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https://www.readbyqxmd.com/read/28921905/antibiotic-resistant-escherichia-coli-in-southeastern-australian-pig-herds-and-implications-for-surveillance
#1
L K van Breda, O P Dhungyel, M P Ward
To investigate public health implications of antibiotics to control post-weaning scours, we surveyed 22 commercial pig herds in southeastern Australia. Fifty faecal samples per herd were collected from pre- and post-weaned piglets. Presumptive Escherichia coli isolates were confirmed by MALDI-TOF MS. Isolates (n = 325) were screened for susceptibility to 19 veterinary antibiotics using MIC broth microdilution. All 325 E. coli isolates underwent further testing against 27 antibiotics used in human medicine and were screened for ETEC adhesin and enterotoxin genes (F4 (K88), F5 (K99), F6 (987P), F18, F41, STa, STb, Stx2e and LT) by multiplex PCR...
September 17, 2017: Zoonoses and Public Health
https://www.readbyqxmd.com/read/28920793/multi-drug-resistance-patterns-of-enteric-bacteria-in-two-populations-of-free-ranging-eastern-box-turtles-terrapene-carolina-carolina
#2
Cari Rasmussen, Matthew C Allender, Christopher A Phillips, John Byrd, Terrell Lloyd, Carol Maddox
Gram-negative isolates (n = 84) from 71% of free-ranging Eastern box turtles (Terrapene carolina carolina) in Illinois and Tennessee, United States, demonstrated resistance to at least one antibiotic while 30% of isolates demonstrated resistance to two or more antibiotics. Resistance was observed against cefoxitin, amoxicillin-clavulanic acid, cefazolin, ampicillin, ticarcillin, cefovecin, and ceftiofur. Gram-positive bacteria were isolated from 49 turtles, and all were observed to be resistant to two or more antibiotics...
September 2017: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
https://www.readbyqxmd.com/read/28919694/incidence-of-bovine-clinical-mastitis-in-jammu-region-and-antibiogram-of-isolated-pathogens
#3
Adil Majid Bhat, Jasvinder Singh Soodan, Rajiv Singh, Ishfaq Ahmad Dhobi, Tufail Hussain, Mohammad Yousuf Dar, Muheet Mir
AIM: This study was conducted to evaluate the incidence of clinical mastitis in bovines of Jammu region, to identify the infectious organisms responsible for it, and the antimicrobial sensitivity of isolated pathogens. MATERIALS AND METHODS: The study was conducted on cases that were presented to the Medicine Division of Teaching Veterinary Clinical Complex, Faculty of Veterinary Sciences and Animal Husbandry, R.S. Pura, Jammu, Jammu and Kashmir. A total of 260 cases of bovines were presented from June 30, 2012, to July 01, 2013, out of which 30 cases were of clinical mastitis...
August 2017: Veterinary World
https://www.readbyqxmd.com/read/28919687/presence-and-characterization-of-escherichia-coli-virulence-genes-isolated-from-diseased-pigs-in-the-central-region-of-argentina
#4
Fernando A Bessone, Gabriela Bessone, Sebastián Marini, María B Conde, Fabrisio E Alustiza, Gustavo Zielinski
BACKGROUND: The main pathogen of neonatal and post weaning diarrhea and edema disease (ED) is Escherichia coli and pathotypes involved are enterotoxigenic, enteropathogenic, and shiga toxigenic (ETEC, EPEC, and STEC, respectively). Those diseases cause economic loss in pig production. AIM: The aim of this work was to evaluate the presence of strains expressing virulence markers genes and the antibiotic susceptibility profiles of E. coli from clinical cases of post weaning diarrhea and ED in farms in the central area of Argentina...
August 2017: Veterinary World
https://www.readbyqxmd.com/read/28919674/prevalence-of-enteropathogens-and-their-antibiotic-sensitivity-pattern-in-puppies-with-hemorrhagic-gastroenteritis
#5
A Kokila Priya, M Balagangatharathilagar, D Chandrasekaran, M Parthiban, S Prathaban
AIM: Hemorrhagic gastroenteritis (HGE) ranging from mild to severe forms is commonly encountered in puppies. The aim of the study was to identify the prevalence of common enteropathogens and the antibiotic sensitivity pattern in puppies reported with HGE. MATERIALS AND METHODS: The canine HGE activity index, with little modification, was adopted to identify Grade III/severely affected puppies below 6 months of age. Fecal polymerase chain reaction (PCR) assay was employed to screen and compare the enteropathogens in puppies with hemorrhagic diarrhea and healthy control...
