keyword
MENU ▼
Read by QxMD icon Read
search

L-selectin

keyword
https://www.readbyqxmd.com/read/29430726/comparison-of-l-selectin-blood-level-and-gene-polymorphism-in-tuberculosis-patients-with-healthy-individuals
#1
Peyman Eini, Maria Shirvani, Mehrdad Hajilooi, Farzaneh Esna-Ashari
BACKGROUND: The inflammatory response to Mycobacterium tuberculosis bacilli influences tuberculosis (TB) progression. In this study, we aimed to identify the Phe206Leu polymorphism and serum L-selectin level in TB patients, compared to healthy individuals. METHODS: Ninety patients with a diagnosis of TB and 90 healthy controls were selected in this study. The serum L-selectin level was determined, using ELISA. L-selectin polymorphism was also evaluated using PCR...
February 12, 2018: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/29407321/protective-effect-of-a-novel-technique-for-liver-transplantation-in-the-rat
#2
B Qu, F Yu, G-N Sheng, W Zhang, Y-M Zhang, Y-L Deng, Y Shi, Z-Y Shen
The rat orthotopic liver transplantation model with extremely short anhepatic phase was established to study its protective effect on the recipients and graft. One hundred fifty adult male Wistar rats were randomly divided into three groups: group A (n = 30), using magnetic rings for the suprahepatic vena cava reconstruction; group B (n = 30), using 7/0 Prolene sutures for suprahepatic vena cava running anastomosis as control; and a sham-operated group (n = 30) as a blank control group. The changes in liver enzyme, serum creatinine, endotoxin, and cytokine levels and histopathology were recorded...
January 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/29397822/-itraq-coupled-with-2dlc-ms-ms-to-screen-and-identify-speci-fic-bio-markers-of-t-cell-acute-lymphoblastic-leukemia
#3
Wen-Li Zuo, Run-Hong Yu, Jing-Yu Zhang, Mei Deng, Yu-Feng Liu
OBJECTIVE: To screen and identify potential biomarkers specific for T-cell acute lymphoblastic leukemia (T-ALL). METHODS: Sera were collected from 20 newly diagnosed B-cell acute lymphoblastic leukemia (B-ALL) patients and 20 T-ALL patients. Proteins were extracted, purified and digested with trypsin. All specimens were analyzed by isobaric tags for relative and absolute quantification (iTRAQ) and two-dimensional liquid chromatography-tandem mass spectrometry (2DLC-MS/MS) in a data-dependent mode...
February 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/29385837/verification-of-a-proteomic-biomarker-panel-to-diagnose-minor-stroke-and-transient-ischaemic-attack-phase-1-of-spectra-a-large-scale-translational-study
#4
Andrew M Penn, Maximilian B Bibok, Viera K Saly, Shelagh B Coutts, Mary L Lesperance, Robert F Balshaw, Kristine Votova, Nicole S Croteau, Anurag Trivedi, Angela M Jackson, Janka Hegedus, Evgenia Klourfeld, Amy Y X Yu, Charlotte Zerna, Christoph H Borchers
OBJECTIVE: To derive a plasma biomarker protein panel from a list of 141 candidate proteins which can differentiate transient ischaemic attack (TIA)/minor stroke from non-cerebrovascular ('mimic') conditions in emergency department (ED) settings. DESIGN: Prospective clinical study (#NCT03050099) with up to three timed blood draws no more than 36 hours following symptom onset. Plasma samples analysed by multiple reaction monitoring-mass spectrometry (MRM-MS). PARTICIPANTS: 545 participants suspected of TIA enrolled in the EDs of two urban medical centres...
February 1, 2018: Biomarkers: Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
https://www.readbyqxmd.com/read/29378967/neutrophils-recruited-through-high-endothelial-venules-of-the-lymph-nodes-via-pnad-intercept-disseminating-staphylococcus-aureus
#5
Ania Bogoslowski, Eugene C Butcher, Paul Kubes
Staphylococcus aureus is a skin- and respiratory tract-colonizing bacterium and is the leading cause of community-acquired skin infections. Dissemination of these bacteria into systemic circulation causes bacteremia, which has a high mortality rate. Therefore, understanding the immunologic barriers that prevent dissemination is critical to developing novel treatments. In this study, we demonstrate that an S. aureus breach across skin leads to some migration of the pathogen to the draining lymph node, but no further...
