytosis by macrophages, and alters potassium channel activity in cardiac cells...
May 23, 2017: Biochimie
https://www.readbyqxmd.com/read/28545384/synthesis-and-analgesic-activity-of-amines-combining-diazaadamantane-and-monoterpene-fragments
#4
Konstantin Ponomarev, Ekaterina Morozova, Alla Pavlova, Evgeniy Suslov, Dina Korchagina, Andrej Nefedov, Tat Yana Tolstikova, Konstantin Volcho, Nariman Salakhutdinov
BACKGROUND: It was found earlier that compounds combining diazaadamantane and monoterpene moieties possessed promising analgesic activity along with low acute toxicity and the lack of ulcerogenic activity. OBJECTIVE: In this paper, new structural analogues of the most active compounds were synthesized and evaluated for their analgesic activity. METHODS: Their structures were confirmed by various analytical methods, such as 1H and 13C NMR, HRMS...
May 25, 2017: Medicinal Chemistry
https://www.readbyqxmd.com/read/28544922/efficacy-of-benzalkonium-chloride-against-bioluminescent-p-aeruginosa-atcc9027-constructs
#5
N Shah, D C Naseby
Whole cell biosensors have been seldom used in the pharmaceutical and cosmetics industries for preservative efficacy testing (PET). According to several pharmacopoeias, preservatives should be tested for microbial activity using traditional viable count techniques; the use of whole cell microbial biosensors potentially provides an alternative, fast, and efficient method. The aim of the study was to assess the applicability of Pseudomonas aeruginosa ATCC9027 and its validated bioluminescent strains for preservative efficacy tests using benzalkonium chloride (BKC)...
April 23, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28542502/in-vivo-transduction-of-neurons-with-tat-uch-l1-protects-brain-against-controlled-cortical-impact-injury
#6
Hao Liu, Marie E Rose, Xiecheng Ma, Sherman Culver, C Edward Dixon, Steven H Graham
Many mechanisms or pathways are involved in secondary post-traumatic brain injury, such as the ubiquitin-proteasome pathway (UPP), axonal degeneration and neuronal cell apoptosis. UCH-L1 is a protein that is expressed in high levels in neurons and may have important roles in the UPP, autophagy and axonal integrity. The current study aims to evaluate the role of UCH-L1 in post-traumatic brain injury (TBI) and its potential therapeutic effects. A novel protein was constructed that fused the protein transduction domain (PTD) of trans-activating transduction (TAT) protein with UCH-L1 (TAT-UCH-L1) in order to promote neuronal transduction...
2017: PloS One
https://www.readbyqxmd.com/read/28540851/from-xe-2100-to-xn-9000-from-sis-standard-to-gfhc-recommendations-for-slide-review-potential-impact-on-review-rate-and-turnaround-time
#7
Anne Tamigniau, Nicolas Bailly, Bernard Chatelain, François Mullier
In 2014, we moved from XE-2100 with Standard SIS rules to XN-9000 with GFHC recommendations for slide review. The aim of our study was to evaluate the potential impact of the new Sysmex(®) automation with implementation of GFHC rules on our review rate, turnaround time (TAT), workload and on CD34+ stem cells enumeration. We conducted a retrospective observational study from September 2013 through August 2014, in CHU Dinant Godinne UCL Namur. With the GFHC recommendations and the new Sysmex(®) automation, we significantly reduced our review rate by nearly 30% considering all units (35...
June 1, 2017: Annales de Biologie Clinique
https://www.readbyqxmd.com/read/28540537/rapid-diagnosis-of-acute-norovirus-associated-gastroenteritis-evaluation-of-the-xpert-norovirus-assay-and-its-implementation-as-a-24-7-service-in-three-hospitals-in-j%C3%A3-nk%C3%A3-ping-county-sweden
#8
A J Henningsson, A Nilsson Bowers, J Nordgren, M Quttineh, A Matussek, S Haglund
Noroviruses are a leading cause of epidemic and sporadic cases of acute gastroenteritis worldwide. The rapid diagnosis of norovirus infection is important for prompt infection control measures and may reduce the need for additional diagnostic testing. Here we evaluated the performance of the rapid Xpert Norovirus assay, and assessed the turn-around time (TAT) before and after the implementation of the analysis as a 24/7 service at all the three hospitals in Jönköping County, Sweden. We describe the implementation process which was performed in two steps during 2014...
May 24, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28539656/pkc-%C3%AE-isoform-plays-a-crucial-role-in-tat-tlr4-signalling-pathway-to-activate-nf-%C3%AE%C2%BAb-and-cxcl8-production
#9
Manutea Serrero, Rémi Planès, Elmostafa Bahraoui
HIV-1 Tat protein induces the production of CXCL8 chemokine in a TLR4/MD2 and PKC dependent manner. The objective of this study was to understand whether these two pathways were distinct or constituted a single common pathway, and to determine the nature of the PKC isoforms involved and their interrelation with the activation of NF-κB and CXCL8 gene product expression. Here, we show that Tat-induced CXCL8 production is essentially dependent on the activation of PKC delta isoform, as shown a) by the capacity of PKC delta dominant negative (DN), and Rottlerin, a selective PKC delta pharmacological inhibitor, to inhibit Tat-induced CXCL8 production and b) by the ability of the constitutively active (CAT) isoform of PKC delta to induce CXCL8 production in a HEK cell line in the absence of Tat stimulation...
