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L-selectin

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https://www.readbyqxmd.com/read/28811304/selectin-catch-bonds-mechanotransduce-integrin-activation-and-neutrophil-arrest-on-inflamed-endothelium-under-shear-flow
#1
Vasilios A Morikis, Shannon Chase, Ted Wun, Elliot L Chaikof, John L Magnani, Scott I Simon
E-selectin extends from the plasma membrane of inflamed endothelium and serves to capture leukocytes from flowing blood via long-lived catch-bonds that support slow leukocyte rolling under shear stress. Its ligands are glycosylated with the tetrasaccharide sialyl Lewis(x) (sLe(x)), which contributes to bond affinity and specificity. E-selectin mediated rolling transmits signals into neutrophils that triggers activation of high-affinity β2-integrins necessary for transition to shear resistant adhesion and transendothelial migration...
August 15, 2017: Blood
https://www.readbyqxmd.com/read/28801363/l-selectin-promotes-the-maturation-of-dendritic-cells-via-up-regulation-the-expression-of-tlr4-in-vitro
#2
Zhishuai Ye, Jia Liu, Jie Zheng, Jianing Zhang, Rongchong Huang
The relationship between dendritic cells (DCs) and L-selectin in the progress of atherosclerosis is unclear. Here, we used L-selectin co-cultured with DCs to investigate the effect of L-selectin on the maturation of DCs in vitro Monocytes derived DCs were isolated and cultured from human peripheral blood. After being stimulated with L-selectin and/or its antagonist for 24-48 hours, the feather of cells was observed by the electron microscope. The expression of mature antigens CD1a, CD80, CD83, and CD86 were investigated by flow cytometric analysis (FACS)...
August 2017: Annals of Clinical and Laboratory Science
https://www.readbyqxmd.com/read/28771614/trem-1-snp-rs2234246-regulates-trem-1-protein-and-mrna-levels-and-is-associated-with-plasma-levels-of-l-selectin
#3
Alex-Ander Aldasoro Arguinano, Sébastien Dadé, Maria Stathopoulou, Marc Derive, Ndeye Coumba Ndiaye, Ting Xie, Christine Masson, Sébastien Gibot, Sophie Visvikis-Siest
High levels of TREM-1 are associated with cardiovascular and inflammatory diseases risks and the most recent studies have showed that TREM-1 deletion or blockade is associated with up to 60% reduction of the development of atherosclerosis. So far, it is unknown whether the levels of TREM-1 protein are genetically regulated. Moreover, TREM family receptors have been suggested to regulate the cellular adhesion process. The goal of this study was to investigate whether polymorphisms within TREM-1 are regulating the variants of serum TREM-1 levels and the expression levels of their mRNA...
2017: PloS One
https://www.readbyqxmd.com/read/28749083/evidence-of-bone-marrow-downregulation-in-brain-dead-rats
#4
Laura Menegat, Rafael Simas, Julia M Caliman, Fernando L Zanoni, Jacqueline F Jacysyn, Luiz Fernando F da Silva, Primavera Borelli, Luiz Felipe P Moreira, Paulina Sannomiya
Experimental findings support the evidence of a persistent leucopenia triggered by brain death (BD). This study aimed to investigate leucocyte behaviour in bone marrow and blood after BD in rats. BD was induced using intracranial balloon catheter inflation. Sham-operated (SH) rats were trepanned only. Thereafter bone marrow cells were harvested every six hours from the femoral cavity and used for total and differential counts. They were analysed further by flow cytometry to characterize lymphocyte subsets, granulocyte adhesion molecules expression and apoptosis/necrosis [annexin V/propidium iodide (PI) protocol]...
July 27, 2017: International Journal of Experimental Pathology
https://www.readbyqxmd.com/read/28687005/p-but-not-e-or-l-selectin-mediated-rolling-adhesion-persistence-in-hemodynamic-flow-diverges-between-metastatic-and-leukocytic-cells
#5
Erin Elizabeth Edwards, Jaeho Oh, Ananyaveena Anilkumar, Katherine Gayle Birmingham, Susan Napier Thomas
The ability of leukocytic cells to engage selectins via rolling adhesion is critical to inflammation, but selectins are also implicated in mediating metastatic dissemination. Using a microfluidic- and flow-based cell adhesion chromatography experimental and analytical technique, we interrogated the cell-subtype differences in engagement and sustainment of rolling adhesion on P-, E-, and L-selectin-functionalized surfaces in physiological flow. Our results indicate that, particularly at low concentrations of P-selectin, metastatic but not leukocytic cells exhibit reduced rolling adhesion persistence, whereas both cell subtypes exhibited reduced persistence on L-selectin and high persistence on E-selectin, differences not revealed by flow cytometry analysis or reflected in the extent or velocity of rolling adhesion...
