Rebecca M Lennen, Hyun Gyu Lim, Kristian Jensen, Elsayed T Mohammed, Patrick V Phaneuf, Myung Hyun Noh, Sailesh Malla, Rosa A Börner, Ksenia Chekina, Emre Özdemir, Ida Bonde, Anna Koza, Jérôme Maury, Lasse E Pedersen, Lars Y Schöning, Nikolaus Sonnenschein, Bernhard O Palsson, Alex T Nielsen, Morten O A Sommer, Markus J Herrgård, Adam M Feist
Although strain tolerance to high product concentrations is a barrier to the economically viable biomanufacturing of industrial chemicals, chemical tolerance mechanisms are often unknown. To reveal tolerance mechanisms, an automated platform was utilized to evolve Escherichia coli to grow optimally in the presence of 11 industrial chemicals (1,2-propanediol, 2,3-butanediol, glutarate, adipate, putrescine, hexamethylenediamine, butanol, isobutyrate, coumarate, octanoate, hexanoate), reaching tolerance at concentrations 60%-400% higher than initial toxic levels...
February 2, 2023: Metabolic Engineering