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https://www.readbyqxmd.com/read/29718391/serologic-reactivity-reflects-clinical-expression-of-ulcerative-colitis-in-children
#1
Elizabeth A Spencer, Sonia M Davis, David R Mack, Brendan M Boyle, Anne M Griffiths, Neal S LeLeiko, Cary G Sauer, David J Keljo, James F Markowitz, Susan S Baker, Joel R Rosh, Robert N Baldassano, Maria Oliva-Hemker, Marian D Pfefferkorn, Anthony R Otley, Melvin B Heyman, Joshua D Noe, Ashish S Patel, Paul A Rufo, M Alison Marquis, Thomas D Walters, Margaret H Collins, Subra Kugathasan, Lee A Denson, Jeffrey S Hyams, Marla C Dubinsky
Background: In contrast to pediatric Crohn's disease (CD), little is known in pediatric ulcerative colitis (UC) about the relationship between disease phenotype and serologic reactivity to microbial and other antigens. Aim: The aim of this study was to examine disease phenotype and serology in a well-characterized inception cohort of children newly diagnosed with UC during the PROTECT Study (Predicting Response to Standardized Pediatric Colitis Therapy). Methods: Patients were recruited from 29 participating centers...
April 30, 2018: Inflammatory Bowel Diseases
https://www.readbyqxmd.com/read/29657131/unusual-constriction-zones-in-the-major-porins-ompu-and-ompt-from-vibrio-cholerae
#2
Monisha Pathania, Silvia Acosta-Gutierrez, Satya Pratyusha Bhamidimarri, Arnaud Baslé, Mathias Winterhalter, Matteo Ceccarelli, Bert van den Berg
The outer membranes (OM) of many Gram-negative bacteria contain general porins, which form nonspecific, large-diameter channels for the diffusional uptake of small molecules required for cell growth and function. While the porins of Enterobacteriaceae (e.g., E. coli OmpF and OmpC) have been extensively characterized structurally and biochemically, much less is known about their counterparts in Vibrionaceae. Vibrio cholerae, the causative agent of cholera, has two major porins, OmpU and OmpT, for which no structural information is available despite their importance for the bacterium...
April 3, 2018: Structure
https://www.readbyqxmd.com/read/29611135/a-chimeric-two-component-regulatory-system-based-escherichia-coli-biosensor-engineered-to-detect-glutamate
#3
Sambandam Ravikumar, Yokimiko David, Si Jae Park, Jong-Il Choi
In this study, we constructed amino acid biosensors that can be used as a high-throughput system to screen microorganisms that produce glutamate. The biosensors are based on two-component regulatory systems (TCRSs) combined with green fluorescent protein (GFP) as a reporter. A chimeric DegS/EnvZ (DegSZ) TCRS was constructed by fusing the N-terminal domain of the sensor kinase DegS from Planococcus sp. PAMC21323 with the catalytic domain of the osmosensor EnvZ from Escherichia coli to control expression of gfp in response to glutamate...
April 2, 2018: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/29543429/single-molecule-detection-reveals-different-roles-of-skp-and-sura-as-chaperones
#4
Geng Li, Chenhui He, Peixuan Bu, Huimin Bi, Sichen Pan, Ronghua Sun, Xin Sheng Zhao
Skp and SurA are both periplasmic chaperones involved in the biogenesis of Escherichia coli β-barrel outer membrane proteins (OMPs). It is commonly assumed that SurA plays a major role whereas Skp is a minor factor. However, there is no molecular evidence for whether their roles are redundant. Here, by using different dilution methods, we obtained monodisperse and aggregated forms of OmpC and studied their interactions with Skp and SurA by single-molecule fluorescence resonance energy transfer and fluorescence correlation spectroscopy...
April 20, 2018: ACS Chemical Biology
https://www.readbyqxmd.com/read/29518576/identification-of-ethanol-tolerant-outer-membrane-proteome-reveals-ompc-dependent-mechanism-in-a-manner-of-envz-ompr-regulation-in-escherichia-coli
#5
Dan-Feng Zhang, Jin-Zhou Ye, Hong-Hou Dai, Xiang-Min Lin, Hui Li, Xuan-Xian Peng
Ethanol is an efficient disinfectant, but long-term and wide usage of ethanol leads to microbial tolerance. Bacteria with the tolerance are widely identified. However, mechanisms of the tolerance are not elucidated. To explore the mechanisms of outer membrane (OM) proteins underlying ethanol tolerance in bacteria, functional proteomic methodologies were utilized to characterize OM proteins of E. coli suddenly exposed to 3.125% ethanol. Of eleven proteins altered significantly, seven were OM proteins, in which LamB, FadL and OmpC were up-regulated, and OmpT, OmpF, Tsx and OmpA were down-regulated...
