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Collagen VI

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https://www.readbyqxmd.com/read/27885904/the-effect-of-p2y12-inhibition-on-platelet-activation-assessed-with-aggregation-and-flow-cytometry-based-assays
#1
Tesse C Leunissen, Peter Paul Wisman, Thijs C van Holten, Philip G de Groot, Suzanne J Korporaal, Arnold C Koekman, Frans L Moll, Martin Teraa, Marianne C Verhaar, Gert Jan de Borst, Rolf T Urbanus, Mark Roest
Patients on P2Y12 inhibitors may still develop thrombosis or bleeding complications. Tailored antiplatelet therapy, based on platelet reactivity testing, might reduce these complications. Several tests have been used, but failed to show a benefit of tailored antiplatelet therapy. This could be due to the narrowness of current platelet reactivity tests, which are limited to analysis of platelet aggregation after stimulation of the adenosine diphosphate (ADP)-pathway. However, the response to ADP does not necessarily reflect the effect of P2Y12 inhibition on platelet function in vivo...
November 25, 2016: Platelets
https://www.readbyqxmd.com/read/27884442/the-induction-of-the-collagen-capsule-synthesis-by-trichinella-spiralis-is-closely-related-to-protease-activated-receptor-2
#2
Mi Kyung Park, Min Kyoung Cho, Shin Ae Kang, Bo Young Kim, Hak Sun Yu
The muscle-stage larvae of the parasite Trichinella spiralis have the ability to survive within host muscle tissue by virtue of the formation a nurse cell-parasite complex, which is surrounded by collagen. The formation of the complex is initiated by excretory-secretory (ES) proteins produced by the parasite. To determine the mechanisms underlying collagen capsule formation, we investigated the expression levels of several types of collagen genes and TGF-βI signaling-related genes (Smad2 and Smad3) in muscle cells...
October 30, 2016: Veterinary Parasitology
https://www.readbyqxmd.com/read/27870875/a-novel-organ-culture-model-to-quantify-collagen-remodeling-in-tree-shrew-sclera
#3
Sarah Baldivia, Alexander Levy, Shylaja Hegde, Stijn J A Aper, Maarten Merkx, Rafael Grytz
Increasing evidence suggests that unknown collagen remodeling mechanisms in the sclera underlie myopia development. We are proposing a novel organ culture system in combination with two-photon fluorescence imaging to quantify collagen remodeling at the tissue- and lamella-level. Tree shrew scleral shells were cultured up to 7 days in serum-free media and cellular viability was investigated under: (i) minimal tissue manipulations; (ii) removal of intraocular tissues; gluing the eye to a washer using (iii) 50 μL and (iv) 200 μL of cyanoacrylate adhesive; (v) supplementing media with Ham's F-12 Nutrient Mixture; and (vi) culturing eyes subjected to 15 mmHg intraocular pressure in our new bioreactor...
2016: PloS One
https://www.readbyqxmd.com/read/27854213/bethlem-myopathy-phenotypes-and-follow-up-description-of-8-patients-at-the-mildest-end-of-the-spectrum
#4
Simao Cruz, Sebastian Figueroa-Bonaparte, Eduard Gallardo, Alix de Becdelièvre, Corine Gartioux, Valérie Allamand, Patricia Piñol, Maria Angels Rodriguez Garcia, Cecilia Jiménez-Mallebriera, Jaume Llauger, Lidia González-Rodríguez, Elena Cortes-Vicente, Isabel Illa, Jordi Díaz-Manera
The classical phenotypes of collagen VI-associated myopathies are well described. Little is known, however, about the progression of patients at the mildest end of the clinical spectrum. In this report, we describe the clinical findings and the results of MRI, muscle biopsy, collagen VI expression in cultured skin fibroblasts and genetic tests of a series of patients with Bethlem myopathy. Our series highlights the existence of mild presentations of this disorder that progresses only slightly and can easily be overlooked...
May 27, 2016: Journal of Neuromuscular Diseases
https://www.readbyqxmd.com/read/27853331/effects-of-platelet-rich-plasma-platelet-rich-fibrin-with-and-without-stromal-cell-derived-factor-1-on-repairing-full-thickness-cartilage-defects-in-knees-of-rabbits
#5
Soghra Bahmanpour, Maryam Ghasemi, Mohsen Sadeghi-Naini, Iraj Ragerdi Kashani
BACKGROUND: The purpose of this study was to create biomaterial scaffolds like platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) containing stromal cell-derived factor-1 (SDF1) as a chemokine to induce hyaline cartilage regeneration of rabbit knee in a full thickness defect. METHODS: We created a full thickness defect in the trochlear groove of thirty-six bilateral knees of eighteen mature male rabbits. The knees were randomly divided into six groups (group I: untreated control, group II: PRP, group III: PRF, group IV: Gelatin+SDF1, group V: PRP+SDF1, and group VI: PRF+SDF1)...
