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MGH 78578

Graham G Willsey, Sebastian Ventrone, Kristin C Schutz, Aaron M Wallace, John W Ribis, Benjamin T Suratt, Matthew J Wargo
The interactions between K lebsiella pneumoniae and the host environment at the site of infection largely unknown. Pulmonary surfactant serves as an initial point of contact for inhaled bacteria entering the lung and is thought to contain molecular cues that aid colonization and pathogenesis. To gain insight into this ecological transition, we characterized the transcriptional response of K. pneumoniae MGH 78578 to purified pulmonary surfactant. This work revealed changes within the K. pneumoniae transcriptome that likely contribute to host colonization, adaptation, and virulence in vivo Notable transcripts expressed under these conditions include genes involved in capsule synthesis, LPS modification, antibiotic resistance, biofilm formation, and metabolism...
April 30, 2018: Infection and Immunity
Yu-Tze Horng, Chi-Jen Wang, Wen-Ting Chung, Huei-Jen Chao, Yih-Yuan Chen, Po-Chi Soo
BACKGROUND/PURPOSE: Klebsiella pneumoniae is one of the leading causes of device-related infections (DRIs), which are associated with attachment of bacteria to these devices to form a biofilm. The latter is composed of not only bacteria but also extracellular polymeric substances (EPSes) consisting of extracellular DNAs, polysaccharides, and other macromolecules. The phosphoenolpyruvate (PEP):carbohydrate phosphotransferase system (PTS) regulates diverse processes of bacterial physiology...
April 2018: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
Christopher S Henry, Ella Rotman, Wyndham W Lathem, Keith E J Tyo, Alan R Hauser, Mark J Mandel
Klebsiella pneumoniae has a reputation for causing a wide range of infectious conditions, with numerous highly virulent and antibiotic-resistant strains. Metabolic models have the potential to provide insights into the growth behavior, nutrient requirements, essential genes, and candidate drug targets in these strains. Here we develop a metabolic model for KPPR1, a highly virulent strain of K. pneumoniae. We apply a combination of Biolog phenotype data and fitness data to validate and refine our KPPR1 model...
February 15, 2017: Journal of Infectious Diseases
Arianna I Celis, Zachary Geeraerts, David Ngmenterebo, Melodie M Machovina, Richard C Kurker, Kumar Rajakumar, Anabella Ivancich, Kenton R Rodgers, Gudrun S Lukat-Rodgers, Jennifer L DuBois
Chlorite dismutases (Clds) convert chlorite to O2 and Cl(-), stabilizing heme in the presence of strong oxidants and forming the O═O bond with high efficiency. The enzyme from the pathogen Klebsiella pneumoniae (KpCld) represents a subfamily of Clds that share most of their active site structure with efficient O2-producing Clds, even though they have a truncated monomeric structure, exist as a dimer rather than a pentamer, and come from Gram-negative bacteria without a known need to degrade chlorite. We hypothesized that KpCld, like others in its subfamily, should be able to make O2 and may serve an in vivo antioxidant function...
January 20, 2015: Biochemistry
Ramy A Fodah, Jacob B Scott, Hok-Hei Tam, Pearlly Yan, Tia L Pfeffer, Ralf Bundschuh, Jonathan M Warawa
Klebsiella pneumoniae is a bacterial pathogen of worldwide importance and a significant contributor to multiple disease presentations associated with both nosocomial and community acquired disease. ATCC 43816 is a well-studied K. pneumoniae strain which is capable of causing an acute respiratory disease in surrogate animal models. In this study, we performed sequencing of the ATCC 43816 genome to support future efforts characterizing genetic elements required for disease. Furthermore, we performed comparative genetic analyses to the previously sequenced genomes from NTUH-K2044 and MGH 78578 to gain an understanding of the conservation of known virulence determinants amongst the three strains...
