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Micro-rna

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https://www.readbyqxmd.com/read/28534371/the-effect-of-mir-21-on-swoz2-glioma-cells-and-its-biological-mechanism
#1
Shen-Jie Li, Jie Zhou, Ling Zhang, Wei Xiang, Qi Hu, Yong-Yue He, Li-Gang Chen
PURPOSE: To investigate the role of micro RNA-21 (miR- 21) in human glioma cells and its potential disease-causing mechanism. METHODS: jetPRIME was used to transfect the miR-21- mimics and its negative control into SWOZ2 human glioma cells. Real-time fluorescence quantitative PCR assay was used to measure differences in the expression of miR-21 in SWOZ2 glioma cells, SWOZ2-miR-21-mimics cells, and control cells. Cell counting kit-8 assay was used to measure the activity of SWOZ2 glioma cells and SWOZ2-miR-21- mimics cells, and Western blot was used to measure PTEN, p-Akt, and P-glycoprotein (P-gp)...
March 2017: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
https://www.readbyqxmd.com/read/28532427/microrna-275-and-its-target-vitellogenin-2-are-crucial-in-ovary-development-and-blood-digestion-of-haemaphysalis-longicornis
#2
Jiawei Hao, Jin Luo, Ze Chen, Qiaoyun Ren, Jinxia Guo, Xiaocui Liu, Qiuyu Chen, Feng Wu, Zhen Wang, Jianxun Luo, Hong Yin, Hui Wang, Guangyuan Liu
BACKGROUND: The hard tick Haemaphysalis longicornis is widely distributed in eastern Asia, New Zealand and Australia and is considered the major vector of Theileria and Babesia, harmful parasites to humans and animals. Female ticks need successful blood meals to complete the life-cycle. Therefore, elucidation of the underlying molecular mechanisms of H. longicornis development and reproduction is considered important for developing control strategies against the tick and tick-borne pathogens...
May 22, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28529818/high-protein-fish-oil-diet-prevents-hepatic-steatosis-in-noncnzo10-mice-association-with-diet-genetics-regulated-micro-rnas
#3
Nikhil Adi, Jennipher Adi, Roberta Marques Lassance-Soares, Paul Kurlansky, Hong Yu, Keith A Webster
OBJECTIVE: NONcNZO10 (NZ10) mice are predisposed to obesity and develop type 2 diabetes (T2D) and hepatic steatosis even when maintained on a control diet (CD) of 6% fat. Studies were designed to determine whether this extreme susceptibility phenotype could be alleviated by diet and if so the molecular targets of diet. METHODS: NZ10 and SWR/J (SWR) control mice were fed a CD or a test diet of high protein and fish oil (HPO) for 19 weeks and then analyzed for steatosis, blood chemistry, hepatic gene and micro-RNA expression...
June 2016: Journal of Diabetes & Metabolism
https://www.readbyqxmd.com/read/28526928/exosomes-in-cardiovascular-medicine
#4
REVIEW
Iain M Dykes
Exosomes are small, extracellular membrane-bound particles that mediate intercellular transport of a cytosolic cargo. Exosomal transfer of micro-RNA can modify gene expression in targeted cells. Exosome-based endocrine/paracrine signaling has been shown to be involved in a wide range of physiological processes including those associated with cardiovascular injury and disease, but remains relatively poorly understood. Exosomes offer great potential to the clinical field, with applications in both diagnostics and therapeutics...
May 19, 2017: Cardiology and Therapy
https://www.readbyqxmd.com/read/28524153/transcriptome-analysis-of-mirna-lncrna-mrna-interactions-in-the-malignant-transformation-process-of-gastric-cancer-initiation
#5
Y Mao, R Liu, H Zhou, S Yin, Q Zhao, X Ding, H Wang
Gastric cancer is a common heterogeneous malignancy that is pathologically characterized by the development of chronic atrophic gastritis and dysplasia of the epithelium. The pathogenic mechanisms at the molecular level are completely unclear. In the present study, we first address the expression network and miRNA-lncRNA-mRNA interaction in the malignant transformation process from normal mucosa and atrophic gastritis to a tumor. First, the long non-coding RNA (lncRNA), micro RNA (miRNA) and messenger RNA (mRNA) expression profiles of normal gastric mucosa, atrophic gastritis, gastric cancer and the peri-tumor tissues were analyzed using microarrays...
