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Type vi secretion system

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https://www.readbyqxmd.com/read/28934168/role-of-the-genes-of-type-vi-secretion-system-in-virulence-of-rice-bacterial-brown-stripe-pathogen-acidovorax-avenae-subsp-avenae-strain-rs-2
#1
Md Mahidul Islam Masum, Yingzi Yang, Bin Li, Ogunyemi Solabomi Olaitan, Jie Chen, Yang Zhang, Yushi Fang, Wen Qiu, Yanli Wang, Guochang Sun
The Type VI secretion system (T6SS) is a class of macromolecular machine that is required for the virulence of gram-negative bacteria. However, it is still not clear what the role of T6SS in the virulence of rice bacterial brown stripe pathogen Acidovorax avenae subsp. avenae (Aaa) is. The aim of the current study was to investigate the contribution of T6SS in Aaa strain RS2 virulence using insertional deletion mutation and complementation approaches. This strain produced weak virulence but contains a complete T6SS gene cluster based on a genome-wide analysis...
September 21, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28912123/type-vi-secretion-system-mix-effectors-carry-both-antibacterial-and-anti-eukaryotic-activities
#2
Ann Ray, Nika Schwartz, Marcela de Souza Santos, Junmei Zhang, Kim Orth, Dor Salomon
Most type VI secretion systems (T6SSs) described to date are protein delivery apparatuses that mediate bactericidal activities. Several T6SSs were also reported to mediate virulence activities, although only few anti-eukaryotic effectors have been described. Here, we identify three T6SSs in the marine bacterium Vibrio proteolyticus and show that T6SS1 mediates bactericidal activities under warm marine-like conditions. Using comparative proteomics, we find nine potential T6SS1 effectors, five of which belong to the polymorphic MIX-effector class...
September 14, 2017: EMBO Reports
https://www.readbyqxmd.com/read/28910638/the-landscape-of-type-vi-secretion-across-human-gut-microbiomes-reveals-its-role-in-community-composition
#3
Adrian J Verster, Benjamin D Ross, Matthew C Radey, Yiqiao Bao, Andrew L Goodman, Joseph D Mougous, Elhanan Borenstein
Although gut microbiome composition is well defined, the mechanisms underlying community assembly remain poorly understood. Bacteroidales possess three genetic architectures (GA1-3) of the type VI secretion system (T6SS), an effector delivery pathway that mediates interbacterial competition. Here we define the distribution and role of GA1-3 in the human gut using metagenomic analysis. We find that adult microbiomes harbor limited effector and cognate immunity genes, suggesting selection for compatibility at the species (GA1 and GA2) and strain (GA3) levels...
September 13, 2017: Cell Host & Microbe
https://www.readbyqxmd.com/read/28889297/contribution-of-cyclic-di-gmp-in-the-control-of-type-iii-and-type-vi-secretion-in-pseudomonas-aeruginosa
#4
Ronan R McCarthy, Martina Valentini, Alain Filloux
Bacteria produce toxins to enhance their competitiveness in the colonization of an environment as well as during an infection. The delivery of toxins into target cells is mediated by several types of secretion systems, among them our focus is Type III and Type VI Secretion Systems (T3SS and T6SS, respectively). A thorough methodology is provided detailing how to identify if cyclic di-GMP signaling plays a role in the P. aeruginosa toxin delivery mediated by T3SS or T6SS. This includes in vitro preparation of the samples for Western blot analysis aiming at detecting possible c-di-GMP-dependent T3SS/T6SS switch, as well as in vivo analysis using the model organism Galleria mellonella to demonstrate the ecological and pathogenic consequence of the switch between these two secretion systems...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28851366/a-widespread-family-of-polymorphic-toxins-encoded-by-temperate-phages
#5
Anne Jamet, Marie Touchon, Bruno Ribeiro-Gonçalves, João André Carriço, Alain Charbit, Xavier Nassif, Mario Ramirez, Eduardo P C Rocha
BACKGROUND: Polymorphic toxins (PTs) are multi-domain bacterial exotoxins belonging to distinct families that share common features in terms of domain organization. PTs are found in all major bacterial clades, including many toxic effectors of type V and type VI secretion systems. PTs modulate the dynamics of microbial communities by killing or inhibiting the growth of bacterial competitors lacking protective immunity proteins. RESULTS: In this work, we identified a novel widespread family of PTs, named MuF toxins, which were exclusively encoded within temperate phages and their prophages...
