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https://www.readbyqxmd.com/read/29356014/impact-of-carbohydrates-on-autoinducer-2-secretion-of-bifidobacterium-longum-subsp-longum-bbmn68
#1
Jingyi Song, Qian Qin, Tong Li, Fazheng Ren, Hongxing Zhang, Yuanhong Xie, Junhua Jin
In this study, the regularity of autoinducer-2 (AI-2) secretion during growth and the effect of the addition of various carbohydrates on AI-2 secretion in Bifidobacterium longum subsp. longum BBMN68 were investigated. The results indicated that the AI-2 concentration reached its highest level (2536.60 nmol l-1 ) at the early stationary growth phase, and then decreased to 1263.72 nmol l-1 at the late stationary growth phase in Bifidobacterium cultures. When the density of the cultures which mannose, fructose, sucrose, and lactose had been added to reached an OD600 nm of 1...
January 22, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29353279/lactobacillus-rhamnosus-gg-in-experimental-oral-biofilms-exposed-to-different-carbohydrate-sources
#2
Qingru Jiang, Veera Kainulainen, Iva Stamatova, Riitta Korpela, Jukka H Meurman
Probiotic administration may favour caries prevention, as recent research has shown. This in vitro study aimed to investigate the growth of Lactobacillus rhamnosus GG (LGG) in experimental biofilms exposed to various carbohydrates, and also to assess its cariogenic potential. Multispecies experimental oral biofilms with or without LGG were grown with a sole-carbohydrate source (fructose/glucose/lactose/sorbitol/sucrose). The viable cells of LGG and structure of the biofilms were examined after 64.5 h of incubation, and pH values of spent media were measured at 16...
January 20, 2018: Caries Research
https://www.readbyqxmd.com/read/29352487/disaccharide-combinations-and-the-expression-of-enolase3-and-plasma-membrane-ca2-atpase-isoform-in-sturgeon-sperm-cryopreservation
#3
M D Xi, P Li, H Du, X M Qiao, Z G Liu, Q W Wei
Acipenser sinensis and Acipenser dabryanus are critically endangered species, so germplasm conservation via cryopreservation of sperm is necessary. Disaccharides can act as membrane-impermeable cryoprotectants, and enolase3 (ENO3) and plasma membrane Ca2+ ATPase isoform (PMCA2) are proteins associated with sperm quality. We considered seven characteristics of sperm quality in cultured brood stock from A. sinensis and A. dabryanus. We tested use of sucrose or trehalose alone and in combination at different concentrations for cryopreservation of A...
January 19, 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29348099/spray-dried-curcumin-nanoemulsion-a-new-road-to-improvement-of-oral-bioavailability-of-curcumin
#4
Liandong Hu, Qiaofeng Hu, Chenchen Yang
In this study a new soluble solid curcumin nanoemulsion powder was prepared using spray-drying technology to improve the solubility and bioavailability of curcumin. The liquid nanoemulsion consisted of curcumin, Capryol 90, Transcutol P, and Cremophor RH40. The solid nanoemulsion was prepared by spray-drying the liquid nanoemulsion in laboratory spray dryer, using lactose as solid carrier. The in vitro release from powder formulation was 97.6% within 15 min while the release from the curcumin crystalline was about 10%...
January 2018: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29344229/preparation-and-characterization-of-the-adriamycin-loaded-amphiphilic-chitosan-nanoparticles-and-their-application-in-the-treatment-of-liver-cancer
#5
Chang-Hua Kou, Jin Han, Xi-Lin Han, Hui-Jie Zhuang, Zi-Ming Zhao
In the present study, two nanoparticles including lactose myristoyl carboxymethyl chitosan (LMCC) and algal polysaccharide myristoyl carboxymethyl chitosan (AMCC), were obtained for hepatic-targeted Adriamycin (ADM) drug delivery systems. ADM was successfully loaded into the LMCC or AMCC nanoparticle by dialysis. The release function and liver targeting of the nanoparticles was explored, and it was revealed that ADM release from the nanoparticles was greatest at acidic pH 5.5. ADM-conjugated nanoparticles were readily taken up by HU7 human hepatocellular carcinoma cells, relative to HT22 mouse hippocampal neuron cells in vitro...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29342350/real-time-monitoring-of-chemical-changes-in-three-kinds-of-fermented-milk-products-during-fermentation-using-quantitative-difference-nmr-spectroscopy
#6
Yi Lu, Hiroto Ishikawa, Yeondae Kwon, Fangyu Hu, Takuya Miyakawa, Masaru Tanokura
Fermented milk products are rising in popularity throughout the world because of their health benefits, including improving digestion, normalizing the function of the immune system, and aiding in weight management. This study applies an in situ quantitative NMR method to monitor chemical changes in three kinds of fermented milk products, Bulgarian yogurt, Caspian Sea yogurt and kefir, during fermentation. As a result, the concentration changes in nine organic compounds, α/β-lactose, α/β-galactose, lactic acid, citrate, ethanol, lecithin, and creatine, were monitored in real time...
