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https://www.readbyqxmd.com/read/28935851/comparative-analysis-of-the-microbial-communities-in-raw-milk-produced-in-different-regions-of-korea
#1
In Seon Kim, Yoo Kyung Hur, Eun Ji Kim, Young-Tae Ahn, Jong Geun Kim, Yun-Jaie Choi, Chul Sung Huh
Objective: The control of psychrotrophic bacteria bringing about the milk spoilage and the illness by toxic compounds is an important issue in the dairy industry. In South Korea, Gangwon-do province is one of the coldest mountain terrain in which eighty percent of the areas are mountainous regions, and mainly play an important role in agriculture and dairy industry. The purposes of this study were to analyze the indigenous microbiota of raw milk in Gangwon-do and accurately investigate a putative microbial group causing milk quality to be deteriorated...
September 21, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28935254/design-of-gefitinib-loaded-poly-l-lactic-acid-microspheres-via-a-supercritical-anti-solvent-process-for-dry-powder-inhalation
#2
Qing Lin, Guijin Liu, Ziyi Zhao, Dongwei Wei, Jiafeng Pang, Yanbin Jiang
To develop a safer, more stable and potent formulation of gefitinib (GFB), micro-spheres of GFB encapsulated into poly (L-lactic acid) (PLLA) have been prepared by supercritical anti-solvent (SAS) technology in this study. Operating factors were optimized using a selected OA16 (4(5)) orthogonal array design, and the properties of the raw material and SAS processed samples were characterized by different methods The results show that the GFB-loaded PLLA particles prepared were spherical, having a smaller and narrower particle size compared with raw GFB...
September 18, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28934853/responsive-photonic-crystal-carbohydrate-hydrogel-sensor-materials-for-selective-and-sensitive-lectin-protein-detection
#3
Zhongyu Cai, Aniruddha Sasmal, Xinyu Liu, Sanford A Asher
Lectin proteins, such as the highly toxic lectin protein, ricin, and the immunochemically important lectin, jacalin, play significant roles in many biological functions. It is highly desirable to develop a simple but efficient method to selectively detect lectin proteins. Here we report the development of carbohydrate containing responsive hydrogel sensing materials for the selective detection of lectin proteins. The copolymerization of a vinyl linked carbohydrate monomer with acrylamide and acrylic acid forms a carbohydrate hydrogel that shows specific "multivalent" binding to lectin proteins...
September 22, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28934590/the-relationship-between-the-human-genome-and-microbiome-comes-into-view
#4
Julia K Goodrich, Emily R Davenport, Andrew G Clark, Ruth E Ley
The body's microbiome, composed of microbial cells that number in the trillions, is involved in human health and disease in ways that are just starting to emerge. The microbiome is assembled at birth, develops with its host, and is greatly influenced by environmental factors such as diet and other exposures. Recently, a role for human genetic variation has emerged as also influential in accounting for interpersonal differences in microbiomes. Thus, human genes may influence health directly or by promoting a beneficial microbiome...
September 20, 2017: Annual Review of Genetics
https://www.readbyqxmd.com/read/28925965/studying-the-structural-significance-of-galectin-design-by-playing-a-modular-puzzle-homodimer-generation-from-human-tandem-repeat-type-heterodimeric-galectin-8-by-domain-shuffling
#5
Anna-Kristin Ludwig, Malwina Michalak, Nadya Shilova, Sabine André, Herbert Kaltner, Nicolai V Bovin, Jürgen Kopitz, Hans-Joachim Gabius
Tissue lectins are emerging (patho)physiological effectors with broad significance. The capacity of adhesion/growth-regulatory galectins to form functional complexes with distinct cellular glycoconjugates is based on molecular selection of matching partners. Engineering of variants by changing the topological display of carbohydrate recognition domains (CRDs) provides tools to understand the inherent specificity of the functional pairing. We here illustrate its practical implementation in the case of human tandem-repeat-type galectin-8 (Gal-8)...
