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https://www.readbyqxmd.com/read/28102740/update-on-functional-and-genetic-laboratory-assays-for-the-detection-of-platelet-microvesicles
#1
Maxime Laroche, Claire Dunois, Anne Marie Vissac, Jean Amiral
Functional and genetic assays for measuring platelet microvesicles (PMVs) are presented and discussed. Functional assays concern two groups of methods: a) homogeneous assays using the cofactor activity of phospholipids (PPLs) contained in PMVs and present in assayed plasmas, and a coagulation or a thrombin generation assay (TGA) as "end points"; b) capture-based assays, in which PMVs bind to an immobilized ligand, such as Annexin V in the presence of calcium, or monoclonal antibodies (MoAbs) specific for membrane proteins...
January 19, 2017: Platelets
https://www.readbyqxmd.com/read/28100188/high-expression-of-micro-rna-135a-in-hepatocellular-carcinoma-is-associated-with-recurrence-within-12%C3%A2-months-after-resection
#2
Johann von Felden, Denise Heim, Kornelius Schulze, Till Krech, Florian Ewald, Björn Nashan, Ansgar W Lohse, Henning Wege
BACKGROUND: Hepatocellular carcinoma has a dismal prognosis due to recurrence rates of up to 70% after curative resection. Early recurrence is driven by synchronous microscopic intrahepatic metastases. The predictive value of histological parameters is discussed controversially and adjuvant therapy is not established. The aim of this study was to identify patients at high risk for early intrahepatic recurrence by expression profiling of selected micro RNAs. METHODS: In 52 patients undergoing HCC resection between 2011 and 2014, liver and tumor tissue was collected during surgery...
January 18, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28088520/palmitic-acid-increases-invasiveness-of-pancreatic-cancer-cells-aspc-1-through-tlr4-ros-nf-%C3%AE%C2%BAb-mmp-9-signaling-pathway
#3
Makena J Binker-Cosen, Daniel Richards, Brenda Oliver, Herbert Y Gaisano, Marcelo G Binker, Laura I Cosen-Binker
Pancreatic cancer (PC) is an aggressive malady with proclivity for early metastasis. Overexpression of toll-like receptor 4 (TLR4) in pancreatic ductal adenocarcinoma, the most common type of pancreatic malignancy, correlates to tumor size, lymph node involvement, venous invasion and pathological stage. Lipopolysaccharides (LPS) are natural TLR4 ligands that have been shown to increase the invasive ability of PC cells. However, rapid inactivation of circulating LPS and low systemic absorption of inhaled LPS from the bronchoalveolar compartment make other agonists such as saturated fatty acids more suitable to be considered for TLR4-related cell invasiveness...
January 11, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28078570/micro-rna-expression-in-muscle-and-fiber-morphometry-in-myotonic-dystrophy-type-1
#4
Chiara Fritegotto, Chiara Ferrati, Valentina Pegoraro, Corrado Angelini
We aimed to explore the cellular action of micro-RNAs that are non-coding-RNAs modulating gene expression, whose expression is dysregulated in myotonic dystrophy (DM1). Basic procedure was to measure the levels of muscle-specific myo-miRNAs (miR-1, miR-133a/b, miR-206) in muscle of 12 DM1 patients. Muscle fiber morphometry and a new grading of histopathological severity score were used to compare specific myo-miRNA level and fiber atrophy. We found that the levels of miR-1 and miR-133a/b were significantly decreased, while miR-206 was significantly increased as compared to controls...
January 11, 2017: Neurological Sciences
https://www.readbyqxmd.com/read/28077169/distinct-5-methylcytosine-profiles-in-poly-a-rna-from-mouse-embryonic-stem-cells-and-brain
#5
Thomas Amort, Dietmar Rieder, Alexandra Wille, Daria Khokhlova-Cubberley, Christian Riml, Lukas Trixl, Xi-Yu Jia, Ronald Micura, Alexandra Lusser
BACKGROUND: Recent work has identified and mapped a range of posttranscriptional modifications in mRNA, including methylation of the N6 and N1 positions in adenine, pseudouridylation, and methylation of carbon 5 in cytosine (m5C). However, knowledge about the prevalence and transcriptome-wide distribution of m5C is still extremely limited; thus, studies in different cell types, tissues, and organisms are needed to gain insight into possible functions of this modification and implications for other regulatory processes...