January 17, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29339290/biocatalytic-strategies-in-the-production-of-galacto-oligosaccharides-and-its-global-status
#7
REVIEW
Parmjit S Panesar, Rupinder Kaur, Ram S Singh, John F Kennedy
Galactooligosaccharides (GOSs) are the non-digestible carbohydrates that are composed of 3-10 or longer molecules of galactose and the terminal glucose molecule. These are considered as prebiotics owing to their various health benefits, and therefore is the major focus of research. These are generally synthesized by the catalytic activity of the glycoside hydrolases (GH) utilizing lactose as a substrate, which results in the mixture of GOSs differing in their degree of polymerization. Different microbial glycoside hydrolases have been used for the production...
January 12, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29338242/in-situ-biocatalytic-production-of-trehalose-with-auto-induction-expression-of-trehalose-synthase
#8
Xincheng Yan, Liying Zhu, Yadong Yu, Qing Xu, He Huang, Ling Jiang
We developed an in situ biocatalytic process that couples auto-induction expression of trehalose synthase (TreS) and whole-cell catalysis for trehalose production. With lactose as the auto-inducer, the activity of recombinant TreS in recombinant Escherichia coli was optimized through a visualization method, which resulted in a maximum value of 12033 ± 730 U/mL in pH-stat fed-batch fermentation mode. Meanwhile, the permeability of the auto-induced E. coli increased significantly, which makes it possible to be directly used as a whole-cell biocatalyst for trehalose production, whereby the by-product glucose can also act as an extra carbon source...
January 17, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29335877/development-of-cyclic-amp-receptor-protein-based-artificial-transcription-factor-for-intensifying-gene-expression
#9
Peng Zhao, Wenjuan Wang, Pingfang Tian
Vector-dependent gene overexpression typically relies on an efficient operon and sufficient RNA polymerases (RNAPs). The lac (lactose) operon is a paradigm of transcription control, and cyclic AMP receptor protein (CRP) is a global regulator capable of recruiting RNAPs. However, the gap between lac operon and CRP has not been well bridged. In this work, CRP was fused to lac repressor protein (lacI) to form an artificial transcription factor (ATF) with the expectation that when LacI acted on the lacO-positioned upstream of gene of interest, the LacI-tethered CRP would trap RNAPs and thus improve the expression of PuuC, an aldehyde dehydrogenase catalyzing 3-hydroxypropionaldehyde (3-HPA) to 3-hydroxypropionic acid (3-HP) in Klebsiella pneumoniae...
January 16, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29335514/monitoring-single-cell-gene-regulation-under-dynamically-controllable-conditions-with-integrated-microfluidics-and-software
#10
Matthias Kaiser, Florian Jug, Thomas Julou, Siddharth Deshpande, Thomas Pfohl, Olin K Silander, Gene Myers, Erik van Nimwegen
Much is still not understood about how gene regulatory interactions control cell fate decisions in single cells, in part due to the difficulty of directly observing gene regulatory processes in vivo. We introduce here a novel integrated setup consisting of a microfluidic chip and accompanying analysis software that enable long-term quantitative tracking of growth and gene expression in single cells. The dual-input Mother Machine (DIMM) chip enables controlled and continuous variation of external conditions, allowing direct observation of gene regulatory responses to changing conditions in single cells...
