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https://www.readbyqxmd.com/read/29309834/recombinant-hsp33-and-ompc-protein-can-serve-as-promising-divalent-vaccine-with-protection-against-vibrio-anguillarum-and-edwardsiella-tarda-in-flounder-paralichthys-olivaceus
#1
Jing Xing, Pengwei Li, Xiaoqian Tang, Wenbin Zhan
Vibrio anguillarum and Edwardsiella tarda are severe aquaculture pathogens shared similar epidemiological characteristic and susceptible to flounder (Paralichthys olivaceus). In our previous studies, recombinant(r) protein heat shock protein 33 (rHsp33) from V. anguillarum and outer membrane protein C (rOmpC) from E. tarda were proved to have protection against V. anguillarum and E. tarda, respectively. In this paper, the cross protection of rHsp33 against E. tarda and rOmpC against V. anguillarum, and the protection of divalent vaccine candidate (rHsp33 + rOmpC, rHC) against both V...
January 5, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29307192/enrofloxacin-permeation-pathways-across-the-porin-ompc
#2
Jigneshkumar Dahyabhai Prajapati, Carlos José Fernández Solano, Mathias Winterhalter, Ulrich Kleinekathöfer
In Gram-negative bacteria, the lack or quenching of antibiotics translocation across the outer membrane is one of the main factors for acquiring antibiotic resistance. An atomic level comprehension of the key features governing the transport of drugs by outer membrane protein channels will be very helpful in developing the next generation of antibiotics. In a previous study [J. D. Prajapati et al, J. Chem. Theory Comput. 2017, 13, 4553--4566], we characterized the diffusion pathway of the ciprofloxacin molecule through the outer membrane porin OmpC of E...
January 6, 2018: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29211019/dual-regulation-of-the-small-rna-micc-and-the-quiescent-porin-ompn-in-response-to-antibiotic-stress-in-escherichia-coli
#3
Sushovan Dam, Jean-Marie Pagès, Muriel Masi
Antibiotic resistant Gram-negative bacteria are a serious threat for public health. The permeation of antibiotics through their outer membrane is largely dependent on porin, changes in which cause reduced drug uptake and efficacy. Escherichia coli produces two major porins, OmpF and OmpC. MicF and MicC are small non-coding RNAs (sRNAs) that modulate the expression of OmpF and OmpC, respectively. In this work, we investigated factors that lead to increased production of MicC. micC promoter region was fused to lacZ, and the reporter plasmid was transformed into E...
December 6, 2017: Antibiotics
https://www.readbyqxmd.com/read/29185080/manganese-and-cobalt-recovery-by-surface-display-of-metal-binding-peptide-on-various-loops-of-ompc-in-escherichia-coli
#4
Murali Kannan Maruthamuthu, Vidhya Selvamani, Saravanan Prabhu Nadarajan, Hyungdon Yun, You-Kwan Oh, Gyeong Tae Eom, Soon Ho Hong
In a cell-surface display (CSD) system, successful display of a protein or peptide is highly dependent on the anchoring motif and the position of the display in that anchoring motif. In this study, a recombinant bacterial CSD system for manganese (Mn) and cobalt (Co) recovery was developed by employing OmpC as an anchoring motif on three different external loops. A portion of Cap43 protein (TRSRSHTSEG)3 was employed as a manganese and cobalt binding peptide (MCBP), which was fused with OmpC at three different external loops...
November 28, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/29180369/membrane-vesicles-derived-from-bordetella-bronchiseptica-active-constituent-of-a-new-vaccine-against-infections-caused-by-this-pathogen
#5
D Bottero, M E Zurita, M E Gaillard, E Bartel, C Vercellini, D Hozbor
Bordetella bronchiseptica, a Gram-negative bacterium, causes chronic respiratory-tract infections in a wide variety of mammalian hosts-humans included, albeit rarely. We recently designed B. pertussis and B. parapertussis experimental vaccines based on outer-membrane vesicles derived from each pathogen and obtained protection against the respective infections in mice. Here, we demonstrate that outer-membrane vesicles derived from virulent-phase B. bronchiseptica (OMVBbvir+) protect mice against sublethal infection from different B...
