Read by QxMD icon Read

Neisseria gonorrhoeae

Manju Bala, Vikram Singh, Aradhana Bhargava, Monika Kakran, Naveen Chandra Joshi, Ravi Bhatnagar
Antimicrobial susceptibility testing of 258 N. gonorrhoeae isolates by Etest determined that 60.1% were MDR while 5% strains had decreased susceptibility to currently recommended extended-spectrum cephalosporins (ESCs). Among these, 84.5% MDR and 76.9% strains having decreased susceptibility to ESCs were susceptible to gentamicin. No MDR isolate was resistant to gentamicin. These in vitro results suggest that gentamicin might be an effective treatment option for the MDR strains and in dual therapy for gonorrhea...
October 10, 2016: Antimicrobial Agents and Chemotherapy
Carolien M Wind, Maarten F Schim van der Loeff, Magnus Unemo, Rob Schuurman, Alje P van Dam, Henry J C de Vries
BACKGROUND: Performing a test of cure (TOC) could demonstrate success or failure of antimicrobial treatment of Chlamydia trachomatis infection, but recommendations for the timing of a TOC using nucleic acid amplification tests (NAATs) are inconsistent. We assessed time to clearance of C. trachomatis after treatment, using modern RNA- and DNA-based NAATs. METHODS: We analysed data from patients with a C. trachomatis and Neisseria gonorrhoeae coinfection who visited the STI Clinic Amsterdam, The Netherlands, from March through October 2014...
October 11, 2016: BMC Infectious Diseases
Pushpa Bhawan Mal, Kauser Jabeen, Joveria Farooqi, Magnus Unemo, Erum Khan
BACKGROUND: Accurate detection of Neisseria gonorrhoeae antimicrobial resistance is essential for appropriate management and prevention of spread of infection in the community. In this study Calibrated Dichotomous Sensitivity (CDS) and Clinical Laboratory Standards Institute (CLSI) disc diffusion methods were compared with minimum inhibitory concentration (MIC) by Etest in Neisseria gonorrhoeae isolates from Karachi, Pakistan. CDS and CLSI disc diffusion techniques, and Etest for ceftriaxone, penicillin G, spectinomycin and ciprofloxacin against 100 isolates from years 2012-2014 were performed...
October 10, 2016: BMC Microbiology
Namraj Goire, Ratan Kundu, Ella Trembizki, Cameron Buckley, Tiffany R Hogan, David A Lewis, James M Branley, David M Whiley, Monica M Lahra
OBJECTIVES: Previous studies have shown that mixed-strain gonococcal infections can occur. However, it remains unclear whether such infections impact upon the reliability of Neisseria gonorrhoeae antimicrobial resistance (AMR) surveillance. In this study, we aimed to resolve this question by intensively sampling isolates from gonorrhoea-positive specimens in a high-risk population in Sydney, Australia. METHODS: A total of 615 N. gonorrhoeae isolates, originating from 63 clinical samples (31 rectal swabs and 32 throat swabs), were characterized...
October 5, 2016: Journal of Antimicrobial Chemotherapy
R Somayaji, C Naugler, M Guo, D Church
Bacterial sexually transmitted infections including Chlamydia trachomatis and Neisseria gonorrhoeae remain an important public health concern. We aimed to assess the population-based incidence of C. trachomatis and N. gonorrhoeae in an age-standardized cohort over time. A retrospective study of a large Canadian health region was undertaken between 2010 and 2015 using linked census and digital laboratory data. C. trachomatis and N. gonorrhoeae tests were linked to patient data. Sex and age-standardized incidence rates (IR) and ratios (IRR) were calculated for cases and testing rates...
October 5, 2016: International Journal of STD & AIDS
Charifa Zemouri, Teodora Elvira Wi, James Kiarie, Armando Seuc, Vittal Mogasale, Ahmed Latif, Nathalie Broutet
BACKGROUND: This review aimed to synthesize and analyze the diagnostic accuracy and the likelihood of providing correct treatment of the syndromic approach Vaginal Discharge Flowchart in managing cervical infections caused by Neisseria gonorrhoeae (NG) and Chlamydia trachomatis (CT), and vaginal infections caused by Trichomonas vaginalis (TV) and Bacterial vaginosis (BV) and Candida albicans. This review will inform updating the WHO 2003 guidelines on Vaginal Discharge syndromic case management...
