Meredith E Fay, Oluwamayokun Oshinowo, Elizabeth Iffrig, Kirby S Fibben, Christina Caruso, Scott Hansen, Jamie O Musick, José M Valdez, Sally S Azer, Robert G Mannino, Hyoann Choi, Dan Y Zhang, Evelyn K Williams, Erica N Evans, Celeste K Kanne, Melissa L Kemp, Vivien A Sheehan, Marcus A Carden, Carolyn M Bennett, David K Wood, Wilbur A Lam
While microscopy-based cellular assays, including microfluidics, have significantly advanced over the last several decades, there has not been concurrent development of widely-accessible techniques to analyze time-dependent microscopy data incorporating phenomena such as fluid flow and dynamic cell adhesion. As such, experimentalists typically rely on error-prone and time-consuming manual analysis, resulting in lost resolution and missed opportunities for innovative metrics. We present a user-adaptable toolkit packaged into the open-source, standalone Interactive Cellular assay Labeled Observation and Tracking Software (iCLOTS)...
August 18, 2023: Nature Communications