June 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28670688/lack-of-ikaros-cripples-expression-of-foxo1-and-its-targets-in-naive-t-cells
#6
Parul Agnihotri, Nicholas M Robertson, Sarah E Umetsu, Ksenia Arakcheeva, Susan Winandy
Ikaros is a transcription factor that regulates lymphocyte development from the level of the hematopoietic stem cell. Lack of Ikaros reduces the ability of progenitor cells to commit to the T cell lineage, resulting in reduced numbers of early thymic T cell progenitors and mature T cells. Mature CD4 T cells that lack Ikaros have defects in proliferation, T helper cell differentiation, cytokine expression and the ability to become anergic. A role for Ikaros in the naïve T cell has not yet been identified. IL-7Rα and L-selectin are receptors important for ensuring survival and proper homing of naive T cells, respectively...
July 3, 2017: Immunology
https://www.readbyqxmd.com/read/28650114/the-effect-of-adhesives-on-inflammatory-immune-markers-during-renal-injury-healing
#7
José Miguel Lloris-Carsí, Carlos Barrios, Beatriz Prieto-Moure, José Miguel Lloris-Cejalvo, Dolores Cejalvo-Lapeña
Renal injury is common in abdominal trauma. Adhesives and sealants can be used to repair and preserve damaged organs. We describe the effect of three biomaterial treatments (TachoSil, GelitaSpon, and Adhflex) on injured renal tissue. Renal traumatic injuries were experimentally induced in male Wistar rats (n = 90) using a punch. Animals were divided into five groups: (1) sham noninjured (n = 3) and punch injury groups; (2) nontreated (n = 6); (3) TachoSil (n = 27); (4) GelitaSpon (n = 27); and (5) Adhflex (n = 27)...
June 26, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28628637/survival-bacterial-clearance-and-thrombocytopenia-are-improved-in-polymicrobial-sepsis-by-targeting-nuclear-transport-shuttles
#8
Ruth Ann Veach, Yan Liu, Jozef Zienkiewicz, Lukasz S Wylezinski, Kelli L Boyd, James L Wynn, Jacek Hawiger
The rising tide of sepsis, a leading cause of death in the US and globally, is not adequately controlled by current antimicrobial therapies and supportive measures, thereby requiring new adjunctive treatments. Severe microvascular injury and multiple organ failure in sepsis are attributed to a "genomic storm" resulting from changes in microbial and host genomes encoding virulence factors and endogenous inflammatory mediators, respectively. This storm is mediated by stress-responsive transcription factors that are ferried to the nucleus by nuclear transport shuttles importins/karyopherins...
2017: PloS One
https://www.readbyqxmd.com/read/28596771/regulation-of-t-cell-trafficking-by-enzymatic-synthesis-of-o-glycans
#9
REVIEW
Samuel J Hobbs, Jeffrey C Nolz
Selectins constitute a family of oligosaccharide binding proteins that play critical roles in regulating the trafficking of leukocytes. In T cells, L-selectin (CD62L) controls the capacity for naive and memory T cells to actively survey peripheral lymph nodes, whereas P- and E-selectin capture activated T cells on inflamed vascular endothelium to initiate extravasation into non-lymphoid tissues. The capacity for T cells to interact with all of these selectins is dependent on the enzymatic synthesis of complex O-glycans, and thus, this protein modification plays an indispensable role in regulating the distribution and homing of both naive and previously activated T cells in vivo...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28595946/the-effects-of-pessimism-on-cell-mediated-immunity-in-rats
#10
Katarzyna Curzytek, Marta Kubera, Ewa Trojan, Kinga Wójcik, Agnieszka Basta-Kaim, Jan Detka, Michael Maes, Rafal Rygula
We used a recently developed ambiguous-cue interpretation (ACI) paradigm to investigate whether 'optimism' and 'pessimism' as behavioural traits may be interrelated with immune functions in rodents. To this aim, in a series of ACI tests (cognitive bias screening, CBS), we identified rats that displayed 'pessimistic' and 'optimistic' traits. We found significant differences in immune biomarkers between 'optimistic' and 'pessimistic' animals. Moreover 'pessimism' was associated with significantly lower relative weight of the spleen and thymus, significantly decreased proliferative activity of splenocytes...