January 29, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29362381/gene-expression-of-human-endometrial-l-selectin-ligand-in-relation-to-the-phases-of-the-natural-menstrual-cycle
#6
Tsung-Hsuan Lai, Fung-Wei Chang, Jun-Jie Lin, Qing-Dong Ling
This study investigates peptide components of L-selectin ligand (LSL) and their gene expressions in human endometrium during the natural menstrual cycle. We recruited 41 endometrial samples from reproductive-aged women with leiomyoma and undergoing hysterectomy and 11 endometrial samples from menopausal women as controls. Immunohistochemistry revealed strong MECA-79 expression from the early through the mid-secretory phase and low expression in menopausal endometrium. Five peptide components of LSL were detected in reproductive and menopausal endometrium by one-step quantitative RT-PCR: podocalyxin, endomucin, nepmucin, GlyCAM-1, and CD34...
January 23, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29353325/a-head-to-tail-view-of-l-selectin-and-its-impact-on-neutrophil-behaviour
#7
REVIEW
Aleksandar Ivetic
L-selectin is a type I transmembrane cell adhesion molecule expressed on most circulating leukocytes, including neutrophils. Engagement of L-selectin with endothelial-derived ligands initiates neutrophil tethering and rolling behaviour along luminal walls of post-capillary venules, constituting the first step of the multi-step adhesion cascade. There is a large body of evidence to suggest that signalling downstream of L-selectin can influence neutrophil behaviour: adhesion, migration and priming. This review will cover aspects of L-selectin form and function and introduce the "triad of L-selectin regulation", highlighting the inextricable links between adhesion, signalling and ectodomain shedding and also highlighting the cytosolic proteins that interconnect them...
January 20, 2018: Cell and Tissue Research
https://www.readbyqxmd.com/read/29350564/role-of-madcam-1-expressing-high-endothelial-venule-like-vessels-in-colitis-induced-in-mice-lacking-sulfotransferases-catalyzing-l-selectin-ligand-biosynthesis
#8
Shulin Low, Jotaro Hirakawa, Hitomi Hoshino, Kenji Uchimura, Hiroto Kawashima, Motohiro Kobayashi
Ulcerative colitis (UC) is a chronic inflammatory disease histologically characterized by diffuse mononuclear cell infiltrates in colonic mucosa. These inflammatory cells are considered to be recruited via high endothelial venule (HEV)-like vessels displaying mucosal addressin cell adhesion molecule 1 (MAdCAM-1), the ligand for α4β7 integrin, and/or peripheral lymph node addressin (PNAd), an L-selectin ligand. 6- O-sulfation of N-acetylglucosamine in the carbohydrate moiety of PNAd is catalyzed exclusively by N-acetylglucosamine-6- O-sulfotransferase 1 (GlcNAc6ST-1) and GlcNAc6ST-2...
January 1, 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/29330473/endothelial-heparan-sulfate-deficiency-reduces-inflammation-and-fibrosis-in-murine-diabetic-nephropathy
#9
Ditmer T Talsma, Kirankumar Katta, Marieke A B Ettema, Berna Kel, Marion Kusche-Gullberg, Moh R Daha, Coen A Stegeman, Jacob van den Born, Lianchun Wang
Inflammation plays a vital role in the development of diabetic nephropathy, but the underlying regulatory mechanisms are only partially understood. Our previous studies demonstrated that, during acute inflammation, endothelial heparan sulfate (HS) contributes to the adhesion and transendothelial migration of leukocytes into perivascular tissues by direct interaction with L-selectin and the presentation of bound chemokines. In the current study, we aimed to assess the role of endothelial HS on chronic renal inflammation and fibrosis in a diabetic nephropathy mouse model...
January 12, 2018: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/29321630/engineered-mesenchymal-stem-cell-sheets-patches-prevents-postoperative-pancreatic-leakage-in-a-rat-model
#10
Seong-Ryong Kim, Hye-Jin Yi, Yu Na Lee, Ji Yoon Park, Robert M Hoffman, Teruo Okano, In Kyong Shim, Song Cheol Kim
Post-operative pancreatic fistula (POPF) following pancreatic resection is a life-threatening surgical complication. Cell sheets were prepared and harvested using temperature-responsive culture dishes and transplanted as patches to seal POPF. Two different mesenchymal stem cell (MSC) sheets were compared in terms of the preventative ability for pancreatic leakage in a rat model. Both rat adipose-derived stem cell (rADSC) and bone marrow-derived stem cell (rBMSC) sheets were transplanted. Those rADSC and rBMSC sheets are created without enzymes and thus maintained their cell-cell junctions and adhesion proteins with intact fibronectin on the basal side, as well as characteristics of MSCs...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29284608/melanocortin-1-receptor-deficiency-promotes-atherosclerosis-in-apolipoprotein-e-mice
#11
Petteri Rinne, James J Kadiri, Mauricio Velasco-Delgado, Salla Nuutinen, Miro Viitala, Maija Hollmén, Martina Rami, Eriika Savontaus, Sabine Steffens
OBJECTIVE: The MC1-R (melanocortin 1 receptor) is expressed by monocytes and macrophages where it mediates anti-inflammatory actions. MC1-R also protects against macrophage foam cell formation primarily by promoting cholesterol efflux through the ABCA1 (ATP-binding cassette transporter subfamily A member 1) and ABCG1 (ATP-binding cassette transporter subfamily G member 1). In this study, we aimed to investigate whether global deficiency in MC1-R signaling affects the development of atherosclerosis...