May 19, 2017: Cancer Gene Therapy
https://www.readbyqxmd.com/read/28515288/motor-recovery-beginning-23-years-after-ischemic-stroke
#6
Peter Sörös, Robert Teasell, Daniel F Hanley, J David Spence
It is widely believed that most stroke recovery occurs within 6 months, with little benefit of physiotherapy or other modalities beyond a year. We report a remarkable case of stroke recovery beginning 23 years after a severe stroke due to embolization from the innominate artery and subclavian artery, resulting from compression of the right subclavian artery by a cervical rib. The patient had a large right fronto-parietal infarction with severe left hemiparesis, and a totally non-functional spastic left hand...
May 17, 2017: Journal of Neurophysiology
https://www.readbyqxmd.com/read/28512462/de-novo-transcriptome-profiling-of-flowers-flower-pedicels-and-pods-of-lupinus-luteus-yellow-lupine-reveals-complex-expression-changes-during-organ-abscission
#7
Paulina Glazinska, Waldemar Wojciechowski, Milena Kulasek, Wojciech Glinkowski, Katarzyna Marciniak, Natalia Klajn, Jacek Kesy, Jan Kopcewicz
Yellow lupine (Lupinus luteus L., Taper c.), a member of the legume family (Fabaceae L.), has an enormous practical importance. Its excessive flower and pod abscission represents an economic drawback, as proper flower and seed formation and development is crucial for the plant's productivity. Generative organ detachment takes place at the basis of the pedicels, within a specialized group of cells collectively known as the abscission zone (AZ). During plant growth these cells become competent to respond to specific signals that trigger separation and lead to the abolition of cell wall adhesion...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28491088/novel-biomarkers-for-cardiovascular-risk-prediction
#8
REVIEW
Juan Wang, Guo-Juan Tan, Li-Na Han, Yong-Yi Bai, Miao He, Hong-Bin Liu
Cardiovascular disease (CVD) is the leading cause of death and disability worldwide. The primary prevention of CVD is dependent upon the ability to identify high-risk individuals long before the development of overt events. This highlights the need for accurate risk stratification. An increasing number of novel biomarkers have been identified to predict cardiovascular events. Biomarkers play a critical role in the definition, prognostication, and decision-making regarding the management of cardiovascular events...
February 2017: Journal of Geriatric Cardiology: JGC
https://www.readbyqxmd.com/read/28486552/identification-of-novel-mirnas-and-mirna-expression-profiling-in-embryogenic-tissues-of-picea-balfouriana-treated-by-6-benzylaminopurine
#9
Qingfen Li, Cheng Deng, Yan Xia, Lisheng Kong, Hanguo Zhang, Shougong Zhang, Junhui Wang
Here, we compared miRNA expression profiles in embryonic cell cultures of the conifer Picea balfouriana following application of the synthetic cytokinin 6-benzylaminopurine (6-BAP). We used next-generation sequencing to analyze three libraries of small RNAs from the treated embryogenic cell cultures and generated 24,000,000 raw reads from each of the libraries. Over 70 differentially regulated micro RNA (miRNA) families (≥2 fold change in expression) were identified between pairs of treatments. A quantitative analysis showed that miR3633 and miR1026 were upregulated in tissues with the highest embryogenic ability...
2017: PloS One
https://www.readbyqxmd.com/read/28482074/carbon-ion-irradiation-abrogates-lin28b-induced-x-ray-resistance-in-melanoma-cells
#10
Seong-Joon Park, Kyu Heo, Chulwon Choi, Kwangmo Yang, Akiko Adachi, Hiroko Okada, Yukari Yoshida, Tatsuya Ohno, Takashi Nakano, Akihisa Takahashi
The Lin28/let-7 axis plays an important role in tumor initiation and developmental processes. Lin28B is upregulated in a variety of cancers, and its overexpression enhances cancer cell proliferation and radioresistance through the suppression of let-7 micro RNA expression. In this study, we investigated the role of the Lin28/let7 axis as a target for radiosensitization of melanoma cancer cells. The overexpression of Lin28B reduced mature let-7 microRNA expression in melanoma cell lines, and enhanced the sphere-forming ability of melanoma cell lines, which is a characteristic of cancer stem cell (CSC) populations...
