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Micro-rna

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https://www.readbyqxmd.com/read/28642484/mir-100-5p-inhibition-induces-apoptosis-in-dormant-prostate-cancer-cells-and-prevents-the-emergence-of-castration-resistant-prostate-cancer
#1
Noushin Nabavi, Nur Ridzwan Nur Saidy, Erik Venalainen, Anne Haegert, Abhijit Parolia, Hui Xue, Yuwei Wang, Rebecca Wu, Xin Dong, Colin Collins, Francesco Crea, Yuzhuo Wang
Carcinoma of the prostate is the most common cancer in men. Treatment of aggressive prostate cancer involves a regiment of radical prostectomy, radiation therapy, chemotherapy and hormonal therapy. Despite significant improvements in the last decade, the treatment of prostate cancer remains unsatisfactory, because a significant fraction of prostate cancers develop resistance to multiple treatments and become incurable. This prompts an urgent need to investigate the molecular mechanisms underlying the evolution of therapy-induced resistance of prostate cancer either in the form of castration-resistant prostate cancer (CRPC) or transdifferentiated neuroendocrine prostate cancer (NEPC)...
June 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28628915/mir-495-promotes-senescence-of-mesenchymal-stem-cells-by-targeting-bmi-1
#2
Xiujun Li, Yuxian Song, Dan Liu, Jiaojiao Zhao, Jingjing Xu, Jing Ren, Yali Hu, Zhiqun Wang, Yayi Hou, Guangfeng Zhao
BACKGROUND/AIMS: Mesenchymal stem cells (MSCs) play an important role in regulating angiogenesis and immune balance. Abnormal proliferation and function of MSCs were reported at maternal fetal interface in patients with pre-eclampsia (PE). Micro-RNA-495 was known to be upregulated in the MSCs derived from patients with PE. However, it is not clear whether the up-regulated miR-495 is related to the pathogenesis of PE. METHODS: We analyzed the expression of miR-495 in MSCs and umbilical cords derived from healthy pregnancies (NC) and PE, then we upregulated or downregulated the expression of miR-495 in MSCs derived from NC and tested the proliferation, apoptosis, migration, invasion, tube formation and senescence...
June 19, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28623160/basic-concepts-in-molecular-biology-related-to-genetics-and-epigenetics
#3
Dolores Corella, Jose M Ordovas
The observation that "one size does not fit all" for the prevention and treatment of cardiovascular disease, among other diseases, has driven the concept of precision medicine. The goal of precision medicine is to provide the best-targeted interventions tailored to an individual's genome. The human genome is composed of billions of sequence arrangements containing a code that controls how genes are expressed. This code depends on other nonstatic regulators that surround the DNA and constitute the epigenome...
June 13, 2017: Revista Española de Cardiología
https://www.readbyqxmd.com/read/28620300/novel-anti-fibrotic-therapies
#4
REVIEW
Benita L McVicker, Robert G Bennett
Fibrosis is a major player in cardiovascular disease, both as a contributor to the development of disease, as well as a post-injury response that drives progression. Despite the identification of many mechanisms responsible for cardiovascular fibrosis, to date no treatments have emerged that have effectively reduced the excess deposition of extracellular matrix associated with fibrotic conditions. Novel treatments have recently been identified that hold promise as potential therapeutic agents for cardiovascular diseases associated with fibrosis, as well as other fibrotic conditions...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28617420/mirna-mediated-post-transcriptional-silencing-of-transgenes-leads-to-increased-adeno-associated-viral-vector-yield-and-targeting-specificity
#5
C A Reid, S L Boye, W W Hauswirth, D M Lipinski
The production of high titer recombinant adeno-associated virus (rAAV) vector is essential for treatment of genetic diseases affecting the retina and choroid, where anatomical constraints may limit injectable volumes. Problematically, cytotoxicity arising from over-expression of the transgene during vector production frequently leads to a reduction in vector yield. Herein, we evaluate the use of micro-RNA (miRNA) mediated silencing to limit over-expression of cytotoxic transgenes during packaging as a method of increasing vector yield...