November 2016: Iranian Journal of Medical Sciences
https://www.readbyqxmd.com/read/27818280/gpvi-and-thromboxane-receptor-on-platelets-promote-proinflammatory-macrophage-phenotypes-during-cutaneous-inflammation
#6
Sandra Pierre, Bona Linke, Suo Jing, Neda Tarighi, Domenico Del Turco, Dominique Thomas, Nerea Ferreiros, David Stegner, Stefanie Frölich, Marco Sisignano, Sascha Meyer Dos Santos, Natasja deBruin, Rolf M Nüsing, Thomas Deller, Bernhard Nieswandt, Gerd Geisslinger, Klaus Scholich
Platelets are well known for their role in hemostasis but are also increasingly recognized for their supporting role in innate immune responses. Here, we studied the role of platelets in the development of peripheral inflammation and found that platelets colocalize with macrophages in the inflamed tissue outside of blood vessels in different animal models for cutaneous inflammation. In macrophages isolated from paws during zymosan-induced inflammation treatment with collagen-induced thromboxane synthesis through the platelet-expressed collagen receptor glycoprotein VI (GPVI)...
November 3, 2016: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/27811845/dental-pulp-stem-cells-express-tendon-markers-under-mechanical-loading-and-are-a-potential-cell-source-for-tissue-engineering-of-tendon-like-tissue
#7
Yu-Ying Chen, Sheng-Teng He, Fu-Hua Yan, Peng-Fei Zhou, Kai Luo, Yan-Ding Zhang, Yin Xiao, Min-Kui Lin
Postnatal mesenchymal stem cells have the capacity to differentiate into multiple cell lineages. This study explored the possibility of dental pulp stem cells (DPSCs) for potential application in tendon tissue engineering. The expression of tendon-related markers such as scleraxis, tenascin-C, tenomodulin, eye absent homologue 2, collagens I and VI was detected in dental pulp tissue. Interestingly, under mechanical stimulation, these tendon-related markers were significantly enhanced when DPSCs were seeded in aligned polyglycolic acid (PGA) fibre scaffolds...
November 4, 2016: International Journal of Oral Science
https://www.readbyqxmd.com/read/27791138/laminin-targeting-of-a-peripheral-nerve-highlighting-peptide-enables-degenerated-nerve-visualization
#8
Heather L Glasgow, Michael A Whitney, Larry A Gross, Beth Friedman, Stephen R Adams, Jessica L Crisp, Timon Hussain, Andreas P Frei, Karel Novy, Bernd Wollscheid, Quyen T Nguyen, Roger Y Tsien
Target-blind activity-based screening of molecular libraries is often used to develop first-generation compounds, but subsequent target identification is rate-limiting to developing improved agents with higher specific affinity and lower off-target binding. A fluorescently labeled nerve-binding peptide, NP41, selected by phage display, highlights peripheral nerves in vivo. Nerve highlighting has the potential to improve surgical outcomes by facilitating intraoperative nerve identification, reducing accidental nerve transection, and facilitating repair of damaged nerves...
October 20, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27781307/composition-and-adaptation-of-human-myotendinous-junction-and-neighboring-muscle-fibers-to-heavy-resistance-training
#9
J R Jakobsen, A L Mackey, A B Knudsen, M Koch, M Kjaer, M R Krogsgaard
The myotendinous junction (MTJ) is a common site of strain injury and yet understanding of its composition and ability to adapt to loading is poor. The main aims of this study were to determine the profile of selected collagens and macrophage density in human MTJ and adjoining muscle fibers, and to investigate whether heavy exercise loading would alter this profile. Fifteen individuals scheduled for anterior cruciate ligament repair surgery were randomized into three groups: control, acute or 4 weeks heavy resistance training...
October 26, 2016: Scandinavian Journal of Medicine & Science in Sports
https://www.readbyqxmd.com/read/27774697/livedoid-vasculopathy-and-popliteal-artery-occlusion-in-a-patient-with-protein-s-deficiency
#10
Takayuki Nakayama, Kentaro Mizutani, Ichiro Hanamura, Hidefumi Kato, Akiyoshi Takami, Kyosuke Takeshita, Daisuke Watanabe
Livedoid vasculopathy (LV) is a chronic disease with recurrent reticularis and ulcers, mainly affecting the feet and lower legs. The pathogenesis of LV has not been yet thoroughly understood, but thrombosis is thought to play a major role because fibrin deposition within both the wall and lumen of affected vessels is pathologically detected. A 68-year-old woman first presented to our hospital in 2004 with a 6-year history of a reticular rash and ulceration on the lower legs. Screening tests for vasculitis and collagen disease were mostly normal, leading to diagnosis of LV...