May 15, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29458539/immunogenicity-in-chickens-with-orally-administered-recombinant-chicken-borne-lactobacillus-saerimneri-expressing-fima-and-ompc-antigen-of-o78-avian-pathogenic-escherichia-coli
#6
Sun-Ting Ma, Guo-Jie Ding, Xue-Wei Huang, Zi-Wei Wang, Li Wang, Mei-Ling Yu, Wen Shi, Yan-Ping Jiang, Li-Jie Tang, Yi-Gang Xu, Yi-Jing Li
PURPOSE: Avian colibacillosis is responsible for economic losses to poultry producers worldwide. To combat this, we aimed to develop an effective oral vaccine for chicken against O78 avian pathogenic Escherichia coli (APEC) infection through a Lactobacillus delivery system. METHODOLOGY: Eight Lactobacillus strains isolated from the intestines of broiler chickens were evaluated based on their in vitro adherence ability to assess their potential as a delivery vector...
March 2018: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29399114/mechanism-for-carbapenem-resistance-of-clinical-enterobacteriaceae-isolates
#7
Yafei Ye, Lijuan Xu, Yanping Han, Zhe Chen, Cailin Liu, Liang Ming
Carbapenemase-producing 'super bacteria', particularly NDM-1 and its variants, have become a major public health concern worldwide. The present study aimed to explore the molecular mechanism for carbapenem resistance of clinical Enterobacteriaceae isolates. Seventy-eight non-repeated Enterobacteriaceae strains resistant to any carbapenem were screened at the First Affiliated Hospital of Zhengzhou University (Zhengzhou, China) between December 2011 and December 2015. Outer membrane porin (OMP) proteins were detected using SDS-PAGE...
January 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29370221/surface-display-of-ompc-of-salmonella-serovar-pullorum-on-bacillus-subtilis-spores
#8
Xixi Dai, Minggang Liu, Kangcheng Pan, Jinlong Yang
Salmonellosis is a major public health problem throughout the world. Thus, there is a huge need for diversified control strategies for Salmonella infections. In this work, we have assessed the potential use of Bacillus subtilis (B. subtilis) spores for the expression of a major protective antigen of Salmonella serovar Pullorum, OmpC. The expression of OmpC on the surface of spores was determined by immunofluorescence microscopy. Mice immunized with recombinant spores expressing the OmpC antigen presented significant levels of OmpC-specific serum IgG and mucosal SIgA antibodies than in mice immunized with non-recombinant spores (p<0...
2018: PloS One
https://www.readbyqxmd.com/read/29309834/recombinant-hsp33-and-ompc-protein-can-serve-as-promising-divalent-vaccine-with-protection-against-vibrio-anguillarum-and-edwardsiella-tarda-in-flounder-paralichthys-olivaceus
#9
Jing Xing, Pengwei Li, Xiaoqian Tang, Wenbin Zhan
Vibrio anguillarum and Edwardsiella tarda are severe aquaculture pathogens shared similar epidemiological characteristics and susceptible to flounder (Paralichthys olivaceus). In our previous studies, recombinant(r) protein heat shock protein 33 (rHsp33) from V. anguillarum and outer membrane protein C (rOmpC) from E. tarda were proved to have protection against V. anguillarum and E. tarda, respectively. In this paper, the cross protection of rHsp33 against E. tarda and rOmpC against V. anguillarum, and the protection of divalent vaccine candidate (rHsp33 + rOmpC, rHC) against both V...
March 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29307192/enrofloxacin-permeation-pathways-across-the-porin-ompc
#10
Jigneshkumar Dahyabhai Prajapati, Carlos José Fernández Solano, Mathias Winterhalter, Ulrich Kleinekathöfer
In Gram-negative bacteria, the lack or quenching of antibiotic translocation across the outer membrane is one of the main factors for acquiring antibiotic resistance. An atomic-level comprehension of the key features governing the transport of drugs by outer-membrane protein channels would be very helpful in developing the next generation of antibiotics. In a previous study [ J. D. Prajapati et al. J. Chem. Theory Comput. 2017 , 13 , 4553 ], we characterized the diffusion pathway of a ciprofloxacin molecule through the outer membrane porin OmpC of Escherichia coli by combining metadynamics and a zero-temperature string method...
