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https://www.readbyqxmd.com/read/29452112/molecular-diagnosis-of-protozoan-parasites-by-recombinase-polymerase-amplification
#1
A Castellanos-Gonzalez, A C White, P Melby, B Travi
Infections caused by protozoan parasites affect millions of people around the world. Traditionally, diagnosis was made by microscopy, which is insensitive and in some cases not specific. Molecular methods are highly sensitive and specific, but equipment costs and personnel training limit its availability only to specialized centers, usually far from populations with the highest risk of infection. Inexpensive methods that can be applied at the point of care (POC), especially in places with limited health infrastructure, would be a major advantage...
February 13, 2018: Acta Tropica
https://www.readbyqxmd.com/read/29452105/label-free-electrochemical-aptasensor-for-detection-of-alpha-fetoprotein-based-on-afp-aptamer-and-thionin-reduced-graphene-oxide-gold-nanoparticles
#2
Guiyin Li, Shanshan Li, Zhihong Wang, Yewei Xue, Chenyang Dong, Junxiang Zeng, Yong Huang, Jintao Liang, Zhide Zhou
Sensitive and accurate detection of tumor markers is critical to early diagnosis, point-of-care and portable medical supervision. Alpha fetoprotein (AFP) is an important clinical tumor marker for hepatocellular carcinoma (HCC), and the concentration of AFP in human serum is related to the stage of HCC. In this paper, a label-free electrochemical aptasensor for AFP detection was fabricated using AFP-aptamer as the recognition molecule and thionin/reduced graphene oxide/gold nanoparticles (TH/RGO/Au NPs) as the sensor platform...
February 13, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/29451781/multiple-snps-detection-based-on-lateral-flow-assay-for-phenylketonuria-diagnostic
#3
Xiaonan Liu, Chao Zhang, Kewu Liu, Han Wang, Chaoxia Lu, Hang Li, Kai Hua, Juanli Zhu, Wenli Hui, Yali Cui, Xue Zhang
Single nucleotide polymorphisms (SNPs) are closely related to genetic diseases, but current SNP detection methods, such as DNA microarrays that include tedious procedures and expensive, sophisticated instruments, are unable to perform rapid SNPs detection in clinical practice, especially for those multiple SNPs related to genetic diseases. In this study, we report a sensitive, low cost, and easy-to-use point-of-care testing (POCT) system formed by combining amplification refractory mutation system (ARMS) polymerase chain reaction with gold magnetic nanoparticles (GMNPs) and lateral flow assay (LFA) noted as the ARMS-LFA system, which allow us to use a uniform condition for multiple SNPs detection simultaneously...
February 16, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29451280/a-remote-computing-based-point-of-care-colorimetric-detection-system-with-a-smartphone-under-complex-ambient-light-conditions
#4
Xu Bao, Shu Jiang, Yun Wang, Miao Yu, Juan Han
Smartphone-based colorimetric detection has been one of the most commonly used techniques for point-of-care detection in recent years. However, there are two defects in the current detection system. One is the need of a light-tight box to isolate the impact of ambient light, and the other is the increased calculation with the number of probes. In this paper, a colorimetric detection system was coupled with a new color calibration method for detection under complex ambient light conditions. A 3 × 4 colorimetric probe array was used to display the color changes of different analytes...
February 16, 2018: Analyst
https://www.readbyqxmd.com/read/29451114/molecular-tools-to-study-preharvest-food-safety-challenges
#5
Deepak Kumar, Siddhartha Thakur
Preharvest food safety research and activities have advanced over time with the recognition of the importance and complicated nature of the preharvest phase of food production. In developed nations, implementation of preharvest food safety procedures along with strict monitoring and containment at various postharvest stages such as slaughter, processing, storage, and distribution have remarkably reduced the burden of foodborne pathogens in humans. Early detection and adequate surveillance of pathogens at the preharvest stage is of the utmost importance to ensure a safe meat supply...
