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https://www.readbyqxmd.com/read/29040969/comparison-of-igh-profile-signals-using-t-4-14-and-igh-break-apart-probes-by-fish-in-multiple-myeloma
#1
Thomas Smol, Agnès Daudignon
We compared immunoglobulin heavy chain gene (IGH) signal patterns in multiple myeloma (MM) using the FGFR3-IGH and the IGH break-apart probes to facilitate their understanding and analysis. Forty-nine patients with MM were studied. FISH was performed on samples sorted with an FGFR3-IGH dual-color, dual-fusion translocation probe and an IGH dual-color break-apart rearrangement probe. The IGH deletions were found in 7 MM analyzed with the FGFR3-IGH probe and all confirmed by the IGH break-apart probe. The additional IGH signals were associated with different patterns using the IGH break-apart probe: a normal pattern in 9 cases, trisomy 14 in 3 cases, and splits of IGH in 7 cases...
October 18, 2017: Cytogenetic and Genome Research
https://www.readbyqxmd.com/read/29040807/photochemical-control-over-oscillations-in-chemical-reaction-networks
#2
Aleksandr A Pogodaev, Albert S Y Wong, Wilhelm T S Huck
Systems chemistry aims to emulate the functional behavior observed in living systems by constructing chemical reaction networks (CRNs) with well-defined dynamic properties. Future expansion of the complexity of these systems would require external control to tune behavior and temporal organization of such CRNs. In this work, we design and implement a photolabile probe, which upon irradiation strengthens the negative feedback loop of a CRN that produces oscillations of trypsin under out-of-equilibrium conditions...
October 17, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29040803/synthesis-and-characterization-of-fatty-acid-grafted-chitosan-polymer-and-their-nanomicelles-for-non-viral-gene-delivery-applications
#3
Divya Sharma, Jagdish Singh
The aim of this study was to synthesize and characterize fatty acid-grafted-chitosan (fatty acid-g-CS) polymer and their nanomicelles for using as carriers for gene delivery. CS was hydrophobically modified using saturated fatty acids of increasing fatty acyl chain length. Carbodiimide along with N-hydroxysuccinimide was used for coupling carboxyl group of fatty acids with amine groups of CS. Proton nuclear magnetic resonance and Fourier transform-infrared spectroscopy were used to quantify fatty acyl substitution onto CS backbone...
October 17, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/29040720/genome-wide-prediction-of-minor-groove-electrostatic-potential-enables-biophysical-modeling-of-protein-dna-binding
#4
Tsu-Pei Chiu, Satyanarayan Rao, Richard S Mann, Barry Honig, Remo Rohs
Protein-DNA binding is a fundamental component of gene regulatory processes, but it is still not completely understood how proteins recognize their target sites in the genome. Besides hydrogen bonding in the major groove (base readout), proteins recognize minor-groove geometry using positively charged amino acids (shape readout). The underlying mechanism of DNA shape readout involves the correlation between minor-groove width and electrostatic potential (EP). To probe this biophysical effect directly, rather than using minor-groove width as an indirect measure for shape readout, we developed a methodology, DNAphi, for predicting EP in the minor groove and confirmed the direct role of EP in protein-DNA binding using massive sequencing data...
October 11, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29040675/fluctuation-localization-imaging-based-fluorescence-in-situ-hybridization-flifish-for-accurate-detection-and-counting-of-rna-copies-in-single-cells
#5
Yi Cui, Dehong Hu, Lye Meng Markillie, William B Chrisler, Matthew J Gaffrey, Charles Ansong, Lori Sussel, Galya Orr
Quantitative gene expression analysis in intact single cells can be achieved using single molecule-based fluorescence in situ hybridization (smFISH). This approach relies on fluorescence intensity to distinguish between true signals, emitted from an RNA copy hybridized with multiple oligonucleotide probes, and background noise. Thus, the precision in smFISH is often compromised by partial or nonspecific probe binding and tissue autofluorescence, especially when only a small number of probes can be fitted to the target transcript...
