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https://www.readbyqxmd.com/read/28644884/ligand-induced-rapid-skeletal-muscle-atrophy-in-hsa-fv2e-perk-transgenic-mice
#1
Masato Miyake, Masashi Kuroda, Hiroshi Kiyonari, Kenji Takehana, Satoshi Hisanaga, Masatoshi Morimoto, Jun Zhang, Miho Oyadomari, Hiroshi Sakaue, Seiichi Oyadomari
BACKGROUND: Formation of 43S and 48S preinitiation complexes plays an important role in muscle protein synthesis. There is no muscle-wasting mouse model caused by a repressed 43S preinitiation complex assembly. OBJECTIVE: The aim of the present study was to develop a convenient mouse model of skeletal muscle wasting with repressed 43S preinitiation complex assembly. MATERIAL AND METHODS: A ligand-activatable PERK derivative Fv2E-PERK causes the phosphorylation of eukaryotic initiation factor 2α (eIF2α), which inhibits 43S preinitiation complex assembly...
2017: PloS One
https://www.readbyqxmd.com/read/28644614/role-of-the-protein-corona-derived-from-human-plasma-in-cellular-interactions-between-nanoporous-human-serum-albumin-particles-and-endothelial-cells
#2
Mikhail Valeryevich Zyuzin, Yan Yan, Raimo Hartmann, Katelyn T Gause, Moritz Nazarenus, Jiwei Cui, Frank Caruso, Wolfgang J Parak
The presence of a protein corona on various synthetic nanomaterials has been shown to strongly influence how they interact with cells. However, it is unclear if the protein corona also exists on protein particles, and if so, its role in particle-cell interactions. In this study, pure human serum albumin (HSA) particles were fabricated via mesoporous silica particle templating. Our data reveal that various serum proteins adsorbed on the particles. The presence of a corona from human plasma was shown to decrease particle binding to cell membrane, increase the residence time of particles in early endosomes, and reduce the amount of internalized particles within the first hours of cell exposure to particles...
June 23, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28644391/molecular-structure-affinity-relationship-of-flavonoids-in-lotus-leaf-nelumbo-nucifera-gaertn-on-binding-to-human-serum-albumin-and-bovine-serum-albumin-by-spectroscopic-method
#3
Xiaosheng Tang, Ping Tang, Liangliang Liu
Lotus leaf has gained growing popularity as an ingredient in herbal formulations due to its various activities. As main functional components of lotus leaf, the difference in structure of flavonoids affected their binding properties and activities. In this paper, the existence of 11 flavonoids in lotus leaf extract was confirmed by High Performance Liquid Chromatography (HPLC) analysis and 11 flavonoids showed various contents in lotus leaf. The interactions between lotus leaf extract and two kinds of serum albumins (human serum albumin (HSA) and bovine serum albumin (BSA)) were investigated by spectroscopic methods...
June 23, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28641935/fatty-acid-modified-gapmer-antisense-oligonucleotide-and-serum-albumin-constructs-for-pharmacokinetic-modulation
#4
Michael Lykke Hvam, Yunpeng Cai, Frederik Dagnæs-Hansen, Jesper Sejrup Nielsen, Jesper Wengel, Jørgen Kjems, Kenneth A Howard
Delivery technologies are required for realizing the clinical potential of molecular medicines. This work presents an alternative technology to preformulated delivery systems by harnessing the natural transport properties of serum albumin using endogenous binding of gapmer antisense oligonucleotides (ASOs)/albumin constructs. We show by an electrophoretic mobility assay that fatty acid-modified gapmer and human serum albumin (HSA) can self-assemble into constructs that offer favorable pharmacokinetics. The interaction was dependent on fatty acid type (either palmitic or myristic acid), number, and position within the gapmer ASO sequence, as well as phosphorothioate (PS) backbone modifications...
June 19, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/28641554/future-of-spermatogonial-stem-cell-culture-application-of-nanofiber-scaffolds
#5
Azar Shams, Neda Eslahi, Mansoureh Movahedin, Fariborz Izadyar, Hamidreza Asgari, Morteza Koruji
The spermatogonial stem cells (SSCs) are unique in mammals because they can transmit genetic information from generation to generation and it is of significant importance. In testes, Sertoli cells, peritubular myoid cells, Leydig cells and other interstitial cells contribute to the spermatogonial stem cell "niche". So, creation of niche in in vitro condition that mimics in vivo environment is essential to maintain functional characteristic of SSCs. Nanofiber matrices mimic the architecture and size scale of the natural extracellular matrix (ECM)...