2014: PloS One
Yohei Doi, Tracy H Hazen, Matthew Boitano, Yu-Chih Tsai, Tyson A Clark, Jonas Korlach, David A Rasko
The whole-genome sequence of a carbapenem-resistant Klebsiella pneumoniae strain, PittNDM01, which coproduces NDM-1 and OXA-232 carbapenemases, was determined in this study. The use of single-molecule, real-time (SMRT) sequencing provided a closed genome in a single sequencing run. K. pneumoniae PittNDM01 has a single chromosome of 5,348,284 bp and four plasmids: pPKPN1 (283,371 bp), pPKPN2 (103,694 bp), pPKPN3 (70,814 bp), and pPKPN4 (6,141 bp). The contents of the chromosome were similar to that of the K...
October 2014: Antimicrobial Agents and Chemotherapy
Boon Aun Teh, Sy Bing Choi, Nasihah Musa, Few Ling Ling, See Too Wei Cun, Abu Bakar Salleh, Nazalan Najimudin, Habibah A Wahab, Yahaya M Normi
BACKGROUND: Klebsiella pneumoniae plays a major role in causing nosocomial infection in immunocompromised patients. Medical inflictions by the pathogen can range from respiratory and urinary tract infections, septicemia and primarily, pneumonia. As more K. pneumoniae strains are becoming highly resistant to various antibiotics, treatment of this bacterium has been rendered more difficult. This situation, as a consequence, poses a threat to public health. Hence, identification of possible novel drug targets against this opportunistic pathogen need to be undertaken...
2014: BMC Structural Biology
Pablo Ivan Pereira Ramos, Renata Christina Picão, Luiz Gonzaga Paula de Almeida, Nicholas Costa B Lima, Raquel Girardello, Ana Carolina P Vivan, Danilo E Xavier, Fernando G Barcellos, Marsileni Pelisson, Eliana C Vespero, Claudine Médigue, Ana Tereza Ribeiro de Vasconcelos, Ana Cristina Gales, Marisa Fabiana Nicolás
BACKGROUND: Klebsiella pneumoniae is an important opportunistic pathogen associated with nosocomial and community-acquired infections. A wide repertoire of virulence and antimicrobial resistance genes is present in K. pneumoniae genomes, which can constitute extra challenges in the treatment of infections caused by some strains. K. pneumoniae Kp13 is a multidrug-resistant strain responsible for causing a large nosocomial outbreak in a teaching hospital located in Southern Brazil. Kp13 produces K...
2014: BMC Genomics
Tzu-Wen Huang, Irene Lam, Hwan-You Chang, Shih-Feng Tsai, Bernhard O Palsson, Pep Charusanti
BACKGROUND: Klebsiella pneumoniae is a leading cause of hospital-acquired urinary tract infections and pneumonia worldwide, and is responsible for many cases of pyogenic liver abscess among diabetic patients in Asia. A defining characteristic of this pathogen is the presence of a thick, exterior capsule that has been reported to play a role in biofilm formation and to protect the organism from threats such antibiotics and host immune challenge. FINDINGS: We constructed two knockout mutants of K...
2014: BMC Research Notes
Kai Wei Kelvin Lee, Krithika Arumugam, Rikky Wenang Purbojati, Qi Xiang Martin Tay, Rohan Benjamin Hugh Williams, Staffan Kjelleberg, Scott A Rice
Klebsiella pneumoniae is ubiquitous in the environment and is a member of a three-species biofilm model. We compared the genome sequence of an environmental isolate, K. pneumoniae strain KP-1, to those of two clinical strains (NTUH-K2044 and MGH 78578). KP-1 possesses strain-specific prophage sequences that distinguish it from the clinical strains.
2013: Genome Announcements
M H Zulkifli, L K Teh, L S Lee, Z A Zakaria, M Z Salleh
Klebsiella pneumoniae PR04 was isolated from a patient hospitalized in Malaysia. The draft genome sequence of K. pneumoniae PR04 shows differences compared to the reference sequences of K. pneumoniae strains MGH 78578 and NTUH-K2044 in terms of their genomic structures.