August 2017: Veterinary World
https://www.readbyqxmd.com/read/28919615/microbiology-of-chronic-suppurative-otitis-media-in-a-tertiary-care-hospital-in-bangladesh
#6
N Akhtar, P G Datta, M Yasmin, A Naha, A M Jewel, F Hossen, S Ahmed, U K Datta
This cross sectional prospective study was carried out in the Department of Otolaryngology-Head & Neck Surgery, Bangabandhu Sheikh Mujib Medical University (BSMMU) and Department of Microbiology, University of Dhaka from July 2012 to June 2013. The objectives of this study were to identify the common microorganisms involved and the antibiograms of chronic suppurative otitis media (CSOM) patients in this tertiary care hospital in Bangladesh. A total of 117 patients clinically diagnosed of CSOM were enrolled in the study...
July 2017: Mymensingh Medical Journal: MMJ
https://www.readbyqxmd.com/read/28919606/current-trends-of-using-antimicrobials-and-their-sensitivity-pattern-in-infectious-cases-at-department-of-orthopedics-in-a-tertiary-care-hospital
#7
M M Sarker, S K Saha, S Saha, A N Naushad, S Ajmery, P Roy, M S Chowdhury, L Mukta
This cross-sectional descriptive study was carried out to analyse the current trends of using antimicrobials at orthopaedics department in different surgical procedures and observe the antimicrobial sensitivity pattern of infective organisms in postoperative wound infection cases at the department of Orthopaedics, Mymensingh Medical College Hospital with collaboration of department of Pharmacology and Therapeutics in Mymensingh Medical College, Mymensingh from January 2016 to December 2016. Among 253 orthopaedics surgical cases Penicillins, Cephalosporins, and Aminoglycoside group were commonly prescribed antimicrobials that had been use postoperatively in most of the Orthopaedics cases...
July 2017: Mymensingh Medical Journal: MMJ
https://www.readbyqxmd.com/read/28919603/current-trends-of-using-antimicrobials-at-obstetrics-and-gynaecology-department-in-a-tertiary-care-hospital
#8
M M Islam, S K Saha, S Saha, U A Wahid, R R Gomes, K S Ahmed, A N Naushad, S E Suchi, C Saha, D Mondal
This cross-sectional descriptive study was done to analyse the current trends of using antimicrobials in various surgical procedures at Obstetrics and Gynaecology department in Mymensingh Medical College Hospital, Mymensingh from July 2014 to June 2015. Among 300 postoperative obstetrics and gynaecological surgery cases, samples were selected as non-random purposive selection method where pattern of using antimicrobials were analysed by SPSS method 20.1 versions. The incidence of antimicrobial resistance is on continued rise with a threat to return to the pre-antibiotic era...
July 2017: Mymensingh Medical Journal: MMJ
https://www.readbyqxmd.com/read/28916560/cmeabc-multidrug-efflux-pump-contributes-to-antibiotic-resistance-and-promotes-campylobacter-jejuni-survival-and-multiplication-in-acanthamoeba-polyphaga
#9
Ana Vieira, Amritha Ramesh, Alan M Seddon, Andrey V Karlyshev
Campylobacter jejuni is a foodborne pathogen recognized as the leading cause of human bacterial gastroenteritis. The wide use of antibiotics in medicine and in animal husbandry has led to an increased incidence of antibiotic resistance in Campylobacter In addition to a role in multidrug resistance, the Campylobacter CmeABC RND-type efflux pump, which is associated with multidrug resistance (MDR), may also be involved in virulence. As a vehicle of pathogenic microorganisms, the protozoan Acanthamoeba is a good model for the investigation of bacterial survival in the environment and molecular mechanisms of pathogenicity...