September 19, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28922262/sugar-in-infants-children-and-adolescents-a-position-paper-of-the-european-society-for-paediatric-gastroenterology-hepatology-and-nutrition-committee-on-nutrition
#6
Nataša Fidler Mis, Christian Braegger, Jiri Bronsky, Cristina Campoy, Magnus Domellöf, Nicholas D Embleton, Iva Hojsak, Jessie Hulst, Flavia Indrio, Alexandre Lapillonne, Walter Mihatsch, Christian Molgaard, Rakesh Vora, Mary Fewtrell
The consumption of sugars, particularly sugar-sweetened beverages (SSBs; beverages or drinks that contain added caloric sweeteners (i.e. sucrose, high-fructose corn syrup, fruit-juice concentrates), in European children and adolescents exceeds current recommendations. This is of concern because there is no nutritional requirement for free sugars, and infants have an innate preference for sweet taste, which may be modified and reinforced by pre- and postnatal exposures. Sugar containing beverages/free sugars increase the risk for overweight/obesity and dental caries, can result in poor nutrient supply and reduced dietary diversity and may be associated with increased risk of type 2 diabetes mellitus, cardiovascular risk, and other health effects...
September 16, 2017: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/28922259/gastrointestinal-symptoms-of-food-challenge-proven-non-ige-cow-s-milk-allergy-are-dissipated-by-early-school-age
#7
Noora Helin, Kaija-Leena Kolho, Erik Qvist, Mika Mäkelä, Laura Merras-Salmio
OBJECTIVES: To evaluate the current well-being and dietary restrictions in children six years after food challenge confirmed diagnosis of non-IgE cow's milk protein allergy, compared to peers with gastrointestinal symptoms but negative food challenge. To evaluate the diagnostic process retrospectively. METHODS: Internet-based survey for mothers whose children underwent six years ago the double-blind, placebo-controlled food challenge for cow's milk because of gastrointestinal symptoms causing suspicion of non-IgE cow's milk protein allergy...
September 16, 2017: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/28920406/evaluation-of-high-nutrient-diets-on-litter-performance-of-heat-stressed-lactating-sows
#8
Yohan Choi, Abdolreza Hosseindoust, Minju Kim, Alip Kumar, Seungmin Oh, Young Hwa Kim, Byung Jo Chae
Objective: The present study investigated the litter performance of multiparous sows fed 3% and 6% densified diets at farrowing to weaning during summer with mean maximum room temperature of 30.5°C. Methods: A total of 60 crossbred multiparous sows were allotted to one of three treatments based on body weight according to a completely randomized design. Three different nutrient levels based on NRC were applied as standard diet (ST; ME, 3300 kcal/kg), high nutrient level 1 (HE1; ST + 3% higher energy and 16...
September 18, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28919679/effects-of-intramammary-infusion-of-sage-salvia-officinalis-essential-oil-on-milk-somatic-cell-count-milk-composition-parameters-and-selected-hematology-and-serum-biochemical-parameters-in-awassi-sheep-with-subclinical-mastitis
#9
Myassar O Alekish, Zuhair B Ismail, Mofleh S Awawdeh, Shoroq Shatnawi
AIM: The aims of this study were to evaluate the effects of intramammary infusion of sage (Salvia officinalis) essential oil (EO) on milk somatic cell count (SCC), milk composition parameters and selected hematology and serum biochemical parameters in 20 Awassi ewes affected with subclinical mastitis. MATERIALS AND METHODS: The dried leaves of sage were used to extract the EO by hydrodistillation. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of sage EO against Staphylococcus aureus were determined by the broth dilution method...
August 2017: Veterinary World
https://www.readbyqxmd.com/read/28918149/predicting-methane-emissions-of-lactating-danish-holstein-cows-using-fourier-transform-mid-infrared-spectroscopy-of-milk
#10
N Shetty, G Difford, J Lassen, P Løvendahl, A J Buitenhuis
Enteric methane (CH4), a potent greenhouse gas, is among the main targets of mitigation practices for the dairy industry. A measurement technique that is rapid, inexpensive, easy to use, and applicable at the population level is desired to estimate CH4 emission from dairy cows. In the present study, feasibility of milk Fourier transform mid-infrared (FT-IR) spectral profiles as a predictor for CH4:CO2 ratio and CH4 production (L/d) is explained. The partial least squares regression method was used to develop the prediction models...