November 27, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29164072/a-new-strain-collection-for-improved-expression-of-outer-membrane-proteins
#6
Ina Meuskens, Marcin Michalik, Nandini Chauhan, Dirk Linke, Jack C Leo
Almost all integral membrane proteins found in the outer membranes of Gram-negative bacteria belong to the transmembrane β-barrel family. These proteins are not only important for nutrient uptake and homeostasis, but are also involved in such processes as adhesion, protein secretion, biofilm formation, and virulence. As surface exposed molecules, outer membrane β-barrel proteins are also potential drug and vaccine targets. High production levels of heterologously expressed proteins are desirable for biochemical and especially structural studies, but over-expression and subsequent purification of membrane proteins, including outer membrane proteins, can be challenging...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29055550/the-phylogenetic-group-antimicrobial-susceptibility-and-virulence-genes-of-escherichia-coli-from-clinical-bovine-mastitis
#7
Dexian Zhang, Zehui Zhang, Chengcheng Huang, Xiang Gao, Zhuang Wang, Yaochuan Liu, Chunlian Tian, Wei Hong, Shengli Niu, Mingchun Liu
Bovine mastitis is still a central problem on dairy farms despite control programs, and Escherichia coli is a crucial pathogen during the development of bovine mastitis. The virulence genes, antimicrobial susceptibility, and mortality of mice infected with different E. coli isolates from bovine mastitis were determined in this study. According to the presence of the specific genes chuA, yjaA, and TspE4.C2, these isolates mainly belonged to 2 different groups: group A (47/79) and group B1 (22/79). The ompC gene was detected in all the isolates, followed by fimH (89...
October 18, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/29038023/the-impact-of-length-variations-in-the-l2-loop-on-the-structure-and-thermal-stability-of-non-specific-porins-the-case-of-ompcs-from-the-yersinia-pseudotuberculosis-complex
#8
T Solov'eva, G Likhatskaya, V Khomenko, K Guzev, N Kim, E Bystritskaya, O Novikova, A Stenkova, A Rakin, M Isaeva
Porins are integral proteins of the outer membranes of gram-negative bacteria. In membranes, they exist as homotrimers and the L2 loops contribute to their stability. Comparison of OmpC porins of the Yersinia pseudotuberculosis complex with other enterobacterial porins demonstrated L2 loop length diversity, which is caused by varying numbers of dipeptide/tripeptide repeats. The OmpC porins are highly homologous to each other, and they can be subdivided into five isoforms based on their L2 loop structure. Optical spectroscopy and SDS-PAGE experiments revealed that particularities of the L2 loops affected the structure and thermal stability of the porins...
October 13, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28957374/synthesis-and-characterization-of-modified-%C3%AE%C2%BA-carrageenan-for-enhanced-proton-conductivity-as-polymer-electrolyte-membrane
#9
Joy Wei Yi Liew, Kee Shyuan Loh, Azizan Ahmad, Kean Long Lim, Wan Ramli Wan Daud
Polymer electrolyte membranes based on the natural polymer κ-carrageenan were modified and characterized for application in electrochemical devices. In general, pure κ-carrageenan membranes show a low ionic conductivity. New membranes were developed by chemically modifying κ-carrageenan via phosphorylation to produce O-methylene phosphonic κ-carrageenan (OMPC), which showed enhanced membrane conductivity. The membranes were prepared by a solution casting method. The chemical structure of OMPC samples were characterized using Fourier transform infrared spectroscopy (FTIR), 1H nuclear magnetic resonance (1H NMR) spectroscopy and 31P nuclear magnetic resonance (31P NMR) spectroscopy...
2017: PloS One
https://www.readbyqxmd.com/read/28940167/th2-biased-immune-response-and-agglutinating-antibodies-generation-by-a-chimeric-protein-comprising-ompc-epitope-323-336-of-aeromonas-hydrophila-and-ltb
#10
Mahima Sharma, Pujarini Dash, Pramod K Sahoo, Aparna Dixit
Aeromonas hydrophila is responsible for causing fatal infections in freshwater fishes. Besides chemical/antibiotic treatment and whole-cell vaccine, no subunit vaccine is currently available for A. hydrophila. Outer membrane proteins of gram-negative bacteria have been reported as effective vaccine candidates. Peptide antigens elicit focused immune responses against immunodominant stretches of the antigen. We have attempted to characterize the immunogenicity of linear B-cell epitopes of outer membrane protein (OmpC) of A...
