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https://www.readbyqxmd.com/read/29684911/high-throughput-simultaneous-quantitation-of-hemoglobin-adducts-of-acrylamide-glycidamide-and-ethylene-oxide-using-uhplc-ms-ms
#1
Min Yang, Tunde Frame, Chui Tse, Hubert W Vesper
Ethylene oxide (EO), acrylamide (AA) and glycidamide (GA) exposures are associated with mammary tumors in animals. Currently available information about human exposure to these chemicals is limited creating the need for analytical methods to assess their exposure. We developed a sensitive ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method to simultaneously quantitate hemoglobin (Hb) N-terminal valine adducts of AA (HbAA), GA (HbGA), and EO (HbEO) using modified Edman reaction...
April 3, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29684902/effect-of-lipopeptide-structure-on-gene-delivery-system-properties-evaluation-in-2d-and-3d-in-vitro-models
#2
O O Koloskova, A M Gileva, M G Drozdova, M V Grechihina, N E Suzina, U A Budanova, Yu L Sebyakin, D A Kudlay, I P Shilovskiy, A M Sapozhnikov, E I Kovalenko, E A Markvicheva, M R Khaitov
Development of efficient biodegradable, environmentally responsive, biocompatible and non-toxic delivery system is needed for efficient gene delivery. As well known, properties of the vehicle are determined by the structure of carrier components. The aim of the current study was to estimate in vitro transfection efficacy of aliphatic di-, tri- and tetrapeptide-based cationic lipoplexes loaded with siRNA in function of a number of cationic groups using 2D (monolayer culture) and 3D (multicellular tumor spheroids) in vitro models...
April 7, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29684863/psychological-and-genetic-risk-factors-associated-with-suicidal-behavior-in-korean-patients-with-mood-disorders
#3
Hee-Yeon Choi, Ga Eun Kim, Kyoung Ae Kong, Yu Jin Lee, Weon-Jeong Lim, So-Hyun Park, Su Hee Ha, Soo In Kim
INTRODUCTION: To prevent suicide, a nationwide important issue in Korea, understanding its etiology is essential. This study aimed to delineate the risk factors for suicidal behavior in mood disorder patients in Korea. METHODS: 216 patients with mood disorders were recruited. We measured suicidal ideation (SI) and suicide attempt (SA) history and the association of six gene polymorphisms with SB: COMT (rs4680), HTR1A (rs6295), TPH1 (rs1800532), BDNF (rs6265), SLC1A3 (rs2269272), and SLC1A2 (rs4755404)...
April 10, 2018: Journal of Affective Disorders
https://www.readbyqxmd.com/read/29684785/photoreceptor-calcium-sensor-proteins-in-detergent-resistant-membrane-rafts-are-regulated-via-binding-to-caveolin-1
#4
Vasiliy I Vladimirov, Evgeni Yu Zernii, Viktoriia E Baksheeva, Hanna Wimberg, Alexey S Kazakov, Natalya K Tikhomirova, Ekaterina L Nemashkalova, Vladimir A Mitkevich, Andrey A Zamyatnin, Valery M Lipkin, Pavel P Philippov, Sergei E Permyakov, Ivan I Senin, Karl-W Koch, Dmitry V Zinchenko
Rod cell membranes contain cholesterol-rich detergent-resistant membrane (DRM) rafts, which accumulate visual cascade proteins as well as proteins involved in regulation of phototransduction such as rhodopsin kinase and guanylate cyclases. Caveolin-1 is the major integral component of DRMs, possessing scaffolding and regulatory activities towards various signaling proteins. In this study, photoreceptor Ca2+ -binding proteins recoverin, NCS1, GCAP1, and GCAP2, belonging to neuronal calcium sensor (NCS) family, were recognized as novel caveolin-1 interacting partners...
April 14, 2018: Cell Calcium
https://www.readbyqxmd.com/read/29684709/the-role-of-natural-selection-in-shaping-genetic-variation-in-a-promising-chagas-disease-drug-target-trypanosoma-cruzi-trans-sialidase
#5
Joseph P Gallant, Raquel Asunción Lima-Cordón, Silvia Justi, Maria Carlota Monroy, Toni Viola, Lori Stevens
Rational drug design creates innovative therapeutics based on knowledge of the biological target to provide more effective and responsible therapeutics. Chagas disease, endemic throughout Latin America, is caused by Trypanosoma cruzi, a protozoan parasite. Current therapeutics are problematic with widespread calls for new approaches. Researchers are using rational drug design for Chagas disease and one target receiving considerable attention is the T. cruzi trans-sialidase protein (TcTS). In T. cruzi, trans-sialidase catalyzes the transfer of sialic acid from a mammalian host to coat the parasite surface membrane and avoid immuno-detection...
