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https://www.readbyqxmd.com/read/27909565/non-invasive-methods-for-embryo-selection
#1
REVIEW
H N Sallam, N H Sallam, S H Sallam
With the widespread use of assisted reproduction, a simple and practical method for embryo selection is needed to optimize the chances of pregnancy while diminishing the incidence of multiple pregnancy and its accompanying problems. Many non-invasive methods for embryo selection have been proposed and some are more promising than others. This review summarizes these methods and attempts to evaluate them in the light of the best currently available evidence and to find out whether any of them is ripe for replacing or supplementing the time-honored method of morphological assessment...
June 27, 2016: Facts, Views & Vision in ObGyn
https://www.readbyqxmd.com/read/27908283/expression-of-a-fungal-manganese-peroxidase-in-escherichia-coli-a-comparison-between-the-soluble-and-refolded-enzymes
#2
Nan Wang, Kai Ren, Rong Jia, Wenting Chen, Ruirui Sun
BACKGROUND: Manganese peroxidase (MnP) from Irpex lacteus F17 has been shown to have a strong ability to degrade recalcitrant aromatic pollutants. In this study, a recombinant MnP from I. lacteus F17 was expressed in Escherichia coli Rosetta (DE3) in the form of inclusion bodies, which were refolded to achieve an active enzyme. Further, we optimized the in vitro refolding conditions to increase the recovery yield of the recombinant protein production. Additionally, we attempted to express recombinant MnP in soluble form in E...
December 1, 2016: BMC Biotechnology
https://www.readbyqxmd.com/read/27901038/burden-assessment-in-caregivers-of-patients-with-home-artificial-nutrition-a-need-and-a-challenge
#3
R Villar-Taibo, M A Martínez-Olmos, D Bellido-Guerrero, A Calleja-Fernández, R Peinó-García, A Martís-Sueiro, E Camarero-González, V Ríos-Barreiro, P Cao-Sánchez, R Durán-Martínez, M-J Rodríguez-Iglesias, B Rodríguez-Blanco, J Rojo-Valdés
BACKGROUND/OBJECTIVES: Caregiving can be a stressful task with severe consequences on caregivers' health. Our aim was to evaluate the profile and the burden of caregivers of patients with home artificial nutrition (HAN) in our area. SUBJECTS/METHODS: We conducted a prospective observational study of patients who had started HAN over a period of a year (n=573) and their home caregivers (n=103). Epidemiological characteristics of the patients and the type of HAN were registered...
November 30, 2016: European Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/27898049/mecs-vine-%C3%A2-a-new-proximal-sensor-for-segmented-mapping-of-vigor-and-yield-parameters-on-vineyard-rows
#4
Matteo Gatti, Paolo Dosso, Marco Maurino, Maria Clara Merli, Fabio Bernizzoni, Facundo José Pirez, Bonfiglio Platè, Gian Carlo Bertuzzi, Stefano Poni
Ground-based proximal sensing of vineyard features is gaining interest due to its ability to serve in even quite small plots with the advantage of being conducted concurrently with normal vineyard practices (i.e., spraying, pruning or soil tilling) with no dependence upon weather conditions, external services or law-imposed limitations. The purpose of the present work was to test performance of the new terrestrial multi-sensor MECS-VINE(®) in terms of reliability and degree of correlation with several canopy growth and yield parameters in the grapevine...
November 27, 2016: Sensors
https://www.readbyqxmd.com/read/27897220/impact-of-the-microbial-derived-short-chain-fatty-acid-propionate-on-host-susceptibility-to-bacterial-and-fungal-infections-in-vivo
#5
Eleonora Ciarlo, Tytti Heinonen, Jacobus Herderschee, Craig Fenwick, Matteo Mombelli, Didier Le Roy, Thierry Roger
Short chain fatty acids (SCFAs) produced by intestinal microbes mediate anti-inflammatory effects, but whether they impact on antimicrobial host defenses remains largely unknown. This is of particular concern in light of the attractiveness of developing SCFA-mediated therapies and considering that SCFAs work as inhibitors of histone deacetylases which are known to interfere with host defenses. Here we show that propionate, one of the main SCFAs, dampens the response of innate immune cells to microbial stimulation, inhibiting cytokine and NO production by mouse or human monocytes/macrophages, splenocytes, whole blood and, less efficiently, dendritic cells...
