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https://www.readbyqxmd.com/read/28722264/ultrastructure-changes-in-buffalo-bubalus-bubalis-oocytes-before-and-after-maturation-in%C3%A2-vitro-with-sericin
#1
Sri Gustina, Hasbi Hasbi, Ni Wayan Kurniani Karja, Mohamad Agus Setiadi, Iman Supriatna
The aim of this research was to identify the changes in the cytoplasmic ultrastructure of immature and matured oocytes in buffalo (Bubalus bubalis). Oocytes were matured in vitro in tissue culture medium-199 with and without sericin, and then analyzed by light and transmission electron microscopy. The experiment result showed that the nuclear maturation rate of buffalo oocytes was significantly higher in the presence of sericin (80.6%) than without sericin (68.1%) (P < 0.05). The immature oocytes were characterized by cortical granule clusters in the ooplasm and the absence of perivitelline space (PVS)...
July 19, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28715781/effects-of-blue-or-violet-light-on-the-inactivation-of-staphylococcus-aureus-by-riboflavin-5-phosphate-photolysis
#2
Tak-Wah Wong, Chien-Wei Cheng, Zong-Jhe Hsieh, Ji-Yuan Liang
The light sensitive compound riboflavin-5'-phosphate (or flavin mononucleotide, FMN) generates reactive oxygen species (ROS) upon photo-irradiation. FMN is required by all flavoproteins because it is a cofactor of biological blue-light receptors. The photochemical effects of FMN after irradiation by blue or violet light on the inactivation of Staphylococcus aureus strains, including a methicillin-resistant strain (MRSA), were investigated in this study. Upon blue- or violet-light photo-treatment, FMN was shown to inactivate S...
July 12, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28715644/detection-and-identification-of-serum-protein-peak-at-6648%C3%A2-m-z-as-a-novel-indicator-in-breast-cancer-based-on-mass-spectrometry
#3
Dongjian Song, Lifang Yue, Yuxiao Zhan, Junjie Zhang, Zechen Yan, Yingzhong Fan, Heying Yang, Da Zhang, Qiuliang Liu, Ziqiang Xia, Pan Qin, Jia Jia, Ming Yue, Jiekai Yu, Shu Zheng, Fuquan Yang, Jiaxiang Wang
Breast cancer (BC) is the second-leading cause of cancer mortality after lung cancer in women owing partly to a lack of specific and sensitive tests for early screening and monitoring. The detection of novel specific BC serum indicators for screening purposes is an essential clinical need. A total of 437 serum specimens from 310 BC patients that were divided into mining and testing sets were collected in this study. In contrast with the conventional BC indicators through receiver operating characteristic, survival and hazard function curves, and multivariate Cox regression analyses, we intended to hunt for stable protein indicators from serum specimens and identify their diagnostic and prognostic potential for BC...
May 2017: Discovery Medicine
https://www.readbyqxmd.com/read/28713382/recent-advances-on-nutrition-in-treatment-of-acute-pancreatitis
#4
REVIEW
Li-Long Pan, Jiahong Li, Muhammad Shamoon, Madhav Bhatia, Jia Sun
Acute pancreatitis (AP) is a common abdominal acute inflammatory disorder and the leading cause of hospital admission for gastrointestinal disorders in many countries. Clinical manifestations of AP vary from self-limiting local inflammation to devastating systemic pathological conditions causing significant morbidity and mortality. To date, despite extensive efforts in translating promising experimental therapeutic targets in clinical trials, disease-specific effective remedy remains obscure, and supportive care has still been the primary treatment for this disease...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28708059/a-tunable-refractive-index-matching-medium-for-live-imaging-cells-tissues-and-model-organisms
#5
Tobias Boothe, Lennart Hilbert, Michael Heide, Lea Berninger, Wieland B Huttner, Vasily Zaburdaev, Nadine L Vastenhouw, Eugene W Myers, David N Drechsel, Jochen C Rink
In light microscopy, refractive index mismatches between media and sample cause spherical aberrations that often limit penetration depth and resolution. Optical clearing techniques can alleviate these mismatches, but they are so far limited to fixed samples. We present Iodixanol as a non-toxic medium supplement that allows refractive index matching in live specimens and thus a substantial improvement of the live-imaging of primary cell cultures, planarians, zebrafish and human cerebral organoids.