December 28, 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/29276885/flow-cytometric-bead-sandwich-assay-based-on-split-aptamer
#12
Luyao Shen, Tao Bing, Xiangjun Liu, Junyan Wang, Linlin Wang, Nan Zhang, Dihua Shangguan
A few aptamers still bind their targets after being split into two moieties. Split aptamers have shown great potential in the development of aptameric sensors. However, only a few split aptamers have been generated because of lack of knowledge on the binding structure of their parent aptamers. Here, we report the design of a new split aptamer and a flow cytometric bead sandwich assay using a split aptamer instead of double antibodies. Through DMS footprinting and mutation assay, we figured out the target-binding moiety and the structure-stabilizing moiety of the L-selectin aptamer, Sgc-3b...
December 25, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29218606/pi3k-is-a-linker-between-l-selectin-and-psgl-1-signaling-to-il-18-transcriptional-activation-at-the-promoter-level
#13
Jixian Luo
L-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) are adhesion molecules which induce similar physiological events. Our previous paper showed that phosphatidylinositol 3-kinase (PI3K) played a crucial role in L-selectin- and PSGL-1-mediated F-actin redistribution and assembly during neutrophil rolling on E-selectin. However, it is not clear whether L-selectin and PSGL-1 induce other similar physiology events by PI3K. Here, we investigated the possibility of PI3K linking the signaling pathways of L-selectin and PSGL-1 to IL-18 transcription...
December 7, 2017: Inflammation
https://www.readbyqxmd.com/read/29197072/induction-of-mast-cell-accumulation-by-chymase-via-an-enzymatic-activity-and-icam-1-dependent-mechanism
#14
Huiyun Zhang, Junling Wang, Ling Wang, Mengmeng Zhan, Shigang Li, Zeman Fang, Ciyan Xu, Yanshan Zheng, Shaoheng He
BACKGROUND AND PURPOSE: Chymase is a unique abundant secretory product of mast cells, and a potent chemoattractant for eosinophils, monocytes and neutrophils, but little is known of its influence on mast cell accumulation. EXPERIMENTAL APPROACH: Using mouse peritoneal inflammation model, cell migration assay and flowcytometry analysis, we investigated role of chymase in recruiting mast cells. KEY RESULTS: The results showed that chymase induced up to 5...
December 1, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29182392/effect-of-oral-administration-of-meloxicam-prior-to-transport-on-inflammatory-mediators-and-leukoctye-function-of-cattle-at-feedlot-arrival
#15
Sarah F Capik, Brad J White, Robert L Larson, Nicholas Van Engen, Natalia Cernicchiaro, Terry J Engelken, Jeffrey Lakritz, Michael A Ballou, Lindsey E Hulbert, Rhonda C Vann, Jeff L Caswell, Gabriella Jacob, Jeffery A Carroll, Johann F Coetzee
OBJECTIVE To investigate the effects of meloxicam administration before long-distance transport on inflammatory mediators and leukocyte function of cattle at feedlot arrival. ANIMALS 60 healthy yearling beef steers. PROCEDURES Single-source steers were assigned to a transported (n = 40) or nontransported (20) group. Then, half of the steers within each group were assigned to receive meloxicam (1 mg/kg, PO) or a lactose placebo (1 bolus/steer, PO). All steers were transported approximately 1,300 km overnight to a feedlot; however, the nontransported group was moved before treatment (meloxicam or placebo) administration and allowed a 17-day acclimation period, whereas the transported group was moved immediately after treatment administration on day -1...
