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https://www.readbyqxmd.com/read/28230505/morphology-and-cell-death-of-human-leukaemia-t-lymphocytes-jurkat-cells-expressing-hiv-1-tat-72
#1
J Gilloteaux, J Koch, J M Jamison
No abstract text is available yet for this article.
January 2017: Ultrastructural Pathology
https://www.readbyqxmd.com/read/28230065/in-vivo-evaluation-of-a-novel-format-of-a-bivalent-her3-targeting-and-albumin-binding-therapeutic-affibody-construct
#2
Tarek Z Bass, Maria Rosestedt, Bogdan Mitran, Fredrik Y Frejd, John Löfblom, Vladimir Tolmachev, Stefan Ståhl, Anna Orlova
Overexpression of human epidermal growth factor receptor 3 (HER3) is involved in resistance to several therapies for malignant tumours. Currently, several anti-HER3 monoclonal antibodies are under clinical development. We introduce an alternative approach to HER3-targeted therapy based on engineered scaffold proteins, i.e. affibody molecules. We designed a small construct (22.5 kDa, denoted 3A3), consisting of two high-affinity anti-HER3 affibody molecules flanking an albumin-binding domain ABD, which was introduced for prolonged residence in circulation...
February 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28229590/impacts-of-crosslinkers-on-biological-effects-of-mesoporous-silica-nanoparticles
#3
Yi-Ping Chen, Si-Han Wu, I-Chih Chen, Chien-Tsu Chen
Chemically synthesized crosslinkers play decisive roles in variable cargos attached to nanoparticles (NPs). Previous studies reported that surface properties, such as the size, charge, and surface chemistry, are particularly important determinants influencing the biological fate and actions of NPs and cells. Recent studies also focused on the relationship of serum proteins with the surface properties of NPs (also called the protein corona), which is recognized as a key factor in determining the cytotoxicity and biodistribution...
February 23, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28223937/orthostatic-challenge-shifts-the-hemostatic-system-of-patients-recovered-from-stroke-toward-hypercoagulability
#4
Gerhard Cvirn, Markus Kneihsl, Christine Rossmann, Margret Paar, Thomas Gattringer, Axel Schlagenhauf, Bettina Leschnik, Martin Koestenberger, Erwin Tafeit, Gilbert Reibnegger, Irhad Trozic, Andreas Rössler, Franz Fazekas, Nandu Goswami
Aims: The objective of our study was to assess the effects of orthostatic challenge on the coagulation system in patients with a history of thromboembolic events and to assess how they compared with age-matched healthy controls. Methods: Twenty-two patients with histories of ischemic stroke and 22 healthy age-matched controls performed a sit-to-stand test. Blood was collected prior to- and at the end of- standing in the upright position for 6 min. Hemostatic profiling was performed by determining thrombelastometry and calibrated automated thrombogram values, indices of thrombin generation, standard coagulation times, markers of endothelial activation, plasma levels of coagulation factors and copeptin, and hematocrit...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28223511/a-signal-sequence-suppressor-mutant-that-stabilizes-an-assembled-state-of-the-twin-arginine-translocase
#5
Qi Huang, Felicity Alcock, Holger Kneuper, Justin C Deme, Sarah E Rollauer, Susan M Lea, Ben C Berks, Tracy Palmer
The twin-arginine protein translocation (Tat) system mediates transport of folded proteins across the cytoplasmic membrane of bacteria and the thylakoid membrane of chloroplasts. The Tat system of Escherichia coli is made up of TatA, TatB, and TatC components. TatBC comprise the substrate receptor complex, and active Tat translocases are formed by the substrate-induced association of TatA oligomers with this receptor. Proteins are targeted to TatBC by signal peptides containing an essential pair of arginine residues...
February 21, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28223147/comparative-performance-study-of-six-commercial-molecular-assays-for-rapid-detection-of-toxigenic-clostridium-difficile
#6
Yossi Paitan, Tamar Miller Roll, Amos Adler
OBJECTIVES: Rapid and accurate detection of C. difficile in stool impacts patient treatment and containment efforts. Detection of C. difficile toxin genes using nucleic acid amplification techniques (NAAT) is part of a multistep algorithm. Our objective was to directly compare the diagnostic accuracy and applicability of 6 commercial C. difficile NAAT assays. METHODS: In this study, 210 specimens were analysed in parallel by 6 commercial NAAT. Toxigenic culture was used as a reference method...
