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klebsiella pneumoniae polysaccharide capsule

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https://www.readbyqxmd.com/read/29618504/kaptive-web-user-friendly-capsule-and-lipopolysaccharide-serotype-prediction-for-klebsiella-genomes
#1
Ryan R Wick, Eva Heinz, Kathryn E Holt, Kelly L Wyres
As whole genome sequencing becomes an established component of the microbiologist's toolbox, it is imperative that researchers, clinical microbiologists and public health professionals have access to genomic analysis tools for rapid extraction of epidemiologically and clinically relevant information. For the gram-negative hospital pathogens such as Klebsiella pneumoniae , initial efforts have focused on detection and surveillance of antimicrobial resistance genes and clones. However, with the resurgence of interest in alternative infection control strategies targeting Klebsiella surface polysaccharides, the ability to extract information about these antigens is increasingly important...
April 4, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29615497/novel-broadly-reactive-anticapsular-antibodies-against-carbapenem-resistant-klebsiella-pneumoniae-protect-from-infection
#2
Elizabeth Diago-Navarro, Michael P Motley, Gonzalo Ruiz-Peréz, Winnie Yu, Julianne Austin, Bruna M S Seco, Guozhi Xiao, Aniska Chikhalya, Peter H Seeberger, Bettina C Fries
Carbapenem-resistant (CR) sequence type 258 (ST258) Klebsiella pneumoniae has become an urgent health care threat, causing an increasing number of high-mortality infections. Its resistance to numerous antibiotics and threat to immunocompromised patients necessitate finding new therapies to combat these infections. Previous successes in the laboratory, as well as the conservation of capsular polysaccharide (CPS) among the members of the ST258 clone, suggest that monoclonal antibody (MAb) therapy targeting the outer polysaccharide capsule of K...
April 3, 2018: MBio
https://www.readbyqxmd.com/read/29535199/antibody-mediated-killing-of-carbapenem-resistant-st258-klebsiella-pneumoniae-by-human-neutrophils
#3
Scott D Kobayashi, Adeline R Porter, Brett Freedman, Ruchi Pandey, Liang Chen, Barry N Kreiswirth, Frank R DeLeo
Carbapenem-resistant Klebsiella pneumoniae is a problem worldwide. A carbapenem-resistant K. pneumoniae lineage classified as multilocus sequence type 258 (ST258) is prominent in the health care setting in many regions of the world, including the United States. ST258 strains can be resistant to virtually all clinically useful antibiotics; treatment of infections caused by these organisms is difficult, and mortality is high. As a step toward promoting development of new therapeutics for ST258 infections, we tested the ability of rabbit antibodies specific for ST258 capsule polysaccharide to enhance human serum bactericidal activity and promote phagocytosis and killing of these bacteria by human neutrophils...
March 13, 2018: MBio
https://www.readbyqxmd.com/read/29381692/rhinoscleroma-pathogenesis-the-type-k3-capsule-of-klebsiella-rhinoscleromatis-is-a-virulence-factor-not-involved-in-mikulicz-cells-formation
#4
Barbara Corelli, Ana S Almeida, Fabiane Sonego, Virginia Castiglia, Cindy Fevre, Sylvain Brisse, Philippe J Sansonetti, Régis Tournebize
Rhinoscleroma is a human specific chronic granulomatous infection of the nose and upper airways caused by the Gram-negative bacterium Klebsiella pneumoniae subsp. rhinoscleromatis. Although considered a rare disease, it is endemic in low-income countries where hygienic conditions are poor. A hallmark of this pathology is the appearance of atypical foamy monocytes called Mikulicz cells. However, the pathogenesis of rhinoscleroma remains poorly investigated. Capsule polysaccharide (CPS) is a prominent virulence factor in bacteria...
January 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29297821/isolation-identification-and-genome-analysis-of-lung-pathogenic-klebsiella-pneumoniae-lpkp-in-forest-musk-deer
#5
Wei Zhao, Qing Tian, Yan Luo, Yin Wang, Ze-Xiao Yang, Xue-Ping Yao, Jian-Guo Cheng, Xin Zhou, Wu-You Wang
Klebsiella pneumoniae is an important pathogen commonly associated with opportunistic infections. In this study, lung pathogenic K. pneumoniae (LPKP) was isolated and identified from suppurative pneumoniae in forest musk deer by conventional methods and by 16S ribosomal RNA sequence analysis. Median lethal dose and histopathologic analysis were used to demonstrate pathogenicity of the organism in mice. Furthermore, a draft genome of LPKP was sequenced, and its virulence genes were detected. One hundred and twenty-two virulence genes encoded determinant of capsule polysaccharide (CPS), lipopolysaccharide, fimbriae, outer membrane proteins, iron acquisition, and urease...