August 29, 2017: BMC Biology
https://www.readbyqxmd.com/read/28839029/genome-sequence-of-pantoea-ananatis-strain-amg-501-a-plant-growth-promoting-bacterium-isolated-from-rice-leaves-grown-in-paddies-of-southern-spain
#6
Esaú Megías, Fábio Bueno Reis Junior, Renan Augusto Ribeiro, Francisco Javier Ollero, Manuel Megías, Mariangela Hungria
Pantoea ananatis AMG 501 is a plant growth-promoting bacterium isolated from rice leaves. Its genome was estimated at 5,102,640 bp with 4,994 coding sequences, encompassing genes related to the metabolism of carbohydrates, to the synthesis of auxins, siderophores, and homoserine lactones, and to the type I, II, III, IV, and VI secretion systems.
August 24, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28830939/a-transcriptional-regulatory-mechanism-finely-tunes-the-firing-of-type-vi-secretion-system-in-response-to-bacterial-enemies
#7
Martina Lazzaro, Mario F Feldman, Eleonora García Véscovi
The ability to detect and measure danger from an environmental signal is paramount for bacteria to respond accordingly, deploying strategies that halt or counteract potential cellular injury and maximize survival chances. Type VI secretion systems (T6SSs) are complex bacterial contractile nanomachines able to target toxic effectors into neighboring bacteria competing for the same colonization niche. Previous studies support the concept that either T6SSs are constitutively active or they fire effectors in response to various stimuli, such as high bacterial density, cell-cell contact, nutrient depletion, or components from dead sibling cells...
August 22, 2017: MBio
https://www.readbyqxmd.com/read/28818038/role-of-the-type-vi-secretion-systems-during-disease-interactions-of-erwinia-amylovora-with-its-plant-host
#8
Tim Kamber, Joël F Pothier, Cosima Pelludat, Fabio Rezzonico, Brion Duffy, Theo H M Smits
BACKGROUND: Type VI secretion systems (T6SS) are widespread among Gram-negative bacteria and have a potential role as essential virulence factors or to maintain symbiotic interactions. Three T6SS gene clusters were identified in the genome of E. amylovora CFBP 1430, of which T6SS-1 and T6SS-3 represent complete T6SS machineries, while T6SS-2 is reduced in its gene content. RESULTS: To assess the contribution of T6SSs to virulence and potential transcriptomic changes of E...
August 17, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28817192/tssa-the-cap-protein-of-the-type-vi-secretion-system-tail
#9
REVIEW
Abdelrahim Zoued, Eric Durand, Yoann G Santin, Laure Journet, Alain Roussel, Christian Cambillau, Eric Cascales
The Type VI secretion system (T6SS) is a multiprotein and mosaic apparatus that delivers protein effectors into prokaryotic or eukaryotic cells. Recent data on the enteroaggregative Escherichia coli (EAEC) T6SS have provided evidence that the TssA protein is a key component during T6SS biogenesis. The T6SS comprises a trans-envelope complex that docks the baseplate, a cytoplasmic complex that represents the assembly platform for the tail. The T6SS tail is structurally, evolutionarily and functionally similar to the contractile tails of bacteriophages...
August 17, 2017: BioEssays: News and Reviews in Molecular, Cellular and Developmental Biology
https://www.readbyqxmd.com/read/28814272/identification-of-new-protein-coding-genes-with-a-potential-role-in-the-virulence-of-the-plant-pathogen-xanthomonas-euvesicatoria
#10
Ulrike Abendroth, Norman Adlung, Andreas Otto, Benjamin Grüneisen, Dörte Becher, Ulla Bonas
BACKGROUND: Bacteria of the genus Xanthomonas are economically important plant pathogens. Pathogenicity of Xanthomonas spp. depends on the type III-secretion system and additional virulence determinants. The number of sequenced Xanthomonas genomes increases rapidly, however, accurate annotation of these genomes is difficult, because it relies on gene prediction programs. In this study, we used a mass-spectrometry (MS)-based approach to identify the proteome of Xanthomonas euvesicatoria (Xe) strain 85-10 also known as X...
