keyword
MENU ▼
Read by QxMD icon Read
search

OmpK36

keyword
https://www.readbyqxmd.com/read/27788691/outbreak-of-kpc-3-producing-st15-and-st348-klebsiella-pneumoniae-in-a-portuguese-hospital
#1
D Vubil, R Figueiredo, T Reis, C Canha, L Boaventura, G J DA Silva
To date, only a few sporadic cases of infections due to Klebsiella pneumoniae carbapenemase (KPC) producers have been reported in Portugal. Here, we report for the first time an outbreak of K. pneumoniae KPC-3 producers in a tertiary-care hospital during 2013. Twenty-seven ertapenem-resistant K. pneumoniae were identified in patients at a tertiary-care hospital during 2013 isolated predominantly from urine (48·1%) and blood (25·9%) cultures. All isolates were highly resistant to β-lactam antibiotics and most showed intermediate resistance to imipenem...
October 28, 2016: Epidemiology and Infection
https://www.readbyqxmd.com/read/27645385/klebsiella-pneumoniae-major-porins-ompk35-and-ompk36-allow-more-efficient-diffusion-of-%C3%AE-lactams-than-their-escherichia-coli-homologs-ompf-and-ompc
#2
Etsuko Sugawara, Seiji Kojima, Hiroshi Nikaido
: Klebsiella pneumoniae, one of the most important nosocomial pathogens, is becoming a major problem in health care because of its resistance to multiple antibiotics, including cephalosporins of the latest generation and, more recently, even carbapenems. This is largely due to the spread of plasmid-encoded extended-spectrum β-lactamases. However, antimicrobial agents must first penetrate the outer membrane barrier in order to reach their targets, and hydrophilic and charged β-lactams presumably diffuse through the porin channels...
December 1, 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/27542822/molecular-epidemiology-of-extensively-drug-resistant-klebsiella-pneumoniae-outbreak-in-wenzhou-southern-china
#3
Jia Du, Jianming Cao, Lizhen Shen, Wenzi Bi, Xiaoxiao Zhang, Haiyang Liu, Hong Lu, Tieli Zhou
The emergence of extensively drug-resistant (XDR) Klebsiella pneumoniae has become a major challenge worldwide. In this study, we characterized the phenotypes and genetic features of nine XDR K. pneumoniae isolates from an integrated hospital in Zhejiang province, China, from September to October 2014. These XDR K. pneumoniae possessed at least five resistance determinants which contribute to highly resistant to β-lactam, β-lactam/inhibitor combinations, aminoglycosides, quinolones, carbapenems, chloroamphenicol and fosfomycin...
October 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27216068/frequency-of-bkc-1-producing-klebsiella-species-isolates
#4
Willames M B S Martins, Adriana G Nicoletti, Silvia R Santos, Jorge L M Sampaio, Ana C Gales
BKC-1 is a new class A serine carbapenemase that was recently identified in Klebsiella pneumoniae clinical isolates. The principal objective of this study was to evaluate the frequency of blaBKC-1 by testing a collection of Klebsiella isolates. Only 2 of 635 Klebsiella isolates (0.3%) carried blaBKC-1 The two BKC-1-producing isolates belonged to clonal complex 442 and possessed identical pulsed-field gel electrophoresis patterns. The blaBKC-1 gene was inserted into a 10-kb plasmid that was identical to the previously reported plasmid, p60136...
August 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27206024/molecular-epidemiology-of-carbapenem-resistant-klebsiella-pneumoniae-in-greece
#5
Theodoros Karampatakis, Charalampos Antachopoulos, Elias Iosifidis, Athanassios Tsakris, Emmanuel Roilides
Hospital infections caused by carbapenem-resistant Klebsiella pneumoniae (CRKP) constitute a worldwide problem associated with high rates of treatment failure and mortality. In Greece, CRKP have emerged in 2002 due to VIM carbapenemase production and later due to KPC, NDM and OXA-48-like carbapenemases that have become endemic. The molecular epidemiology of CRKP strains is dynamic, as antibiotic consumption and worldwide traveling are strongly associated with global spread of CRKP isolates. Lately, porin defects, such as disruption of OmpK35 and production of OmpK36 variant, have also contributed to carbapenem resistance...
