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Lactoferrin

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https://www.readbyqxmd.com/read/29464457/cloning-and-characterization-of-the-human-lactoferrin-receptor-gene-promoter
#1
Rulan Jiang, Bo Lönnerdal
Lactoferrin (Lf) is a major protein in human milk. Multiple biological functions of Lf are postulated to be mediated by a Lf receptor (LfR). The Lf receptor (LfR) plays an important role in absorption of Lf and Lf-bound iron by intestinal epithelial cells. Here, we cloned and characterized the promoter from a ~ 3.1 kb 5'-flanking region of the human LfR gene. Neither a TATA box nor a CCAAT box is found at the typical positions. The transcription start site was identified as 298 bp upstream of the translation start codon (+ 1) by 5' RLM-RACE...
February 20, 2018: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
https://www.readbyqxmd.com/read/29462817/the-interaction-of-n-acetylcysteine-and-serum-transferrin-promotes-bacterial-biofilm-formation
#2
Supeng Yin, Bei Jiang, Guangtao Huang, Yulong Zhang, Bo You, Yu Chen, Yali Gong, Jing Chen, Zhiqiang Yuan, Yan Zhao, Ming Li, Fuquan Hu, Zichen Yang, Yizhi Peng
BACKGROUND/AIMS: N-acetylcysteine (NAC) is a novel and promising agent with activity against bacterial biofilms. Human serum also inhibits biofilm formation by some bacteria. We tested whether the combination of NAC and human serum offers greater anti-biofilm activity than either agent alone. METHODS: Microtiter plate assays and confocal laser scanning microscopy were used to evaluate bacterial biofilm formation in the presence of NAC and human serum. qPCR was used to examine expression of selected biofilm-associated genes...
February 15, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29455278/a-review-of-the-design-and-modification-of-lactoferricins-and-their-derivatives
#3
Ya Hao, Na Yang, Da Teng, Xiumin Wang, Ruoyu Mao, Jianhua Wang
Lactoferricin (Lfcin), a multifunction short peptide with a length of 25 residues, is derived from the whey protein lactoferrin by acidic pepsin hydrolysis. It has potent nutritional enhancement, antimicrobial, anticancer, antiviral, antiparasitic, and anti-inflammatory activities. This review describes the research advantages of the above biological functions, with attention to the molecular design and modification of Lfcin. In this examination of design and modification studies, research on the identification of Lfcin active derivatives and crucial amino acid residues is also reviewed...
February 17, 2018: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
https://www.readbyqxmd.com/read/29455251/establishing-and-characterizing-lacrispheres-from-human-lacrimal-gland-for-potential-clinical-application
#4
Shubha Tiwari, Rohini M Nair, Praseeda Vamadevan, Mohammad Javed Ali, Milind N Naik, Santosh G Honavar, Geeta K Vemuganti
PURPOSE: Lacrimal gland (LG) dysfunction leading to dry eye syndrome (DES) is an important cause of ocular morbidity. One of the potential and promising long-term management therapies for restoration of LG function could be transplantation of autologous ex vivo expanded stem cells. The present study was aimed at exploring the 2D and 3D cultures of human LG, identifying inherent stem cells and evaluating their secretory potential. METHODS: Fresh human lacrimal gland (HuLG) (n = 5) from patients undergoing therapeutic exenteration was harvested after ethical approval and informed consent...
February 17, 2018: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/29454662/fecal-markers-of-intestinal-inflammation-and-intestinal-permeability-are-elevated-in-parkinson-s-disease
#5
Andreas Schwiertz, Jörg Spiegel, Ulrich Dillmann, David Grundmann, Jan Bürmann, Klaus Faßbender, Karl-Herbert Schäfer, Marcus M Unger
BACKGROUND/OBJECTIVE: Intestinal inflammation and increased intestinal permeability (both possibly fueled by dysbiosis) have been suggested to be implicated in the multifactorial pathogenesis of Parkinson's disease (PD). The objective of the current study was to investigate whether fecal markers of inflammation and impaired intestinal barrier function corroborate this pathogenic aspect of PD. METHODS: In a case-control study, we quantitatively analyzed established fecal markers of intestinal inflammation (calprotectin and lactoferrin) and fecal markers of intestinal permeability (alpha-1-antitrypsin and zonulin) in PD patients (n = 34) and controls (n = 28, group-matched for age) by enzyme-linked immunosorbent assay...