February 1, 2018: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29211019/dual-regulation-of-the-small-rna-micc-and-the-quiescent-porin-ompn-in-response-to-antibiotic-stress-in-escherichia-coli
#11
Sushovan Dam, Jean-Marie Pagès, Muriel Masi
Antibiotic resistant Gram-negative bacteria are a serious threat for public health. The permeation of antibiotics through their outer membrane is largely dependent on porin, changes in which cause reduced drug uptake and efficacy. Escherichia coli produces two major porins, OmpF and OmpC. MicF and MicC are small non-coding RNAs (sRNAs) that modulate the expression of OmpF and OmpC, respectively. In this work, we investigated factors that lead to increased production of MicC. micC promoter region was fused to lacZ , and the reporter plasmid was transformed into E...
December 6, 2017: Antibiotics
https://www.readbyqxmd.com/read/29185080/manganese-and-cobalt-recovery-by-surface-display-of-metal-binding-peptide-on-various-loops-of-ompc-in-escherichia-coli
#12
Murali Kannan Maruthamuthu, Vidhya Selvamani, Saravanan Prabhu Nadarajan, Hyungdon Yun, You-Kwan Oh, Gyeong Tae Eom, Soon Ho Hong
In a cell-surface display (CSD) system, successful display of a protein or peptide is highly dependent on the anchoring motif and the position of the display in that anchoring motif. In this study, a recombinant bacterial CSD system for manganese (Mn) and cobalt (Co) recovery was developed by employing OmpC as an anchoring motif on three different external loops. A portion of Cap43 protein (TRSRSHTSEG)3 was employed as a manganese and cobalt binding peptide (MCBP), which was fused with OmpC at three different external loops...
January 2018: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/29180369/membrane-vesicles-derived-from-bordetella-bronchiseptica-active-constituent-of-a-new-vaccine-against-infections-caused-by-this-pathogen
#13
D Bottero, M E Zurita, M E Gaillard, E Bartel, C Vercellini, D Hozbor
Bordetella bronchiseptica , a Gram-negative bacterium, causes chronic respiratory tract infections in a wide variety of mammalian hosts, including humans (albeit rarely). We recently designed Bordetella pertussis and Bordetella parapertussis experimental vaccines based on outer membrane vesicles (OMVs) derived from each pathogen, and we obtained protection against the respective infections in mice. Here, we demonstrated that OMVs derived from virulent-phase B. bronchiseptica (OMVBbvir+ ) protected mice against sublethal infections with different B...
February 15, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29164072/a-new-strain-collection-for-improved-expression-of-outer-membrane-proteins
#14
Ina Meuskens, Marcin Michalik, Nandini Chauhan, Dirk Linke, Jack C Leo
Almost all integral membrane proteins found in the outer membranes of Gram-negative bacteria belong to the transmembrane β-barrel family. These proteins are not only important for nutrient uptake and homeostasis, but are also involved in such processes as adhesion, protein secretion, biofilm formation, and virulence. As surface exposed molecules, outer membrane β-barrel proteins are also potential drug and vaccine targets. High production levels of heterologously expressed proteins are desirable for biochemical and especially structural studies, but over-expression and subsequent purification of membrane proteins, including outer membrane proteins, can be challenging...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29055550/the-phylogenetic-group-antimicrobial-susceptibility-and-virulence-genes-of-escherichia-coli-from-clinical-bovine-mastitis
#15
Dexian Zhang, Zehui Zhang, Chengcheng Huang, Xiang Gao, Zhuang Wang, Yaochuan Liu, Chunlian Tian, Wei Hong, Shengli Niu, Mingchun Liu
Bovine mastitis is still a central problem on dairy farms despite control programs, and Escherichia coli is a crucial pathogen during the development of bovine mastitis. The virulence genes, antimicrobial susceptibility, and mortality of mice infected with different E. coli isolates from bovine mastitis were determined in this study. According to the presence of the specific genes chuA, yjaA, and TspE4.C2, these isolates mainly belonged to 2 different groups: group A (47/79) and group B1 (22/79). The ompC gene was detected in all the isolates, followed by fimH (89...