December 2017: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/29163156/role-of-translocator-18-kda-ligands-in-the-activation-of-leukotriene-b4-activated-g-protein-coupled-receptor-and-toll-like-receptor-4-pathways-in-neutrophils
#16
Léonard de Vinci Kanda Kupa, Carine C Drewes, Eric D Barioni, Camila L Neves, Sandra Coccuzzo Sampaio, Sandra H P Farsky
TSPO (Translocator 18 KDa; tryptophan-rich sensory protein oxygen sensor) is a constitutive outer mitochondrial membrane protein overexpressed in inflammatory cells during local or systemic processes. Despite its expression is characterized, role of TSPO in inflammation remains elusive. For this study, we investigated the role of TSPO ligands on neutrophil functions elicited by two different inflammatory pathways. Peritoneal neutrophils were isolated from male Balb-C mice, treated with TSPO ligand diazepam, Ro5-4864 or PK11195 (1,100 or 1000 nM; 2 h) and further stimulated with lipopolysaccharide from Escherichia coli (LPS), a binding for Toll-Like Receptor-4 (TLR4), or leukotriene B4 (LTB4), a G-protein coupled receptor (GPCR) ligand...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29137366/p-but-not-e-or-l-selectin-mediated-rolling-adhesion-persistence-in-hemodynamic-flow-diverges-between-metastatic-and-leukocytic-cells
#17
Erin Elizabeth Edwards, Jaeho Oh, Ananyaveena Anilkumar, Katherine Gayle Birmingham, Susan Napier Thomas
The ability of leukocytic cells to engage selectins via rolling adhesion is critical to inflammation, but selectins are also implicated in mediating metastatic dissemination. Using a microfluidic- and flow-based cell adhesion chromatography experimental and analytical technique, we interrogated the cell-subtype differences in engagement and sustainment of rolling adhesion on P-, E-, and L-selectin-functionalized surfaces in physiological flow. Our results indicate that, particularly at low concentrations of P-selectin, metastatic but not leukocytic cells exhibit reduced rolling adhesion persistence, whereas both cell subtypes exhibited reduced persistence on L-selectin and high persistence on E-selectin, differences not revealed by flow cytometry analysis or reflected in the extent or velocity of rolling adhesion...
October 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/29135655/neutrophil-activation-and-enhanced-release-of-granule-products-in-hiv-tb-immune-reconstitution-inflammatory-syndrome
#18
Justine K Nakiwala, Naomi F Walker, Collin R Diedrich, William Worodria, Graeme Meintjes, Robert J Wilkinson, Harriet Mayanja-Kizza, Robert Colebunders, Luc Kestens, Katalin A Wilkinson, David M Lowe
BACKGROUND: Tuberculosis Immune Reconstitution Inflammatory Syndrome (TB-IRIS) remains incompletely understood. Neutrophils are implicated in tuberculosis pathology but detailed investigations in TB-IRIS are lacking. We sought to further explore the biology of TB-IRIS and in particular the role of neutrophils. SETTING: Two observational, prospective cohort studies in HIV/TB co-infected patients starting antiretroviral therapy, one to analyze gene expression and subsequently one to explore neutrophil biology...
November 6, 2017: Journal of Acquired Immune Deficiency Syndromes: JAIDS
https://www.readbyqxmd.com/read/29115434/n-butanol-extract-from-folium%C3%A2-isatidis-inhibits-the-lipopolysaccharide-induced-downregulation-of-cxcr1-and-cxcr2-on-human-neutrophils
#19
Beibei Wu, Liyin Wang, Lili Jiang, Lili Dong, Fengli Xu, Yili Lu, Jiahui Jin, Zhanyue Wang, Guang Liang, Xiaoou Shan
Neutrophils, immune cells crucial for protecting against invading pathogens, are important in sepsis. Neutrophil migration is regulated by chemokine receptors and their cognate ligands. Our previous study investigated the effect of n‑butanol extract from Folium isatidis on lipopolysaccharide (LPS)‑induced septic shock. The present study stimulated neutrophils with LPS to explore the influence of LPS on cell. Neutrophils were then pretreated with n‑butanol extract from Folium isatidis followed by LPS to examine the effect of this extract on neutrophil chemotaxis...
October 25, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28976305/co-targeting-the-tumor-endothelium-and-p-selectin-expressing-glioblastoma-cells-leads-to-a-remarkable-therapeutic-outcome
#20
Shiran Ferber, Galia Tiram, Ana Sousa-Herves, Anat Eldar-Boock, Adva Krivitsky, Anna Scomparin, Eilam Yeini, Paula Ofek, Dikla Ben-Shushan, Laura Isabel Vossen, Kai Licha, Rachel Grossman, Zvi Ram, Jack Henkin, Eytan Ruppin, Noam Auslander, Rainer Haag, Marcelo Calderón, Ronit Satchi-Fainaro
Glioblastoma is a highly aggressive brain tumor. Current standard-of-care results in a marginal therapeutic outcome, partly due to acquirement of resistance and insufficient blood-brain barrier (BBB) penetration of chemotherapeutics. To circumvent these limitations, we conjugated the chemotherapy paclitaxel (PTX) to a dendritic polyglycerol sulfate (dPGS) nanocarrier. dPGS is able to cross the BBB, bind to P/L-selectins and accumulate selectively in intracranial tumors. We show that dPGS has dual targeting properties, as we found that P-selectin is not only expressed on tumor endothelium but also on glioblastoma cells...
October 4, 2017: ELife
keyword
keyword
20084
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"