June 2016: Future Microbiology
https://www.readbyqxmd.com/read/27041106/high-level-carbapenem-resistant-oxa-48-producing-klebsiella-pneumoniae-with-a-novel-ompk36-variant-and-low-level-carbapenem-resistant-non-porin-deficient-oxa-181-producing-escherichia-coli-from-thailand
#6
Kamonwan Lunha, Aroonwadee Chanawong, Aroonlug Lulitanond, Chotechana Wilailuckana, Nicha Charoensri, Lumyai Wonglakorn, Pimjai Saenjamla, Prajuab Chaimanee, Sunpetch Angkititrakul, Ploenchan Chetchotisakd
Five blaOXA-48-like-carrying Enterobacteriaceae isolates collected from two Thai patients in December 2012 were characterized. Three Klebsiella pneumoniae isolates giving two different pulsed-field gel electrophoresis patterns and sequence types (ST11 and ST37) from patient 1 harbored blaOXA-48 locating on Tn1999.2, whereas two Escherichia coli isolates with the same pulsotype and ST5 from Patient 2 carried ISEcp1-associated blaOXA-181. One K. pneumoniae strain had blaSHV-12, blaDHA-1, qnrB, and qnrS, while another strain harbored blaCTX-M-15, qnrS and aac(6')-Ib-cr...
June 2016: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27002853/risk-factors-for-kpc-producing-klebsiella-pneumoniae-watch-out-for-surgery
#7
Kesia Esther da Silva, Wirlaine Glauce Maciel, Flávia Patussi Correia Sacchi, Cecilia Godoy Carvalhaes, Fernanda Rodrigues-Costa, Ana Carolina Ramos da Silva, Mariana Garcia Croda, Fábio Juliano Negrão, Julio Croda, Ana Cristina Gales, Simone Simionatto
This study describes the molecular characteristics and risk factors associated with carbapenem-resistant Klebsiella pneumoniae strains. Risk factors associated with KPC-producing K. pneumoniae strains were investigated in this case-control study from May 2011 to May 2013. Bacterial identification was performed by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS). Antimicrobial susceptibility was determined by broth microdilution. Carbapenemase production was assessed by both modified Hodge test (MHT) and ertapenem hydrolysis using MALDI-TOF MS...
June 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/26912748/predictability-of-phenotype-in-relation-to-common-%C3%AE-lactam-resistance-mechanisms-in-escherichia-coli-and-klebsiella-pneumoniae
#8
Alex Agyekum, Alicia Fajardo-Lubián, Xiaoman Ai, Andrew N Ginn, Zhiyong Zong, Xuejun Guo, John Turnidge, Sally R Partridge, Jonathan R Iredell
The minimal concentration of antibiotic required to inhibit the growth of different isolates of a given species with no acquired resistance mechanisms has a normal distribution. We have previously shown that the presence or absence of transmissible antibiotic resistance genes has excellent predictive power for phenotype. In this study, we analyzed the distribution of six β-lactam antibiotic susceptibility phenotypes associated with commonly acquired resistance genes in Enterobacteriaceae in Sydney, Australia...
May 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/26872343/whole-genome-sequence-of-klebsiella-pneumoniae-u25-a-hypermucoviscous-multidrug-resistant-biofilm-producing-isolate-from-india
#9
Zumaana Rafiq, Nithin Sam, Rama Vaidyanathan
Klebsiella pneumoniae U25 is a multidrug resistant strain isolated from a tertiary care hospital in Chennai, India. Here, we report the complete annotated genome sequence of strain U25 obtained using PacBio RSII. This is the first report of the whole genome of K. pneumoniaespecies from Chennai. It consists of a single circular chromosome of size 5,491,870-bp and two plasmids of size 211,813 and 172,619-bp. The genes associated with multidrug resistance were identified. The chromosome of U25 was found to have eight antibiotic resistant genes [blaOXA-1,blaSHV-28, aac(6')1b-cr,catB3, oqxAB, dfrA1]...
February 2016: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/26407326/genetic-characterization-of-carbapenem-resistant-enterobacteriaceae-and-the-spread-of-carbapenem-resistant-klebsiella-pneumonia-st340-at-a-university-hospital-in-thailand
#10
Thidarat Netikul, Pattarachai Kiratisin
Carbapenem-resistant Enterobacteriaceae (CRE) has increasingly spread worldwide in the past decade. The prevalence and characteristics of CRE in Thailand are unknown. In this study, we conducted a 2-year surveillance of CRE among 12,741 clinical isolates of Enterobacteriaceae at the largest university hospital in Thailand with molecular characterization of beta-lactamase (bla) genes, including carbapenemase genes. The CRE prevalence was 1.4%. blaKPC-13 and blaIMP-14a were the only carbapenemase genes detected among these CRE isolates...