October 24, 2016: Journal of Dermatology
https://www.readbyqxmd.com/read/27766315/cross-linking-gpvi-fc-by-anti-fc-antibodies-potentiates-its-inhibition-of%C3%A2-atherosclerotic-plaque-and-collagen-induced-platelet-activation
#11
Janina Jamasbi, Remco T A Megens, Mariaelvy Bianchini, Kerstin Uhland, Götz Münch, Martin Ungerer, Shachar Sherman, Alexander Faussner, Richard Brandl, Christine John, Johannes Buchner, Christian Weber, Reinhard Lorenz, Natalie Elia, Wolfgang Siess
To enhance the antithrombotic properties of recombinant glycoprotein VI fragment crystallizable (GPVI-Fc), the authors incubated GPVI-Fc with anti-human Fc antibodies to cross-link the Fc tails of GPVI-Fc. Cross-linking potentiated the inhibition of human plaque- and collagen-induced platelet aggregation by GPVI-Fc under static and flow conditions without increasing bleeding time in vitro. Cross-linking with anti-human-Fc Fab2 was even superior to anti-human-Fc immunoglobulin G (IgG). Advanced optical imaging revealed a continuous sheath-like coverage of collagen fibers by cross-linked GPVI-Fc complexes...
April 2016: JACC. Basic to Translational Science
https://www.readbyqxmd.com/read/27717959/endotrophin-a-multifaceted-player-in-metabolic-dysregulation-and-cancer-progression-is-a-predictive-biomarker-for-the-response-to-ppar%C3%AE-agonist-treatment
#12
EDITORIAL
Kai Sun, Jiyoung Park, Min Kim, Philipp E Scherer
Endotrophin is a cleavage product derived from the collagen VI(α3) chain. Collagen VI is expressed in a number of different tissues, but adipose tissue is a particularly prominent source for this extracellular matrix constituent. Mice lacking collagen VI are metabolically healthier due to reduced fibrosis in adipose tissue. Endotrophin seems to be one of the key players of collagen VI-mediated signalling effects, including its pro-fibrotic nature and chemoattractant properties for macrophages, while also playing an important role in cancer progression and the chemoresistance of tumour cells...
January 2017: Diabetologia
https://www.readbyqxmd.com/read/27716343/high-levels-of-biomarkers-of-collagen-remodeling-are-associated-with-increased-mortality-in-copd-results-from-the-eclipse-study
#13
Jannie M B Sand, Diana J Leeming, Inger Byrjalsen, Asger R Bihlet, Peter Lange, Ruth Tal-Singer, Bruce E Miller, Morten A Karsdal, Jørgen Vestbo
BACKGROUND: There is a need to identify individuals with COPD at risk for disease progression and mortality. Lung tissue remodeling is associated with the release of extracellular matrix (ECM) fragments into the peripheral circulation. We hypothesized that ECM remodeling was associated with mortality in COPD and measured neo-epitopes originating from ECM proteins associated with lung tissue remodeling. METHODS: Biomarkers of ECM remodeling were assessed in a subpopulation (n = 1000) of the Evaluation of COPD Longitudinally to Identify Predictive Surrogate End-points (ECLIPSE) cohort...
October 4, 2016: Respiratory Research
https://www.readbyqxmd.com/read/27697834/tumor-macrophages-are-pivotal-constructors-of-tumor-collagenous-matrix
#14
Ran Afik, Ehud Zigmond, Milena Vugman, Mordehay Klepfish, Elee Shimshoni, Metsada Pasmanik-Chor, Anjana Shenoy, Elad Bassat, Zamir Halpern, Tamar Geiger, Irit Sagi, Chen Varol
Tumor-associated macrophages (TAMs) promote tumor development, invasion, and dissemination by various mechanisms. In this study, using an orthotopic colorectal cancer (CRC) model, we found that monocyte-derived TAMs advance tumor development by the remodeling of its extracellular matrix (ECM) composition and structure. Unbiased transcriptomic and proteomic analyses of (a) TAM-abundant and -deficient tumor tissues and (b) sorted tumor-associated and -resident colonic macrophage subpopulations defined a distinct TAM-induced ECM molecular signature composed of an ensemble of matricellular proteins and remodeling enzymes they provide to the tumor microenvironment...
October 17, 2016: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/27696451/fibrosis-is-not-just-fibrosis-basement-membrane-modelling-and-collagen-metabolism-differs-between-hepatitis-b-and-c-induced-injury
#15
M J Nielsen, M A Karsdal, K Kazankov, H Grønbaek, A Krag, D J Leeming, D Schuppan, J George
BACKGROUND: While morphological patterns differ, the molecular phenotype of liver fibrosis is considered a stereotypical response to chronic liver injury. However, with different cellular triggers and networks regulating fibrosis, the molecular responses of the injured liver may not be identical. AIM: To investigate whether differences in extracellular matrix (ECM) composition of the liver during fibrogenesis in two seemingly similar types of viral hepatitis could be reflected by differences in ECM turnover...
