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L-selectin

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https://www.readbyqxmd.com/read/29218606/pi3k-is-a-linker-between-l-selectin-and-psgl-1-signaling-to-il-18-transcriptional-activation-at-the-promoter-level
#1
Jixian Luo
L-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) are adhesion molecules which induce similar physiological events. Our previous paper showed that phosphatidylinositol 3-kinase (PI3K) played a crucial role in L-selectin- and PSGL-1-mediated F-actin redistribution and assembly during neutrophil rolling on E-selectin. However, it is not clear whether L-selectin and PSGL-1 induce other similar physiology events by PI3K. Here, we investigated the possibility of PI3K linking the signaling pathways of L-selectin and PSGL-1 to IL-18 transcription...
December 7, 2017: Inflammation
https://www.readbyqxmd.com/read/29197072/induction-of-mast-cell-accumulation-by-chymase-via-an-enzymatic-activity-and-icam-1-dependent-mechanism
#2
Huiyun Zhang, Junling Wang, Ling Wang, Mengmeng Zhan, Shigang Li, Zeman Fang, Ciyan Xu, Yanshan Zheng, Shaoheng He
BACKGROUND AND PURPOSE: Chymase is a unique abundant secretory product of mast cells, and a potent chemoattractant for eosinophils, monocytes and neutrophils, but little is known of its influence on mast cell accumulation. EXPERIMENTAL APPROACH: Using mouse peritoneal inflammation model, cell migration assay and flowcytometry analysis, we investigated role of chymase in recruiting mast cells. KEY RESULTS: The results showed that chymase induced up to 5...
December 1, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29182392/effect-of-oral-administration-of-meloxicam-prior-to-transport-on-inflammatory-mediators-and-leukoctye-function-of-cattle-at-feedlot-arrival
#3
Sarah F Capik, Brad J White, Robert L Larson, Nicholas Van Engen, Natalia Cernicchiaro, Terry J Engelken, Jeffrey Lakritz, Michael A Ballou, Lindsey E Hulbert, Rhonda C Vann, Jeff L Caswell, Gabriella Jacob, Jeffery A Carroll, Johann F Coetzee
OBJECTIVE To investigate the effects of meloxicam administration before long-distance transport on inflammatory mediators and leukocyte function of cattle at feedlot arrival. ANIMALS 60 healthy yearling beef steers. PROCEDURES Single-source steers were assigned to a transported (n = 40) or nontransported (20) group. Then, half of the steers within each group were assigned to receive meloxicam (1 mg/kg, PO) or a lactose placebo (1 bolus/steer, PO). All steers were transported approximately 1,300 km overnight to a feedlot; however, the nontransported group was moved before treatment (meloxicam or placebo) administration and allowed a 17-day acclimation period, whereas the transported group was moved immediately after treatment administration on day -1...