June 5, 2017: Progress in Neuro-psychopharmacology & Biological Psychiatry
https://www.readbyqxmd.com/read/28594849/high-sodium-diet-converts-renal-proteoglycans-into-pro-inflammatory-mediators-in-rats
#11
Ryanne S Hijmans, Pragyi Shrestha, Kwaku A Sarpong, Saleh Yazdani, Rana El Masri, Wilhelmina H A de Jong, Gerjan Navis, Romain R Vivès, Jacob van den Born
BACKGROUND: High dietary sodium aggravates renal disease by affecting blood pressure and by its recently shown pro-inflammatory and pro-fibrotic effects. Moreover, pro-inflammatory modification of renal heparan sulfate (HS) can induce tissue remodeling. We aim to investigate if high sodium intake in normotensive rats converts renal HS into a pro-inflammatory phenotype, able to bind more sodium and orchestrate inflammation, fibrosis and lymphangiogenesis. METHODS: Wistar rats received a normal diet for 4 weeks, or 8% NaCl diet for 2 or 4 weeks...
2017: PloS One
https://www.readbyqxmd.com/read/28551191/a-commercial-immune-modulating-feed-additive-restores-l-selectin-and-ccl5-expression-following-dexamethasone-treatment-of-murine-immune-cells-in-a-myd88-dependent-manner
#12
R J Ortiz-Marty, A Lewandowski, J D Chapman, I Kanevsky-Mullarky
Bovine mastitis costs the dairy industry billions of dollars every year and presents a health challenge in dairy facilities. Immunosuppressive effects of the periparturient period increase the incidence of mastitis. During this time, cattle experience an elevation in circulating cortisol, which reduces polymorphonuclear cell function and ability to clear infection. OmniGen-AF (OMN; Phibro Animal Health, Teaneck, NJ) is an immunomodulatory feed additive that alters gene expression and is used to reduce rates of mastitis...
May 24, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28543181/loss-of-l-selectin-guided-cd8-but-not-cd4-cells-protects-against-ischemia-reperfusion-injury-in-a-steatotic-liver
#13
Vasantha L Kolachala, Sirish Palle, Ming Shen, Alayna Feng, Dmitry Shayakhmetov, Nitika A Gupta
BACKGROUND & AIMS: Steatotic liver responds with increased hepatocellular injury when exposed to an ischemic-reperfusion insult. Increasing evidence supports the role of immune cells as key mediators of this injury in a normal (lean) state, but data about their role in a steatotic liver are practically non-existent. The objective of the current study was to delineate contribution of specific phenotypes of T cells and adhesion molecules in exacerbated cell death in steatotic liver injury...
May 20, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/28515724/an-analysis-of-trafficking-receptors-shows-that-cd44-and-p-selectin-glycoprotein-ligand-1-collectively-control-the-migration-of-activated-human-t-cells
#14
Amal J Ali, Ayman F Abuelela, Jasmeen S Merzaban
Selectins guide the traffic of activated T-cells through the blood stream by mediating their tethering and rolling onto inflamed endothelium, in this way acting as beacons to help navigate them to sites of inflammation. Here, we present a comprehensive analysis of E-selectin ligands expressed on activated human T-cells. We identified several novel glycoproteins that function as E-selectin ligands. Specifically, we compared the role of P-selectin glycoprotein ligand-1 (PSGL-1) and CD43, known E-selectin ligands, to CD44, a ligand that has not previously been characterized as an E-selectin ligand on activated human T-cells...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28497779/l-selectin-mechanochemistry-restricts-neutrophil-priming-in-vivo
#15
Zhenghui Liu, Tadayuki Yago, Nan Zhang, Sumith R Panicker, Ying Wang, Longbiao Yao, Padmaja Mehta-D'souza, Lijun Xia, Cheng Zhu, Rodger P McEver
Circulating neutrophils must avoid premature activation to prevent tissue injury. The leukocyte adhesion receptor L-selectin forms bonds with P-selectin glycoprotein ligand-1 (PSGL-1) on other leukocytes and with peripheral node addressin (PNAd) on high endothelial venules. Mechanical forces can strengthen (catch) or weaken (slip) bonds between biological molecules. How these mechanochemical processes influence function in vivo is unexplored. Here we show that mice expressing an L-selectin mutant (N138G) have altered catch bonds and prolonged bond lifetimes at low forces...
