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https://www.readbyqxmd.com/read/29452198/microbial-infection-pattern-pathogenic-features-and-resistance-mechanism-of-carbapenem-resistant-gram-negative-bacilli-during-long-term-hospitalization
#1
Shuxian Wen, Donghua Feng, Zerong Lu, Junyan Liu, Brian M Peters, Hailing Tang, Danhong Su, Yong-Ping Lin, Ling Yang, Zhenbo Xu, Mark E Shirtliff, Dingqiang Chen
BACKGROUND: Carbapenem-resistant Gram-negative bacilli (GNB) have become an important cause of nosocomial infections of hospitalized patients. METHODS: To investigate the microbial infection patterns and molecular epidemiology characteristics of the carbapenem-resistant GNB isolates from a long-term hospitalized patient, antimicrobial susceptibility testing, phenotypic screening test for carbapenemase production, PCR screening and DNA sequencing of carbapenemase genes, repetitive extragenic palindromic sequence-based PCR (REP-PCR), multilocus sequencing typing (MLST) and genetic environment analysis were performed...
February 13, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29431605/multifactorial-chromosomal-variants-regulate-polymyxin-resistance-in-extensively-drug-resistant-klebsiella-pneumoniae
#2
Miranda E Pitt, Alysha G Elliott, Minh Duc Cao, Devika Ganesamoorthy, Ilias Karaiskos, Helen Giamarellou, Cely S Abboud, Mark A T Blaskovich, Matthew A Cooper, Lachlan J M Coin
Extensively drug-resistant Klebsiella pneumoniae (XDR-KP) infections cause high mortality and are disseminating globally. Identifying the genetic basis underpinning resistance allows for rapid diagnosis and treatment. XDR isolates sourced from Greece and Brazil, including 19 polymyxin-resistant and five polymyxin-susceptible strains, were subjected to whole genome sequencing. Seventeen of the 19 polymyxin-resistant isolates harboured variations upstream or within mgrB. The most common mutation identified was an insertion at nucleotide position 75 in mgrB via an ISKpn26-like element in the ST258 lineage and ISKpn13 in one ST11 isolate...
February 12, 2018: Microbial Genomics
https://www.readbyqxmd.com/read/29430190/salmonella-enterica-serovars-typhimurium-and-enteritidis-causing-mixed-infections-in-febrile-children-in-mozambique
#3
Vanesa García, Inácio Mandomando, Joaquim Ruiz, Silvia Herrera-León, Pedro L Alonso, M Rosario Rodicio
Background and purpose: Invasive nontyphoidal salmonellosis, mostly caused by serovars Typhimurium and Enteritidis of Salmonella enterica, has emerged as a major public health problem in sub-Saharan Africa. The aim of this study was the clinical and microbiological characterization of nontyphoidal salmonellosis episodes affecting febrile children in Mozambique. Patients and methods: The clinical records of the patients were evaluated, and S. enterica isolates were characterized with regard to serovar, phage type, antimicrobial resistance (phenotype/responsible genes), plasmid content, pulsed-field gel electrophoresis, and multilocus sequence typing...
2018: Infection and Drug Resistance
https://www.readbyqxmd.com/read/29424684/genome-analysis-of-clinical-multilocus-sequence-type-11-klebsiella-pneumoniae-from-china
#4
Ning Dong, Rong Zhang, Lizhang Liu, Ruichao Li, Dachuan Lin, Edward Wai-Chi Chan, Sheng Chen
The increasing prevalence of KPC-producing Klebsiella pneumoniae strains in clinical settings has been largely attributed to dissemination of organisms of specific multilocus sequence types, such as ST258 and ST11. Compared with the ST258 clone, which is prevalent in North America and Europe, ST11 is common in China but information regarding its genetic features remains scarce. In this study, we performed detailed genetic characterization of ST11 K. pneumoniae strains by analyzing whole-genome sequences of 58 clinical strains collected from diverse geographic locations in China...
