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L-selectin

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https://www.readbyqxmd.com/read/28976305/co-targeting-the-tumor-endothelium-and-p-selectin-expressing-glioblastoma-cells-leads-to-a-remarkable-therapeutic-outcome
#1
Shiran Ferber, Galia Tiram, Ana Sousa-Herves, Anat Eldar-Boock, Adva Krivitsky, Anna Scomparin, Eilam Yeini, Paula Ofek, Dikla Ben-Shushan, Laura Isabel Vossen, Kai Licha, Rachel Grossman, Zvi Ram, Jack Henkin, Eytan Ruppin, Noam Auslander, Rainer Haag, Marcelo Calderón, Ronit Satchi-Fainaro
Glioblastoma is a highly aggressive brain tumor. Current standard-of-care results in a marginal therapeutic outcome, partly due to acquirement of resistance and insufficient blood-brain barrier (BBB) penetration of chemotherapeutics. To circumvent these limitations, we conjugated the chemotherapy paclitaxel (PTX) to a dendritic polyglycerol sulfate (dPGS) nanocarrier. dPGS is able to cross the BBB, bind to P/L-selectins and accumulate selectively in intracranial tumors. We show that dPGS has dual targeting properties, as we found that P-selectin is not only expressed on tumor endothelium but also on glioblastoma cells...
October 4, 2017: ELife
https://www.readbyqxmd.com/read/28942362/calcitriol-treatment-ameliorates-inflammation-and-blistering-in-mouse-models-of-epidermolysis-bullosa-acquisita
#2
Stefan Tukaj, Katja Bieber, Mareike Witte, Saeedeh Ghorbanalipoor, Enno Schmidt, Detlef Zillikens, Ralf J Ludwig, Michael Kasperkiewicz
A link between hypovitaminosis D and development of autoimmune bullous disorders has been recently suggested, but this association has not been experimentally elaborated. Here, the role of vitamin D was investigated in epidermolysis bullosa acquisita (EBA), an anti-type VII collagen autoantibody-induced blistering skin disease. Oral administration of the hormonally active vitamin D metabolite calcitriol ameliorated clinical disease severity and dermal neutrophil infiltration in both an antibody transfer- and immunization-induced EBA mouse model...
September 20, 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28890375/anti-inflammatory-properties-of-glpss58-a-sulfated-polysaccharide-from-ganoderma-lucidum
#3
Kai Zhang, Yanfang Liu, Xiangli Zhao, Qingjiu Tang, Jens Dernedde, Jingsong Zhang, Hua Fan
Sulfated polysaccharides exhibit various biological properties, including anti-coagulant, anti-oxidant, anti-viral, anti-cancer, anti-inflammatory and immune regulatory activities. In the present study, the anti-inflammatory properties of GLPss58, a sulfated polysaccharide from Ganoderma lucidum formed by chemical sulfation, were investigated. We found that GLPss58 inhibited L-selectin/sTyr-sLeX binding significantly, blocked the binding of anti-L-selectin antibodies to L-selectin on the surface of human peripheral blood lymphocytes, and inhibited the secondary lymphoid tissue chemokine-induced chemotactic invasion of HPBLs...
September 7, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28861083/d-lactic-acid-induced-adhesion-of-bovine-neutrophils-onto-endothelial-cells-is-dependent-on-neutrophils-extracellular-traps-formation-and-cd11b-expression
#4
Pablo Alarcón, Carolina Manosalva, Ivan Conejeros, María D Carretta, Tamara Muñoz-Caro, Liliana M R Silva, Anja Taubert, Carlos Hermosilla, María A Hidalgo, Rafael A Burgos
Bovine ruminal acidosis is of economic importance as it contributes to reduced milk and meat production. This phenomenon is mainly attributed to an overload of highly fermentable carbohydrate, resulting in increased d(-) lactic acid levels in serum and plasma. Ruminal acidosis correlates with elevated acute phase proteins in blood, along with neutrophil activation and infiltration into various tissues leading to laminitis and aseptic polysynovitis. Previous studies in bovine neutrophils indicated that d(-) lactic acid decreased expression of L-selectin and increased expression of CD11b to concentrations higher than 6 mM, suggesting a potential role in neutrophil adhesion onto endothelia...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28842197/over-expression-of-a-human-cd62l-ecto-domain-and-a-potential-role-of-rna-pseudoknot-structures-in-recombinant-protein-expression
#5
Matthew Spencer, Nathan Max, Joanna Ireland, Zhongcheng Zou, Ruipeng Wang, Peter Sun
L-selectin (CD62L) is an extracellular protein with a lectin-like domain that mediates rolling adhesion of leukocytes to vascular endothelial cell surfaces. Currently, there are no solved structures for the ectodomain of CD62L, nor of CD62L in complex with its ligand. We have developed a rapid mammalian recombinant protein expression system using an amplifiable glutamine synthase based vector. Here, we further developed and applied this method to express and purify the entire extracellular region of CD62L. This resulted in excess of 20 mg/L yield of recombinant CD62L...