December 2017: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
https://www.readbyqxmd.com/read/29153063/-investigation-of-various-virulence-factors-of-klebsiella-pneumoniae-strains-isolated-from-nosocomial-infections
#6
Halit Kuş, Uğur Arslan, Hatice Türk Dağı, Duygu Fındık
Klebsiella pneumoniae is an opportunistic pathogen that commonly affects immunosuppressed patients and causes nosocomial infections. K.pneumoniae has a variety of virulence factors, especially capsule polysaccharide, hypermucoviscosity (HV), fimbriae, toxins and determinants for iron acquisition. The aim of this study was to detect the virulence factors in K.pneumoniae strains isolated from nosocomial infections in two years. Fifty three K.pneumoniae strains isolated from the samples of patients with nosocomial infections in the Medical Microbiology Laboratory of Selcuk University Faculty of Medicine Hospital between 2011 and 2013 were included in the study...
October 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28731269/biofilm-formation-and-antibiotic-resistance-in-klebsiella-pneumoniae-urinary-strains
#7
C Vuotto, F Longo, C Pascolini, G Donelli, M P Balice, M F Libori, V Tiracchia, A Salvia, P E Varaldo
AIMS: Multidrug-resistant Klebsiella pneumoniae has become a relevant healthcare-associated pathogen. Capsule, type 1 and 3 fimbriae (mrkA gene), type 2 quorum-sensing system (luxS), synthesis of D-galactan I (wbbM), LPS transport (wzm) and poly-beta-1,6-N-acetyl-D-glucosamine (pgaA) seem involved in K. pneumoniae biofilm. Nonenzymatic antibiotic resistance is related to nonexpression or mutation of porins (OmpK35 and OmpK36), and efflux pump (acrB) overexpression. The aim of this study was to analyse some virulence factors of K...
July 21, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28729207/first-two-cases-of-severe-multifocal-infections-caused-by-klebsiella-pneumoniae-in-switzerland-characterization-of-an-atypical-non-k1-k2-serotype-strain-causing-liver-abscess-and-endocarditis
#8
Baharak Babouee Flury, Valentina Donà, Niccolò Buetti, Hansjakob Furrer, Andrea Endimiani
BACKGROUND: We describe the first two multifocal invasive infections due to Klebsiella pneumoniae recently observed in Switzerland. METHODS: Phenotypic (MIC assays and string test) and molecular analyses (PCR/Sequencing for bla, virulence factor genes and whole genome sequencing for one strain) were performed to characterize the causative K. pneumoniae isolates. RESULTS: Both K. pneumoniae isolates (Kp1 and Kp2) were pan-susceptible to antibiotics and produced narrow-spectrum SHV β-lactamases...
September 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28728189/spontaneous-globe-rupture-due-to-rapidly-evolving-endogenous-hypermucoid-klebsiella-pneumoniae-endophthalmitis
#9
Edward H Wood, Matthew A Powers, Darius M Moshfeghi
In this retrospective report, the authors describe a rare case of spontaneous globe rupture from Klebsiella pneumoniae endophthalmitis in a middle-aged man with poorly controlled type 2 diabetes mellitus. There have been only four previously reported cases of spontaneous globe rupture from endophthalmitis. Out of the now five reported cases, all have been due to endogenous endophthalmitis, four have been due to gram-negative bacteria, and three have been due to K. pneumoniae. K. pneumoniae, especially the hypermucoid variant with a protective polysaccharide capsule, is an emerging pathogen with remarkable virulence...
July 1, 2017: Ophthalmic Surgery, Lasers & Imaging Retina
https://www.readbyqxmd.com/read/28676686/two-t7-like-bacteriophages-k5-2-and-k5-4-each-encodes-two-capsule-depolymerases-isolation-and-functional-characterization
#10
Pei-Fang Hsieh, Hsiao-Hsuan Lin, Tzu-Lung Lin, Yi-Yin Chen, Jin-Town Wang
Two Klebsiella bacteriophages K5-2 and K5-4, which are able to infect and grow on either capsular types K30/K69 and K5 or K8 and K5 of Klebsiella strains, were isolated and characterized. Each phage contained two open reading frames (ORFs), which encoded two putative capsule depolymerases, respectively. The first ORF encoded tail fiber proteins, which have K30/K69 depolymerase and K8 depolymerase activities. The second ORF encoded hypothetical proteins, which are almost identical in amino acid sequences, and have K5 depolymerase activity...
