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https://www.readbyqxmd.com/read/29773074/enhanced-cellulase-production-in-trichoderma-reesei-rut-c30-via-constitution-of-minimal-transcriptional-activators
#1
Jiajia Zhang, Guoxiu Zhang, Wei Wang, Wei Wang, Dongzhi Wei
BACKGROUND: Cellulase can convert lignocellulosic feedstocks into fermentable sugars, which can be used for the industrial production of biofuels and chemicals. The high cost of cellulase production remains a challenge for lignocellulose breakdown. Trichoderma reesei RUT C30 serves as a well-known industrial workhorse for cellulase production. Therefore, the enhancement of cellulase production by T. reesei RUT C30 is of great importance. RESULTS: Two sets of novel minimal transcriptional activators (DBDace2 -VP16 and DBDcre1 -VP16) were designed and expressed in T...
May 17, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29772827/physicochemical-nutritional-and-organoleptic-characterization-of-a-skimmed-goat-milk-fermented-with-the-probiotic-strain-lactobacillus-plantarum-c4
#2
Miriam Moreno-Montoro, Miguel Navarro-Alarcón, Triana Bergillos-Meca, Rafael Giménez-Martínez, Silvia Sánchez-Hernández, Manuel Olalla-Herrera
The benefits of goat milk, fermented milks, and probiotics for the humans are well documented. In this study, a novel fermented goat milk was manufactured with the putative probiotic strain Lactobacillus plantarum C4 together with L. bulgaricus and Streptococcus thermophilus . Ultrafiltration was chosen as the skimmed milk concentration method because it produced the best viscosity and syneresis and a high casein content. The viability rate of all bacterial strains was >10⁷ cfu/mL, even after 5 weeks of storage or after in vitro gastrointestinal digestion, which is especially important for exertion of the probiotic strain functionalities...
May 17, 2018: Nutrients
https://www.readbyqxmd.com/read/29767155/responses-of-milk-production-of-dairy-cows-to-jugular-infusions-of-a-mixture-of-essential-amino-acids-with-or-without-exclusion-leucine-or-arginine
#3
Wen Tian, Tianyou Wu, Rui Zhao, Jinhao Xu, Yao He, Hongrong Wang
The purpose of this study was to determine effects of jugular infusion of either balanced or imbalanced amino acid mixture on milk production and composition in dairy cows. Eight mid-lactation Holstein cows were randomly assigned to 5-d continuous jugular infusions of saline (CTL), essential amino acid (EAA) mixture prepared on the profile of casein (CSN, 160 g/d), EAA mixture excluding leucine (Leu) (-Leu, 163 g/d) or EAA mixture excluding arginine (Arg) (-Arg, 158 g/d) in a duplicated 4 × 4 Latin square design with 4 infusion periods separated by a 7-d interval period...
September 2017: Animal nutrition
https://www.readbyqxmd.com/read/29766694/analysis-of-the-choice-of-food-products-and-the-energy-value-of-diets-of-female-middle-and-long-distance-runners-depending-on-the-self-assessment-of-their-nutritional-habits
#4
Dominika Głąbska, Marta Jusińska
Background: Properly balanced diet is especially important in the case of young athletes, as it influences not only their physical development, but also influences results obtained during trainings and competitions. Objective: The aim of the study was to assess the choice of food products and the energy value of diets of female middleand long-distance runners, depending on the self-assessment of their nutritional habits. Material and methods: The study was conducted in the group of 40 female middle- and long-distance runners, aged 15-25, who declared average diet (n=15, 37...
2018: Roczniki Państwowego Zakładu Higieny
https://www.readbyqxmd.com/read/29765446/inhibitory-effect-of-pdgf-bb-and-serum-stimulated-responses-in-vascular-smooth-muscle-cell-proliferation-by-hinokitiol-via-up-regulation-of-p21-and-p53
#5
Jiun-Yi Li, Chun-Ping Liu, Wei-Cheng Shiao, Thanasekaran Jayakumar, Yi-Shin Li, Nen-Chung Chang, Shih-Yi Huang, Cheng-Ying Hsieh
Introduction: Vascular smooth muscle cell (VSMC) proliferation plays a major role in the progression of vascular diseases. In the present study, we established the efficacy and the mechanisms of action of hinokitiol, a tropolone derivative found in Chamaecyparis taiwanensis , Cupressaceae, in relation to platelet-derived growth factor-BB (PDGF-BB) and serum-dependent VSMC proliferation. Material and methods: Primary cultured rat VSMCs were pre-treated with hinokitiol and then stimulated by PDGF-BB (10 ng/ml) or serum (10% fetal bovine serum)...