April 20, 2018: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/29684673/exercise-as-a-therapeutic-intervention-to-optimize-fetal-weight
#6
REVIEW
Laura M Reyes, Margie H Davenport
The Developmental Origins of Health and Disease suggest the in utero environment programs offspring obesity and cardiovascular disease. Therefore, there is a need to implement safe therapeutic interventions that do not involve the intake of medications or biological products during pregnancy that can improve maternal and fetal health. Prenatal exercise is established to promote maternal and fetal health. It is generally recommended that women accumulate at least 150 minutes per week of moderate-intensity exercise...
April 20, 2018: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/29684624/component-effect-of-stem-cell-loaded-thermosensitive-polypeptide-hydrogels-on-cartilage-repair
#7
He Liu, Yilong Cheng, Jinjin Chen, Fei Chang, Jincheng Wang, Jianxun Ding, Xuesi Chen
Biophysical properties of the desired biomimetic scaffolds, such as porosity and elasticity, have been proven associated with the efficacy of cartilage regeneration. In this work, the copolymers of poly(L-alanine)-block-poly(ethylene glycol)-block-poly(L-alanine) (PA-PEG-PA) and poly(L-alanine-co-L-phenylalanine)-block-poly(ethylene glycol)-block-poly(L-alanine-co-L-phenylalanine) (PAF-PEG-PAF) with different ratios of alanine to phenylalanine were synthesized. The introduction of a hydrophobic amino acid, phenylalanine, into polyalanine-based thermosensitive hydrogel leaded to the enhanced gelation behaviors and upregulated mechanical properties...
April 20, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29684594/new-apetx-like-peptides-from-sea-anemone-heteractis-crispa-modulate-asic1a-channels
#8
Rimma Kalina, Irina Gladkikh, Pavel Dmitrenok, Oleg Chernikov, Sergey Koshelev, Aleksandra Kvetkina, Sergey Kozlov, Emma Kozlovskaya, Margarita Monastyrnaya
Sea anemones are an abundant source of various biologically active peptides. The hydrophobic 20% ethanol fraction of tropical sea anemone Heteractis crispa was shown to contain at least 159 peptide compounds including neurotoxins, proteinase and α-amylase inhibitors, as well as modulators of acid-sensing ion channels (ASICs). The three new peptides, π-AnmTX Hcr 1b-2, -3, and -4 (41 aa) (short names Hcr 1b-2, -3, -4), identified by a combination of reversed-phase liquid chromatography and mass spectrometry were found to belong to the class 1b sea anemone neurotoxins...
April 20, 2018: Peptides
https://www.readbyqxmd.com/read/29684557/regiospecificity-of-a-novel-bacterial-lipoxygenase-from-myxococcus-xanthus-for-polyunsaturated-fatty-acids
#9
Jung-Ung An, Seung-Hye Hong, Deok-Kun Oh
Lipoxygenase (LOX) is the key enzyme involved in the synthesis of oxylipins as signaling compounds that are important for cell growth and development, inflammation, and pathogenesis in various organisms. The regiospecificity of LOX from Myxococcus xanthus, a gram-negative bacterium, was investigated. The enzyme catalyzed oxygenation at the n-9 position in C20 and C22 polyunsaturated fatty acids (PUFAs) to form 12S- and 14S-hydroxy fatty acids (HFAs), respectively, and oxygenation at the n-6 position in C18 PUFAs to form 13-HFAs...
April 20, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29684490/phosphorylated-nucleolar-tau-protein-is-related-to-the-neuronal-in-vitro-differentiation
#10
Concetta Federico, Laura Gil, Francesca Bruno, Agata Grazia D'Amico, Velia D'Agata, Salvatore Saccone
Tau is a multifunctional protein, originally identified as a cytoplasmic protein associated with microtubules. It is codified by the MAPT gene, and the alternative splicing, in the neuronal cells, results in six different isoforms. Tau was subsequently observed in the cell nucleus, where its function is not yet clearly understood. Here, we studied the MAPT gene and the cellular localization of the AT8 and Tau-1 epitopes of Tau protein, in the SK-N-BE cell line, which differentiates in neuronal-like cells after retinoic acid treatment...
