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https://www.readbyqxmd.com/read/29236306/rs4705342-polymorphism-is-involved-in-the-tumorigenesis-of-hbv-positive-hcc-by-altering-the-binding-affinity-of-hbv-induced-nf-kb-with-the-promoter-region-of-microrna-143
#1
Yin Xiuli, Sun Shiying, Zhao Junyan, Yang Jing, Lei Xiaofei, Xu Changqing, Li Kun
BACKGROUND: The objective of this study was to explore the role of rs4705342 located in the miR-143 promoter in relation to the control of HBV positive HCC and the underlying molecular mechanism. METHOD: A luciferase assay was performed to explore the factors which influenced miR-143 transcription activity and the target gene of miR-143. This would further be confirmed by ChIP assay. Western-blot and real-time PCR were performed to identify the relationship between miR-143 and ORP8...
December 13, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29236068/in-silico-screening-and-in-vitro-activity-measurement-of-javamide-analogues-as-potential-p38-mapk-inhibitors
#2
Jae B Park
p38 Mitogen-activated protein kinase (p38 MAPK) is a protein kinase critically involved in the progress of inflammation/stress-associated diseases. Our data suggested that javamide analogues may contain strong anti-inflammation activities, but there is little information about their effects on p38 MAPK. Therefore, in this paper, the effects of thirty javamide analogues on p38 MAPK were investigated using in silico screening and in vitro p38 MAPK assay methods. The javamide analogues were synthesized and their chemical structures were confirmed using nuclear magnetic resonance (NMR) spectroscopic methods...
December 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29235880/a-critical-review-of-physicochemical-properties-and-analytical-methods-applied-to-quantitative-determination-of-ebastine
#3
Rafael Giorgetti Frare, Anil Kumar Singh
Allergic diseases are the most common conditions in children and the second most frequent in adults. Currently, there are two well-defined generations of antihistamines, those belonging to first generation, with inherent side effects such as drowsiness and anticholinergic effects. These side effects are often attributed to their high lipophilicity and high affinity for brain H1 receptors. The ebastine is a modern antihistaminic drug belongs to the second generation and has lower lipophilicity, which diminish the undesirable side effects...
December 13, 2017: Critical Reviews in Analytical Chemistry
https://www.readbyqxmd.com/read/29235726/chemical-immobilization-effect-on-lithium-polysulfides-for-lithium-sulfur-batteries
#4
REVIEW
Caixia Li, Zhucong Xi, Dexiang Guo, Xiangju Chen, Longwei Yin
Despite great progress in lithium-sulfur batteries (LSBs), great obstacles still exist to achieve high loading content of sulfur and avoid the loss of active materials due to the dissolution of the intermediate polysulfide products in the electrolyte. Relationships between the intrinsic properties of nanostructured hosts and electrochemical performance of LSBs, especially, the chemical interaction effects on immobilizing polysulfides for LSB cathodes, are discussed in this Review. Moreover, the principle of rational microstructure design for LSB cathode materials with strong chemical interaction adsorbent effects on polysulfides, such as metallic compounds, metal particles, organic polymers, and heteroatom-doped carbon, is mainly described...
December 13, 2017: Small
https://www.readbyqxmd.com/read/29235545/one-plasmid-double-expression-his-tag-system-for-rapid-production-and-easy-purification-of-ms2-phage-like-particles
#5
Pavel Mikel, Petra Vasickova, Petr Kralik
MS2 phage-like particles (MS2 PLP) are artificially constructed pseudo-viral particles derived from bacteriophage MS2. They are able to carry a specific single stranded RNA (ssRNA) sequence of choice inside their capsid, thus protecting it against the effects of ubiquitous nucleases. Such particles are able to mimic ssRNA viruses and, thus, may serve as the process control for molecular detection and quantification of such agents in several kinds of matrices, vaccines and vaccine candidates, drug delivery systems, and systems for the display of immunologically active peptides or nanomachines...
