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Sabine Wächter, Alexander I Damanakis, Moritz Elxnat, Silvia Roth, Annette Wunderlich, Frederik A Verburg, Sebastian A Fellinger, Detlef K Bartsch, Pietro Di Fazio
Epigenetic modifications have been identified as being responsible for the de-differentiation of thyroid tissue and its malignant transformation. Cell proliferation inhibitory effects of the pan-deacetylase inhibitors panobinostat, SAHA and Trichostatin A (TSA), the modulation of the sodium iodide symporter (NIS; SLC5A5), thyroid transcription factor 1 (TTF1), high mobility group A2 (HMGA2), and H19 and their putative targeting miRNAs have been evaluated in vitro. The cell viability was measured in five thyroid cancer cell lines (FTC133, TPC1, BCPAP, 8505C, C643) by real time cell analyzer xCELLigence...
March 21, 2018: Journal of Clinical Medicine
Marija Nišavić, Goran V Janjić, Amela Hozić, Marijana Petković, Miloš K Milčić, Zoran Vujčić, Mario Cindrić
Binding of three ruthenium(ii) compounds of general formula mer-[Ru(L3)(N-N)X][Y] (where L3 = 4'-chloro-2,2':6',2''-terpyridine (Cl-tpy); N-N = 1,2-diaminoethane (en), 1,2-diaminocyclohexane (dach) or 2,2'-bipyridine (bipy); X = Cl; Y = Cl) to human serum albumin (HSA) has been investigated by nano-LC/nano-ESI MS and docking studies. A bottom-up proteomics approach has been applied for the structural characterization of metallated proteins and the data were analyzed in both the positive and negative ion mode...
March 21, 2018: Metallomics: Integrated Biometal Science
Forough Ghasemi, M Reza Hormozi-Nezhad, Morteza Mahmoudi
Monitoring the ratio of 40- and 42-residue amyloid β peptides (i.e., Aβ40 and Aβ42) in human plasma is considered one of the hallmarks of detection of the early stage of Alzheimer's disease (AD). Therefore, development of a specific, yet non-antibody-based method for simultaneous detection of Aβ40 and Aβ42 may have considerable clinical applications. Here, we developed a 'nanoparticle-based colorimetric sensor array' utilizing label-free gold and silver nanoparticles for visual detection of Aβ42 and Aβ40...
March 21, 2018: Nanoscale
Damir Klepac, Hana Kostková, Svetlana Petrova, Petr Chytil, Tomáš Etrych, Sami Kereïche, Ivan Raška, David A Weitz, Sergey K Filippov
In this paper, we revised the current understanding of the protein corona that is created on the surface of nanoparticles in blood plasma after an intravenous injection. We have focused on nanoparticles that have a proven therapeutic outcome. These nanoparticles are based on two types of biocompatible amphiphilic copolymers based on N-(2-hydroxypropyl)methacrylamide (HPMA): a block copolymer, poly(ε-caprolactone) (PCL)-b-poly(HPMA), and a statistical HPMA copolymer bearing cholesterol moieties, which have been tested both in vitro and in vivo...
March 21, 2018: Nanoscale
R Ravikanth Reddy, Ganesh Shanmugam, Balaraman Madhan, B V N Phani Kumar
The interaction of ionic liquid (IL) with protein is now becoming important as it stabilizes the protein due to the selective cation-anion combination of the IL. The binding and dynamics of the green solvents such as imidazole alkyl sulfate based ILs, viz., 1-butyl-3-methylimidazolium alkyl [where alkyl = hydrogen, methyl, octyl and dodecyl] sulfate, with two distinct model proteins, namely human serum albumin (HSA) and collagen in aqueous solution, have been investigated with the aid of solution nuclear magnetic resonance (NMR)...