December 2017: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/29163156/role-of-translocator-18-kda-ligands-in-the-activation-of-leukotriene-b4-activated-g-protein-coupled-receptor-and-toll-like-receptor-4-pathways-in-neutrophils
#4
Léonard de Vinci Kanda Kupa, Carine C Drewes, Eric D Barioni, Camila L Neves, Sandra Coccuzzo Sampaio, Sandra H P Farsky
TSPO (Translocator 18 KDa; tryptophan-rich sensory protein oxygen sensor) is a constitutive outer mitochondrial membrane protein overexpressed in inflammatory cells during local or systemic processes. Despite its expression is characterized, role of TSPO in inflammation remains elusive. For this study, we investigated the role of TSPO ligands on neutrophil functions elicited by two different inflammatory pathways. Peritoneal neutrophils were isolated from male Balb-C mice, treated with TSPO ligand diazepam, Ro5-4864 or PK11195 (1,100 or 1000 nM; 2 h) and further stimulated with lipopolysaccharide from Escherichia coli (LPS), a binding for Toll-Like Receptor-4 (TLR4), or leukotriene B4 (LTB4), a G-protein coupled receptor (GPCR) ligand...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29137366/p-but-not-e-or-l-selectin-mediated-rolling-adhesion-persistence-in-hemodynamic-flow-diverges-between-metastatic-and-leukocytic-cells
#5
Erin Elizabeth Edwards, Jaeho Oh, Ananyaveena Anilkumar, Katherine Gayle Birmingham, Susan Napier Thomas
The ability of leukocytic cells to engage selectins via rolling adhesion is critical to inflammation, but selectins are also implicated in mediating metastatic dissemination. Using a microfluidic- and flow-based cell adhesion chromatography experimental and analytical technique, we interrogated the cell-subtype differences in engagement and sustainment of rolling adhesion on P-, E-, and L-selectin-functionalized surfaces in physiological flow. Our results indicate that, particularly at low concentrations of P-selectin, metastatic but not leukocytic cells exhibit reduced rolling adhesion persistence, whereas both cell subtypes exhibited reduced persistence on L-selectin and high persistence on E-selectin, differences not revealed by flow cytometry analysis or reflected in the extent or velocity of rolling adhesion...
October 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/29135655/neutrophil-activation-and-enhanced-release-of-granule-products-in-hiv-tb-immune-reconstitution-inflammatory-syndrome
#6
Justine K Nakiwala, Naomi F Walker, Collin R Diedrich, William Worodria, Graeme Meintjes, Robert J Wilkinson, Harriet Mayanja-Kizza, Robert Colebunders, Luc Kestens, Katalin A Wilkinson, David M Lowe
BACKGROUND: Tuberculosis Immune Reconstitution Inflammatory Syndrome (TB-IRIS) remains incompletely understood. Neutrophils are implicated in tuberculosis pathology but detailed investigations in TB-IRIS are lacking. We sought to further explore the biology of TB-IRIS and in particular the role of neutrophils. SETTING: Two observational, prospective cohort studies in HIV/TB co-infected patients starting antiretroviral therapy, one to analyze gene expression and subsequently one to explore neutrophil biology...
November 6, 2017: Journal of Acquired Immune Deficiency Syndromes: JAIDS
https://www.readbyqxmd.com/read/29115434/n-butanol-extract-from-folium%C3%A2-isatidis-inhibits-the-lipopolysaccharide-induced-downregulation-of-cxcr1-and-cxcr2-on-human-neutrophils
#7
Beibei Wu, Liyin Wang, Lili Jiang, Lili Dong, Fengli Xu, Yili Lu, Jiahui Jin, Zhanyue Wang, Guang Liang, Xiaoou Shan
Neutrophils, immune cells crucial for protecting against invading pathogens, are important in sepsis. Neutrophil migration is regulated by chemokine receptors and their cognate ligands. Our previous study investigated the effect of n‑butanol extract from Folium isatidis on lipopolysaccharide (LPS)‑induced septic shock. The present study stimulated neutrophils with LPS to explore the influence of LPS on cell. Neutrophils were then pretreated with n‑butanol extract from Folium isatidis followed by LPS to examine the effect of this extract on neutrophil chemotaxis...
October 25, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28976305/co-targeting-the-tumor-endothelium-and-p-selectin-expressing-glioblastoma-cells-leads-to-a-remarkable-therapeutic-outcome
#8
Shiran Ferber, Galia Tiram, Ana Sousa-Herves, Anat Eldar-Boock, Adva Krivitsky, Anna Scomparin, Eilam Yeini, Paula Ofek, Dikla Ben-Shushan, Laura Isabel Vossen, Kai Licha, Rachel Grossman, Zvi Ram, Jack Henkin, Eytan Ruppin, Noam Auslander, Rainer Haag, Marcelo Calderón, Ronit Satchi-Fainaro
Glioblastoma is a highly aggressive brain tumor. Current standard-of-care results in a marginal therapeutic outcome, partly due to acquirement of resistance and insufficient blood-brain barrier (BBB) penetration of chemotherapeutics. To circumvent these limitations, we conjugated the chemotherapy paclitaxel (PTX) to a dendritic polyglycerol sulfate (dPGS) nanocarrier. dPGS is able to cross the BBB, bind to P/L-selectins and accumulate selectively in intracranial tumors. We show that dPGS has dual targeting properties, as we found that P-selectin is not only expressed on tumor endothelium but also on glioblastoma cells...