December 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29235492/proteome-wide-analysis-of-lysine-2-hydroxyisobutyrylation-in-developing-rice-oryza-sativa-seeds
#6
Xiaoxi Meng, Shihai Xing, Loida M Perez, Xiaojun Peng, Qingyong Zhao, Edilberto D Redoña, Cailin Wang, Zhaohua Peng
Lysine 2-hydroxyisobutyrylation is a recently identified protein post-translational modification that is known to affect the association between histone and DNA. However, non-histone protein lysine 2-hydroxyisobutyrylation remains largely unexplored. Utilizing antibody-based affinity enrichment and nano-HPLC/MS/MS analyses of 2-hydroxyisobutyrylation peptides, we efficaciously identified 9,916 2-hydroxyisobutyryl lysine sites on 2,512 proteins in developing rice seeds, representing the first lysine 2-hydroxyisobutyrylome dataset in plants...
December 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29235277/-targets-identification-and-mechanism-analysis-for-macrophage-activation-of-low-molecular-weight-saccharides-from-cistanche-deserticola-based-on-molecular-affinity-chromatography
#7
Li-Chao Wang, Dan Liu, Yong Jiang, Peng-Fei Tu, Ke-Wu Zeng
This study aims to investigate the targets and targets-involved mechanism for the macrophage activation of low molecular weight saccharides from Cistanche deserticola (LMSC). The phagocytic activity and NO release of RAW264.7 cells were detected, and results showed that LMSC exerts immune activation effect by significantly increasing the phagocytic activity and NO release. LMSC-conjugated epoxy-activated sepharose beads were prepared as affinity reagent to capture the target proteins. Twenty-four proteins such as Eef2 were identified by LC-MS/MS analysis...
October 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29235109/-double-duty-conventional-dendritic-cells-in-the-amphibian-xenopus-as-the-prototype-for-antigen-presentation-to-b-cells
#8
Harold R Neely, Jacqueline Guo, Emily M Flowers, Michael F Criscitiello, Martin F Flajnik
Two populations of dendritic cells (DCs) are found in mammals, one derived from hematopoietic precursors (conventional/cDC), and another derived from mesenchymal precursors, the follicular DC (FDC); the latter is specialized for antigen presentation to B cells, and has only been definitively demonstrated in mammals. Both cDC and FDC are necessary for induction of germinal centers (GC) and GC-dependent class switch recombination (CSR) and somatic hypermutation (SHM). We demonstrate that in Xenopus, an amphibian in which immunoglobulin CSR and SHM occur without GC formation, a single type of DC has properties of both cDC and FDC, including high expression of MHC class II for the former and display of native antigen at the cell surface for the latter...
December 12, 2017: European Journal of Immunology
https://www.readbyqxmd.com/read/29234993/narrative-identity-in-third-party-reproduction-normative-aspects-and-ethical-challenges
#9
Natacha Salomé Lima
In the last few decades, assisted reproduction has introduced new challenges to the way people conceive and build their families. While the numbers of donor-conceived (DC) individuals have increased worldwide, there are still many controversies concerning access to donor information. Is there a fundamental moral right to know one's genetic background? What does identity in DC families mean? Is there any relationship between identity formation and disclosure of genetic origins? These questions are addressed by analysing core regulatory discourse (ethical recommendations and codes of practice)...
December 12, 2017: Journal of Bioethical Inquiry
https://www.readbyqxmd.com/read/29234980/low-dose-interleukin-2-as-a-modulator-of-treg-homeostasis-after-hsct-current-understanding-and-future-perspectives
#10
REVIEW
Ken-Ichi Matsuoka
CD4+CD25+Foxp3+ Treg is a functionally distinct subset of mature T cells with broad suppressive activity and has been shown to play an important role in the establishment of immune tolerance after HSCT. Altered cytokine environment in post-HSCT lymphopenia with a relative functional deficiency of IL-2 could hamper the reconstitution of Treg, leading to refractory GVHD. Based on the theory of low-dose IL-2 in which Treg can be selectively stimulated through the high-affinity IL-2 receptor, clinical studies have been conducted and demonstrated that low-dose IL-2 administration can restore Treg homeostasis and promote the expansion of this subset on the polymorphic processes of Treg reconstitution after HSCT...
