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https://www.readbyqxmd.com/read/29049992/iridoids-with-genipin-stem-nucleus-inhibit-lipopolysaccharide-induced-inflammation-and-oxidative-stress-by-blocking-the-nf-%C3%AE%C2%BAb-pathway-in-polycystic-ovary-syndrome
#1
Tao Zuo, Minghui Zhu, Wenming Xu, Zhixia Wang, Hang Song
BACKGROUND/AIMS: Polycystic ovary syndrome (PCOS) is a common endocrine disorder in women, and it is usually characterized by chronic inflammation, oxidative stress, and altered microRNA expression. The aim of this study is to investigate how the effects of iridoids with genipin stem nucleus inhibit PCOS complications. The interactions between iridoids were investigated, as well. METHODS: The chronic inflammation cell model was induced using lipopolysaccharide (LPS) in the RAW 264...
October 19, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29049941/induced-structural-changes-of-humic-acid-by-exposure-of-polystyrene-microplastics-a-spectroscopic-insight
#2
Wei Chen, Zhen-Yu Ouyang, Chen Qian, Han-Qing Yu
The occurrence of microplastics (MPs) as emerging contaminants in the environment may cause changes in water or sediment characteristics, and further affect their biogeochemical cycles. Thus, insights into the interactions between dissolved organic matter (DOM) and MPs are essential for the assessment of environmental impacts of MPs in ecosystems. Integrating spectroscopic methods with chemometric analyses, this work explored the chemical and microstructural changes of DOM-MP complex to reveal the mechanism of DOM-MP interaction at a molecular level...
October 16, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/29049729/myocilin-regulates-metalloprotease-2-activity-through-interaction-with-timp3
#3
Myung Kuk Joe, Raquel L Lieberman, Naoki Nakaya, Stanislav I Tomarev
Purpose: To elucidate functions of wild-type myocilin, a secreted glycoprotein associated with glaucoma. Methods: Lysates of mouse eyes were used for immunoprecipitation with affinity-purified antibodies against mouse myocilin. Shotgun proteomic analysis was used for the identification of proteins interacting with myocilin. Colocalization of myocilin and tissue inhibitor of metalloproteinases 3 (TIMP3) in different eye structures was investigated by a multiplex fluorescent in situ hybridization and immunofluorescent labeling with subsequent confocal microscopy...
October 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29049542/nanoparticle-optimization-for-enhanced-targeted-anti-cancer-drug-delivery
#4
Ibrahim Chamseddine, Michael Kokkolaras
Nanoparticle-based drug delivery is a promising method to increase the therapeutic index of anti-cancer agents with low median toxic dose. The delivery efficiency, corresponding to the fraction of the injected nanoparticles that adhere to the tumor site, depends on nanoparticle size $a$ and aspect ratio $AR$. Currently, values for these variables are chosen empirically, and may not yield optimal targeted drug delivery. This study applies rigorous optimization to the design of nanoparticles. A preliminary investigation revealed that delivery efficiency increases monotonically with $a$ and $AR$...
October 16, 2017: Journal of Biomechanical Engineering
https://www.readbyqxmd.com/read/29049122/case-series-of-successful-postoperative-pain-management-in-buprenorphine-maintenance-therapy-patients
#5
Barbara L Leighton, Lara W Crock
Buprenorphine maintenance therapy patients frequently have severe postoperative pain due to buprenorphine-induced hyperalgesia and provider use of opioids with limited efficacy in the presence of buprenorphine. The authors report good-to-excellent pain management in 4 obstetric patients using nonopioid analgesics, regional anesthesia, continuation of buprenorphine, and use of opioids with high μ receptor affinity.
November 2017: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/29048832/-preparation-of-cysteine-click-maltose-modified-silica-as-a-hydrophilic-interaction-liquid-chromatography-material-for-the-enrichment-of-glycopeptides
#6
Xudong Sun, Lingyi Zhang, Weibing Zhang
Because of the low abundance of glycoprotein and glycopeptide in complex biological samples, it is urgent to develop an efficient method for glycopeptide enrichment in comprehensive and in-depth glycoproteomes research. Herein, a novel hydrophilic silica was developed through surface modification with cysteine-click maltose (Cys-Mal@SiO2). The developed hydrophilic silica was packed into a solid phase extraction (SPE) column, and applied to the highly selective enrichment and identification of N-linked glycopeptides...