October 4, 2017: ELife
https://www.readbyqxmd.com/read/28942362/calcitriol-treatment-ameliorates-inflammation-and-blistering-in-mouse-models-of-epidermolysis-bullosa-acquisita
#9
Stefan Tukaj, Katja Bieber, Mareike Witte, Saeedeh Ghorbanalipoor, Enno Schmidt, Detlef Zillikens, Ralf J Ludwig, Michael Kasperkiewicz
A link between hypovitaminosis D and development of autoimmune bullous disorders has been recently suggested, but this association has not been experimentally elaborated. Here, the role of vitamin D was investigated in epidermolysis bullosa acquisita (EBA), an anti-type VII collagen autoantibody-induced blistering skin disease. Oral administration of the hormonally active vitamin D metabolite calcitriol ameliorated clinical disease severity and dermal neutrophil infiltration in both an antibody transfer- and immunization-induced EBA mouse model...
September 20, 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28890375/anti-inflammatory-properties-of-glpss58-a-sulfated-polysaccharide-from-ganoderma-lucidum
#10
Kai Zhang, Yanfang Liu, Xiangli Zhao, Qingjiu Tang, Jens Dernedde, Jingsong Zhang, Hua Fan
Sulfated polysaccharides exhibit various biological properties, including anti-coagulant, anti-oxidant, anti-viral, anti-cancer, anti-inflammatory and immune regulatory activities. In the present study, the anti-inflammatory properties of GLPss58, a sulfated polysaccharide from Ganoderma lucidum formed by chemical sulfation, were investigated. We found that GLPss58 inhibited L-selectin/sTyr-sLeX binding significantly, blocked the binding of anti-L-selectin antibodies to L-selectin on the surface of human peripheral blood lymphocytes, and inhibited the secondary lymphoid tissue chemokine-induced chemotactic invasion of HPBLs...
September 7, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28861083/d-lactic-acid-induced-adhesion-of-bovine-neutrophils-onto-endothelial-cells-is-dependent-on-neutrophils-extracellular-traps-formation-and-cd11b-expression
#11
Pablo Alarcón, Carolina Manosalva, Ivan Conejeros, María D Carretta, Tamara Muñoz-Caro, Liliana M R Silva, Anja Taubert, Carlos Hermosilla, María A Hidalgo, Rafael A Burgos
Bovine ruminal acidosis is of economic importance as it contributes to reduced milk and meat production. This phenomenon is mainly attributed to an overload of highly fermentable carbohydrate, resulting in increased d(-) lactic acid levels in serum and plasma. Ruminal acidosis correlates with elevated acute phase proteins in blood, along with neutrophil activation and infiltration into various tissues leading to laminitis and aseptic polysynovitis. Previous studies in bovine neutrophils indicated that d(-) lactic acid decreased expression of L-selectin and increased expression of CD11b to concentrations higher than 6 mM, suggesting a potential role in neutrophil adhesion onto endothelia...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28842197/over-expression-of-a-human-cd62l-ecto-domain-and-a-potential-role-of-rna-pseudoknot-structures-in-recombinant-protein-expression
#12
Matthew Spencer, Nathan Max, Joanna Ireland, Zhongcheng Zou, Ruipeng Wang, Peter Sun
L-selectin (CD62L) is an extracellular protein with a lectin-like domain that mediates rolling adhesion of leukocytes to vascular endothelial cell surfaces. Currently, there are no solved structures for the ectodomain of CD62L, nor of CD62L in complex with its ligand. We have developed a rapid mammalian recombinant protein expression system using an amplifiable glutamine synthase based vector. Here, we further developed and applied this method to express and purify the entire extracellular region of CD62L. This resulted in excess of 20 mg/L yield of recombinant CD62L...