January 15, 2018: Nature Communications
https://www.readbyqxmd.com/read/29331465/genome-wide-association-study-for-milk-infrared-wavenumbers
#11
Qiuyu Wang, Henk Bovenhuis
Individual wavenumbers of the infrared (IR) spectra of bovine milk have been shown to be moderately to highly heritable. The objective of this study was to identify genomic regions associated with individual milk IR wavenumbers. This is expected to provide information about the genetic background of milk composition and give insight in the relation between IR wavenumbers and milk components. For this purpose, a genome-wide association study was performed for a selected set of 50 individual IR wavenumbers measured on 1,748 Dutch Holstein cows...
January 10, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29330542/regulation-of-metabolism-in-escherichia-coli-during-growth-on-mixtures-of-the-non-glucose-sugars-arabinose-lactose-and-xylose
#12
Ehab M Ammar, Xiaoyi Wang, Christopher V Rao
Catabolite repression refers to the process where the metabolism of one sugar represses the genes involved in metabolizing another sugar. While glucose provides the canonical example, many other sugars are also known to induce catabolite repression. However, less is known about the mechanism for catabolite repression by these non-glucose sugars. In this work, we investigated the mechanism of catabolite repression in the bacterium Escherichia coli during growth on lactose, L-arabinose, and D-xylose. The metabolism of these sugars is regulated in a hierarchical manner, where lactose is the preferred sugar, followed by L-arabinose, and then D-xylose...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29320574/acceleration-of-small-bowel-motility-after-oral-administration-of-dai-kenchu-to-tj-100-assessed-by-cine-magnetic-resonance-imaging
#13
Akitoshi Inoue, Akira Furukawa, Hiroshi Yamamoto, Shinichi Ohta, Nguyen Dai Hung Linh, Tulyeubai Syerikjan, Sachiko Kaida, Tsuyoshi Yamaguchi, Satoshi Murata, Toru Obata, Masaji Tani, Kiyoshi Murata
Dai-kenchu-to (TJ-100) is an herbal medicine used to shorten the duration of intestinal transit by accelerating intestinal movement. However, intestinal movement in itself has not been evaluated in healthy volunteers using radiography, fluoroscopy, and radioisotopes because of exposure to ionizing radiation. The purpose of this study was to evaluate the effect of TJ-100 on intestinal motility using cinematic magnetic resonance imaging (cine MRI) with a steady-state free precession sequence. Ten healthy male volunteers received 5 g of either TJ-100 or lactose without disclosure of the identity of the substance...
2018: PloS One
https://www.readbyqxmd.com/read/29318690/lactose-repressor-hinge-domain-independently-binds-dna
#14
Joseph S Xu, Madeleine N Hewitt, Jaskeerat S Gulati, Matthew A Cruz, Hongli Zhan, Shirley Liu, Kathleen S Matthews
The short 8-10 amino acid "hinge" sequence in lactose repressor (LacI), present in other LacI/GalR family members, links DNA and inducer-binding domains. Structural studies of full-length or truncated LacI-operator DNA complexes demonstrate insertion of the dimeric helical "hinge" structure at the center of the operator sequence. This association bends the DNA ∼40° and aligns flanking semi-symmetric DNA sites for optimal contact by the N-terminal helix-turn helix (HtH) sequences within each dimer. In contrast, the hinge region remains unfolded when bound to nonspecific DNA sequences...
January 10, 2018: Protein Science: a Publication of the Protein Society
https://www.readbyqxmd.com/read/29316991/is-dietary-macronutrient-composition-during-pregnancy-associated-with-offspring-birth-weight-an-observational-study
#15
Sukshma S Sharma, Darren C Greenwood, Nigel A B Simpson, Janet E Cade
There is lack of evidence on the differential impact of maternal macronutrient consumption: carbohydrates (CHO), fats and protein on birth weight. We investigated the association between maternal dietary macronutrient intakes and their sub-components such as saccharides and fatty acids and birth weight. This analyses included 1,196 women with singleton pregnancies who were part of the CAffeine and REproductive health study in Leeds, UK between 2003 and 2006. Women were interviewed in each trimester. Dietary information was collected twice using a 24-h dietary recall about 8-12 weeks and 13-27 weeks of gestation...
