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https://www.readbyqxmd.com/read/28533794/boron-supply-enhances-aluminum-tolerance-in-root-border-cells-of-pea-pisum-sativum-by-interacting-with-cell-wall-pectins
#1
Xue Wen Li, Jia You Liu, Jing Fang, Lin Tao, Ren Fang Shen, Ya Lin Li, Hong Dong Xiao, Ying Ming Feng, Hai Xiang Wen, Jia Hua Guan, Li Shu Wu, Yong Ming He, Heiner E Goldbach, Min Yu
Aluminum (Al) toxicity is the primary factor limiting crop growth in acidic soils. Boron (B) alleviates Al toxicity in plants, which is mainly considered to be due to the formation of Rhamnogalacturonan II-B (RGII-B) complexes, which helps to stabilize the cytoskeleton. It is unclear yet whether this is due to the increasing of net negative charges and/or further mechanisms. Kinetics of Al accumulation and adsorption were investigated using entire cells, cell wall and pectin of root border cells (RBCs) of pea (Pisum sativum), to reveal the mechanism of B in interacting with alkali-soluble and chelator-soluble pectin for an increased Al tolerance in RBCs...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28530606/effects-of-chemical-composition-and-baking-on-in-vitro-digestibility-of-proteins-in-breads-made-from-selected-gluten-containing-and-gluten-free-flours
#2
Tong Wu, Cheryl Taylor, Thomas Nebl, Ken Ng, Louise E Bennett
Breads prepared from cereal grains are a dietary staple, providing a significant proportion of daily energy, but not necessarily of dietary protein. However, good digestibility of proteins in bread is important to avoid potential immunogenic effects of undigested peptides, including for those gluten-intolerant. Four gluten-containing (white wheat, wholemeal wheat, spelt and rye) and four gluten-free (chick pea, lupin, buckwheat, amaranth) flours were used to make yeast-leavened breads standardized for protein...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28530187/application-of-snps-to-improve-yield-of-pisum-sativum-l-pea
#3
Ansar Mehmood, Ghulam Murtaza
Nanotechnology opens an enormous scope of novel application in the fields of biotechnology and agricultural industries, because nanoparticles (NPs) have unique physicochemical properties, i.e. high surface area, high reactivity, tunable pore size and particle morphology. Present study was carried out to determine the role of silver NPs (SNPs) to improve yield of Pisum sativum L. SNPs (10-100 nm) were synthesised by green method using extract of Berberis lycium Royle. Pea seeds were soaked and seedling were foliage sprayed by 0, 30, 60 and 90 ppm SNPs...
June 2017: IET Nanobiotechnology
https://www.readbyqxmd.com/read/28528258/epidemiology-and-management-of-chronic-thromboembolic-pulmonary-hypertension-experience-from-two-expert-centers
#4
Maria Anna Bazmpani, Alexandra Arvanitaki, Maria Toumpourleka, Georgia Pitsiou, Evaggelia Panagiotidou, Sophia Anastasia Mouratoglou, Georgios Sianos, Stavros Hadjimiltiades, Antonios Pitsis, Eckhard Mayer, Ioannis Stanopoulos, Haralambos Karvounis, George Giannakoulas
BACKGROUND: Chronic thromboembolic pulmonary hypertension (CTEPH) is a rare, distinct pulmonary vascular disease, and, therefore, there is lack of data regarding its clinical presentation, diagnosis and management at a national basis. Our objective was to describe the demographics and management of CTEPH patients in Northern Greece. METHODS: We conducted a retrospective, observational study with a joint collaboration from two pulmonary hypertension expert centers in Greece and included patients diagnosed with CTEPH...
May 17, 2017: Hellenic Journal of Cardiology: HJC, Hellēnikē Kardiologikē Epitheōrēsē
https://www.readbyqxmd.com/read/28524086/dietary-green-pea-protects-against-dss-induced-colitis-in-mice-challenged-with-high-fat-diet
#5
Shima Bibi, Luís Fernando de Sousa Moraes, Noelle Lebow, Mei-Jun Zhu
Obesity is a risk factor for developing inflammatory bowel disease. Pea is unique with its high content of dietary fiber, polyphenolics, and glycoproteins, all of which are known to be health beneficial. We aimed to investigate the impact of green pea (GP) supplementation on the susceptibility of high-fat diet (HFD)-fed mice to dextran sulfate sodium (DSS)-induced colitis. Six-week-old C57BL/6J female mice were fed a 45% HFD or HFD supplemented with 10% GP. After 7-week dietary supplementation, colitis was induced by adding 2...