February 2018: Microbiology Spectrum
https://www.readbyqxmd.com/read/29451066/psychometric-properties-of-mdhaq-rapid3-in-patients-with-systemic-lupus-erythematosus
#6
N Annapureddy, D Giangreco, H Devilliers, J A Block, M Jolly
Background The Multidimensional Health Assessment Questionnaire (MDHAQ) is a patient-reported outcome (PRO) tool that includes the Routine Assessment of Patient Index Data 3 (RAPID3), an index that can be calculated at the point of care. The objective of this study was to perform psychometric analyses of MDHAQ/RAPID3 to study its measurement properties in systemic lupus erythematosus (SLE). Methods The MDHAQ was completed by 161 SLE patients in routine care, along with LupusPRO (a disease-specific PRO). The SLE disease-specific activity index (SELENA-SLEDAI) and damage (SDI) were assessed...
January 1, 2018: Lupus
https://www.readbyqxmd.com/read/29450975/tropoelastin-implants-that-accelerate-wound-repair
#7
Suzanne M Mithieux, Behnaz Aghaei-Ghareh-Bolagh, Leping Yan, Kekini V Kuppan, Yiwei Wang, Francia Garces-Suarez, Zhe Li, Peter K Maitz, Elizabeth A Carter, Christina Limantoro, Wojciech Chrzanowski, David Cookson, Alan Riboldi-Tunnicliffe, Clair Baldock, Kosuke Ohgo, Kristin K Kumashiro, Glenn Edwards, Anthony S Weiss
A novel, pure, synthetic material is presented that promotes the repair of full-thickness skin wounds. The active component is tropoelastin and leverages its ability to promote new blood vessel formation and its cell recruiting properties to accelerate wound repair. Key to the technology is the use of a novel heat-based, stabilized form of human tropoelastin which allows for tunable resorption. This implantable material contributes a tailored insert that can be shaped to the wound bed, where it hydrates to form a conformable protein hydrogel...
February 16, 2018: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29450691/financial-impact-of-inaccurate-adverse-event-recording-post-hip-fracture-surgery-addendum-to-adverse-event-recording-post-hip-fracture-surgery
#8
Matthew J Lee, Kevin Doody, Khalid M S Mohamed, Audrey Butler, John Street, Brian Lenehan
INTRODUCTION: A study in 2011 by (Doody et al. Ir Med J 106(10):300-302, 2013) looked at comparing inpatient adverse events recorded prospectively at the point of care, with adverse events recorded by the national Hospital In-Patient Enquiry (HIPE) System. METHODS: In the study, a single-centre University Hospital in Ireland treating acute hip fractures in an orthopaedic unit recorded 39 patients over a 2-month (August-September 2011) period, with 55 adverse events recorded prospectively in contrast to the HIPE record of 13 (23...
February 15, 2018: Irish Journal of Medical Science
https://www.readbyqxmd.com/read/29448635/surface-plasmon-resonance-aptamer-biosensor-for-discriminating-pathogenic-bacteria-vibrio-parahaemolyticus
#9
Ji-Young Ahn, Kyeong-Ah Lee, Moon-Jong Lee, Simranjeet Singh Sekhon, Sung-Keun Rhee, Sung-Jin Cho, Jung Ho Ko, Lyon Lee, Janet Han, Sang Yong Kim, Jiho Min, Yang-Hoon Kim
In this paper, whole-bacteria SELEX (WB-SELEX) strategy was adopted to isolate specific aptamers against Vibrio parahaemolyticus. Round selection for V. parahaemolyticus was conducted 11 rounds, including two negative selection rounds. It was determined through real-time PCR amplification and post-SELEX experiment. The selected aptmers had high binding property and specificity to V. parahaemolyticus. Of 28 aptamers tested, VPCA-apta#1 had the highest binding affinity compared to other aptamer candidates obtained...
March 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29447855/rpa-using-a-multiplexed-cartridge-for-low-cost-point-of-care-diagnostics-in-the-field
#10
Luck Tosan Ereku, Ruth E Mackay, Pascal Craw, Angel Naveenathayalan, Thomas Stead, Manorharanehru Branavan, Wamadeva Balachandran
A point of care device utilising Lab-on-a-Chip technologies that is applicable for biological pathogens was designed, fabricated and tested showing sample in to answer out capabilities. The purpose of the design was to develop a cartridge with the capability to perform nucleic acid extraction and purification from a sample using a chitosan membrane at an acidic pH. Waste was stored within the cartridge with the use of sodium polyacrylate to solidify or gelate the sample in a single chamber. Nucleic acid elution was conducted using the RPA amplification reagents (alkaline pH)...