October 4, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29040662/molecular-subtyping-of-tumors-from-patients-with-familial-glioma
#6
Vanessa Y Ruiz, Corinne E Praska, Georgina Armstrong, Thomas M Kollmeyer, Seiji Yamada, Paul A Decker, Matthew L Kosel, Jeanette E Eckel-Passow, The Gliogene Consortium, Daniel H Lachance, Matthew N Bainbridge, Beatrice S Melin, Melissa L Bondy, Robert B Jenkins
BACKGROUND: Single-gene mutation syndromes account for some familial glioma (FG); however, they make up only a small fraction of glioma families. Gliomas can be classified into 3 major molecular subtypes based on IDH mutation and 1p/19q co-deletion. We hypothesized that the prevalence of molecular subtypes might differ in familial versus sporadic gliomas, and that tumors in the same family should have the same molecular subtype. METHODS: Participants in the FG study (Gliogene) provided samples for germline DNA analysis...
October 10, 2017: Neuro-oncology
https://www.readbyqxmd.com/read/29040344/dilemmas-for-guardians-of-advanced-dementia-patients-regarding-tube-feeding
#7
Efrat Gil, Maayan Agmon, Ayal Hirsch, Miriam Ziv, Anna Zisberg
Background: advanced dementia is an incurable illness, its last stage marked by inability to eat. Tube feeding was deemed a helpful solution at this stage, but in recent years its inefficiency has been proved, and it is no longer practiced in many countries around the world. In Israel, however, the procedure is still accepted. In the gastroenterology department at the Bnai Zion Medical Center, a serious interaction is ongoing with patients' legal guardians, where detailed information is given about the inefficiency of the tube procedure...
October 13, 2017: Age and Ageing
https://www.readbyqxmd.com/read/29040279/social-exclusion-leads-to-attentional-bias-to-emotional-social-information-evidence-from-eye-movement
#8
Zhuohao Chen, Jinchen Du, Min Xiang, Yan Zhang, Shuyue Zhang
Social exclusion has many effects on individuals, including the increased need to belong and elevated sensitivity to social information. Using a self-reporting method, and an eye-tracking technique, this study explored people's need to belong and attentional bias towards the socio-emotional information (pictures of positive and negative facial expressions compared to those of emotionally-neutral expressions) after experiencing a brief episode of social exclusion. We found that: (1) socially-excluded individuals reported higher negative emotions, lower positive emotions, and stronger need to belong than those who were not socially excluded; (2) compared to a control condition, social exclusion caused a longer response time to probe dots after viewing positive or negative face images; (3) social exclusion resulted in a higher frequency ratio of first attentional fixation on both positive and negative emotional facial pictures (but not on the neutral pictures) than the control condition; (4) in the social exclusion condition, participants showed shorter first fixation latency and longer first fixation duration to positive pictures than neutral ones but this effect was not observed for negative pictures; (5) participants who experienced social exclusion also showed longer gazing duration on the positive pictures than those who did not; although group differences also existed for the negative pictures, the gaze duration bias from both groups showed no difference from chance...
2017: PloS One
https://www.readbyqxmd.com/read/29040148/long-term-effects-of-bone-morphogenetic-protein-2-loaded-calcium-phosphate-on-maxillary-sinus-lift-surgery-for-delayed-and-simultaneous-dental-implantation
#9
Yuefeng Ding, Xuejuan Wang
Maxillary sinus lift surgery (MSLS) is considered to be a useful treatment method for patients with atrophic alveolar bone. Choosing a suitable surgical procedure to simultaneously decrease graft absorption and obtain long-term survival of dental implants is still a challenge. In this study, 20 patients received MSLS with graft of bone morphogenetic protein 2 (BMP2)-loaded calcium phosphate. Dental implants were placed simultaneously in 10 patients receiving MSLS (1-stage group), and in 10 patients receiving dental implants with a 3 to 6 months delay (2-stage group)...
October 16, 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/29040026/a-comparison-of-the-success-rates-of-endoscopic-assisted-probing-in-the-treatment-of-membranous-congenital-nasolacrimal-duct-obstruction-between-younger-and-older-children-and-its-correlation-with-the-thickness-of-the-membrane-at-the-valve-of-hasner
#10
Nishi Gupta, Chawla Neeraj, Bansal Smriti, Das Sima
Congenital nasolacrimal duct obstruction (CNLDO) remains the most common cause of epiphora in infants. This retrospective study compares the success rate of nasal endoscopic-assisted probing between younger (3 years and below) and older (above 3 years) children with membranous CNLDO and its correlation with the thickness of the membrane at the valve of Hasner. Case records of a total of 38 eyes in 34 children with membranous CNLDO who underwent endoscopic nasolacrimal duct probing and irrigation under general anesthesia were analyzed...