June 15, 2017: Gene Therapy
https://www.readbyqxmd.com/read/28600885/immunolocalization-of-tar-dna-binding-protein-of-43%C3%A2-kda-tdp-43-in-mouse-seminiferous-epithelium
#6
Hari Prasad Osuru, Patcharin Pramoonjago, Mayuresh Abhyankar, Eric Swanson, LaToya Ann Roker, Helen Cathro, Prabhakara P Reddi
TDP-43 (TAR DNA-binding protein of 43 kDa) is an evolutionarily conserved, ubiquitously expressed, multi-functional DNA/RNA-binding protein with roles in gene transcription, mRNA splicing, stability, transport, micro RNA biogenesis, and suppression of transposons. Aberrant expression of TDP-43 in testis and sperm was recently shown to be associated with male infertility, which highlights the need to understand better the expression of TDP-43 in the testis. We previously cloned TDP-43 from a mouse testis cDNA library, and showed that it functions as a transcriptional repressor, and regulates the precise spatiotemporal expression of the Acrv1 gene, which encodes the acrosomal protein SP-10, during spermatogenesis...
June 10, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28598082/-expression-of-micro-rna-218-in-cervical-cancer-and-its-effect-on-proliferation-apoptosis-and-invasion-of-hela-cells
#7
Yu-Rong Shi, Jian Liu, Wei He, Yin Yang
OBJECTIVES: To investigate the expression and clinical significance of microRNA-218(miR-218)in human cervical cancer and the effects ofmiR-218 on proliferation, cell apoptosis and invasion of HeLa cells. METHODS: QRT-PCR was used to detect the expression ofmiR-218 in 23 cases of normal cervical tissues and 114 cases of cervical cancer, and the relationship between the expression and the clinicopathological features was analyzed; HeLa cells were devided into three groups: non transfection (control group), transfected with empty liposomes negative control goup (NC group), transfected with miR-218 mimic (miR-218M group)...
September 2016: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/28571562/advantages-and-limitations-of-current-biomarker-research-from-experimental-research-to-clinical-application
#8
Seung-Hyun Kim, Christel Weiß, Ursula Hoffmann, Martin Borggrefe
BACKGROUND: Biomarkers are indispensable tools for screening, diagnosis, and prognosis in cardiovascular diseases and their clinical application increases steadily. As cardiovascular diseases include various pathophysiological processes, no single biomarker, even natriuretic peptides, can be regarded as ideal fulfilling all necessary criteria for a comprehensive diagnostic or prognostic assessment revealing optimal clinical application. Hence, the multi-marker approaches using different biomarkers reflecting different pathophysiologies were highlighted recently...
May 31, 2017: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/28571552/micro-rna-profiling-as-a-predictor-of-clinical-outcomes-for-head-and-neck-cancer-patients
#9
Tatyana Isayeva, Margaret Brandwein-Gensler, Maheshika Somarathna, Lakisha D Moore-Smith, Timmy Lee
Head and neck cancer is one of the leading malignancies worldwide. Due to the lack of symptoms in the early stage of the disease, about two thirds of patients present with locally advanced disease at the time of diagnosis. Even with significantly improved survival rates over the past two decades due to advanced imaging and treatment modalities, locoregional recurrence rates in patients with advanced disease ranges from 16% to 35%. Alternative therapeutic targets are being developed to improve survival outcomes...
May 31, 2017: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/28570594/automated-high-throughput-nucleic-acid-purification-from-formalin-fixed-paraffin-embedded-tissue-samples-for-next-generation-sequence-analysis
#10
Simon Haile, Pawan Pandoh, Helen McDonald, Richard D Corbett, Philip Tsao, Heather Kirk, Tina MacLeod, Martin Jones, Steve Bilobram, Denise Brooks, Duane Smailus, Christian Steidl, David W Scott, Miruna Bala, Martin Hirst, Diane Miller, Richard A Moore, Andrew J Mungall, Robin J Coope, Yussanne Ma, Yongjun Zhao, Rob A Holt, Steven J Jones, Marco A Marra
Curation and storage of formalin-fixed, paraffin-embedded (FFPE) samples are standard procedures in hospital pathology laboratories around the world. Many thousands of such samples exist and could be used for next generation sequencing analysis. Retrospective analyses of such samples are important for identifying molecular correlates of carcinogenesis, treatment history and disease outcomes. Two major hurdles in using FFPE material for sequencing are the damaged nature of the nucleic acids and the labor-intensive nature of nucleic acid purification...
