keyword
MENU ▼
Read by QxMD icon Read
search

Probe

keyword
https://www.readbyqxmd.com/read/29454281/probing-the-%C3%AE-pocket-of-the-active-site-of-human-liver-glycogen-phosphorylase-with-3-c-%C3%AE-d-glucopyranosyl-5-4-substituted-phenyl-1-2-4-triazole-inhibitors
#1
Efthimios Kyriakis, Theodora G A Solovou, Sándor Kun, Katalin Czifrák, Béla Szőcs, László Juhász, Éva Bokor, George A Stravodimos, Anastassia L Kantsadi, Demetra S M Chatzileontiadou, Vassiliki T Skamnaki, László Somsák, Demetres D Leonidas
Human liver glycogen phosphorylase (hlGP), a key enzyme in glycogen metabolism, is a valid pharmaceutical target for the development of new anti-hyperglycaemic agents for type 2 diabetes. Inhibitor discovery studies have focused on the active site and in particular on glucopyranose based compounds with a β-1 substituent long enough to exploit interactions with a cavity adjacent to the active site, termed the β-pocket. Recently, C-β-d-glucopyranosyl imidazoles and 1, 2, 4-triazoles proved to be the best known glucose derived inhibitors of hlGP...
February 12, 2018: Bioorganic Chemistry
https://www.readbyqxmd.com/read/29454250/disulphide-linkage-to-get-cleaved-or-not-bulk-and-nano-copper-based-sers-of-cystine
#2
Arathi P J, Bhaskar Seemesh, Rajendra Kumar Reddy G, Suresh Kumar P, Ramanathan V
Different nano-structures of noble metals have been the conventional substrates for carrying out Surface Enhanced Raman Spectroscopy (SERS). In this paper we examine electrodeposited copper (Cu) nano-structures on pencil graphite as novel substrate to carry out SERS measurements by considering l-cystine (Cys-Cys) (dimer of the amino acid cysteine) as the probe. The formation of monolayer of the probe molecule on the substrates was confirmed using cyclic voltammetric measurements. Mode of adsorption of Cys-Cys was observed to be different on bulk Cu (taken in the wire form) and nano-structured Cu on pencil graphite...
February 5, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29454238/transformations-of-dissolved-organic-matter-induced-by-uv-photolysis-hydroxyl-radicals-chlorine-radicals-and-sulfate-radicals-in-aqueous-phase-uv-based-advanced-oxidation-processes
#3
Lathika Varanasi, Erica Coscarelli, Maryam Khaksari, Lynn R Mazzoleni, Daisuke Minakata
Considering the increasing identification of trace organic contaminants in natural aquatic environments, the removal of trace organic contaminants from water or wastewater discharge is an urgent task. Ultraviolet (UV) and UV-based advanced oxidation processes (AOPs), such as UV/hydrogen peroxide (UV/H 2 O 2 ), UV/free chlorine and UV/persulfate, are attractive and promising approaches for the removal of these contaminants due to the high reactivity of active radical species produced in these UV-AOPs with a wide variety of organic contaminants...
February 8, 2018: Water Research
https://www.readbyqxmd.com/read/29454192/phytophthora-sojae-effectors-orchestrate-warfare-with-host-immunity
#4
REVIEW
Yan Wang, Yuanchao Wang
Phytophthora sojae is one of the most damaging plant pathogens of soybean. To aid establishment of a compatible interaction with its host, P. sojae deploys many secreted effectors. These effectors act either in the apoplastic space to cope with hostile conditions or inside of host cells to reprogram host physiology favoring pathogen growth. Effectors have been used as molecular probes, which revealed in Phytophthora that effectors execute their virulence function via manipulating host targets. In addition, recent studies have discovered 'pseudo-effectors' in Phytophthora that act as decoys to shield virulence effectors from host defense, a new paradigm in plant-pathogen interactions...
February 14, 2018: Current Opinion in Microbiology
https://www.readbyqxmd.com/read/29454148/-68-ga-galmydar-biodistribution-and-radiation-dosimetry-studies-in-rodents
#5
Jothilingam Sivapackiam, Richard Laforest, Vijay Sharma
INTRODUCTION: 68 Ga-Galmydar is an avid transport substrate of ABCB1 (P-Glycoprotein; 170kDa plasma membrane protein), breast cancer resistance protein (BCRP; ABCG2; 72kDa), penetrates human epidermal carcinoma (KB3-1), breast cancer (MCF7), embryonic kidney (HEK 293) tumor cells and rat cardiomyoblasts, and localizes within the mitochondria of tumor and myocardium cells. 68 Ga-Galmydar excretes from blood pool quickly, and shows stable retention within rat myocardium in vivo for extended periods, therefore, the agent shows potential to enable myocardial perfusion imaging...
