keyword
MENU ▼
Read by QxMD icon Read
search

Allelic exchange

keyword
https://www.readbyqxmd.com/read/28719641/inversion-of-the-imprinting-control-region-of-the-peg3-domain
#1
Joomyeong Kim, Hongzhi He, Hana Kim
The imprinting of the mouse Peg3 domain is controlled through a 4-kb genomic region encompassing the bidirectional promoter and 1st exons of Peg3 and Usp29. In the current study, this ICR was inverted to test its orientation dependency for the transcriptional and imprinting control of the Peg3 domain. The inversion resulted in the exchange of promoters and 1st exons between Peg3 and Usp29. Paternal transmission of this inversion caused 10-fold down-regulation of Peg3 and 2-fold up-regulation of Usp29 in neonatal heads, consistent with its original promoter strength in each direction...
2017: PloS One
https://www.readbyqxmd.com/read/28692664/a-functional-approach-to-understanding-the-role-of-nckx5-in-xenopus-pigmentation
#2
Ruth M Williams, Robert J Winkfein, Rebecca S Ginger, Martin R Green, Paul P Schnetkamp, Grant N Wheeler
NCKX5 is an ion exchanger expressed mostly in pigment cells; however, the functional role for this protein in melanogenesis is not clear. A variant allele of SLC24A5, the gene encoding NCKX5, has been shown to correlate with lighter skin pigmentation in humans, indicating a key role for SLC24A5 in determining human skin colour. SLC24A5 expression has been found to be elevated in melanoma. Knockdown analyses have shown SLC24A5 to be important for pigmentation, but to date the function of this ion exchanger in melanogenesis has not been fully established...
2017: PloS One
https://www.readbyqxmd.com/read/28687645/deficiency-of-rgpg-causes-major-defects-in-cell-division-and-biofilm-formation-and-deficiency-of-lcp-proteins-leads-to-accumulation-of-cell-wall-antigens-in-culture-medium-by-streptococcus-mutans
#3
Arpan De, Sumei Liao, Jacob P Bitoun, Randy Roth, Wandy L Beatty, Hui Wu, Zezhang T Wen
S. mutans is known to possess rhamnose-glucose polysaccharides (RGP), a major cell wall antigen. S. mutans strains deficient of rgpG, encoding the first enzyme of the RGP biosynthesis pathway, were constructed by allelic exchange. The rgpG deficiency had no effect on growth rate, but caused major defects in cell division and altered cell morphology. Unlike the coccoid wild-type, the rgpG mutant existed primarily in chains of swollen, squarish dividing cells. Deficiency of rgpG also causes significant reduction in biofilm formation (P<0...
July 7, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28684706/biomphalaria-glabrata-metallothionein-lacking-metal-specificity-of-the-protein-and-missing-gene-upregulation-suggest-metal-sequestration-by-exchange-instead-of-through-selective-binding
#4
Michael Niederwanger, Sara Calatayud, Oliver Zerbe, Sílvia Atrian, Ricard Albalat, Mercè Capdevila, Òscar Palacios, Reinhard Dallinger
The wild-type metallothionein (MT) of the freshwater snail Biomphalaria glabrata and a natural allelic mutant of it in which a lysine residue was replaced by an asparagine residue, were recombinantly expressed and analyzed for their metal-binding features with respect to Cd(2+), Zn(2+) and Cu⁺, applying spectroscopic and mass-spectrometric methods. In addition, the upregulation of the Biomphalaria glabrataMT gene was assessed by quantitative real-time detection PCR. The two recombinant proteins revealed to be very similar in most of their metal binding features...
July 6, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28677370/neuronal-vacuolation-and-spinocerebellar-degeneration-associated-with-altered-neurotransmission
#5
Aggeliki Giannakopoulou
<i>Inherited neurodegenerative disorders are debilitating diseases that occur across different species, such as the domestic dog (Canis lupus familiaris), and many are caused by mutations in the same genes as corresponding human conditions. In the present study, we report an inherited neurodegenerative condition, termed 'neuronal vacuolation and spinocerebellar degeneration' (NVSD) which affects neonatal or young dogs, mainly Rottweilers, which recently has been linked with the homozygosity for the RAB3GAP1:c...