January 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29038023/the-impact-of-length-variations-in-the-l2-loop-on-the-structure-and-thermal-stability-of-non-specific-porins-the-case-of-ompcs-from-the-yersinia-pseudotuberculosis-complex
#16
T Solov'eva, G Likhatskaya, V Khomenko, K Guzev, N Kim, E Bystritskaya, O Novikova, A Stenkova, A Rakin, M Isaeva
Porins are integral proteins of the outer membranes of gram-negative bacteria. In membranes, they exist as homotrimers and the L2 loops contribute to their stability. Comparison of OmpC porins of the Yersinia pseudotuberculosis complex with other enterobacterial porins demonstrated L2 loop length diversity, which is caused by varying numbers of dipeptide/tripeptide repeats. The OmpC porins are highly homologous to each other, and they can be subdivided into five isoforms based on their L2 loop structure. Optical spectroscopy and SDS-PAGE experiments revealed that particularities of the L2 loops affected the structure and thermal stability of the porins...
February 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28957374/synthesis-and-characterization-of-modified-%C3%AE%C2%BA-carrageenan-for-enhanced-proton-conductivity-as-polymer-electrolyte-membrane
#17
Joy Wei Yi Liew, Kee Shyuan Loh, Azizan Ahmad, Kean Long Lim, Wan Ramli Wan Daud
Polymer electrolyte membranes based on the natural polymer κ-carrageenan were modified and characterized for application in electrochemical devices. In general, pure κ-carrageenan membranes show a low ionic conductivity. New membranes were developed by chemically modifying κ-carrageenan via phosphorylation to produce O-methylene phosphonic κ-carrageenan (OMPC), which showed enhanced membrane conductivity. The membranes were prepared by a solution casting method. The chemical structure of OMPC samples were characterized using Fourier transform infrared spectroscopy (FTIR), 1H nuclear magnetic resonance (1H NMR) spectroscopy and 31P nuclear magnetic resonance (31P NMR) spectroscopy...
2017: PloS One
https://www.readbyqxmd.com/read/28940167/th2-biased-immune-response-and-agglutinating-antibodies-generation-by-a-chimeric-protein-comprising-ompc-epitope-323-336-of-aeromonas-hydrophila-and-ltb
#18
Mahima Sharma, Pujarini Dash, Pramod K Sahoo, Aparna Dixit
Aeromonas hydrophila is responsible for causing fatal infections in freshwater fishes. Besides chemical/antibiotic treatment and whole-cell vaccine, no subunit vaccine is currently available for A. hydrophila. Outer membrane proteins of gram-negative bacteria have been reported as effective vaccine candidates. Peptide antigens elicit focused immune responses against immunodominant stretches of the antigen. We have attempted to characterize the immunogenicity of linear B-cell epitopes of outer membrane protein (OmpC) of A...
February 2018: Immunologic Research
https://www.readbyqxmd.com/read/28890516/molecular-characterization-of-non-carbapenemase-producing-escherichia-coli-clinical-isolates-with-reduced-carbapenem-susceptibility-from-a-thai-university-hospital
#19
Sawitree Nuramrum, Aroonwadee Chanawong, Kamonwan Lunha, Aroonlug Lulitanond, Arunnee Sangka, Chotechana Wilailuckana, Sunpetch Angkititrakul, Nicha Charoensri, Lumyai Wonglakorn, Prajuab Chaimanee, Ploenchan Chetchotisakd
Twelve non-replicate carbapenemase-negative ertapenem-non-susceptible (CNENS) Escherichia coli isolates from a Thai university hospital between 2010 and 2014 were characterized and compared with two carbapenemase-producing E. coli isolates from the same hospital. Eight unique PFGE patterns were obtained. All produced CTX-M-15 β-lactamase and two isolates co-expressed either CMY-2 cephalosporinase or increased efflux pump. Amino acid sequence analysis revealed that OmpF defect (seven isolates) due to mutations generating premature proteins or an IS1 insertion was more prevalent than in OmpC (no truncated proteins found)...
September 11, 2017: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28887208/proteomic-comparison-of-three-clinical-diarrhoeagenic-drug-resistant-escherichia-coli-isolates-grown-on-chromagar%C3%A2-stec-media
#20
John Bosco Kalule, Suereta Fortuin, Bridget Calder, Lourens Robberts, Karen H Keddy, Andrew Nel, Shaun Garnett, Mark Nicol, Digby F Warner, Nelson C Soares, Jonathan M Blackburn
Shiga-toxin-producing Escherichia coli (STEC) and enteropathogenic Escherichia coli (EPEC) are key diarrhoea-causing foodborne pathogens. We used proteomics to characterize the virulence and antimicrobial resistance protein profiles of three clinical pathogenic E. coli isolates (two EPEC [one resistant to ciprofloxacin] and one STEC) cultured on CHROMagar™STEC solid media after minimal laboratory passage. We identified 4767 unique peptides from 1630 protein group across all three clinical E. coli strains...
September 5, 2017: Journal of Proteomics
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