July 14, 2017: ELife
https://www.readbyqxmd.com/read/28704190/modulating-effects-of-cholecalciferol-treatment-on-estrogen-deficiency-induced-anxiety-like-behavior-of-adult-female-rats
#6
Julia Fedotova, Daria Zarembo, Jozef Dragasek, Martin Caprnda, Peter Kruzliak, Tatyana Dudnichenko
BACKGROUND: Vitamin D can be one of the candidate substances that are used as additional supplementation in the treatment of anxiety-related disorders in women with estrogen imbalance. MATERIALS AND METHODS: The aim of the present study was to examine the effects of chronic cholecalciferol administration (1.0, 2.5 or 5.0 mg/kg/day, s.c.) on the anxiety-like behavior and monoamines levels in the rat hippocampus following ovariectomy in female rats. Cholecalciferol was given to ovariectomized (OVX) rats and OVX rats treated with 17β-estradiol (17β-E2, 0...
June 1, 2017: Folia Medica
https://www.readbyqxmd.com/read/28702425/differential-effects-of-cod-proteins-and-tuna-proteins-on-serum-and-liver-lipid-profiles-in-rats-fed-non-cholesterol-and-cholesterol-containing-diets
#7
Ryota Hosomi, Hayato Maeda, Yuki Ikeda, Yuko Toda, Munehiro Yoshida, Kenji Fukunaga
Fish muscles are classified into white and red muscles, and the chemical composition of the two fish muscles have many differences. Few reports have assessed the health-promoting functions of white fish muscle proteins (WFP) and red fish muscle proteins (RFP). We therefore evaluated the mechanisms underlying the alteration of lipid profiles and cholesterol metabolism following the intake of WFP prepared from cod and RFP prepared from light muscles of tuna. Male Wistar rats were divided into six dietary groups: casein (23%), WFP (23%), and RFP (23%), with or without 0...
June 2017: Preventive Nutrition and Food Science
https://www.readbyqxmd.com/read/28701241/arginine-metabolism-and-its-protective-effects-on-intestinal-health-and-functions-in-weaned-piglets-under-oxidative-stress-induced-by-diquat
#8
Ping Zheng, Bing Yu, Jun He, Jie Yu, Xiangbing Mao, Yuheng Luo, Junqiu Luo, Zhiqing Huang, Gang Tian, Qiufeng Zeng, Lianqiang Che, Daiwen Chen
The intestine plays key roles in maintaining body arginine (Arg) homoeostasis. Meanwhile, the intestine is very susceptible to reactive oxygen species. In light of this, the study aimed to explore the effects of Arg supplementation on intestinal morphology, Arg transporters and metabolism, and the potential protective mechanism of Arg supplementation in piglets under oxidative stress. A total of thirty-six weaned piglets were randomly allocated to six groups with six replicates and fed a base diet (0·95 % Arg,) or base diet supplemented with 0·8 % and 1·6 % l-Arg for 1 week, respectively...
July 13, 2017: British Journal of Nutrition
https://www.readbyqxmd.com/read/28697225/low-dose-methotrexate-for-vitiligo
#9
Anna Cristina Garza-Mayers, Daniela Kroshinsky
<p>BACKGROUND: Treatment of vitiligo is aimed at repigmentation and often consists of multiple modalities, none of which are universally or rapidly successful. Extensive cases are most often treated with ultraviolet light therapy, which can be both costly and time-consuming. Though vitiligo is an autoimmune disease, there is no current data to support systemic immunosuppressive monotherapy.</p> <p>CASE SUMMARY: Here we present a case series of 3 patients with vitiligo treated for 11-16 months with low-dose methotrexate (12...
July 1, 2017: Journal of Drugs in Dermatology: JDD
https://www.readbyqxmd.com/read/28693194/combination-of-lenalidomide-and-low-dose-dexamethasone-therapy-promotes-the-anticoagulant-activity-of-warfarin-in-patients-with-immunoglobulin-light-chain-amyloidosis
#10
Fumiaki Kitazawa, Shin-Ichi Fuchida, Fumitaka Ise, Yoko Kado, Kumi Ueda, Takatoshi Kokufu, Akira Okano, Mayumi Hatsuse, Satoshi Murakami, Yuko Nakayama, Kohji Takara, Chihiro Shimazaki
The present study aimed to evaluate the drug interactions between warfarin and combination chemotherapy with lenalidomide and low-dose dexamethasone in immunoglobulin light-chain (AL) amyloidosis patients with unstable international normalized ratios (INR). The changes to INR values over time in 3 AL amyloidosis patients treated with warfarin and a combination of lenalidomide and low-dose dexamethasone between March 2011 and February 2015 were analyzed retrospectively. The mean INR value was 1.52 prior to the combination chemotherapy, and the value increased 1...