June 22, 2017: Current Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28640541/design-and-fabrication-of-dual-targeted-delivery-system-based-on-gemcitabine-conjugated-human-serum-albumin-nanoparticles
#6
Parisa Norouzi, Mohsen Amini, Fatemeh Mottaghitalab, Farnaz Sadat Mirzazadeh Tekie, Rassoul Dinarvand, Zahra Hadavand Mirzaie, Fatemeh Atyabi
Dual-targeted drug delivery system has established their reputation as potent vehicles for cancer chemotherapies. Herein, gemcitabine (Gem) was conjugated to human serum albumin (HSA) via dithiodipropionic anhydride (DTDPA) to fabricate Gem-HSA nanoparticles. It was hypothesized that this system can enhance the low stability of Gem and can improve its intracellular delivery. Furthermore, folate was applied as targeting agent on HSA nanoparticles for increasing the tumor selectivity of Gem. In order to evaluate the structural properties of synthesized products, (1) H NMR and FT-IR were performed...
June 22, 2017: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/28640302/influence-of-substituents-on-dna-and-protein-binding-of-cyclometalated-ir-iii-complexes-and-anticancer-activity
#7
Sujay Mukhopadhyay, Roop Shikha Singh, Rajendra Prasad Paitandi, Gunjan Sharma, Biplob Koch, Daya Shankar Pandey
Synthesis of terpyridyl based ligands 3-([2,2':6',2''-terpyridin]-4'-yl)-7-methoxy-2-(methylthio)-quinolone, (L1); 3-([2,2':6',2''-terpyridin]-4'-yl)-6-methoxyquinolin-2(1H)-one, (L2); 3-([2,2'-:6',2''-terpyridin]-4'-yl)-6-methylquinolin-2(1H)-one (L3) and cyclometalated iridium(iii) complexes [[Ir(ppy)2L1](+)PF6(-) (1), [Ir(ppy)2L2](+)PF6(-) (2), [Ir(ppy)2L3](+)PF6(-) (3) (2-phenylpyridine = Hppy)] involving these ligands has been described. The ligands L1-L3 and complexes 1-3 have been thoroughly characterized by elemental analyses, spectral studies (IR, (1)H, (13)C NMR, UV/vis and fluorescence) ESI-MS, and the structure of 3 has been unambiguously authenticated by single crystal X-ray analyses...
June 22, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28639908/circular-rnas-play-an-important-role-in-late-stage-gastric-cancer-circular-rna-expression-profiles-and-bioinformatics-analyses
#8
Yantian Fang, Minzhe Ma, Jiangli Wang, Xiaowen Liu, Yanong Wang
Gastric cancer is one of the most common tumors of the digestive system. Here, analysis of the expression profiles of circular RNAs in advanced gastric adenocarcinoma and adjacent normal mucosa tissues revealed differential expression of 306 circular RNAs, among which 273 were predicted to exert regulatory effects on target microRNAs. The downstream pathway networks of circular RNA-microRNA were mapped and the node genes were identified. In particular, we found that the expression of hsa_circ_0058246 was elevated in tumor specimens of patients with poor clinical outcomes...
June 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28639886/a-competing-endogenous-rna-network-identifies-novel-mrna-mirna-and-lncrna-markers-for-the-prognosis-of-diabetic-pancreatic-cancer
#9
Kanyu Yao, Qi Wang, Jianhua Jia, Haiping Zhao
Pancreatic cancer (PaC) is highly associated with diabetes mellitus (DM). However, the mechanisms are insufficient. The study aimed to uncover the underlying regulatory mechanism on diabetic PaC and find novel biomarkers for the disease prognosis. Two RNA-sequencing (RNA-seq) datasets, GSE74629 and GSE15932, as well as relevant data in TCGA were utilized. After pretreatment, differentially expressed genes (DEGs) or miRNAs (DEMs) or lncRNAs (DELs) between diabetic PaC and non-diabetic PaC patients were identified, and further examined for their correlations with clinical information...
June 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28638483/thermoacoustic-imaging-and-therapy-guidance-based-on-ultra-short-pulsed-microwave-pumped-thermoelastic-effect-induced-with-superparamagnetic-iron-oxide-nanoparticles
#10
Liewei Wen, Sihua Yang, Junping Zhong, Quan Zhou, Da Xing
Multifunctional nanoparticle-mediated imaging and therapeutic techniques are promising modalities for accurate localization and targeted treatment of cancer in clinical settings. Thermoacoustic (TA) imaging is highly sensitive to detect the distribution of water, ions or specific nanoprobes and provides excellent resolution, good contrast and superior tissue penetrability. TA therapy is a potential non-invasive approach for the treatment of deep-seated tumors. In this study, human serum albumin (HSA)-functionalized superparamagnetic iron oxide nanoparticle (HSA-SPIO) is used as a multifunctional nanoprobe with clinical application potential for MRI, TA imaging and treatment of tumor...