2015: PloS One
https://www.readbyqxmd.com/read/26300858/rapid-detection-of-porins-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#11
Yan-Yan Hu, Jia-Chang Cai, Hong-Wei Zhou, Rong Zhang, Gong-Xiang Chen
The rapid and cost-efficient determination of carbapenem resistance is an important prerequisite for the choice of an adequate antibiotic therapy. A MALDI-TOF MS-based assay was set up to detect porins in the current study. A loss of the components of porin alone such as OmpK35/OmpK36 or together with the production of carbapenemases will augment the carbapenem resistance. Ten strains of Escherichia coli and eight strains of Klebsiella pneumoniae were conducted for both sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and MALDI-TOF MS analysis...
2015: Frontiers in Microbiology
https://www.readbyqxmd.com/read/26224840/allocation-of-klebsiella-pneumoniae-bloodstream-isolates-into-four-distinct-groups-by-ompk36-typing-in-a-taiwanese-university-hospital
#12
Jing-Jou Yan, Po-Xing Zheng, Ming-Cheng Wang, Shu-Huei Tsai, Li-Rong Wang, Jiunn-Jong Wu
The OmpK36 porin plays a role in carbapenem resistance and may contribute to bacterial virulence in Klebsiella pneumoniae. This study aimed to investigate the characteristics of different groups of K. pneumoniae separated by ompK36 typing. Among 226 nonduplicate K. pneumoniae bloodstream isolates collected at a Taiwanese hospital in 2011, four ompK36 types, designated types A, B, C, and D, were identified by PCR in 61, 28, 100, and 36 isolates, respectively; 1 isolate was untypeable. Statistical analysis showed significantly higher rates of antimicrobial resistance (all tested antibiotics except meropenem), extended-spectrum β-lactamases or DHA-1 (47...
October 2015: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/26169413/effects-of-klebsiella-pneumoniae-carbapenemase-subtypes-extended-spectrum-%C3%AE-lactamases-and-porin-mutations-on-the-in-vitro-activity-of-ceftazidime-avibactam-against-carbapenem-resistant-k-pneumoniae
#13
Ryan K Shields, Cornelius J Clancy, Binghua Hao, Liang Chen, Ellen G Press, Nicole M Iovine, Barry N Kreiswirth, M Hong Nguyen
Avibactam is a novel β-lactamase inhibitor with affinity for Klebsiella pneumoniae carbapenemases (KPCs). In combination with ceftazidime, the agent demonstrates activity against KPC-producing K. pneumoniae (KPC-Kp). KPC-Kp strains are genetically diverse and harbor multiple resistance determinants, including defects in outer membrane proteins and extended-spectrum β-lactamases (ESBLs). Mutations in porin gene ompK36 confer high-level carbapenem resistance to KPC-Kp strains. Whether specific mechanisms of antimicrobial resistance also influence the activity of ceftazidime-avibactam is unknown...
September 2015: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/26067824/three-dimensional-checkerboard-synergy-analysis-of-colistin-meropenem-tigecycline-against-multidrug-resistant-clinical-klebsiella-pneumonia-isolates
#14
Claudia Stein, Oliwia Makarewicz, Jürgen A Bohnert, Yvonne Pfeifer, Miriam Kesselmeier, Stefan Hagel, Mathias W Pletz
The spread of carbapenem-non-susceptible Klebsiella pneumoniae strains bearing different resistance determinants is a rising problem worldwide. Especially infections with KPC (Klebsiella pneumoniae carbapenemase) - producers are associated with high mortality rates due to limited treatment options. Recent clinical studies of KPC-blood stream infections revealed that colistin-based combination therapy with a carbapenem and/or tigecycline was associated with significantly decreased mortality rates when compared to colistin monotherapy...
2015: PloS One
https://www.readbyqxmd.com/read/26064923/in-vitro-activity-of-imipenem-and-colistin-against-a-carbapenem-resistant-klebsiella-pneumoniae-isolate-coproducing-shv-31-cmy-2-and-dha-1
#15
Hung-Jen Tang, Yee-Huang Ku, Mei-Feng Lee, Yin-Ching Chuang, Wen-Liang Yu
We investigated the synergism of colistin and imipenem against a multidrug-resistant K. pneumoniae isolate which was recovered from a severe hip infection. PCR and DNA sequencing were used to characterize the outer membrane porin genes and the resistance genes mediating the common β-lactamases and carbapenemases. Synergism was evaluated by time-kill studies. The bla SHV-31, bla CMY-2, and bla DHA-1 were detected. Outer membrane porin genes analysis revealed loss of ompK36 and frame-shift mutation of ompK35...