March 21, 2018: Physical Chemistry Chemical Physics: PCCP
Sara Pagotto, Angelo Veronese, Alessandra Soranno, Paola Lanuti, Mirco Di Marco, Marco Vincenzo Russo, Alice Ramassone, Marco Marchisio, Pasquale Simeone, Paolo E Guanciali Franchi, Giandomenico Palka, Renato Mariani Costantini, Carlo M Croce, Rosa Visone
Hsa-miR-155-5p (miR-155) is overexpressed in most solid and hematological malignancies. It promotes loss of genomic integrity in cancer cells by targeting genes involved in microsatellite instability and DNA repair; however, the link between miR-155 and aneuploidy has been scarcely investigated. Here we describe a novel mechanism by which miR-155 causes chromosomal instability. Using osteosarcoma cells (U2OS) and normal human dermal fibroblast (HDF), two well-established models for the study of chromosome congression, we demonstrate that miR-155 targets the spindle checkpoint proteins BUB1, CENP-F, and ZW10, thus compromising chromosome alignment at the metaphase plate...
February 27, 2018: Oncotarget
Xuejuan Wang, Salar Ahmad, Zhihui Zhang, Jacques Côté, Gang Cai
The NuA4/TIP60 acetyltransferase complex is required for gene regulation, DNA repair and cell cycle progression. The limited structural information impeded understanding of NuA4/TIP60 assembly and regulatory mechanism. Here, we report the 4.7 Å cryo-electron microscopy (cryo-EM) structure of a NuA4/TIP60 TEEAA assembly (Tra1, Eaf1, Eaf5, actin and Arp4) and the 7.6 Å cryo-EM structure of a TEEAA-piccolo assembly (Esa1, Epl1, Yng2 and Eaf6). The Tra1 and Eaf1 constitute the assembly scaffold. The Eaf1 SANT domain tightly binds to the LBE and FATC domains of Tra1 by ionic interactions...
March 20, 2018: Nature Communications
Francois Pognan, J Andreas Mahl, Maria Papoutsi, David Ledieu, Marc Raccuglia, Diethilde Theil, Sarah B Voytek, Patrick J Devine, Katie Kubek-Luck, Natalie Claudio, Andre Cordier, Annabelle Heier, Carine Kolly, Andreas Hartmann, Salah-Dine Chibout, Page Bouchard, Christian Trendelenburg
A high incidence of hemangiosarcoma (HSA) was observed in mice treated for 2 years with siponimod, a sphingosine-1-phosphate receptor 1 (S1P1) functional antagonist, while no such tumors were observed in rats under the same treatment conditions. In 3-month rat (90 mg/kg/day) and 9-month mouse (25 and 75 mg/kg/day) in vivo mechanistic studies, vascular endothelial cell (VEC) activation was observed in both species, but VEC proliferation and persistent increases in circulating placental growth factor 2 (PLGF2) were only seen in the mouse...
March 19, 2018: Archives of Toxicology
Silvia Di Agostino, Fabio Valenti, Andrea Sacconi, Giulia Fontemaggi, Matteo Pallocca, Claudio Pulito, Federica Ganci, Paola Muti, Sabrina Strano, Giovanni Blandino
Over 70% of head & neck squamous cell carcinoma (HNSCC) patients carry TP53 oncogenic mutations. Here we studied the role of specific tumor-derived mutant p53 proteins in the aberrant transcription of long non-coding (lnc) MIR205HG gene in head and neck cancer cells. Methods: To understand the role of lncMIR205HG, that we showed to be transcriptionally regulated by mutant p53 in HNSCC, we have employed siRNA and shRNA in CAL27 and FaDu HNSCC cell lines to suppress p53 gene expression in ChIP assays and RT-qPCR...