February 18, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28222947/comparison-of-the-effectiveness-of-three-different-treatment-methods-for-temporomandibular-joint-disc-displacement-without-reduction
#7
U Tatli, M E Benlidayi, O Ekren, F Salimov
The aim of this study was to compare the effectiveness of three treatment methods for unilateral temporomandibular joint (TMJ) disc displacement without reduction (DDwoR). One hundred and twenty patients with unilateral TMJ DDwoR were assigned randomly to one of three treatment groups (40 patients in each): group 1 received arthrocentesis, group 2 received stabilization splint therapy following arthrocentesis, and group 3 received splint therapy only. The groups were compared in terms of pain (visual analogue scale), joint function (maximum mouth opening and laterotrusive movements), disability and psychological status (validated questionnaire), and success rates...
February 17, 2017: International Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/28222086/equivalence-study-of-two-temperature-measurement-methods-in-febrile-adult-patients-with-cancer
#8
Tina M Mason, Abdelbaki Boubekri, Jennifer Lalau, Amy Patterson, Susan R Hartranft, Steven K Sutton
PURPOSE/OBJECTIVES: To assess equivalence of temperatures taken via temporal artery and oral methods in febrile inpatients with cancer.
. DESIGN: Repeated measures equivalence design.
. SETTING: 24-bed hematology-oncology unit and 36-bed blood and marrow transplantation unit at H. Lee Moffitt Cancer Center and Research Institute, a National Cancer Institute-designated comprehensive cancer center in Tampa, Florida.
. SAMPLE: Convenience sample of 58 febrile inpatients using 60 temperature measurements...
March 1, 2017: Oncology Nursing Forum
https://www.readbyqxmd.com/read/28221993/shading-based-surface-detail-recovery-under-general-unknown-illumination
#9
Di Xu, Qi Duan, Jianmin Zheng, Juyong Zhang, Jianfei Cai, Tat-Jen Cham
Reconstructing the shape of a 3D object from multi-view images under unknown, general illumination is a fundamental problem in computer vision and high quality reconstruction is usually challenging especially when fine detail is needed and the albedo of the object is non-uniform. This paper introduces vertex overall illumination vectors to model the illumination effect and presents a total variation (TV) based approach for recovering surface details using shading and multi-view stereo (MVS). Behind the approach are the two important observations: (1) the illumination over the surface of an object often appears to be piece wise smooth and (2) the recovery of surface orientation is not sufficient for reconstructing the surface, which was often overlooked previously...
February 17, 2017: IEEE Transactions on Pattern Analysis and Machine Intelligence
https://www.readbyqxmd.com/read/28218748/hiv-tat-protein-and-amyloid-%C3%AE-peptide-form-multifibrillar-structures-that-cause-neurotoxicity
#10
Alina Hategan, Mario A Bianchet, Joseph Steiner, Elena Karnaukhova, Eliezer Masliah, Adam Fields, Myoung-Hwa Lee, Alex M Dickens, Norman Haughey, Emilios K Dimitriadis, Avindra Nath
Deposition of amyloid-β plaques is increased in the brains of HIV-infected individuals, and the HIV transactivator of transcription (Tat) protein affects amyloidogenesis through several indirect mechanisms. Here, we investigated direct interactions between Tat and amyloid-β peptide. Our in vitro studies showed that in the presence of Tat, uniform amyloid fibrils become double twisted fibrils and further form populations of thick unstructured filaments and aggregates. Specifically, Tat binding to the exterior surfaces of the Aβ fibrils increases β-sheet formation and lateral aggregation into thick multifibrillar structures, thus producing fibers with increased rigidity and mechanical resistance...