July 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28486697/evolution-and-dissemination-of-the-klebsiella-pneumoniae-clonal-group-258-throughout-israeli-post-acute-care-hospitals-2008-13
#11
Amos Adler, Ziv Lifshitz, Michal Gordon, Debbie Ben-David, Efrat Khabra, Samira Masarwa, Orit Zion, Mitchell J Schwaber, Yehuda Carmeli
Objectives: The KPC-producing Klebsiella pneumoniae (KPC-KP) clonal group (CG) 258 has disseminated throughout Israeli post-acute care hospitals (PACHs). The objectives of the study were (i) to describe the evolution and (ii) to understand the dissemination modes of CG 258 in the PACH system in Israel. Methods: KPC-KP surveillance cultures isolates were collected in Israeli PACHs in three national point-prevalence surveys: 2008, 2011 and 2013. CG 258 was identified by pilv-l PCR...
August 1, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28437231/capsular-polysaccharide-types-and-virulence-related-traits-of-epidemic-kpc-producing-klebsiella-pneumoniae-isolates-in-a-chinese-university-hospital
#12
Yang Liu, Pan-Pan Liu, Lian-Hui Wang, Dan-Dan Wei, La-Gen Wan, Wei Zhang
Klebsiella pneumoniae is an important human pathogen associated with a variety of diseases and the prevalence of blaKPC carrying K. pneumoniae (KPC-Kp) is rapidly increasing. Capsule is an important virulence factor in K. pneumoniae. In this study, we determined to first systematically characterize capsular polysaccharide (CPS) and virulence traits in KPC-Kp strains. A total of 56 KPC-Kp isolates were recovered from clinical samples in a Chinese hospital, which were assigned to clonal lineages by multilocus sequence typing (MLST)...
October 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28348868/the-diversity-of-klebsiella-pneumoniae-surface-polysaccharides
#13
Rainer Follador, Eva Heinz, Kelly L Wyres, Matthew J Ellington, Michael Kowarik, Kathryn E Holt, Nicholas R Thomson
Klebsiella pneumoniae is considered an urgent health concern due to the emergence of multi-drug-resistant strains for which vaccination offers a potential remedy. Vaccines based on surface polysaccharides are highly promising but need to address the high diversity of surface-exposed polysaccharides, synthesized as O-antigens (lipopolysaccharide, LPS) and K-antigens (capsule polysaccharide, CPS), present in K. pneumoniae. We present a comprehensive and clinically relevant study of the diversity of O- and K-antigen biosynthesis gene clusters across a global collection of over 500 K...
August 2016: Microbial Genomics
https://www.readbyqxmd.com/read/28348840/identification-of-klebsiella-capsule-synthesis-loci-from-whole-genome-data
#14
Kelly L Wyres, Ryan R Wick, Claire Gorrie, Adam Jenney, Rainer Follador, Nicholas R Thomson, Kathryn E Holt
Klebsiella pneumoniae is a growing cause of healthcare-associated infections for which multi-drug resistance is a concern. Its polysaccharide capsule is a major virulence determinant and epidemiological marker. However, little is known about capsule epidemiology since serological typing is not widely accessible and many isolates are serologically non-typeable. Molecular typing techniques provide useful insights, but existing methods fail to take full advantage of the information in whole genome sequences. We investigated the diversity of the capsule synthesis loci (K-loci) among 2503 K...
December 2016: Microbial Genomics
https://www.readbyqxmd.com/read/28081233/differences-in-inflammatory-response-induced-by-two-representatives-of-clades-of-the-pandemic-st258-klebsiella-pneumoniae-clonal-lineage-producing-kpc-type-carbapenemases
#15
Giuseppe Castronovo, Ann Maria Clemente, Alberto Antonelli, Marco Maria D'Andrea, Michele Tanturli, Eloisa Perissi, Sara Paccosi, Astrid Parenti, Federico Cozzolino, Gian Maria Rossolini, Maria Gabriella Torcia
ST258-K. pneumoniae (ST258-KP) strains, the most widespread multidrug-resistant hospital-acquired pathogens, belong to at least two clades differing in a 215 Kb genomic region that includes the cluster of capsule genes. To investigate the effects of the different capsular phenotype on host-pathogen interactions, we studied representatives of ST258-KP clades, KKBO-1 and KK207-1, for their ability to activate monocytes and myeloid dendritic cells from human immune competent hosts. The two ST258-KP strains strongly induced the production of inflammatory cytokines...