April 20, 2018: Gene
https://www.readbyqxmd.com/read/29684455/a-novel-extracellular-copper-zinc-superoxide-dismutase-identified-from-nibea-albiflora-and-its-characteristics-under-ammonia-nitrite-stress
#11
Xiaolong Wang, Qing Song, Zhiyong Wang, Fang Han
The cDNA of EcSOD (designated as NaEcSOD), cloned and characterized from Nibea albiflora for the first time, was 1101 bp in length including 5'-untranslated region (UTR) of 224 bp, 3' UTR of 229 bp, and an open reading frame (ORF) of 648 bp encoding a polypeptide of 215 amino acids. Based on the results of GST pull-down assay and mass spectrometric identifications, a membrane protein (annexin A4) was found to specifically bind with NaEcSOD, indicating that the two proteins might be involved in antagonizing reactive oxygen species (ROS) together...
April 20, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29684441/cloning-and-characterization-of-apcystatin-a-plant-cystatin-gene-from-agapanthus-praecox-ssp-orientalis-responds-to-abiotic-stress
#12
Guan-Qun Chen, Di Zhang, Xiao-Hui Shen
Plant cystatins are involved in the regulation of protein turnover and play important roles in defense mechanisms. We cloned the ApCystatin gene from Agapanthus praecox ssp. orientalis, a famous ornamental and medical plant. The complete cDNA sequence of ApCystatin is comprised of 1446 nucleotides with a 420 bp ORF encoding 140 amino acids. The mRNA level of ApCystatin was significantly up-regulated under various abiotic stress, such as salt, osmosis, oxidative and cold stresses, which suggested that ApCystatin participated in the plant's resistance to stress...
April 20, 2018: Protein Expression and Purification
https://www.readbyqxmd.com/read/29684432/intraspecific-mitochondrial-genome-comparison-identified-cytb-as-a-high-resolution-population-marker-in-a-new-pest-athetis-lepigone
#13
Lei Chen, Jian-Rong Huang, Jun Dai, Yan-Fei Guo, Jing-Tao Sun, Xiao-Yue Hong
A new outbreak pest, Athetis lepigone (Möschler) (Lepidoptera: Noctuidae), has caused severe economic loss in maize crops in China. In order to conduct population genetics study with a more polymorphic and scientific mitochondrial marker, we sequenced the complete mitochondrial genomes of 13 different A. lepigone individuals. Intraspecific comparison of all PCGs showed that the NADH dehydrogenase and cytochrome b genes had the highest nucleotide diversity. We also found evidence of episodic positive selection on two amino acids, which are encoded by NADH dehydrogenase genes (ND3 and ND4L), against a background of widespread neutral selection of all other mitochondrial PCGs...
April 20, 2018: Genomics
https://www.readbyqxmd.com/read/29684348/crystal-structure-of-a-thermophilic-o-6-alkylguanine-dna-alkyltransferase-derived-self-labeling-protein-tag-in-covalent-complex-with-a-fluorescent-probe
#14
Franca Rossi, Castrese Morrone, Alberto Massarotti, Davide Maria Ferraris, Anna Valenti, Giuseppe Perugino, Riccardo Miggiano
The self-labeling protein tags are robust and versatile tools for studying different molecular aspects of cell biology. In order to be suitable for a wide spectrum of experimental conditions, it is mandatory that these systems are stable after the fluorescent labeling reaction and do not alter the properties of the fusion partner. SsOGT-H5 is an engineered variant alkylguanine-DNA-alkyl-transferase (OGT) of the hyperthermophilic archaeon Sulfolobus solfataricus, and it represents an alternative solution to the SNAP-tag® technology under harsh reaction conditions...
April 20, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29684324/taxonomic-distribution-structure-function-relationship-and-metabolic-context-of-the-two-families-of-sulfide-dehydrogenases-sqr-and-fcsd
#15
Filipe M Sousa, Juliana G Pereira, Bruno C Marreiros, Manuela M Pereira
Hydrogen sulfide (H2 S) is a versatile molecule with different functions in living organisms: it can work as a metabolite of sulfur and energetic metabolism or as a signaling molecule in higher Eukaryotes. H2 S is also highly toxic since it is able to inhibit heme cooper oxygen reductases, preventing oxidative phosphorylation. Due to the fact that it can both inhibit and feed the respiratory chain, the immediate role of H2 S on energy metabolism crucially relies on its bioavailability, meaning that studying the central players involved in the H2 S homeostasis is key for understanding sulfide metabolism...
