Estelle Marrer-Berger, Annalisa Nicastri, Angelique Augustin, Vesna Kramar, Hanqing Liao, Lydia Jasmin Hanisch, Alejandro Carpy, Tina Weinzierl, Evelyne Durr, Nathalie Schaub, Ramona Nudischer, Daniela Ortiz-Franyuti, Ekaterina Breous-Nystrom, Janick Stucki, Nina Hobi, Giulia Raggi, Lauriane Cabon, Emmanuelle Lezan, Pablo Umaña, Isaac Woodhouse, Alexander Bujotzek, Christian Klein, Nicola Ternette
Selective binding of TCR-like antibodies that target a single tumour-specific peptide antigen presented by human leukocyte antigens (HLA) is the absolute prerequisite for their therapeutic suitability and patient safety. To date, selectivity assessment has been limited to peptide library screening and predictive modeling. We developed an experimental platform to de novo identify interactomes of TCR-like antibodies directly in human tissues using mass spectrometry. As proof of concept, we confirm the target epitope of a MAGE-A4-specific TCR-like antibody...
April 16, 2024: Nature Communications