2017: PloS One
https://www.readbyqxmd.com/read/27795303/antibody-based-immunotherapy-to-treat-and-prevent-infection-with-hypervirulent-klebsiella-pneumoniae
#16
Elizabeth Diago-Navarro, Isabel Calatayud-Baselga, Donglei Sun, Camille Khairallah, Inderjit Mann, Amaia Ulacia-Hernando, Brian Sheridan, Meiqing Shi, Bettina C Fries
Hypervirulent Klebsiella pneumoniae (hvKp) strains are predicted to become a major threat in Asia if antibiotic resistance continues to spread. Anticapsular antibodies (Abs) were developed because disseminated infections caused by hvKp are associated with significant morbidity and mortality, even with antibiotic-sensitive strains. K1-serotype polysaccharide capsules (K1-CPS) are expressed by the majority of hvKp strains. In this study, K1-CPS-specific IgG Abs were generated by conjugation of K1-CPS to immunogenic anthrax protective antigen (PA) protein...
January 2017: Clinical and Vaccine Immunology: CVI
https://www.readbyqxmd.com/read/27752727/a-nanomechanical-study-of-the-effects-of-colistin-on-the-klebsiella-pneumoniae-aj218-capsule
#17
Anna Mularski, Jonathan Wilksch, Eric Hanssen, Jian Li, Takehiro Tomita, Sacha James Pidot, Tim Stinear, Frances Separovic, Dick Strugnell
Atomic force microscopy measurements of capsule thickness revealed that that the wild-type Klebsiella pneumoniae AJ218 capsular polysaccharides were rearranged by exposure to colistin. The increase in capsule thickness measured near minimum inhibitory/bactericidal concentration (MIC/MBC) is consistent with the idea that colistin displaces the divalent cations that cross-bridge adjacent lipopolysaccharide (LPS) molecules through the capsule network. Cryo-electron microscopy demonstrated that the measured capsule thickness at near MIC/MBC of 1...
May 2017: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/27715471/the-contribution-of-capsule-polysaccharide-genes-to-virulence-of-klebsiella-pneumoniae
#18
Kwan Soo Ko
No abstract text is available yet for this article.
July 4, 2017: Virulence
https://www.readbyqxmd.com/read/27683817/cd36-provides-host-protection-against-klebsiella-pneumoniae-intrapulmonary-infection-by-enhancing-lipopolysaccharide-responsiveness-and-macrophage-phagocytosis
#19
Tolani F Olonisakin, Huihua Li, Zeyu Xiong, Elizabeth J K Kochman, Minting Yu, Yanyan Qu, Mei Hulver, Jay K Kolls, Claudette St Croix, Yohei Doi, Minh-Hong Nguyen, Robert M Q Shanks, Rama K Mallampalli, Valerian E Kagan, Anuradha Ray, Roy L Silverstein, Prabir Ray, Janet S Lee
Klebsiella pneumoniae remains an important cause of intrapulmonary infection and invasive disease worldwide. K. pneumoniae can evade serum killing and phagocytosis primarily through the expression of a polysaccharide capsule, but its pathogenicity is also influenced by host factors. We examined whether CD36, a scavenger receptor that recognizes pathogen and modified self ligands, is a host determinant of K. pneumoniae pathogenicity. Despite differences in serum sensitivity and virulence of 3 distinct K. pneumoniae (hypermucoviscous K1, research K2, and carbapenemase-producing ST258) strains, the absence of CD36 significantly increased host susceptibility to acute intrapulmonary infection by K...
December 15, 2016: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/27565716/effect-in-virulence-of-switching-conserved-homologous-capsular-polysaccharide-genes-from-klebsiella-pneumoniae-serotype-k1-into-k20
#20
Chii-Lan Lin, Fei-Hsu Chen, Li-Yueh Huang, Jen-Chang Chang, Jiun-Han Chen, Yu-Kuo Tsai, Feng-Yee Chang, Jung-Chung Lin, L Kristopher Siu
The capsular polysaccharides in different serotypes of Klebsiella pneumoniae (KP) coded by the (CPS) gene cluster are characterized by a conserved and a hyper-variable region. We performed a virulence study by switching genes in the highly conserved region of the CPS cluster between strains. Six genes in the CPS conserved region in serotype K20, including galF, acidPPc, wzi, wza, wzb and wzc, were knocked out and replaced by the homologous genes from serotype K1. Compared to the parental K20 strain, the mutants showed a decline in lethality (LD50) in mice from 10-fold to > 10(5)-fold and were categorized in terms of the effect on virulence as low (L) for galF and acidPPC, moderate (M) for wzi, and high (H) for wza, wzb and wzc...
July 4, 2017: Virulence
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