February 12, 2018: Parkinsonism & related Disorders
https://www.readbyqxmd.com/read/29453656/the-ferroportin-ceruloplasmin-system-and-the-mammalian-iron-homeostasis-machine-regulatory-pathways-and-the-role-of-lactoferrin
#6
Maria Carmela Bonaccorsi di Patti, Antimo Cutone, Fabio Polticelli, Luigi Rosa, Maria Stefania Lepanto, Piera Valenti, Giovanni Musci
In the last 20 years, several new genes and proteins involved in iron metabolism in eukaryotes, particularly related to pathological states both in animal models and in humans have been identified, and we are now starting to unveil at the molecular level the mechanisms of iron absorption, the regulation of iron transport and the homeostatic balancing processes. In this review, we will briefly outline the general scheme of iron metabolism in humans and then focus our attention on the cellular iron export system formed by the permease ferroportin and the ferroxidase ceruloplasmin...
February 16, 2018: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
https://www.readbyqxmd.com/read/29450143/purified-horse-milk-exosomes-contain-an-unpredictable-small-number-of-major-proteins
#7
Sergey E Sedykh, Lada V Purvinish, Artem S Monogarov, Evgeniya E Burkova, Alina E Grigor'eva, Dmitrii V Bulgakov, Pavel S Dmitrenok, Valentin V Vlassov, Elena I Ryabchikova, Georgy A Nevinsky
Exosomes are 40-100 nm nanovesicles containing RNA and different proteins. Exosomes containing proteins, lipids, mRNAs, and microRNAs are important in intracellular communication and immune function. Exosomes from different sources are usually obtained by combination of centrifugation and ultracentrifugation and according to published data can contain from a few dozens to thousands of different proteins. Crude exosome preparations from milk of eighteen horses were obtained for the first time using several standard centrifugations...
June 2017: Biochimie Open
https://www.readbyqxmd.com/read/29442205/antibacterial-and-immunomodulatory-activities-of-bovine-lactoferrin-against-escherichia-coli-o157-h7-infections-in-cattle
#8
Evelien Kieckens, Joanna Rybarczyk, Eric Cox, Daisy Vanrompay
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a zoonotic pathogen that causes food-borne disease in humans ranging from watery diarrhea to bloody diarrhea and severe hemorrhagic colitis, renal failure and hemolytic uremic syndrome. Cattle, the most important source of E. coli O157:H7 transmission to humans, harbor the bacteria in their gastrointestinal tract without showing clinical symptoms. Prevention of E. coli O157:H7 infections in ruminants could diminish the public health risk. However, there is no specific treatment available nor a vaccine or a therapeutic agent which completely prevents E...
February 13, 2018: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
https://www.readbyqxmd.com/read/29440896/intranasal-delivery-of-huperzine-a-to-the-brain-using-lactoferrin-conjugated-n-trimethylated-chitosan-surface-modified-plga-nanoparticles-for-treatment-of-alzheimer-s-disease
#9
Qingqing Meng, Aiping Wang, Hongchen Hua, Ying Jiang, Yiyun Wang, Hongjie Mu, Zimei Wu, Kaoxiang Sun
Background: Safe and effective delivery of therapeutic drugs to the brain is important for successful therapy of Alzheimer's disease (AD). Purpose: To develop Huperzine A (HupA)-loaded, mucoadhesive and targeted polylactide-co-glycoside (PLGA) nanoparticles (NPs) with surface modification by lactoferrin (Lf)-conjugated N-trimethylated chitosan (TMC) (HupA Lf-TMC NPs) for efficient intranasal delivery of HupA to the brain for AD treatment. Methods: HupA Lf-TMC NPs were prepared using the emulsion-solvent evaporation method and optimized using the Box-Behnken design...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29436684/lactoferrin-promotes-murine-c2c12-myoblast-proliferation-and-differentiation-and-myotube-hypertrophy
#10
Tomoya Kitakaze, Meiku Oshimo, Yasuyuki Kobayashi, Mizuyuki Ryu, Yasushi A Suzuki, Hiroshi Inui, Naoki Harada, Ryoichi Yamaji
Lactoferrin (Lf) is a multifunctional glycoprotein, which promotes the proliferation of murine C2C12 myoblasts. In the present study, it was investigated how Lf promotes myoblast proliferation and whether Lf promotes myoblast differentiation or induces myotube hypertrophy. Lf promoted the proliferation of myoblasts in a dose‑dependent manner. Myoblast proliferation increased on day 3 when myoblasts were cultured in the presence of Lf for three days and also when myoblasts were cultured in the presence of Lf for the first day and in the absence of Lf for the subsequent two days...