July 2017: Oncology Letters
https://www.readbyqxmd.com/read/28689556/exploring-the-potential-for-technology-based-nutrition-education-among-wic-recipients-in%C3%A2-remote-alaska-native-communities
#11
Julianne M Power, Kathryn L Braun, Andrea Bersamin
OBJECTIVE: Estimate media technology use in Alaska Native communities to inform the feasibility of technology-based nutrition education. METHODS: A self-administered questionnaire was mailed to a random selection of about 50% of Special Supplemental Nutrition Program for Women, Infants, and Children (WIC) authorized representatives in remote Alaska Native communities (n = 975). Media technology use, interest in media technology-based nutrition education, and potential barriers were assessed...
July 2017: Journal of Nutrition Education and Behavior
https://www.readbyqxmd.com/read/28676816/the-effect-of-spectral-quality-on-daily-patterns-of-gas-exchange-biomass-gain-and-water-use-efficiency-in-tomatoes-and-lisianthus-an-assessment-of-whole-plant-measurements
#12
Jason Lanoue, Evangelos D Leonardos, Xiao Ma, Bernard Grodzinski
Advancements in light-emitting diode (LED) technology have made them a viable alternative to current lighting systems for both sole and supplemental lighting requirements. Understanding how wavelength specific LED lighting can affect plants is thus an area of great interest. Much research is available on the wavelength specific responses of leaves from multiple crops when exposed to long-term wavelength specific lighting. However, leaf measurements do not always extrapolate linearly to the complexities which are found within a whole plant canopy, namely mutual shading and leaves of different ages...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28676009/aging-eye-microbiota-in-dry-eye-syndrome-in-patients-treated-with-enterococcus-faecium-and-saccharomyces-boulardii
#13
Giuseppe Chisari, Eleonora Margherita Chisari, Antonio Maria Borzì, Erdogan Ozyalcin, Clara Grazia Chisari
Aging seem to have a key role in the onset and progression of ocular surface diseases. Dry Eye Syndrome (DES) is a multifactorial disease of the tears and ocular surface in which symptoms may interfere with the ability to work and carry out daily functions. METHODS: This clinical trial was a pilot study to evaluate the effects of supplementation with mixture (MYA796 and Enterococcus faecium SGEf01) on the tear film. Following the run-in period subjects were randomized in two groups: group A (n...
July 4, 2017: Current Clinical Pharmacology
https://www.readbyqxmd.com/read/28674599/the-effect-of-betaine-on-nitrate-and-cardiovascular-response-to-exercise
#14
J Luke Pryor, S Tony Wolf, Gary Sforzo, Tom Swensen
Betaine (BT) supplementation improves selected markers of physical performance, however, the mechanism(s) by which this change occurs remains largely unknown. Some speculate that BT may increase circulating nitrate concentrations, improving physical performance by augmentation of endothelial nitric oxide production. The purpose of this study was to investigate the effects of acute BT supplementation and exercise on plasma nitrate levels and related cardiovascular response (CVR). Placebo and BT trials were administered in a cross-over, randomized, double-blind, and counterbalanced fashion...
2017: International Journal of Exercise Science
https://www.readbyqxmd.com/read/28671569/influence-of-environmental-factors-on-the-production-of-penitrems-a-f-by-penicillium-crustosum
#15
Svetlana A Kalinina, Annika Jagels, Benedikt Cramer, Rolf Geisen, Hans-Ulrich Humpf
Filamentous fungi produce a multitude of secondary metabolites, some of them known as mycotoxins, which are toxic to vertebrates and other animal groups in low concentrations. Among them, penitrems, which belong to the group of indole-diterpene mycotoxins, are synthesized by Penicillium and Aspergillus genera and exhibit potent tremorgenic effects. This is the first complex study of the penitrems A-F production under the influence of different abiotic factors, e.g., media, incubation time, temperature, pH, light, water activity, and carbon and nitrogen source as well as oxidative and salt stress...