September 22, 2017: Immunologic Research
https://www.readbyqxmd.com/read/28890516/molecular-characterization-of-non-carbapenemase-producing-escherichia-coli-clinical-isolates-with-reduced-carbapenem-susceptibility-from-a-thai-university-hospital
#11
Sawitree Nuramrum, Aroonwadee Chanawong, Kamonwan Lunha, Aroonlug Lulitanond, Arunnee Sangka, Chotechana Wilailuckana, Sunpetch Angkititrakul, Nicha Charoensri, Lumyai Wonglakorn, Prajuab Chaimanee, Ploenchan Chetchotisakd
Twelve non-replicate carbapenemase-negative ertapenem-non-susceptible (CNENS) Escherichia coli isolates from a Thai university hospital between 2010 and 2014 were characterized and compared with two carbapenemase-producing E. coli isolates from the same hospital. Eight unique PFGE patterns were obtained. All produced CTX-M-15 β-lactamase and two isolates co-expressed either CMY-2 cephalosporinase or increased efflux pump. Amino acid sequence analysis revealed that OmpF defect (seven isolates) due to mutations generating premature proteins or an IS1 insertion was more prevalent than in OmpC (no truncated proteins found)...
September 11, 2017: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28887208/proteomic-comparison-of-three-clinical-diarrhoeagenic-drug-resistant-escherichia-coli-isolates-grown-on-chromagar%C3%A2-stec-media
#12
John Bosco Kalule, Suereta Fortuin, Bridget Calder, Lourens Robberts, Karen H Keddy, Andrew Nel, Shaun Garnett, Mark Nicol, Digby F Warner, Nelson C Soares, Jonathan M Blackburn
Shiga-toxin-producing Escherichia coli (STEC) and enteropathogenic Escherichia coli (EPEC) are key diarrhoea-causing foodborne pathogens. We used proteomics to characterize the virulence and antimicrobial resistance protein profiles of three clinical pathogenic E. coli isolates (two EPEC [one resistant to ciprofloxacin] and one STEC) cultured on CHROMagar™STEC solid media after minimal laboratory passage. We identified 4767 unique peptides from 1630 protein group across all three clinical E. coli strains...
September 5, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28873442/use-of-a-novel-antigen-expressing-system-to-study-the-salmonella-enterica-serovar-typhi-protein-recognition-by-t-cells
#13
Rosângela Salerno-Gonçalves, Hervé Tettelin, David Lou, Stephanie Steiner, Tasmia Rezwanul, Qin Guo, William D Picking, Vishvanath Nene, Marcelo B Sztein
Salmonella enterica serovar Typhi (S. Typhi), the causative agent of the typhoid fever, is a pathogen of great public health importance. Typhoid vaccines have the potential to be cost-effective measures towards combating this disease, yet the antigens triggering host protective immune responses are largely unknown. Given the key role of cellular-mediated immunity in S. Typhi protection, it is crucial to identify S. Typhi proteins involved in T-cell responses. Here, cells from individuals immunized with Ty21a typhoid vaccine were collected before and after immunization and used as effectors...
September 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28816443/characterization-of-ciprofloxacin-permeation-pathways-across-the-porin-ompc-using-metadynamics-and-a-string-method
#14
Jigneshkumar Dahyabhai Prajapati, Carlos José Fernández Solano, Mathias Winterhalter, Ulrich Kleinekathöfer
The rapid spreading of antimicrobial resistance in Gram-negative bacteria has become a major threat for humans as well as animals. As one of the main factors involved, the permeability of the outer membrane has attracted a great deal of attention recently. However, the knowledge regarding the translocation mechanisms for most available antibiotics is so far rather limited. Here, a theoretical study concerning the diffusion route of ciprofloxacin across the outer membrane porin OmpC from E. coli is presented...
August 30, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/28721849/effective-protection-of-mice-against-shigella-flexneri-with-a-new-self-adjuvant-multicomponent-vaccine
#15
Yadira Pastor, Ana Camacho, Ana Gloria Gil, Rocío Ramos, Adela López de Ceráin, Iván Peñuelas, Juan M Irache, Carlos Gamazo
PURPOSE: The aim of this study was to develop an immunogenic protective product against Shigella flexneri by employing a simple and safe heat treatment-based strategy. METHODOLOGY: The physicochemical characteristics of naturally produced (OMV) and heat-induced (HT) outer-membrane vesicles from S. flexneri were examined, including a comparison of the protein content of the products. Toxicological and biodistribution studies, and a preliminary experiment to examine the protective effectiveness of HT in a murine model of S...