2018: Theranostics
Xiaoni Liu, Shuang Wang, Jianji Xu, Buxin Kou, Dexi Chen, Yajie Wang, Xiaoxin Zhu
BACKGROUND: MicroRNAs(miRNAs)are involved in the initiation and progression of hepatocellular carcinoma. ESC, an extract of Stellerachamaejasme L, had been confirmed as a potential anti-tumor extract of Traditional Chinese Medicine. In light of the important role of miRNAs in hepatocellular carcinoma, we questioned whether the inhibitory effects of ESC on hepatocellular carcinoma (HCC) were associated with miRNAs. METHODS: The proliferation inhibition of ESC on HCC cells was measured with MTT assay...
March 20, 2018: BMC Complementary and Alternative Medicine
Jianghong Huang, Yujie Liang, Meiquan Xu, Jianyi Xiong, Daping Wang, Qiang Ding
The aim of the present study was to investigate the clinical significance of hsa-microRNA-124-3p (miR-124) in osteosarcoma (OS), and examine its role in cell growth and invasion. Using a microRNA chip array, the expression of miR-124 was detected in samples of surgically resected OS and matched against the levels of expression in tumor-adjacent normal tissues. The levels of miR-124 were upregulated in the OS cells through the transfection of miR-124 mimics. Cell proliferation and Transwell assays were performed to determine cell proliferation and invasion; Reverse transcription-quantitative polymerase chain reaction, western blot and luciferase assays were then used to detect the expression of the target gene snail family zinc finger 2 (Snail2)...
April 2018: Oncology Letters
Mousumi Sahu, Bibekanand Mallick
MicroRNAs (miRNAs) play crucial roles in pluripotency and differentiation of human Embryonic Stem Cells (hESCs). However, synergism among multiple miRNAs and their regulatory effects on stem cells are largely unknown. We investigated the synergistic regulations among miRNAs, which are differentially expressed in hESCs and fibroblasts (Fibs) to gain deeper insights into the regulatory mechanisms of miRNA-miRNA synergism in differentiation of hESCs into Fibs. In this study, we identified miRNA targets incorporating differential expression profiles of miRNAs and genes in hESCs-Fibs as well as miRNA targeting information followed by enrichment analysis...
March 14, 2018: International Journal of Biological Macromolecules
Xingjia Guo, Jie Yao, Xuehui Liu, Hongyan Wang, Lizhi Zhang, Liping Xu, Aijun Hao
Eu3+ doped LaPO4 fluorescent nanorods (LaPO4 :Eu) was successfully fabricated by a hydrothermal process. The obtained LaPO4 :Eu nanorods under the optimal conditions were characterized by means of transmission electron microscopy (TEM), X-ray diffraction (XRD) technique, Fourier transform infrared (FTIR), UV-vis absorption and fluorescence spectroscopy. The nanorods with a length of 50-100nm and a diameter of about 10nm, can emit strong red fluorescence upon excitation at 241nm. The FTIR result confirmed that there are lots of phosphate groups on the surfaces of nanorods...
March 6, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Qiyu Lu, Yi Sun, Yuyin Duan, Bin Li, Jianming Xia, Songhua Yu, Guimin Zhang
BACKGROUND: Valvular heart disease is a leading cause of cardiovascular mortality, especially in China. More than a half of valvular heart diseases are caused by acute rheumatic fever. microRNA is involved in many physiological and pathological processes. However, the miRNA profile of the rheumatic valvular heart disease is unknown. This research is to discuss microRNAs and their target gene pathways involved in rheumatic heart valve disease. METHODS: Serum miRNA from one healthy individual and four rheumatic heart disease patients were sequenced...
March 16, 2018: BMC Cardiovascular Disorders
Jing Chen, Yan Qi, Cui-Fang Liu, Jing-Min Lu, Yan Shi
INTRODUCTION: MicroRNAs have been increasing prevalent due to the association of their deregulation with neurodegenerative disorders, especially Alzheimer's disease (AD). However, the association between miRNAs and AD hasn't been made clear. PURPOSE: In this study, Nine representative miRNA datasets were selected for the identification of the critical miRNAs by analyzing the overlap relations among them. TargetScan software was further used to predict the target genes of these miRNAs...