April 2018: Archives of Medical Science: AMS
https://www.readbyqxmd.com/read/29764491/does-a-low-fodmaps-diet-reduce-symptoms-of-functional-abdominal-pain-disorders-a-systematic-review-in-adult-and-paediatric-population-on-behalf-of-italian-society-of-pediatrics
#6
REVIEW
Rossella Turco, Silvia Salvatore, Erasmo Miele, Claudio Romano, Gian Luigi Marseglia, Annamaria Staiano
BACKGROUND: Despite the rising of the Functional Gastrointestinal Disorders (FGIDs)' incidence in the last years, the etio-pathogenesis of FGIDs remains unclear. The diet seems to play an important role in these disorders. Indeed, at least two thirds of adult patients with Irritable Bowel Syndrome (IBS) and of children with FGIDs perceive their GI symptoms to be food-related. In particular, in the last years, more interest has been focused in the low Fermentable Oligosaccharides, Disaccharides, Monosaccharides, and Polyol (FODMAPs) diet...
May 15, 2018: Italian Journal of Pediatrics
https://www.readbyqxmd.com/read/29762373/use-of-starch-and-modified-starches-in-infant-feeding-a-historical-perspective
#7
Frank R Greer
There is a long history of the use of starch in infant feeding. Proprietary infant foods (1867-1920) contained added starch from either cereal grains or malted carbohydrates. When evaporated milk became available in the 1920s, the use of proprietary foods fell out of favor. Evaporated milk formulas were a mixture of milk, water, and modified starch or milk sugar (lactose). By the late 1920s, however, corn syrup became the most common modified starch added to evaporated milk formulas as it was widely available, inexpensive, and readily accepted...
June 2018: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/29762009/wavelength-modulated-back-scatter-interferometry-for-universal-on-column-refractive-index-detection-in-picoliter-volumes
#8
Robert C Dunn
Wavelength modulated back scatter interferometry (M-BSI) is shown to improve the detection metrics for refractive index (RI) sensing in micro-separations. In M-BSI, the output of a tunable diode laser is focused into the detection zone of a separation channel as the excitation wavelength is rapidly modulated. This spatially modulates the observed interference pattern, which is measured in the back-scattered direction. Using a split photodiode detector aligned on one fringe of the of interference pattern, phase-sensitive detection is used to monitor RI changes as analytes are separated...
May 15, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29757067/a-comparative-study-of-the-influence-of-alpha-lactose-monohydrate-particle-morphology-on-granule-and-tablet-properties-after-roll-compaction-dry-granulation
#9
Simon Grote, Peter Kleinebudde
The influence of particle morphology and size of alpha-lactose monohydrate on dry granules and tablets was studied. Four different morphologies were investigated: Two grades of primary crystals, which differed in their particle size and structure (compact crystals vs. agglomerates). The materials were roll compacted at different specific compaction forces and changes in the particle size distribution and the specific surface area were measured. Afterwards two fractions of granules were pressed to tablets and the tensile strength was compared to that from tablets compressed from the raw materials...
May 14, 2018: Pharmaceutical Development and Technology
https://www.readbyqxmd.com/read/29753479/production-of-calcium-and-magnesium-enriched-caseins-and-caseinates-by-an-ecofriendly-technology
#10
Félix-André Masson, Sergey Mikhaylin, Laurent Bazinet
Finding new green ways of producing proteins has never been of such critical public interest, both to meet consumers' needs and to preserve the environment. Milk proteins are among the most attractive protein types due to their high nutritional value and attractive functional properties. In this work, the separation of caseins by conventional chemical acidification was compared with electrodialysis with bipolar membrane coupled to an ultrafiltration module (EDBM-UF), a green process that allows the precipitation of caseins by H+ generated in situ by the bipolar membrane and, simultaneously, the production of a separated NaOH stream from OH- electrogenerated by the bipolar membrane...