October 17, 2017: Orbit
https://www.readbyqxmd.com/read/29040007/probing-birth-order-effects-on-narrow-traits-using-specification-curve-analysis
#11
Julia M Rohrer, Boris Egloff, Stefan C Schmukle
The idea that birth-order position has a lasting impact on personality has been discussed for the past 100 years. Recent large-scale studies have indicated that birth-order effects on the Big Five personality traits are negligible. In the current study, we examined a variety of more narrow personality traits in a large representative sample ( n = 6,500-10,500 in between-family analyses; n = 900-1,200 in within-family analyses). We used specification-curve analysis to assess evidence for birth-order effects across a range of models implementing defensible yet arbitrary analytical decisions (e...
October 1, 2017: Psychological Science
https://www.readbyqxmd.com/read/29039964/probing-linewidths-and-biexciton-quantum-yields-of-single-cesium-lead-halide-nanocrystals-in-solution
#12
Hendrik Utzat, Katherine E Shulenberger, Odin B Achorn, Michel Nasilowski, Timothy S Sinclair, Moungi G Bawendi
Cesium lead halide (CsPbX3, X = Cl, Br, I) perovskite nanocrystals (PNCs) have recently become a promising material for optoelectronic applications due to their high emission quantum yields and facile band gap tunability via both halide composition and size. The spectroscopy of single PNCs enhances our understanding of the effect of confinement on excitations in PNCs in the absence of obfuscating ensemble averaging and can also inform synthetic efforts. However, single PNC studies have been hampered by poor PNC photostability under confocal excitation, precluding interrogation of all but the most stable PNCs, leading to a lack of understanding of PNCs in the regime of high confinement...
October 17, 2017: Nano Letters
https://www.readbyqxmd.com/read/29039949/highly-efficient-red-emitting-carbon-dots-with-gram-scale-yield-for-bioimaging
#13
Hui Ding, Ji-Shi Wei, Ning Zhong, Qingyu Gao, Huan-Ming Xiong
Carbon dots (CDs) are a new class of photoluminescent (PL), biocompatible, environment friendly and low-cost carbon nanomaterials. Synthesis of highly efficient red-emitting carbon dots (R-CDs) on a gram scale is a great challenge at present, which heavily restricts the wide applications of CDs in the bioimaging field. Herein, R-CDs with a high quantum yield (QY) of 53% are produced on a gram scale by heating a formamide solution of citric acid and ethylenediamine. The as-prepared R-CDs have an average size of 4...
October 17, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29039937/concentration-independent-stereodynamic-g-probe-for-chiroptical-enantiomeric-excess-determination
#14
Paolo Zardi, Klaus Wurst, Giulia Licini, Cristiano Zonta
Enantiomeric excess determination is crucial in many aspects of science ranging from synthesis to materials. Within this subject, the use of molecular sensors coupled with chiroptical techniques is a straightforward approach to the stereochemical analysis of chiral molecules, especially in terms of process immediacy and labor. Ste-reodynamic probes typically consist of racemic mixtures of rapidly interconverting enantiomeric conformers able to recognize a chiral analyte and greatly amplify its chiroptical readout...
October 17, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29039920/shedding-light-on-the-photo-isomerization-pathway-of-donor-acceptor-stenhouse-adducts
#15
Mariangela Di Donato, Michael M Lerch, Andrea Lapini, Adèle D Laurent, Alessandro Iagatti, Laura Bussotti, Svante P Ihrig, Miroslav Medved, Denis Jacquemin, Wiktor Szymanski, Wybren Jan Buma, Paolo Foggi, Ben L Feringa
Donor-acceptor Stenhouse adducts (DASAs) are negative photochromes that hold great promise for a variety of applications. Key to optimizing their switching properties is a detailed understanding of the photoswitching mechanism, which, as yet, is absent. Here we characterize the actinic step of DASA-photoswitching and its key intermediate, which was studied using a combination of ultrafast visible and IR pump-probe spectroscopies and TD-DFT calculations. Comparison of the time-resolved IR spectra with DFT computations allowed to unambiguously identify the structure of the intermediate, confirming that light absorption induces a sequential reaction path in which a Z-E photoisomerization of C2-C3 is followed by a rotation around C3-C4 and subsequent thermal cyclization step...