March 15, 2018: Journal of Gene Medicine
Tiziana Alberio, Greta Forlani, Marta Lualdi, Giovanna Tosi, Roberto S Accolla, Mauro Fasano
BACKGROUND: Fibrinogen is a central player in the blood coagulation cascade and one of the most abundant plasma proteins. This glycoprotein also triggers important events (e.g., cell spreading, the respiratory burst and degranulation) in neutrophil cells via a αM β2 integrin-mediated binding to the cell surface. Yet, little is known about the interaction of fibrinogen with leukocytes other than neutrophils or stimulated monocytes, although high amounts of fibrinogen protein can also be found in lymphocytes, particularly in T-cells...
March 14, 2018: Journal of Translational Medicine
Hasmik Grigoryan, William M B Edmands, Qing Lan, Henrik Carlsson, Roel Vermeulen, Luoping Zhang, Song-Nian Yin, Gui-Lan Li, Martyn T Smith, Nathaniel Rothman, Stephen M Rappaport
Although benzene has long been recognized as a cause of human leukemia, the mechanism by which this simple molecule causes cancer has been problematic. A complicating factor is benzene metabolism, which produces many reactive intermediates, some specific to benzene and others derived from redox processes. Using archived serum from 20 nonsmoking Chinese workers, 10 with and 10 without occupational exposure to benzene (exposed: 3.2 - 88.9 ppm, controls: 0.002 - 0.020 ppm), we employed an adductomic pipeline to characterize protein modifications at Cys34 of human serum albumin (HSA), a nucleophilic hotspot in extracellular fluids...
March 10, 2018: Carcinogenesis
Hong Shen, Shuyang Lu, Lili Dong, Yuan Xue, Chenling Yao, Chaoyang Tong, Chunsheng Wang, Xianhong Shu
Abnormal expression of microRNAs (miRNAs) has been associated with aortic dissection (AD). Next generation sequencing was performed to identify the differentially expressed miRNAs in aortic tissue samples between AD and non-diseased individuals. Selected miRNAs which showed significant variation between the two groups, were then transfected into human aortic vascular smooth muscle cells (HA-VSMC), and assessed for effects on cell migration and induced apoptosis. The changes in gene expression pattern in HA-VSMC cells transfected with the miRNAs were also investigated...
March 9, 2018: Journal of Cardiovascular Pharmacology
E M Kithsiri Wijeratne, Maria C F Oliveira, Jair Mafezoli, Ya-Ming Xu, Sandro Minguzzi, Pedro H J Batista, Otília D L Pessoa, Luke Whitesell, A A Leslie Gunatilaka
Withanolides constitute a valuable class of bioactive natural products because some members of the class are known to exhibit cytotoxic activity and also induce a cytoprotective heat-shock response. In order to understand the relationship between their structures and these dual bioactivities of the withanolide scaffold, we obtained 25 analogues of withaferin A (WA) and withanolide D (WD) including 17 new compounds by semisynthesis involving chemical and microbial transformations. Hitherto unknown 16β-hydroxy analogues of WA and WD were prepared by their reaction with triphenylphosphine/iodine, providing unexpected 5β-hydroxy-6α-iodo analogues (iodohydrins) followed by microbial biotransformation with Cunninghamella echinulata and base-catalyzed cyclization of the resulting 16β-hydroxy iodohydrins...
March 14, 2018: Journal of Natural Products
Yi Wang, Huaiyi Huang, Ge Chen, Haijie Chen, Tingting Xu, Qian Tang, Hailiang Zhu, Qianling Zhang, Pingyu Zhang
We report a novel organo-iridium(iii) complex (Ir1) for the selective and sensitive determination of human serum albumin (HSA). The phosphorescence of Ir1 is turned-on by HSA in the urinary system. Importantly, Ir1 is much simpler and less time-consuming for HSA staining than Coomassie blue in proteomics studies.
March 14, 2018: Chemical Communications: Chem Comm
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