2013: Genome Announcements
Shyamasree De Majumdar, Mark Veleba, Sarah Finn, Séamus Fanning, Thamarai Schneiders
RarA is an AraC-type regulator in Klebsiella pneumoniae, which, when overexpressed, confers a low-level multidrug-resistant (MDR) phenotype linked to the upregulation of both the acrAB and oqxAB efflux genes. Increased rarA expression has also been shown to be integral in the development of tigecycline resistance in the absence of ramA in K. pneumoniae. Given its phenotypic role in MDR, microarray analyses were performed to determine the RarA regulon. Transcriptome analysis was undertaken using strains Ecl8ΔrarA/pACrarA-2 (rarA-expressing construct) and Ecl8ΔrarA/pACYC184 (vector-only control) using bespoke microarray slides consisting of probes derived from the genomic sequences of K...
April 2013: Antimicrobial Agents and Chemotherapy
Joo-Hyun Seo, Jay Sung-Joong Hong, Donghyuk Kim, Byung-Kwan Cho, Tzu-Wen Huang, Shih-Feng Tsai, Bernhard O Palsson, Pep Charusanti
BACKGROUND: The increasing number of infections caused by strains of Klebsiella pneumoniae that are resistant to multiple antibiotics has developed into a major medical problem worldwide. The development of next-generation sequencing technologies now permits rapid sequencing of many K. pneumoniae isolates, but sequence information alone does not provide important structural and operational information for its genome. RESULTS: Here we take a systems biology approach to annotate the K...
2012: BMC Genomics
Soojin Lee, Borim Kim, Minkyu Oh, Youngrok Kim, Jinwon Lee
Meso-secondary alcohol dehydrogenases (meso-SADH) from Klebsiella oxytoca KCTC1686 and Klebsiella pneumoniae KCTC2242 were codon optimized and expressed in Escherichia coli W3110. The published gene data of K. pneumoniae NTUH-K2044 (NCBI accession number AP006725), K. pneumoniae 342 (NCBI accession number CP000964), and K. pneumoniae MGH 78578 (NCBI accession number CP000647), were compared with the meso-SADH sequences of each strain, respectively. Codon-optimized meso-SADH enzymes of K. oxytoca and K. pneumoniae showed approximately twofold to fivefold increased enzyme activities for acetoin reduction over native enzymes...
July 2013: Bioprocess and Biosystems Engineering
Diana P Cruz, Mónica G Huertas, Marcela Lozano, Lina Zárate, María Mercedes Zambrano
BACKGROUND: Klebsiella pneumoniae can be found in environmental habitats as well as in hospital settings where it is commonly associated with nosocomial infections. One of the factors that contribute to virulence is its capacity to form biofilms on diverse biotic and abiotic surfaces. The second messenger Bis-(3'-5')-cyclic dimeric GMP (c-di-GMP) is a ubiquitous signal in bacteria that controls biofilm formation as well as several other cellular processes. The cellular levels of this messenger are controlled by c-di-GMP synthesis and degradation catalyzed by diguanylate cyclase (DGC) and phophodiesterase (PDE) enzymes, respectively...
2012: BMC Microbiology
Mark Veleba, Paul G Higgins, Gerardo Gonzalez, Harald Seifert, Thamarai Schneiders
Transcriptional regulators, such as SoxS, RamA, MarA, and Rob, which upregulate the AcrAB efflux pump, have been shown to be associated with multidrug resistance in clinically relevant Gram-negative bacteria. In addition to the multidrug resistance phenotype, these regulators have also been shown to play a role in the cellular metabolism and possibly the virulence potential of microbial cells. As such, the increased expression of these proteins is likely to cause pleiotropic phenotypes. Klebsiella pneumoniae is a major nosocomial pathogen which can express the SoxS, MarA, Rob, and RamA proteins, and the accompanying paper shows that the increased transcription of ramA is associated with tigecycline resistance (M...