January 5, 2017: Genome Biology
https://www.readbyqxmd.com/read/28074664/recent-advances-in-molecular-biomarkers-for-diabetes-mellitus-a-systematic-review
#6
Ty Lees, Najah Nassif, Ann Simpson, Fatima Shad-Kaneez, Rose Martiniello-Wilks, Yiguang Lin, Allan Jones, Xianqin Qu, Sara Lal
CONTEXT: Diabetes is a growing global metabolic epidemic. Current research is focusing on exploring how the biological processes and clinical outcomes of diabetes are related and developing novel biomarkers to measure these relationships, as this can subsequently improve diagnostic, therapeutic and management capacity. OBJECTIVE: To identify the most recent advances in molecular biomarkers of diabetes and directions that warrant further research. METHODS: Using a systematic search strategy, the MEDLINE, CINAHL and OVID MEDLINE databases were canvassed for articles that investigated molecular biomarkers for diabetes...
January 11, 2017: Biomarkers: Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
https://www.readbyqxmd.com/read/28070818/entangling-relation-of-micro-rna-let7-mirna-200-and-mirna-125-with-various-cancers
#7
REVIEW
Nosheen Masood, Azra Yasmin
Involvement of micro RNAs (miRNA) is currently the focus for cancer studies as they effect the post transcriptional expression of different genes. Let-7 family is among the firstly discovered miRNAs that play important role in cell proliferation and dysregulation leading to cell based diseases including cancer. Another family, miRNA-200 prevents transformation of cell to malignant form and tumor formation by interacting with epidermal mesenchymal transition (EMT). Similarly miRNA-125 controls apoptosis and proliferation by affecting multiple genes involved in transcription, immunological defense, resistance against viral and bacterial infections that ultimately leads to cell proliferation, metastasis and finally cancer...
January 9, 2017: Pathology Oncology Research: POR
https://www.readbyqxmd.com/read/28062497/increased-proangiogenic-activity-of-mobilized-cd34-progenitor-cells-of-patients-with-acute-st-segment-elevation-myocardial-infarction-role-of-differential-micro-rna-378-expression
#8
Christian Templin, Julia Volkmann, Maximilian Y Emmert, Pavani Mocharla, Maja Müller, Nicolle Kraenkel, Jelena R Ghadri, Martin Meyer, Beata Styp-Rekowska, Sylvie Briand, Roland Klingenberg, Milosz Jaguszewski, Christian M Matter, Valentin Djonov, Francois Mach, Stephan Windecker, Simon P Hoerstrup, Thomas Thum, Thomas F Lüscher, Ulf Landmesser
OBJECTIVE: Proangiogenic effects of mobilized bone marrow-derived stem/progenitor cells are essential for cardiac repair after myocardial infarction. Micro-RNAs (miRNA/miR) are key regulators of angiogenesis. We investigated the differential regulation of angio-miRs, that is, miRNAs regulating neovascularization, in mobilized CD34(+) progenitor cells obtained from patients with an acute ST-segment-elevation myocardial infarction (STEMI) as compared with those with stable coronary artery disease or healthy subjects...
December 29, 2016: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/28057790/novel-risk-markers-and-risk-assessments-for-cardiovascular-disease
#9
REVIEW
Mark R Thomas, Gregory Y H Lip
The use of risk markers has transformed cardiovascular medicine, exemplified by the routine assessment of troponin, for both diagnosis and assessment of prognosis in patients with chest pain. Clinical risk factors form the basis for risk assessment of cardiovascular disease and the addition of biochemical, cellular, and imaging parameters offers further refinement. Identifying novel risk factors may allow greater risk stratification and a steady, but gradual progression toward precision medicine. Indeed, the generation of data in this area of research is explosive and when combined with new technologies and techniques provides the potential for more refined, targeted approaches to cardiovascular medicine...
January 6, 2017: Circulation Research
https://www.readbyqxmd.com/read/28051259/microrna-145-inhibits-tumour-growth-and-metastasis-in-osteosarcoma-by-targeting-cyclin-dependent-kinase-cdk6
#10
Y Li, J Liu, Z-Z Liu, W-B Wei
OBJECTIVE: Osteosarcoma (OS) is reported to be one of the most familiar forms of the primary malignant tumor to adolescents and adults, which is usually found in their long bones. Evidence and data have shown that abnormality in micro-RNA expression is closely related to tumorigenesis. The aim of this study is to investigate the expression, function and underlying mechanism of miR-145 in OS. MATERIALS AND METHODS: MiR-145 expression in tumor tissues of patients and cell lines were detected by SYBR green qPCR...