December 1, 2017: Nuclear Medicine and Biology
https://www.readbyqxmd.com/read/29454117/the-differentially-expressed-genes-of-human-sporadic-cerebral-cavernous-malformations
#6
Xiangjiang Lin, Guolu Meng, Xing Liu, Tengfei Yu, Chuanfeng Bai, Xiaobin Fei, Shengze Deng, Jizong Zhao, Shulin Ren, Junting Zhang, Zhen Wu, Shuo Wang, Jianguo Zhang, Liwei Zhang
OBJECTIVES: To understand the development of Sporadic cerebral cavernous malformations (SCCM) comprehensively, we analyzed gene expression profiles in SCCMs by the gene microarray. METHODS: The total number of the specimens collected in our study was 14, 7 of which were SCCMs, and the others were controls that were obtained from normal brain vessels. The total RNA was extracted and hybridized with Oligonucleotide array containing 21522 genes. The analysis of Gene Ontology (GO) items and molecular pathways was performed based on the GO and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases...
February 14, 2018: World Neurosurgery
https://www.readbyqxmd.com/read/29454072/killer-cell-immunoglobulin-like-receptor-genotypes-in-the-turkish-population
#7
Mehmet Onur Elbaşi, Aysın Tulunay-Virlan, Emel Ekşioğlu-Demiralp
One hundred eighty-seven healthy and unrelated volunteers from various regions of Turkey were selected for the study. Killer-cell immunoglobulin-like receptors (KIR) genotyping was performed by polymerase chain reaction using commercial sequence-specific oligonucleotide probe (SSOP) kits. Gene frequencies of the Turkish population were determined by direct counting of the positive and negative loci. The genotype data is publicly available in the Allele Frequencies Net Database under the population name "Turkey KIR pop 3" number "3399"...
February 14, 2018: Human Immunology
https://www.readbyqxmd.com/read/29453903/tracking-the-dynamic-folding-and-unfolding-of-rna-g-quadruplexes-in-live-cells
#8
Xiu-Cai Chen, Shuo-Bin Chen, Jing Dai, Jia-Hao Yuan, Tian-Miao Ou, Zhi-Shu Huang, Jia-Heng Tan
Due to the absence of methods for tracking RNA G-quadruplex dynamics, especially the folding and unfolding of this attractive structure in live cells, understanding of the biological roles of RNA G-quadruplexes is so far limited. Here we reported a new red-emitting fluorescent probe, QUMA-1, for a selective, continuous and real-time visualization of RNA G-quadruplexes in live cells. The applications of QUMA-1 in several previously intractable applications, including live-cell imaging of the dynamic folding, unfolding and movement of RNA G-quadruplexes and visualizing the unwinding of RNA G-quadruplexes by RNA helicase have been demonstrated...
February 17, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/29453775/effect-of-pparg-on-ages-induced-akt-mtor-signaling-associated-human-chondrocytes-autophagy
#9
Zhao-Jun Wang, Hai-Bin Zhang, Cheng Chen, Hao Huang, Jian-Xia Liang
Accumulation of advanced glycation end products (AGEs) in articular cartilage is thought to represent a major risk factor for osteoarthritis development. In this study we aimed to probe the role of AGEs in human chondrocytes and to determine the impact of the peroxisome proliferator-activated receptor-γ (PPARG) on AGEs-induced cell autophagy. Cell viability was measured after human chondrocytes were treated with different concentrations of AGEs with or without the PPARG inhibitor, T0070907, or agonist, pioglitazone...
February 17, 2018: Cell Biology International
https://www.readbyqxmd.com/read/29453705/using-peer-mediated-lego%C3%A2-play-intervention-to-improve-social-interactions-for-chinese-children-with-autism-in-an-inclusive-setting
#10
Xiaoyi Hu, Qunshan Zheng, Gabrielle T Lee
The purpose of this study was to examine the effects of a peer-mediated LEGO® play intervention on improving social skills for children with ASD in an inclusive preschool in China. Three boys with ASD and 13 typically developing children participated in this study. A multiple-probe across participants design was used. The intervention consisted of LEGO® construction activities incorporated with peer-mediated strategies for one child with ASD and two typically developing peers. The intervention sessions were conducted two sessions per week with a total of 28-31 sessions for each participant...