April 20, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29684281/superactivity-of-mof-808-towards-peptide-bond-hydrolysis
#16
Hong Giang T Ly, Guangxia Fu, Aleksandar Kondinski, Bart Bueken, Dirk De Vos, Tatjana N Parac-Vogt
MOF-808, a Zr(IV)-based metal-organic framework, has been proven to be a very effective heterogeneous catalyst for the hydrolysis of the peptide bond in a wide range of peptides and in hen egg white lysozyme protein. The kinetic experiments with a series of Gly-X dipeptides with varying nature of amino acid side chain have shown that MOF-808 exhibits selectivity depending on the size and chemical nature of the X side chain. Dipeptides with smaller or hydrophilic residues were hydrolyzed faster than those with bulky and hydrophobic residues that lack electron rich functionalities which could engage in favourable intermolecular interactions with the btc linkers...
April 23, 2018: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29684073/nutrition-versus-defense-why-myzus-persicae-green-peach-aphid-prefers-and-performs-better-on-young-leaves-of-cabbage
#17
He-He Cao, Zhan-Feng Zhang, Xiao-Feng Wang, Tong-Xian Liu
Plant leaves of different ages differ in nutrients and toxic metabolites and thus exhibit various resistance levels against insect herbivores. However, little is known about the influence of leaf ontogeny on plant resistance to phloem-feeding insects. In this study, we found that the green peach aphid, Myzus persicae, preferred to settle on young cabbage leaves compared with mature or old leaves, although young leaves contained the highest concentration of glucosinolates. Furthermore, aphids feeding on young leaves had higher levels of glucosinolates in their body, but aphids performed better on young leaves in terms of body weight and population growth...
2018: PloS One
https://www.readbyqxmd.com/read/29684072/arginine-is-a-component-of-the-ammonium-cyg56-signalling-cascade-that-represses-genes-of-the-nitrogen-assimilation-pathway-in-chlamydomonas-reinhardtii
#18
David González-Ballester, Emanuel Sanz-Luque, Aurora Galván, Emilio Fernández, Amaury de Montaigu
Nitrogen assimilation and metabolism are essential processes for all living organisms, yet there is still much to be learnt on how they are regulated. The use of Chlamydomonas reinhardtii as a model system has been instrumental not only in identifying conserved regulation mechanisms that control the nitrogen assimilation pathway, but also in understanding how the intracellular nitrogen status regulates metabolic processes of industrial interest such as the synthesis of biolipids. While the genetic regulators that control the nitrogen pathway are successfully being unravelled, other layers of regulation have received less attention...
2018: PloS One
https://www.readbyqxmd.com/read/29684065/biochemical-properties-of-l-arabinose-isomerase-from-clostridium-hylemonae-to-produce-d-tagatose-as-a-functional-sweetener
#19
Tien-Kieu Nguyen, Moon-Gi Hong, Pahn-Shick Chang, Byung-Hoo Lee, Sang-Ho Yoo
d-Tagatose has gained substantial interest due to its potential functionalities as a sucrose substitute. In this study, the gene araA, encoding l-arabinose isomerase (l-AI) from Clostridium hylemonae (DSM 15053), was cloned and expressed in Escherichia coli BL21 (DE3). This gene consists of 1,506 nucleotides and encodes a protein of 501 amino acid residues with a calculated molecular mass of 56,554 Da. Since l-AI was expressed as an intracellular inclusion body, this enzyme was solubilized with guanidine hydrochloride, refolded, and activated with a descending concentration gradient of urea...
2018: PloS One
https://www.readbyqxmd.com/read/29684050/intronic-pah-gene-mutations-cause-a-splicing-defect-by-a-novel-mechanism-involving-u1snrnp-binding-downstream-of-the-5-splice-site
#20
Ainhoa Martínez-Pizarro, Maja Dembic, Belén Pérez, Brage S Andresen, Lourdes R Desviat
Phenylketonuria (PKU), one of the most common inherited diseases of amino acid metabolism, is caused by mutations in the phenylalanine hydroxylase (PAH) gene. Recently, PAH exon 11 was identified as a vulnerable exon due to a weak 3' splice site, with different exonic mutations affecting exon 11 splicing through disruption of exonic splicing regulatory elements. In this study, we report a novel intron 11 regulatory element, which is involved in exon 11 splicing, as revealed by the investigated pathogenic effect of variants c...
April 23, 2018: PLoS Genetics
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