2017: PloS One
https://www.readbyqxmd.com/read/28564620/survivin-in-autoimmune-diseases
#11
REVIEW
G Gravina, C Wasén, M J Garcia-Bonete, M Turkkila, M C Erlandsson, S Töyrä Silfverswärd, M Brisslert, R Pullerits, K M Andersson, G Katona, M I Bokarewa
Survivin is a protein functionally important for cell division, apoptosis, and possibly, for micro-RNA biogenesis. It is an established marker of malignant cell transformation. In non-malignant conditions, the unique properties of survivin make it indispensable for homeostasis of the immune system. Indeed, it is required for the innate and adaptive immune responses, controlling differentiation and maintenance of CD4(+) and CD8(+) memory T-cells, and in B cell maturation. Recently, survivin has emerged as an important player in the pathogenesis of autoimmune diseases...
May 28, 2017: Autoimmunity Reviews
https://www.readbyqxmd.com/read/28560603/micro-rna-130a-3p-regulates-gemcitabine%C3%A2-resistance-via-pparg-in-cholangiocarcinoma
#12
Kei Asukai, Koichi Kawamoto, Hidetoshi Eguchi, Masamitsu Konno, Ayumu Asai, Yoshifumi Iwagami, Daisaku Yamada, Tadafumi Asaoka, Takehiro Noda, Hiroshi Wada, Kunihito Gotoh, Naohiro Nishida, Taroh Satoh, Yuichiro Doki, Masaki Mori, Hideshi Ishii
BACKGROUND: The prognosis of cholangiocarcinoma (CCA) is so poor that its chemoresistance needs to be reduced. In this study, we focused on the microRNAs (miRNAs) associated with gemcitabine resistance of CCA and assessed the clinical significance of miRNAs and their target genes. METHODS: We performed miRNA microarray analysis for two CCA cell lines (CCLP-1 and MzChA-1) and their gemcitabine-resistant (GR) cells. An miR-130a-3p mimic was induced into CCA cells using lipofection, and we used pioglitazone as a peroxisome proliferator-activated receptor-γ (PPARγ) agonist in vitro...
May 30, 2017: Annals of Surgical Oncology
https://www.readbyqxmd.com/read/28558802/serum-micro-rna-profiles-in-patients-with-autosomal-dominant-polycystic-kidney-disease-according-to-hypertension-and-renal-function
#13
Ismail Kocyigit, Serpil Taheri, Elif Funda Sener, Eray Eroglu, Fahir Ozturk, Aydin Unal, Kezban Korkmaz, Gokmen Zararsiz, Murat Hayri Sipahioglu, Yusuf Ozkul, Bulent Tokgoz, Oktay Oymak, Tevfik Ecder, Jonas Axelsson
BACKGROUND: Autosomal dominant polycystic kidney disease (ADPKD) is a common hereditary disorder with unclear disease mechanism. Currently, overt hypertension and increased renal volume are the best predictors of renal function. In this study, we assessed the usefulness of selected circulating microRNAs (miRs) to predict disease progress in a cohort with ADPKD. METHODS: Eighty ADPKD patients (44.6 ± 12.7 years, 40% female, 65% hypertensive) and 50 healthy subjects (HS; 45...
May 30, 2017: BMC Nephrology
https://www.readbyqxmd.com/read/28534371/the-effect-of-mir-21-on-swoz2-glioma-cells-and-its-biological-mechanism
#14
Shen-Jie Li, Jie Zhou, Ling Zhang, Wei Xiang, Qi Hu, Yong-Yue He, Li-Gang Chen
PURPOSE: To investigate the role of micro RNA-21 (miR- 21) in human glioma cells and its potential disease-causing mechanism. METHODS: jetPRIME was used to transfect the miR-21- mimics and its negative control into SWOZ2 human glioma cells. Real-time fluorescence quantitative PCR assay was used to measure differences in the expression of miR-21 in SWOZ2 glioma cells, SWOZ2-miR-21-mimics cells, and control cells. Cell counting kit-8 assay was used to measure the activity of SWOZ2 glioma cells and SWOZ2-miR-21- mimics cells, and Western blot was used to measure PTEN, p-Akt, and P-glycoprotein (P-gp)...
March 2017: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
https://www.readbyqxmd.com/read/28532427/microrna-275-and-its-target-vitellogenin-2-are-crucial-in-ovary-development-and-blood-digestion-of-haemaphysalis-longicornis
#15
Jiawei Hao, Jin Luo, Ze Chen, Qiaoyun Ren, Jinxia Guo, Xiaocui Liu, Qiuyu Chen, Feng Wu, Zhen Wang, Jianxun Luo, Hong Yin, Hui Wang, Guangyuan Liu
BACKGROUND: The hard tick Haemaphysalis longicornis is widely distributed in eastern Asia, New Zealand and Australia and is considered the major vector of Theileria and Babesia, harmful parasites to humans and animals. Female ticks need successful blood meals to complete the life-cycle. Therefore, elucidation of the underlying molecular mechanisms of H. longicornis development and reproduction is considered important for developing control strategies against the tick and tick-borne pathogens...