November 29, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27870737/the-influence-of-body-mass-index-on-sensorimotor-block-and-vasopressor-requirement-during-spinal-anesthesia-for-elective-cesarean-delivery
#6
T C Ngaka, J F Coetzee, R A Dyer
BACKGROUND: It has been suggested that the dose requirement for spinal anesthesia (SA) is lower in obese patients for cesarean delivery (CD). In this prospective, observational, noninferiority study, we tested the hypothesis that obesity would not have a clinically important effect on vasopressor requirements or block height. METHODS: Two groups of 25 parturients, group O (body mass index [BMI] >40 kg/m) and group N (BMI <32 kg/m) requiring elective CD were recruited...
December 2016: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/27869499/capsaicinoids-improve-egg-production-by-regulating-ovary-nuclear-transcription-factors-against-heat-stress-in-quail
#7
N Sahin, C Orhan, M Tuzcu, V Juturu, K Sahin
1. To examine the molecular mechanism of capsaicinoid supplementation from Capsicum extract, laying Japanese quail (n=180, 5 weeks old) were reared either at 22(o)C for 24 h/d (thermoneutral, TN) or 34(o)C for 8 h/d (heat stress, HS) and fed on one of three diets containing 0, 25 or 50 mg of capsaicinoids per kg for 12 weeks (2x3 factorial arrangement). 2. The results revealed that exposure to HS decreased feed consumption by 10.7% and egg production by 13.6%, increased serum and ovary malondialdehyde (MDA) levels by 66...
November 21, 2016: British Poultry Science
https://www.readbyqxmd.com/read/27869107/studies-on-the-potential-risk-of-amyloidosis-from-exposure-to-silk-fibroin
#8
Satomi Tsukawaki, Tomoaki Murakami, Kazuhiko Suzuki, Yasumoto Nakazawa
Amyloid A (AA) amyloidosis can be induced by the administration of amyloid fibrils to animals under inflammatory conditions. Silk fibroin (SF) is a main component protein of bombic silk and has amyloid-like features. The amyloidogenesis of SF solution in mice has been previously reported. Recently, the biochemical properties of silk have attracted increasing attention, and research and development have been undertaken regarding applications other than as a clothing material. However, the risk of AA amyloidosis from exposure to SF-related products is unknown...
November 21, 2016: Biomedical Materials
https://www.readbyqxmd.com/read/27868250/effects-of-astragalus-membranaceus-codonopsis-pilosula-and-allicin-mixture-on-growth-performance-nutrient-digestibility-faecal-microbial-shedding-immune-response-and-meat-quality-in-finishing-pigs
#9
R X Lan, J W Park, D W Lee, I H Kim
A 12-week trial with 120 [(Landrace×Yorkshire)×Duroc] pigs (45.65 ± 1.93 kg) was conducted to evaluate the effects of Astragalus membranaceus, Codonopsis pilosula and allicin mixture (HM) supplementation on growth performance, nutrient digestibility, faecal microbial shedding, immune response and meat quality in finishing pigs. Pigs were allocated to one of three treatments with 0, 0.025% (HM1) and 0.05% (HM2) HM supplementation in a randomized complete block design according to sex and BW. Each treatment contained 10 replications with four pigs (two barrows and two gilts) per pen...
November 21, 2016: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/27866356/effect-of-feeding-and-withdrawal-of-vanadium-and-vitamin-c-on-egg-quality-and-vanadium-residual-over-time-in-laying-hens
#10
J P Wang, K R He, X M Ding, S P Bai, Q F Zeng, K Y Zhang
This experiment examined the egg quality of hens fed vanadium (V) and vitamin C (VC) during storage, as well as the V and VC withdrawal on egg quality and V residual in egg. A total of 360 laying hens (31 weeks old) were randomly allotted into a 3 × 2 factorial arrangement treatments (6 replicates and 10 chicks per replicate) with three levels of dietary V (0, 5, and 10 mg/kg) and two levels of VC (0 and 100 mg/kg) for 19 weeks (feeding V and VC 12 weeks, recovery 7 weeks). The V residual in eggs at 4, 8, and 12th weeks were increased (linear effect, P ≤ 0...
