keyword
https://read.qxmd.com/read/38408562/genomic-insights-into-an-extensively-drug-resistant-and-hypervirulent-burkholderia-dolosa-n149-isolate-of-a-novel-sequence-type-st2237-from-a-vietnamese-patient-hospitalized-for-stroke
#21
JOURNAL ARTICLE
Nguyen Quang Huy, Nguyen Cam Linh, Nguyen Thai Son, Do Bich Ngoc, Tran Thi Thanh Tam, Le Thi Thu Hang, Bui Thanh Thuyet, Le Huu Song, Dong Van Quyen, Juliette Hayer, Anne-Laure Bañuls, Bui Tien Sy
OBJECTIVES: Burkholderia dolosa is a clinically important opportunistic pathogen in inpatients. Here we characterized an extensively drug-resistant and hypervirulent B. dolosa isolate from a patient hospitalized for stroke. METHODS: Resistance to 41 antibiotics was tested with the agar disc diffusion, minimum inhibitory concentration, or broth microdilution method. The complete genome was assembled using short-reads and long-reads and the hybrid de novo assembly method...
February 24, 2024: Journal of Global Antimicrobial Resistance
https://read.qxmd.com/read/38405156/preserved-c-peptide-is-common-and-associated-with-higher-time-in-range-in-chinese-type-1-diabetes
#22
JOURNAL ARTICLE
Wei Liu, Yayu Fang, Xiaoling Cai, Yu Zhu, Mingxia Zhang, Xueyao Han, Juan Li, Sai Yin, Deheng Cai, Jing Chen, Lei Wang, Dawei Shi, Linong Ji
OBJECTIVE: The aim of this study is to determine the residual C-peptide level and to explore the clinical significance of preserved C-peptide secretion in glycemic control in Chinese individuals with type 1 diabetes (T1D). RESEARCH DESIGN AND METHODS: A total of 534 participants with T1D were enrolled and divided into two groups, low-C-peptide group (fasting C-peptide ≤10 pmol/L) and preserved-C-peptide group (fasting C-peptide >10 pmol/L), and clinical factors were compared between the two groups...
2024: Frontiers in Endocrinology
https://read.qxmd.com/read/38404573/tnf%C3%AE-tnfr1-signaling-inhibits-maturation-and-maintains-the-pro-inflammatory-programming-of-monocyte-derived-macrophages-in-murine-chronic-granulomatous-disease
#23
JOURNAL ARTICLE
Sophie L Gibbings, Kelsey C Haist, Elizabeth F Redente, Peter M Henson, Donna L Bratton
INTRODUCTION: Loss of NADPH oxidase activity results in proinflammatory macrophages that contribute to hyperinflammation in Chronic Granulomatous Disease (CGD). Previously, it was shown in a zymosan-induced peritonitis model that gp91phox-/- (CGD) monocyte-derived macrophages (MoMacs) fail to phenotypically mature into pro-resolving MoMacs characteristic of wild type (WT) but retain the ability to do so when placed in the WT milieu. Accordingly, it was hypothesized that soluble factor(s) in the CGD milieu thwart appropriate programming...
2024: Frontiers in Immunology
https://read.qxmd.com/read/38403678/palmitoylation-of-vacuole-membrane-protein-1-promotes-small-extracellular-vesicle-secretion-via-interaction-with-alix-and-influences-intercellular-communication
#24
JOURNAL ARTICLE
Mengyuan Qu, Xinyu Liu, Xiaotong Wang, Zili Li, Liquan Zhou, Honggang Li
BACKGROUND: Small extracellular vesicles (EVs), exemplified by exosomes, mediate intercellular communication by transporting proteins, mRNAs, and miRNAs. Post-translational modifications are involved in controlling small EV secretion process. However, whether palmitoylation regulates small EV secretion, remains largely unexplored. METHODS: Vacuole Membrane Protein 1 (VMP1) was testified to be S-palmitoylated by Palmitoylation assays. VMP1 mutant plasmids were constructed to screen out the exact palmitoylation sites...