July 8, 2017: Se Pu, Chinese Journal of Chromatography
https://www.readbyqxmd.com/read/29048401/p53-dynamics-orchestrates-with-binding-affinity-to-target-genes-for-cell-fate-decision
#7
Mengqiu Wu, Hui Ye, Zhiyuan Tang, Chang Shao, Gaoyuan Lu, Baoqiang Chen, Yuyu Yang, Guangji Wang, Haiping Hao
Emerging evidence support that temporal dynamics is pivotal for signaling molecules in orchestrating smart responses to diverse stimuli. p53 is such a signaling molecule that employs temporal dynamics for the selective activation of downstream target genes and ultimately for cell fate decision. Yet how this fine-tuned p53 machinery is quantitatively decoded remains largely unclear. Here we report a quantitative mechanism defining how p53 dynamics orchestrates with binding affinity to target genes for cell fate decision...
October 19, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/29048372/%C3%AE-synuclein-regulates-neuronal-cholesterol-efflux
#8
Jen-Hsiang T Hsiao, Glenda M Halliday, Woojin Scott Kim
α-Synuclein is a neuronal protein that is at the center of focus in understanding the etiology of a group of neurodegenerative diseases called α-synucleinopathies, which includes Parkinson's disease (PD). Despite much research, the exact physiological function of α-synuclein is still unclear. α-Synuclein has similar biophysical properties as apolipoproteins and other lipid-binding proteins and has a high affinity for cholesterol. These properties suggest a possible role for α-synuclein as a lipid acceptor mediating cholesterol efflux (the process of removing cholesterol out of cells)...
October 19, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29048160/destruxin-a-induces-and-binds-hsps-in-bombyx-mori-bm12-cells
#9
Huanhuan Zhang, Weina Hu, Miaomiao Xiao, Shiyi Ou, Hu Qiongbo
Destruxin A (DA) is a cyclodepsipeptidic mycotoxin isolated from the entomopathogenic fungus, Metarhizium anisopliae. It has insecticidal activity against host insect's innate immunity system, but the molecular mechanism is not elucidated yet. In our previous experiment, four HSPs (heat shock proteins, BmHSP70-3, BmHSP75, BmHSP83 and BmHSCP) were characterized from the specific protein electrophoretic bands of Bombyx mori Bm12 cell line treated with DA in the test of drug affinity responsive target stability (DARTS), which implied that these HSPs might be kinds of DA-affinity proteins, or DA induces them up-regulated expression...
October 19, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29048150/carboxymethylated-dextran-modified-n-heterocyclic-carbene-self-assembled-monolayers-on-gold-for-use-in-surface-plasmon-resonance-biosensing
#10
Zhijun Li, Kim A Munro, Mina Narouz, Bin Hao, Cathleen M Crudden, J Hugh Horton, Hongxia Hao
Surface chemistry is a key enabler for various biosensing applications. Surface plasmon resonance (SPR)-based biosensors routinely employ thiol-based chemistry for the linker layer between gold-coated support surfaces and functional biosensor surfaces. However, there is a growing awareness that such sensor surfaces are prone to oxidation/degradation problems in the presence of oxygen, and previous efforts to improve the stability have shown limited advancements. As an alternative, recent studies employing N-heterocyclic carbene (NHC) self assembled monolayers (SAMs) deposited on gold have shown significant promise in this area...
October 19, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29048135/oligonucleotides-with-cationic-backbone-and-their-hybridization-with-dna-interplay-of-base-pairing-and-electrostatic-attraction
#11
Boris Schmidtgall, Arne Kuepper, Melissa Meng, Tom Grossmann, Christian Ducho
Non-natural oligonucleotides represent important (bio)chemical tools and potential therapeutic agents. Backbone modifications altering hybridization properties and biostability can provide useful analogues. Here, we employ an artificial nucleosyl amino acid (NAA) motif for the synthesis of oligonucleotides containing a backbone decorated with primary amines. An oligo-T sequence of this cationic DNA analogue shows significantly increased affinity for complementary DNA. Notably, hybridization with DNA is still governed by Watson-Crick base pairing...