2016: PloS One
André Zapun, Cécile Morlot, Muhamed-Kheir Taha
Neisseria meningitidis and Neisseria gonorrhoeae are human pathogens that cause a variety of life-threatening systemic and local infections, such as meningitis or gonorrhoea. The treatment of such infection is becoming more difficult due to antibiotic resistance. The focus of this review is on the mechanism of reduced susceptibility to penicillin and other β-lactams due to the modification of chromosomally encoded penicillin-binding proteins (PBP), in particular PBP2 encoded by the penA gene. The variety of penA alleles and resulting variant PBP2 enzymes is described and the important amino acid substitutions are presented and discussed in a structural context...
2016: Antibiotics
Ella Trembizki, Handan Wand, Basil Donovan, Marcus Chen, Christopher K Fairley, Kevin Freeman, Rebecca Guy, John M Kaldor, Monica M Lahra, Andrew Lawrence, Colleen Lau, Julie Pearson, David G Regan, Nathan Ryder, Helen Smith, Kerrie Stevens, Jiunn-Yih Su, James Ward, David M Whiley
BACKGROUND:  Antimicrobial resistance (AMR) by Neisseria gonorrhoeae (NG) is considered a serious global threat. METHODS:  In this nationwide study, we used MassARRAY iPLEX genotyping technology to examine the epidemiology of NG and associated AMR in the Australian population. All available NG isolates (n=2,452) received from Australian reference laboratories from January to June 2012 were included in the study. Genotypic data were combined with phenotypic AMR information to define strain-types...
September 28, 2016: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
Sabrina B Roberts, Russell Spencer-Smith, Mahwish Shah, Jean-Christophe Nebel, Richard T Cook, Lori A S Snyder
Neisseria gonorrhoeae is capable of causing gonorrhoea and more complex diseases in the human host. Neisseria meningitidis is a closely related pathogen that shares many of the same genomic features and virulence factors, but causes the life threatening diseases meningococcal meningitis and septicaemia. The importance of non-coding RNAs in gene regulation has become increasingly evident having been demonstrated to be involved in regulons responsible for iron acquisition, antigenic variation, and virulence. Neisseria spp...
August 25, 2016: Microorganisms
Alexandra Brunner, Eva Nemes-Nikodem, Csaba Jeney, Dora Szabo, Marta Marschalko, Sarolta Karpati, Eszter Ostorhazi
BACKGROUND: In the 1990s, azithromycin became the drug of choice for many infectious diseases but emerging resistance to the drug has only been reported in the last decade. In the last 5 years, the National Neisseria gonorrhoeae Reference Laboratory of Hungary (NNGRLH) has also observed an increased number of N. gonorrhoeae strains resistant to azithromycin. The aim of this study was to determine the most frequent sequence types (ST) of N. gonorrhoeae related to elevated levels of azithromycin MIC (minimal inhibitory concentration)...
2016: Annals of Clinical Microbiology and Antimicrobials
Sunniva Foerster, Magnus Unemo, Lucy J Hathaway, Nicola Low, Christian L Althaus
BACKGROUND: Gonorrhoea is a sexually transmitted infection caused by the Gram-negative bacterium Neisseria gonorrhoeae. Resistance to first-line empirical monotherapy has emerged, so robust methods are needed to evaluate the activity of existing and novel antimicrobials against the bacterium. Pharmacodynamic models describing the relationship between the concentration of antimicrobials and the minimum growth rate of the bacteria provide more detailed information than the MIC only. RESULTS: In this study, a novel standardised in vitro time-kill curve assay was developed...
2016: BMC Microbiology
Yonatan H Grad, Simon R Harris, Robert D Kirkcaldy, Anna G Green, Debora S Marks, Stephen D Bentley, David Trees, Marc Lipsitch
BACKGROUND:  Treatment of Neisseria gonorrhoeae infection is empiric and based on population-wide susceptibilities. Increasing antimicrobial resistance underscores the potential importance of rapid diagnostics, including sequence-based tests, to guide therapy. However, the utility of sequence-based diagnostics depends on the prevalence and dynamics of the resistance mechanisms. METHODS:  We define the prevalence and dynamics of resistance markers to extended spectrum cephalosporins (ESC), macrolides, and fluoroquinolones in 1102 resistant and susceptible clinical N...
September 16, 2016: Journal of Infectious Diseases
Thomas Regnath, Thomas Mertes, Ralf Ignatius
Increasing antimicrobial resistance of Neisseria gonorrhoeae, particularly to third-generation cephalosporins, has been reported in many countries. We examined the susceptibility (determined by Etest and evaluated using the breakpoints of the European Committee on Antimicrobial Susceptibility Testing) of 434 N. gonorrhoeae isolates collected from 107 female and 327 male patients in Stuttgart, south-west Germany, between 2004 and 2015. During the study period, high proportions of isolates were resistant to ciprofloxacin (70...