2017: Theranostics
https://www.readbyqxmd.com/read/28638147/sensitivity-of-human-pluripotent-stem-cells-to-insulin-precipitation-induced-by-peristaltic-pump-based-medium-circulation-considerations-on-process-development
#11
Diana Massai, Emiliano Bolesani, Diana Robles Diaz, Christina Kropp, Henning Kempf, Caroline Halloin, Ulrich Martin, Tudor Braniste, Giuseppe Isu, Vanessa Harms, Umberto Morbiducci, Gerald Dräger, Robert Zweigerdt
Controlled large-scale production of human pluripotent stem cells (hPSCs) is indispensable for their envisioned clinical translation. Aiming at advanced process development in suspension culture, the sensitivity of hPSC media to continuous peristaltic pump-based circulation, a well-established technology extensively used in hydraulically-driven bioreactors, was investigated. Unexpectedly, conditioning of low protein media (i.e. E8 and TeSR-E8) in a peristaltic pump circuit induced severe viability loss of hPSCs cultured as aggregates in suspension...
June 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28637446/mir-363-5p-as-potential-prognostic-marker-for-hepatocellular-carcinoma-indicated-by-weighted-co-expression-network-analysis-of-mirnas-and-mrna
#12
Jun Zhang, Jia Fan, Chongming Zhou, Yanyu Qi
BACKGROUND: This study aimed to investigate potential miRNAs and genes associated with the prognosis of hepatocellular carcinoma (HCC). METHODS: Weighted co-expression network analysis was utilized to analyze the mRNA and miRNA sequencing data of HCC from TCGA (The Cancer Genome Atlas) database. Significant network modules were identified, and then functions of genes in the gene network modules and target genes of miRNAs in the miRNA network modules were explored...
June 20, 2017: BMC Gastroenterology
https://www.readbyqxmd.com/read/28632276/optimising-the-relaxivities-of-mn-2-complexes-by-targeting-human-serum-albumin-hsa
#13
Attila Forgács, Lorenzo Tei, Zsolt Baranyai, David Esteban-Gómez, Carlos Platas-Iglesias, Mauro Botta
We report two novel macrocyclic ligands based on the 1,4-DO2AM platform (1,4-DO2AM = 2,2'-(1,4,7,10-tetraazacyclododecane-1,4-diyl)diacetamide) and containing two benzyl groups attached either to the nitrogen atoms of the macrocyclic unit (1,4-BzDO2AM) or to the amide pendant arms (1,4-DO2AMBz). The protonation constants of the ligands and the stability constants of their Mn(2+) complexes were determined using pH potentiometry. The introduction of benzyl groups results in a slight decrease of the stability constants of the Mn(2+) complexes and a slight increase of their acid-catalysed dissociation reactions...
June 20, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28631567/microrna-1285-5p-influences-the-proliferation-and-metastasis-of-non-small-cell-lung-carcinoma-cells-via-downregulating-cdh1-and-smad4
#14
Shixia Zhou, Zhongmian Zhang, Pengyuan Zheng, Wenchao Zhao, Na Han
Abnormal expression of microRNAs has been reported to regulate gene expression and cancer cell growth, invasion, and migration. Recently, upregulation of hsa-miR-1285 was demonstrated in bronchoalveolar lavage fluid samples from patients with lung cancer and downregulation in plasma level of stage-I lung cancer patients. However, the function and the underlying mechanism of miR-1285 in non-small-cell lung carcinoma have not been elucidated. In this study, we found that miR-1285-5p, the mature form of miR-1285, was significantly upregulated in human non-small-cell lung carcinoma cell lines A549 and SK-MES-1...
June 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28631291/folate-deficiency-disturbs-hsa-let-7-g-level-through-methylation-regulation-in-neural-tube-defects
#15
Li Wang, Shaofang Shangguan, Yu Xin, Shaoyan Chang, Zhen Wang, Xiaolin Lu, Lihua Wu, Bo Niu, Ting Zhang
Folic acid deficiency during pregnancy is believed to be a high-risk factor for neural tube defects (NTDs). Disturbed epigenetic modifications, including miRNA regulation, have been linked to the pathogenesis of NTDs in those with folate deficiency. However, the mechanism by which folic acid-regulated miRNA influences this pathogenesis remains unclear. It is believed that DNA methylation is associated with dysregulated miRNA expression. To clarify this issue, here we measured the methylation changes of 22 miRNAs in 57 human NTD cases to explore whether such changes are involved in miRNA regulation in NTD cases through folate metabolism...