February 8, 2018: Microbial Genomics
https://www.readbyqxmd.com/read/29422888/klebsiella-pneumoniae-carbapenemase-producers-in-south-korea-between-2013-and-2015
#5
Eun-Jeong Yoon, Jung Ok Kim, Dokyun Kim, Hyukmin Lee, Ji Woo Yang, Kwang Jun Lee, Seok Hoon Jeong
Between 2014 and 2015, the Klebsiella pneumoniae carbapenemase (KPC) was becoming endemic in South Korea. To assess this period of transition, we analyzed KPC producers in terms of molecular epidemiology. A total of 362 KPC-producing Enterobacteriaceae strains, including one from 2013, 13 from 2014, and 348 from 2015, were actively collected from 60 hospitals throughout the peninsula. Subtypes of KPC were determined by PCR and direct sequencing, and isotypes of Tn4401 (the transposon flanking the blaKPC gene) were specified by PCR using isotype-specific primers and direct sequencing...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29402927/salmonella-enteritidis-st183-emerging-and-endemic-biotypes-affecting-western-european-hedgehogs-erinaceus-europaeus-and-people-in-great-britain
#6
Becki Lawson, Lydia H V Franklinos, Julia Rodriguez-Ramos Fernandez, Clare Wend-Hansen, Satheesh Nair, Shaheed K Macgregor, Shinto K John, Romain Pizzi, Alejandro Núñez, Philip M Ashton, Andrew A Cunningham, Elizabeth M de Pinna
The impacts of hedgehog (Erinaceus europaeus) Salmonella infection on public health and on animal welfare and conservation are unknown. We isolated Salmonella Enteritidis multi-locus sequence-type (ST)183 from 46/170 (27%) hedgehog carcasses (27 S. Enteritidis phage type (PT)11, 18 of a novel PT66 biotype and one with co-infection of these PTs) and from 6/208 (3%) hedgehog faecal samples (4 PT11, 2 PT66) from across Great Britain, 2012-2015. Whole genome phylogenetic analysis of the hedgehog isolates and ST183 from people in England and Wales found that PT11 and PT66 form two divergent clades...
February 5, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29360567/frequency-of-virulence-factors-in-high-biofilm-formation-blakpc-2-producing-klebsiella-pneumoniae-strains-from-hospitals
#7
Li Fu, Mei Huang, XinZhou Zhang, XiaoYing Yang, Yao Liu, LuHua Zhang, ZhiKun Zhang, GuangXi Wang, YingShun Zhou
The aim of this study is to determine the frequency of virulence genes in high biofilm formation blaKPC-2 producing Klebsiella pneumoniae strains collected over a period of two years. A total of 43 non-repetitive high biofilm blaKPC-2 producing isolates were screened from 429 strains. The MIC of carbapenems (imipenem and meropenem) ranged from 4 to 32 μg/ml. The OD595 value of the biofilm ranged from 0.56 to 2.56. The K1, K2, K5, K20, K54, K57 genotypes, MLST and virulence factors, including entB, ybtS, mrkD, fimH, rmpA, allS, iutA, kfu, wcaG, aerobaction, fecIRA, shiF, magA and pagO gene, were determined by PCR...
January 19, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29329299/multi-drug-resistant-non-typhoidal-salmonella-associated-with-invasive-disease-in-western-kenya
#8
Adam Akullian, Joel M Montgomery, Grace John-Stewart, Samuel I Miller, Hillary S Hayden, Matthew C Radey, Kyle R Hager, Jennifer R Verani, John Benjamin Ochieng, Jane Juma, Jim Katieno, Barry Fields, Godfrey Bigogo, Allan Audi, Judd Walson
Non-typhoidal Salmonella (NTS) is a leading cause of bloodstream infections in Africa, but the various contributions of host susceptibility versus unique pathogen virulence factors are unclear. We used data from a population-based surveillance platform (population ~25,000) between 2007-2014 and NTS genome-sequencing to compare host and pathogen-specific factors between individuals presenting with NTS bacteremia and those presenting with NTS diarrhea. Salmonella Typhimurium ST313 and Salmonella Enteritidis ST11 were the most common isolates...
January 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29319505/kpc-2-producing-klebsiella-pneumoniae-st11-in-a-children-s-hospital-in-poland
#9
Monika Machulska, Anna Baraniak, Iwona Żak, Katarzyna Bojarska, Dorota Żabicka, Iwona Sowa-Sierant, Waleria Hryniewicz, Marek Gniadkowski
Four Klebsiella pneumoniae isolates from children hospitalized over 10 months in an intensive care unit in a children's teaching hospital in Poland were analyzed. All of the isolates belonged to a single pulsotype and sequence type (ST) 11, and produced the KPC-2 carbapenemase and extended-spectrum β-lactamase (ESBL) CTX-M-15. They were resistant to a variety of antimicrobials, and their β-lactam resistance patterns were typical for KPC producers. This is one of few cases of identification of KPC (or carbapenemase)-producing K...