December 12, 2017: International Journal of Hematology
https://www.readbyqxmd.com/read/29234830/temperature-dependent-adsorption-desorption-behaviour-of-pendimethalin-in-punjab-soils
#11
Pervinder Kaur, Amanpreet Makkar, Paawan Kaur, Shilpa
This study shows the effect of soil type and temperature on the adsorption and desorption behaviour of pendimethalin using a batch equilibration technique. Adsorption kinetics followed pseudo-second-order-model (R2 > 0.99). The shape of adsorption curve for studied soils was S-type at 30 and 40°C and L-type at 50°C. The isotherms were nonlinear and were well described by Freundlich equation. Adsorption capacity ranged from 1.4 to 2.2 μg1 - 1/n g-1 mL1/n and the order of adsorption was: clay loam > sandy loam > loamy sand indicating strong affinity of pendimethalin towards organic matter and clay content...
December 12, 2017: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/29234597/a-supervised-framework-with-intensity-subtraction-and-deformation-field-features-for-the-detection-of-new-t2-w-lesions-in-multiple-sclerosis
#12
Mostafa Salem, Mariano Cabezas, Sergi Valverde, Deborah Pareto, Arnau Oliver, Joaquim Salvi, Àlex Rovira, Xavier Lladó
Introduction: Longitudinal magnetic resonance imaging (MRI) analysis has an important role in multiple sclerosis diagnosis and follow-up. The presence of new T2-w lesions on brain MRI scans is considered a prognostic and predictive biomarker for the disease. In this study, we propose a supervised approach for detecting new T2-w lesions using features from image intensities, subtraction values, and deformation fields (DF). Methods: One year apart multi-channel brain MRI scans were obtained for 60 patients, 36 of them with new T2-w lesions...
2018: NeuroImage: Clinical
https://www.readbyqxmd.com/read/29234112/a-switch-in-nucleotide-affinity-governs-activation-of-the-src-and-tec-family-kinases
#13
Freia von Raußendorf, Anita de Ruiter, Thomas A Leonard
The Tec kinases, closely related to Src family kinases, are essential for lymphocyte function in the adaptive immune system. Whilst the Src and Abl kinases are regulated by tail phosphorylation and N-terminal myristoylation respectively, the Tec kinases are notable for the absence of either regulatory element. We have found that the inactive conformations of the Tec kinase Itk and Src preferentially bind ADP over ATP, stabilising both proteins. We demonstrate that Itk adopts the same conformation as Src and that the autoinhibited conformation of Src is independent of its C-terminal tail...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29233995/adeno-associated-virus-serotype-rh10-is-a-useful-gene-transfer-vector-for-sensory-nerves-that-innervate-bone-in-immunodeficient-mice
#14
Sun H Park, Matthew R Eber, Shunsuke Tsuzuki, Mary E Booker, Aaron G Sunil, D Brooke Widner, Renee A Parker, Christopher M Peters, Yusuke Shiozawa
Adeno-associated virus (AAV) is frequently used to manipulate gene expression in the sensory nervous system for the study of pain mechanisms. Although some serotypes of AAV are known to have nerve tropism, whether AAV can distribute to sensory nerves that innervate the bone or skeletal tissue has not been shown. This information is crucial, since bone pain, including cancer-induced bone pain, is an area of high importance in pain biology. In this study, we found that AAVrh10 transduces neurons in the spinal cord and dorsal root ganglia of immunodeficient mice with higher efficacy than AAV2, 5, 6, 8, and 9 when injected intrathecally...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29233890/phosphate-pi-regulated-heterodimerization-of-the-high-affinity-sodium-dependent-pi-transporters-pit1-slc20a1-and-pit2-slc20a2-underlies-extracellular-pi-sensing-independently-of-pi-uptake
#15
Nina Bon, Greig Couasnay, Annabelle Bourgine, Sophie Sourice, Sarah Beck-Cormier, Jérôme Guicheux, Laurent Beck
Extracellular phosphate (Pi) can act as a signaling molecule that directly alters gene expression and cellular physiology. The ability of cells or organisms to detect changes in extracellular Pi levels implies the existence of a Pi-sensing mechanism that signals to the body or individual cell. However, unlike in prokaryotes, yeasts, and plants, the molecular players involved in Pi sensing in mammals remain unknown. In this study, we investigated the involvement of the high-affinity, Na-dependent Pi transporters PiT1 and PiT2 in mediating Pi signaling in skeletal cells...