January 10, 2018: British Journal of Nutrition
https://www.readbyqxmd.com/read/29316174/protective-effect-of-conditioner-agents-on-hair-treated-with-oxidative-hair-dye
#16
Robson M da Gama, Simone A França-Stefoni, Tânia C Sá-Dias, Valcinir Bedin, André R Baby, Maria Valéria R Velasco
BACKGROUND: Hair coloring is broadly used by women and men either to change their natural hair color or to delay the onset of gray hair. Oxidative dyes may damage the hair, as chemical and physical processes are required to convert the fiber structure and, consequently, alterations in its mechanical and surface properties. OBJECTIVES: The aim of this study was to evaluate the protective effect of silanetriol (and) Panthenol, PEG-12 dimethicone, and hydrolyzed silk (and) hydrolyzed milk protein (and) lactose as conditioner agents on hair treated with oxidative hair dye by protein loss, combability, and breaking strength...
January 7, 2018: Journal of Cosmetic Dermatology
https://www.readbyqxmd.com/read/29313426/enhancement-of-prodigiosin-synthetase-pigc-production-from-recombinant-escherichia-coli-through-optimization-of-induction-strategy-and-media
#17
Zhongyu You, Suping Zhang, Xiaoxia Liu, Yujie Wang
PigC is a synthetase that catalyzes the condensation of 4-methoxy-2,2'-bipyrrole-5-carboxyaldehyde (MBC) and 2-methyl-3-amylpyrrole (MAP) to produce prodigiosin, which has a wide variety of impressive biological properties. In this study, we optimized PigC production from engineered Escherichia coli BL21(DE3). Investigation of different induction strategies revealed that auto-induction was the most appropriate method for PigC expression. As a result, PigC activity was elevated to 75.7 U/mL, nearly 2.1-fold higher than that with induction by isopropy-β-D-thiogalactoside (IPTG)...
January 9, 2018: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/29311037/relationships-among-dietary-intakes-and-persistent-gastrointestinal-symptoms-in-patients-receiving-enzyme-treatment-for-genetic-sucrase-isomaltase-deficiency
#18
Anne Boney, Heather E Elser, Heidi J Silver
BACKGROUND: Sucrose-isomaltase deficiency (SID) remains underdiagnosed. Absent or reduced enzyme activity promotes diarrhea, abdominal bloating, and flatulence from undigested and malabsorbed disaccharides. Frequency and severity of gastrointestinal symptoms may be associated with the type of carbohydrates consumed. OBJECTIVE: To characterize the dietary intakes of patients treated with sacrosidase (Sucraid; QOL Medical) for SID and determine relationships between type of carbohydrates, sacrosidase dose, and gastrointestinal symptoms...
January 5, 2018: Journal of the Academy of Nutrition and Dietetics
https://www.readbyqxmd.com/read/29305981/the-effect-of-formulation-additives-on-in-vitro-dissolution-absorption-profile-and-in-vivo-bioavailability-of-telmisartan-from-brand-and-generic-formulations
#19
Enikő Borbás, Zsombor K Nagy, Brigitta Nagy, Attila Balogh, Balázs Farkas, Oksana Tsinman, Konstantin Tsinman, Bálint Sinkó
In this study, brand and four generic formulations of telmisartan, an antihypertensive drug, were used in in vitro simultaneous dissolution-absorption, investigating the effect of different formulation additives on dissolution and on absorption through an artificial membrane. The in vitro test was found to be sensitive enough to show even small differences between brand and generic formulations caused by the use of different excipients. By only changing the type of filler from sorbitol to mannitol in the formulation, the flux through the membrane was reduced by approximately 10%...
January 3, 2018: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29303609/buffering-agent-induced-lactose-content-increases-via-growth-hormone-mediated-activation-of-gluconeogenesis-in-lactating-goats
#20
L Li, M L He, Y Liu, Y S Zhang
Dairy goats are often fed a high-concentrate (HC) diet to meet their lactation demands; however, long-term concentrate feeding is unhealthy and leads to milk yield and lactose content decreases. Therefore, we tested whether a buffering agent is able to increase the output of glucose in the liver and influence lactose synthesis. Eight lactating goats were randomly assigned to two groups: one group received a HC diet (Concentrate : Forage = 6:4, HG) and the other group received the same diet with a buffering agent added (0...
January 5, 2018: Physiological Research
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