October 19, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29048075/fluorescence-properties-of-doxorubicin-coupled-carbon-nanocarriers
#12
Najmeh Sadat Hosseini Motlagh, Parviz Parvin, Mitra Refahizadeh, Ali Bavali
The effect of graphene oxide (GO) and nanodiamond (ND) is investigated on the spectral properties of doxorubicin (DOX) fluorescence emissions in the form of (DOX+GO) and (DOX+ND) biomaterials. It is shown that carbon nanostructure additives lead to sensible blueshifts, due to their optical properties and surface functionality. The quenching coefficient KND is obtained to be KND=0.043  (μg/ml)(-1) and KGO=0.342  (μg/ml)(-1) in DOX solutions. In general, Stern-Volmer attests that excited (DOX+GO) strongly quenches with respect to that of (DOX+ND) regarding its privileged bonding affinity...
September 10, 2017: Applied Optics
https://www.readbyqxmd.com/read/29047924/temperature-dependence-of-photoemission-characteristics-from-alxga1-xas-gaas-photocathodes
#13
Kun Wang, Guiyuan Wang, Benkang Chang, Hongcam Tran, Rongguo Fu
In this work, photon-enhanced thermionic emission (PETE), which simultaneously harvests solar photonic and thermionic energies, is studied theoretically and experimentally with a transmission-mode AlxGa1-xAs/GaAs cathode within a compact photodiode. The effect of temperature on energy distribution and photoemission yield was experimentally studied in the wavelength range 450-850 nm. The variation of the energy distribution with increasing temperatures demonstrates that direct photoemission gradually declines, while PETE contribution increases with increased heat...
July 20, 2017: Applied Optics
https://www.readbyqxmd.com/read/29047717/broadening-the-reflection-bandwidth-of-polymer-stabilized-cholesteric-liquid-crystal-via-a-reactive-surface-coating-layer
#14
Jongyoon Kim, Hyungmin Kim, Seongil Kim, Suseok Choi, Wonbong Jang, Jinwuk Kim, Ji-Hoon Lee
We report a method of broadening the reflection bandwidth of polymer-stabilized cholesteric liquid crystal (PSCLC). The top substrate was consecutively coated with a polyimide (PI) and a reactive mesogen (RM) layer, while the bottom substrate was coated with only PI. We exposed the top substrate with the RM coating to UV light. The reflection bandwidth of the PSCLC samples where the top substrate was over-coated with RM was significantly broader than the samples where both substrates were coated with PI. In addition to the effect of the UV intensity gradient, the RM-coated top substrate has a chemical affinity to bulk RM, promoting formation of the pitch gradient and broadening the reflection bandwidth in the sample...
July 10, 2017: Applied Optics
https://www.readbyqxmd.com/read/29047460/computational-insights-into-the-subtype-selectivity-and-message-address-efficacy-mechanisms-of-opioid-receptors-through-jdtic-binding-and-unbinding
#15
Jian-Xin Cheng, Tao Cheng, Wei-Hua Li, Gui-Xia Liu, Wei-Liang Zhu, Yun Tang
In drug design and discovery, binding affinity and selectivity are two basic properties of a drug candidate. Opioid receptors (ORs) are the main targets of strong analgesics. Like some other class A members of G-protein-coupled receptors (GPCRs), ORs exhibit complex selectivity on their ligands. The diversity of binding activity and selectivity among opioids has deeply attracted researchers for a long time. To investigate the subtype selectivity of μ, δ and κ ORs in detail, using the κ-selective antagonist JDTic as a probe, we performed a series of computational simulations, including molecular dynamics and metadynamics, on JDTic-μ/δ/κ-OR complexes...
