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Barbara Lisowska-Myjak, Ewa Skarżyńska, Maria Płazińska, Artur Jakimiuk
Meconium concentrations of naturally accumulated ceruloplasmin (CER), lactoferrin (LF), and neutrophil gelatinase-associated lipocalin (NGAL) and their relationships considered as a panel of acute phase proteins could be used for the assessment of fetal homeostasis. CER, LF and NGAL concentrations were measured using ELISA kits in meconium portions (n=80) collected from 19 healthy neonates. The coefficients of variation (CV) of the meconium LF (1.77) and NGAL (1.26) were about 2-fold higher than that of CER (0...
June 16, 2018: Clinical and Experimental Pharmacology & Physiology
Yani Dai, Xuezhen Yang, Shen Liu, Jianwu Wang, Qixian Zhou, Rui Fang, Tian Yu
OBJECTIVE: To study the edible safety of recombinant human lactoferrin( rh LF) expressed from transgenic cow mammary gland bioreactor. METHODS: According to the food additive safety toxicology evaluation procedures and method, acute oral toxicity in rats and 90 day subchronic toxicity test in mice were done to evaluate the edible safety of rh LF. RESULTS: Acute oral toxicity test indicated that rh LF was no toxic effect during the observation period, mouse acute oral LD50 of recombinant human lactoferrin was greater than 20 000 mg/kg...
March 2018: Wei Sheng Yan Jiu, Journal of Hygiene Research
Sebastian Ganz, Michael Bülte, Zdzislaw Gajewski, Axel Wehrend
The best studied substances in bovine colostrum are the immunoglobulins. They are absorbed in the small intestine of the neonate by pinocytosis. The Fc-receptor is not highly involved in this process in calves compared to other species. However, this receptor plays a crucial role in the transport of immunoglobulins from the circulation of the dam to the udder and, therefore, into the colostrum. During colostrogenesis, which starts up to 8 weeks prior to parturition, up to 500 g of immunoglobulins are transferred daily by this process...
June 2018: Tierärztliche Praxis. Ausgabe G, Grosstiere/Nutztiere
Karin Pichler, Benjamin Bausenhardt, Steliana Huhulescu, Claudia Lindtner, Alexander Indra, Franz Allerberger, Angelika Berger
BACKGROUND: Clostridium difficile is a gram-positive, anaerobic spore-forming, toxin-producing bacillus, which is one of the most common causes for health care-associated infections. High colonization rates in clinically asymptomatic neonates and infants have been described, although most studies go back to the early 1980 and 1990s, and were carried out in term and late preterm infants. OBJECTIVES: The aim of our study was to determine both the impact and time course of C...
June 12, 2018: Neonatology
Dario Sorrentino, Vu Q Nguyen
Background: In Crohn's disease (CD) a small bowel study-in addition to colonoscopy-is considered necessary for diagnosis/staging. In this study we re-examined the role of capsule endoscopy (CE), colonoscopy, imaging tests [magnetic resonance enterography/computed tomographic enterography (MRE/CTE)], and inflammatory markers [fecal lactoferrin and C-reactive protein (FL/CRP)] in CD patients who had undergone intestinal resection and in those who never had surgery. Methods: In this retrospective study 43 consecutive patients underwent CE because of staging/symptoms unexplained by colonoscopy/imaging...
June 8, 2018: Inflammatory Bowel Diseases
(no author information available yet)
In a multi-centre randomised controlled trial (RCT), we are assessing whether giving very preterm (i.e., born at < 32 weeks' gestation) infants prophylactic enteral bovine lactoferrin supplementation (150 mg/kg/day) from shortly after birth until 34 weeks' post-menstrual age reduces the incidence of late-onset invasive infection (primary outcome), all-cause mortality, bronchopulmonary dysplasia, necrotising enterocolitis, retinopathy of prematurity, and the duration of antibiotic exposure, intensive care, and hospital admission...
June 11, 2018: Neonatology
Abass Tanhaiean, Marjan Azghandi, Jamshid Razmyar, Elyass Mohammadi, Mohammad Hadi Sekhavati
Over the last decades, poultry industry faced to the rapid emergence of multidrug-resistant bacteria as a global concern. Antimicrobial peptide (AMPs) known as potential antibiotic alternative and were considered as a new antimicrobial agent. Current methods of production and purification of AMPs have several limitations such as: costly, time-consuming and killing the producing host cells in recombinant form. In the present study, a chimeric peptide derived from camel lactoferrin was produced in Escherichia coli periplasmic space using a pET-based expression system and its antibacterial activity was determined on some avian pathogens in vitro...
