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https://www.readbyqxmd.com/read/29458126/structural-and-mechanistic-analysis-of-the-choline-sulfatase-from-sinorhizobium-melliloti-a-class-i-sulfatase-specific-for-an-alkyl-sulfate-ester
#1
Bert van Loo, Markus Schober, Eugene Valkov, Magdalena Heberlein, Erich Bornberg-Bauer, Kurt Faber, Marko Hyvönen, Florian Hollfelder
Hydrolysis of organic sulfate esters proceeds by two distinct mechanisms, water attacking at either sulfur (S-O bond cleavage) or carbon (C-O bond cleavage). In primary and secondary alkyl sulfates attack at carbon is favored, whereas in aromatic sulfates and sulfated sugars attack at sulfur is preferred. This mechanistic distinction is mirrored in the classification of enzymes that catalyze sulfate ester hydrolysis: arylsulfatases catalyze S-O cleavage in sulfate sugars and arylsulfates and alkyl sulfatases break the C-O bond of alkyl sulfates...
February 16, 2018: Journal of Molecular Biology
https://www.readbyqxmd.com/read/29458094/a-conserved-interferon-regulation-factor-1-irf-1-from-pacific-oyster-crassostrea-gigas-functioned-as-an-activator-of-ifn-pathway
#2
Mengmeng Lu, Chuanyan Yang, Meijia Li, Qilin Yi, Guangxia Lu, Yichen Wu, Chen Qu, Lingling Wang, Linsheng Song
Interferon regulatory factors (IRFs), a family of transcription factors with a novel helix-turn-helix DNA-binding motif, play important roles in regulating the expression of interferons (IFNs) and IFN-stimulated genes. In the present study, an interferon regulation factor 1 was identified from oyster Crassostrea gigas (designated CgIRF-1), and its immune function was characterized to understand the regulatory mechanism of interferon system against viral infection in invertebrates. The open reading frame (ORF) of CgIRF-1 was 993 bp, encoding a polypeptide of 330 amino acids with a typical IRF domain (also known as DNA-binding domain)...
February 16, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29458023/structural-characterization-of-the-hcov-229e-fusion-core
#3
Wei Zhang, Qianqian Zheng, Mengrong Yan, Xiaobo Chen, Haitao Yang, Weihong Zhou, Zihe Rao
HCoV-229E spike (S) protein mediates virion attachment to cells and subsequent fusion of the viral and cellular membranes. This protein is composed of an N-terminal receptor-binding domain (S1) and a C-terminal trans-membrane fusion domain (S2). S2 contains a highly conserved heptad repeat 1 and 2 (HR1 and HR2). In this study, the HRs sequences were designed and connected with a flexible linker. The recombinant fusion core protein was crystallized and its structure was solved at a resolution of 2.45 Å. Then we characterized the binding of HR1s and HR2s via both sequence alignment and structural analysis...
February 16, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29458014/topical-delivery-of-l-theanine-ameliorates-tpa-induced-acute-skin-inflammation-via-downregulating-endothelial-pecam-1-and-neutrophil-infiltration-and-activation
#4
Wei-Jie Zeng, Zhi Tan, Xing-Fei Lai, Ya-Nan Xu, Chun-Lin Mai, Jun Zhang, Zhen-Jia Lin, Xian-Guo Liu, Shi-Li Sun, Li-Jun Zhou
l-theanine, the most abundant free amino acid in tea, has been documented to possess many different bioactive properties through oral or intragastrical delivery. However, little is known about the effect of topical delivery of l-theanine on acute inflammation. In the present study, by using 12-O-tetradecanoylphorbol-13-acetate (TPA, 2.5 μg/ear)-induced ear edema model in mice, we first found that single-dose local pretreatment of l-theanine 30 min before TPA time- and dose-dependently suppressed the increases in both skin thickness and weight...
February 16, 2018: Chemico-biological Interactions
https://www.readbyqxmd.com/read/29457850/preparation-aroma-characteristics-and-volatile-compounds-of-flavorings-from-enzymatic-hydrolyzed-rice-bran-protein-concentrate
#5
Supeeraya Arsa, Chockchai Theerakulkait
BACKGROUND: Rice bran is by-product obtained from the rice milling industry. The aims of this research were to add value of rice bran by preparation of enzymatic hydrolyzed rice bran protein concentrate (HRPC) as a flavoring agent and the flavoring which was produced by HRPC has not been discovered. Different drying methods (freeze drying and spray drying) and fructose additions were studied for improvement of rice bran protein sensorial aroma characteristics. RESULTS: the most abundant amino acids in liquid HRPC (LH) were glutamic acid, arginine, aspartic acid and leucine, respectively...