May 8, 2017: Journal of Radiation Research
https://www.readbyqxmd.com/read/28477403/micro-rna-21-regulates-cancer-associated-fibroblast-mediated-frug-resistance-in-pancreatic-cancer
#11
Lulin Zhang, Jun Yao, Wenyao Li, Ce Zhang
Pancreatic adenocarcinoma (PDAC) is one of the leading cause of cancer death due to its highly aggressive biological nature and resistance to chemotherapies. Former study also indicated that miR-21 in cancer-associated fibroblasts (CAFs) is an important regulator of their activation. However, whether miR-21 in CAFs would regulate PDAC's tumor microenvironment and leading to drug resistance remain unknown. In this study, we evaluated the relationship among CAFs activation, miR-21 expression level and drug resistance using PDAC patient tumor samples...
May 5, 2017: Oncology Research
https://www.readbyqxmd.com/read/28469300/bladder-cancer-micro-rnas-as-biomolecules-for-prognostication-and-surveillance
#12
REVIEW
Nilay Mitash, Swasti Tiwari, Shalini Agnihotri, Anil Mandhani
INTRODUCTION: Bladder cancer (BC) has varied clinical behavior in terms of recurrence and progression. Current pathological characteristics are insufficient to prognosticate the outcome of a given treatment. Cellular metabolic regulatory molecules, such as micro RNA (miRNA), could be a potential biomarker to prognosticate the treatment outcomes. MATERIALS AND METHODS: PubMed and Google Scholar databases were searched for publications from 1990 to 2016, related to miRNA biogenesis, its function, and role in the pathogenesis of bladder as well as other cancers...
April 2017: Indian Journal of Urology: IJU: Journal of the Urological Society of India
https://www.readbyqxmd.com/read/28467117/evaluation-of-compound-optical-interference-in-high-content-screening
#13
Glorymar Ibáñez, Paul A Calder, Constantin Radu, Bhavneet Bhinder, David Shum, Christophe Antczak, Hakim Djaballah
Compound optical interference remains an inherent problem in chemical screening and has been well documented for biochemical assays and less so for automated microscopy-based assays. It has also been the assumption that the latter should not suffer from such interference because of the washing steps involved in the process, thus eliminating the residual nonspecific compound effects. Instead, these compounds may have no relevance to the actual target, and as such, compound optical interference contributes to a number of false-positives, resulting in a high attrition rate during subsequent follow-up studies...
April 1, 2017: SLAS Discovery
https://www.readbyqxmd.com/read/28456897/erratum-to-entangling-relation-of-micro-rna-let7-mirna-200-and-mirna-125-with-various-cancers
#14
Nosheen Masood, Zarrin Basharat, Tabeer Khan, Azra Yasmin
No abstract text is available yet for this article.
April 29, 2017: Pathology Oncology Research: POR
https://www.readbyqxmd.com/read/28453961/diabetic-nephropathy-new-insights-into-established-therapeutic-paradigms-and-novel-molecular-targets
#15
REVIEW
Dilip Sharma, Pallab Bhattacharya, Kiran Kalia, Vinod Tiwari
Diabetic nephropathy is one of the most prevalent microvascular complication in patients suffering from diabetes and is reported to be the major cause of renal failure when compared to any other kidney disease. Currently, available therapies provide only symptomatic relief and unable to treat the underlying pathophysiology of diabetic nephropathy. This review will explore new insights into the established therapeutic paradigms targeting oxidative stress, inflammation and endoplasmic reticulum stress with the focus on recent clinical developments...