February 16, 2018: Journal of Autism and Developmental Disorders
https://www.readbyqxmd.com/read/29453662/the-overexpression-of-rxam1-a-cassava-gene-coding-for-an-rlk-confers-disease-resistance-to-xanthomonas-axonopodis-pv-manihotis
#11
Paula A Díaz Tatis, Mariana Herrera Corzo, Juan C Ochoa Cabezas, Adriana Medina Cipagauta, Mónica A Prías, Valerie Verdier, Paul Chavarriaga Aguirre, Camilo E López Carrascal
The overexpression of RXam1 leads to a reduction in bacterial growth of XamCIO136, suggesting that RXam1 might be implicated in strain-specific resistance. Cassava bacterial blight (CBB) caused by Xanthomonas axonopodis pv. manihotis (Xam) is a prevalent disease in all regions, where cassava is cultivated. CBB is a foliar and vascular disease usually controlled through host resistance. Previous studies have found QTLs explaining resistance to several Xam strains. Interestingly, one QTL called XM5 that explained 13% of resistance to XamCIO136 was associated with a similar fragment of the rice Xa21-resistance gene called PCR250...
February 16, 2018: Planta
https://www.readbyqxmd.com/read/29453623/a-novel-suction-coagulation-integrated-probe-for-achieving-better-hemostasis-development-and-clinical-use
#12
Hidekazu Takahashi, Naotsugu Haraguchi, Junichi Nishimura, Taishi Hata, Chu Matsuda, Hirofumi Yamamoto, Tsunekazu Mizushima, Masaki Mori, Yuichiro Doki, Kiyokazu Nakajima
PURPOSES: Modern electrosurgical tools have a specific coagulation mode called "soft coagulation". However, soft coagulation has not been widely accepted for surgical operations. To optimize the soft coagulation environment, we developed a novel suction device integrated with an electrosurgical probe, called the "Suction ball coagulator" (SBC). In this study, we aimed to optimize the SBC design with a prototyping process involving a bench test and preclinical study; then, we aimed to demonstrate the feasibility, safety, and potential effectiveness of the SBC for laparoscopic surgery in clinical settings...
February 17, 2018: Surgery Today
https://www.readbyqxmd.com/read/29453573/gene-silencing-of-human-sertoli-cells-utilizing-small-interfering-rnas
#13
Hong Wang, Qingqing Yuan, Minghui Niu, Liping Wen, Hongyong Fu, Fan Zhou, Weihui Zhang, Zuping He
Sertoli cells, as the unique somatic cells within the seminiferous tubules, play essential roles in regulating normal spermatogenesis. In addition, recent studies have demonstrated that Sertoli cells could have significant applications in regenerative medicine due to their great plasticity. However, the roles of genes in controlling the fate determinations of human Sertoli cells remain largely unknown. Silencing genes of human Sertoli cells utilizing small interfering RNAs (siRNAs) is an important method to explore their functions and mechanisms in human Sertoli cells...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29453460/development-and-initial-characterization-of-a-novel-ghrelin-receptor-crispr-cas9-knockout-wistar-rat-model
#14
L J Zallar, B J Tunstall, C T Richie, Y J Zhang, Z B You, E L Gardner, M Heilig, J Pickel, G F Koob, L F Vendruscolo, B K Harvey, L Leggio
BACKGROUND/OBJECTIVES: Ghrelin, a stomach-derived hormone implicated in numerous behaviors including feeding, reward, stress, and addictive behaviors, acts by binding to the growth hormone secretagogue receptor (GHSR). Here, we present the development, verification, and initial characterization of a novel GHSR knockout (KO) Wistar rat model created with CRISPR genome editing. METHODS: Using CRISPR/Cas9, we developed a GHSR KO in a Wistar background. Loss of GHSR mRNA expression was histologically verified using RNAscope in wild-type (WT; n = 2) and KO (n = 2) rats...