October 19, 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/29047416/lessons-learned-about-f-18-av-1451-off-target-binding-from-an-autopsy-confirmed-parkinson-s-case
#16
Marta Marquié, Eline E Verwer, Avery C Meltzer, Sally Ji Who Kim, Cinthya Agüero, Jose Gonzalez, Sara J Makaretz, Michael Siao Tick Chong, Prianca Ramanan, Ana C Amaral, Marc D Normandin, Charles R Vanderburg, Stephen N Gomperts, Keith A Johnson, Matthew P Frosch, Teresa Gómez-Isla
[F-18]-AV-1451 is a novel positron emission tomography (PET) tracer with high affinity to neurofibrillary tau pathology in Alzheimer's disease (AD). PET studies have shown increased tracer retention in patients clinically diagnosed with dementia of AD type and mild cognitive impairment in regions that are known to contain tau lesions. In vivo uptake has also consistently been observed in midbrain, basal ganglia and choroid plexus in elderly individuals regardless of their clinical diagnosis, including clinically normal whose brains are not expected to harbor tau pathology in those areas...
October 19, 2017: Acta Neuropathologica Communications
https://www.readbyqxmd.com/read/29047301/pimavanserin-novel-pharmacotherapy-for-parkinson-s-disease-psychosis
#17
Zeyad T Sahli, Frank I Tarazi
Pimavanserin is the first FDA-approved atypical antipsychotic drug indicated for the treatment of hallucinations and delusions associated with Parkinson's disease psychosis (PDP). Areas covered: This review focuses on the preclinical discovery of pimavanserin. It analyzes the pharmacological, behavioral and molecular mechanisms of pimavanserin and their contribution to the therapeutic advantages of the drug as reported in published preclinical and clinical studies, and product labels. Expert opinion: Pimavanserin exhibits a unique pharmacological profile with nanomolar affinity at serotonin 5-HT2A and 5-HT2C receptors...
October 19, 2017: Expert Opinion on Drug Discovery
https://www.readbyqxmd.com/read/29047206/determining-the-cross-talk-between-smooth-muscle-cells-and-macrophages-on-a-cobalt-chromium-stent-material-surface-using-an-in-vitro-post-implantation-co-culture-model
#18
Jordan A Anderson, Sujan Lamichhane, Thomas Vierhout, Daniel Engebretson
Smooth muscle cells (SMCs) and macrophages are important cellular components involved in the development of complications following the implantation of cardiovascular devices. This leads to various disorders such as restenosis, chronic inflammation, and may ultimately result in device failure. In this study, we developed a post-implant stent co-culture model using different ratios of SMCs and macrophages seeded on to cobalt-chromium alloy. The macrophages had an increased affinity to the co-culture surfaces, which resulted in decreased SMC attachment to the alloy surfaces at the initial time point...
October 19, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29047110/cytoskeleton-and-ecm-tumor-mutant-peptides-increased-protease-sensitivities-and-potential-consequences-for-the-hla-class-i-mutant-epitope-reservoir
#19
Blake M Callahan, Jay S Patel, Timothy J Fawcett, George Blanck
Cytoskeleton and extracellular matrix-related proteins (CECMPs) represent the most common class of cancer mutants, owing to the large size of their coding regions and the randomness of mutagenesis. We used a bioinformatics approach to assess the impact of amino acid (AA) substitutions on the sensitivity of CECMPs to proteases relevant to melanoma; and on the binding affinities for HLA class I. CECMP peptides with AA substitutions overwhelmingly reflect increased sensitivity to proteases implicated in melanoma development (MME, CTSS, MMP2, CTSD, CTSL) in comparison to the wild type peptide sequences...
October 19, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/29047027/a-possible-mechanism-for-low-affinity-of-silkworm-na-k-atpase-for-k
#20
Haruo Homareda, Masahiro Otsu, Sachiko Yamamoto, Makoto Ushimaru, Sayaka Ito, Toshiyuki Fukutomi, Taeho Jo, Yoshinobu Eishi, Yukichi Hara
The affinity for K(+) of silkworm nerve Na(+)/K(+)-ATPase is markedly lower than that of mammalian Na(+)/K(+)-ATPase (Homareda 2010). In order to obtain clues on the molecular basis of the difference in K(+) affinities, we cloned cDNAs of silkworm (Bombyx mori) nerve Na(+)/K(+)-ATPase α and β subunits, and analyzed the deduced amino acid sequences. The molecular masses of the α and β subunits were presumed to be 111.5 kDa with ten transmembrane segments and 37.7 kDa with a single transmembrane segment, respectively...
October 19, 2017: Journal of Bioenergetics and Biomembranes
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