May 22, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28529818/high-protein-fish-oil-diet-prevents-hepatic-steatosis-in-noncnzo10-mice-association-with-diet-genetics-regulated-micro-rnas
#16
Nikhil Adi, Jennipher Adi, Roberta Marques Lassance-Soares, Paul Kurlansky, Hong Yu, Keith A Webster
OBJECTIVE: NONcNZO10 (NZ10) mice are predisposed to obesity and develop type 2 diabetes (T2D) and hepatic steatosis even when maintained on a control diet (CD) of 6% fat. Studies were designed to determine whether this extreme susceptibility phenotype could be alleviated by diet and if so the molecular targets of diet. METHODS: NZ10 and SWR/J (SWR) control mice were fed a CD or a test diet of high protein and fish oil (HPO) for 19 weeks and then analyzed for steatosis, blood chemistry, hepatic gene and micro-RNA expression...
June 2016: Journal of Diabetes & Metabolism
https://www.readbyqxmd.com/read/28526928/exosomes-in-cardiovascular-medicine
#17
REVIEW
Iain M Dykes
Exosomes are small, extracellular membrane-bound particles that mediate intercellular transport of a cytosolic cargo. Exosomal transfer of micro-RNA can modify gene expression in targeted cells. Exosome-based endocrine/paracrine signaling has been shown to be involved in a wide range of physiological processes including those associated with cardiovascular injury and disease, but remains relatively poorly understood. Exosomes offer great potential to the clinical field, with applications in both diagnostics and therapeutics...
May 19, 2017: Cardiology and Therapy
https://www.readbyqxmd.com/read/28524153/transcriptome-analysis-of-mirna-lncrna-mrna-interactions-in-the-malignant-transformation-process-of-gastric-cancer-initiation
#18
Y Mao, R Liu, H Zhou, S Yin, Q Zhao, X Ding, H Wang
Gastric cancer is a common heterogeneous malignancy that is pathologically characterized by the development of chronic atrophic gastritis and dysplasia of the epithelium. The pathogenic mechanisms at the molecular level are completely unclear. In the present study, we first address the expression network and miRNA-lncRNA-mRNA interaction in the malignant transformation process from normal mucosa and atrophic gastritis to a tumor. First, the long non-coding RNA (lncRNA), micro RNA (miRNA) and messenger RNA (mRNA) expression profiles of normal gastric mucosa, atrophic gastritis, gastric cancer and the peri-tumor tissues were analyzed using microarrays...
June 2017: Cancer Gene Therapy
https://www.readbyqxmd.com/read/28515288/motor-recovery-beginning-23-years-after-ischemic-stroke
#19
Peter Sörös, Robert Teasell, Daniel F Hanley, J David Spence
It is widely believed that most stroke recovery occurs within 6 months, with little benefit of physiotherapy or other modalities beyond a year. We report a remarkable case of stroke recovery beginning 23 years after a severe stroke due to embolization from the innominate artery and subclavian artery, resulting from compression of the right subclavian artery by a cervical rib. The patient had a large right fronto-parietal infarction with severe left hemiparesis, and a totally non-functional spastic left hand...
May 17, 2017: Journal of Neurophysiology
https://www.readbyqxmd.com/read/28512462/de-novo-transcriptome-profiling-of-flowers-flower-pedicels-and-pods-of-lupinus-luteus-yellow-lupine-reveals-complex-expression-changes-during-organ-abscission
#20
Paulina Glazinska, Waldemar Wojciechowski, Milena Kulasek, Wojciech Glinkowski, Katarzyna Marciniak, Natalia Klajn, Jacek Kesy, Jan Kopcewicz
Yellow lupine (Lupinus luteus L., Taper c.), a member of the legume family (Fabaceae L.), has an enormous practical importance. Its excessive flower and pod abscission represents an economic drawback, as proper flower and seed formation and development is crucial for the plant's productivity. Generative organ detachment takes place at the basis of the pedicels, within a specialized group of cells collectively known as the abscission zone (AZ). During plant growth these cells become competent to respond to specific signals that trigger separation and lead to the abolition of cell wall adhesion...
2017: Frontiers in Plant Science
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