May 18, 2017: Nutrients
https://www.readbyqxmd.com/read/28511592/cytotoxicity-mutagenicity-and-antimutagenicity-of-the-gentisic-acid-on-htc-cells
#6
Flavia Maria Lima Cavalcante, Igor Vivian Almeida, Elisângela Düsman, Mário Sérgio Mantovani, Veronica Elisa Pimenta Vicentini
Gentisic acid (GA) exhibits antioxidant, anti-inflammatory, and antibiotic activities. This substance can be found in citrus fruits, grapes, olive oil, and peas. Considering that there are few studies in the literature on the toxicity of GA, the present work aimed to investigate its cytotoxic, mutagenic, and antimutagenic activities on HTC cells. GA was diluted in culture medium at the final concentration of 0.08, 0.16, 0.8, 1.6, and 8 μg/mL. The cytotoxicity was determined by the MTT assay and Trypan Blue exclusion method, with methyl methanesulfonate and doxorubicin as positive controls, respectively...
May 16, 2017: Drug and Chemical Toxicology
https://www.readbyqxmd.com/read/28507429/factors-associated-with-the-attitudes-of-oncology-nurses-toward-hospice-care-in-china
#7
Fei-Min Yang, Zhi-Hong Ye, Lei-Wen Tang, Wei-Lan Xiang, Lin-Juan Yan, Min-Li Xiang
OBJECTIVE: To examine factors that are associated with the apprehension levels of oncology nurses toward hospice care. Factors examined in this study included demographics, nursing experience, education levels, title and post, personal experiences, and attitudes toward end-of-life care. METHODS: Questionnaires were provided to nurses (n=201) from three first-tier hospitals in China. A quantitative scale, Professional End-of-life Care Attitude Scale (PEAS), was used to assess personal and professional apprehension levels toward hospice care...
2017: Patient Preference and Adherence
https://www.readbyqxmd.com/read/28504581/bacillus-coagulans-gbi-30-6086-increases-plant-protein-digestion-in-a-dynamic-computer-controlled-in-vitro-model-of-the-small-intestine-tim-1
#8
D Keller, R Van Dinter, H Cash, S Farmer, K Venema
The aim of this study was to assess the potential of the probiotic Bacillus coagulans GBI-30, 6086 [GanedenBC(30)] (BC30) to aid in protein digestion of alimentary plant proteins. To test this, three plant proteins, from pea, soy and rice, were digested in a validated in vitro model of the stomach and small intestine (TIM-1) in the absence and in the presence of BC30. Samples were taken from the TIM-1 fractions that mimic uptake of amino acids by the host and analysed for α-amino nitrogen (AAN) and total nitrogen (TN)...
May 15, 2017: Beneficial Microbes
https://www.readbyqxmd.com/read/28503950/the-effects-of-rapeseed-meal-and-legume-seeds-as-substitutes-for-soybean-meal-on-productivity-and-gastrointestinal-function-in-rabbits
#9
Andrzej Gugołek, Jerzy Juśkiewicz, Janusz Strychalski, Cezary Zwoliński, Ewa Żary-Sikorska, Małgorzata Konstantynowicz
The aim of this study was to determine the effects of soybean meal (SBM) substitution by a mixture of rapeseed meal (RSM), white lupine seeds (WLS) and pea seeds (PS) on productivity, nutrient digestibility, nitrogen retention and gastrointestinal function in Hyplus rabbits. The Control diet (SBM15) contained 15% SBM, whereas Diet SBM7.5 contained 7.5% SBM, 5% RSM, 4% WLS and 3% PS. In Diet SBM0, SBM was completely replaced by RSM, WLS and PS (10%, 8% and 6%, respectively). A production trial was performed on 90 Hyplus rabbits aged from 35 to 84 d (45 each sex; 953 ± 4...
May 15, 2017: Archives of Animal Nutrition
https://www.readbyqxmd.com/read/28503311/a-community-resource-for-exploring-and-utilizing-genetic-diversity-in-the-usda-pea-single-plant-plus-collection
#10
William L Holdsworth, Elodie Gazave, Peng Cheng, James R Myers, Michael A Gore, Clarice J Coyne, Rebecca J McGee, Michael Mazourek
Globally, pea (Pisum sativum L.) is an important temperate legume crop for food, feed and fodder, and many breeding programs develop cultivars adapted to these end-uses. In order to assist pea development efforts, we assembled the USDA Pea Single Plant Plus Collection (PSPPC), which contains 431 P. sativum accessions with morphological, geographic and taxonomic diversity. The collection was characterized genetically in order to maximize its value for trait mapping and genomics-assisted breeding. To that end, we used genotyping-by-sequencing-a cost-effective method for de novo single-nucleotide polymorphism (SNP) marker discovery-to generate 66 591 high-quality SNPs...