December 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28811304/selectin-catch-bonds-mechanotransduce-integrin-activation-and-neutrophil-arrest-on-inflamed-endothelium-under-shear-flow
#6
Vasilios A Morikis, Shannon Chase, Ted Wun, Elliot L Chaikof, John L Magnani, Scott I Simon
E-selectin extends from the plasma membrane of inflamed endothelium and serves to capture leukocytes from flowing blood via long-lived catch-bonds that support slow leukocyte rolling under shear stress. Its ligands are glycosylated with the tetrasaccharide sialyl Lewis(x) (sLe(x)), which contributes to bond affinity and specificity. E-selectin mediated rolling transmits signals into neutrophils that triggers activation of high-affinity β2-integrins necessary for transition to shear resistant adhesion and transendothelial migration...
August 15, 2017: Blood
https://www.readbyqxmd.com/read/28801363/l-selectin-promotes-the-maturation-of-dendritic-cells-via-up-regulation-the-expression-of-tlr4-in-vitro
#7
Zhishuai Ye, Jia Liu, Jie Zheng, Jianing Zhang, Rongchong Huang
The relationship between dendritic cells (DCs) and L-selectin in the progress of atherosclerosis is unclear. Here, we used L-selectin co-cultured with DCs to investigate the effect of L-selectin on the maturation of DCs in vitro Monocytes derived DCs were isolated and cultured from human peripheral blood. After being stimulated with L-selectin and/or its antagonist for 24-48 hours, the feather of cells was observed by the electron microscope. The expression of mature antigens CD1a, CD80, CD83, and CD86 were investigated by flow cytometric analysis (FACS)...
August 2017: Annals of Clinical and Laboratory Science
https://www.readbyqxmd.com/read/28771614/trem-1-snp-rs2234246-regulates-trem-1-protein-and-mrna-levels-and-is-associated-with-plasma-levels-of-l-selectin
#8
Alex-Ander Aldasoro Arguinano, Sébastien Dadé, Maria Stathopoulou, Marc Derive, Ndeye Coumba Ndiaye, Ting Xie, Christine Masson, Sébastien Gibot, Sophie Visvikis-Siest
High levels of TREM-1 are associated with cardiovascular and inflammatory diseases risks and the most recent studies have showed that TREM-1 deletion or blockade is associated with up to 60% reduction of the development of atherosclerosis. So far, it is unknown whether the levels of TREM-1 protein are genetically regulated. Moreover, TREM family receptors have been suggested to regulate the cellular adhesion process. The goal of this study was to investigate whether polymorphisms within TREM-1 are regulating the variants of serum TREM-1 levels and the expression levels of their mRNA...
2017: PloS One
https://www.readbyqxmd.com/read/28749083/evidence-of-bone-marrow-downregulation-in-brain-dead-rats
#9
Laura Menegat, Rafael Simas, Julia M Caliman, Fernando L Zanoni, Jacqueline F Jacysyn, Luiz Fernando F da Silva, Primavera Borelli, Luiz Felipe P Moreira, Paulina Sannomiya
Experimental findings support the evidence of a persistent leucopenia triggered by brain death (BD). This study aimed to investigate leucocyte behaviour in bone marrow and blood after BD in rats. BD was induced using intracranial balloon catheter inflation. Sham-operated (SH) rats were trepanned only. Thereafter bone marrow cells were harvested every six hours from the femoral cavity and used for total and differential counts. They were analysed further by flow cytometry to characterize lymphocyte subsets, granulocyte adhesion molecules expression and apoptosis/necrosis [annexin V/propidium iodide (PI) protocol]...
June 2017: International Journal of Experimental Pathology
https://www.readbyqxmd.com/read/28687005/p-but-not-e-or-l-selectin-mediated-rolling-adhesion-persistence-in-hemodynamic-flow-diverges-between-metastatic-and-leukocytic-cells
#10
Erin Elizabeth Edwards, Jaeho Oh, Ananyaveena Anilkumar, Katherine Gayle Birmingham, Susan Napier Thomas
The ability of leukocytic cells to engage selectins via rolling adhesion is critical to inflammation, but selectins are also implicated in mediating metastatic dissemination. Using a microfluidic- and flow-based cell adhesion chromatography experimental and analytical technique, we interrogated the cell-subtype differences in engagement and sustainment of rolling adhesion on P-, E-, and L-selectin-functionalized surfaces in physiological flow. Our results indicate that, particularly at low concentrations of P-selectin, metastatic but not leukocytic cells exhibit reduced rolling adhesion persistence, whereas both cell subtypes exhibited reduced persistence on L-selectin and high persistence on E-selectin, differences not revealed by flow cytometry analysis or reflected in the extent or velocity of rolling adhesion...