August 16, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28804481/the-type-vi-secretion-system-a-dynamic-system-for-bacterial-communication
#11
REVIEW
Mathias Gallique, Mathilde Bouteiller, Annabelle Merieau
Numerous studies in Gram-negative bacteria have focused on the Type VI Secretion Systems (T6SSs), Quorum Sensing (QS), and social behavior, such as in biofilms. These interconnected mechanisms are important for bacterial survival; T6SSs allow bacteria to battle other cells, QS is devoted to the perception of bacterial cell density, and biofilm formation is essentially controlled by QS. Here, we review data concerning T6SS dynamics and T6SS-QS cross-talk that suggest the existence of inter-bacterial communication via T6SSs...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28794863/recent-advances-in-understanding-pseudomonas-aeruginosa-as-a-pathogen
#12
REVIEW
Jens Klockgether, Burkhard Tümmler
The versatile and ubiquitous Pseudomonas aeruginosa is an opportunistic pathogen causing acute and chronic infections in predisposed human subjects. Here we review recent progress in understanding P. aeruginosa population biology and virulence, its cyclic di-GMP-mediated switches of lifestyle, and its interaction with the mammalian host as well as the role of the type III and type VI secretion systems in P. aeruginosa infection.
2017: F1000Research
https://www.readbyqxmd.com/read/28790987/comparative-analysis-of-the-flavobacterium-columnare-genomovar-i-and-ii-genomes
#13
Salih Kumru, Hasan C Tekedar, Nagihan Gulsoy, Geoffrey C Waldbieser, Mark L Lawrence, Attila Karsi
Columnaris disease caused by Gram-negative rod Flavobacterium columnare is one of the most common diseases of catfish. F. columnare is also a common problem in other cultured fish species worldwide. F. columnare has three major genomovars; we have sequenced a representative strain from genomovar I (ATCC 49512, which is avirulent in catfish) and genomovar II (94-081, which is highly pathogenic in catfish). Here, we present a comparative analysis of the two genomes. Interestingly, F. columnare ATCC 49512 and 94-081 meet criteria to be considered different species based on the Average Nucleotide Identity (90...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28777089/crystal-structure-of-the-putative-cytoplasmic-protein-stm0279-hcp2-from-salmonella-typhimurium
#14
Qing Peng Lin, Zeng Qiang Gao, Zhi Geng, Heng Zhang, Yu Hui Dong
STM0279 is a putative cytoplasmic protein from Salmonella typhimurium and was recently renamed haemolysin co-regulated protein 2 (Hcp2), with the neighbouring STM0276 being Hcp1. Both of them are encoded by the type VI secretion system (T6SS) of the Salmonella pathogenicity island 6 (SPI-6) locus and have high sequence identity. The Hcp proteins may function as a vital component of the T6SS nanotube and as a transporter and chaperone of diverse effectors from the bacterial T6SS. In this study, the crystal structure and the oligomeric state in solution of Hcp2 from S...
August 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/28760929/entry-intracellular-survival-and-multinucleated-giant-cell-forming-activity-of-burkholderia-pseudomallei-in-human-primary-phagocytic-and-non-phagocytic-cells
#15
Liam Whiteley, Teresa Meffert, Maria Haug, Christopher Weidenmaier, Verena Hopf, Katharina Bitschar, Birgit Schittek, Christian Kohler, Ivo Steinmetz, T Eoin West, Sandra Schwarz
The human pathogen Burkholderia pseudomallei and the related species Burkholderia thailandensis are facultative intracellular bacteria characterized by the ability to escape into the cytosol of the host cell and to stimulate the formation of multinucleated giant cells (MNGCs). MNGC formation is induced via an unknown mechanism by the bacterial type VI secretion system 5 (T6SS-5), which is an essential virulence factor in both species. Despite the vital role of the intracellular life cycle in the pathogenesis of the bacteria, the range of host cell types permissive for initiation and completion of the intracellular cycle is poorly defined...