2017: Horticulture Research
https://www.readbyqxmd.com/read/28503053/pea-pisum-sativum-and-its-anticancer-activity
#11
REVIEW
Runchana Rungruangmaitree, Wannee Jiraungkoorskul
Pisum sativum (Family: Fabaceae), as known as green pea or garden pea, has long been important in diet due to its content of fiber, protein, starch, trace elements, and many phytochemical substances. It has been shown to possess antibacterial, antidiabetic, antifungal, anti-inflammatory, antihypercholesterolemia, and antioxidant activities and also shown anticancer property. Its nonnutritive biologically active components include alkaloids, flavonoids, glycosides, isoflavones, phenols, phytosterols, phytic acid, protease inhibitors, saponins, and tannins...
January 2017: Pharmacognosy Reviews
https://www.readbyqxmd.com/read/28501802/stability-of-proteins-in-dried-blood-spot-biobanks
#12
Johan Björkesten, Stefan Enroth, Qiujin Shen, Lotta Wik, David Hougaard, Arieh Cohen, Lene Sörensen, Vilmantas Giedraitis, Martin Ingelsson, Anders Larsson, Masood Kamali-Moghaddam, Ulf Landegren
An important motivation for the construction of biobanks is to discover biomarkers that identify diseases at early, potentially curable stages. This will require biobanks from large numbers of individuals, preferably sampled repeatedly, where the samples are collected and stored under conditions that preserve potential biomarkers. Dried blood samples are attractive for biobanking because of the ease and low cost of collection and storage. Here we have investigated their suitability for protein measurements...
May 13, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28499040/evolving-gene-banks-improving-diverse-populations-of-crop-and-exotic-germplasm-with-optimal-contribution-selection
#13
W A Cowling, L Li, K H M Siddique, M Henryon, P Berg, R G Banks, B P Kinghorn
We simulated pre-breeding in evolving gene banks - populations of exotic and crop types undergoing optimal contribution selection for long-term genetic gain and management of population genetic diversity. The founder population was based on crosses between elite crop varieties and exotic lines of field pea (Pisum sativum) from the primary genepool, and was subjected to 30 cycles of recurrent selection for an economic index composed of four traits with low heritability: black spot resistance, flowering time and stem strength (measured on single plants), and grain yield (measured on whole plots)...
April 1, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28495479/limited-sampling-strategies-for-determining-the-area-under-the-plasma-concentration-time-curve-for-isoniazid-might-be-a-valuable-approach-for-optimizing-treatment-in-adult-patients-with-tuberculosis
#14
Piergiorgio Cojutti, Manuela Giangreco, Miriam Isola, Federico Pea
This study aimed to develop clinically feasible models of limited sampling strategy (LSS) for estimation of the area under the concentration-time curve (AUC24h) for isoniazid, that could be applied easily in daily clinical practice for dosage adjustment in adult patients with tuberculosis. Isoniazid plasma concentrations (n=1665) from 185 adult tuberculous patients were used for the development and validation of LSS models to estimate AUC24h following administration of the standard 5 mg/kg dose of isoniazid...
May 8, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28492347/the-soy-derived-peptide-vglycin-inhibits-the-growth-of-colon-cancer-cells-in%C3%A2-vitro-and-in%C3%A2-vivo
#15
Chang Gao, Rui Sun, Ya-Rong Xie, An-Li Jiang, Mei Lin, Min Li, Zheng-Wang Chen, Ping Zhang, Honglin Jin, Jue-Ping Feng
Vglycin, a novel natural polypeptide isolated from pea seeds, possesses antidiabetic properties. Our previous studies have shown that Vglycin can induce the differentiation of human colon adenocarcinoma cells. We aimed to determine the anticancer activity of Vglycin against colon cancer cells and to elucidate related apoptosis-inducing mechanisms. Treatment with purified Vglycin significantly reduced growth, viability, and colony formation of CT-26, SW480, and NCL-H716 colon cancer cells in a dose-dependent manner while down-regulating the expression of proliferating cell nuclear antigen...