September 13, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28918133/short-communication-short-term-intravenous-amino-acid-infusions-as-a-method-to-detect-limiting-amino-acids-in-dairy-cattle-diets
#11
Roni Yair, Michael S Allen
We hypothesized that the addition of limiting AA increases dry matter intake (DMI) by reducing anaplerosis and hepatic oxidation. Accordingly, the objective of this work was to examine the effects of short-term intravenous infusions of Met, Lys, and His (which are considered the most limiting AA) on DMI as a method to detect whether specific AA are limiting in dairy cow diets. We conducted 4 experiments using Holstein cows in the immediate postpartum period to address this objective. The first experiment used 4 cows 6 to 10 d postpartum (PP) in a 4 × 4 Latin square design with 1-d periods including 12 h for infusions and 12 h for recovery...
September 13, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28917905/characterization-of-a-water-soluble-chitosan-derivative-and-its-potential-for-submucosal-injection-in-endoscopic-techniques
#12
Hidemi Hattori, Hironori Tsujimoto, Kazuo Hase, Masayuki Ishihara
To examine the potential of chitosan-based agents for submucosal injection in endoscopic techniques, a chitosan derivative was prepared with lactose moieties linked to the amino groups of its glucosamine units (CH-LA). After dissolving CH-LA in neutral pH solutions, including physiological saline (CH-LA-S), its response to different concentrations of anionic glycosaminoglycans and proteins in the surrounding environment was examined. The CH-LA-S form changed in the presence of sulfated glycosaminoglycans (heparin, chondroitin sulfate, and mucin) and protein (fibrinogen)...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28917009/preparation-characterization-and-in-vitro%C3%A2-%C3%A2-in-vivo-evaluation-of-oral-time-controlled-release-etodolac-pellets
#13
Xiaoyu Zhang, Qi Li, Mingzhu Ye, Zhinan Zhao, Jiayi Sun, Xinggang Yang, Weisan Pan
The objective of this study was to prepare time-controlled release etodolac pellets to facilitate drug administration according to the body's biological rhythm, optimize the drug's desired effects, and minimize adverse effects. The preparation consisted of three laminal layers from center to outside: the core, the swelling layer, and the insoluble polymer membrane. Factors influenced the core and the coating films were investigated in this study. The core pellets formulated with etodolac, lactose, and sodium carboxymethyl starch (CMS-Na) were prepared by extrusion-spheronization and then coated by a fluidized bed coater...
September 15, 2017: AAPS PharmSciTech
https://www.readbyqxmd.com/read/28911656/physicochemical-analysis-and-nonisothermal-kinetic-study-of-sertraline-lactose-binary-mixtures
#14
Faranak Ghaderi, Mahboob Nemati, Mohammad Reza Siahi-Shadbad, Hadi Valizadeh, Farnaz Monajjemzadeh
In the present study the physicochemical stability of sertraline with lactose was evaluated in drug-excipient binary mixtures. Different physicochemical methods such as differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy, and mass spectrometry were applied to confirm the incompatibility. The final aim of this study was to evaluate the kinetic parameters using a fast and sensitive DSC method. Solid-state kinetic parameters were derived from nonisothermally stressed physical mixtures using different thermal models such as Friedman, Flynn-Wall-Ozawa, and Kissinger-Akahira-Sunose...
July 2017: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28911188/diversity-of-ochrobactrum-species-in-food-animals-antibiotic-resistance-phenotypes-and-polymorphisms-in-the-blaoch-gene
#15
Carla Andrea Alonso, Yakubu A Kwabugge, Madubuike U Anyanwu, Carmen Torres, Kennedy F Chah
Twenty-six lactose non-fermenting, oxidase, urease and citrate-positive Gram-negative rods, isolated from broiler chickens, pigs and cattle at slaughter, were subjected to the matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry and 16S rDNA sequencing for identification. Susceptibility to 14 antimicrobials was determined by the disc diffusion method. Ochrobactrum isolates resistant to third-generation cephalosporins were PCR-screened for the presence of the Ochrobactrum anthropi ampC gene (blaOCH)...