February 12, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/29446920/homogeneous-noncompetitive-luminescent-immunodetection-of-small-molecules-by-ternary-protein-fragment-complementation
#11
Yuki Ohmuro-Matsuyama, Hiroshi Ueda
The homogeneous immunological detection of small molecules at high sensitivity is still a daunting task. Here, we tried sensitive noncompetitive detection of small peptides based on the open-sandwich immunoassay principle, which was combined with a bioluminescent protein-fragment complementation assay (PCA) in vitro. Since the detection of antigen-induced approximation of the two antibody variable region fragments V H and V L by the standard Nanoluc-based PCA utilizing larger (LgBiT) and shorter (SmBiT) fragments was not successful, we decided to further split LgBiT into two, yielding smaller N-terminal derivative (LnBiT) and two C-terminal, 11 residue peptides (LcBiT and SmBiT) corresponding to consecutive beta strands, to which V H and V L were each fused and expressed in Escherichia coli cells...
February 15, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29445833/functionalized-au-ag-au-nanoparticles-as-an-optical-and-sers-dual-probe-for-lateral-flow-sensing
#12
Tingting Bai, Meng Wang, Min Cao, Juan Zhang, Kangzhen Zhang, Ping Zhou, Zhengxia Liu, Ying Liu, Zhirui Guo, Xiang Lu
Lateral flow assay strips (LFASs) with Au nanoparticles (NPs) have been widely used as a probe for biomarkers in point-of-care testing; however, there still remain challenges in detection sensitivity and quantitative analysis. In this study, we developed a surface-enhanced Raman scattering (SERS)-based LFAS for quantitative analysis of a biomarker in the low concentration range. Moreover, apart from conventional Au NPs, three other types of citrate-capped Au-Ag bimetallic NPs: Au core with Ag shell NPs (Au@Ag NPs), rattle-like Au core in Ag-Au shell NPs (Au@Ag-Au NPs) and Ag-Au NPs were prepared and functionalized, and their solution-based SERS activities were comprehensively studied by experimental measurement and theoretical analysis...
February 14, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29445802/pulsatile-plasma-filtration-and-cell-free-dna-amplification-using-a-water-head-driven-point-of-care-testing-chip
#13
Yonghun Lee, Dong-Min Kim, Zhenglin Li, Dong-Eun Kim, Sung-Jin Kim
We demonstrate a microfiltration chip that separates blood plasma by using water-head-driven pulsatile pressures rather than any external equipment and use it for on-chip amplification of nucleic acids. The chip generates pulsatile pressures to significantly reduce filter clogging without hemolysis, and consists of an oscillator, a plasma-extraction pump, and filter units. The oscillator autonomously converts constant water-head pressure to pulsatile pressure, and the pump uses the pulsatile pressure to extract plasma through the filter...
February 15, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29445800/acoustic-separation-in-plastic-microfluidics-for-rapid-detection-of-bacteria-in-blood-using-engineered-bacteriophage
#14
P Dow, K Kotz, S Gruszka, J Holder, J Fiering
A more effective treatment of bacteremia requires a diagnostic platform that is both sensitive, accurate and rapid. Currently, clinical laboratory techniques require growth of bacteria prior to diagnosis, take days to complete, and leave empiric therapy and broad spectrum antibiotics as the only option at the onset of treatment. In order to bypass this growth requirement, we engineered a system that purifies bacteria from blood to improve performance in a bacteriophage-based luminescence assay. To perform the purification, we used acoustophoresis in plastic microfluidic chips, enabling future development into a low cost point-of-care system...
February 15, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29445475/endogenous-carbon-monoxide-production-in-extracorporeal-membrane-oxygenation-related-hemolysis-potential-use-of-point-of-care-co-oximetry-carboxyhemoglobin-to-detect-hemolysis
#15
Chan Kai Man, Lam Koon Ngai
Extracorporeal membrane oxygenation (ECMO)-related hemolysis is a rare but devastating condition. Death is inevitable without early recognition and prompt management. Endogenous carbon monoxide production, as an epiphenomenon of hemolysis, potentially allows rapid detection of such condition by use of point-of-care CO-oximetry carboxyhemoglobin.