September 27, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/29303020/risk-factors-outcomes-and-genotypes-of-carbapenem-nonsusceptible-klebsiella-pneumoniae-bloodstream-infection-a-three-year-retrospective-study-in-a-large-tertiary-hospital-in-northern-china
#10
Si-Han Zheng, Sheng-Ji Cao, Hui Xu, Dan Feng, Li-Ping Wan, Guang-Jie Wang, Xiao-Guang Xiao
OBJECTIVE: To investigate the independent risk factors, outcomes and genotypes associated with carbapenem-non-susceptible K. pneumoniae bloodstream infections (BSIs) in northern China from 2014 to 2016. METHODS: Over a three-year period, a total of 289 K. pneumoniae BSI patients were identified. Medical records were extracted to obtain the clinical information. Polymerase chain reactions (PCRs) were performed to analyse the multilocus sequence typing (MLST) types, Klebsiella pneumoniae carbapenemase (KPC) and metallo-β-lactamases (MBL) genes, for replicon typing of the 10 randomly selected carbapenem-non-susceptible K...
January 5, 2018: Infectious Diseases
https://www.readbyqxmd.com/read/29282569/emergence-and-establishment-of-kpc-2-producing-st11-klebsiella-pneumoniae-in-a-general-hospital-in-shanghai-china
#11
Jingxian Liu, Jing Yu, Feng Chen, Jiajia Yu, Patricia Simner, Pranita Tamma, Ying Liu, Lisong Shen
The aim of this study was to investigate the characteristics of carbapenem-resistant Klebsiella pneumoniae (CRKP) collected during an outbreak in a Chinese teaching hospital and to provide insights into the prevention and control of nosocomial infection. We collected unique CRKP clinical isolates from 2009 to 2013. Antibiotic-resistant genes were identified by polymerase chain reaction (PCR) and sequencing. The isolates were typed using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST)...
December 27, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29238932/risk-factors-and-clinical-outcomes-of-hypervirulent-klebsiella-pneumoniae-induced-bloodstream-infections
#12
Jiayang Li, Jianan Ren, Weiping Wang, Gefei Wang, Guosheng Gu, Xiuwen Wu, Ying Wang, Mei Huang, Jieshou Li
The prevalence of hypervirulent Klebsiella pneumoniae (hvKP) is high in China, but clinical characteristics and outcomes of hvKP induced bloodstream infections (BSIs) are not clear. The purpose of the present study was to determine the risk factors and clinical outcomes of hvKP-BSIs in populations admitted in a teaching hospital of Nanjing, China. The genetic characteristics and antibiotic resistance patterns of the hvKP strains were further analyzed. A retrospective study was conducted in 143 patients with K...
December 14, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29237086/enterobacteriaceae-producing-oxa-48-like-carbapenemases-in-poland-2013-january-2017
#13
R Izdebski, A Baraniak, D Zabicka, M Machulska, P Urbanowicz, J Fiett, E Literacka, K Bojarska, A Kozinska, B Zieniuk, W Hryniewicz, M Gniadkowski
Objectives: To analyse OXA-48 (OXA-48/181)-type carbapenemase-producing Enterobacteriaceae reported in Poland from 2013 until January 2017. Methods: Bacterial isolates were typed by PFGE and MLST. Genes coding for OXA-48/181 types and other β-lactamases were amplified and sequenced. Mobile elements with blaOXA-48/181-like genes were PCR mapped. blaOXA-48/181-carrying plasmids were characterized by nuclease S1-hybridization profiling, transfer assays and PCR-based replicon typing, while the chromosomal location of the genes was confirmed by the I-CeuI analysis...
December 9, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29232167/tigecycline-susceptibility-of-klebsiella-pneumoniae-complex-and-escherichia-coli-isolates-from-companion-animals-the-prevalence-of-tigecycline-nonsusceptible-k-pneumoniae-complex-including-internationally-expanding-human-pathogenic-lineages
#14
Toyotaka Sato, Kazuki Harada, Masaru Usui, Yuzo Tsuyuki, Tsukasa Shiraishi, Yutaka Tamura, Shin-Ichi Yokota
Transmission of tigecycline-nonsusceptible pathogenic Enterobacteriaceae from companion animals to human should be a concern because tigecycline is a last-line drug for treating multidrug-resistant Enterobacteriaceae in human medicine. However, tigecycline susceptibility of Enterobacteriaceae isolated from companion animals has not been investigated. In this study, we investigated the tigecycline susceptibility of Klebsiella pneumoniae complex and Escherichia coli isolates from dogs and cats, and evaluated their human pathogenicity potential...
December 12, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29229634/genomic-insights-into-colistin-resistant-klebsiella-pneumoniae-from-a-tunisian-teaching-hospital
#15
Nadia Jaidane, Rémy A Bonnin, Wejdene Mansour, Delphine Girlich, Elodie Creton, Garance Colleton, Cherifa Chaouch, Noureddine Boujaafar, Olfa Bouallegue, Thierry Naas
The emergence of colistin-resistant Klebsiella pneumoniae (CoRKp) is a public health concern since this antibiotic has become the last line of treatment for infections caused by multidrug-resistant (MDR) gram-negatives. In this study, we have investigated the molecular basis of colistin-resistance in 13 MDR Kp isolated from 12 patients in a teaching hospital in Sousse, Tunisia. Whole-genome sequencing (WGS) was used to decipher the molecular mechanism of colistin-resistance and to identify the resistome of these CoRKp It revealed a genome of ca...