December 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28811304/selectin-catch-bonds-mechanotransduce-integrin-activation-and-neutrophil-arrest-on-inflamed-endothelium-under-shear-flow
#13
Vasilios A Morikis, Shannon Chase, Ted Wun, Elliot L Chaikof, John L Magnani, Scott I Simon
E-selectin extends from the plasma membrane of inflamed endothelium and serves to capture leukocytes from flowing blood via long-lived catch-bonds that support slow leukocyte rolling under shear stress. Its ligands are glycosylated with the tetrasaccharide sialyl Lewis(x) (sLe(x)), which contributes to bond affinity and specificity. E-selectin-mediated rolling transmits signals into neutrophils that trigger activation of high-affinity β2-integrins necessary for transition to shear-resistant adhesion and transendothelial migration...
November 9, 2017: Blood
https://www.readbyqxmd.com/read/28801363/l-selectin-promotes-the-maturation-of-dendritic-cells-via-up-regulation-the-expression-of-tlr4-in-vitro
#14
Zhishuai Ye, Jia Liu, Jie Zheng, Jianing Zhang, Rongchong Huang
The relationship between dendritic cells (DCs) and L-selectin in the progress of atherosclerosis is unclear. Here, we used L-selectin co-cultured with DCs to investigate the effect of L-selectin on the maturation of DCs in vitro Monocytes derived DCs were isolated and cultured from human peripheral blood. After being stimulated with L-selectin and/or its antagonist for 24-48 hours, the feather of cells was observed by the electron microscope. The expression of mature antigens CD1a, CD80, CD83, and CD86 were investigated by flow cytometric analysis (FACS)...
August 2017: Annals of Clinical and Laboratory Science
https://www.readbyqxmd.com/read/28771614/trem-1-snp-rs2234246-regulates-trem-1-protein-and-mrna-levels-and-is-associated-with-plasma-levels-of-l-selectin
#15
Alex-Ander Aldasoro Arguinano, Sébastien Dadé, Maria Stathopoulou, Marc Derive, Ndeye Coumba Ndiaye, Ting Xie, Christine Masson, Sébastien Gibot, Sophie Visvikis-Siest
High levels of TREM-1 are associated with cardiovascular and inflammatory diseases risks and the most recent studies have showed that TREM-1 deletion or blockade is associated with up to 60% reduction of the development of atherosclerosis. So far, it is unknown whether the levels of TREM-1 protein are genetically regulated. Moreover, TREM family receptors have been suggested to regulate the cellular adhesion process. The goal of this study was to investigate whether polymorphisms within TREM-1 are regulating the variants of serum TREM-1 levels and the expression levels of their mRNA...
2017: PloS One
https://www.readbyqxmd.com/read/28749083/evidence-of-bone-marrow-downregulation-in-brain-dead-rats
#16
Laura Menegat, Rafael Simas, Julia M Caliman, Fernando L Zanoni, Jacqueline F Jacysyn, Luiz Fernando F da Silva, Primavera Borelli, Luiz Felipe P Moreira, Paulina Sannomiya
Experimental findings support the evidence of a persistent leucopenia triggered by brain death (BD). This study aimed to investigate leucocyte behaviour in bone marrow and blood after BD in rats. BD was induced using intracranial balloon catheter inflation. Sham-operated (SH) rats were trepanned only. Thereafter bone marrow cells were harvested every six hours from the femoral cavity and used for total and differential counts. They were analysed further by flow cytometry to characterize lymphocyte subsets, granulocyte adhesion molecules expression and apoptosis/necrosis [annexin V/propidium iodide (PI) protocol]...