February 26, 2024: Cell Communication and Signaling: CCS
https://read.qxmd.com/read/38387829/insights-into-membrane-interactions-and-their-therapeutic-potential
#25
JOURNAL ARTICLE
Calum Upton, Joseph Healey, Alice J Rothnie, Alan D Goddard
Recent research into membrane interactions has uncovered a diverse range of therapeutic opportunities through the bioengineering of human and non-human macromolecules. Although the majority of this research is focussed on fundamental developments, emerging studies are showcasing promising new technologies to combat conditions such as cancer, Alzheimer's and inflammatory and immune-based disease, utilising the alteration of bacteriophage, adenovirus, bacterial toxins, type 6 secretion systems, annexins, mitochondrial antiviral signalling proteins and bacterial nano-syringes...
February 20, 2024: Archives of Biochemistry and Biophysics
https://read.qxmd.com/read/38384466/effects-of-immunogenic-cell-death-inducing-chemotherapeutics-on-the-immune-cell-activation-and-tertiary-lymphoid-structure-formation-in-melanoma
#26
JOURNAL ARTICLE
Hua Zhao, Yu Zhao, Siyuan Zhang, Zhe Wang, Wenwen Yu, Nan Dong, Xuena Yang, Xiying Zhang, Qian Sun, Xishan Hao, Xiubao Ren
BACKGROUND: The infiltration and activation of immune cells in the tumor microenvironment (TIME) affect the prognosis of patients with cancer. Tertiary lymphoid structure (TLS) formation favors tumour- infiltrating-lymphocyte (TIL) recruitment and is regarded as an important indicator of good prognosis associated with immunotherapy in patients with tumors. Chemotherapy is currently one of the most commonly used clinical treatment methods. However, there have been no clear report to explore the effects of different types of chemotherapy on TLS formation in the TIME...
2024: Frontiers in Immunology
https://read.qxmd.com/read/38367471/avian-pathogenic-escherichia-coli-t6ss-effector-protein-hcp2a-causes-mitochondrial-dysfunction-through-interaction-with-letm1-protein-in-df-1-cells
#27
JOURNAL ARTICLE
Liting Lu, Zhao Qi, Zhe Chen, Haiyang Wang, Xiyang Wei, Bingyu Zhao, Zhenyu Wang, Ying Shao, Jian Tu, Xiangjun Song
The type VI secretion system (T6SS) of avian pathogenic Escherichia coli (APEC) can affect the functions of eukaryotic cells by secreting or injecting effectors. Hemolysin co-regulatory protein (Hcp), one of the markers of the T6SS, is both a structural protein and an effector protein of the T6SS. According to previous studies, mitochondria in eukaryotic cells are targeted by pathogenic bacteria. However, little is known about the regulation of mitochondria in eukaryotic host cells by the T6SS effector protein Hcp of APEC...
February 1, 2024: Poultry Science
https://read.qxmd.com/read/38367135/m2-microglia-derived-exosomes-promote-spinal-cord-injury-recovery-in-mice-by-alleviating-a1-astrocyte-activation
#28
JOURNAL ARTICLE
Jing Zhang, Die Hu, Liping Li, Di Qu, Weipeng Shi, Lei Xie, Qi Jiang, Haifeng Li, Tengbo Yu, Chao Qi, Haitao Fu
M2 microglia transplantation has previously demonstrated beneficial effects on spinal cord injury (SCI) by regulating neuroinflammation and enhancing neuronal survival. Exosomes (EXOs), secreted by almost all cell types, embody partial functions and properties of their parent cells. However, the effect of M2 microglia-derived EXOs (M2-EXOs) on SCI recovery and the underlying molecular mechanisms remain unclear. In this study, we isolated M2-EXOs and intravenously introduced them into mice with SCI. Considering the reciprocal communication between microglia and astroglia in both healthy and injured central nervous systems (CNSs), we subsequently focused on the influence of M2-EXOs on astrocyte phenotype regulation...