December 2016: Alimentary Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/27687726/psoralen-and-ultraviolet-a-light-treatment-directly-affects-phosphatidylinositol-3-kinase-signal-transduction-by-altering-plasma-membrane-packing
#16
Britt Van Aelst, Rosalie Devloo, Pierre Zachée, Ruben t'Kindt, Koen Sandra, Philippe Vandekerckhove, Veerle Compernolle, Hendrik B Feys
Psoralen and ultraviolet A light (PUVA) are used to kill pathogens in blood products and as a treatment of aberrant cell proliferation in dermatitis, cutaneous T-cell lymphoma, and graft-versus-host disease. DNA damage is well described, but the direct effects of PUVA on cell signal transduction are poorly understood. Because platelets are anucleate and contain archetypal signal transduction machinery, they are ideally suited to address this. Lipidomics on platelet membrane extracts showed that psoralen forms adducts with unsaturated carbon bonds of fatty acyls in all major phospholipid classes after PUVA...
November 18, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27681457/altered-collagen-turnover-in-factor-viii-deficient-rats-with-hemophilic-arthropathy-identifies-potential-novel-serological-biomarkers-in-hemophilia
#17
T Manon-Jensen, M A Karsdal, L N Nielsen, M Kjelgaard-Hansen, B Vandahl, E H N Olsen, M Enoksson, K Roepstorff
: Essentials Joint bleeding in hemophilia may induce significant remodeling of the extracellular matrix. Biomarkers of collagen turnover were investigated in a F8(-/-) rat model of hemophilic arthropathy. Biomarkers of cartilage degradation increased significantly during development of arthropathy. Basement membrane and interstitial matrix turnover changed significantly following hemarthrosis. SUMMARY: Background Hemophilic arthropathy is a severe complication of hemophilia...
September 28, 2016: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/27672655/aryl-hydrocarbon-receptor-activation-by-tcdd-modulates-expression-of-extracellular-matrix-remodeling-genes-during-experimental-liver-fibrosis
#18
Cheri L Lamb, Giovan N Cholico, Daniel E Perkins, Michael T Fewkes, Julia Thom Oxford, Trevor J Lujan, Erica E Morrill, Kristen A Mitchell
The aryl hydrocarbon receptor (AhR) is a soluble, ligand-activated transcription factor that mediates the toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Increasing evidence implicates the AhR in regulating extracellular matrix (ECM) homeostasis. We recently reported that TCDD increased necroinflammation and myofibroblast activation during liver injury elicited by carbon tetrachloride (CCl4). However, TCDD did not increase collagen deposition or exacerbate fibrosis in CCl4-treated mice, which raises the possibility that TCDD may enhance ECM turnover...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27665127/global-and-targeted-proteomics-of-prostate-cancer-cell-secretome-combination-of-2-dimensional-image-converted-analysis-of-liquid-chromatography-and-mass-spectrometry-and-in-silico-selection-selected-reaction-monitoring-analysis
#19
Armania Nurdin, Yutaro Hoshi, Toshihiro Yoneyama, Eisuke Miyauchi, Masanori Tachikawa, Michitoshi Watanabe, Tetsuya Terasaki
Prostate-specific antigen is currently the only protein biomarker routinely used as a diagnostic tool for early detection and treatment monitoring of prostate cancer. However, it remains questionable whether prostate-specific antigen-based screening can sensitively and selectively identify the presence and progression status of primary and metastatic prostate cancers. Hence, the purpose of this study was to identify potential biomarker candidates in the secretome of primary and metastatic prostate cancer cells by using a combination of global and targeted proteomics...
November 2016: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/27660222/expression-of-peroxiredoxins-and-pulmonary-surfactant-protein-a-induced-by-silica-in-rat-lung-tissue
#20
Nan Liu, Ling Xue, Yi Guan, Qing Zhao Li, Fu Yuan Cao, Shu Lan Pang, Wei Jun Guan
Silicosis is one of the most serious occupational diseases in China and dates back to centuries ago. In this study, we successfully established a rat model of silicosis by intratracheal silica injection for 28 days and determined hydroxyproline levels to evaluate collagen metabolism in lung homogenates. Oxidative stress status was evaluated by detecting catalase and glutathione peroxidase activities. Expression levels of peroxiredoxins (Prx I and Prx VI) were detected by Western blotting. Pulmonary surfactant protein A (SP-A) levels in rat serum and lung tissue were analyzed by ELISA, and SP-A and Prx expression levels in lung tissues were detected by immunohistochemistry...
August 2016: Biomedical and Environmental Sciences: BES
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