February 2018: Clinical Case Reports
https://www.readbyqxmd.com/read/29444563/recent-update-on-coagulation-management-and-hemostatic-therapies-in-liver-transplantation
#16
Ezeldeen Abuelkasem, Kenichi A Tanaka, Raymond M Planinsic
Liver transplantation is an ever-evolving field and understanding coagulation management and hemostatic therapies is essential for good outcomes. In this review, we discuss current monitoring tools available in the operating room and the hemostatic agents available to control hemostasis. Multifactorial coagulation defects are discussed and point-of care monitoring is reviewed to guide selection of hemostatic agents when indicated.
February 14, 2018: Minerva Anestesiologica
https://www.readbyqxmd.com/read/29443503/dual-color-bioluminescent-sensor-proteins-for-therapeutic-drug-monitoring-of-anti-tumor-antibodies
#17
Martijn van Rosmalen, Yan Ni, Daan F M Vervoort, Remco Arts, Susann Katrina Julie Ludwig, Maarten Merkx
Monitoring the levels of therapeutic antibodies in individual patients would allow patient-specific dose optimization, with the potential for major therapeutic and financial benefits. Our group recently developed a new platform of bioluminescent sensor proteins (LUMABS; LUMinescent AntiBody Sensor) that allow antibody detection directly in blood plasma. In this study we targeted four clinically important therapeutic antibodies, the Her2-receptor targeting trastuzumab, the anti-CD20 antibodies rituximab and obinutuzumab, and the EGFR-blocking cetuximab...
February 14, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29441759/determinants-used-to-justify-the-strength-of-recommendations-among-korean-clinical-practice-guidelines
#18
Ein Soon Shin, Ji Eun Jang, Ji Yun Yeon, Da Sol Kim, Sung Goo Chang, Yoon Seong Lee
BACKGROUND: A standardized systematic approach to grade evidence and the strength of recommendations is important for guideline users to minimize bias and help interpret the most suitable decisions at the point of care. The study aims to identify and classify determinants used to make judgement for the strength of recommendations among 56 Korean clinical practice guidelines (CPGs), and explore strong recommendations based on low quality of evidence. METHODS: Determinants used in the Grading of Recommendations Assessment, Development and Evaluation (GRADE) approach among 34 CPGs which have reported both strength of recommendations and level of evidence were reviewed...
February 19, 2018: Journal of Korean Medical Science
https://www.readbyqxmd.com/read/29440656/gold-nanoparticle-based-rapid-detection-and-isolation-of-cells-using-ligand-receptor-chemistry
#19
Pradipta Ranjan Rauta, Pavan M Hallur, Aditya Chaubey
Identification and isolation of low-frequency cells of interest from a heterogeneous cell mixture is an important aspect of many diagnostic applications (including enumeration of circulating tumor cells) and is integral to various assays in (cancer) biology. Current techniques typically require expensive instrumentation and are not amenable to high throughput. Here, we demonstrate a simple and effective platform for cell detection and isolation using gold nanoparticles (Au NPs) conjugated with hyaluronic acid (HA) i...
February 13, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29440016/reducing-inappropriate-antibiotic-prescribing-for-children-in-primary-care-a-cluster-randomised-controlled-trial-of-two-interventions
#20
Marieke B Lemiengre, Jan Y Verbakel, Roos Colman, Tine De Burghgraeve, Frank Buntinx, Bert Aertgeerts, Frans De Baets, An De Sutter
BACKGROUND: Antibiotics are overprescribed for non-severe acute infections in children in primary care. AIM: To explore two different interventions that may reduce inappropriate antibiotic prescribing for non-severe acute infections. DESIGN AND SETTING: A cluster randomised, factorial controlled trial in primary care, in Flanders, Belgium. METHOD: Family physicians (FPs) enrolled children with non-severe acute infections into this study...
February 12, 2018: British Journal of General Practice: the Journal of the Royal College of General Practitioners
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