July 31, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28758353/structural-and-saxs-analysis-of-tle5-tli5-complex-reveals-a-novel-inhibition-mechanism-of-h2-t6ss-in-pseudomonas-aeruginosa
#16
Xiao-Yun Yang, Zong-Qiang Li, Zeng-Qiang Gao, Wen-Jia Wang, Zhi Geng, Jian-Hua Xu, Zhun She, Yu-Hui Dong
Widely spread in gram-negative bacteria, the type VI secretion system (T6SS) secretes many effector-immunity protein pairs to help the bacteria compete against other prokaryotic rivals, and infect their eukaryotic hosts. Tle5 and Tle5B are two phospholipase effector protein secreted by T6SS of Pseudomonas aeruginosa. They can facilitate the bacterial internalization process into human epithelial cells by interacting with Akt protein of the PI3K-Akt signal pathway. Tli5 and PA5086-5088 are cognate immunity proteins of Tle5 and Tle5B, respectively...
July 30, 2017: Protein Science: a Publication of the Protein Society
https://www.readbyqxmd.com/read/28750654/different-therapeutic-effects-of-cells-derived-from-human-amniotic-membrane-on-premature-ovarian-aging-depend-on-distinct-cellular-biological-characteristics
#17
Chenyue Ding, Hong Li, Yun Wang, Fuxin Wang, Huihua Wu, Rulei Chen, Jinghuan Lv, Wei Wang, Boxian Huang
BACKGROUND: Many reports have shown that various kinds of stem cells have the ability to recover premature ovarian aging (POA) function. Transplantation of human amniotic epithelial cells (hAECs) improves ovarian function damaged by chemotherapy in a mice model. Understanding of how to evaluate the distinct effects of adult stem cells in curing POA and how to choose stem cells in clinical application is lacking. METHODS: To build a different degrees of POA model, mice were administered different doses of cyclophosphamide: light dose (70 mg/kg, 2 weeks), medium dose (70 mg/kg, 1 week; 120 mg/kg, 1 week), and high dose (120 mg/kg, 2 weeks)...
July 27, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28746878/the-type-vi-secretion-system-engages-a-redox-regulated-dual-functional-heme-transporter-for-zinc-acquisition
#18
Meiru Si, Yao Wang, Bing Zhang, Chao Zhao, Yiwen Kang, Haonan Bai, Dawei Wei, Lingfang Zhu, Lei Zhang, Tao G Dong, Xihui Shen
The type VI secretion system was recently reported to be involved in zinc acquisition, but the underlying mechanism remains unclear. Here, we report that Burkholderia thailandensis T6SS4 is involved in zinc acquisition via secretion of a zinc-scavenging protein, TseZ, that interacts with the outer membrane heme transporter HmuR. We find that HmuR is a redox-regulated dual-functional transporter that transports heme iron under normal conditions but zinc upon sensing extracellular oxidative stress, triggered by formation of an intramolecular disulfide bond...
July 25, 2017: Cell Reports
https://www.readbyqxmd.com/read/28745813/subversion-of-innate-immune-responses-by-francisella-involves-the-disruption-of-traf3-and-traf6-signaling-complexes
#19
Daniela Putzova, Swarupa Panda, Anetta Härtlova, Jiří Stulík, Nelson O Gekara
The success of pathogens depends on their ability to circumvent immune defenses. Francisella tularensis is one of the most infectious bacteria known. The remarkable virulence of Francisella is believed to be due to its capacity to evade or subvert the immune system, but how remains obscure. Here we show that Francisella triggers but concomitantly inhibits the Toll-like receptor (TLR), RIG-I-like receptor (RLR) and Cytoplasmic DNA (CDR) pathways. Francisella subverts these pathways at least in part by inhibiting K63-linked polyubiquitination and assembly of TRAF6 and TRAF3 complexes that control the transcriptional responses of PRRs...
July 26, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/28743812/horizontal-gene-transfer-of-functional-type-vi-killing-genes-by-natural-transformation
#20
Jacob Thomas, Samit S Watve, William C Ratcliff, Brian K Hammer
Horizontal gene transfer (HGT) can have profound effects on bacterial evolution by allowing individuals to rapidly acquire adaptive traits that shape their strategies for competition. One strategy for intermicrobial antagonism often used by Proteobacteria is the genetically encoded contact-dependent type VI secretion system (T6SS), a weapon used to kill heteroclonal neighbors by direct injection of toxic effectors. Here, we experimentally demonstrate that Vibrio cholerae can acquire new T6SS effector genes via horizontal transfer and utilize them to kill neighboring cells...
July 25, 2017: MBio
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