December 12, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29233847/pharmacological-evidence-for-a-putative-conserved-allosteric-site-on-opioid-receptors
#16
Kathryn E Livingston, M Alexander Stanczyk, Neil Burford, Andrew Alt, Merixtell Canals, John R Traynor
Allosteric modulators of G protein-coupled receptors (GPCRs), including opioid receptors, have been proposed as possible therapeutic agents with enhanced selectivity. BMS-986122 is a positive allosteric modulator (PAM) of the mu-opioid receptor (μ-OR). BMS-986187 is a structurally distinct PAM for the delta-opioid receptor (δ-OR) that has been reported to show 100-fold selectivity in promoting δ-OR over μ-OR agonism. Here we use ligand binding and second messenger assays to show that BMS-986187 is actually an effective PAM at μ-OR and at the kappa opioid receptor (κ-OR), but is ineffective at the nociceptin receptor (NOPR)...
December 12, 2017: Molecular Pharmacology
https://www.readbyqxmd.com/read/29233819/characterization-of-interactions-among-cyp1a2-cyp2b4-and-nadph-cytochrome-p450-reductase-identification-of-specific-protein-complexes
#17
John Patrick Connick, James R Reed, Wayne L Backes
Cytochromes P450 catalyze oxygenation reactions via interactions with their redox partners. However, other proteins, particularly other P450s, also have been shown to form complexes that modulate P450 function. Previous studies showed that CYP1A2 and CYP2B4 form a complex when reconstituted into phospholipid vesicles; however, details of the interactions among the P450s and NADPH-cytochrome P450 reductase have not been fully characterized. The goal of this study was to examine P450 complex formation in living cells, using bioluminescence resonance energy transfer (BRET)...
December 12, 2017: Drug Metabolism and Disposition: the Biological Fate of Chemicals
https://www.readbyqxmd.com/read/29233707/phylogenetic-characterization-of-transporter-proteins-in-the-cnidarian-dinoflagellate-symbiosis
#18
Ashley E Sproles, Nathan L Kirk, Sheila A Kitchen, Clinton A Oakley, Arthur R Grossman, Virginia M Weis, Simon K Davy
Metabolic exchange between cnidarians and their symbiotic dinoflagellates is central to maintaining their mutualistic relationship. Sugars are translocated to the host, while ammonium and nitrate are utilized by the dinoflagellates (Symbiodinium spp.). We investigated membrane protein sequences of each partner to identify potential transporter proteins that move sugars into cnidarian cells and nitrogen products into Symbiodinium cells. We examined the facilitated glucose transporters (GLUT), sodium/glucose cotransporters (SGLT), and aquaporin (AQP) channels in the cnidarian host as mechanisms for sugar uptake, and the ammonium and high-affinity nitrate transporters (AMT and NRT2, respectively) in the algal symbiont as mechanisms for nitrogen uptake...
December 9, 2017: Molecular Phylogenetics and Evolution
https://www.readbyqxmd.com/read/29233262/medicinal-chemistry-strategies-for-pet-tracer-discovery
#19
REVIEW
José Ignacio Andrés, Mark Schmidt
The detection of gamma rays, resulting from decay of positron emitting isotopes, allows exquisitely sensitive detection of probes radiolabeled with such isotopes. These probes can be designed for high affinity binding to specific molecular targets and be used as tools in the early development of drugs, particularly for neuropsychiatric disorders. Availability of novel tracers requires dedicated resources and selection assays. Many of the selection assays are similar to those used for discovery of clinical compounds, although the distribution and clearance of target specific radioligands requires different in vitro and in vivo methods and new derivatives...
November 2017: Drug Discovery Today. Technologies
https://www.readbyqxmd.com/read/29233046/how-is-a-new-category-born-on-mechanisms-of-formation-cycles-of-recognition-and-the-looping-effect-of-national-trauma
#20
Galia Plotkin Amrami
This article explores the mechanisms underlying the formation of a new category in the Israeli therapeutic field-"national trauma." By comparing the two different paths of emergence of this category, the research reexamines the meaning of Hacking's concept "looping effect" and, in particular, the issue of awareness of the categorized individuals and the categorizing knowledge-producers to the effects of a categorization. This study demonstrates that the formation of "national trauma" is both an intentional product of the efforts and ideology of practitioners and an unintentional outcome of their scientific and interventional activities...
March 1, 2017: Health (London)
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