February 13, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29435826/efficacy-of-bovine-lactoferrin-in-the-post-surgical-treatment-of-patients-suffering-from-bisphosphonate-related-osteonecrosis-of-the-jaws-an-open-label-study
#11
Francesco Calvani, Antimo Cutone, Maria Stefania Lepanto, Luigi Rosa, Valentino Valentini, Piera Valenti
Osteonecrosis of the jaws is an emerging pathological condition characterized by un-exposure or exposure of the necrotic bone, independently from the etiology. This term is usually referred to medication-related osteonecrosis of the jaws due to severe adverse reaction to certain medicines, as bisphosphonates, used for the treatment of cancer and osteoporosis. The management of patients with Bisphosphonate-Related Osteonecrosis of the Jaws (BRONJ) remains challenging because surgical and medical interventions may not eradicate this pathology...
February 12, 2018: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
https://www.readbyqxmd.com/read/29431233/lactoferrin-increases-sperm-membrane-functionality-of-frozen-equine-semen
#12
H S Martins, G C da Silva, S F Cortes, F O Paes, O A Martins Filho, Mss Araujo, R Stahlberg, M A Lagares
During cryopreservation, sperm was submitted to an increase in reactive oxygen species generation. This work aimed to improve the quality of frozen equine sperm after the addition of antioxidants lactoferrin (Lf) and catalase (Cat) to a freezing extender. Semen from six stallions was frozen with the extenders: F1) control, INRA 82 freezing extender, F2) F1 + 500 μg/ml Lf and F3) F1 + 200 IU/ml Cat. After thawing, sperm motility parameters, membrane functionality and integrity, and acrosome integrity and spontaneous acrosome-reacted sperm were evaluated with a computer-assisted sperm analysis, a hypoosmotic swelling test and epifluorescent microscopy, respectively...
February 12, 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29413114/variations-in-serum-concentrations-of-c-reactive-protein-ceruloplasmin-lactoferrin-and-myeloperoxidase-and-their-interactions-during-normal-human-pregnancy-and-postpartum-period
#13
Ewa Skarżyńska, Hanna Zborowska, Artur J Jakimiuk, Maria Karlińska, Barbara Lisowska-Myjak
BACKGROUND: Serum proteins may provide information about homeostasis of redox status and inflammatory processes also during pregnancy. The aim of the study was to assess the dynamics of changes in serum concentrations of C-reactive protein (CRP), ceruloplasmin (CP), lactoferrin (LF) and myeloperoxidase (MPO) and their interactions during normal pregnancy and the postpartum period. METHODS: The concentrations of proteins were measured in serum (n=113) from pregnant in consecutive trimesters and in postpartum period (n=28) and in non-pregnant women (n=17), using immunoturbidimetric assays (CRP, CP) and ELISA Kits (LF, MPO)...
March 2018: Journal of Trace Elements in Medicine and Biology
https://www.readbyqxmd.com/read/29410669/lactoferrin-containing-immunocomplexes-drive-the-conversion-of-human-macrophages-from-m2-into-m1-like-phenotype
#14
Chen-Hui Gao, Hong-Liang Dong, Li Tai, Xiao-Ming Gao
Macrophages are multifunctional cells that perform diverse roles in health and disease and considered the main source of inflammatory cytokines in affected joints of patients with rheumatoid arthritis (RA). M2 macrophages are well known as anti-inflammation and wound-healing cells; however, recent evidence suggests that they can also promote inflammation in RA, although the underlying mechanism remains to be clarified. Based upon our recent finding that lactoferrin (LTF)-containing IgG immunocomplex (LTF-IC), found elevated in RA sera, potent activators of human monocytes/macrophages, we herein demonstrate that LTF-IC was able to elicit immediate proinflammatory cytokine production by M2-polarized human macrophages through coligation with CD14/toll-like receptor (TLR) 4 and FcγRIIa (CD32a)...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29409862/the-antimicrobial-cathelicidin-peptide-hlf-1-11-attenuates-alveolar-macrophage-pyroptosis-induced-by-acinetobacter-baumannii-in-vivo
#15
Minhui Dai, Pinhua Pan, Haitao Li, Shuai Liu, Lemeng Zhang, Chao Song, Yi Li, Qian Li, Zhi Mao, Yuan Long, Xiaoli Su, Chengping Hu
Acinetobacter baumannii is a Gram-negative coccobacillus found primarily in hospital settings that has recently emerged as a source of hospital-acquired infections, including bacterial pneumonia. The hLF(1-11) peptide comprising the first 11 N-terminal residues of human lactoferrin exerts antimicrobial activity in vivo and was highly effective against multidrug-resistant A. baumannii strains in vitro and in vivo. Pyroptosis is a caspase-1-dependent inflammatory cell death process and is induced by various microbial infections...