February 20, 2017: Nature Structural & Molecular Biology
https://www.readbyqxmd.com/read/28218730/neoflavonoids-as-inhibitors-of-hiv-1-replication-by-targeting-the-tat-and-nf-%C3%AE%C2%BAb-pathways
#11
Dionisio A Olmedo, José Luis López-Pérez, Esther Del Olmo, Luis M Bedoya, Rocío Sancho, José Alcamí, Eduardo Muñoz, Arturo San Feliciano, Mahabir P Gupta
Twenty-eight neoflavonoids have been prepared and evaluated in vitro against HIV-1. Antiviral activity was assessed on MT-2 cells infected with viral clones carrying the luciferase reporter gene. Inhibition of HIV transcription and Tat function were tested on cells stably transfected with the HIV-LTR and Tat protein. Seven 4-phenylchromen-2-one derivatives showed HIV transcriptional inhibitory activity but only the phenylchrome-2-one 10 inhibited NF-κB and displayed anti-Tat activity simultaneously. Compounds 10, 14, and 25, inhibited HIV replication in both targets at concentrations <25 μM...
February 19, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28214048/psychosocial-and-behavioral-functioning-and-their-relationship-to-seizure-timing-in-children-with-benign-epilepsy-with-centrotemporal-spikes
#12
Gonca Bektaş, Uğur Tekin, Edibe Pembegül Yıldız, Tuğçe Aksu Uzunhan, Burak Tatlı, Nur Aydınlı, Mine Çalışkan, Meral Özmen
BACKGROUND: Psychosocial and behavioral problems have been reported in children with benign epilepsy with centrotemporal spikes (BECTS). Distinctive features of typical BECTS associated with cognitive and behavioral problems have not clearly been defined. PURPOSE: We aimed to identify psychosocial and behavioral functioning and their relationship to seizure timing in BECTS. METHODS: Consecutive patients with BECTS were recruited from the pediatric neurology outpatient clinic between May 2015 and May 2016...
February 14, 2017: Brain & Development
https://www.readbyqxmd.com/read/28213560/identification-of-putative-substrates-of-sec2-a-chloroplast-inner-envelope-translocase
#13
Yubing Li, Jonathan R Martin, Giovanni A Aldama, Donna E Fernandez, Kenneth Cline
Most chloroplast proteins are synthesized in the cytosol and imported into chloroplasts. Many imported proteins are further targeted to the thylakoid membrane and lumen by the SEC1, TAT or SRP/ALB3 translocases. Others are targeted to the inner chloroplast envelope membrane by un-described translocase(s). Recently, a second SEC system (SEC2) consisting of SCY2, SECE2, and SECA2 was found in the chloroplast envelope. Null mutants of SCY2 in Arabidopsis exhibit a severe embryo lethal phenotype. To investigate the function of SEC2 system in plants, we used inducible RNAi to knockdown SCY2 in Arabidopsis...
February 17, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28208588/depicting-binding-mediated-translocation-of-hiv-1-tat-peptides-in-living-cells-with-nanoscale-pens-of-tat-conjugated-quantum-dots
#14
Chien Y Lin, Jung Y Huang, Leu-Wei Lo
Cell-penetrating peptides (CPPs) can translocate across cell membranes, and thus have great potential for the cellular delivery of macromolecular cargoes. However, the mechanism of this cellular uptake process is not yet fully understood. In this study, a time-lapse single-particle light-sheet microscopy technique was implemented to obtain a parallel visualization of the translocating process of individual human immunodeficiency virus 1 (HIV-1) transactivator of transcription (Tat) peptide conjugated quantum dots (TatP-QDs) in complex cellular terrains...
February 10, 2017: Sensors
https://www.readbyqxmd.com/read/28203075/co-delivery-of-paclitaxel-and-tos-cisplatin-via-tat-targeted-solid-lipid-nanoparticles-with-synergistic-antitumor-activity-against-cervical-cancer
#15
Bo Liu, Li Han, Junyan Liu, Shumei Han, Zhen Chen, Lixi Jiang
BACKGROUND: Cervical cancer is a major world health problem for women. Currently, cancer research focuses on improving therapy for cervical cancer using various treatment options such as co-delivery of chemotherapeutic agents by nanocarriers. PURPOSE: The aim of this study was to develop trans-activating transcriptional activator (TAT)-modified solid lipid nanoparticles (SLNs) for co-delivery of paclitaxel (PTX) and α-tocopherol succinate-cisplatin prodrug (TOS-CDDP) (TAT PTX/TOS-CDDP SLNs) in order to achieve synergistic antitumor activity against cervical cancer...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28202283/from-typical-sequences-to-typical-genotypes
#16
Omri Tal, Tat Dat Tran, Jacobus Portegies
We demonstrate an application of a core notion of information theory, typical sequences and their related properties, to analysis of population genetic data. Based on the asymptotic equipartition property (AEP) for nonstationary discrete-time sources producing independent symbols, we introduce the concepts of typical genotypes and population entropy and cross entropy rate. We analyze three perspectives on typical genotypes: a set perspective on the interplay of typical sets of genotypes from two populations, a geometric perspective on their structure in high dimensional space, and a statistical learning perspective on the prospects of constructing typical-set based classifiers...