November 19, 2016: Biological Trace Element Research
https://www.readbyqxmd.com/read/27865904/surprises-of-electron-microscopic-imaging-of-proteins-and-polymers-covering-gold-nanoparticles-layer-by-layer
#11
Inna A Pyshnaya, Kristina V Razum, Anton S Dolodoev, Valeriya V Shashkova, Elena I Ryabchikova
Gold nanoparticles (GNPs) are used in complicated nanoconstructions, and their preparation implies careful analysis of the intermediate and resulting products, including visualisation of the NPs. Visualisation of protein and/or organic polymer covers on GNPs using electron microscopy (EM) was a goal of this study. We covered GNPs with human serum albumin or PEG, and then added a second layer of branched or linear polyethyleneimine. EM studies were supplemented with dynamic light scattering, spectrophotometry and gel electrophoresis, which confirmed the presence and integrity of a cover on GNPs in mixtures with uranylacetate (UA) or phosphotungstic acid (PTA)...
November 5, 2016: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/27862297/histochemical-localization-of-zinc-in-the-retina-cells-of-gilthead-sea-bream-sparus-aurata-fed-different-presentations-of-zinc
#12
Pedro Luis Castro, David Dominguez, María José Caballero, Marisol Izquierdo
To describe the distribution of zinc in the retina of a representative marine fish species and to determine whether the intracellular deposition amount correlates with the presentation of the zinc included in the practical diets (organic, inorganic, encapsulated, fish meal, and Control diet), we examined the precise localization of endogenous zinc in the gilthead sea bream (Sparus aurata) retina by autometallography. As observed by light microscopy, reaction products were widely distributed throughout the retina, including the outer segments of photoreceptors, except in the nuclear layers...
November 8, 2016: Synapse
https://www.readbyqxmd.com/read/27861558/modulation-of-type-1-and-type-2-cannabinoid-receptors-by-saffron-in-a-rat-model-of-retinal-neurodegeneration
#13
Rita Maccarone, Cinzia Rapino, Darin Zerti, Monia di Tommaso, Natalia Battista, Stefano Di Marco, Silvia Bisti, Mauro Maccarrone
Experimental studies demonstrated that saffron (Crocus sativus) given as a dietary supplement counteracts the effects of bright continuous light (BCL) exposure in the albino rat retina, preserving both morphology and function and probably acting as a regulator of programmed cell death [1]. The purpose of this study was to ascertain whether the neuroprotective effect of saffron on rat retina exposed to BCL is associated with a modulation of the endocannabinoid system (ECS). To this aim, we used eight experimental groups of Sprague-Dawley rats, of which six were exposed to BCL for 24 hours...
2016: PloS One
https://www.readbyqxmd.com/read/27858383/effect-of-creatine-on-rat-sciatic-nerve-injury-a-comparative-ultrastructural-study
#14
Fatma Helvacioglu, Ersin Kandemir, Büşra Karabacak, İdil Karataş, Ahmet Peçen, İpek Ercan, Tuğçe Şençelikel, Attila Dağdeviren
AIM: Creatine is an endogenous molecule synthesized in liver, kidney and pancreas from glycin and arginine which is important for mitochondrial metabolism. It is widely used as a supplement for improving muscle mass and function for many years. As it is expected to prevent apoptosis and diminish oxidative stress it is also studied in a number of neurodegenerative diseases for its beneficial effect in recent years. We studied the effect of creatine on the peripheral nerve injury in an experimental rat crush injury model to obtain ultrastructural evidence...
October 13, 2016: Turkish Neurosurgery
https://www.readbyqxmd.com/read/27857944/macular-carotenoid-supplementation-improves-disability-glare-performance-and-dynamics-of-photostress-recovery
#15
James M Stringham, Kevin J O'Brien, Nicole T Stringham
BACKGROUND: The so-called macular carotenoids (MC) lutein (L), zeaxanthin (Z), and meso-zeaxanthin (MZ) comprise the diet-derived macular pigment (MP). The purpose of this study was to determine effects of MC supplementation on the optical density of MP (MPOD), repeated-exposure photostress recovery (PSR), and disability glare (DG) thresholds. METHODS: This was a double-blind, placebo-controlled trial. Fifty-nine young (mean age = 21.7), healthy volunteers participated in this study...