January 30, 2018: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/29453407/neovasculature-can-be-induced-by-patching-an-arterial-graft-into-a-vein-a-novel-in-vivo-model-of-spontaneous-arteriovenous-fistula-formation
#15
Yukinobu Ito, Makoto Yoshida, Daichi Maeda, Masato Takahashi, Hiroshi Nanjo, Hirotake Masuda, Akiteru Goto
Arteriovenous malformations consist of tangles of arteries and veins that are often connected by a fistula. The causes and mechanisms of these clinical entities are not fully understood. We discovered that suturing an arterial patch into the common jugular vein of rabbits led to spontaneous neovascularization, the formation of an arteriovenous fistula and the development of an arteriovenous shunt. An arterial patch excised from the common carotid artery was sutured into the common jugular vein. Within a month, a dense nidus-like neovasculature formed around the patch...
February 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29453262/insights-into-the-fundamental-physiology-of-the-uncultured-fe-oxidizing-bacterium-leptothrix-ochracea
#16
E J Fleming, T Woyke, A R Donatello, M M M Kuypers, A Sczyrba, S Littmann, D Emerson
Leptothrix ochracea is known for producing large volumes of iron-oxyhydroxide sheaths that alter wetland biogeochemistry. For over a century, these delicate structures have fascinated microbiologists and geoscientists. Because L. ochracea still resists long-term in vitro culture, the debate regarding its metabolic classification dates back to 1885. We developed a novel culturing technique for L. ochracea using in situ natural waters, and coupled this with single cell genomics and nanoscale secondary ion mass spectrophotometry (nanoSIMS) to probe L...
February 16, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29453130/charting-the-development-of-cognitive-mapping
#17
Alina Nazareth, Steven M Weisberg, Katherine Margulis, Nora S Newcombe
Developmental research beginning in the 1970s has suggested that children's ability to form cognitive maps reaches adult levels during early adolescence. However, this research has used a variety of testing procedures, often in real-world environments, which have been difficult to share widely across labs and to use to probe components of mapping, individual differences in success, and possible mechanisms of development and reasons for individual variation. In this study, we charted the development of cognitive mapping using a virtual navigation paradigm, Silcton, that allows for testing samples of substantial size in a uniform way and in which adults show marked individual differences in the formation of accurate route representations and/or in route integration...
February 13, 2018: Journal of Experimental Child Psychology
https://www.readbyqxmd.com/read/29453096/design-of-mitochondria-targeted-colorimetric-and-ratiometric-fluorescent-probes-for-rapid-detection-of-so-2-derivatives-in-living-cells
#18
Yutao Yang, Tingting Zhou, Bozan Bai, Caixia Yin, Wenzhi Xu, Wei Li
Two mitochondria-targeted colorimetric and ratiometric fluorescent probes for SO 2 derivatives were constructed based on the SO 2 derivatives-triggered Michael addition reaction. The probes exhibit high specificity toward HSO 3 - /SO 3 2- by interrupting their conjugation system resulting in a large ratiometric blue shift of 46-121nm in their emission spectrum. The two well-resolved emission bands can ensure accurate detection of HSO 3 - . The detection limits were calculated to be 1.09 and 1.35μM. Importantly, probe 1 and probe 2 were successfully used to fluorescence ratiometric imaging of endogenous HSO 3 - in BT-474 cells...
February 13, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29452883/thiocarbonyl-bound-metallonitrosyl-complexes-with-visible-light-induced-dna-cleavage-and-promising-vasodilation-activity
#19
Carlos D S Silva, Iury A Paz, Felipe D Abreu, Aurideia P de Sousa, Carla P Veríssimo, Nilberto R F Nascimento, Tércio F Paulo, Davila Zampieri, Marcos N Eberlin, Ana C S Gondim, Loraine C Andrade, Idalina M M Carvalho, Eduardo H S Sousa, Luiz G F Lopes
Nitric oxide has been involved in many key biological processes such as vasodilation, platelet aggregation, apoptosis, memory function, and this has drawn attention to the development of exogenous NO donors. Metallonitrosyl complexes are an important class of these compounds. Here, two new ruthenium nitrosyl complexes containing a thiocarbonyl ligand, with the formula cis-[Ru(phen) 2 (L)(NO)](PF 6 ) 3 (phen = phenantroline, L = thiourea or thiobenzamide), were synthesized and characterized by electronic spectroscopy, FTIR, NMR , mass spectrometry and voltammetric techniques...
February 9, 2018: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/29452651/melatonin-as-an-adjunct-to-autogenous-bone-grafts-may-result-in-small-benefits-in-probing-depth-marginal-bone-loss-and-gingival-index-of-sites-with-immediate-implants
#20
Romina Brignardello-Petersen
No abstract text is available yet for this article.
February 9, 2018: Journal of the American Dental Association
keyword
keyword
19988
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"