June 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28670688/lack-of-ikaros-cripples-expression-of-foxo1-and-its-targets-in-naive-t-cells
#11
Parul Agnihotri, Nicholas M Robertson, Sarah E Umetsu, Ksenia Arakcheeva, Susan Winandy
Ikaros is a transcription factor that regulates lymphocyte development from the level of the haematopoietic stem cell. Lack of Ikaros reduces the ability of progenitor cells to commit to the T-cell lineage, resulting in reduced numbers of early thymic T-cell progenitors and mature T cells. Mature CD4 T cells that lack Ikaros have defects in proliferation, T helper cell differentiation, cytokine expression and the ability to become anergic. A role for Ikaros in the naive T cell has not yet been identified. The receptors interleukin-7 receptor α (IL-7Rα) and l-selectin are important for ensuring survival and proper homing of naive T cells, respectively...
November 2017: Immunology
https://www.readbyqxmd.com/read/28650114/the-effect-of-adhesives-on-inflammatory-immune-markers-during-renal-injury-healing
#12
José Miguel Lloris-Carsí, Carlos Barrios, Beatriz Prieto-Moure, José Miguel Lloris-Cejalvo, Dolores Cejalvo-Lapeña
Renal injury is common in abdominal trauma. Adhesives and sealants can be used to repair and preserve damaged organs. We describe the effect of three biomaterial treatments (TachoSil, GelitaSpon, and Adhflex) on injured renal tissue. Renal traumatic injuries were experimentally induced in male Wistar rats (n = 90) using a punch. Animals were divided into five groups: (1) sham noninjured (n = 3) and punch injury groups; (2) nontreated (n = 6); (3) TachoSil (n = 27); (4) GelitaSpon (n = 27); and (5) Adhflex (n = 27)...
June 26, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28628637/survival-bacterial-clearance-and-thrombocytopenia-are-improved-in-polymicrobial-sepsis-by-targeting-nuclear-transport-shuttles
#13
Ruth Ann Veach, Yan Liu, Jozef Zienkiewicz, Lukasz S Wylezinski, Kelli L Boyd, James L Wynn, Jacek Hawiger
The rising tide of sepsis, a leading cause of death in the US and globally, is not adequately controlled by current antimicrobial therapies and supportive measures, thereby requiring new adjunctive treatments. Severe microvascular injury and multiple organ failure in sepsis are attributed to a "genomic storm" resulting from changes in microbial and host genomes encoding virulence factors and endogenous inflammatory mediators, respectively. This storm is mediated by stress-responsive transcription factors that are ferried to the nucleus by nuclear transport shuttles importins/karyopherins...
2017: PloS One
https://www.readbyqxmd.com/read/28596771/regulation-of-t-cell-trafficking-by-enzymatic-synthesis-of-o-glycans
#14
REVIEW
Samuel J Hobbs, Jeffrey C Nolz
Selectins constitute a family of oligosaccharide binding proteins that play critical roles in regulating the trafficking of leukocytes. In T cells, L-selectin (CD62L) controls the capacity for naive and memory T cells to actively survey peripheral lymph nodes, whereas P- and E-selectin capture activated T cells on inflamed vascular endothelium to initiate extravasation into non-lymphoid tissues. The capacity for T cells to interact with all of these selectins is dependent on the enzymatic synthesis of complex O-glycans, and thus, this protein modification plays an indispensable role in regulating the distribution and homing of both naive and previously activated T cells in vivo...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28595946/the-effects-of-pessimism-on-cell-mediated-immunity-in-rats
#15
Katarzyna Curzytek, Marta Kubera, Ewa Trojan, Kinga Wójcik, Agnieszka Basta-Kaim, Jan Detka, Michael Maes, Rafal Rygula
We used a recently developed ambiguous-cue interpretation (ACI) paradigm to investigate whether 'optimism' and 'pessimism' as behavioural traits may be interrelated with immune functions in rodents. To this aim, in a series of ACI tests (cognitive bias screening, CBS), we identified rats that displayed 'pessimistic' and 'optimistic' traits. We found significant differences in immune biomarkers between 'optimistic' and 'pessimistic' animals. Moreover 'pessimism' was associated with significantly lower relative weight of the spleen and thymus, significantly decreased proliferative activity of splenocytes...