February 19, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29457824/understanding-the-conformational-analysis-of-gababutin-based-hybrid-peptides
#6
Maruthi Konda, Rohit G Jadhav, Sayan Maiti, Shaikh M Mobin, Brice Kauffmann, Apurba K Das
Constrained γ-amino acid gababutin (Gbn) based peptides that form different conformations have been synthesized. Striving to rationalize the impact of side chain orientations framing tetrapeptide-based supramolecular organic frameworks and morphological entities, Gbn incorporated hybrid peptides Boc-Gbn-Aib-Aaa-Aib-OMe (where Aaa = Phe(F) for peptide 1, Leu(L) for peptide 2 and Tyr(Y) for peptide 3) were synthesized by changing the amino acid at the third position. The solution state dual folded conformation (C12 /C10 H-bonded) is probed by 2D NMR spectroscopy in support of a DMSO-d6 titration and VT NMR experiments...
February 19, 2018: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/29457815/hydrogen-bonding-effect-between-active-site-and-protein-environment-on-catalysis-performance-in-h-2-producing-nife-hydrogenases
#7
Siyao Qiu, Luis Miguel Azofra, Douglas R MacFarlane, Chenghua Sun
The interaction between the active site and the surrounding protein environment plays a fundamental role in the hydrogen evolution reaction (HER) in [NiFe] hydrogenases. Our density functional theory (DFT) findings demonstrate that the reaction Gibbs free energy required for the rate determining step decreases by 7.1 kcal mol-1 when the surrounding protein environment is taken into account, which is chiefly due to free energy decreases for the two H+ /e- addition steps (the so-called Ni-SIa to I1, and Ni-C to Ni-R), being the largest thermodynamic impediments of the whole reaction...
February 19, 2018: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/29457714/selective-recognition-of-rna-substrates-by-adar-deaminase-domains
#8
Yuru Wang, SeHee Park, Peter A Beal
Adenosine deamination is one of the most prevalent post-transcriptional modifications in mRNA and is catalyzed by ADAR1 and ADAR2 in humans. ADAR1 and ADAR2 have different substrate selectivity, which is believed to mainly originate from the proteins' deaminase domains (hADAR1d and hADAR2d, respectively). RNA-seq of the S. cerevisiae transcriptome subjected to ADAR-catalyzed RNA editing identified substrates with common secondary structure features preferentially edited by hADAR1d over hADAR2d. The relatively small size and efficient reaction of one of these substrates suggested it could be useful for further study of the hADAR1d reaction...
February 19, 2018: Biochemistry
https://www.readbyqxmd.com/read/29457681/analysis-of-rpa190-revealed-multiple-positively-selected-mutations-associated-with-metalaxyl-resistance-in-phytophthora-infestans
#9
Fengping Chen, Qian Zhou, Jing Xi, Dong-Liang Li, Guido Schnabel, Jiasui Zhan
BACKGROUND: Metalaxyl is a phenylamide fungicide, introduced for oomycete management in the 1970s. RPA190 gene has been reported to be associated with metalaxyl resistance, although other genes may be involved in the process as well. The present study assessed the evolutionary association of amino acid (AA) mutations in RPA190 with metalaxyl resistance in Phytophthora infestans. RESULTS: All isolates from Yunnan were resistant to metalaxyl with multiple AA mutations in RPA190...
February 19, 2018: Pest Management Science
https://www.readbyqxmd.com/read/29457601/influences-of-combined-traffic-noise-on-the-ability-of-learning-and-memory-in-mice
#10
Guo-Qing Di, Zhao-Qi Qin
Objective: The present study aimed to evaluate the influences of combined traffic noise (CTN) on the ability of learning and memory in mice. Materials and Methods: The Institute of Cancer Research (ICR) mice were exposed to CTN from highways and high-speed railways for 42 days, whose day-night equivalent continuous A-weighted sound pressure level (Ldn ) was 70 dB(A). On the basis of behavioral reactions in Morris water maze (MWM) and the concentrations of amino acid neurotransmitters in the hippocampus, the impacts of CTN on learning and memory in mice were examined...
January 2018: Noise & Health
https://www.readbyqxmd.com/read/29457581/association-between-tlr2-2477g-a-polymorphism-and-bacterial-meningitis-a-meta-analysis
#11
Xiaochun Jin, Shuzhou Yin, Youtao Zhang, Xu Chen
Toll-like receptor 2 (TLR2) is a key member of TLRs, which is crucial in the initial inflammatory response against bacteria. TLR2, is also the initial barrier against bacterial infection and plays an important role in recognising a variety of bacterial lipoproteins. Several studies have been performed to investigate the TLR2 + 2477G/A polymorphism and bacterial meningitis susceptibility. Unfortunately, the results of previous studies were controversial. Therefore, we performed a meta-analysis to derive a more precise estimation of the association...
