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https://www.readbyqxmd.com/read/29053912/community-based-screening-of-hepatitis-c-with-a-one-step-rna-detection-algorithm-from-dried-blood-spots-analysis-of-key-populations-in-barcelona-spain
#1
V Saludes, C Folch, A Morales-Carmona, L Ferrer, L Fernández, R Muñoz, M Jiménez, E Loureiro, P Fernández-Dávila, E Bascuñana, J Casabona, E Martró
Alternative strategies are required to enhance the diagnosis of silent hepatitis C virus (HCV) infections in hard-to-reach key populations at risk. Among them, HCV prevalence and bio-behavioural data is scarce for HIV-negative men who have sex with men (MSM) and men and trans-women sex workers. We sought to describe and assess the potential benefits of a community-based one-step HCV screening and confirmatory strategy for these populations in Barcelona. The screening strategy based on a real-time RT-PCR assay for HCV-RNA detection in dried-blood spots (DBS) was validated and implemented in addition to an antibody point-of-care test in a community centre...
October 20, 2017: Journal of Viral Hepatitis
https://www.readbyqxmd.com/read/29053610/lab-on-chip-surface-enhanced-raman-analysis-by-aerosol-jet-printing-and-roll-to-roll-hot-embossing
#2
Anne Habermehl, Noah Strobel, Ralph Eckstein, Nico Bolse, Adrian Mertens, Gerardo Hernandez-Sosa, Carsten Eschenbaum, Uli Lemmer
Surface-enhanced Raman spectroscopy (SERS) combines the high specificity of Raman scattering with high sensitivity due to an enhancement of the electromagnetic field by metallic nanostructures. However, the tyical fabrication methods of SERS substrates suffer from low throughput and therefore high costs. Furthermore, point-of-care applications require the investigation of liquid solutions and thus the integration of the SERS substrate in a microfluidic chip. We present a roll-to-roll fabrication approach for microfluidics with integrated, highly efficient, surface-enhanced Raman scattering structures...
October 20, 2017: Sensors
https://www.readbyqxmd.com/read/29052772/imaging-of-thoracic-tuberculosis-in-children-current-and-future-directions
#3
REVIEW
Kushaljit Singh Sodhi, Ashu S Bhalla, Nasreen Mahomed, Bernard F Laya
Tuberculosis continues to be an important cause of morbidity and mortality worldwide. It is the leading cause of infection-related deaths worldwide. Children are amongst the high-risk groups for developing tuberculosis and often pose a challenge to the clinicians in making a definitive diagnosis. The newly released global tuberculosis report from World Health Organization reveals a 50% increase in fatality from tuberculosis in children. Significantly, diagnostic and treatment algorithms of tuberculosis for children differ from those of adults...
September 2017: Pediatric Radiology
https://www.readbyqxmd.com/read/29048155/wavelengths-and-lifetimes-of-paper-autofluorescence-a-simple-substrate-screening-process-to-enhance-the-sensitivity-of-fluorescence-based-assays-in-paper
#4
Kamal G Shah, Paul Yager
Porous media made of nitrocellulose and glass fiber are common "paper" substrates for lateral flow assays, microfluidic paper analytical devices, and other point-of-care diagnostic assays. Such assays commonly use optical labels such as gold nanoparticles, latex beads, or fluorescent nanoparticles to visualize the presence of analytes. Fluorescent labels are commonly used in bioassays to enhance sensitivity, but autoluminescence of the paper substrate worsens signal-to-noise ratios of fluorescence-based assays...
October 19, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29046408/-smart-diagnosis-of-parasites-using-smartphones
#5
Muhammad A Saeed, Abdul Jabbar
Accurate and rapid diagnosis is crucial in combating parasitic diseases that cause millions of deaths worldwide. However, the scarcity of specialized diagnostic equipment in low-to-middle income countries is one of the barriers in effective management of parasitic diseases, which warrants the need for alternative, inexpensive and point-of-care diagnostic tools. Due to their multiple built-in sensors, smartphones offer cost-effective alternative to expensive diagnostic devices. However, the use of smartphones in parasitic diagnoses remains in its infancy...