September 15, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28916193/ferulic-acid-promotes-survival-and-differentiation-of-neural-stem-cells-to-prevent-gentamicin-induced-neuronal-hearing-loss
#10
Lintao Gu, Xinhua Cui, Wei Wei, Jia Yang, Xuezhong Li
Neural stem cells (NSCs) have exhibited promising potential in therapies against neuronal hearing loss. Ferulic acid (FA) has been widely reported to enhance neurogenic differentiation of different stem cells. We investigated the role of FA in promoting NSC transplant therapy to prevent gentamicin-induced neuronal hearing loss. NSCs were isolated from mouse cochlear tissues to establish in vitro culture, which were then treated with FA. The survival and differentiation of NSCs were evaluated. Subsequently, neurite outgrowth and excitability of the in vitro neuronal network were assessed...
September 12, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28910943/-study-on-antibiotic-resistance-of-escherichia-coli-and-enterococcus-colonized-in-intestine-of-neonates-from-neonatal-intensive-care-unit
#11
X F Li, Z J Liu, X Chen, J Li, Z G Cui, B Kan, J R Ma, J H Cui
Objective: To understand the antibiotic resistance of bacteria colonized in intestine of the neonates from neonatal intensive care unit (NICU) and provide evidence to guide clinical antibiotic treatment. Methods: From May, 2014 to May, 2015, a total of 572 stool samples were collected from the neonates of NICU in our hospital. Escherichia coli and Enterococcus were detected with VITEK-2 system. Results: A total of 328 strains of E. coli and 243 strains of Enterococcus were isolated respectively in this study...
September 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/28906365/the-antibiotic-susceptibility-patterns-of-uropathogens-among-children-with-urinary-tract-infection-in-shiraz
#12
Gholamreza Pouladfar, Mitra Basiratnia, Mojtaba Anvarinejad, Pejman Abbasi, Fatemeh Amirmoezi, Samaneh Zare
Urinary tract infection (UTI) is one of the most common bacterial infections in pediatrics. Delay in diagnosis and treatment can cause significant morbidity. The physicians knowledge regarding the symptoms, microorganisms that caused UTI, and effective antibiotics in a geographical area can help them to select the appropriate antibiotics. This study was performed to determine the prevalence of bacteria that cause UTI and their susceptibility to common antibiotics as well as the common symptoms and associated factors in children of Shiraz, Southern Iran...
September 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28904298/usefulness-of-urinary-biomarkers-for-nephrotoxicity-in-cynomolgus-monkeys-treated-with-gentamicin-cisplatin-and-puromycin-aminonucleoside
#13
Hiroshi Uchino, Junko Fujishima, Kaori Fukuoka, Teppei Iwakiri, Akira Kamikuri, Hidenori Maeda, Kazuhiro Nakama
The objective of this study was to investigate the availability of novel urinary biomarkers (BMs) such as total protein, albumin, β2-microglobulin, clusterin, cystatin C, neutrophil gelatinase-associated lipocalin (NGAL) for the detection of acute nephrotoxicity in cynomolgus monkeys. Animals (total 9 males/3 groups) were administered gentamicin (GM) subcutaneously at 40 mg/kg for 7 days, cisplatin (CDDP) intravenously at 3 mg/kg once and puromycin aminonucleoside (PAN) intravenously at 20 mg/kg for 7 days...
2017: Journal of Toxicological Sciences
https://www.readbyqxmd.com/read/28903765/antimicrobial-resistance-among-pathogenic-bacteria-from-mink-neovison-vison-in-denmark
#14
Nanett Kvist Nikolaisen, Desireé Corvera Kløve Lassen, Mariann Chriél, Gitte Larsen, Vibeke Frøkjær Jensen, Karl Pedersen
BACKGROUND: For proper treatment of bacterial infections in mink, knowledge of the causative agents and their antimicrobial susceptibility patterns is crucial. The used antimicrobials are in general not registered for mink, i.e. most usage is "off-label". In this study, we report the patterns of antimicrobial resistance among pathogenic bacteria isolated from Danish mink during the period 2014-2016. The aim of this investigation was to provide data on antimicrobial resistance and consumption, to serve as background knowledge for new veterinary guidelines for prudent and optimal antimicrobial usage in mink...
September 13, 2017: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/28901726/antimicrobial-stewardship-in-paediatric-oncology-impact-on-optimising-gentamicin-use-in-febrile-neutropenia
#15
Stefanie Hennig, Christine E Staatz, Daniel Natanek, Sabina Bialkowski, Carolina Consuelo Llanos Paez, Rachael Lawson, Julia Clark
OBJECTIVES: To evaluate the impact of an antimicrobial stewardship (AMS) intervention, involving introduction of new guidelines on the treatment of febrile neutropenia (FN), on improving the use of gentamicin in paediatric oncology patients. DESIGN AND INTERVENTION: Updated guidelines for gentamicin usage in paediatrics with FN were implemented at a tertiary children's teaching hospital, in Brisbane, Australia. Data on gentamicin usage before and after the guideline change were collected retrospectively from children with cancer admitted to hospital with FN between January 2012 and December 2013...