February 19, 2018: Epidemiology and Infection
https://www.readbyqxmd.com/read/29457542/exometabolomic-profiling-of-bacterial-strains-cultivated-using-arabidopsis-root-extract-as-the-sole-carbon-source
#12
Richard Paul Jacoby, Anna Martyn, Stan Kopriva
The ability of microorganisms to use root-derived metabolites as growth substrates is a key trait for success in the rhizospheric niche. However, few studies describe which specific metabolites are consumed, or to what degree microbial strains differ in their substrate consumption patterns. Here we present an LC-MS exometabolomic study of three bacterial strains cultivated using either glucose or Arabidopsis thaliana root extract as the sole carbon source. Two of the strains were previously isolated from field-grown Arabidopsis roots, the other is Escherichia coli included as a comparison...
February 19, 2018: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/29457368/emergence-of-two-novel-sublineages-ind2001bd1-and-ind2001bd2-of-foot-and-mouth-disease-virus-serotype-o-in-bangladesh
#13
M A Siddique, M R Ali, A S M R U Alam, H Ullah, A Rahman, R P Chakrabarty, M A Amin, S A Hoque, S P Nandi, M Sultana, M A Hossain
Foot-and-mouth disease (FMD) is endemic in Bangladesh, and the implementation of a control programme for this disease is at an early stage, according to the FAO- and OIE-proposed Progressive Control Pathway for FMD (PCP-FMD) Roadmap. To develop an effective control programme, understanding of foot-and-mouth disease virus (FMDV) serotypes, even subtypes within the serotypes is essential. The present investigation aims at viral VP1 coding region sequence-based analysis of FMD samples collected from 34 FMD outbreaks during 2012-2016 in Bangladesh...
February 18, 2018: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29456970/the-complement-binding-and-inhibitory-protein-cbia-of-borrelia-miyamotoi-degrades-extracellular-matrix-components-by-interacting-with-plasmin-ogen
#14
Ngoc T T Nguyen, Florian Röttgerding, Gayatri Devraj, Yi-Pin Lin, Arno Koenigs, Peter Kraiczy
The emerging relapsing fever spirochete Borrelia ( B .) miyamotoi is transmitted by ixodid ticks and causes the so-called hard tick-borne relapsing fever or B. miyamotoi disease (BMD). More recently, we identified a surface-exposed molecule, CbiA exhibiting complement binding and inhibitory capacity and rendering spirochetes resistant to complement-mediated lysis. To gain deeper insight into the molecular principles of B. miyamotoi -host interaction, we examined CbiA as a plasmin(ogen) receptor that enables B...
2018: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29456803/potent-inhibitors-of-hepatitis-c-virus-ns3-protease-employment-of-a-difluoromethyl-group-as-a-hydrogen-bond-donor
#15
Barbara Zheng, Stanley V D'Andrea, Li-Qiang Sun, Alan Xiangdong Wang, Yan Chen, Peter Hrnciar, Jacques Friborg, Paul Falk, Dennis Hernandez, Fei Yu, Amy K Sheaffer, Jay O Knipe, Kathy Mosure, Ramkumar Rajamani, Andrew C Good, Kevin Kish, Jeffrey Tredup, Herbert E Klei, Manjula Paruchuri, Alicia Ng, Qi Gao, Richard A Rampulla, Arvind Mathur, Nicholas A Meanwell, Fiona McPhee, Paul M Scola
The design and synthesis of potent, tripeptidic acylsulfonamide inhibitors of HCV NS3 protease that contain a difluoromethyl cyclopropyl amino acid at P1 are described. A cocrystal structure of 18 with a NS3/4A protease complex suggests the presence of a H-bond between the polarized C-H of the CHF2 moiety and the backbone carbonyl of Leu135 of the enzyme. Structure-activity relationship studies indicate that this H-bond enhances enzyme inhibitory potency by 13- and 17-fold compared to the CH3 and CF3 analogues, respectively, providing insight into the deployment of this unique amino acid...