April 13, 2017: Diabetes Research and Clinical Practice
https://www.readbyqxmd.com/read/28445253/micro-rna-screening-and-prediction-model-construction-for-diagnosis-of-salt-sensitive-essential-hypertension
#16
Han Qi, Zheng Liu, Bin Liu, Han Cao, Weiping Sun, Yuxiang Yan, Ling Zhang
Commonly used tests for diagnosis of salt-sensitive hypertension (SSH) are complex and time-consuming, so new methods are required. Many studies have demonstrated roles for miRNAs in hypertension; however, the diagnostic value of miRNAs has yet to be determined for human SSH. In this study, we examined miRNA expression profiles by initial high-throughput miRNA sequencing of samples from patients with salt-sensitive and salt-resistant hypertension (SSH and SRH, respectively; n = 6, both groups), followed by validation by quantitative real-time polymerase chain reaction (qRT-PCR) in a larger cohort (n = 91)...
April 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28444618/rna-dna-co-analysis-on-aged-bloodstains-from-adhesive-tapes-used-for-gunshot-residue-collection-from-hands
#17
Melanie Grabmüller, Christian Schyma, Burkhard Madea, Tim Eichhorst, Cornelius Courts
In cases of firearm related fatalities a systematic investigation at the scene of death is indispensable to differentiate between self-inflicted and homicidal gunshot injuries. A common method to preserve gunshot residues (GSR) is their collection using adhesive tapes. However, the biological material gathered at the same time by the tapes would be of special interest if backspatter, ejected from the entrance wound against the direction of fire, could be detected. In the present study we examined the success rate of co-analysis of RNA and DNA recovered from biological traces sampled with adhesive tapes...
April 25, 2017: Forensic Science, Medicine, and Pathology
https://www.readbyqxmd.com/read/28440611/the-meningioma-1-mn1-gene-is-an-independent-poor-prognostic-factor-in-adult-egyptian-acute-myeloid-leukemia-patients
#18
Roxan E Shafik, Naglaa M Hassan, Yomna M El Meligui, Hanan E Shafik
Aim: To determine the prognostic importance of meningioma 1 (MN1) gene expression levels in the context of other predictive markers for acute myeloid leukemia (AML) cases. Methods: MN1 expression was measured in 85 newly diagnosed adults younger than 60 years by real-time reverse-transcriptase polymerase chain reaction. Results: At diagnosis 67.4% of cases had elevated MN1 expression, this being associated with a worse prognosis, higher incidence of lymphadenopathy and CD34 transcript expression (p=0.02 and <0...
March 1, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28439113/microrna-expression-profiling-defines-the-impact-of-electronic-cigarettes-on-human-airway-epithelial-cells
#19
Siva Kumar Solleti, Soumyaroop Bhattacharya, Ausaf Ahmad, Qian Wang, Jared Mereness, Tirumalai Rangasamy, Thomas J Mariani
While all forms of tobacco exposure have negative health effects, the significance of exposure to electronic cigarettes (eCig) is not fully understood. Here, we studied the global effects of eCig on the micro RNA (miRNA) transcriptome in human lung epithelial cells. Primary human bronchial epithelial (NHBE) cells differentiated at air-liquid interface were exposed to eCig liquid. Exposure of NHBE to any eCig liquid resulted in the induction of oxidative stress-response genes including GCLM, GCLC, GPX2, NQO1 and HO-1...
April 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28436142/cortisol-is-associated-with-low-frequency-of-interleukin-10-producing-b-cells-in-patients-with-atherosclerosis
#20
Yizhong Huo, Yan Chu, Lixin Guo, Linli Liu, Xiaojun Xia, Tierui Wang
It is accepted that inflammation plays a critical role in the development of atherosclerosis; the pathogenesis is not clear. B-cell-produced interleukin (IL) 10 is an immune regulatory cytokine that can inhibit immune inflammation. This study tests a hypothesis that a psychological stress hormone, cortisol, suppresses IL-10 expression in peripheral B cells of patients with atherosclerosis. Peripheral blood samples were collected from patients with coronary artery atherosclerosis. B cells were isolated from the blood samples to be analyzed for the expression of IL-10 and micro RNA (miR) 98 by real-time polymerase chain reaction...
April 2017: Cell Biochemistry and Function
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