May 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28537425/differential-codon-usage-pattern-of-hiv-1-tat-gene-in-those-with-slower-and-faster-rates-of-disease-progression
#10
K K Vidyavijayan, Brahmaiah Anangi, Brindha B, Kannan T, Hassan S, Luke Elizabeth Hanna
Codon usage has been identified as one of the most important factors that influence gene expression. The frequencies with which the different codons are used vary significantly between different organisms and also between the genes within the same organism. Human immunodeficiency virus (HIV) has a remarkable nucleotide composition with an above average percentage of 'A' nucleotides resulting in a codon usage pattern different from that of the human host. Here we compared the codon usage pattern of HIV-1 genes among different groups of HIV disease progressors to understand the influence of differential codon usage pattern on the pathogenic manifestation in the host...
May 24, 2017: AIDS Research and Human Retroviruses
https://www.readbyqxmd.com/read/28530637/cell-penetrating-peptides-selectively-targeting-smad3-inhibit-profibrotic-tgf-%C3%AE-signaling
#11
Jeong-Han Kang, Mi-Yeon Jung, Xueqian Yin, Mahefatiana Andrianifahanana, Danielle M Hernandez, Edward B Leof
TGF-β is considered a master switch in the pathogenesis of organ fibrosis. The primary mediators of this activity are the SMAD proteins, particularly SMAD3. In the current study, we have developed a cell-penetrating peptide (CPP) conjugate of the HIV TAT protein that is fused to an aminoterminal sequence of sorting nexin 9 (SNX9), which was previously shown to bind phosphorylated SMAD3 (pSMAD3). We determined that specifically preventing the nuclear import of pSMAD3 using the TAT-SNX9 peptide inhibited profibrotic TGF-β activity in murine cells and human lung fibroblasts as well as in vivo with no demonstrable toxicity...
May 22, 2017: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/28529049/heterogeneity-of-transverse-axial-tubule-system-in-mouse-atria-remodeling-in-atrial-specific-na-ca-2-exchanger-knockout-mice
#12
Xin Yue, Rui Zhang, Brian Kim, Aiqun Ma, Kenneth D Philipson, Joshua I Goldhaber
Transverse-axial tubules (TATs) are commonly assumed to be sparse or absent in atrial myocytes from small animals. Atrial myocytes from rats, cats and rabbits lack TATs, which results in a characteristic "V"-shaped Ca release pattern in confocal line-scan recordings due to the delayed rise of Ca in the center of the cell. To examine TAT expression in isolated mouse atrial myocytes, we loaded them with the membrane dye Di-4-ANEPPS to label TATs. We found that >80% of atrial myocytes had identifiable TATs...
May 18, 2017: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/28525975/venous-thromboembolism-in-hospitalized-patients-receiving-chemotherapy-for-malignancies-at-japanese-community-hospital-prospective-observational-study
#13
Hiromitsu Kitayama, Tomohiro Kondo, Junko Sugiyama, Kazutomo Kurimoto, Yasuhiro Nishino, Michiaki Hirayama, Yasushi Tsuji
BACKGROUND: Although Asian population was recognized to have a lower risk of venous thromboembolism (VTE), its increasing prevalence and incidence remain unclear in patients with malignancies. We attempted to predict VTE development using activation markers of coagulation and fibrinolysis. METHODS: We enrolled patients with malignancy admitted to Tonan Hospital between April and December 2014 to receive a new-for-them chemotherapy regimen. All patients were examined for VTE by computed tomography and whole-leg compression ultrasonography before chemotherapy and three months later...
May 19, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28515319/the-h-region-of-twin-arginine-signal-peptides-supports-productive-binding-of-bacterial-tat-precursor-proteins-to-the-tatbc-receptor-complex
#14
Agnes Ulfig, Julia Fröbel, Frank Lausberg, Anne-Sophie Blümmel, Anna Katharina Heide, Matthias Müller, Roland Freudl
The twin arginine translocation (Tat) pathway transports folded proteins across bacterial membranes. Tat precursor proteins possess a conserved twin-arginine (RR) motif in their signal peptides that is involved in their binding to the Tat translocase, but some facets of this interaction remain unclear. Here, we investigated the role of the hydrophobic (h-) region of the Escherichia coli TMAO reductase (TorA) signal peptide in TatBC receptor binding in vivo and in vitro. We show that besides the RR motif, a minimal functional h-region in the signal peptide is required for Tat-dependent export in E...