February 8, 2018: ACS Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/29456680/correlation-of-vitamin-d-receptor-with-bronchial-asthma-in-children
#16
Chunlei Hou, Xiaoli Zhu, Xiangyun Chang
This study was designed to investigate the correlation of vitamin D receptor (VDR) gene polymorphism with bronchial asthma in children. Seventy patients admitted to Daqing Longnan Hospital and diagnosed as bronchial asthma for the first time from April 2015 to May 2017 were selected as observation group. Patients received routine treatment and intervention. Seventy healthy subjects admitted to hospital during the same period were enrolled as the control group. Vitamin D gene polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism assay; the levels of total serum immunoglobulin E (IgE) in the two groups were determined by electrochemiluminescence immunoassay; lung function levels in patients were measured using PowerCube (Germany) pulmonary function instrument before and after treatment, and the relationship between VDR gene polymorphism and lung function in children with bronchial asthma was analyzed...
March 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29456627/growth-and-lipid-accumulation-by-different-nutrients-in-the-microalga-chlamydomonas-reinhardtii
#17
Lei Yang, Jun Chen, Shan Qin, Min Zeng, Yongguang Jiang, Lang Hu, Peng Xiao, Wenlong Hao, Zhangli Hu, Anping Lei, Jiangxin Wang
Background: Individual nutrient depletion is widely used to induce lipid accumulation in microalgae, which also causes cell growth inhibition and decreases the total biomass. Thus, improving the lipid accumulation without biomass loss in the nutrient deficiency cells becomes a potential cost-effective treatment for cheaper biofuels. Methods: In this study, the effects of different nutritional conditions on the growth and contents of lipids in Chlamydomonas reinhardtii were compared, and the metabolic profiles under different nutritional conditions were also investigated...
2018: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29456586/dietary-canolol-protects-the-heart-against-the-deleterious-effects-induced-by-the-association-of-rapeseed-oil-vitamin-e-and-coenzyme-q10-in-the-context-of-a-high-fat-diet
#18
Thibault Leger, Isabelle Hininger-Favier, Frédéric Capel, Alain Geloen, Jean-Paul Rigaudière, Chrystèle Jouve, Elodie Pitois, Gaelle Pineau, Carole Vaysse, Jean-Michel Chardigny, Marie-Caroline Michalski, Corinne Malpuech-Brugère, Luc Demaison
Background: Obesity progressively leads to cardiac failure. Omega-3 polyunsaturated fatty acids (PUFA) have been shown to have cardio-protective effects in numerous pathological situations. It is not known whether rapeseed oil, which contains α-linolenic acid (ALA), has a similar protective effect. Omega-3 PUFAs are sensitive to attack by reactive oxygen species (ROS), and lipid peroxidation products could damage cardiac cells. We thus tested whether dietary refined rapeseed oil (RSO) associated with or without different antioxidants (vitamin E, coenzyme Q10 and canolol) is cardio-protective in a situation of abdominal obesity...
2018: Nutrition & Metabolism
https://www.readbyqxmd.com/read/29456532/a-stretch-of-negatively-charged-amino-acids-of-linker-for-activation-of-t-cell-adaptor-has-a-dual-role-in-t-cell-antigen-receptor-intracellular-signaling
#19
Mikel M Arbulo-Echevarria, Isaac Narbona-Sánchez, Cecilia M Fernandez-Ponce, Inmaculada Vico-Barranco, Mª Dolores Rueda-Ygueravide, Michael L Dustin, Arkadiusz Miazek, Mª Carmen Duran-Ruiz, Francisco García-Cózar, Enrique Aguado
The adaptor protein linker for activation of T cells (LAT) has an essential role transducing activatory intracellular signals coming from the TCR/CD3 complex. Previous reports have shown that upon T-cell activation, LAT interacts with the tyrosine kinase Lck, leading to the inhibition of its kinase activity. LAT-Lck interaction seemed to depend on a stretch of negatively charged amino acids in LAT. Here, we have substituted this segment of LAT between amino acids 113 and 126 with a non-charged segment and expressed the mutant LAT (LAT-NIL) in J...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29456505/deletion-of-trace-amine-associated-receptor-1-attenuates-behavioral-responses-to-caffeine
#20
Michael D Schwartz, Jeremiah B Palmerston, Diana L Lee, Marius C Hoener, Thomas S Kilduff
Trace amines (TAs), endogenous amino acid metabolites that are structurally similar to the biogenic amines, are endogenous ligands for trace amine-associated receptor 1 (TAAR1), a GPCR that modulates dopaminergic, serotonergic, and glutamatergic activity. Selective TAAR1 full and partial agonists exhibit similar pro-cognitive, antidepressant- and antipsychotic-like properties in rodents and non-human primates, suggesting TAAR1 as a novel target for the treatment of neurological and psychiatric disorders. We previously reported that TAAR1 partial agonists are wake-promoting in rats and mice, and that TAAR1 knockout (KO) and overexpressing mice exhibit altered sleep-wake and EEG spectral composition...
2018: Frontiers in Pharmacology
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