December 11, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29226698/in-vitro-and-in-vivo-activity-of-manuka-honey-against-ndm-1-producing-klebsiella-pneumoniae-st11
#16
Muhammad Usman Qamar, Sidrah Saleem, Mark Alexander Toleman, Muhammad Saqalein, Muhammad Waseem, Muhammad Atif Nisar, Mohsin Khurshid, Zeeshan Taj, Shah Jahan
AIM: To determine the therapeutic potential of Manuka honey against New Delhi metallo-β-lactamase-1-producing Klebsiella pneumoniae ST11 in vitro and in vivo. MATERIALS & METHODS: Carbapenamases and metallo-β-lactamases-producing K. pneumoniae ST11 isolated from blood culture was confirmed by VITEK-2® system, matrix-assisted laser desorption ionization-time of flight and multilocus sequence typing, followed by determination of minimum inhibitory concentration (μg/ml) using VITEK-2 system...
January 2018: Future Microbiology
https://www.readbyqxmd.com/read/29068275/investigation-of-next-generation-sequencing-data-of-klebsiella-pneumoniae-using-web-based-tools
#17
Eva Brhelova, Mariya Antonova, Filip Pardy, Iva Kocmanova, Jiri Mayer, Zdenek Racil, Martina Lengerova
PURPOSE: Rapid identification and characterization of multidrug-resistant Klebsiella pneumoniae strains is necessary due to the increasing frequency of severe infections in patients. The decreasing cost of next-generation sequencing enables us to obtain a comprehensive overview of genetic information in one step. The aim of this study is to demonstrate and evaluate the utility and scope of the application of web-based databases to next-generation sequenced (NGS) data. METHODOLOGY: The whole genomes of 11 clinical Klebsiella pneumoniae isolates were sequenced using Illumina MiSeq...
October 25, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29040585/emergence-of-mcr-1-and-carbapenemase-genes-in-hospital-sewage-water-in-beijing-china
#18
Longyang Jin, Ruobing Wang, Xiaojuan Wang, Qi Wang, Yawei Zhang, Yuyao Yin, Hui Wang
Objectives: This study identified and characterized mcr-1-positive Enterobacteriaceae (MCRPE) and carbapenemase-producing Enterobacteriaceae (CPE) in hospital sewage water. Methods: Influent and effluent sewage samples were collected from five tertiary hospitals in Beijing in December 2016. Samples were screened for MCRPE and CPE using antibiotic selection media. Results were confirmed by PCR amplification of β-lactamase and colistin resistance (mcr-1 and mcr-2) genes and by sequencing...
October 10, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29036991/-molecular-epidemiology-of-carbapenem-resistant-klebsiella-pneumoniae-in-a-hospital-in-beijing
#19
D K Chen, H J Zhou
Objective: To reveal the molecular epidemiological characteristics of Carbapenem-Resistant Klebsiella pneumoniae (CRKP) isolated from a three level teaching hospital in Beijing. Methods: Pulsed field gel electrophoresis (PFGE) was carried out to subtyping 375 CRKP isolated in that hospital between May 2010 and October 2015. Fifteen strains were chose based on the PFGE patterns to be analyzed by multi-locus sequence typing (MLST) and detection of carbapenem-resistance genes. One strain (A1502) was selected for whole genome sequencing and analyzing...
October 6, 2017: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
https://www.readbyqxmd.com/read/29032055/microbiological-characterization-of-klebsiella-pneumoniae-isolates-causing-bloodstream-infection-from-five-tertiary-hospitals-in-beijing-china
#20
Yanning Ma, Chunmei Bao, Jie Liu, Xiuhong Hao, Jingui Cao, Liyan Ye, Jiyong Yang
BACKGROUND: Klebsiella pneumoniae exhibits prevalence in China. Little is known about the microbiological characterization of clinical K. pneumoniae isolates causing bloodstream infections (BSIs). METHODS: BSI-causing K. pneumoniae (BSIKpn) isolates were collected from five tertiary-care hospitals in Beijing, China. Their genetic relatedness was analyzed by pulsed-field gel electrophoresis (PFGE), antimicrobial susceptibility was determined by agar dilution method, and sequence type (ST) was evaluated by multilocus sequence typing (MLST)...
October 11, 2017: Journal of Global Antimicrobial Resistance
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