May 12, 2017: Nature Communications
https://www.readbyqxmd.com/read/28489325/reducing-macro-and-microheterogeneity-of-n-glycans-enables-the-crystal-structure-of-the-lectin-and-egf-like-domains-of-human-l-selectin-to-be-solved-at-1-9%C3%A2-%C3%A3-resolution
#16
Stefanie Wedepohl, Jens Dernedde, Ardeschir Vahedi-Faridi, Rudolf Tauber, Wolfram Saenger, Haydar Bulut
L-Selectin, a cell-adhesion receptor on the surface of most leukocytes, contains seven N-glycosylation sites. In order to obtain the crystal structure of human L-selectin, we expressed a shortened version of L-selectin comprising the C-type lectin and EGF-like domains (termed LE) and systematically analysed mutations of the three glycosylation sites (Asn22, Asn66 and Asn139) in order to reduce macroheterogeneity. After we further removed microheterogeneity, we obtained crystals that diffracted X-rays up to 1...
July 4, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28475489/differential-modulation-of-innate-immune-response-by-epinephrine-and-estradiol
#17
Sona Margaryan, Armenuhi Hyusyan, Anush Martirosyan, Shushan Sargsian, Gayane Manukyan
Background Although it is widely accepted that catecholamines and estrogens influence immunity and have consequences for health, their effect on innate immunity (e.g. monocytes and neutrophils) is still not fully investigated. Materials and methods Our study aimed to analyze the production of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, monocyte chemoattractant protein (MCP)-1 and IL-8 by whole blood cells following short-term exposure to epinephrine (Epi) and 17β-estradiol (E2) in the presence or absence of lipopolysaccharide (LPS)...
May 5, 2017: Hormone Molecular Biology and Clinical Investigation
https://www.readbyqxmd.com/read/28464105/effects-of-dexamethasone-treatment-and-respiratory-vaccination-on-rectal-temperature-complete-blood-count-and-functional-capacities-of-neutrophils-in-beef-steers
#18
H D Hughes, J A Carroll, N C Burdick Sanchez, S L Roberts, P R Broadway, N D May, M A Ballou, J T Richeson
The objective of this research was to examine the effects of dexamethasone (DEX) treatment on various aspects of immunity following administration of a multivalent respiratory vaccine, using a model intended to mimic acute versus chronic stress. Angus × Hereford steers ( = 32; 209 ± 8 kg) were stratified by BW and randomly assigned to 1 of 3 treatments: 1) acute stress (ACU), in which 0.5 mg/kg BW DEX was intravenously administered at 1000 h only on d 0; 2) chronic stress (CHR), in which 0.5 mg/kg BW DEX was intravenously administered at 1000 h on d -3 to 0; or 3) control (CON), in which no DEX was administered...
April 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28446179/glycam1-negatively-regulates-monocyte-entry-into-the-optic-nerve-head-and-contributes-to-radiation-based-protection-in-glaucoma
#19
Pete A Williams, Catherine E Braine, Nicole E Foxworth, Kelly E Cochran, Simon W M John
BACKGROUND: We previously reported a profound long-term neuroprotection subsequent to a single radiation-therapy in the DBA/2J mouse model of glaucoma. This neuroprotection prevents entry of monocyte-like immune cells into the optic nerve head during glaucoma. Gene expression studies in radiation-treated mice implicated Glycam1 in this protection. Glycam1 encodes a proteoglycan ligand for L-selectin and is an excellent candidate to modulate immune cell entry into the eye. Here, we experimentally test the hypothesis that radiation-induced over-expression of Glycam1 is a key component of the neuroprotection...
April 26, 2017: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/28434729/effects-of-intrauterine-infusion-of-escherichia-coli-lipopolysaccharide-on-uterine-mrna-gene-expression-and-peripheral-polymorphonuclear-leukocytes-in-jersey-cows-diagnosed-with-purulent-vaginal-discharge
#20
João G N Moraes, Luís G D Mendonça, Paula R B Silva, Alexandre A Scanavez, Klibs N Galvão, Michael A Ballou, Milli Worku, Ricardo C Chebel
The objectives of the current experiment were to investigate the effects of intrauterine treatment of cows with purulent vaginal discharge (PVD) using lipopolysaccharide (LPS) from Escherichia coli on uterine mRNA expression of genes related to inflammatory responses, peripheral polymorphonuclear leukocyte (PMN) function, hematological parameters, and blood concentrations of cortisol, haptoglobin, and progesterone (P4). Jersey cows (n = 3,084) were examined for PVD at 35 ± 6 d postpartum using the Metricheck device (Simcro, Hamilton, New Zealand)...
April 20, 2017: Journal of Dairy Science
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