May 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28490655/practice-guideline-summary-reducing-brain-injury-following-cardiopulmonary-resuscitation-report-of-the-guideline-development-dissemination-and-implementation-subcommittee-of-the-american-academy-of-neurology
#16
Romergryko G Geocadin, Eelco Wijdicks, Melissa J Armstrong, Maxwell Damian, Stephan A Mayer, Joseph P Ornato, Alejandro Rabinstein, José I Suarez, Michel T Torbey, Richard M Dubinsky, Jason Lazarou
OBJECTIVE: To assess the evidence and make evidence-based recommendations for acute interventions to reduce brain injury in adult patients who are comatose after successful cardiopulmonary resuscitation. METHODS: Published literature from 1966 to August 29, 2016, was reviewed with evidence-based classification of relevant articles. RESULTS AND RECOMMENDATIONS: For patients who are comatose in whom the initial cardiac rhythm is either pulseless ventricular tachycardia (VT) or ventricular fibrillation (VF) after out-of-hospital cardiac arrest (OHCA), therapeutic hypothermia (TH; 32-34°C for 24 hours) is highly likely to be effective in improving functional neurologic outcome and survival compared with non-TH and should be offered (Level A)...
May 10, 2017: Neurology
https://www.readbyqxmd.com/read/28487704/a-combined-comparative-transcriptomic-metabolomic-and-anatomical-analyses-of-two-key-domestication-traits-pod-dehiscence-and-seed-dormancy-in-pea-pisum-sp
#17
Iveta Hradilová, Oldřich Trněný, Markéta Válková, Monika Cechová, Anna Janská, Lenka Prokešová, Khan Aamir, Nicolas Krezdorn, Björn Rotter, Peter Winter, Rajeev K Varshney, Aleš Soukup, Petr Bednář, Pavel Hanáček, Petr Smýkal
The origin of the agriculture was one of the turning points in human history, and a central part of this was the evolution of new plant forms, domesticated crops. Seed dispersal and germination are two key traits which have been selected to facilitate cultivation and harvesting of crops. The objective of this study was to analyze anatomical structure of seed coat and pod, identify metabolic compounds associated with water-impermeable seed coat and differentially expressed genes involved in pea seed dormancy and pod dehiscence...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28487432/deciphering-mcr-2-colistin-resistance
#18
Jian Sun, Yongchang Xu, Rongsui Gao, Jingxia Lin, Wenhui Wei, Swaminath Srinivas, Defeng Li, Run-Shi Yang, Xing-Ping Li, Xiao-Ping Liao, Ya-Hong Liu, Youjun Feng
Antibiotic resistance is a prevalent problem in public health worldwide. In general, the carbapenem β-lactam antibiotics are considered a final resort against lethal infections by multidrug-resistant bacteria. Colistin is a cationic polypeptide antibiotic and acts as the last line of defense for treatment of carbapenem-resistant bacteria. Very recently, a new plasmid-borne colistin resistance gene, mcr-2, was revealed soon after the discovery of the paradigm gene mcr-1, which has disseminated globally. However, the molecular mechanisms for MCR-2 colistin resistance are poorly understood...
May 9, 2017: MBio
https://www.readbyqxmd.com/read/28482189/the-princess-and-the-pea-effect-influence-of-the-first-layer-on-polyelectrolyte-multilayer-assembly-and-properties
#19
Xuejian Lyu, Amy M Peterson
In many applications of polyelectrolyte multilayers (PEMs), a base polycation layer is adsorbed to promote adhesion of the PEM to the substrate. In this report, the effect of the first polyelectrolyte adsorbed in a PEM was investigated by assembling PEMs with first layer polycations of different chemistries and molecular weights. In this study, quartz crystal microbalance with dissipation monitoring (QCM-D) was used to monitor the PEM assembly process. First layer choice affects the total mass accumulation of the PEM as well as the stoichiometry of the PEM, although linear growth was observed in all cases...
April 29, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28480573/faba-beans-and-peas-in-poultry-feed-economic-assessment
#20
Liga Proskina, Sallija Cerina
BACKGROUND: Broiler diets mainly consist of cereals and protein-rich feed sources; in the EU-27, poultry farming consumes 24% of the total amount of protein-rich feedstuffs. Since the EU produces only 30% of the total quantity of protein crops used for feed, it is necessary to promote the use of traditional European protein crops (beans, peas) for feed in livestock farming. The research aim is to identify economic gains from the production of broiler chicken meat, replacing soybean meal with domestic faba beans and field peas in broiler chicken diets...
May 8, 2017: Journal of the Science of Food and Agriculture
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