July 1, 2017: Toxins
https://www.readbyqxmd.com/read/28663826/a-practical-application-of-photogrammetry-to-performing-rib-characterization-measurements-in-an-underground-coal-mine-using-a-dslr-camera
#16
Brent A Slaker, Khaled M Mohamed
Understanding coal mine rib behavior is important for inferring pillar loading conditions as well as ensuring the safety of miners who are regularly exposed to ribs. Due to the variability in the geometry of underground openings and ground behavior, point measurements often fail to capture the true movement of mine workings. Photogrammetry is a potentially fast, cheap, and precise supplemental measurement tool in comparison to extensometers, tape measures, or laser range meters, but its application in underground coal has been limited...
January 2017: International Journal of Mining Science and Technology
https://www.readbyqxmd.com/read/28661438/macular-carotenoid-supplementation-improves-visual-performance-sleep-quality-and-adverse-physical-symptoms-in-those-with-high-screen-time-exposure
#17
James M Stringham, Nicole T Stringham, Kevin J O'Brien
The dramatic rise in the use of smartphones, tablets, and laptop computers over the past decade has raised concerns about potentially deleterious health effects of increased "screen time" (ST) and associated short-wavelength (blue) light exposure. We determined baseline associations and effects of 6 months' supplementation with the macular carotenoids (MC) lutein, zeaxanthin, and mesozeaxanthin on the blue-absorbing macular pigment (MP) and measures of sleep quality, visual performance, and physical indicators of excessive ST...
June 29, 2017: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28660095/development-of-the-fitsight-fitness-tracker-to-increase-time-outdoors-to-prevent-myopia
#18
Pavan K Verkicharla, Dharani Ramamurthy, Quang Duc Nguyen, Xinquan Zhang, Suan-Hui Pu, Rahul Malhotra, Truls Ostbye, Ecosse L Lamoureux, Seang-Mei Saw
PURPOSE: To develop a fitness tracker (FitSight) to encourage children to increase time spent outdoors. To evaluate the wear pattern for this tracker and outdoor time pattern by estimating light illumination levels among children. METHODS: The development of the FitSight fitness tracker involved the designing of two components: (1) the smartwatch with custom-made FitSight watch application (app) to log the instant light illuminance levels the wearer is exposed to, and (2) a companion smartphone app that synchronizes the time outdoors recorded by the smartwatch to smartphone via Bluetooth communication...
June 2017: Translational Vision Science & Technology
https://www.readbyqxmd.com/read/28656576/carbon-dioxide-biofixation-and-phycoremediation-of-municipal-wastewater-using-chlorella-vulgaris-and-scenedesmus-obliquus
#19
Ramjee Chaudhary, Anil Kumar Dikshit, Yen Wah Tong
The pure cultures of microalgae Chlorella vulgaris ATCC 13482 and Scenedesmus obliquus FACHB 417 were grown in municipal wastewater in 7-L airlift bubble column photobioreactor supplied with 5% CO2/air (v/v). Batch experiments were conducted at 25 °C with 14-h light/10-h dark cycle for a period of 10 days. The CO2 capture efficiencies for both the microalgae were monitored in terms of their respective biomass productivities, carbon contents, and CO2 consumption rates. In the present study, the initial concentration of ammonia (43...
June 27, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28655697/banana-fruit-pulp-and-peel-involved-in-antianxiety-and-antidepressant-effects-while-invigorate-memory-performance-in-male-mice-possible-role-of-potential-antioxidants
#20
Noreen Samad, Aqsa Muneer, Najeeb Ullah, Aqal Zaman, M Mazhar Ayaz, Ijaz Ahmad
The present study was aimed to investigate the anti-stress and memory enhancing effects of banana (Musa sapientum L.) fruit pulp and peel extract in male mice. Locally bred albino Wistar mice were divided into control and 2 test groups (n=10). Control rats received drinking water while test groups were treated with banana fruit pulp (600 mg/kg; oral administration) and extract of banana peel (400mg/kg; oral administration). Behavioral activities of animals were monitored 14 days post administration of banana pulp and peel extract...
May 2017: Pakistan Journal of Pharmaceutical Sciences
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