September 8, 2016: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
Jennifer Nall, Breona Barr, Candice J McNeil, Laura H Bachmann
From January 1, 2014, to May 31, 2015, 452 individuals received extragenital nucleic acid amplification-based Neisseria gonorrhoeae and Chlamydia trachomatis testing through public health venues. Seventy-four individuals (16%) tested positive for Neisseria gonorrhoeae and/or Chlamydia trachomatis at an extragenital site and 40 (54%) would not have been effectively diagnosed and treated in the absence of extragenital testing.
October 2016: Sexually Transmitted Diseases
Erik Munson, David Wenten, Sheila Jhansale, Mary Kay Schuknecht, Nicki Pantuso, Joshua Gerritts, Aaron Steward, Kimber L Munson, Maureen Napierala, Deb Hamer
Of 1493 male sexually-transmitted infection (STI) clinic encounters in a high-prevalence STI community, 8.7% revealed detection of Chlamydia trachomatis and 6.6% revealed detection of Neisseria gonorrhoeae Additional Trichomonas vaginalis and Mycoplasma genitalium screening resulted in 17.4% and 23.9% of encounters positive for STI, respectively. STI agents were detected in 13.7% of urine specimens; addition of pharyngeal and rectal collections resulted in detection of STI agents in 19.0% and 23.9% of encounters, respectively...
September 14, 2016: Journal of Clinical Microbiology
I Putu Yuda Hananta, Alje P van Dam, Sylvia Maria Bruisten, Maarten Franciscus Schim van der Loeff, Hardyanto Soebono, Henry John Christiaan de Vries
BACKGROUND: Little is known about the epidemiology of asymptomatic urogenital gonorrhea and antimicrobial susceptibility of Neisseria gonorrhoeae (NG) strains circulating in Indonesia. We studied these issues in 3 large Indonesian cities. METHODS: In 2014, participants were recruited from sexually transmitted infection clinics and through outreach in Jakarta, Yogyakarta, and Denpasar. Neisseria gonorrhoeae detection in genital specimens was performed with NG-qPCR at the Public Health Service in Amsterdam...
October 2016: Sexually Transmitted Diseases
Seada Ali, Tsegaye Sewunet, Zewdineh Sahlemariam, Gebre Kibru
BACKGROUND: Neisseria gonorrhoeae is a bacterium responsible for one of the classic sexually transmitted infection (STI) gonorrhea. Antibiotic resistant strains are emerging at alarming rate. Multiple sexual partners, unsafe sex and substance use habits are the main host related risk factors for acquiring the infection. Thus, this study aimed at determining the magnitude, its determinants and antimicrobial resistance profile of N. gonorrhoeae in a place where there is risk related cultural practices and relatively high HIV prevalence...
2016: BMC Research Notes
Amrita Bharat, Irene Martin, Walter Demczuk, Vanessa Allen, David Haldane, Linda Hoang, Michael R Mulvey
Neisseria gonorrhoeae strains with coresistance to the first-line antimicrobial treatments azithromycin and ceftriaxone are an emerging public health threat. Here, we present the complete genome sequences of three strains of N. gonorrhoeae, including one susceptible strain and two strains with coresistance to ceftriaxone and azithromycin.
2016: Genome Announcements
Ryan E Schaub, Yolande A Chan, Mijoon Lee, Dusan Hesek, Shahriar Mobashery, Joseph P Dillard
Neisseria gonorrhoeae releases peptidoglycan (PG) fragments during infection that provoke a large inflammatory response and, in pelvic inflammatory disease, this response leads to the death and sloughing of ciliated cells of the Fallopian tube. We characterized the biochemical functions and localization of two enzymes responsible for the release of proinflammatory PG fragments. The putative lytic transglycosylases LtgA and LtgD were shown to create the 1,6-anhydromuramyl moieties, and both enzymes were able to digest a small, synthetic tetrasaccharide dipeptide PG fragment into the cognate 1,6-anhydromuramyl-containing reaction products...
September 8, 2016: Molecular Microbiology
Stella Pendle, Timothy Barnes
We describe a case of Neisseria gonorrhoeae isolated from a nipple piercing wound in a homosexual man with pharyngeal gonococcal carriage.
September 9, 2016: Sexual Health
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"