February 12, 2017: Journal of Theoretical Biology
https://www.readbyqxmd.com/read/28202126/-effect-of-ulinastatin-on-serum-levels-of-tumor-necrosis-factor-%C3%AE-p-selectin-and-thrombin-antithrombin-complex-in-young-rats-with-sepsis
#17
Yun Liu, Xing-Heng Wu
OBJECTIVE: To investigate the effect of ulinastatin (UTI) for early drug intervention on the serum levels of tumor necrosis factor-α (TNF-α), P-selectin, and thrombin-antithrombin complex (TAT) in young rats with sepsis. METHODS: A total of 120 male rats aged 4 weeks were randomly divided into normal control group, sham-operation group, sepsis group, low-dose UTI group (50 000 U/kg), and high-dose UTI group (200 000 U/kg), with 24 rats in each group. Modified cecal ligation and puncture was performed to establish a rat model of sepsis, and the rats in the low- and high-dose UTI groups were given caudal vein injection of UTI after model establishment...
February 2017: Zhongguo Dang Dai Er Ke za Zhi, Chinese Journal of Contemporary Pediatrics
https://www.readbyqxmd.com/read/28198575/tat-biliverdin-reductase-a-protects-ins-1-cells-from-human-islet-amyloid-polypeptide-induced-cytotoxicity-by-alleviating-oxidative-stress-and-er-stress
#18
Su Jin Lee, Hyung Kyung Kang, Won Sik Eum, Jinseu Park, Soo Young Choi, Hyeok Yil Kwon
Human islet amyloid polypeptide (hIAPP), a major constituent of islet amyloid deposits, induces pancreatic β-cell apoptosis and eventually contributes to β-cell deficit in patients with type 2 diabetes mellitus (T2DM). In this study, Tat-mediated transduction of biliverdin reductase A (BLVRA) was investigated in INS-1 cells to examine whether exogenous supplementation of BLVRA prevented hIAPP-induced apoptosis and dysfunction in insulin secretion in β-cells. Tat-BLVRA fusion protein was efficiently delivered into INS-1 cells in a time- and dose-dependent manner...
February 15, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28197274/early-changes-in-the-antithrombin-and-thrombin-antithrombin-complex-in-patients-with-paroxysmal-atrial-fibrillation
#19
Mariya Negreva, Svetoslav Georgiev, Krasimira Prodanova, Julia Nikolova
BACKGROUND: Data on coagulation changes in paroxysmal atrial fibrillation (PAF) are scarce. The aim of this study was to examine plasma antithrombin (AT) levels and activity as well as thrombin-antithrombin (TAT) complex levels in the early hours of the clinical manifestation of PAF. METHODS: Fifty-one patients (26 men and 25 women; mean age 59.84 ± 1.60 years) were consecutively selected with PAF duration < 24 hours, and 52 controls (26 men and 26 women; mean age 59...
June 2016: Cardiology Research
https://www.readbyqxmd.com/read/28195891/simple-skin-stretching-device-in-assisted-tension-free-wound-closure
#20
Li-Fu Cheng, Jiunn-Tat Lee, Honda Hsu, Meng-Si Wu
BACKGROUND: Numerous conventional wound reconstruction methods, such as wound undermining with direct suture, skin graft, and flap surgery, can be used to treat large wounds. The adequate undermining of the skin flaps of a wound is a commonly used technique for achieving the closure of large tension wounds; however, the use of tension to approximate and suture the skin flaps can cause ischemic marginal necrosis. The purpose of this study is to use elastic rubber bands to relieve the tension of direct wound closure for simultaneously minimizing the risks of wound dehiscence and wound edge ischemia that lead to necrosis...
February 14, 2017: Annals of Plastic Surgery
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