June 2017: International Journal of Experimental Pathology
https://www.readbyqxmd.com/read/28687005/p-but-not-e-or-l-selectin-mediated-rolling-adhesion-persistence-in-hemodynamic-flow-diverges-between-metastatic-and-leukocytic-cells
#17
Erin Elizabeth Edwards, Jaeho Oh, Ananyaveena Anilkumar, Katherine Gayle Birmingham, Susan Napier Thomas
The ability of leukocytic cells to engage selectins via rolling adhesion is critical to inflammation, but selectins are also implicated in mediating metastatic dissemination. Using a microfluidic- and flow-based cell adhesion chromatography experimental and analytical technique, we interrogated the cell-subtype differences in engagement and sustainment of rolling adhesion on P-, E-, and L-selectin-functionalized surfaces in physiological flow. Our results indicate that, particularly at low concentrations of P-selectin, metastatic but not leukocytic cells exhibit reduced rolling adhesion persistence, whereas both cell subtypes exhibited reduced persistence on L-selectin and high persistence on E-selectin, differences not revealed by flow cytometry analysis or reflected in the extent or velocity of rolling adhesion...
June 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28670688/lack-of-ikaros-cripples-expression-of-foxo1-and-its-targets-in-naive-t-cells
#18
Parul Agnihotri, Nicholas M Robertson, Sarah E Umetsu, Ksenia Arakcheeva, Susan Winandy
Ikaros is a transcription factor that regulates lymphocyte development from the level of the haematopoietic stem cell. Lack of Ikaros reduces the ability of progenitor cells to commit to the T-cell lineage, resulting in reduced numbers of early thymic T-cell progenitors and mature T cells. Mature CD4 T cells that lack Ikaros have defects in proliferation, T helper cell differentiation, cytokine expression and the ability to become anergic. A role for Ikaros in the naive T cell has not yet been identified. The receptors interleukin-7 receptor α (IL-7Rα) and l-selectin are important for ensuring survival and proper homing of naive T cells, respectively...
November 2017: Immunology
https://www.readbyqxmd.com/read/28650114/the-effect-of-adhesives-on-inflammatory-immune-markers-during-renal-injury-healing
#19
José Miguel Lloris-Carsí, Carlos Barrios, Beatriz Prieto-Moure, José Miguel Lloris-Cejalvo, Dolores Cejalvo-Lapeña
Renal injury is common in abdominal trauma. Adhesives and sealants can be used to repair and preserve damaged organs. We describe the effect of three biomaterial treatments (TachoSil, GelitaSpon, and Adhflex) on injured renal tissue. Renal traumatic injuries were experimentally induced in male Wistar rats (n = 90) using a punch. Animals were divided into five groups: (1) sham noninjured (n = 3) and punch injury groups; (2) nontreated (n = 6); (3) TachoSil (n = 27); (4) GelitaSpon (n = 27); and (5) Adhflex (n = 27)...
June 26, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28628637/survival-bacterial-clearance-and-thrombocytopenia-are-improved-in-polymicrobial-sepsis-by-targeting-nuclear-transport-shuttles
#20
Ruth Ann Veach, Yan Liu, Jozef Zienkiewicz, Lukasz S Wylezinski, Kelli L Boyd, James L Wynn, Jacek Hawiger
The rising tide of sepsis, a leading cause of death in the US and globally, is not adequately controlled by current antimicrobial therapies and supportive measures, thereby requiring new adjunctive treatments. Severe microvascular injury and multiple organ failure in sepsis are attributed to a "genomic storm" resulting from changes in microbial and host genomes encoding virulence factors and endogenous inflammatory mediators, respectively. This storm is mediated by stress-responsive transcription factors that are ferried to the nucleus by nuclear transport shuttles importins/karyopherins...
2017: PloS One
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