2017: Folia Neuropathologica
https://www.readbyqxmd.com/read/28660361/establishment-of-a-markerless-gene-deletion-system-in-chromohalobacter-salexigens-dsm-3043
#6
Ya-Hui Shao, Li-Zhong Guo, Hao Yu, Bai-Suo Zhao, Wei-Dong Lu
Chromohalobacter salexigens DSM 3043 can grow over a wide range of salinity, which makes it as an excellent model organism for understanding the mechanism of prokaryotic osmoregulation. Functional analysis of C. salexigens genes is an essential way to reveal their roles in cellular osmoregulation. However, the lack of an effective markerless gene deletion system has prevented construction of multiple gene deletion mutants for the members in the genus. Here, we report the development of a markerless gene deletion system in C...
June 28, 2017: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/28659481/hepatitis-c-virus-strain-dependent-usage-of-apolipoprotein-e-modulates-assembly-efficiency-and-specific-infectivity-of-secreted-virions
#7
Romy Weller, Kathrin Hueging, Richard J P Brown, Daniel Todt, Sebastian Joecks, Florian W R Vondran, Thomas Pietschmann
Hepatitis C virus (HCV) is extraordinarily diverse and uses entry factors in a strain-specific manner. Virus particles associate with lipoproteins and Apolipoprotein E (ApoE) is critical for HCV assembly and infectivity. However, whether ApoE-dependency is common to all HCV genotypes remains unknown. Therefore, we compared the role of ApoE utilizing ten virus strains from genotypes 1 through 7. ApoA and ApoC also support HCV assembly, so they may contribute to virus production in a strain-dependent fashion...
June 28, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28658258/genome-scale-analysis-of-the-genes-that-contribute-to-burkholderia-pseudomallei-biofilm-formation-identifies-a-crucial-exopolysaccharide-biosynthesis-gene-cluster
#8
Grace I Borlee, Brooke A Plumley, Kevin H Martin, Nawarat Somprasong, Mihnea R Mangalea, M Nurul Islam, Mary N Burtnick, Paul J Brett, Ivo Steinmetz, David P AuCoin, John T Belisle, Dean C Crick, Herbert P Schweizer, Bradley R Borlee
Burkholderia pseudomallei, the causative agent of melioidosis, is an important public health threat due to limited therapeutic options for treatment. Efforts to improve therapeutics for B. pseudomallei infections are dependent on the need to understand the role of B. pseudomallei biofilm formation and its contribution to antibiotic tolerance and persistence as these are bacterial traits that prevent effective therapy. In order to reveal the genes that regulate and/or contribute to B. pseudomallei 1026b biofilm formation, we screened a sequence defined two-allele transposon library and identified 118 transposon insertion mutants that were deficient in biofilm formation...
June 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28655738/two-variants-in-slc24a5-are-associated-with-tiger-eye-iris-pigmentation-in-puerto-rican-paso-fino-horses
#9
Maura Mack, Elizabeth Kowalski, Robert Grahn, Dineli Bras, Maria Cecilia T Penedo, Rebecca Bellone
A unique eye color, called tiger-eye, segregates in the Puerto Rican Paso Fino Horse breed and is characterized by a bright yellow, amber, or orange iris. Pedigree analysis identified a simple autosomal recessive mode of inheritance for this trait. A genome-wide association study with 24 individuals identified a locus on ECA 1 reaching genome wide significance (Pcorrected =1.32 x 10(-5)). This ECA1 locus harbors the candidate gene, Solute Carrier Family 24 (Sodium/Potassium /Calcium Exchanger), Member 5 (SLC24A5), with known roles in pigmentation in humans, mice, and zebrafish...
June 27, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28650958/dynamics-and-impact-of-homologous-recombination-on-the-evolution-of-legionella-pneumophila
#10
Sophia David, Leonor Sánchez-Busó, Simon R Harris, Pekka Marttinen, Christophe Rusniok, Carmen Buchrieser, Timothy G Harrison, Julian Parkhill
Legionella pneumophila is an environmental bacterium and the causative agent of Legionnaires' disease. Previous genomic studies have shown that recombination accounts for a high proportion (>96%) of diversity within several major disease-associated sequence types (STs) of L. pneumophila. This suggests that recombination represents a potentially important force shaping adaptation and virulence. Despite this, little is known about the biological effects of recombination in L. pneumophila, particularly with regards to homologous recombination (whereby genes are replaced with alternative allelic variants)...