2015: BioMed Research International
https://www.readbyqxmd.com/read/26033723/activity-of-meropenem-combined-with-rpx7009-a-novel-%C3%AE-lactamase-inhibitor-against-gram-negative-clinical-isolates-in-new-york-city
#16
Amabel Lapuebla, Marie Abdallah, Olawole Olafisoye, Christopher Cortes, Carl Urban, John Quale, David Landman
Multidrug-resistant Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae are endemic to hospitals in New York City and other regions. RPX7009 is a novel β-lactamase inhibitor with activity against serine carbapenemases. We tested the activity of meropenem plus RPX7009 against 4,500 recent Gram-negative clinical isolates from 11 New York City hospitals. The meropenem-RPX7009 combination was found to have excellent in vitro activity against Escherichia coli, K. pneumoniae, and Enterobacter spp...
August 2015: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/26014931/activity-of-imipenem-with-relebactam-against-gram-negative-pathogens-from-new-york-city
#17
Amabel Lapuebla, Marie Abdallah, Olawole Olafisoye, Christopher Cortes, Carl Urban, David Landman, John Quale
Imipenem with relebactam was active against Escherichia coli, Klebsiella pneumoniae, and Enterobacter spp., including K. pneumoniae carbapenemase (KPC)-producing isolates. Loss of OmpK36 in KPC-producing K. pneumoniae isolates affected the susceptibility of this combination. Enhanced activity was evident against Pseudomonas aeruginosa, including isolates with depressed oprD and increased ampC expression. However, the addition of relebactam to imipenem did not provide added benefit against Acinetobacter baumannii...
August 2015: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/25945596/the-role-of-ompk35-ompk36-porins-and-production-of-%C3%AE-lactamases-on-imipenem-susceptibility-in-klebsiella-pneumoniae-clinical-isolates-cairo-egypt
#18
Mona Wassef, Mona Abdelhaleim, Eiman AbdulRahman, Doaa Ghaith
BACKGROUND: OmpK35 and OmpK36 are the major outer membrane porins of Klebsiella pneumoniae. We aimed to study the effect of combined porin loss and production of extended-spectrum β-lactamases (ESBLs) on imipenem susceptibility among K. pneumoniae clinical isolates. MATERIALS AND METHODS: This study included 91 suspected ESBL-producing K. pneumoniae clinical isolates, isolated from different patient specimens at the Cairo University hospital from January to June 2010...
December 2015: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/25914088/molecular-epidemiology-of-carbapenemase-producing-klebsiella-pneumoniae-in-a-hospital-in-madrid-successful-establishment-of-an-oxa-48-st11-clone
#19
Patricia Brañas, Jennifer Villa, Esther Viedma, Jesús Mingorance, M Angeles Orellana, Fernando Chaves
Here we report a retrospective clinical and molecular study conducted in a tertiary care facility in southern Madrid, Spain, from January 2009 to February 2014 to investigate the epidemiology of carbapenemase-producing Klebsiella pneumoniae (CPKp). Carbapenemase genes were identified in 97 non-duplicate K. pneumoniae isolates, including 59 harbouring blaOXA-48, 37 harbouring blaVIM-1 and 1 harbouring blaKPC-2. Pulsed-field gel electrophoresis (PFGE) analysis verified the presence of 20 different clonal types, whilst multilocus sequence typing (MLST) assigned the isolates to eight sequence types (STs)...
July 2015: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/25834737/associations-of-the-major-international-high-risk-resistant-clones-and-virulent-clones-with-specific-ompk36-allele-groups-in-klebsiella-pneumoniae-in-taiwan
#20
J J Yan, M C Wang, P X Zheng, L H Tsai, J J Wu
This study was conducted to investigate the association between ompK36 variants and international high-risk clones in Klebsiella pneumoniae. Fifty-nine sequence types (STs) divided into four ompK36 allele groups (groups A to D) were identified among 185 K. pneumoniae isolates. The major high-risk clones (29 ST11, 13 ST15, 7 ST37 and 1 ST147 isolates) were assigned to group A, while 6 STs (15 ST23, 2 ST65, 3 ST86, 1 ST163, 1 ST373 and 2 ST375 isolates) associated with pyogenic liver abscess were assigned to group C...
May 2015: New Microbes and New Infections
keyword
keyword
20044
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"