June 5, 2017: Progress in Neuro-psychopharmacology & Biological Psychiatry
https://www.readbyqxmd.com/read/28594849/high-sodium-diet-converts-renal-proteoglycans-into-pro-inflammatory-mediators-in-rats
#16
Ryanne S Hijmans, Pragyi Shrestha, Kwaku A Sarpong, Saleh Yazdani, Rana El Masri, Wilhelmina H A de Jong, Gerjan Navis, Romain R Vivès, Jacob van den Born
BACKGROUND: High dietary sodium aggravates renal disease by affecting blood pressure and by its recently shown pro-inflammatory and pro-fibrotic effects. Moreover, pro-inflammatory modification of renal heparan sulfate (HS) can induce tissue remodeling. We aim to investigate if high sodium intake in normotensive rats converts renal HS into a pro-inflammatory phenotype, able to bind more sodium and orchestrate inflammation, fibrosis and lymphangiogenesis. METHODS: Wistar rats received a normal diet for 4 weeks, or 8% NaCl diet for 2 or 4 weeks...
2017: PloS One
https://www.readbyqxmd.com/read/28551191/a-commercial-immune-modulating-feed-additive-restores-l-selectin-and-ccl5-expression-following-dexamethasone-treatment-of-murine-immune-cells-in-a-myd88-dependent-manner
#17
R J Ortiz-Marty, A Lewandowski, J D Chapman, I Kanevsky-Mullarky
Bovine mastitis costs the dairy industry billions of dollars every year and presents a health challenge in dairy facilities. Immunosuppressive effects of the periparturient period increase the incidence of mastitis. During this time, cattle experience an elevation in circulating cortisol, which reduces polymorphonuclear cell function and ability to clear infection. OmniGen-AF (OMN; Phibro Animal Health, Teaneck, NJ) is an immunomodulatory feed additive that alters gene expression and is used to reduce rates of mastitis...
May 24, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28543181/loss-of-l-selectin-guided-cd8-but-not-cd4-cells-protects-against-ischemia-reperfusion-injury-in-a-steatotic-liver
#18
Vasantha L Kolachala, Sirish Palle, Ming Shen, Alayna Feng, Dmitry Shayakhmetov, Nitika A Gupta
Steatotic liver responds with increased hepatocellular injury when exposed to an ischemic-reperfusion insult. Increasing evidence supports the role of immune cells as key mediators of this injury in a normal (lean) state, but data about their role in a steatotic liver are practically nonexistent. The objective of the current study was to delineate the contribution of specific phenotypes of T cells and adhesion molecules in exacerbated cell death in steatotic liver injury. RNA sequencing was performed on isolated steatotic primary hepatocytes, and T-cell markers were assessed in hepatic lymphocytes after ischemia reperfusion injury (IRI) in high-fat diet (HFD)-fed mice...
October 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/28515724/an-analysis-of-trafficking-receptors-shows-that-cd44-and-p-selectin-glycoprotein-ligand-1-collectively-control-the-migration-of-activated-human-t-cells
#19
Amal J Ali, Ayman F Abuelela, Jasmeen S Merzaban
Selectins guide the traffic of activated T-cells through the blood stream by mediating their tethering and rolling onto inflamed endothelium, in this way acting as beacons to help navigate them to sites of inflammation. Here, we present a comprehensive analysis of E-selectin ligands expressed on activated human T-cells. We identified several novel glycoproteins that function as E-selectin ligands. Specifically, we compared the role of P-selectin glycoprotein ligand-1 (PSGL-1) and CD43, known E-selectin ligands, to CD44, a ligand that has not previously been characterized as an E-selectin ligand on activated human T-cells...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28497779/l-selectin-mechanochemistry-restricts-neutrophil-priming-in-vivo
#20
Zhenghui Liu, Tadayuki Yago, Nan Zhang, Sumith R Panicker, Ying Wang, Longbiao Yao, Padmaja Mehta-D'souza, Lijun Xia, Cheng Zhu, Rodger P McEver
Circulating neutrophils must avoid premature activation to prevent tissue injury. The leukocyte adhesion receptor L-selectin forms bonds with P-selectin glycoprotein ligand-1 (PSGL-1) on other leukocytes and with peripheral node addressin (PNAd) on high endothelial venules. Mechanical forces can strengthen (catch) or weaken (slip) bonds between biological molecules. How these mechanochemical processes influence function in vivo is unexplored. Here we show that mice expressing an L-selectin mutant (N138G) have altered catch bonds and prolonged bond lifetimes at low forces...
May 12, 2017: Nature Communications
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