February 17, 2024: Molecular Neurobiology
https://read.qxmd.com/read/38359344/using-vibrio-natriegens-for-high-yield-production-of-challenging-expression-targets-and-for-protein-perdeuteration
#29
JOURNAL ARTICLE
Natalia Mojica, Flore Kersten, Mateu Montserrat-Canals, G Robb Huhn Iii, Abelone M Tislevoll, Gabriele Cordara, Ken Teter, Ute Krengel
Production of soluble proteins is essential for structure/function studies; however, this usually requires milligram amounts of protein, which can be difficult to obtain with traditional expression systems. Recently, the Gram-negative bacterium Vibrio natriegens emerged as a novel and alternative host platform for production of proteins in high yields. Here, we used a commercial strain derived from V. natriegens (Vmax X2) to produce soluble bacterial and fungal proteins in milligram scale, which we struggled to achieve in Escherichia coli ...
February 15, 2024: Biochemistry
https://read.qxmd.com/read/38355437/genomic-mining-of-vibrio-parahaemolyticus-highlights-prevalence-of-antimicrobial-resistance-genes-and-new-genetic-markers-associated-with-ahpnd-and-tdh%C3%A2-%C3%A2-trh%C3%A2-%C3%A2-genotypes
#30
JOURNAL ARTICLE
Marieke Vandeputte, Sieglinde Coppens, Peter Bossier, Nick Vereecke, Daisy Vanrompay
BACKGROUND: Acute Hepatopancreatic Necrosis Disease (AHPND) causes significant mortality in shrimp aquaculture. The infection is primarily instigated by Vibrio parahaemolyticus (Vp) strains carrying a plasmid encoding the binary toxin PirAB. Yet, comprehension of supplementary virulence factors associated with this relatively recent disease remains limited. Furthermore, the same holds for gastroenteritis in humans caused by other Vp genotypes. Additionally, given the prevalent use of antibiotics to combat bacterial infections, it becomes imperative to illuminate the presence of antimicrobial resistance genes within these bacteria...
February 14, 2024: BMC Genomics
https://read.qxmd.com/read/38353543/decoration-of-burkholderia-hcp1-protein-to-virus-like-particles-as-a-vaccine-delivery-platform
#31
JOURNAL ARTICLE
Nittaya Khakhum, Noe Baruch-Torres, Jacob L Stockton, Itziar Chapartegui-González, Alexander J Badten, Awadalkareem Adam, Tian Wang, Alejandro Huerta-Saquero, Y Whitney Yin, Alfredo G Torres
Virus-like particles (VLPs) are protein-based nanoparticles frequently used as carriers in conjugate vaccine platforms. VLPs have been used to display foreign antigens for vaccination and to deliver immunotherapy against diseases. Hemolysin-coregulated proteins 1 (Hcp1) is a protein component of the Burkholderia type 6 secretion system, which participates in intracellular invasion and dissemination. This protein has been reported as a protective antigen and is used in multiple vaccine candidates with various platforms against melioidosis, a severe infectious disease caused by the intracellular pathogen Burkholderia pseudomallei ...
February 14, 2024: Infection and Immunity
https://read.qxmd.com/read/38348326/single-cell-rna-sequencing-data-generated-from-mouse-adipose-tissue-during-the-development-of-obesity
#32
JOURNAL ARTICLE
Zhimin Lu, Ling Ding, Xuewen Tian, Qinglu Wang
In recent years, the number of obesity has increased rapidly around the world, and it has become a major public health problem endangering global health [1]. Obesity is caused by excessive calorie intake over a long period of time, and high-fat diet (HFD) is one of the important predisposing factors [2], [3], [4]. Adipose tissue (AT) is an important immune and endocrine organ in the body, and plays an important role in the body [5]. Obesity leads to AT dysfunction, AT dilation and cell hypertrophy. Dysfunctional fat cells are the main source of pro-inflammatory cytokines, which aggravate low-grade systemic inflammation and further promote the development of obesity-related diseases [6], [7], [8]...