May 9, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29753468/effects-of-phytonutrients-alone-or-in-combination-with-monensin-on-productivity-in-lactating-dairy-cows
#11
J Oh, M Harper, C H Lang, E H Wall, A N Hristov
This experiment was conducted to investigate the effects of phytonutrients, compared with monensin as a positive control, on productivity, milk fatty acids, fat mobilization, and blood cells in lactating dairy cows. Thirty-six Holstein cows were used in a 9-wk randomized complete block design study. Following a 2-wk covariate period, cows were blocked by days in milk, parity, and milk yield and randomly assigned to 1 of 3 treatments (12 cows/treatment): 450 mg/cow per day of monensin (MO), 250 mg/cow per day of capsicum plus 450 mg/cow per day of MO (MOCAP), and 1,000 mg/cow per day of a mixture of cinnamaldehyde, eugenol, and capsicum (CEC)...
May 9, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29753467/effect-of-exogenous-amylase-on-lactation-performance-of-dairy-cows-fed-a-high-starch-diet
#12
Arturo S R Andreazzi, Marcos N Pereira, Ronaldo B Reis, Renata A N Pereira, Nilson N Morais Júnior, Tiago S Acedo, Rafael G Hermes, Cristina S Cortinhas
Exogenous amylase supplementation can increase starch and fiber digestibility in lactating dairy cows. We evaluated the effect of exogenous amylase supplementation on diets with high starch concentration (32% of dry matter). Twenty-eight Holstein cows (171 ± 80 d in milk, 4 primiparous) received a standard diet for 14 d and then a treatment for 63 d, in a covariate-adjusted randomized block design with repeated measures over time. Treatments were amylase [0.5 g of Ronozyme RumiStar (DSM Nutritional Products, Basel, Switzerland) per kg of total mixed ration dry matter] or control...
May 9, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29753466/milk-yield-quality-and-coagulation-properties-of-6-breeds-of-goats-environmental-and-individual-variability
#13
Giuseppe M Vacca, Giorgia Stocco, Maria L Dettori, Emanuela Pira, Giovanni Bittante, Michele Pazzola
Goat milk and cheese production is continuously increasing and milk composition and coagulation properties (MCP) are useful tools to predict cheesemaking aptitude. The present study was planned to investigate the extension of lactodynamographic analysis up to 60 min in goat milk, to measure the farm and individual factors, and to investigate differences among 6 goat breeds. Daily milk yield (dMY) was recorded and milk samples collected from 1,272 goats reared in 35 farms. Goats were of 6 different breeds: Saanen and Camosciata delle Alpi for the Alpine type, and Murciano-Granadina, Maltese, Sarda, and Sarda Primitiva for the Mediterranean type...
May 9, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29752268/preferred-hexoses-influence-long-term-memory-and-induction-of-lactose-catabolism-by-streptococcus-mutans
#14
Lin Zeng, Lulu Chen, Robert A Burne
Bacteria prioritize sugar metabolism via carbohydrate catabolite repression, which regulates global gene expression to optimize the catabolism of preferred substrates. Here, we report an unusual long-term memory effect in certain Streptococcus mutans strains that alters adaptation to growth on lactose after prior exposure to glucose or fructose. In strain GS-5, cells that were first cultured on fructose then transferred to lactose displayed an exceptionally long lag (>11 h) and slower growth, compared to cells first cultured on glucose or cellobiose, which displayed a reduction in lag phase by as much as 10 h...
May 11, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29752082/pvc-membrane-coated-wire-and-carbon-paste-ion-selective-electrodes-for-potentiometric-determination-of-galantamine-hydrobromide-in-physiological-fluids
#15
Fatehy M Abdel-Haleem, Mohamed Saad, Ahmed Barhoum, Mikhael Bechelany, Mahmoud S Rizk
We report on highly-sensitive ion-selective electrodes (ISEs) for potentiometric determining of galantamine hydrobromide (GB) in physiological fluids. Galantamine hydrobromide (GB) was selected for this study due to its previous medical importance for treating Alzheimer's disease. Three different types of ISEs were investigated: PVC membrane electrode (PVCE), carbon-paste electrode (CPE), and coated-wire electrode (CWE). In the construction of these electrodes, galantaminium-reineckate (GR) ion-pair was used as a sensing species for GB in solutions...