September 15, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28905280/enzymatic-synthesis-of-fucose-containing-galacto-oligosaccharides-using-%C3%AE-galactosidase-and-identification-of-novel-disaccharide-structures
#16
Anne Usvalampi, Hannu Maaheimo, Olli Tossavainen, Alexander D Frey
Fucosylated oligosaccharides have an important role in maintaining a healthy immune system and homeostatic gut microflora. This study employed a commercial β-galactosidase in the production of fucose-containing galacto-oligosaccharides (fGOS) from lactose and fucose. The production was optimized using experiment design and optimal conditions for a batch production in 3-liter scale. The reaction product was analyzed and the produced galactose-fucose disaccharides were purified. The structures of these disaccharides were determined using NMR and it was verified that one major product with the structure Galβ1-3Fuc and two minor products with the structures Galβ1-4Fuc and Galβ1-2Fuc were formed...
September 13, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28905245/identification-of-polymorphic-forms-of-active-pharmaceutical-ingredient-in-low-concentration-dry-powder-formulations-by-synchrotron-x-ray-powder-diffraction
#17
Kenji Egusa, Fumiaki Okazaki, Joerg Schiewe, Ulrike Werthmann, Markus Wolkenhauer
BACKGROUND: The identification of different (pseudo) polymorphs of an active pharmaceutical ingredient in dry powder formulations is of importance during development and entire product lifecycle, e.g., quality control. Whereas determination of polymorphic differences of pure substances is rather easy, in dry powder formulations, it is generally difficult and the difficulties increase particularly, if the substance of interest is present only in low concentrations in the formulation. Such a formulation is SpirivaR inhalation powder (Boehringer Ingelheim), which contains only 0...
September 13, 2017: Drugs in R&D
https://www.readbyqxmd.com/read/28900755/quantitative-physiology-and-elemental-composition-of-kluyveromyces-lactis-cbs-2359-during-growth-on-glucose-at-different-specific-growth-rates
#18
Oscar Dias, Thiago O Basso, Isabel Rocha, Eugénio C Ferreira, Andreas K Gombert
The yeast Kluyveromyces lactis has received attention both from academia and industry due to some important features, such as its capacity to grow in lactose-based media, its safe status, its suitability for large-scale cultivation and for heterologous protein synthesis. It has also been considered as a model organism for genomics and metabolic regulation. Despite this, very few studies were carried out hitherto under strictly controlled conditions, such as those found in a chemostat. Here we report a set of quantitative physiological data generated during chemostat cultivations with the K...
September 12, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28898738/different-magnesium-release-profiles-from-w-o-w-emulsions-based-on-crystallized-oils
#19
Sameh Herzi, Wafa Essafi
Water-in-oil-in-water (W/O/W) double emulsions based on crystallized oils were prepared and the release kinetics of magnesium ions from the internal to the external aqueous phase was investigated at T=4°C, for different crystallized lipophilic matrices. All the emulsions were formulated using the same surface-active species, namely polyglycerol polyricinoleate (oil-soluble) and sodium caseinate (water-soluble). The external aqueous phase was a lactose or glucose solution at approximately the same osmotic pressure as that of the inner droplets, in order to avoid osmotic water transfer phenomena...
August 31, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28890764/lactococcus-lactis-strain-a5-producing-nisin-like-bacteriocin-active-against-gram-positive-and-negative-bacteria
#20
Nur Shazana Azhar, Noor Hasniza Md Zin, Tengku Haziyamin Tengku Abdul Hamid
In this study, a Lactic acid bacteria (LAB) strain was isolated on MRS medium from gastro-intestinal tissues of Broadhead catfish (Clarias macrocephalus). Out of 50 isolates, 25 isolates were found to be positive on lactose utilisation test and were identified to be gram positive cocci. Using disc diffusion methods, one out of 22 isolates, i.e., a strain A5 demonstrated inhibitions against three indicator organisms; Bacillus cereus, Staphylococcus aureus and Salmonella thyphimurium. Partial 16S rDNA sequencing identified isolate A5 as a member of Lactococcus lactis, with 100% DNA homology...
July 2017: Tropical Life Sciences Research
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