April 2024: Data in Brief
https://read.qxmd.com/read/38348276/lymphatic-vessels-in-chronic-rhinosinusitis
#33
JOURNAL ARTICLE
Vanessa-Vivien Pesold, Olaf Wendler, Franziska Gröhn, Sarina K Mueller
PURPOSE: The purpose of this study was to analyze the nasal lymphatic system in order to uncover novel factors that might be involved in pathogenesis of chronic rhinosinusitis (CRS) with (CRSwNP) and without nasal polyps (CRSsNP). PATIENTS AND METHODS: Lymphatic vessels (LVs) and macrophages were localized and counted in the inferior and middle turbinate, the uncinate process and the ethmoid of CRSwNP and CRSsNP patients, the NP and the inferior turbinate of controls (n≥6 per group)...
2024: Journal of Inflammation Research
https://read.qxmd.com/read/38345683/dual-release-hydrocortisone-improves-body-composition-and-the-glucometabolic-profile-in-patients-with-secondary-adrenal-insufficiency
#34
JOURNAL ARTICLE
Nanna Thurmann Jørgensen, Victor Brun Boesen, Stina Willemoes Borresen, Thea Christoffersen, Niklas Rye Jørgensen, Peter Plomgaard, Christina Christoffersen, Torquil Watt, Ulla Feldt-Rasmussen, Marianne Klose
PURPOSE: Studies have suggested improved metabolic profiles in patients with adrenal insufficiency treated with dual-release hydrocortisone (DR-HC) compared with conventional hydrocortisone (C-HC). This study investigates the effect of DR-HC compared with C-HC treatment on five health variables: diurnal salivary cortisol/cortisone, body composition, bone health, glucose metabolism, lipids, and blood pressure. METHODS: Prospective study of 27 participants (24 men) with secondary adrenal insufficiency with measurements during stable C-HC and 16 weeks after treatment switch to DR-HC...
February 12, 2024: Endocrine
https://read.qxmd.com/read/38334377/furc-perr-contributes-to-the-regulation-of-peptidoglycan-remodeling-and-intercellular-molecular-transfer-in-the-cyanobacterium-anabaena-sp-strain-pcc-7120
#35
JOURNAL ARTICLE
Cristina Sarasa-Buisan, Mercedes Nieves-Morión, Sergio Arévalo, Richard F Helm, Emma Sevilla, Ignacio Luque, María F Fillat
Microbial extracellular proteins and metabolites provide valuable information concerning how microbes adapt to changing environments. In cyanobacteria, dynamic acclimation strategies involve a variety of regulatory mechanisms, being ferric uptake regulator proteins as key players in this process. In the nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120, FurC (PerR) is a global regulator that modulates the peroxide response and several genes involved in photosynthesis and nitrogen metabolism. To investigate the possible role of FurC in shaping the extracellular environment of Anabaena , the analysis of the extracellular metabolites and proteins of a furC -overexpressing variant was compared to that of the wild-type strain...
February 9, 2024: MBio
https://read.qxmd.com/read/38332152/xanthomonas-immunity-proteins-protect-against-the-cis-toxic-effects-of-their-cognate-t4ss-effectors
#36
JOURNAL ARTICLE
Gabriel U Oka, Diorge P Souza, Germán G Sgro, Cristiane R Guzzo, German Dunger, Chuck S Farah
Many bacteria kill rival species by translocating toxic effectors into target cells. Effectors are often encoded along with cognate immunity proteins that could (i) protect against "friendly-fire" (trans-intoxication) from neighboring sister cells and/or (ii) protect against internal cis-intoxication (suicide). Here, we distinguish between these two mechanisms in the case of the bactericidal Xanthomonas citri Type IV Secretion System (X-T4SS). We use a set of X. citri mutants lacking multiple effector/immunity protein (X-Tfe/X-Tfi) pairs to show that X-Tfis are not absolutely required to protect against trans-intoxication by wild-type cells...