September 13, 2017: Pediatric Blood & Cancer
https://www.readbyqxmd.com/read/28898907/effects-of-cobalamin-vitamin-b12-on-gentamicin-induced-nephrotoxicity-in-rat
#16
Saeed Hajihashemi, Zeinab Hamidizad, Ali Rahbari, Fatemeh Ghanbari, Zohre Aghaee Motealeghi
Background The main side effect of gentamicin is nephrotoxicity. The effect of cobalamin (Cob) was investigated on gentamicin nephrotoxicity in rats. Methods Renal injury induced by i.p. injection of gentamicin (100 mg/kg) for 8 consecutive days. Cobalamin (6 mg/kg/day, i.p) treatment was done for 8 consecutive days as co-treatment and post-treatment protocol. Results Cobalamin significantly increased creatinine clearance levels and renal blood flow which were reduced by gentamicin. Also, cobalamin significantly improved serum electrolytes (sodium and potassium) levels which were disturbed by gentamicin...
September 12, 2017: Drug Research
https://www.readbyqxmd.com/read/28898269/faecal-carriage-of-ctx-m-extended-spectrum-beta-lactamase-producing-enterobacteriaceae-among-street-children-dwelling-in-mwanza-city-tanzania
#17
Nyambura Moremi, Heike Claus, Ulrich Vogel, Stephen E Mshana
BACKGROUND: Data on ESBL carriage of healthy people including children are scarce especially in developing countries. We analyzed the prevalence and genotypes of ESBL-producing Enterobacteriaceae (EPE) in Tanzanian street children with rare contact to healthcare facilities but significant interactions with the environment, animals and other people. METHODOLOGY/ PRINCIPLE FINDINGS: Between April and July 2015, stool samples of 107 street children, who live in urban Mwanza were analyzed for EPE...
2017: PloS One
https://www.readbyqxmd.com/read/28894439/phenotypic-changes-exhibited-by-e-coli-cultured-in-space
#18
Luis Zea, Michael Larsen, Frederico Estante, Klaus Qvortrup, Ralf Moeller, Sílvia Dias de Oliveira, Louis Stodieck, David Klaus
Bacteria will accompany humans in our exploration of space, making it of importance to study their adaptation to the microgravity environment. To investigate potential phenotypic changes for bacteria grown in space, Escherichia coli was cultured onboard the International Space Station with matched controls on Earth. Samples were challenged with different concentrations of gentamicin sulfate to study the role of drug concentration on the dependent variables in the space environment. Analyses included assessments of final cell count, cell size, cell envelope thickness, cell ultrastructure, and culture morphology...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28894362/erratum-gentamicin-coating-of-nanotubular-anodized-titanium-implant-reduces-implant-related-osteomyelitis-and-enhances-bone-biocompatibility-in-rabbits-corrigendum
#19
(no author information available yet)
[This corrects the article on p. 5461 in vol. 12, PMID: 28814863.].
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28893782/aminoglycoside-concentrations-required-for-synergy-with-carbapenems-against-pseudomonas-aeruginosa-determined-via-mechanistic-studies-and-modeling
#20
Rajbharan Yadav, Jürgen B Bulitta, Elena K Schneider, Beom Soo Shin, Tony Velkov, Roger L Nation, Cornelia B Landersdorfer
This study aimed to systematically identify the aminoglycoside concentrations required for synergy with a carbapenem and characterize the permeabilizing effect of aminoglycosides on the outer membrane of Pseudomonas aeruginosa Monotherapies and combinations of four aminoglycosides and three carbapenems were studied against P. aeruginosa strain AH298-GFP in 48 h static-concentration time-kill studies (SCTK) (inoculum; 10(7.6) CFU/mL). The outer membrane permeabilizing effect of tobramycin alone and in combination with imipenem was characterized via electron micrographs, confocal imaging and the nitrocefin assay...
September 11, 2017: Antimicrobial Agents and Chemotherapy
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