October 18, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29045017/connecting-biology-to-electronics-molecular-communication-via-redox-modality
#6
REVIEW
Yi Liu, Jinyang Li, Tanya Tschirhart, Jessica L Terrell, Eunkyoung Kim, Chen-Yu Tsao, Deanna L Kelly, William E Bentley, Gregory F Payne
Biology and electronics are both expert at for accessing, analyzing, and responding to information. Biology uses ions, small molecules, and macromolecules to receive, analyze, store, and transmit information, whereas electronic devices receive input in the form of electromagnetic radiation, process the information using electrons, and then transmit output as electromagnetic waves. Generating the capabilities to connect biology-electronic modalities offers exciting opportunities to shape the future of biosensors, point-of-care medicine, and wearable/implantable devices...
October 18, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29043935/an-analysis-of-physician-assistant-libguides-a-tool-for-collection-development
#7
Catherine V Johnson, Scott Y Johnson
The Physician Assistant (PA) specialty encompasses many subject areas and requires many types of library resources. An analysis of PA LibGuides was performed to determine most frequently recommended resources. A sample of LibGuides from U.S. institutions accredited by the Accreditation Review Commission on Education for the Physician Assistant (ARC-PA) was included in this study. Resources presented on guides were tabulated and organized by resource type. Databases and point-of-care tools were the types of resources listed by the most LibGuides...
October 2017: Medical Reference Services Quarterly
https://www.readbyqxmd.com/read/29043325/a-fully-integrated-microfluidic-platform-for-highly-sensitive-analysis-of-immunochemical-parameters
#8
Sascha Lutz, Eloisa Lopez-Calle, Pamela Espindola, Christoph Boehm, Thorsten Brueckner, Juergen Spinke, Moritz Marcinowski, Thomas Keller, Armin Tgetgel, Nicolas Herbert, Thomas Fischer, Erik Beiersdorf
We present a novel fully integrated centrifugal microfluidic platform for highly sensitive immunoassays in point-of-care settings. The platform consists of a disposable cartridge containing structures for assay processing, a porous membrane and all dried reagents required for the analysis. Additionally, a blister containing a washing buffer is connected to a new aliquoting structure enabling the serial aliquoting of washing buffer for repetitive bound-free separation steps. The proof-of-concept for two immunoassays is shown in the cartridge with each requiring only 30 μL of whole blood or plasma as the sample material...
October 18, 2017: Analyst
https://www.readbyqxmd.com/read/29042471/measuring-patient-perception-of-quality-in-a-radiology-department-by-adapting-a-standardized-industry-proven-tool
#9
Farouk Dako, Rick Wray, Omer Awan, Rathan M Subramaniam
Introduction: Our study quantitatively measures patient perception of quality and satisfaction in a PET-CT center at the point of care using a psychometrically validated questionnaire, SERVPERF, to guide subsequent quality improvement interventions. SERVPERF is a survey instrument that captures service quality by measuring performance of various services. It has demonstrated reliability and validity across various industries including healthcare, banking, transportation, telecommunication and higher education...
October 17, 2017: Journal of Nuclear Medicine Technology
https://www.readbyqxmd.com/read/29042390/effect-of-point-of-care-susceptibility-testing-in-general-practice-on-appropriate-prescription-of-antibiotics-for-patients-with-uncomplicated-urinary-tract-infection-a-diagnostic-randomised-controlled-trial
#10
Anne Holm, Gloria Cordoba, Tina Møller Sørensen, Lisbeth Rem Jessen, Niels Frimodt-Møller, Volkert Siersma, Lars Bjerrum
OBJECTIVES: To investigate the effect of adding point-of-care (POC) susceptibility testing to POC culture on appropriate use of antibiotics as well as clinical and microbiological cure for patients with suspected uncomplicated urinary tract infection (UTI) in general practice. DESIGN: Open, individually randomised controlled trial. SETTING: General practice. PARTICIPANTS: Women with suspected uncomplicated UTI, including elderly patients above 65, patients with recurrent UTI and patients with diabetes...