May 17, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28513434/a-unifying-mechanism-for-the-biogenesis-of-membrane-proteins-co-operatively-integrated-by-the-sec-and-tat-pathways
#15
Fiona J Tooke, Marion Babot, Govind Chandra, Grant Buchanan, Tracy Palmer
The majority of multi-spanning membrane proteins are co-translationally inserted into the bilayer by the Sec pathway. An important subset of membrane proteins have globular, cofactor-containing extracytoplasmic domains requiring the dual action of the co-translational Sec and post-translational Tat pathways for integration. Here, we identify further unexplored families of membrane proteins that are dual Sec-Tat-targeted. We establish that a predicted heme-molybdenum cofactor-containing protein, and a complex polyferredoxin, each require the concerted action of two translocases for their assembly...
May 17, 2017: ELife
https://www.readbyqxmd.com/read/28509332/myeloid-but-not-epithelial-tissue-factor-exerts-protective-anti-inflammatory-properties-in-acid-aspiration-induced-acute-lung-injury
#16
J B Kral-Pointner, W C Schrottmaier, V Horvath, H Datler, L Hell, C Ay, B Niederreiter, B Jilma, J A Schmid, A Assinger, N Mackman, S Knapp, G Schabbauer
INTRODUCTION: Acute lung injury (ALI) is a life-threatening condition characterized by damaged alveolar-capillary structures and activation of inflammatory and hemostatic processes. Tissue factor (TF) represents a crucial link between inflammation and coagulation, as inflammatory mediators induce myeloid TF expression and TF initiates the extrinsic coagulation. OBJECTIVE: Since pulmonary inflammation stimulates TF expression and TF modulates immune responses, we aimed to elucidate its impact on ALI...
May 16, 2017: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/28507142/quantitative-assessment-of-passive-electrical-properties-of-the-cardiac-t-tubular-system-by-frap-microscopy
#17
M Scardigli, C Crocini, C Ferrantini, T Gabbrielli, L Silvestri, R Coppini, C Tesi, E A Rog-Zielinska, P Kohl, E Cerbai, C Poggesi, F S Pavone, L Sacconi
Well-coordinated activation of all cardiomyocytes must occur on every heartbeat. At the cell level, a complex network of sarcolemmal invaginations, called the transverse-axial tubular system (TATS), propagates membrane potential changes to the cell core, ensuring synchronous and uniform excitation-contraction coupling. Although myocardial conduction of excitation has been widely described, the electrical properties of the TATS remain mostly unknown. Here, we exploit the formal analogy between diffusion and electrical conductivity to link the latter with the diffusional properties of TATS...
May 15, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28506965/explaining-the-obesity-paradox-the-association-between-body-composition-and-colorectal-cancer-survival-c-scans-study
#18
Bette J Caan, Jeffrey A Meyerhardt, Candyce H Kroenke, Stacey Alexeeff, Jingjie Xiao, Erin Weltzien, Elizabeth Cespedes Feliciano, Adrienne L Castillo, Charles P Quesenberry, Marilyn L Kwan, Carla M Prado
Background: Body composition may partially explain the U-shaped association between body mass index (BMI) and colorectal cancer survival.Methods: Muscle and adiposity at colorectal cancer diagnosis and survival were examined in a retrospective cohort using Kaplan-Meier curves, multivariable Cox regression, and restricted cubic splines in 3,262 early-stage (I-III) male (50%) and female (50%) patients. Sarcopenia was defined using optimal stratification and sex- and BMI-specific cut points. High adiposity was defined as the highest tertile of sex-specific total adipose tissue (TAT)...
May 15, 2017: Cancer Epidemiology, Biomarkers & Prevention
https://www.readbyqxmd.com/read/28504936/wave-mode-discrimination-of-coded-ultrasonic-guided-waves-using-two-dimensional-compressed-pulse-analysis
#19
Sergio Malo, Sina Fateri, Makis Livadas, Cristinel Mares, Tat-Hean Gan
Ultrasonic guided waves testing is a technique successfully used in many industrial scenarios worldwide. For many complex applications, the dispersive nature and multimode behavior of the technique still poses a challenge for correct defect detection capabilities. In order to improve the performance of the guided waves a 2-D compressed pulse analysis is presented in this paper. This novel technique combines the use of pulse compression and dispersion compensation in order to improve the signal-tonoise ratio and temporal-spatial resolution of the signals...
May 11, 2017: IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
https://www.readbyqxmd.com/read/28503428/soluble-expression-of-recomb-inant-cmyc-klf4-oct4-and-sox2-proteins-in-bacteria-and-transduction-into-living-cells
#20
Guo-Dan Liu, Shi-Feng Zhou, Xu-Chen Ding, Chun-Lai Fang, Shu-Yong Mi, Xiang-Chun Gao, Qing Han
AIM: To develop a new method to produce recombinant reprogramming proteins, cMyc, Klf4, Oct4, and Sox2, in soluble format with low cost for the generation of induced pluripotent stem cells (iPSCs). METHODS: A short polypeptide sequence derived from the HIV trans-activator of transcription protein (TAT) and the nucleus localization signal (NLS) polypeptide were fused to the N terminus of the reprogramming proteins and they were constructed into pCold-SUMO vector which can extremely improve the solubility of recombinant proteins...
2017: International Journal of Ophthalmology
keyword
keyword
20231
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"