February 8, 2024: EMBO Reports
https://read.qxmd.com/read/38331083/immunomodulation-of-streptozotocin-induced-type-1-diabetes-mellitus-in-mouse-model-by-macrophage-migration-inhibitory-factor-2-mif-2-homologue-of-human-lymphatic-filarial-parasite-wuchereria-bancrofti
#37
JOURNAL ARTICLE
Ishwar Singh, S L Hoti, Nikhil Chauhan, R K Joshi, T S Keshava Prasad, Mohsen Sarikhani, Meenakshi Kaushik, Banappa S Unger, Pankaj Jadav, Prashant Modi
Helminthic parasites modulate the host immune system to ensure a long-lasting asymptomatic form of infection generally, mediated by the secretion of immunomodulatory molecules and one such molecule is a homologue of human host cytokine, Macrophage migratory Inhibitory Factor (hMIF). In this study, we sought to understand the role of homologue of hMIF from the lymphatic filarial parasite, Wuchereria bancrofti (Wba-MIF2), in the immunomodulation of the Streptozotocin-induced Type1 Diabetes Mellitus (T1DM) animal model...
February 6, 2024: Acta Tropica
https://read.qxmd.com/read/38328038/exos-effector-in-pseudomonas-aeruginosa-hyperactive-type-iii-secretion-system-mutant-promotes-enhanced-plasma-membrane-rupture-in-neutrophils
#38
Arianna D Reuven, Bethany W Mwaura, James B Bliska
Pseudomonas aeruginosa is an opportunistic bacterial pathogen responsible for a large percentage of airway infections that cause morbidity and mortality in immunocompromised patients, especially those with cystic fibrosis (CF). One important P. aeruginosa virulence factor is a type III secretion system (T3SS) that translocates effectors into host cells. ExoS is a T3SS effector with ADP ribosyltransferase (ADPRT) activity. The ADPRT activity of ExoS promotes P. aeruginosa virulence by inhibiting phagocytosis and limiting the oxidative burst in neutrophils...
January 25, 2024: bioRxiv
https://read.qxmd.com/read/38319098/genome-analysis-and-hyphal-movement-characterization-of-the-hitchhiker-endohyphal-enterobacter-sp-from-rhizoctonia-solani
#39
JOURNAL ARTICLE
Peiqi Zhang, Jose Huguet-Tapia, Zhao Peng, Sanzhen Liu, Ken Obasa, Anna K Block, Frank F White
Bacterial-fungal interactions are pervasive in the rhizosphere. While an increasing number of endohyphal bacteria have been identified, little is known about their ecology and impact on the associated fungal hosts and the surrounding environment. In this study, we characterized the genome of an Enterobacter sp. Crenshaw (En-Cren), which was isolated from the generalist fungal pathogen Rhizoctonia solani, and examined the genetic potential of the bacterium with regard to the phenotypic traits associated with the fungus...
February 6, 2024: Applied and Environmental Microbiology
https://read.qxmd.com/read/38308618/quorum-sensing-enhances-direct-interspecies-electron-transfer-in-anaerobic-methane-production
#40
JOURNAL ARTICLE
Shunan Zhao, Shaoqing Zhu, Suo Liu, Ge Song, Jing Zhao, Ruiping Liu, Huijuan Liu, Jiuhui Qu
Direct interspecies electron transfer (DIET) provides an innovative way to achieve efficient methanogenesis, and this study proposes a new approach to upregulate the DIET pathway by enhancing quorum sensing (QS). Based on long-term reactor performance, QS enhancement achieved more vigorous methanogenesis with 98.7% COD removal efficiency. In the control system, methanogenesis failure occurred at the accumulated acetate of 7420 mg of COD/L and lowered pH of 6.04, and a much lower COD removal of 41.9% was observed...
February 3, 2024: Environmental Science & Technology
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