October 16, 2017: BMJ Open
https://www.readbyqxmd.com/read/29042095/can-emergency-physicians-accurately-distinguish-retinal-detachment-from-posterior-vitreous-detachment-with-point-of-care-ocular-ultrasound
#11
Nicola Baker, Richard Amini, Elaine H Situ-LaCasse, Josie Acuña, Tomas Nuño, Uwe Stolz, Srikar Adhikari
STUDY OBJECTIVE: There is significant overlap between the symptoms of patients presenting with retinal detachment (RD) and posterior vitreous detachment (PVD). Urgency to obtain consultation and treatment are dependent on the ability to accurately distinguish these two conditions. The objective of this study was to determine the ability of emergency physicians to differentiate RDs from PVDs using point-of-care (POC) ocular ultrasound. METHODS: Single blinded cross-sectional study at an academic medical center...
October 13, 2017: American Journal of Emergency Medicine
https://www.readbyqxmd.com/read/29040777/efficient-imaging-examining-the-value-of-ultrasound-in-the-diagnosis-of-traumatic-adult-brachial-plexus-injuries-a-systematic-review
#12
Brian Chin, Maleka Ramji, Forough Farrokhyar, James R Bain
INTRODUCTION: Traumatic brachial plexus injury (BPI) can result in debilitating sequelae of the upper extremity. Presently, therapeutic decisions are based on the mechanism of injury, serial physical examination, electromyography, nerve conduction, and imaging studies. While magnetic resonance imaging is the current imaging modality of choice for BPI, ultrasound is a promising newcomer that is inexpensive, accessible, and available at point of care. OBJECTIVE: To evaluate ultrasound as a diagnostic tool in the assessment of traumatic adult BPI through a systematic review...
October 11, 2017: Neurosurgery
https://www.readbyqxmd.com/read/29040621/current-status-of-point-of-care-testing-for-human-immunodeficiency-virus-drug-resistance
#13
Horacio A Duarte, Nuttada Panpradist, Ingrid A Beck, Barry Lutz, James Lai, Ruth M Kanthula, Rami Kantor, Anubhav Tripathi, Shanmugam Saravanan, Iain J MacLeod, Michael H Chung, Guoqing Zhang, Chunfu Yang, Lisa M Frenkel
Healthcare delivery has advanced due to the implementation of point-of-care testing, which is often performed within minutes to hours in minimally equipped laboratories or at home. Technologic advances are leading to point-of-care kits that incorporate nucleic acid-based assays, including polymerase chain reaction, isothermal amplification, ligation, and hybridization reactions. As a limited number of single-nucleotide polymorphisms are associated with clinically significant human immunodeficiency virus (HIV) drug resistance, assays to detect these mutations have been developed...
October 13, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29039263/integrating-family-planning-services-into-hiv-care-use-of-a-point-of-care-electronic-medical-record-system-in-lilongwe-malawi
#14
Hannock Tweya, Caryl Feldacker, Lisa B Haddad, Chimango Munthali, Mwatha Bwanali, Colin Speight, Layout G Kachere, Petros Tembo, Sam Phiri
BACKGROUND: Integrating family planning (FP) services into human immunodeficiency virus (HIV) clinical care helps improve access to contraceptives for women living with HIV. However, high patient volumes may limit providers' ability to counsel women about pregnancy risks and contraceptive options. OBJECTIVES: To assess trends in the use of contraceptive methods after implementing an  electronic medical record (EMR) system with FP questions and determine the reasons for non-use of contraceptives among women of reproductive age (15-49 years) receiving antiretroviral therapy (ART) at the Martin Preuss Center clinic in Malawi...