August 2012: Antimicrobial Agents and Chemotherapy
Mun Teng Wong, Sy Bing Choi, Chee Sian Kuan, Siang Ling Chua, Chiat Han Chang, Yahaya Mohd Normi, Wei Cun See Too, Habibah A Wahab, Ling Ling Few
Klebsiella pneumoniae is a Gram-negative, cylindrical rod shaped opportunistic pathogen that is found in the environment as well as existing as a normal flora in mammalian mucosal surfaces such as the mouth, skin, and intestines. Clinically it is the most important member of the family of Enterobacteriaceae that causes neonatal sepsis and nosocomial infections. In this work, a combination of protein sequence analysis, structural modeling and molecular docking simulation approaches were employed to provide an understanding of the possible functions and characteristics of a hypothetical protein (KPN_02809) from K...
2012: International Journal of Molecular Sciences
Chee Sian Kuan, Mun Teng Wong, Sy Bing Choi, Ching Ching Chang, Yoke Hiang Yee, Habibah A Wahab, Yahaya Mohd Normi, Wei Cun See Too, Ling Ling Few
Klebsiella pneumoniae causes neonatal sepsis and nosocomial infections. One of the strains, K. pneumoniae MGH 78578, shows high level of resistance to multiple microbial agents. In this study, domain family, amino acid sequence and topology analyses were performed on one of its hypothetical protein, YggG (KPN_03358). Structural bioinformatics approaches were used to predict the structure and functionality of YggG protein. The open reading frame (ORF) of yggG, which was a putative metalloprotease gene, was also cloned, expressed and characterized...
2011: International Journal of Molecular Sciences
Yu-Chieh Liao, Tzu-Wen Huang, Feng-Chi Chen, Pep Charusanti, Jay S J Hong, Hwan-You Chang, Shih-Feng Tsai, Bernhard O Palsson, Chao A Hsiung
Klebsiella pneumoniae is a Gram-negative bacterium of the family Enterobacteriaceae that possesses diverse metabolic capabilities: many strains are leading causes of hospital-acquired infections that are often refractory to multiple antibiotics, yet other strains are metabolically engineered and used for production of commercially valuable chemicals. To study its metabolism, we constructed a genome-scale metabolic model (iYL1228) for strain MGH 78578, experimentally determined its biomass composition, experimentally determined its ability to grow on a broad range of carbon, nitrogen, phosphorus and sulfur sources, and assessed the ability of the model to accurately simulate growth versus no growth on these substrates...
April 2011: Journal of Bacteriology
Debanu Das, Piotr Kozbial, Gye Won Han, Dennis Carlton, Lukasz Jaroszewski, Polat Abdubek, Tamara Astakhova, Herbert L Axelrod, Constantina Bakolitsa, Connie Chen, Hsiu Ju Chiu, Michelle Chiu, Thomas Clayton, Marc C Deller, Lian Duan, Kyle Ellrott, Marc André Elsliger, Dustin Ernst, Carol L Farr, Julie Feuerhelm, Anna Grzechnik, Joanna C Grant, Kevin K Jin, Hope A Johnson, Heath E Klock, Mark W Knuth, S Sri Krishna, Abhinav Kumar, David Marciano, Daniel McMullan, Mitchell D Miller, Andrew T Morse, Edward Nigoghossian, Amanda Nopakun, Linda Okach, Silvya Oommachen, Jessica Paulsen, Christina Puckett, Ron Reyes, Christopher L Rife, Natasha Sefcovic, Henry J Tien, Christine B Trame, Henry van den Bedem, Dana Weekes, Tiffany Wooten, Qingping Xu, Keith O Hodgson, John Wooley, Ashley M Deacon, Adam Godzik, Scott A Lesley, Ian A Wilson
KPN03535 (gi|152972051) is a putative lipoprotein of unknown function that is secreted by Klebsiella pneumoniae MGH 78578. The crystal structure reveals that despite a lack of any detectable sequence similarity to known structures, it is a novel variant of the OB-fold and structurally similar to the bacterial Cpx-pathway protein NlpE, single-stranded DNA-binding (SSB) proteins and toxins. K. pneumoniae MGH 78578 forms part of the normal human skin, mouth and gut flora and is an opportunistic pathogen that is linked to about 8% of all hospital-acquired infections in the USA...
October 1, 2010: Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
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