December 2016: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28050800/micro-rna-204-participates-in-tmprss2-erg-regulation-and-androgen-receptor-reprogramming-in-prostate-cancer
#11
Krassimira Todorova, Metodi V Metodiev, Gergana Metodieva, Milcho Mincheff, Nelson Fernández, Soren Hayrabedyan
Cancer progression is driven by genome instability incurred rearrangements such as transmembrane protease, serine 2 (TMPRSS2)/v-ets erythroblastosis virus E26 oncogene (ERG) that could possibly turn some of the tumor suppressor micro-RNAs into pro-oncogenic ones. Previously, we found dualistic miR-204 effects, acting either as a tumor suppressor or as an oncomiR in ERG fusion-dependent manner. Here, we provided further evidence for an important role of miR-204 for TMPRSS2/ERG and androgen receptor (AR) signaling modulation and fine tuning that prevents TMPRSS2/ERG overexpression in prostate cancer...
January 3, 2017: Hormones & Cancer
https://www.readbyqxmd.com/read/28038755/nano-polyelectrolyte-complexes-of-carboxymethyl-dextran-and-chitosan-to-improve-chitosan-mediated-delivery-of-mir-145
#12
Farnaz Sadat Mirzazadeh Tekie, Melika Kiani, Amir Zakerian, Faezeh Pilevarian, Akram Assali, Masoud Soleimani, Rassoul Dinarvand, Ehsan Arefian, Amir Atashi, Mohsen Amini, Fatemeh Atyabi
Chitosan (Ch) nanoparticles have emerged as a promising vector for gene delivery, nonetheless slow dissociation rate of the nanoparticles in cytoplasm is a drawback of using Ch. Herein, the Ch-mediated gene delivery was improved using PECs of Ch and carboxymethyl dextran (CMD) to transfer the micro RNA-145 (miR-145). The optimized nano PEC preparation method and effects of Ch molecular weight (Mw) and a CMD to Ch molar ratio (CMD:Ch) on physical characteristics and in vitro efficacy of the nano PECs was determined...
March 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28035318/circulating-biomarkers-in-bladder-cancer
#13
REVIEW
Lakshminarayanan Nandagopal, Guru Sonpavde
Bladder cancer is a molecularly heterogeneous disease characterized by multiple unmet needs in the realm of diagnosis, clinical staging, monitoring and therapy. There is an urgent need to develop precision medicine for advanced urothelial carcinoma. Given the difficulty of serial analyses of metastatic tumor tissue to identify resistance and new therapeutic targets, development of non-invasive monitoring using circulating molecular biomarkers is critically important. Although the development of circulating biomarkers for the management of bladder cancer is in its infancy and may currently suffer from lower sensitivity of detection, they have inherent advantages owing to non-invasiveness...
October 27, 2016: Bladder Cancer
https://www.readbyqxmd.com/read/28000202/micro-rna-signature-of-lymphovascular-space-involvement-in-type-1-endometrial-cancer
#14
Geoffroy Canlorbe, Mathieu Castela, Sofiane Bendifallah, Zhe Wang, Marine Lefevre, Nathalie Chabbert-Buffet, Selim Aractingi, Emile DaraÏ, Céline Méhats, Marcos Ballester
OBJECTIVE: Lymphovascular space involvement (LVSI) is a major prognostic factor in type 1 endometrial cancer (EC). However, its use has been criticized because of poor subjectivity. MicroRNA signatures have recently been linked to EC pathologic characteristics. The aim of this study was to evaluate whether microRNA profiles of type 1 EC can be related to LVSI status and used as a tool to adapt therapy. STUDY DESIGN: MicroRNA expression was assessed by chip analysis and qRT-PCR in 12 formalin-fixed paraffin-embedded grade 2 EC specimens with positive LVSI and in 12 specimens with negative LVSI...
December 21, 2016: Histology and Histopathology
https://www.readbyqxmd.com/read/28000097/an-overview-of-epigenetics-in-chinese-medicine-researches
#15
REVIEW
Xue-Qing Hu, Shi-Bing Su
Chinese medicine (CM) has contributed to human health care for several thousand years and is still popular in Asian countries. But the mechanisms underlying CM remain obscure, and need to be clarified by modern biological subjects and methods. Recently, CM researches on epigenetics have gained obvious improvement following rapidly developed molecular technologies. In this review, we revealed mutual characteristics of epigenetics and CM, such as holism, yin-yang dynamic nature, reversibility and balance, and visceral manifestation...