June 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28647612/evolution-of-human-apolipoprotein-e-apoe-isoforms-gene-structure-protein-function-and-interaction-with-dietary-factors
#11
REVIEW
Patricia Huebbe, Gerald Rimbach
Apolipoprotein E (APOE) is a member of the vertebrate protein family of exchangeable apolipoproteins that is characterized by amphipathic α-helices encoded by multiple nucleotide tandem repeats. Its equivalent in flying insects - apolipophorin-III - shares structural and functional commonalities with APOE, suggesting the possibility of an evolutionary relationship between the proteins. In contrast to all other known species, human APOE is functionally polymorphic and possesses three major allelic variants (ε4, ε3 and ε2)...
August 2017: Ageing Research Reviews
https://www.readbyqxmd.com/read/28640841/a-sorghum-sorghum-bicolor-mutant-with-altered-carbon-isotope-ratio
#12
Govinda Rizal, Shanta Karki, Vivek Thakur, Samart Wanchana, Hugo Alonso-Cantabrana, Jacque Dionora, John E Sheehy, Robert Furbank, Susanne von Caemmerer, William Paul Quick
Recent efforts to engineer C4 photosynthetic traits into C3 plants such as rice demand an understanding of the genetic elements that enable C4 plants to outperform C3 plants. As a part of the C4 Rice Consortium's efforts to identify genes needed to support C4 photosynthesis, EMS mutagenized sorghum populations were generated and screened to identify genes that cause a loss of C4 function. Stable carbon isotope ratio (δ13C) of leaf dry matter has been used to distinguishspecies with C3 and C4 photosynthetic pathways...
2017: PloS One
https://www.readbyqxmd.com/read/28629206/an-enhanced-vector-free-allele-exchange-vfae-mutagenesis-protocol-for-genome-editing-in-a-wide-range-of-bacterial-species
#13
Ahmed E Gomaa, Chen Zhang, Zhimin Yang, Liguo Shang, Shijie Jiang, Zhiping Deng, Yuhua Zhan, Wei Lu, Min Lin, Yongliang Yan
Vector-free allele exchange (VFAE) is a newly developed protocol for genome editing in Pseudomonas species. Although several parameters have been determined to optimize the procedures for obtaining a stable and high-frequency mutation, numerous false-positive clones still appear on the plate, which increases the difficulty of finding the desired mutants. It has also not been established whether this protocol can be used for genome editing in other bacterial species. In the current study, the protocol was modified to dramatically decrease the occurrence of false-positive colonies using Pseudomonas stutzeri A1501 as a model strain...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28613379/restoration-over-time-and-sustainability-of-schinus-terebinthifolius-raddi
#14
S V Álvares-Carvalho, R Silva-Mann, I B Gois, M F V Melo, A S Oliveira, R A Ferreira, L J Gomes
The success of recovery programs on degraded areas is dependent on the genetic material to be used, which should present heterozygosity and genetic diversity in native and recovered populations. This study was carried out to evaluate the model efficiency to enable the recovery of a degraded area of the Lower São Francisco, Sergipe, Brazil. The target species for this study was Schinus terebinthifolius Raddi. Three populations were analyzed, the recovered area, seed-tree source population, and native tree population border established to the recovered area...
May 31, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28611741/glycosylation-of-a-capsule-like-complex-clc-by-francisella-novicida-is-required-for-virulence-and-partial-protective-immunity-in-mice
#15
Kelly C Freudenberger Catanzaro, Anna E Champion, Nrusingh Mohapatra, Thomas Cecere, Thomas J Inzana
Francisella tularensis is a Gram-negative bacterium and the etiologic agent of tularemia. F. tularensis may appear encapsulated when examined by transmission electron microscopy (TEM), which is due to production of an extracellular capsule-like complex (CLC) when the bacterium is grown under specific environmental conditions. Deletion of two glycosylation genes in the live vaccine strain (LVS) results in loss of apparent CLC and attenuation of LVS in mice. In contrast, F. novicida, which is also highly virulent for mice, is reported to be non-encapsulated...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28607459/the-arf6-activator-efa6-psd3-confers-regional-specificity-and-modulates-ethanol-consumption-in-drosophila-and-humans
#16
D A Gonzalez, T Jia, J H Pinzón, S F Acevedo, S A Ojelade, B Xu, N Tay, S Desrivières, J L Hernandez, T Banaschewski, C Büchel, A L W Bokde, P J Conrod, H Flor, V Frouin, J Gallinat, H Garavan, P A Gowland, A Heinz, B Ittermann, M Lathrop, J-L Martinot, T Paus, M N Smolka, A R Rodan, G Schumann, A Rothenfluh
Ubiquitously expressed genes have been implicated in a variety of specific behaviors, including responses to ethanol. However, the mechanisms that confer this behavioral specificity have remained elusive. Previously, we showed that the ubiquitously expressed small GTPase Arf6 is required for normal ethanol-induced sedation in adult Drosophila. Here, we show that this behavioral response also requires Efa6, one of (at least) three Drosophila Arf6 guanine exchange factors. Ethanol-naive Arf6 and Efa6 mutants were sensitive to ethanol-induced sedation and lacked rapid tolerance upon re-exposure to ethanol, when compared with wild-type flies...