August 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/29747128/supramolecular-nanofiber-of-pyrene-lactose-conjugates-and-its-two-photon-fluorescence-imaging
#16
Qian Sun, Hong-Yu Zhu, Jun-Fang Wang, Xiao Chen, Ke-Rang Wang, Xiao-Liu Li
A lactose modified pyrene derivative (Py-Lac) was synthesized, with which novel twisted supramolecular nanofibers in diameter about 20 nm were constructed by self-assembly. The nanofibers showed solid-state fluorescence between 400 nm and 650 nm with the maximum emission at 495 nm. Furthermore, its recognition reaction with PNA lectin was investigated by fluorescence spectra and turbidity assays. It is interesting found that the supramolecular assembly as multivalent glycocluster exhibited unique and selectively binding interactions with PNA lectin with the binding constant of 5...
April 23, 2018: Bioorganic Chemistry
https://www.readbyqxmd.com/read/29745848/automated-transition-analysis-of-activated-gene-regulation-during-diauxic-nutrient-shift-in-escherichia-coli-and-adipocyte-differentiation-in-mouse-cells
#17
Yoichi Takenaka, Kazuma Mikami, Shigeto Seno, Hideo Matsuda
BACKGROUND: Comprehensively understanding the dynamics of biological systems is among the biggest current challenges in biology and medicine. To acquire this understanding, researchers have measured the time-series expression profiles of cell lines of various organisms. Biological technologies have also drastically improved, providing a huge amount of information with support from bioinformatics and systems biology. However, the transitions between the activation and inactivation of gene regulations, at the temporal resolution of single time points, are difficult to extract from time-course gene expression profiles...
May 8, 2018: BMC Bioinformatics
https://www.readbyqxmd.com/read/29743471/optimization-of-premix-powders-for-tableting-use
#18
Hiroaki Todo, Kazuki Sato, Kozo Takayama, Kenji Sugibayashi
Direct compression is a popular choice as it provides the simplest way to prepare the tablet. It can be easily adopted when the active pharmaceutical ingredient (API) is unstable in water or to thermal drying. An optimal formulation of preliminary mixed powders (premix powders) is beneficial if prepared in advance for tableting use. The aim of this study was to find the optimal formulation of the premix powders composed of lactose (LAC), cornstarch (CS), and microcrystalline cellulose (MCC) by using statistical techniques...
May 8, 2018: Chemical & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/29740476/milk-composition-for-admixed-dairy-cattle-in-tanzania
#19
Evans K Cheruiyot, Rawlynce C Bett, Joshua O Amimo, Fidalis D N Mujibi
It is well established that milk composition is affected by the breed and genotype of a cow. The present study investigated the relationship between the proportion of exotic genes and milk composition in Tanzanian crossbred dairy cows. Milk samples were collected from 209 animals kept under smallholder production systems in Rungwe and Lushoto districts of Tanzania. The milk samples were analyzed for the content of components including fat, protein, casein, lactose, solids-not-fat (SNF), and the total solids (TS) through infrared spectroscopy using Milko-Scan FT1 analyzer (Foss Electric, Denmark)...
2018: Frontiers in Genetics
https://www.readbyqxmd.com/read/29740087/the-lactose-operon-from-lactobacillus-casei-is-involved-in-the-transport-and-metabolism-of-the-human-milk-oligosaccharide-core-2-n-acetyllactosamine
#20
Gonzalo N Bidart, Jesús Rodríguez-Díaz, Gaspar Pérez-Martínez, María J Yebra
The lactose operon (lacTEGF) from Lactobacillus casei strain BL23 has been previously studied. The lacT gene codes for a transcriptional antiterminator, lacE and lacF for the lactose-specific phosphoenolpyruvate: phosphotransferase system (PTSLac ) EIICB and EIIA domains, respectively, and lacG for the phospho-β-galactosidase. In this work, we have shown that L. casei is able to metabolize N-acetyllactosamine (LacNAc), a disaccharide present at human milk and intestinal mucosa. The mutant strains BL153 (lacE) and BL155 (lacF) were defective in LacNAc utilization, indicating that the EIICB and EIIA of the PTSLac are involved in the uptake of LacNAc in addition to lactose...
May 8, 2018: Scientific Reports
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