2017: Global Health Action
https://www.readbyqxmd.com/read/29037030/semisynthetic-bioluminescent-sensor-proteins-for-direct-detection-of-antibodies-and-small-molecules-in-solution
#15
Remco Arts, Susann Katrina Julie Ludwig, Benice C B van Gerven, Eva Magdalena Estirado, Lech-Gustav Milroy, Maarten Merkx
Single-step immunoassays that can be performed directly in solution are ideally suited for point-of-care diagnostics. Our group recently developed a new platform of bioluminescent sensor proteins (LUMABS; LUMinescent AntiBody Sensor) that allow antibody detection in blood plasma. Thus far, LUMABS has been limited to the detection of antibodies recognizing natural peptide epitopes. Here, we report the development of semisynthetic LUMABS sensors that recognize non-peptide epitopes. The non-natural amino acid para-azidophenylalanine was introduced at the position of the original antibody-recognition sites as a chemical handle to enable site-specific conjugation of synthetic epitope molecules coupled to a dibenzocylcooctyne moiety via strain-promoted click chemistry...
October 17, 2017: ACS Sensors
https://www.readbyqxmd.com/read/29036895/low-cost-lab-on-chip-for-the-colorimetric-detection-of-nitrate-in-mineral-water-products
#16
Mohammad F Khanfar, Wisam Al-Faqheri, Ala'aldeen Al-Halhouli
The diagnostics of health status and the quality of drinking water are among the most important United Nations sustainable development goals. However, in certain areas, wars and instability have left millions of people setting in refugee camps and dangerous regions where infrastructures are lacking and rapid diagnostics of water quality and medical status are critical. In this work, microfluidic testing chips and photometric setups are developed in cheap and portable way to detect nitrate concentrations in water...
October 14, 2017: Sensors
https://www.readbyqxmd.com/read/29036004/the-utility-of-clinical-ultrasonography-in-identifying-distal-forearm-fractures-in-the-pediatric-emergency-department
#17
Iratxe Galletebeitia Laka, Frederic Samson, Inigo Gorostiza, Andrés Gonzalez, Carlos Gonzalez
OBJECTIVE: The aim of this study is to evaluate the utility of Point-Of-Care Ultrasound (POCUS) for diagnosing distal forearm fractures in pediatric emergency departments (ED). PATIENTS AND METHODS: A prospective observational study was carried out in children aged younger than 15 years attended in an ED with an indication of radiography because of a suspected distal forearm fracture. The POCUS was performed considering cortical irregularity or disruption compatible with fracture...
October 13, 2017: European Journal of Emergency Medicine: Official Journal of the European Society for Emergency Medicine
https://www.readbyqxmd.com/read/29034791/point-of-care-testing-in-the-pharmacy-how-is-the-field-evolving
#18
Donald G Klepser, Michael E Klepser
No abstract text is available yet for this article.
October 16, 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/29034781/point-of-care-testing-in-community-pharmacies-keys-to-success-from-pennsylvania-pharmacists
#19
Emily A Steltenpohl, Brandon K Barry, Kim C Coley, Melissa S McGivney, Julie L Olenak, Lucas A Berenbrok
BACKGROUND: Clinical Laboratory Improvement Amendments (CLIA)-waived tests allow for quick, accurate, and noninvasive laboratory testing. Community pharmacists utilize CLIA-waived tests to provide clinical services such as point-of-care (POC) testing to help manage chronic disease and acute illness. OBJECTIVE: To identify key themes in the successful delivery of POC testing services by community pharmacists in Pennsylvania. RESULTS: An initial search identified 51 Pennsylvania pharmacies with a CLIA waiver...
January 1, 2017: Journal of Pharmacy Practice
https://www.readbyqxmd.com/read/29034303/validation-of-capillary-blood-analysis-and-capillary-testing-mode-on-the-epoc-point-of-care-system
#20
Jing Cao, Rachel Edwards, Janette Chairez, Sridevi Devaraj
BACKGROUND: Laboratory test in transport is a critical component of patient care, and capillary blood is a preferred sample type particularly in children. This study evaluated the performance of capillary blood testing on the epoc Point of Care Blood Analysis System (Alere Inc). METHODS: Ten fresh venous blood samples was tested on the epoc system under the capillary mode. Correlation with GEM 4000 (Instrumentation Laboratory) was examined for Na+, K+, Cl-, Ca2+, glucose, lactate, hematocrit, hemoglobin, pO2, pCO2, and pH, and correlation with serum tested on Vitros 5600 (Ortho Clinical Diagnostics) was examined for creatinine...
December 2017: Pract Lab Med
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