June 8, 2018: Microbial Pathogenesis
C-H Lee, Y-W Chen, Y-K Tu, Y-C Wu, P-C Chang
BACKGROUND AND OBJECTIVE: The response to nonsurgical periodontal treatment varied among patients. This study assessed the potential of salivary biomarkers for predicting the sensitivity and monitoring the response to nonsurgical periodontal therapy. MATERIAL AND METHODS: This study recruited 34 participants with severe chronic periodontitis (the test group) and 20 participants without periodontal destruction in any teeth (the control group) from September 6, 2013 to August 25, 2017...
June 7, 2018: Journal of Periodontal Research
Atef Darwish
No abstract text is available yet for this article.
June 7, 2018: Journal of Maternal-fetal & Neonatal Medicine
E Zaczyńska, I Kochanowska, M Kruzel, M Zimecki
Immature B cells are susceptible to apoptosis due to ligation of surface immunoglobulin receptors. The WEHI 231 cell line represents a useful model to study the mode of action of factors preventing apoptosis. In this work we investigated the protective effects of multi-species lactoferrins in anti-mouse Ig-induced WEHI 231 cell death. Bovine milk-derived lactoferrin (bLF), recombinant human lactoferrin expressed in Chinese hamster ovary cells - rhLF(CHO) or in human endothelial kidney cells - rhLF(HEK), and recombinant mouse lactoferrin expressed in Chinese hamster ovary cells - rmLF(CHO), were used...
2018: Folia Biologica (Praha)
Raymond Farah, Hayat Haraty, Ziad Salame, Youssef Fares, David M Ojcius, Najwane Said Sadier
Current research efforts on neurological diseases are focused on identifying novel disease biomarkers to aid in diagnosis, provide accurate prognostic information and monitor disease progression. With advances in detection and quantification methods in genomics, proteomics and metabolomics, saliva has emerged as a good source of samples for detection of disease biomarkers. Obtaining a sample of saliva offers multiple advantages over the currently tested biological fluids as it is a non-invasive, painless and simple procedure that does not require expert training or harbour undesirable side effects for the patients...
April 2018: Biomedical Journal
R J Carman, H M Daskalovitz, M W Lyerly, M Y Davis, M V Goodykoontz, J H Boone
The excess from fecal samples submitted to a centralized laboratory in Roanoke, Virginia for routine C. difficile testing was used for this research study. We tested all samples, including any formed samples usually not assayed in diagnostic laboratories. Our first aim was to rank ribotypes by their frequency. Between 2007 and 2013, fluoroquinolone resistant 027 (027FQR), a multi-drug resistant ribotype, was 32% of 3118 Clostridium difficile isolates and the most common of 128 ribotypes. 027FQR was in 45% of cytotoxin positive but only 17% of cytotoxin negative fecal samples (p = 0...
May 17, 2018: Anaerobe
Jicai Zhang, Xiang Xiao, Jianming Zhu, Ziyun Gao, Xianliang Lai, Xingen Zhu, Guohua Mao
Purpose: Glioblastoma multiforme (GBM) is the most common malignant brain tumor originating in the central nervous system in adults. Based on nanotechnology such as liposomes, polymeric nanoparticles, and lipid nanoparticles, recent research efforts have been aimed to target drugs to the brain. Methods: In this study, lactoferrin- and arginine-glycine-aspartic acid (RGD) dual- ligand-comodified, temozolomide and vincristine-coloaded nanostructured lipid carriers (L/RT/V-NLCs) were introduced for GBM combination therapy...