2016: Eye and Vision (London, England)
https://www.readbyqxmd.com/read/27845437/production-of-selenium-nanoparticles-in-pseudomonas-putida-kt2440
#16
Roberto Avendaño, Nefertiti Chaves, Paola Fuentes, Ethel Sánchez, Jose I Jiménez, Max Chavarría
Selenium (Se) is an essential element for the cell that has multiple applications in medicine and technology; microorganisms play an important role in Se transformations in the environment. Here we report the previously unidentified ability of the soil bacterium Pseudomonas putida KT2440 to synthesize nanoparticles of elemental selenium (nano-Se) from selenite. Our results show that P. putida is able to reduce selenite aerobically, but not selenate, to nano-Se. Kinetic analysis indicates that, in LB medium supplemented with selenite (1 mM), reduction to nano-Se occurs at a rate of 0...
November 15, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27833594/candida-species-rewired-hyphae-developmental-programs-for-chlamydospore-formation
#17
Bettina Böttcher, Christine Pöllath, Peter Staib, Bernhard Hube, Sascha Brunke
Chlamydospore formation is a characteristic of many fungal species, among them the closely related human-pathogenic dimorphic yeasts Candida albicans and C. dubliniensis. Whereas function and regulation of filamentation are well-studied in these species, the basis of chlamydospore formation is mostly unknown. Here, we investigate the contribution of environmental and genetic factors and identified central proteins involved in species-specific regulation of chlamydosporulation. We show that specific nutrient levels strongly impact chlamydospore initiation, with starvation favoring sporulation and elevated levels of saccharides or peptone inhibiting it...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27827949/carbohydrate-supplementation-influences-serum-cytokines-after-exercise-under-hypoxic-conditions
#18
Aline Venticinque Caris, Edgar Tavares Da Silva, Samile Amorim Dos Santos, Fabio Santos Lira, Lila Missae Oyama, Sergio Tufik, Ronaldo Vagner Thomatieli Dos Santos
INTRODUCTION: Exercise performed at the hypoxia equivalent of an altitude of 4200 m is associated with elevated inflammatory mediators and changes in the Th1/Th2 response. By contrast, supplementation with carbohydrates has an anti-inflammatory effect when exercise is performed under normoxic conditions. The objective of this study was to evaluate the effect of carbohydrate supplementation on cytokines and cellular damage markers after exercise under hypoxic conditions at a simulated altitude of 4200 m...
November 5, 2016: Nutrients
https://www.readbyqxmd.com/read/27826818/a-qualitative-study-of-motivations-for-minority-recruitment-in-cancer-clinical-trials-across-five-nci-designated-cancer-centers
#19
Zachary R Simoni, Michelle Martin, Jennifer A Wenzel, Elise D Cook, Badrinath Konety, Selwyn M Vickers, Moon S Chen, Mona N Foaud, Raegan W Durant
BACKGROUND: Minority enrollment in cancer clinical trials is traditionally low. In light of this fact, numerous studies have investigated barriers to recruitment and retention within minority populations. However, very little research has investigated the importance of clinicians' and researchers' motivations for minority recruitment in cancer clinical trials. Therefore, we sought to examine motivations for minority recruitment across four professional stakeholder groups (principal investigators, clinicians, research staff, and Cancer Center leaders) at five National Cancer Institute (NCI)-designated Comprehensive Cancer Centers...
November 8, 2016: Journal of Racial and Ethnic Health Disparities
https://www.readbyqxmd.com/read/27824104/degeneration-and-energy-shortage-in-the-suprachiasmatic-nucleus-underlies-the-circadian-rhythm-disturbance-in-apoe-mice-implications-for-alzheimer-s-disease
#20
Lan Zhou, Qian Gao, Meng Nie, Jing-Li Gu, Wei Hao, Lin Wang, Ji-Min Cao
Alzheimer's disease (AD) patients suffer sleep disorders and circadian rhythm disturbances (CRDs). The underlying mechanisms are incompletely understood, and treatments are lacking. In this study, we characterized the locomotor activity, clock gene expression, morphological degeneration and energy metabolism of suprachiasmatic nucleus (SCN), together with retinal light sensing, in ApoE(-/-) mice, a model for AD. Compared with the control C57BL/6J mice, ApoE(-/-) mice exhibited disordered circadian locomotor activity under dim light and constant darkness, with impaired re-entrainment to phase change schedules...
November 8, 2016: Scientific Reports
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