December 20, 2016: Chinese Journal of Integrative Medicine
https://www.readbyqxmd.com/read/27992614/a-single-nucleotide-polymorphism-in-the-rasgrf2-gene-is-associated-with-alcoholic-liver-cirrhosis-in-men
#16
Ignacio Novo-Veleiro, Clara Cieza-Borrella, Isabel Pastor, Antonio-Javier Chamorro, Francisco-Javier Laso, Rogelio González-Sarmiento, Miguel Marcos
BACKGROUND: Genetic polymorphisms in the RAS gene family are associated with different diseases, which may include alcohol-related disorders. Previous studies showed an association of the allelic variant rs26907 in RASGRF2 gene with higher alcohol intake. Additionally, the rs61764370 polymorphism in the KRAS gene is located in a binding site for the let-7 micro-RNA family, which is potentially involved in alcohol-induced inflammation. Therefore, this study was designed to explore the association between these two polymorphisms and susceptibility to alcoholism or alcoholic liver disease (ALD)...
2016: PloS One
https://www.readbyqxmd.com/read/27941332/overexpression-of-tgf-%C3%AE-inducible-microrna-143-in-zebrafish-leads-to-impairment-of-the-glomerular-filtration-barrier-by-targeting-proteoglycans
#17
Janina Müller-Deile, Finn Gellrich, Heiko Schenk, Patricia Schroder, Jenny Nyström, Johan Lorenzen, Hermann Haller, Mario Schiffer
BACKGROUND: TGF-β is known as an important stress factor of podocytes in glomerular diseases. Apart from activation of direct pro-apoptotic pathways we wanted to analyze micro-RNA (miRs) driven regulation of components involved in the integrity of the glomerular filtration barrier induced by TGF-β. Since miR-143-3p (miR-143) is described as a TGF-β inducible miR in other cell types, we examined this specific miR and its ability to induce glomerular pathology. METHODS: We analyzed miR-143 expression in cultured human podocytes after stimulation with TGF-β...
2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27935135/micro-rna-550a-interferes-with-vitamin-d-metabolism-in-peripheral-b-cells-of-patients-with-diabetes
#18
Jinggui He, Xiyun Guo, Zhi-Qiang Liu, Ping-Chang Yang, Shaobo Yang
The pathogenesis of diabetes is to be further investigated. Vitamin D3 (VitD3) can improve diabetes. Micro RNAs (miR) are involved in regulating cell activities. This study tests a hypothesis that miR-550a interferes with the metabolism of VitD3 in peripheral B cells. In this study, blood samples were collected from patients with diabetes and healthy persons. The B cells were isolated from the blood samples to be treated with tumor necrosis factor (TNF)-α. The B cells were then collected and analyzed for the expression of miR-550a and cyp27b1...
December 2016: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/27903834/saliva-diagnostics-current-views-and-directions
#19
Karolina Elżbieta Kaczor-Urbanowicz, Carmen Martin Carreras-Presas, Katri Aro, Michael Tu, Franklin Garcia-Godoy, David Tw Wong
In this review, we provide an update on the current and future applications of saliva for diagnostic purposes. There are many advantages of using saliva as a biofluid. Its collection is fast, easy, inexpensive, and non-invasive. In addition, saliva, as a "mirror of the body," can reflect the physiological and pathological state of the body. Therefore, it serves as a diagnostic and monitoring tool in many fields of science such as medicine, dentistry, and pharmacotherapy. Introduced in 2008, the term "Salivaomics" aimed to highlight the rapid development of knowledge about various "omics" constituents of saliva, including: proteome, transcriptome, micro-RNA, metabolome, and microbiome...
November 29, 2016: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/27893988/endothelin-signaling-in-bone
#20
REVIEW
Jasmin Kristianto, Michael G Johnson, Rafia Afzal, Robert D Blank
The endothelin (ET) system includes 3 small peptide hormones and a pair of G protein-coupled receptors. All 3 ETs are secreted as biologically inert precursors that must be activated by proteolytic cleavage after secretion. This reaction can be catalyzed by a pair of specific, membrane-bound extracellular ET converting enzymes or by nonspecific tissue proteases. The ET1/EDNRA axis is essential in development, with knockout mice for either displaying a similar phenotype featuring multiple defects of the craniofacial skeleton and cardiac outflow tract...
November 11, 2016: Translational Research: the Journal of Laboratory and Clinical Medicine
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