February 1, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29409860/feeding-the-developing-brain-juvenile-rats-fed-diet-rich-in-prebiotics-and-bioactive-milk-fractions-exhibit-reduced-anxiety-related-behavior-and-modified-gene-expression-in-emotion-circuits
#16
Agnieszka Mika, Michelle Gaffney, Rachel Roller, Abigail Hills, Courtney A Bouchet, Kristina A Hulen, Robert S Thompson, Maciej Chichlowski, Brian M Berg, Monika Fleshner
Early life nutrition is critical for brain development. Dietary prebiotics and bioactive milk fractions support brain development by increasing plasticity and altering activity in brain regions important for cognition and emotion regulation, perhaps through the gut-microbiome-brain axis. Here we examined the impact of a diet containing prebiotics, lactoferrin, and milk fat globule membrane (test diet) on beneficial gut bacteria, basal gene expression for activity and plasticity markers within brain circuits important for cognition and anxiety, and anxiety-related behavior in the open field...
January 30, 2018: Neuroscience Letters
https://www.readbyqxmd.com/read/29397184/hot-topic-holder-pasteurization-of-human-milk-affects-some-bioactive-proteins
#17
André F Guerra, Caroline Mellinger-Silva, Amauri Rosenthal, Rosa H Luchese
The aim of this research was to investigate the effect of Holder pasteurization (HoP; 62.5°C, 30 min) on the protein profile and activities of glutathione peroxidase (GPx) and lysozyme (LZ) in human milk. Over 6 mo of lactation, human milk samples were analyzed before (raw) and after HoP for GPx and LZ activity and electrophoresis protein profile. Holder pasteurization reduced human milk lactoferrin, immunoglobulin fractions, and GPx activity. In addition, GPx activity, which is high in colostrum and transitional milk, was naturally reduced over the 6-mo lactation period...
January 31, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29394034/iron-and-its-role-in-cancer-defense-a-double-edged-sword
#18
Frank Thévenod
Iron (Fe) is an essential metal, vital for biological functions, including electron transport, DNA synthesis, detoxification, and erythropoiesis that all contribute to metabolism, cell growth, and proliferation. Interactions between Fe and O2 can result in the generation of reactive oxygen species (ROS), which is based on the ability of Fe to redox cycle. Excess Fe may cause oxidative damage with ensuing cell death, but DNA damage may also lead to permanent mutations. Hence Fe is carcinogenic and may initiate tumor formation and growth, and also nurture the tumor microenvironment and metastasis...
February 5, 2018: Metal Ions in Life Sciences
https://www.readbyqxmd.com/read/29393509/human-lactoferrin-attenuates-the-proinflammatory-response-of-neonatal-monocyte-derived-macrophages
#19
Lukas Wisgrill, Isabelle Wessely, Andreas Spittler, Elisabeth Förster-Waldl, Angelika Berger, Kambis Sadeghi
Bioactive components of human milk such as human lactoferrin (hLF) play an essential role in gut microbiome homeostasis and protection against neonatal inflammatory diseases. Neonatal intestinal macrophages display a pro-inflammatory profile that might contribute to inflammatory mucosal injury. Therefore, the aim of the study was to investigate the immunomodulatory effects of hLF on differentiation and activation of monocyte-derived macrophages (moMϕ). Monocytes isolated from umbilical cord blood of term neonates and peripheral blood of healthy adults were differentiated in the absence or presence of hLF and differentiation, apoptosis and phagocytosis were evaluated...
February 2, 2018: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/29393146/correlation-of-fecal-markers-with-magnifying-endoscopic-stratification-in-patients-with-ulcerative-colitis-who-are-in-clinical-remission
#20
Shoichiro Mine, Fuminao Takeshima, Yuko Akazawa, Kayoko Matsushima, Hitomi Minami, Naoyuki Yamaguchi, Ken Ohnita, Hajime Isomoto, Kazuhiko Nakao
BACKGROUND: Maintenance of mucosal healing is recommended during the treatment of ulcerative colitis (UC). However, symptoms of UC often do not reflect mucosal disease activity. Fecal markers such as calprotectin, lactoferrin, and hemoglobin have been reported to correlate well with the Mayo endoscopic subscore (MES) and are being considered alternative monitoring tools in endoscopy. Ulcerative Colitis Endoscopic Index of Severity (UCEIS) is a new and more detailed endoscopic scoring system compared to MES...
February 1, 2018: Digestion
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