July 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28666873/overexpression-of-mica-induces-production-of-ompc-enriched-outer-membrane-vesicles-that-protect-against-salmonella-challenge
#16
Hyun-Il Choi, Moonjeong Kim, Jinseong Jeon, Jin Kwan Han, Kwang-Sun Kim
Outer membrane vesicles (OMVs) derived from bacteria are promising candidates for subunit vaccines. Stresses that modulate the composition of outer membrane proteins (OMPs) are important for OMV synthesis. Small RNAs (sRNAs) expressed in response to stress regulate OMPs, although the mechanism underlying sRNA-mediated OMV biogenesis and its utility for developing vaccine platforms remains to be elucidated. Here, we characterized the role of a sRNA, MicA, which regulates OmpA, a major OMP involved in both production of OMVs and reactive immunity against Salmonella challenge...
August 26, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28650688/multidrug-resistant-enterobacteriaceae-in-lebanese-hospital-wastewater-implication-in-the-one-health-concept
#17
Ziad Daoud, Jina Farah, Elie Salem Sokhn, Khalil El Kfoury, Elias Dahdouh, Khalil Masri, Claude Afif, Roula M Abdel-Massih, Ghassan M Matar
Extended-spectrum beta-lactamases (ESBL) and carbapenemase-producing organisms pose severe problems for hospitalized patients. In the absence of efficient sanitation and sewage disposal, the risks for transmission of hospital organisms into the community are high. Our objectives were to study the occurrence and mechanisms of resistance of multidrug-resistant gram-negative bacilli in two Lebanese hospital sewage treatment plants. Wastewater samples were collected, filtered, and cultivated on MacConckey+cefotaxime agars...
June 26, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28629229/colistin-resistance-associated-with-outer-membrane-protein-change-in%C3%A2-klebsiella-pneumoniae-and-enterobacter-asburiae
#18
Béla Kádár, Béla Kocsis, Ákos Tóth, Katalin Kristóf, Péter Felső, Béla Kocsis, Katalin Böddi, Dóra Szabó
In this study, outer membrane proteins (OMPs) of colistin-resistant Klebsiella pneumoniae and Enterobacter asburiae were analyzed. One colistin-susceptible and three colistin-resistant K. pneumoniae sequence type 258 strains as well as one colistin-susceptible E. asburiae and its colistin-heteroresistant counterpart strain were involved in the study. OMP analysis of each strain was performed by microchip method. Matrix-assisted laser desorption ionization time of flight/mass spectrometry (MALDI-TOF/MS) investigation was carried out after separation of OMPs by two-dimensional gel electrophoresis and in-gel digestion...
June 1, 2017: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/28570603/evolution-of-salmonella-typhi-outer-membrane-protein-specific-t-and-b-cell-responses-in-humans-following-oral-ty21a-vaccination-a-randomized-clinical-trial
#19
RANDOMIZED CONTROLLED TRIAL
Juan Manuel Carreño, Christian Perez-Shibayama, Cristina Gil-Cruz, Constantino Lopez-Macias, Pietro Vernazza, Burkhard Ludewig, Werner C Albrich
Vaccination against complex pathogens such as typhoidal and non-typhoidal Salmonella requires the concerted action of different immune effector mechanisms. Outer membrane proteins (Omps) of Salmonella Typhi are potent immunogens, which elicit long-lasting and protective immunity. Here, we followed the evolution of S. Typhi OmpC and F-specific T and B cell responses in healthy volunteers after vaccination with the vaccine strain Ty21a. To follow humoral and cellular immune responses, pre- and post-vaccination samples (PBMC, serum and stool) collected from 15 vaccinated and 5 non-vaccinated individuals...
2017: PloS One
https://www.readbyqxmd.com/read/28413638/serologic-testing-of-a-panel-of-five-antibodies-in-inflammatory-bowel-diseases-diagnostic-value-and-correlation-with-disease-phenotype
#20
Zhi-Zhi Wang, Ke Shi, Jie Peng
The aim of the present study was to evaluate the diagnostic value of five serological antibodies, perinuclear antineutrophil cytoplasmic antibody (pANCA), anti-Saccharomyces cerevisiae antibodies [ASCA; ASCA-immunoglobulin (IgG)and ASCA-IgA], Escherichia coli outer membrane porin C antibody (anti-OmpC) and CBir1 flagellin antibody for detection in inflammatory bowel diseases. Whether the antibody status correlated with the disease phenotype was also evaluated. Sera from 71 patients with Crohn's disease (CD), 41 patients with ulcerative colitis (UC), 78 patients with other gastrointestinal diseases and 31 healthy control subjects were investigated...
April 2017: Biomedical Reports
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