June 13, 2017: Molecular Psychiatry
https://www.readbyqxmd.com/read/28602864/nckx3-was-compensated-by-calcium-transporting-genes-and-bone-resorption-in-a-nckx3-ko-mouse-model
#17
Hyun Yang, Changhwan Ahn, Eun-Kyeong Shin, Ji-Sun Lee, Beum-Soo An, Eui-Bae Jeung
Gene knockout is the most powerful tool for determination of gene function or permanent modification of the phenotypic characteristics of an animal. Existing methods for gene disruption are limited by their efficiency, time required for completion and potential for confounding off-target effects. In this study, a rapid single-step approach to knockout of a targeted gene in mice using zinc-finger nucleases (ZFNs) was demonstrated for generation of mutant (knockout; KO) alleles. Specifically, ZFNs to target the sodium/calcium/potassium exchanger3 (NCKX3) gene in C57bl/6j were designed using the concept of this approach...
June 7, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28600642/characterization-and-comparison-of-genetic-variation-in-cotesia-flavipes-hymenoptera-braconidae-mass-reared-for-biological-pest-control-using-microsatellite-markers
#18
F C Freitas, A C Morales-Corrêa E Castro, N C C P Barbosa, O A Fernandes
The larval parasitoid Cotesia flavipes (Cameron) (Hymenoptera: Braconidae) is an important biological control agent of the sugarcane borer, Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae). This exotic parasitoid has been mass reared for field release since its introduction in the 1970s. Insects are exchanged between labs or introduced from the field, but without basic studies or criteria. Thus, the objective of this study was to evaluate the genetic variability of insects from six Brazilian States (São Paulo, Minas Gerais, Paraná, Goiás, Maranhão, and Alagoas) using microsatellites...
June 9, 2017: Neotropical Entomology
https://www.readbyqxmd.com/read/28597825/fungal-sex-the-basidiomycota
#19
Marco A Coelho, Guus Bakkeren, Sheng Sun, Michael E Hood, Tatiana Giraud
Fungi of the Basidiomycota, representing major pathogen lineages and mushroom-forming species, exhibit diverse means to achieve sexual reproduction, with particularly varied mechanisms to determine compatibilities of haploid mating partners. For species that require mating between distinct genotypes, discrimination is usually based on both the reciprocal exchange of diffusible mating pheromones, rather than sexes, and the interactions of homeodomain protein signals after cell fusion. Both compatibility factors must be heterozygous in the product of mating, and genetic linkage relationships of the mating pheromone/receptor and homeodomain genes largely determine the complex patterns of mating-type variation...
June 2017: Microbiology Spectrum
https://www.readbyqxmd.com/read/28596255/thrombotic-thrombocytopenic-purpura-associated-with-pegylated-interferon-alfa-2a-use-in-a-patient-with-polycythemia-vera
#20
Radhika Gangaraju, Soo J Kim, Jing-Fei Dong, Sabina Swierczek, Josef T Prchal
Pegylated interferon alfa-2a (pegIFNa) is being increasingly used for treatment of myeloproliferative neoplasms; however, its side effects, including autoimmune complications, are not unusual. We report on a 47-year-old woman with polycythemia vera (PV) treated with pegIFNa and in complete hematologic remission who developed thrombotic thrombocytopenic purpura (TTP). To our knowledge, thrombotic microangiopathy has been reported as a side effect of interferon (IFN) use in patients with hepatitis and chronic myeloid leukemia, but not in those with PV...
June 2017: Journal of the National Comprehensive Cancer Network: JNCCN
keyword
keyword
20135
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"