October 17, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29036827/validation-by-magnetic-resonance-imaging-of-the-diagnostic-potential-of-a-heptapeptide-functionalized-imaging-probe-targeted-to-amyloid-%C3%AE-and-able-to-cross-the-blood-brain-barrier
#16
Séverine André, Emilie Ansciaux, Elamine Saidi, Lionel Larbanoix, Dimitri Stanicki, Denis Nonclercq, Luce Vander Elst, Sophie Laurent, Robert N Muller, Carmen Burtea
The diagnosis of Alzheimer's disease (AD) is a critical step in the management of patients. We have developed a non-invasive diagnosis tool based on magnetic resonance molecular imaging (MRMI) of amyloid-β peptide using ultra-small particles of iron oxide (USPIO) functionalized with a disulfide constrained cyclic heptapeptide (PHO) identified by phage display (USPIO-PHO). After previously demonstrating the optimal pharmacologic properties of USPIO-PHO and its capacity to cross the blood-brain barrier (BBB), the ability of USPIO-PHO to target amyloid plaques (AP) by MRMI has been validated in the present work on AD transgenic mice...
October 12, 2017: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/29036704/measurements-of-airborne-concentrations-of-radon-and-thoron-decay-products
#17
S Chalupnik, K Skubacz, P Urban, M Wysocka
Liquid scintillation counting (LSC) is a measuring technique, broadly applied in environmental monitoring of radionuclides. One of the possible applications of LSC is the measurement of radon and thoron decay products. But this method is suitable only for grab sampling. For long-term measurements a different technique can be applied-monitors of potential alpha energy concentration (PAEC) with thermoluminescent detectors (TLD). In these devices, called Alfa-2000 sampling probe, TL detectors (CaSO4:Dy) are applied for alpha particles counting...
September 19, 2017: Radiation Protection Dosimetry
https://www.readbyqxmd.com/read/29036687/measuring-deaminated-nucleotide-surveillance-enzyme-itpa-activity-with-an-atp-releasing-nucleotide-chimera
#18
Debin Ji, Elena I Stepchenkova, Jian Cui, Miriam R Menezes, Youri I Pavlov, Eric T Kool
Nucleotide quality surveillance enzymes play important roles in human health, by detecting damaged molecules in the nucleotide pool and deactivating them before they are incorporated into chromosomal DNA or adversely affect metabolism. In particular, deamination of adenine moiety in (deoxy)nucleoside triphosphates, resulting in formation of (d)ITP, can be deleterious, leading to DNA damage, mutagenesis and other harmful cellular effects. The 21.5 kDa human enzyme that mitigates this damage by conversion of (d)ITP to monophosphate, ITPA, has been proposed as a possible therapeutic and diagnostic target for multiple diseases...
September 28, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29036619/the-effects-of-dna-supercoiling-on-g-quadruplex-formation
#19
Doreen A T Sekibo, Keith R Fox
Guanine-rich DNAs can fold into four-stranded structures that contain stacks of G-quartets. Bioinformatics studies have revealed that G-rich sequences with the potential to adopt these structures are unevenly distributed throughout genomes, and are especially found in gene promoter regions. With the exception of the single-stranded telomeric DNA, all genomic G-rich sequences will always be present along with their C-rich complements, and quadruplex formation will be in competition with the corresponding Watson-Crick duplex...
September 28, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29036582/characterization-of-clyc-scintillator-coupled-with-photomultipliers-and-a-large-sipm-array
#20
N Dinar, D Celeste, P Puzo, M Silari
CERN Radiation Protection group has recently developed a novel radiation survey meter called B-RAD able to operate in the presence of a strong magnetic field. The B-RAD will be equipped with a series of probes for gamma dose rate, gamma spectrometry and surface contamination measurements. The feasibility of developing a probe for neutron dose rate and possibly spectral measurements is being investigated. The determination of the breakdown voltage of the SiPM as well as its uniformity between the pixels was performed...
September 29, 2017: Radiation Protection Dosimetry
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