2018: International Journal of Nanomedicine
Juan C Nieto, Lídia Perea, Germán Soriano, Carlos Zamora, Elisabet Cantó, Aina Medina, Maria Poca, Elisabet Sanchez, Eva Roman, Germà Julià, Ferran Navarro, Cristina Gely, Edilmar A Alvarado, Carlos Guarner, Cándido Juarez, Sílvia Vidal
Ascitic neutrophils from cirrhotic patients with spontaneous bacterial peritonitis (SBP) exhibit an impaired oxidative burst that could facilitate bacterial infection. However, the influence of the cell-free ascitic fluid of these patients on neutrophil function has not been investigated. To analyze this influence, we determined the ascitic levels of cytokines, resistin, and lactoferrin and their association with neutrophil function, disease severity score, and SBP resolution. We analyzed NETosis induction by microscopy and oxidative burst by the flow cytometry of healthy neutrophils cultured in ascitic fluid from cirrhotic patients with sterile ascites (SA) and with SBP before and after antibiotic treatment...
May 31, 2018: Journal of Leukocyte Biology
Tokiko Shinjo, Keishoku Sakuraba, Atsuko Nakaniida, Tomoyo Ishibashi, Miki Kobayashi, Yuya Aono, Yoshio Suzuki
Lactoferrin is a secretory protein with various physiological functions. Bovine lactoferrin has been demonstrated to alleviate psychological stresses in rats, but this effect in humans has not yet been assessed. The present study aimed to investigate the changes in psychological stress markers following a calculation task, with either lactoferrin or a placebo orally administered prior to the task. A total of 16 healthy female college students visited Juntendo University, Inzai, Japan following an overnight fast...
May 2018: Biomedical Reports
Reena Khanna, Neeraj Narula, Brian G Feagan
Clinical trials require valid outcome measures to assess the therapeutic benefit of investigational agents. Recently, regulatory authorities have mandated the use of patient-reported outcomes in combination with an objective measure of disease activity as primary outcome measures in inflammatory bowel disease trials. Endoscopy has commonly fulfilled the latter role; however, due to the costs and complexity of these assessments, interest has emerged in the use of noninvasive biomarkers. The role of C-reactive protein, fecal calprotectin, and fecal lactoferrin in clinical research is discussed...
May 28, 2018: Inflammatory Bowel Diseases
Andrea Galassi, Liliana Turatello, Alessandra De Salvia, Margherita Neri, Emanuela Turillazzi, Raffaele La Russa, Rocco V Viola, Paola Frati, Vittorio Fineschi
Current scientific consensus about the physiopathology in the progression from severe sepsis to septic shock and death focuses on myocardial contractile dysfunction. Nevertheless, objective parameters to establish a pathological correlate of a fatal outcome are lacking; then a cause of death due to sepsis can remain an unsolved problem. We first reviewed all death cases recorded at our institutions during the period from 2007 until 2015. Then, we conducted a retrospective study of a selected autopsy series of people who had received "sepsis" as cause of death...
January 2018: International Journal of Immunopathology and Pharmacology
Salma N Tammam, Hassan M E Azzazy, Alf Lamprecht
Lactoferrin (Lf) exerts anti-cancer effects on glioma, however, the exact mechanism remains unclear. Despite possessing a nuclear localization sequence (NLS), Lf was found to allocate only in the cytoplasm of glioma 261. Lf was therefore loaded into nuclear and cytoplasmic targeted nanoparticles (NPs) to determine whether nuclear delivery of Lf would enhance its anti-cancer effect. Upon treatment with 300 and 800 µg/mL Lf loaded chitosan NPs, nuclear targeted Lf-NPs showed 1.3 and 2.7 folds increase in cell viability, whereas cytoplasmic targeted Lf-NPs at 300 µg/mL decreased cell viability by 0...
May 23, 2018: European Journal of Pharmaceutics and Biopharmaceutics
Asim Halder, Divanshu Shukla, Suvadra Das, Partha Roy, Arup Mukherjee, Bhaskar Saha
Visceral Leishmaniasis (VL), caused by the protozoan parasite Leishmania donovani, is a potentially fatal disease. The only orally bioavailable drug miltefosine is toxic and the effective liposomal Amphotericin B (AmBisome) is limited by its prohibitive cost and requirement for parenteral administration. Therefore, finding a new potential drug candidate and an alternative delivery system is imperative. We report that Betulinic acid (BA), a pentacyclic triterpenoid from Betula alba bark, was loaded onto uniformly spherical PLGA nanoparticles (BANPs; diameter 187...
May 18, 2018: Cytokine
Hans J Vogel, Antimo Cutone, Maria S Lepanto, Luigi Rosa, Piera Valenti
No abstract text is available yet for this article.
June 2018: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
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