June 19, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28629862/sustained-delivery-of-bsa-hsa-from-biocompatible-plant-cellulose-nanocrystals-for-in-vitro-cholesterol-release-from-endothelial-cells
#16
Rubbel Singla, Sourabh Soni, Yogendra S Padwad, Amitabha Acharya, Sudesh Kumar Yadav
Nanocomposites of plant cellulose nanocrystals (CNCs) were developed by binding model proteins BSA and HSA onto CNCs by physical adsorption and chemical conjugation methods The spectroscopy and microscopy studies confirmed the protein binding onto CNCs. Phosphate buffer saline (pH=4.0, 7.4) and simulated gastric and intestinal fluids (SGF/SIF; pH=1.1/6.5) showed maximum protein release of ∼62% over a period of time. The released proteins were found to retain both structural integrity as well as≥90% of bioactivity...
June 16, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28626167/a-human-glucagon-like-peptide-1-albumin-recombinant-protein-with-prolonged-hypoglycemic-effect-provides-efficient-and-beneficial-control-of-glucose-metabolism-in-diabetic-mice
#17
Caina Li, Miaomiao Yang, Guojiang Hou, Shuainan Liu, Yi Huan, Dongan Yu, Sujuan Sun, Quan Liu, Shousheng Yan, Zhufang Shen
GW002 is a recombinant protein engineered by fusing the C-terminal region of human glucagon-like peptide-1 (GLP-1) to the N-terminal region of human serum albumin (HSA) with a peptide linker. This study aims to evaluate its anti-diabetic effects both in vitro and vivo. The GLP-1 receptor-dependent luciferase reporter plasmid was transiently transfected in NIT-1 cells to calculate the half-maximal concentration (EC50) for GLP-1 receptor activation, and normal ICR mice and diabetic KKAy mice were acutely injected with GW002 (1, 3 and 9 mg/kg) subcutaneously to evaluate the hypoglycemic action, while the diabetic KKAy and db/db mice were treated with GW002 once daily for 7 weeks to evaluate the effects on glucose metabolism...
June 17, 2017: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28624351/inhibitory-effect-of-vitamin-b3-against-glycation-and-reactive-oxygen-species-production-in-hsa-an-in%C3%A2-vitro-approach
#18
K M Abdullah, Faizan Abul Qais, Iqbal Ahmad, Imrana Naseem
Hyperglycaemia is a key factor for the formation of advanced glycated endproducts (AGEs). Inhibition of glycation may play key role in minimizing the diabetes related complications. We have tried to explore the glucose and methyl glyoxal mediated glycation and antiglycation activity of niacin using human serum albumin as model protein. Protein was incubated with glucose for 28 days at physiological temperature to achieve glycation. Antiglycation activity was evaluated by assessing free lysine, carbonyl content, AGE specific fluorescence...
June 15, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28623731/new-cu-ii-complexes-with-pyrazolyl-derived-schiff-base-ligands-synthesis-and-biological-evaluation
#19
Nádia Ribeiro, Somnath Roy, Nataliya Butenko, Isabel Cavaco, Teresa Pinheiro, Irina Alho, Fernanda Marques, Fernando Avecilla, João Costa Pessoa, Isabel Correia
Since the discovery of cisplatin there has been a continuous pursuit for new metallodrugs showing higher efficacies and lower side effects. In this work, new copper(II) complexes (C1-C6) of Schiff bases derived from pyrazolyl were developed. Through condensation of 5-methyl-1H-pyrazole-3-carbohydrazide with different aromatic aldehydes - pyridoxal, salicylaldehyde, 3-methoxy-2-hydroxybenzaldehyde, 3-ethoxy-2-hydroxybenzaldehyde and 2-hydroxynaphthene-1-carbaldehyde - a set of new pyrazole based "ONO" tridentate Schiff bases were obtained in moderate to good yields - L1-L6, as well as their Cu(II)-complexes...
June 1, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28623723/dft-b3lyp-calculations-in-vitro-cytotoxicity-and-antioxidant-activities-of-steroidal-pyrimidines-and-their-interaction-with-hsa-using-molecular-docking-and-multispectroscopic-techniques
#20
Abad Ali, Mohd Asif, Parvez Alam, Mohammad Jane Alam, Mohd Asif Sherwani, Rizwan Hasan Khan, Shabbir Ahmad, Shamsuzzaman
As a part of our continuing program on the synthesis of steroidal heterocycles, it has been prepared a series of novel steroidal pyrimidine derivatives 4-6via TMSCl, steroidal ketones (1c-3c), urea and benzaldehyde. The systems presented here, are novel scaffolds and have not been described before at 6th position of steroidal-6-one (1c-3c). Structural assignment of newly synthesized compounds was performed by DFT/B3LYP calculations as well as spectral and analytical data. The interactions of compounds (4-6) with HSA were studied by fluorescence spectroscopy, DLS, CD and molecular docking, under imitated physiological conditions...
June 14, 2017: Bioorganic Chemistry
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